##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2112_lane1_ACAGTG_L001_R1_002.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1327210 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.236426790033228 30.0 25.0 31.0 16.0 33.0 2 28.653832475644396 31.0 28.0 31.0 16.0 34.0 3 24.65810534881443 28.0 16.0 31.0 10.0 33.0 4 29.808204428839446 33.0 28.0 35.0 19.0 37.0 5 32.26074170628612 35.0 32.0 35.0 25.0 37.0 6 33.903743190602846 35.0 33.0 37.0 30.0 37.0 7 34.55925889648209 35.0 35.0 37.0 32.0 37.0 8 34.80552587759284 35.0 35.0 37.0 32.0 37.0 9 36.50994266167373 38.0 35.0 39.0 32.0 39.0 10-11 36.64335636410214 38.5 35.0 39.0 32.0 39.0 12-13 36.622182623699345 39.0 35.0 39.0 32.0 39.0 14-15 37.81283444217569 40.0 37.0 41.0 33.0 41.0 16-17 37.808808703973 40.0 36.5 41.0 32.5 41.0 18-19 37.84213462828038 39.5 37.0 41.0 32.5 41.0 20-21 37.82591112182699 40.0 37.0 41.0 32.0 41.0 22-23 37.74397043421915 40.0 37.0 41.0 32.0 41.0 24-25 37.83964218171955 40.0 37.0 41.0 33.0 41.0 26-27 37.61831511215256 40.0 37.0 41.0 32.0 41.0 28-29 37.44070531415525 40.0 36.5 41.0 31.5 41.0 30-31 37.41327370951093 39.5 37.0 41.0 31.0 41.0 32-33 37.32637751373181 40.0 36.0 41.0 31.0 41.0 34-35 36.26347563686229 39.0 35.0 40.0 29.0 41.0 36-37 35.98083046390549 38.5 35.0 40.0 28.0 41.0 38-39 35.212861566745275 38.0 33.5 40.0 25.0 41.0 40-41 36.29139096299757 38.5 35.0 40.0 30.0 41.0 42-43 36.671942646604535 39.0 35.0 40.0 30.0 41.0 44-45 36.62010194317403 39.0 35.0 40.0 30.0 41.0 46-47 36.33623352747493 39.0 35.0 40.0 30.0 41.0 48-49 36.35864331944455 39.0 35.0 40.0 30.0 41.0 50-51 36.25588527813986 39.0 35.0 40.0 29.5 41.0 52-53 35.76669253546914 38.0 34.0 40.0 28.0 41.0 54-55 35.731386517581996 38.0 34.0 40.0 28.0 41.0 56-57 35.57887561124464 38.0 34.0 40.0 28.0 41.0 58-59 35.27875656452257 37.5 34.0 40.0 28.0 41.0 60-61 35.03426172195809 37.0 34.0 40.0 28.0 41.0 62-63 34.4107869139021 36.0 33.0 39.5 26.5 40.5 64-65 33.860936475764944 36.0 33.0 39.0 26.0 39.5 66-67 33.451303862990784 35.5 33.0 39.0 26.0 39.0 68-69 31.89841961709149 35.0 32.0 36.5 22.5 38.5 70-71 26.97569337181004 33.0 22.5 35.0 2.0 37.0 72-73 23.888408013803392 33.0 2.0 35.0 2.0 37.0 74-75 23.33185554659775 33.0 2.0 35.0 2.0 36.5 76-77 22.17830750220387 30.0 2.0 33.5 2.0 35.0 78-79 23.150087401390884 32.0 2.0 35.0 2.0 36.0 80-81 23.19999623269867 33.0 2.0 35.0 2.0 35.0 82-83 23.10732815454977 33.0 2.0 35.0 2.0 35.0 84-85 22.988109266807815 33.0 2.0 35.0 2.0 35.0 86-87 22.865338944100785 32.5 2.0 35.0 2.0 35.0 88-89 22.741499838006042 32.0 2.0 35.0 2.0 35.0 90-91 22.606066485333898 32.0 2.0 35.0 2.0 35.0 92-93 22.483696250028252 32.0 2.0 35.0 2.0 35.0 94-95 22.351372051144885 32.0 2.0 35.0 2.0 35.0 96-97 22.17809577986905 32.0 2.0 35.0 2.0 35.0 98-99 21.928005364637094 32.0 2.0 35.0 2.0 35.0 100-101 21.07076084417688 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2310 1 1.8262401464 2310 2 0.7289096816642662 2310 3 -0.8780301974195766 2310 4 -1.512067981293086 2310 5 -1.02624046635421 2310 6 -0.33880902324295903 2310 7 -0.017018121078002935 2310 8 0.05475686569248239 2310 9 0.22687107305566911 2310 10-11 0.2542319795676349 2310 12-13 0.2826452203132135 2310 14-15 0.37138761879453597 2310 16-17 0.3409649873137326 2310 18-19 0.3133235181096339 2310 20-21 0.46269835738897314 2310 22-23 0.3146399898973513 2310 24-25 0.2933182100655003 2310 26-27 0.37672579604456047 2310 28-29 0.46047840273938334 2310 30-31 0.40993256048049176 2310 32-33 0.3810894281379973 2310 34-35 0.37737046266462926 2310 36-37 0.533544356295387 2310 38-39 0.35152204140629806 2310 40-41 