##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2112_lane1_ACAGTG_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.8897515 31.0 28.0 31.0 16.0 34.0 2 30.3091656875 31.0 30.0 33.0 26.0 34.0 3 27.1728505625 31.0 25.0 31.0 16.0 34.0 4 31.7020975625 35.0 30.0 37.0 19.0 37.0 5 32.8931621875 35.0 32.0 37.0 26.0 37.0 6 34.2725394375 35.0 35.0 37.0 30.0 37.0 7 34.776348125 35.0 35.0 37.0 32.0 37.0 8 34.9787711875 35.0 35.0 37.0 32.0 37.0 9 36.6644383125 39.0 35.0 39.0 32.0 39.0 10-11 36.727950343749995 39.0 36.0 39.0 32.5 39.0 12-13 36.7616434375 39.0 36.0 39.0 32.5 39.0 14-15 37.88036515625 40.0 37.0 41.0 33.0 41.0 16-17 37.8783575625 40.0 37.0 41.0 32.5 41.0 18-19 37.898422625 39.5 37.0 41.0 33.0 41.0 20-21 37.996108125000006 40.0 37.0 41.0 33.0 41.0 22-23 37.897969625 40.0 37.0 41.0 33.0 41.0 24-25 37.94212884375 40.0 37.0 41.0 33.0 41.0 26-27 37.65928009375 40.0 37.0 41.0 32.0 41.0 28-29 37.49392815625 40.0 36.5 41.0 31.5 41.0 30-31 37.459203562499994 39.5 37.0 41.0 31.5 41.0 32-33 37.311259500000006 39.5 36.5 41.0 31.0 41.0 34-35 36.63401681249999 39.0 35.0 40.0 30.0 41.0 36-37 36.295520499999995 39.0 35.0 40.0 29.0 41.0 38-39 35.48759028125 38.0 33.5 40.0 26.0 41.0 40-41 36.4076436875 38.5 35.0 40.0 30.0 41.0 42-43 36.677011875 39.0 35.0 40.0 30.0 41.0 44-45 36.61233728125001 39.0 35.0 41.0 30.0 41.0 46-47 36.29829128125 39.0 35.0 40.0 30.0 41.0 48-49 36.341645312500006 39.0 35.0 40.0 30.0 41.0 50-51 36.14405509375 39.0 35.0 40.0 28.5 41.0 52-53 35.68416428125 38.0 34.0 40.0 27.5 41.0 54-55 35.56738584375 38.0 34.0 40.0 28.0 41.0 56-57 35.35234446875 38.0 34.0 40.0 28.0 41.0 58-59 34.95125390625 37.0 33.5 40.0 27.5 41.0 60-61 34.572122843749995 36.5 33.0 40.0 26.0 41.0 62-63 34.02424609375 36.0 33.0 39.5 26.0 40.5 64-65 32.899810625 35.0 32.0 38.0 24.5 39.5 66-67 32.72137484375 35.0 32.0 38.0 24.0 39.0 68-69 30.687275281250002 34.5 30.5 36.5 10.0 39.0 70-71 25.57852015625 33.0 11.0 35.0 2.0 37.5 72-73 24.241077625 33.0 2.0 35.0 2.0 37.0 74-75 23.92015109375 33.0 2.0 35.0 2.0 36.5 76-77 22.100807312500002 29.5 2.0 33.0 2.0 34.5 78-79 23.53344478125 32.5 2.0 35.0 2.0 35.5 80-81 23.656438968750003 33.0 2.0 35.0 2.0 35.0 82-83 23.55467471875 33.0 2.0 35.0 2.0 35.0 84-85 23.43278078125 33.0 2.0 35.0 2.0 35.0 86-87 23.30509453125 33.0 2.0 35.0 2.0 35.0 88-89 23.139341625 33.0 2.0 35.0 2.0 35.0 90-91 23.0047645625 32.5 2.0 35.0 2.0 35.0 92-93 22.83687475 32.0 2.0 35.0 2.0 35.0 94-95 22.654207218750003 32.0 2.0 35.0 2.0 35.0 96-97 22.44267621875 32.0 2.0 35.0 2.0 35.0 98-99 22.156428187499998 32.0 2.0 35.0 2.0 35.0 100-101 20.919268062500002 29.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 2.342546667788664 2306 2 1.35431887437327 2306 3 2.8132041690280367 2306 4 2.448774153075533 2306 5 2.605063593281102 2306 6 1.1734403457915334 2306 7 0.5438415448715972 2306 8 0.3744723658118261 2306 9 0.3975357746039805 2306 10-11 0.3719744698738481 2306 12-13 0.32564884669868377 2306 14-15 0.1743832218778536 2306 16-17 0.2777227676526053 2306 18-19 0.12043091493223557 2306 20-21 0.31438901510064454 2306 22-23 0.03213061850657084 2306 24-25 0.19257453161952753 2306 26-27 0.09462535443438469 2306 28-29 0.27307769107697055 2306 30-31 0.25398763248417566 2306 32-33 0.30152568304975347 2306 34-35 0.5029232026905248 2306 36-37 0.5183686159819274 2306 38-39 0.44932350229963447 2306 40-41 0.25831857232385147 2306 42-43 0.09430663000176054 2306 44-45 0.0678999872850099 2306 46-47 