##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_ATCACG_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16000000
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.8101669375	31.0	28.0	31.0	16.0	34.0
2	30.2108186875	31.0	30.0	33.0	26.0	34.0
3	27.3789200625	31.0	25.0	31.0	16.0	34.0
4	31.9573125	35.0	32.0	37.0	19.0	37.0
5	32.4731265	35.0	32.0	37.0	22.0	37.0
6	34.0134835	35.0	33.0	37.0	30.0	37.0
7	34.6456393125	35.0	35.0	37.0	32.0	37.0
8	34.865325625	35.0	35.0	37.0	32.0	37.0
9	36.520205625	38.0	35.0	39.0	32.0	39.0
10-11	36.698124968749994	39.0	35.0	39.0	32.0	39.0
12-13	36.68514521875	39.0	35.0	39.0	32.5	39.0
14-15	37.78594503125	40.0	37.0	41.0	33.0	41.0
16-17	37.732221281250006	40.0	37.0	41.0	32.0	41.0
18-19	37.765170312500004	39.5	37.0	41.0	32.5	41.0
20-21	37.768039343750004	40.0	37.0	41.0	32.0	41.0
22-23	37.79691096875	40.0	37.0	41.0	32.5	41.0
24-25	37.7443058125	40.0	37.0	41.0	32.0	41.0
26-27	37.431472906249994	39.5	36.5	41.0	31.5	41.0
28-29	37.303304624999996	39.0	36.0	40.5	31.0	41.0
30-31	37.237034406250004	39.0	36.0	40.5	31.0	41.0
32-33	37.08813728125	39.0	36.0	40.0	31.0	41.0
34-35	36.58090046875	39.0	35.5	40.0	30.0	41.0
36-37	36.411061593750006	39.0	35.0	40.0	30.0	41.0
38-39	36.17897753125	39.0	35.0	40.0	29.5	41.0
40-41	36.42206446875	39.0	35.0	40.0	30.0	41.0
42-43	36.491859812499996	39.0	35.0	40.0	30.0	41.0
44-45	36.35545934375	39.0	35.0	40.0	30.0	41.0
46-47	36.215784437500005	39.0	35.0	40.0	30.0	41.0
48-49	36.159247	39.0	35.0	40.0	29.0	41.0
50-51	35.930453156249996	38.5	35.0	40.0	28.0	41.0
52-53	35.609263375	38.0	34.0	40.0	28.0	41.0
54-55	35.514854656249994	38.0	34.0	40.0	28.0	41.0
56-57	35.30718475	38.0	34.0	40.0	28.0	41.0
58-59	34.985197031249996	37.5	34.0	40.0	27.5	41.0
60-61	34.6315781875	37.0	33.0	39.5	26.0	41.0
62-63	34.24270746875	36.5	33.0	39.0	26.0	40.0
64-65	33.621231375	36.0	32.0	39.0	25.5	40.0
66-67	33.34253596875	36.0	33.0	39.0	25.5	40.0
68-69	32.309784875000005	35.0	32.0	38.0	19.5	40.0
70-71	29.9182389375	35.0	29.5	37.0	2.0	40.0
72-73	29.1852625	35.0	29.0	37.0	2.0	39.0
74-75	28.82108528125	34.5	29.0	36.0	2.0	39.0
76-77	26.214734125	31.5	24.0	34.0	2.0	35.5
78-79	28.12773653125	34.0	28.5	35.0	2.0	37.0
80-81	28.254089125	34.0	29.0	35.0	2.0	37.0
82-83	28.09334825	34.0	29.0	35.0	2.0	37.0
84-85	27.907346968749998	34.0	29.0	35.0	2.0	36.0
86-87	27.71624140625	34.0	29.0	35.0	2.0	36.0
88-89	27.492898968749998	34.0	27.5	35.0	2.0	36.0
90-91	27.32144978125	34.0	27.0	35.0	2.0	36.0
92-93	27.1249724375	34.0	27.0	35.0	2.0	35.0
94-95	26.9100798125	34.0	26.5	35.0	2.0	35.0
96-97	26.696114125	34.0	26.0	35.0	2.0	35.0
98-99	26.39516715625	34.0	25.0	35.0	2.0	35.0
100-101	25.0650005	32.5	20.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1307	1	2.4688009739087846
1307	2	1.4453745671225384
1307	3	2.7893267678876263
1307	4	2.3533511980147956
1307	5	3.07429962637309
1307	6	1.4864209020430863
1307	7	0.7730381086477038
1307	8	0.5170099487427819
1307	9	0.6150730421560766
1307	10-11	0.49041275129790307