0.4501155354083366 2310 42-43 0.5001258078353388 2310 44-45 0.4882624131543096 2310 46-47 0.5074439715099643 2310 48-49 0.32600592047390364 2310 50-51 0.2503512760541753 2310 52-53 0.3663512146525818 2310 54-55 0.2389176173810057 2310 56-57 0.2264511290611182 2310 58-59 0.2531437924251847 2310 60-61 0.2891587248310259 2310 62-63 0.41479369130085786 2310 64-65 0.3157998020961159 2310 66-67 0.21721789219625265 2310 68-69 -0.027655092758813282 2310 70-71 -1.4959900538818793 2310 72-73 -0.3240116054208073 2310 74-75 -0.06726501346762248 2310 76-77 -0.09516696910373668 2310 78-79 -0.12725822804342357 2310 80-81 -0.13356613876988632 2310 82-83 -0.1599705866452794 2310 84-85 -0.18363857151461715 2310 86-87 -0.13446998445079217 2310 88-89 -0.14344431328421337 2310 90-91 -0.17748533398795985 2310 92-93 -0.22299917783484702 2310 94-95 -0.14418226468443862 2310 96-97 -0.2593358421219705 2310 98-99 -0.23476846332050627 2310 100-101 -0.3668425807289708 2311 1 2.4203493837476415 2311 2 1.9175502745374509 2311 3 -0.46242328490467344 2311 4 -0.6583738282775968 2311 5 -0.6254268932497062 2311 6 -0.1484065462647024 2311 7 0.07364570685840022 2311 8 0.20808877479857557 2311 9 0.3279445726067536 2311 10-11 0.28849324748614436 2311 12-13 0.2674911947863947 2311 14-15 0.32273792568081916 2311 16-17 0.34090243568851974 2311 18-19 0.2851056285729996 2311 20-21 0.4222570283503799 2311 22-23 0.32806280736542703 2311 24-25 0.3205814795933648 2311 26-27 0.2705878326533977 2311 28-29 0.26878209393461816 2311 30-31 0.30957473938097024 2311 32-33 0.346154652001907 2311 34-35 0.5839977799003222 2311 36-37 0.5759886115110504 2311 38-39 0.5800248129982748 2311 40-41 0.5224128936260328 2311 42-43 0.38943401362335806 2311 44-45 0.2535191026755754 2311 46-47 0.3922548311331795 2311 48-49 0.38617843543585195 2311 50-51 0.30948822654936947 2311 52-53 0.31706286374096493 2311 54-55 0.3129781551989481 2311 56-57 0.3765552239646297 2311 58-59 0.43491041857572554 2311 60-61 0.34778559142632304 2311 62-63 0.469231859459164 2311 64-65 0.2308661252649955 2311 66-67 0.18567521824387256 2311 68-69 0.04984028792122075 2311 70-71 -1.4347553341675408 2311 72-73 -0.3852889485258899 2311 74-75 -0.14466246963968743 2311 76-77 -0.11822343524964296 2311 78-79 -0.18389448366272632 2311 80-81 -0.15983395086895769 2311 82-83 -0.1634155962123245 2311 84-85 -0.13954511894938193 2311 86-87 -0.15783020956285654 2311 88-89 -0.19766791438719622 2311 90-91 -0.19845736971692673 2311 92-93 -0.16440569556385398 2311 94-95 -0.1745987908014257 2311 96-97 -0.18835126834542848 2311 98-99 -0.23612238906327576 2311 100-101 -0.24275458267824845 2309 1 2.3037143687403727 2309 2 1.276605325265919 2309 3 2.341078398284644 2309 4 1.4823561795753086 2309 5 1.8746752580842738 2309 6 0.8829502300113816 2309 7 0.4881144708809373 2309 8 0.4100019600340872 2309 9 0.397890173421942 2309 10-11 0.3471994430969687 2309 12-13 0.27079156962002315 2309 14-15 0.316859233660324 2309 16-17 0.35087193101292513 2309 18-19 0.23834279880546205 2309 20-21 0.3444215883890607 2309 22-23 0.3534705378821599 2309 24-25 0.34345423693292076 2309 26-27 0.3682516872916537 2309 28-29 0.44683222870398964 2309 30-31 0.3833286422837787 2309 32-33 0.30394592435252576 2309 34-35 0.42593074677768783 2309 36-37 0.4230043849457843 2309 38-39 0.4023403591356214 2309 40-41 0.32383816963348266 2309 42-43 0.3601842812441447 2309 44-45 0.4141301892842435 2309 46-47 0.40994980504068934 2309 48-49 0.39812128995365015 2309 50-51 0.43075246081426144 2309 52-53 0.43811361716769426 2309 54-55 0.40475014445740953 2309 56-57 