0.050595733860994585 2306 48-49 0.07643407177388184 2306 50-51 -0.08903621196776612 2306 52-53 -0.1220970751956969 2306 54-55 -0.30630291389552156 2306 56-57 -0.36651717130240513 2306 58-59 -0.49248872728159654 2306 60-61 -0.6518595212188316 2306 62-63 -0.6519374191522758 2306 64-65 -1.667995521980913 2306 66-67 -1.2768897863954933 2306 68-69 -2.045790633671711 2306 70-71 -0.38601708443738403 2306 72-73 0.37021857447121675 2306 74-75 0.43423423652423665 2306 76-77 -0.4877216788563423 2306 78-79 0.1765140460615413 2306 80-81 0.3148454460589889 2306 82-83 0.29356493358814717 2306 84-85 0.25520858950163117 2306 86-87 0.2647575195892955 2306 88-89 0.1923111078109656 2306 90-91 0.1473693446372195 2306 92-93 0.06912665612392033 2306 94-95 0.027099748137278112 2306 96-97 0.03989921406845198 2306 98-99 -0.07347936118993204 2306 100-101 -0.6785273441763913 2307 1 2.5840744696259748 2307 2 1.577404134055481 2307 3 3.766740254649374 2307 4 3.012296300029405 2307 5 2.8287167046066557 2307 6 1.4464121451124683 2307 7 0.6723661498966962 2307 8 0.3947282403501404 2307 9 0.4286989053589707 2307 10-11 0.41357904494943654 2307 12-13 0.4193168660043227 2307 14-15 0.22355288405641005 2307 16-17 0.31900481335170383 2307 18-19 0.27947147258770144 2307 20-21 0.5053839439241514 2307 22-23 0.3005336873647124 2307 24-25 0.3193168400599262 2307 26-27 0.36806973864335646 2307 28-29 0.41090729235678225 2307 30-31 0.4652932995799901 2307 32-33 0.49338159160998174 2307 34-35 0.7513562872496138 2307 36-37 0.9651936013102826 2307 38-39 0.845813905824798 2307 40-41 0.5379159922807801 2307 42-43 0.38112828811655675 2307 44-45 0.4457595094938682 2307 46-47 0.34093136015450654 2307 48-49 0.4105327531968186 2307 50-51 0.3169045197241047 2307 52-53 0.48268048035463806 2307 54-55 0.435718076721173 2307 56-57 0.23255553062293188 2307 58-59 0.2349388949769491 2307 60-61 0.15075802373144143 2307 62-63 0.18058812367478794 2307 64-65 -0.7292682358467033 2307 66-67 -0.3026470248894242 2307 68-69 -0.6198074341706992 2307 70-71 -0.3992457623989445 2307 72-73 0.1134589385680691 2307 74-75 0.1403958037427877 2307 76-77 -0.1747488540394464 2307 78-79 0.03961192480245401 2307 80-81 0.08451956307677477 2307 82-83 0.04982951126615731 2307 84-85 0.06916983627118967 2307 86-87 0.09571925605366971 2307 88-89 0.0029633556325308064 2307 90-91 -0.01156921325913629 2307 92-93 -0.10049681890820139 2307 94-95 -0.08140668687487107 2307 96-97 -0.05823461121503115 2307 98-99 -0.1308518563925567 2307 100-101 -0.48573767245826005 2201 1 1.4073446600024226 2201 2 0.6120021432335676 2201 3 1.704537763654976 2201 4 -0.5450471808515083 2201 5 0.5655138686443593 2201 6 0.2635784062199278 2201 7 0.15209212234591973 2201 8 0.09283048338467381 2201 9 0.12723152216652522 2201 10-11 0.2011598445441436 2201 12-13 0.10188910230258585 2201 14-15 0.08988891003863131 2201 16-17 0.11999611091201245 2201 18-19 0.08008606148857211 2201 20-21 0.08557113287757545 2201 22-23 0.15418184674112467 2201 24-25 0.12789213090337626 2201 26-27 0.14626696242595472 2201 28-29 0.22597973148625528 2201 30-31 0.1076922230571995 2201 32-33 0.12823966859665603 2201 34-35 0.28467091675801726 2201 36-37 0.35866859340863755 2201 38-39 0.2133560704601578 2201 40-41 0.07263415701854115 2201 42-43 -0.0454322426013718 2201 44-45 -0.052134622521691654 2201 46-47 -0.06428008596702028 2201 48-49 -0.00683770681612117 2201 50-51 -0.20992379464631483 2201 52-53 -0.1702609851375314 