1307	12-13	0.4971485739360375
1307	14-15	0.4718818762453836
1307	16-17	0.49905406353622794
1307	18-19	0.3855091718200967
1307	20-21	0.622206559500718
1307	22-23	0.3508071280733418
1307	24-25	0.4646117491998254
1307	26-27	0.46104864326224515
1307	28-29	0.6344243877621096
1307	30-31	0.6284935380315986
1307	32-33	0.5684350830447897
1307	34-35	0.7814183740951535
1307	36-37	0.8358314710778458
1307	38-39	0.877608114324552
1307	40-41	0.7123254233246428
1307	42-43	0.5536779503564162
1307	44-45	0.5513229499461616
1307	46-47	0.5082360358213762
1307	48-49	0.5065041170483724
1307	50-51	0.41265483359111954
1307	52-53	0.4137733162454822
1307	54-55	0.26604518752609607
1307	56-57	0.2024683187080356
1307	58-59	0.0921914315314396
1307	60-61	0.07906137502527599
1307	62-63	0.09539573000784429
1307	64-65	-0.38823376334785564
1307	66-67	-0.19113074840859667
1307	68-69	-0.5786322886964044
1307	70-71	0.0353476358671152
1307	72-73	0.2965082868917932
1307	74-75	0.33140827215037305
1307	76-77	-0.408092793325455
1307	78-79	0.12593493460694205
1307	80-81	0.2489331798885992
1307	82-83	0.20538671852848722
1307	84-85	0.19064474657539776
1307	86-87	0.1903861743103228
1307	88-89	0.14784304336407672
1307	90-91	0.07831445662082714
1307	92-93	0.08927089831529145
1307	94-95	0.07500030018974613
1307	96-97	0.027380841364973207
1307	98-99	0.003596384706330724
1307	100-101	-0.48438459643030285
1306	1	2.6357410470306526
1306	2	1.6273390241359174
1306	3	3.600226264546695
1306	4	2.747898113415328
1306	5	3.244509630652118
1306	6	1.6566614820813257
1306	7	0.8448516018554884
1306	8	0.5629823305211161
1306	9	0.6342720943228031
1306	10-11	0.570180231611694
1306	12-13	0.5543310237166779
1306	14-15	0.49090953330932763
1306	16-17	0.6278543039204862
1306	18-19	0.5807493038133558
1306	20-21	0.7751493278911497
1306	22-23	0.6144493323992677
1306	24-25	0.6213022778175272
1306	26-27	0.6744369126803278
1306	28-29	0.7296691901732189
1306	30-31	0.7672675028598022
1306	32-33	0.7267798350744386
1306	34-35	1.0462810875989632
1306	36-37	1.211621096038911
1306	38-39	1.1681415677190259
1306	40-41	0.945076228394143
1306	42-43	0.7926033271295054
1306	44-45	0.9127918419602565
1306	46-47	0.7330725541797776
1306	48-49	0.8180765161556209
1306	50-51	0.8157605428230355
1306	52-53	0.9343429450142438
1306	54-55	0.895523846373969
1306	56-57	0.7374179999855528
1306	58-59	0.6817314052393613
1306	60-61	0.6737490675923539
1306	62-63	0.6868092758600781
1306	64-65	0.22112036361401977
1306	66-67	0.4676433552779855
1306	68-69	0.26423257101019004
1306	70-71	0.12859234110994677
1306	72-73	0.2789443798988387
1306	74-75	0.259469308343828
1306	76-77	0.25322218235530514
1306	78-79	0.24521602696978562
1306	80-81	0.2595420973635072
1306	82-83	0.21389479879372786
1306	84-85	0.27096702042679155
1306	86-87	0.2930774591172032
1306	88-89	0.17440571982224284
1306	90-91	0.22218072640596276
1306	92-93	0.15367768110311886
1306	94-95	0.17371655670361008
1306	96-97	0.2690654427328312
1306	98-99	0.2238504178385945
1306	100-101	-0.024868257809117722