0.46280523311772725 2309 58-59 0.4028076673419605 2309 60-61 0.3924426776295107 2309 62-63 0.32649543974608974 2309 64-65 0.28474562477852317 2309 66-67 0.22275465153072815 2309 68-69 0.11866370788515113 2309 70-71 -0.8286360067449294 2309 72-73 -0.3563833039492579 2309 74-75 -0.12717617891826194 2309 76-77 -0.07567851231455336 2309 78-79 -0.09417254141159503 2309 80-81 -0.08343044246465325 2309 82-83 -0.09774890164783656 2309 84-85 -0.08393782369688552 2309 86-87 -0.03412798270634809 2309 88-89 0.005728052379613047 2309 90-91 -0.012690966339583554 2309 92-93 -0.06964058304361131 2309 94-95 -0.0016069654500938668 2309 96-97 0.026821029640593963 2309 98-99 0.06453826695513953 2309 100-101 0.024968999156410376 2314 1 -0.046118763458988354 2314 2 0.892479809363401 2314 3 -0.7444007999120288 2314 4 -1.4703086290197902 2314 5 0.5767831580709029 2314 6 0.25081593268064495 2314 7 0.16098260891040184 2314 8 0.1352943555151782 2314 9 0.21308308031404266 2314 10-11 0.193877048106188 2314 12-13 0.17224774616376948 2314 14-15 0.12672898909290353 2314 16-17 0.10682941052108674 2314 18-19 0.1194255269904474 2314 20-21 0.2039289803980111 2314 22-23 0.15066324422679855 2314 24-25 0.17599935189956284 2314 26-27 0.17153048555945816 2314 28-29 0.20828349958623704 2314 30-31 0.24038438283754004 2314 32-33 0.09786608831331023 2314 34-35 0.1871408627381257 2314 36-37 0.17066462944934813 2314 38-39 0.18410264959712208 2314 40-41 0.2260150209064733 2314 42-43 0.19641779530353887 2314 44-45 0.27773256156886106 2314 46-47 0.2443581489512141 2314 48-49 0.2523743522297295 2314 50-51 0.24523377591432194 2314 52-53 0.30042443154734855 2314 54-55 0.2503123277599997 2314 56-57 0.16805713122963084 2314 58-59 0.19315374615842984 2314 60-61 0.1639912595969264 2314 62-63 0.17883133946502738 2314 64-65 0.1354622655231168 2314 66-67 0.13808443752374444 2314 68-69 0.05153056467396411 2314 70-71 -0.4332854586306212 2314 72-73 -0.2884272488777597 2314 74-75 -0.1496914496517796 2314 76-77 -0.22184162689496745 2314 78-79 -0.05756005439661038 2314 80-81 -0.0664710504773467 2314 82-83 -0.06974547338741743 2314 84-85 -0.03187772412113077 2314 86-87 -0.042563624015901524 2314 88-89 -0.022819316636134346 2314 90-91 0.03565316768534288 2314 92-93 -0.01845698503288773 2314 94-95 -0.024949585089387938 2314 96-97 -0.009629437683713604 2314 98-99 -0.04751887360651352 2314 100-101 -0.05982018344479556 2315 1 0.8358697838511233 2315 2 1.1554446838452321 2315 3 1.8914075833781325 2315 4 1.1675797621586668 2315 5 1.56149635208142 2315 6 0.6593821112345353 2315 7 0.3156604738210689 2315 8 0.1796565279470954 2315 9 0.13750137990931677 2315 10-11 0.07634284874889374 2315 12-13 0.09608925209121111 2315 14-15 -0.019670165990014254 2315 16-17 0.04901104461003314 2315 18-19 0.12319292759357126 2315 20-21 0.10477664821443256 2315 22-23 0.11321290648767501 2315 24-25 0.028775990851933386 2315 26-27 0.01554496664542171 2315 28-29 0.07529635209365892 2315 30-31 0.10640627874506237 2315 32-33 0.0024882402726476016 2315 34-35 0.008615057673928561 2315 36-37 -0.017181667054217087 2315 38-39 -0.05547186099015278 2315 40-41 0.024855382525068137 2315 42-43 -0.11344826460938151 2315 44-45 0.04076425361015623 2315 46-47 -0.08583144060276027 2315 48-49 -0.03349852977152068 2315 50-51 0.006685487180618566 2315 52-53 -0.0691258151966494 2315 54-55 -0.09315060356979643 2315 56-57 -0.06002417002027727 2315 58-59 0.0030210682669462585 2315 60-61 -0.04482793581563271 2315 62-63 -0.07011635955023365 2315 64-65 -0.08027794876689143 2315 66-67 -0.043387164084599306 2315 68-69 -0.12326063232600148 