2201 54-55 -0.24983143277823672 2201 56-57 -0.323003649744912 2201 58-59 -0.4253523892264255 2201 60-61 -0.5609960038358466 2201 62-63 -0.5539641839663858 2201 64-65 -1.3537112654617118 2201 66-67 -0.9537365639805238 2201 68-69 -1.5829525174285273 2201 70-71 -0.5961919965406892 2201 72-73 0.2689709361634094 2201 74-75 0.31775043672770664 2201 76-77 -0.2603822314072808 2201 78-79 0.15016857726370958 2201 80-81 0.17465102970231783 2201 82-83 0.1688567823099767 2201 84-85 0.08096181856490503 2201 86-87 0.15660755561379602 2201 88-89 0.08508869747796055 2201 90-91 0.12193534987546073 2201 92-93 -0.006303917856183006 2201 94-95 -0.12221383433718813 2201 96-97 -0.08010768160891857 2201 98-99 -0.20240639920274006 2201 100-101 -0.5249293389752587 1101 1 0.8591341018708363 1101 2 0.25314282902791163 1101 3 0.9395415089278956 1101 4 -0.44483282589263595 1101 5 1.036540164402652 1101 6 0.4074677988589812 1101 7 0.1544245529724506 1101 8 0.12037173445745708 1101 9 0.08600056569155612 1101 10-11 0.08482032064363665 1101 12-13 0.06779726275189546 1101 14-15 -0.0026369202140799075 1101 16-17 -0.013141846544520774 1101 18-19 -0.047609119795779975 1101 20-21 0.049817018889868336 1101 22-23 -0.12244858529455627 1101 24-25 0.018318586581273166 1101 26-27 -0.03987532559715845 1101 28-29 0.042087867270346635 1101 30-31 0.005639626486271254 1101 32-33 0.0024347381798861534 1101 34-35 0.17693129257921925 1101 36-37 0.315374577859437 1101 38-39 0.3445450960523573 1101 40-41 0.054301652598518046 1101 42-43 -0.1296281259348575 1101 44-45 -0.18841577879510396 1101 46-47 -0.10837673220400745 1101 48-49 0.010992445815119822 1101 50-51 -0.1386056607525603 1101 52-53 -0.1835430067155528 1101 54-55 -0.26362348875616703 1101 56-57 -0.2710071564525336 1101 58-59 -0.3188669513125433 1101 60-61 -0.48539959060436644 1101 62-63 -0.5611931419516054 1101 64-65 -1.2016420653529423 1101 66-67 -0.943210881314819 1101 68-69 -1.614017863090428 1101 70-71 -0.20771336077024927 1101 72-73 0.6475287432631518 1101 74-75 0.6007346156031517 1101 76-77 0.0667713510650465 1101 78-79 0.5504746433927927 1101 80-81 0.6648181214397724 1101 82-83 0.6653148458674103 1101 84-85 0.704860829630892 1101 86-87 0.6782565472205455 1101 88-89 0.6032661850012992 1101 90-91 0.5547722702805764 1101 92-93 0.5283514563830103 1101 94-95 0.4462456858837456 1101 96-97 0.4646349357556012 1101 98-99 0.35774730861744786 1101 100-101 -0.05063237370773521 2304 1 2.2397680065135077 2304 2 1.1034541380655654 2304 3 2.30818636115907 2304 4 3.2307464475082597 2304 5 2.6251414303815466 2304 6 0.9770914464039819 2304 7 0.3496078936393374 2304 8 0.1632934212554602 2304 9 0.0471085979294017 2304 10-11 -0.06718252926697232 2304 12-13 -0.12508949610483455 2304 14-15 -0.0892780104203652 2304 16-17 -0.11733025714413259 2304 18-19 -0.10047483535697666 2304 20-21 -0.04383651644136677 2304 22-23 -0.20457030167930412 2304 24-25 -0.19379429873889364 2304 26-27 -0.20424602633094935 2304 28-29 -0.20054596463722163 2304 30-31 -0.08890060432791103 2304 32-33 0.027593723055197472 2304 34-35 0.02248667240446167 2304 36-37 -0.11408276403076911 2304 38-39 -0.13011335148542003 2304 40-41 -0.23139060934358469 2304 42-43 -0.34421888732579475 2304 44-45 -0.193461104130904 2304 46-47 -0.31682278417031995 2304 48-49 -0.3557426278827549 2304 50-51 -0.5093613908518435 2304 52-53 -0.3925586743568772 2304 54-55 -0.4987545448277615 2304 56-57 -0.44224969444469053 2304 58-59 -0.5129465644159552 2304 60-61 -0.7436349340103163 2304 62-63 -0.5835499590250492 2304 64-65 -1.3674935321925261 2304 66-67 -1.0669435944473982 2304 68-69 -1.4393152217374272 2304 70-71 -0.15757249731458955 2304 72-73 0.729261728675084 2304 74-75 0.8486997878035254 2304 76-77 0.22929331440426992 2304 78-79 0.7151458168031795 2304 80-81 0.7954179803853947 2304 82-83 0.8721344782865863 2304 84-85 0.8427661373261195 2304 86-87 0.815360995791572 2304 88-89 0.8310620223543879 2304 90-91 0.7435624042711488 2304 92-93 0.7660123331564002 2304 94-95 0.7084005078288484 2304 96-97 0.6157157234294104 2304 98-99 0.5131861241178868 2304 100-101 0.16131072193976692 2202 1 1.9495406352610622 2202 2 1.0049986996904323 2202 3 1.505047837639136 2202 4 2.9414476983132474 2202 5 2.0313748016027944 2202 6 0.7906024757323777 2202 7 0.30718886761378883 2202 8 0.18999005962166393 2202 9 -0.020412898522863543 2202 10-11 -0.051262971652413114 2202 12-13 -0.12807796329456522 2202 14-15 -0.30170338832515853 2202 16-17 -0.2528519533922946 2202 18-19 -0.15825606603365827 2202 20-21 -0.07851578046994234 2202 22-23 -0.25092401191394487 2202 24-25 -0.18023589543042817 2202 26-27 -0.23385749060233252 2202 28-29 -0.36584255813725264 2202 30-31 -0.27836831666853357 2202 32-33 -0.20523060142060245 2202 34-35 -0.09434852198966581 2202 36-37 0.031335811350984955 2202 38-39 -0.027477896062123364 2202 40-41 -0.18085131382223807 2202 42-43 -0.3107416517463264 2202 44-45 -0.3038629281570735 2202 46-47 -0.46477865104505867 2202 48-49 -0.4989907128370277 2202 50-51 -0.5692368615219721 2202 52-53 -0.5335118807613952 2202 54-55 -0.5051091591942907 2202 56-57 -0.594993591383691 2202 58-59 -0.7071008999304809 2202 60-61 -0.7386486029861317 2202 62-63 -0.7501594474311943 2202 64-65 -0.9574033789110388 2202 66-67 -0.9144937979269017 2202 68-69 -1.0451093409630836 2202 70-71 0.08378477060175982 2202 72-73 0.854682642996984 2202 74-75 0.9815503840630484 2202 76-77 0.2997026214263734 2202 78-79 0.8798819414777945 2202 80-81 0.9462342477627885 2202 82-83 0.9609108399207962 2202 84-85 0.9023375944894099 2202 86-87 0.8469843578741845 2202 88-89 0.8537495233671102 2202 90-91 0.9307685747058798 2202 92-93 0.922426603542295 2202 94-95 0.8442631544060752 2202 96-97 0.7878729915159681 2202 98-99 0.8141233093367362 2202 100-101 0.5698454889854361 1102 1 1.4015849576127692 1102 2 0.6989053386459467 1102 3 -0.0634325727811742 1102 4 0.19145800945991098 1102 5 1.4649509757158583 1102 6 0.5633371663934312 1102 7 0.15662531922036038 1102 8 0.008939130654681549 1102 9 -0.10157430007951973 1102 10-11 -0.05825947413610777 1102 12-13 -0.19027669525090118 1102 14-15 -0.31439329994561405 1102 16-17 -0.2777762789055629 1102 18-19 -0.20560240611056457 1102 20-21 -0.17450245745333604 1102 22-23 -0.3527056721438129 1102 24-25 -0.3011996052111243 1102 26-27 -0.34593376210577986 1102 28-29 -0.40828972608964875 1102 30-31 -0.2614189259591626 1102 32-33 -0.3010332004515419 1102 34-35 -0.3227482232260641 1102 36-37 -0.10484855330091136 1102 38-39 0.015663687790443248 1102 40-41 -0.22914508823382818 1102 42-43 -0.38805899504220065 1102 44-45 -0.3767942875476109 1102 46-47 -0.30572013340272974 1102 48-49 -0.2872383923595976 1102 50-51 -0.41108775650280904 1102 52-53 -0.4296206030526264 1102 54-55 -0.5671982635204813 1102 56-57 -0.5969460933337771 1102 58-59 -0.4719855909743984 1102 60-61 -0.5352203000166256 1102 62-63 -0.4031019749568543 1102 64-65 -0.7916322167751346 1102 66-67 -0.6266446004345383 1102 68-69 -0.707182093268802 1102 70-71 -0.11546072308681588 1102 72-73 0.56299151871292 1102 74-75 0.69031976271825 1102 76-77 0.3529679968364583 1102 78-79 0.6436449487567977 1102 80-81 0.7816262721605263 1102 82-83 0.7603679020851395 1102 84-85 0.7556645331221397 1102 86-87 0.7367690319690468 1102 88-89 0.7606995134696426 1102 90-91 0.7639561598564555 1102 92-93 0.6777982151262556 1102 94-95 0.654768880869856 1102 96-97 0.665701893256653 1102 98-99 0.6779081872780814 1102 100-101 0.5499662862176535 2305 1 1.8989865204062575 2305 2 0.8983193608577196 2305 3 3.325338549853875 2305 4 0.598879440246634 2305 5 0.9819960339802591 2305 6 0.3692191704859411 2305 7 0.08566364901303558 2305 8 0.015768662326259175 2305 9 -0.2259078383538622 2305 10-11 -0.2815309077621535 2305 12-13 -0.172945590180845 2305 14-15 -0.29788250189635335 2305 16-17 -0.32359424469679254 2305 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48-49 0.4802660820104663 1213 50-51 0.5289088539115028 1213 52-53 0.5971163841765872 1213 54-55 0.633571980902488 1213 56-57 0.6360330896978539 1213 58-59 0.7116107733312731 1213 60-61 0.8357078843335657 1213 62-63 0.8004171123506012 1213 64-65 1.3125895973615442 1213 66-67 1.0892808017799709 1213 68-69 1.3780401903075763 1213 70-71 -0.21019828896517367 1213 72-73 -0.5800823303959142 1213 74-75 -0.572137668266727 1213 76-77 0.03337192441379955 1213 78-79 -0.4271387926060477 1213 80-81 -0.5449610875576276 1213 82-83 -0.5576353062889261 1213 84-85 -0.5424534664916045 1213 86-87 -0.44850549340489465 1213 88-89 -0.4336641837386743 1213 90-91 -0.40951127884920524 1213 92-93 -0.3739557163764822 1213 94-95 -0.30702252613203385 1213 96-97 -0.32678057475646227 1213 98-99 -0.28791403980763874 1213 100-101 -0.0075891163667343164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3874.0 3 323.0 4 455.0 5 862.0 6 1707.0 7 4109.0 8 7459.0 9 11692.0 10 16751.0 11 22586.0 12 29235.0 13 37962.0 14 43989.0 15 49283.0 16 56581.0 17 66097.0 18 83109.0 19 110705.0 20 147408.0 21 195053.0 22 254693.0 23 335722.0 24 463473.0 25 689825.0 26 1059757.0 27 1460253.0 28 436785.0 29 366940.0 30 435786.0 31 526736.0 32 642085.0 33 797662.0 34 1031914.0 35 1392548.0 36 1967062.0 37 2319655.0 38 893708.0 39 36143.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.219612078166435 18.609610579875028 9.505293181623985 32.66548416033456 2 17.510523891342814 42.83249090117011 16.565189373791746 23.09179583369533 3 12.556415325804892 13.590550195803145 43.23232666688626 30.6207078115057 4 15.39794268206185 13.187122569645274 10.97088921933671 60.44404552895617 5 44.16287341616297 16.31313551138725 12.412844822252126 27.11114625019766 6 43.47908423954212 20.305091402545703 14.233932116966058 21.98189224094612 7 10.081921270480318 53.747794686948666 17.096248024062007 19.074036018509005 8 10.92853661157015 53.179050252740886 16.41019243582946 19.482220699859493 9 35.206229400778675 27.58205344775668 17.203077150384644 20.00864000108 10-11 22.460157653759854 39.58703997419 18.351932396995775 19.600869975054376 12-13 9.572444946555619 41.293902036737755 17.315861539482693 31.817791477223935 14-15 10.517275490313448 38.20437563275412 17.67859982701869 33.59974904991373 16-17 22.886857074928525 25.39960263096439 17.789878411475108 33.923661882631976 18-19 11.771484207274991 24.819665196704843 30.272200021475047 33.136650574545115 20-21 24.809868654934327 