2315 70-71 -0.10064329257463811 2315 72-73 -0.07210568693891517 2315 74-75 -0.0581406910170692 2315 76-77 -0.11763679167716035 2315 78-79 -0.04647449935121983 2315 80-81 -0.03481076044774767 2315 82-83 -0.04477993332931263 2315 84-85 -0.0034876161926078453 2315 86-87 -0.07240155166924112 2315 88-89 -0.04364911589719611 2315 90-91 -0.009148783660247517 2315 92-93 -0.03692883757915055 2315 94-95 -0.07593544213490588 2315 96-97 -0.05998127980571866 2315 98-99 -0.023555314012089212 2315 100-101 -0.10496141218813904 2312 1 -3.4278808937785037 2312 2 -1.2651695406788832 2312 3 -2.6870817556714215 2312 4 -2.1166692240637417 2312 5 -0.05640685881364149 2312 6 -0.19926660546227026 2312 7 -0.20464065466617143 2312 8 -0.10720073751358683 2312 9 -0.1526243540977248 2312 10-11 -0.1973959374682721 2312 12-13 -0.0807876040370985 2312 14-15 -0.09227356707737755 2312 16-17 -0.15571866187520556 2312 18-19 -0.2037505625768503 2312 20-21 -0.1643121344124978 2312 22-23 -0.2998936538429149 2312 24-25 -0.2190278895212856 2312 26-27 -0.2961056316322228 2312 28-29 -0.2238526094390494 2312 30-31 -0.1883207798129618 2312 32-33 -0.22239560002859804 2312 34-35 -0.3303286863766104 2312 36-37 -0.268566029508186 2312 38-39 -0.2696425057950904 2312 40-41 -0.3498772571682238 2312 42-43 -0.29106316381164987 2312 44-45 -0.3310365690964048 2312 46-47 -0.301196497576548 2312 48-49 -0.3062626404396198 2312 50-51 -0.2424163233112182 2312 52-53 -0.24649471950339574 2312 54-55 -0.2986313837380905 2312 56-57 -0.2174286353512187 2312 58-59 -0.37016305424281626 2312 60-61 -0.36451893810749425 2312 62-63 -0.31737953752040227 2312 64-65 -0.2616891138423654 2312 66-67 -0.12220660070757106 2312 68-69 -0.020033845964221086 2312 70-71 0.6401187631370604 2312 72-73 0.24022257307775874 2312 74-75 0.11896187622224375 2312 76-77 0.17975101012193662 2312 78-79 0.15985864788977722 2312 80-81 0.1215264502647031 2312 82-83 0.0919176548399605 2312 84-85 0.09491238265365709 2312 86-87 0.13211630195556623 2312 88-89 0.1652871476757909 2312 90-91 0.18678122126169328 2312 92-93 0.21022842855765944 2312 94-95 0.27070868103790247 2312 96-97 0.2627458952818884 2312 98-99 0.2546776617065625 2312 100-101 0.3142169361342333 2313 1 -1.4594618793200596 2313 2 -0.12842508687335652 2313 3 -0.9783421742152179 2313 4 0.0648378700112957 2313 5 1.0878031409643398 2313 6 0.2523569735829412 2313 7 0.08840962356568838 2313 8 -0.19886425105752892 2313 9 -0.24734858680888294 2313 10-11 -0.1851248572509263 2313 12-13 -0.3554314351914414 2313 14-15 -0.40461165327741355 2313 16-17 -0.24675972624181242 2313 18-19 -0.27314427898839 2313 20-21 -0.35845692541583674 2313 22-23 -0.277925989021675 2313 24-25 -0.3603214763272433 2313 26-27 -0.25108060160400925 2313 28-29 -0.2939722664912239 2313 30-31 -0.26192039125513844 2313 32-33 -0.24004976615557183 2313 34-35 -0.44905696967695263 2313 36-37 -0.37286570035695377 2313 38-39 -0.42666930549463444 2313 40-41 -0.2777946619061993 2313 42-43 -0.25501852215767684 2313 44-45 -0.3157546358607064 2313 46-47 -0.3178059065941383 2313 48-49 -0.27787979608416435 2313 50-51 -0.2608668735732991 2313 52-53 -0.2151798469289119 2313 54-55 -0.0794671980553403 2313 56-57 -0.1330829489159413 2313 58-59 -0.2071116490457996 2313 60-61 -0.1872956649315256 2313 62-63 -0.2112058866640112 2313 64-65 -0.15302329963678574 2313 66-67 -0.08146797723058796 2313 68-69 -0.010653054152406582 2313 70-71 1.5685571163123804 2313 72-73 0.20802141674011665 2313 74-75 -0.17632858685439246 2313 76-77 -0.17460811438238366 2313 78-79 -0.12441517179825468 2313 80-81 -0.11098473459317404 