24.43391221695611 30.134827567413787 20.62139156069578 22-23 9.40676674252742 50.19365710935342 18.143308635932073 22.256267512187087 24-25 9.7186256074141 25.726476607904786 30.351583146973947 34.203314637707166 26-27 10.595600517237678 24.633027217603107 19.054981550149908 45.71639071500931 28-29 22.675893027679084 38.02969533908602 16.97315716947151 22.321254463763392 30-31 9.575937705254715 24.71749107512111 31.104853725361544 34.60171749426264 32-33 9.762688802940877 38.20358553517168 17.53133479989744 34.50239086199001 34-35 10.984845359819579 36.982626464294796 18.37922938287179 33.65329879301384 36-37 23.62822998322434 38.08582542701494 17.67704583390703 20.608898755853684 38-39 22.499584060888235 24.20234690909427 31.188670856099566 22.109398173917924 40-41 9.874245629087794 36.43997367237001 31.76317558067943 21.92260511786277 42-43 10.723548576531869 23.969473063356396 31.566030424743918 33.74094793536782 44-45 22.86308989478271 24.126574887234156 19.162643206653982 33.84769201132915 46-47 11.013361367850942 36.08110921232863 30.772717799034098 22.132811620786335 48-49 23.687780603376847 24.174990674814612 30.306055491276148 21.831173230532396 50-51 10.164041055428829 23.54286859149673 18.701302314009393 47.591788039065044 52-53 22.437263185257063 23.37572981371264 32.59236499596727 21.59464200506303 54-55 9.860851894091411 24.200955138109638 31.8700920168422 34.06810095095675 56-57 10.621758282040068 23.361985061476137 30.808834363573663 35.20742229291013 58-59 22.737910992353555 23.46685225095692 32.52769272091559 21.267544035773934 60-61 10.743279233659745 25.450360212232013 31.06882102857557 32.737539525532675 62-63 22.56541329492305 26.090767419409715 30.328736174429288 21.015083111237946 64-65 9.685721047386298 51.61903272009516 17.829564802387324 20.865681430131218 66-67 9.73524766448308 52.58437205267359 17.417949870216074 20.26243041262726 68-69 9.76342141378646 51.4054951292644 17.588336184819823 21.24274727212931 70-71 10.042116294172367 49.17173517313225 18.573079165764188 22.2130693669312 72-73 10.811555913000403 46.54373361170031 19.538140730408323 23.10656974489096 74-75 11.41517467018285 43.208231088068786 20.097696076844354 25.278898164904014 76-77 11.724740105118926 40.74773598358323 21.281549623667868 26.24597428762997 78-79 12.246775584130502 39.87691870959178 21.49047868290038 26.385827023377345 80-81 12.781660357138827 37.91568671612225 21.677796167307548 27.624856759431378 82-83 12.928308780329997 36.696017893366815 22.026276538669045 28.349396787634145 84-85 13.562228615864502 36.16957632668459 22.198670718519754 28.069524338931153 86-87 13.830362234940994 35.19252686332322 22.23640349438046 28.740707407355327 88-89 14.258267426570253 34.282431555645026 22.252692839118914 29.206608178665803 90-91 14.568810697258094 34.58043510822112 22.29344709535195 28.55730709916884 92-93 14.825273500926547 33.97439929889963 21.982958553446732 29.217368646727092 94-95 15.0651407471389 33.85770723320106 21.853919101098317 29.22323291856172 96-97 15.52984141100415 33.46387908639473 21.836181923916396 29.17009757868472 98-99 15.834002553379948 32.94366695335472 21.424832135612263 29.79749835765307 100-101 16.6527368881878 32.180141426548694 21.074893895400752 30.09222778986275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.5 6 2.5 7 3.0 8 3.5 9 11.0 10 14.0 11 19.5 12 33.0 13 46.0 14 73.0 15 123.0 16 226.0 17 369.0 18 586.5 19 872.5 20 1219.5 21 1637.5 22 2164.5 23 2891.0 24 3928.0 25 5344.5 26 7434.5 27 10901.0 28 16649.0 29 26188.5 30 42292.5 31 68010.0 32 108862.0 33 169892.0 34 250831.5 35 350591.0 36 466955.0 37 592767.0 38 721184.5 39 845601.0 40 957962.0 41 1048786.5 42 1112524.5 43 1141367.5 44 1125171.5 45 1067062.0 46 974519.0 47 860835.0 48 739125.0 49 635740.5 50 565843.5 51 489845.5 52 370530.0 53 254837.0 54 182310.5 55 134329.0 56 102249.0 57 80350.5 58 64957.0 59 53919.0 60 45985.0 61 40101.0 62 35561.0 63 31664.5 64 28128.0 65 24851.5 66 22052.0 67 19605.5 68 17156.5 69 14667.5 70 12340.5 71 10272.5 72 8408.5 73 6770.5 74 5333.0 75 4097.0 76 3111.0 77 2325.5 78 1701.5 79 1315.5 80 1002.5 81 729.5 82 550.0 83 433.0 84 342.0 85 267.5 86 240.5 87 201.5 88 146.5 89 127.0 90 113.0 91 99.0 92 79.5 93 67.0 94 59.0 95 43.0 96 28.5 97 15.0 98 7.5 99 4.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01110625 2 0.0043124999999999995 3 0.00639375 4 0.013631250000000001 5 5.625000000000001E-4 6 4.9999999999999996E-5 7 2.4999999999999998E-5 8 1.0625E-4 9 1.2499999999999999E-5 10-11 6.2499999999999995E-6 12-13 1.2499999999999999E-5 14-15 3.4375E-5 16-17 7.1875E-5 18-19 2.3750000000000003E-4 20-21 4.9999999999999996E-5 22-23 4.4375E-4 24-25 6.2499999999999995E-6 26-27 3.4375E-5 28-29 3.0000000000000003E-4 30-31 3.9375E-4 32-33 1.0937499999999999E-4 34-35 9.249999999999999E-4 36-37 2.0625000000000003E-4 38-39 3.8750000000000004E-4 40-41 4.937499999999999E-4 42-43 0.005115625 44-45 0.0057374999999999995 46-47 0.007196875 48-49 0.01874375 50-51 0.0193125 52-53 0.001771875 54-55 0.00268125 56-57 5.78125E-4 58-59 5.15625E-4 60-61 3.59375E-4 62-63 7.375E-4 64-65 9.593749999999999E-4 66-67 0.0020625 68-69 9.874999999999999E-4 70-71 8.46875E-4 72-73 1.125E-4 74-75 4.6249999999999997E-4 76-77 0.003140625 78-79 5.40625E-4 80-81 0.0015968749999999998 82-83 0.00205 84-85 4.875E-4 86-87 1.5625E-4 88-89 0.0015687499999999998 90-91 3.0937499999999997E-4 92-93 5.3125E-5 94-95 1.875E-4 96-97 0.001534375 98-99 1.9375000000000002E-4 100-101 6.531250000000001E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.260314592480206 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34106413536882 29.527343146372164 2 14.934360668610177 12.025241175116177 3 5.323525239454266 6.429804026443119 4 2.366609435984323 3.811217616410439 5 1.1189355543269737 2.252434871295759 6 0.5963583482546221 1.4405744826349776 7 0.43594955891946624 1.2286026471985487 8 0.3471576136576855 1.1181339791226497 9 0.23470658875026346 0.8504424990012135 >10 1.173876856940579 8.506011701401032 >50 0.0807968749655276 2.2089006030557123 >100 0.04110891098837725 3.0184311446227237 >500 0.003337237671145255 0.9186210947660487 >1k 0.0016609371986033383 1.1282844175086602 >5k 2.2257647932067478E-4 0.5866538704306197 >10k+ 3.2946242993365214E-4 24.949302724620196 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 3211126 20.069537500000003 TruSeq Adapter, Index 5 (100% over 50bp) CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT 197219 1.23261875 TruSeq Adapter, Index 5 (100% over 50bp) GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 92602 0.5787625000000001 TruSeq Adapter, Index 5 (98% over 50bp) GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 63231 0.39519374999999995 TruSeq Adapter, Index 5 (98% over 50bp) ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC 58719 0.36699375 No Hit GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 