2313 82-83 -0.07521148072271444 2313 84-85 -0.11235563910656055 2313 86-87 -0.1490704844060744 2313 88-89 -0.15065873723296974 2313 90-91 -0.13298906874635819 2313 92-93 -0.1441295060890333 2313 94-95 -0.16800361554449594 2313 96-97 -0.19424971772609467 2313 98-99 -0.2085967744584778 2313 100-101 -0.028285704892073937 2316 1 -2.4527121461815717 2316 2 -4.577395147124058 2316 3 1.5177922304601523 2316 4 3.0426458509089116 2316 5 -3.3926836907834073 2316 6 -1.3590230725395713 2316 7 -0.9051541082923151 2316 8 -0.6817334954162746 2316 9 -0.9033173384011306 2316 10-11 -0.7776237722866313 2316 12-13 -0.6530459437460934 2316 14-15 -0.6211583808837986 2316 16-17 -0.7861014210293433 2316 18-19 -0.6024955585068952 2316 20-21 -1.01531354291253 2316 22-23 -0.6822298429948717 2316 24-25 -0.5827799034947247 2316 26-27 -0.6554545349582241 2316 28-29 -0.9418477011276281 2316 30-31 -0.9993854326596932 2316 32-33 -0.6690989668941967 2316 34-35 -0.8036692537011518 2316 36-37 -1.0445885852821704 2316 38-39 -0.7662061908574316 2316 40-41 -0.919565083025006 2316 42-43 -0.7866319474276295 2316 44-45 -0.8276173153360489 2316 46-47 -0.8491729118616362 2316 48-49 -0.7450390317978162 2316 50-51 -0.7392280296282365 2316 52-53 -0.8911517454796254 2316 54-55 -0.7357090594341358 2316 56-57 -0.8233329630856758 2316 58-59 -0.7097619894796594 2316 60-61 -0.5967357146291477 2316 62-63 -0.7906505462365203 2316 64-65 -0.47188345541674437 2316 66-67 -0.5166704574718324 2316 68-69 -0.03843193527887934 2316 70-71 2.084634266550154 2316 72-73 0.9779728038947404 2316 74-75 0.604302513326541 2316 76-77 0.6234044395005256 2316 78-79 0.47391633077401707 2316 80-81 0.4675706273570981 2316 82-83 0.5189543171049351 2316 84-85 0.45993011092752667 2316 86-87 0.4583475348556867 2316 88-89 0.3872241973823414 2316 90-91 0.3083371335040468 2316 92-93 0.4463323565857422 2316 94-95 0.31856798266687036 2316 96-97 0.4219806207604577 2316 98-99 0.431345885799189 2316 100-101 0.46347852864155925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 592.0 3 21.0 4 41.0 5 77.0 6 148.0 7 353.0 8 589.0 9 854.0 10 1319.0 11 1699.0 12 2333.0 13 2871.0 14 3249.0 15 3342.0 16 3697.0 17 4123.0 18 5205.0 19 7147.0 20 10319.0 21 14136.0 22 19131.0 23 25946.0 24 37076.0 25 56438.0 26 91388.0 27 128338.0 28 63491.0 29 33238.0 30 38870.0 31 46235.0 32 55737.0 33 67975.0 34 86506.0 35 117235.0 36 162136.0 37 172892.0 38 60129.0 39 2334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.61527507261555 19.296561545213777 11.184113864202322 30.904049517968346 2 16.771637109431214 44.84301148509003 15.759430198485683 22.625921206993073 3 12.143513219651389 12.935810716624232 45.48420566430547 29.43647039941891 4 15.41294206385507 13.14272710626713 9.889088907459051 61.555241922418745 5 44.66879243884135 15.73676691751984 14.440960093550052 25.15348055008876 6 46.10913118496696 19.510250826922643 13.473150443411367 20.907467544699028 7 9.573466143262936 55.979008596981636 16.432817715357782 18.014707544397645 8 10.58212340172241 55.25998146487745 15.56031072701381 18.597584406386332 9 38.133302190308996 26.538226806609355 16.325072897280762 19.00339810580089 10-11 23.69037303817783 40.27757476209492 17.530496304277392 18.501555895449854 12-13 9.017638504833448 41.99877939436864 16.4701516715516 32.51343042924631 14-15 10.063780411539998 38.8830328282638 16.862026356040115 34.191160404156086 16-17 24.06856194787782 24.474903528722123 17.016316577237113 34.440217946162946 18-19 11.362215910696303 23.754833007648045 31.107220719687252 33.7757303619684 20-21 26.125820329864908 