38250 0.23906249999999998 TruSeq Adapter, Index 5 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC 36378 0.2273625 TruSeq Adapter, Index 5 (98% over 50bp) AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA 30948 0.19342499999999999 No Hit GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT 30067 0.18791875 TruSeq Adapter, Index 5 (100% over 50bp) GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT 21207 0.13254375 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACTCGTATGCC 20942 0.1308875 TruSeq Adapter, Index 5 (97% over 40bp) CGTAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT 20940 0.130875 TruSeq Adapter, Index 5 (98% over 50bp) GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG 20290 0.1268125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC 18694 0.1168375 TruSeq Adapter, Index 5 (98% over 50bp) TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA 16302 0.1018875 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC 16231 0.10144375 TruSeq Adapter, Index 5 (98% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.8125E-4 0.0 0.0 2 7.875E-4 0.0 0.0 3 9.0E-4 0.0 0.0 4 0.0010375 0.0 0.0 5 0.001225 0.0 0.0 6 0.00154375 1.875E-5 0.0 7 0.00205625 1.875E-5 0.0 8 0.0032125 1.875E-5 0.0 9 0.0035625 3.125E-5 6.25E-6 10-11 0.006368749999999999 3.4374999999999995E-5 6.25E-6 12-13 0.011125 3.75E-5 9.375000000000001E-6 14-15 0.019615625 3.75E-5 1.25E-5 16-17 0.02406875 3.75E-5 1.25E-5 18-19 0.025775 3.75E-5 1.875E-5 20-21 0.02804375 3.75E-5 1.875E-5 22-23 0.03919375 3.75E-5 1.875E-5 24-25 0.063078125 3.75E-5 1.875E-5 26-27 0.078384375 5.0E-5 2.4999999999999998E-5 28-29 0.0819125 5.625E-5 3.125E-5 30-31 0.08454375 5.625E-5 3.75E-5 32-33 0.08819375 5.625E-5 4.0625E-5 34-35 0.091940625 5.625E-5 4.375E-5 36-37 0.096240625 5.625E-5 4.375E-5 38-39 0.1012875 5.625E-5 4.375E-5 40-41 0.107578125 6.874999999999999E-5 4.375E-5 42-43 0.11490625 7.5E-5 5.0E-5 44-45 0.12504375 7.5E-5 5.0E-5 46-47 0.1362625 8.125E-5 5.625E-5 48-49 0.14968437499999998 8.125E-5 6.25E-5 50-51 0.165465625 8.75E-5 6.25E-5 52-53 0.184621875 9.375E-5 6.25E-5 54-55 0.207421875 9.375E-5 6.25E-5 56-57 0.23525000000000001 9.375E-5 6.25E-5 58-59 0.27069375 9.375E-5 6.25E-5 60-61 0.314028125 9.375E-5 6.25E-5 62-63 0.368809375 9.375E-5 6.25E-5 64-65 0.436821875 9.375E-5 6.25E-5 66-67 0.52119375 9.375E-5 6.25E-5 68-69 0.6242937500000001 9.999999999999999E-5 6.875E-5 70-71 0.756525 1.0625E-4 6.875E-5 72-73 0.91810625 1.0625E-4 7.500000000000001E-5 74-75 1.113003125 1.0625E-4 8.125E-5 76-77 1.35360625 1.0625E-4 8.125E-5 78-79 1.6412125 1.125E-4 8.125E-5 80-81 1.9832687500000001 1.125E-4 8.125E-5 82-83 2.400196875 1.125E-4 8.75E-5 84-85 2.89605625 1.125E-4 8.75E-5 86-87 3.48640625 1.125E-4 9.375E-5 88-89 4.1853875 1.15625E-4 9.687500000000001E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGGA 20625 0.0 85.34979 1 CCGGAAG 21285 0.0 84.90309 3 ACCGGAA 22325 0.0 81.07939 2 GAGCACA 515710 0.0 77.03917 9 AGAGCAC 518565 0.0 76.65441 8 GAAGAGC 523600 0.0 76.04157 6 AAGAGCA 524775 0.0 75.83149 7 TCGGAAG 470310 0.0 75.11445 3 GGAAGAG 526045 0.0 74.76898 5 CGGAAGA 515290 0.0 74.27045 4 ATCGGAA 479205 0.0 73.72759 2 GATCGGA 481360 0.0 72.367386 1 ATTGGAA 14500 0.0 70.44456 2 TTGGAAG 14545 0.0 70.38663 3 GATTGGA 14805 0.0 68.74155 1 TGGAAGA 16265 0.0 66.765015 4 CATCGGA 6790 0.0 66.685486 1 ACGGAAG 11240 0.0 65.93483 3 TCCGAAG 6415 0.0 60.948044 3 CGAAGAG 7885 0.0 59.031796 5 >>END_MODULE