23.41859992013321 30.896354005771503 19.55922574423038 22-23 8.8990438589221 52.5766457455866 17.309996157352643 21.214314238138652 24-25 9.202637268578778 24.754286540941443 31.163763890547553 34.879312299932224 26-27 10.162634398474998 23.7079663353953 18.264969371840177 47.86442989428952 28-29 23.84745704605877 38.721480040973276 16.154908825416392 21.27615408755156 30-31 9.050564718469571 23.625161052131915 32.00552286375178 35.31875136564673 32-33 9.279854731492488 39.00060089022169 16.67713358411239 35.042410794173435 34-35 10.557714513914137 37.609552747130614 17.64636073713934 34.18637200181591 36-37 24.76804481026163 38.74702756789502 16.894867073137654 19.590060548705694 38-39 23.68935491612226 23.308929818011535 31.89196499847988 21.109750267386325 40-41 9.40348904976946 37.08966067527395 32.54674563435539 20.9601046406012 42-43 10.250398959615794 22.933396724839174 32.451917643282634 34.3642866722624 44-45 24.074828299329777 23.24609870532665 18.338336128292585 34.34073686705099 46-47 10.570455763226494 36.782813678699625 31.572664606285596 21.074065951788292 48-49 24.887589546910995 23.156986589861493 31.051506309489945 20.903917553737564 50-51 9.780183007481737 22.595153414413744 17.91215309259431 49.712510485510215 52-53 23.559007240753157 22.376225314758027 33.46007790779153 20.604689536697283 54-55 9.367213453193884 23.17977048076075 32.79728362300135 34.655732443044016 56-57 10.203886348053436 22.398452392613073 31.633878587412696 35.763782671920794 58-59 23.898554109749025 22.52104037793567 33.36404939685506 20.21635611546025 60-61 10.300552233081692 24.433205717183423 31.97383580088867 33.29240624884622 62-63 23.750048033779613 25.321788649862754 31.04884677488018 19.87931654147745 64-65 9.188464219112022 54.190329082449566 16.894079529828396 19.727127168610018 66-67 9.195304435620589 55.036731187980806 16.593267079060585 19.174697297338025 68-69 9.205400803188644 54.23346719810731 16.682928850747057 19.878203147956995 70-71 9.341739438370718 53.10135547501903 17.24406838405377 20.312836702556492 72-73 9.93102071262272 50.98368005063253 18.148484414674392 20.936814822070357 74-75 10.723660912741767 46.31546627888578 19.254940815696084 23.705931992676366 76-77 11.356302750852283 42.3528099818373 20.77170943748373 25.51917782982669 78-79 11.900995149901682 41.209371054562006 21.102919817208598 25.786713978327715 80-81 12.511221962987396 38.70722986385211 21.476021071484137 27.305527101676354 82-83 13.186995361945344 35.898618379657364 22.016404622230258 28.89798163616703 84-85 13.709058852781396 35.67528876364705 22.169475817692756 28.4461765658788 86-87 14.34599648887516 33.89019070079339 22.239359257389562 29.524453552941885 88-89 14.378357607311578 33.72616240082579 22.197843596717927 29.6976363951447 90-91 14.883999014456892 33.30966171308188 22.25530388709353 29.551035385367697 92-93 15.137876508492315 32.78888021089411 22.013108921141892 30.06013435947168 94-95 15.556731790749016 32.512752315006665 21.787885865838867 30.142630028405453 96-97 15.723489373597365 32.767174544403794 21.865905358654512 29.643430723344334 98-99 16.12114156979975 31.74586490808709 21.39597613786842 30.73701738424474 100-101 16.70950110872263 31.429894296501743 21.058421356793197 30.802183237982433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 1.5 9 1.5 10 1.0 11 2.0 12 2.0 13 7.0 14 10.0 15 12.5 16 23.0 17 36.0 18 56.5 19 83.5 20 109.0 21 140.5 22 188.5 23 257.0 24 344.5 25 464.0 26 649.5 27 938.0 28 1448.0 29 2286.5 30 3654.5 31 5835.0 32 9149.0 33 14266.5 34 20930.0 35 29035.0 36 38368.0 37 48328.5 38 58701.5 39 69158.0 40 78616.5 41 86481.5 42 92467.5 43 95539.5 44 94945.0 45 90446.0 46 83222.0 47 73895.5 48 63234.5 49 54043.5 50 47075.5 51 39485.5 52 29431.5 53 20351.0 54 14459.5 55 10543.5 56 7980.5 57 6168.5 58 4941.5 59 4079.5 60 3478.0 61 3048.0 62 2699.5 63 2388.0 64 2136.5 65 1877.0 66 1663.5 67 1455.0 68 1236.0 69 1078.5 70 907.0 71 724.0 72 608.0 73 501.5 74 373.0 75 273.0 76 203.0 77 149.0 78 115.0 79 84.5 80 54.5 81 41.5 82 35.0 83 23.5 84 17.5 85 16.0 86 14.0 87 11.5 88 15.0 89 23.0 90 20.0 91 9.5 92 7.0 93 5.5 94 5.0 95 5.5 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0011301903994092872 2 8.288062929001439E-4 3 0.004294723517755291 4 0.008363408955628725 5 4.520761597637149E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.1301903994092872E-4 18-19 6.404412263319294E-4 20-21 0.0 22-23 0.0 24-25 2.6371109319550033E-4 26-27 0.0 28-29 2.6371109319550033E-4 30-31 0.0 32-33 9.418253328410726E-4 34-35 0.003955666397932506 36-37 0.0010548443727820013 38-39 7.91133279586501E-4 40-41 3.767301331364291E-5 42-43 6.027682130182865E-4 44-45 0.008928504155333368 46-47 0.020418773215994456 48-49 0.0032022061316596467 50-51 0.028556144091741325 52-53 0.0 54-55 7.534602662728582E-5 56-57 0.0 58-59 0.0 60-61 0.0039933394112461485 62-63 0.0014315745059184303 64-65 0.0026371109319550034 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0029761680517777895 78-79 0.009305234288469797 80-81 0.0010171713594683584 82-83 0.010925173860956441 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0017329586124275734 92-93 0.007421583622787653 94-95 0.0 96-97 0.0015445935458593592 98-99 1.8836506656821453E-4 100-101 5.274221863910007E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1327210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.41044120184295 #Duplication Level Percentage of deduplicated Percentage of total 1 93.65301361143085 57.51272885760172 2 4.260863897854645 5.233230637365162 3 0.9462392768533929 1.7432691442223904 4 0.394807832571265 0.9698129275257891 5 0.17918980921142325 0.5502062621273782 6 0.15490711119537617 0.5707748426286597 7 0.08679476895048499 0.3731073538682912 8 0.05767694111215085 0.28335731206959186 9 0.036545218401060536 0.2019832187244153 >10 0.19709401707926236 2.2796818444427354 >50 0.015634883367394083 0.6517340044788709 >100 0.012981925627990987 1.5914507943681095 >500 0.0016779614255265616 0.7208145768418767 >1k 0.0018009238720630915 2.143653970706958 >5k 2.572736823824027E-4 1.1705694216407607 >10k+ 5.145473647648053E-4 24.003624831387278 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 260270 19.610310350283676 TruSeq Adapter, Index 5 (100% over 50bp) GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 20096 1.5141537511019356 TruSeq Adapter, Index 5 (98% over 50bp) CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT 12847 0.9679704040807409 TruSeq Adapter, Index 5 (100% over 50bp) GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 10644 0.8019831074208301 TruSeq Adapter, Index 5 (98% over 50bp) GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 9644 0.7266370807935444 TruSeq Adapter, Index 5 (98% over 50bp) GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 5174 0.38984034176957677 TruSeq Adapter, Index 5 (98% over 50bp) ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC 3298 0.2484911958167886 No Hit CGCAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT 3138 0.2364358315564229 TruSeq Adapter, Index 5 (98% over 50bp) CGTAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT 3012 0.22694223220138485 TruSeq Adapter, Index 5 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC 2654 0.19996835466881657 TruSeq Adapter, Index 5 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACTCGTATGCC 1867 0.1406710317131426 TruSeq Adapter, Index 5 (97% over 40bp) AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA 1793 0.13509542574272346 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC 1750 0.13185554659775017 TruSeq Adapter, Index 5 (98% over 50bp) TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 1686 0.12703340089360388 TruSeq Adapter, Index 5 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC 1506 0.11347111610069242 TruSeq Adapter, Index 5 (98% over 50bp) GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT 1462 0.11015589092909185 TruSeq Adapter, Index 5 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.7673013313642905E-4 0.0 0.0 2 3.7673013313642905E-4 0.0 0.0 3 4.520761597637149E-4 0.0 0.0 4 4.520761597637149E-4 0.0 0.0 5 8.288062929001439E-4 0.0 0.0 6 0.0010548443727820013 0.0 0.0 7 0.0012808824526638588 0.0 0.0 8 0.0026371109319550034 0.0 0.0 9 0.0030138410650914324 0.0 0.0 10-11 0.005198875837282721 0.0 0.0 12-13 0.010360078661251798 0.0 0.0 14-15 0.01891185268344874 0.0 0.0 16-17 0.0230935571612631 0.0 0.0 18-19 0.024675823720436103 0.0 0.0 20-21 0.02712456958582289 0.0 0.0 22-23 0.03789905139352476 0.0 0.0 24-25 0.05884524679591022 0.0 0.0 26-27 0.07432885526781746 0.0 0.0 28-29 0.07756873441279075 7.53460266272858E-5 0.0 30-31 0.08020584534474576 7.53460266272858E-5 0.0 32-33 0.08461358790244197 7.53460266272858E-5 0.0 34-35 0.08864460032700175 7.53460266272858E-5 0.0 36-37 0.0931653619246389 7.53460266272858E-5 0.0 38-39 0.09768612352227604 7.53460266272858E-5 0.0 40-41 0.10416588181222264 7.53460266272858E-5 0.0 42-43 0.11162513844832393 7.53460266272858E-5 0.0 44-45 0.12326609956223959 7.53460266272858E-5 0.0 46-47 0.13422894643650968 7.53460266272858E-5 0.0 48-49 0.14869538354894854 7.53460266272858E-5 0.0 50-51 0.16372691586109206 7.53460266272858E-5 0.0 52-53 0.18199832731820886 7.53460266272858E-5 0.0 54-55 0.20531792255935383 7.53460266272858E-5 0.0 56-57 0.2336480285712133 7.53460266272858E-5 0.0 58-59 0.26958808327242867 7.53460266272858E-5 0.0 60-61 0.31140512805057224 7.53460266272858E-5 0.0 62-63 0.3640343276497314 7.53460266272858E-5 0.0 64-65 0.42958537081547005 7.53460266272858E-5 0.0 66-67 0.5079075654945336 7.53460266272858E-5 0.0 68-69 0.60725130160261 7.53460266272858E-5 0.0 70-71 0.7337572803098229 7.53460266272858E-5 0.0 72-73 0.8927373964933959 7.53460266272858E-5 0.0 74-75 1.0970381476932816 7.53460266272858E-5 0.0 76-77 1.338258451940537 7.53460266272858E-5 0.0 78-79 1.631316822507365 1.506920532545716E-4 0.0 80-81 1.9704869613700922 1.506920532545716E-4 0.0 82-83 2.3792768288364314 1.506920532545716E-4 0.0 84-85 2.8657107767421883 1.506920532545716E-4 0.0 86-87 3.449265752970517 1.506920532545716E-4 0.0 88-89 4.140603220289178 1.506920532545716E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGAAG 2505 0.0 89.122536 3 ACCGGAA 2490 0.0 88.324066 2 GACCGGA 2320 0.0 87.42533 1 TCTGAAG 2040 0.0 84.057106 3 CTGAAGA 2275 0.0 82.052574 4 GAAGAGC 47065 0.0 79.512924 6 TTGGAAG 1510 0.0 79.27222 3 TGAAGAG 2355 0.0 79.26223 5 CATCGGA 1385 0.0 79.22452 1 GAGCACA 47220 0.0 79.11111 9 AGAGCAC 47325 0.0 78.95565 8 AAGAGCA 47750 0.0 78.32253 7 ACGGAAG 1460 0.0 78.08288 3 GGAAGAG 45840 0.0 77.52429 5 CGGAAGA 44420 0.0 76.893875 4 TCGGAAG 39665 0.0 76.06772 3 TGGAAGA 1810 0.0 75.3155 4 ATTGGAA 1605 0.0 74.876045 2 ATCGGAA 39990 0.0 74.36862 2 TCTCTGA 1000 0.0 73.62564 1 >>END_MODULE