1101	1	1.3696076995160844
1101	2	0.6451415682375767
1101	3	1.4830695054928036
1101	4	-0.9533187054023209
1101	5	0.8699241728387648
1101	6	0.43596643232812937
1101	7	0.28015876632348835
1101	8	0.20797081867347345
1101	9	0.29166038081915957
1101	10-11	0.2867856673171971
1101	12-13	0.22737723969019896
1101	14-15	0.301667237112035
1101	16-17	0.2863052635595409
1101	18-19	0.32658442850583214
1101	20-21	0.36975328896691195
1101	22-23	0.35874012996384863
1101	24-25	0.3659728242323652
1101	26-27	0.3863942578356401
1101	28-29	0.43665628859710637
1101	30-31	0.4211509942466307
1101	32-33	0.33974545942156453
1101	34-35	0.5004724258314184
1101	36-37	0.587325913615679
1101	38-39	0.5513297985612766
1101	40-41	0.45741600611913213
1101	42-43	0.2999218560364909
1101	44-45	0.2918146486762154
1101	46-47	0.29813521225135986
1101	48-49	0.3339608742418818
1101	50-51	0.21025902015414033
1101	52-53	0.3141828728059224
1101	54-55	0.2481268504309142
1101	56-57	0.19611971937185757
1101	58-59	0.11195179132361233
1101	60-61	0.03807958560567215
1101	62-63	0.15931446352942658
1101	64-65	-0.14731170882303957
1101	66-67	-0.07225099590789341
1101	68-69	-0.30851531772295715
1101	70-71	0.08559653162707193
1101	72-73	0.35028575469594614
1101	74-75	0.37917118089251645
1101	76-77	0.05551893542258313
1101	78-79	0.2910448687470115
1101	80-81	0.29946178157369374
1101	82-83	0.2600509190606388
1101	84-85	0.19539633363665487
1101	86-87	0.273000185798697
1101	88-89	0.15245101320071086
1101	90-91	0.27357161799318774
1101	92-93	0.16200827419172015
1101	94-95	0.05109789277578969
1101	96-97	0.12450461673590496
1101	98-99	0.09438186822172057
1101	100-101	-0.20830989734653826
1305	1	0.8757285911292385
1305	2	0.27106432308679373
1305	3	0.7325959519352772
1305	4	-0.7429213264927839
1305	5	1.2964224891694656
1305	6	0.570279826431566
1305	7	0.28402684169446957
1305	8	0.20895898898400844
1305	9	0.20062535012230143
1305	10-11	0.21363734751842145
1305	12-13	0.180831619542424
1305	14-15	0.210354151163898
1305	16-17	0.20713162556485543
1305	18-19	0.2139297599852341
1305	20-21	0.3112332293139417
1305	22-23	0.18075826530694172
1305	24-25	0.2493224050839018
1305	26-27	0.19889100471608856
1305	28-29	0.2672985277423052
1305	30-31	0.24489200167361957
1305	32-33	0.17607362033768936
1305	34-35	0.3843055995791005
1305	36-37	0.486639050552931
1305	38-39	0.48870086239605115
1305	40-41	0.2899686392210157
1305	42-43	0.195260647033777
1305	44-45	0.17694989300969866
1305	46-47	0.1993585314628703
1305	48-49	0.29635419037294497
1305	50-51	0.20635497075272013
1305	52-53	0.2082810949502516
1305	54-55	0.09863478912259183
1305	56-57	0.11722095899565232
1305	58-59	0.1193124769521603
1305	60-61	0.06303915314393294
1305	62-63	0.05995198078257857
1305	64-65	-0.23679924912193684
1305	66-67	-0.1805914575603893
1305	68-69	-0.4788479492409152
1305	70-71	0.05878591310360903
1305	72-73	0.3696191915624851
1305	74-75	0.3226782548590883
1305	76-77	0.042108547779626804
1305	78-79	0.3086159519613574
1305	80-81	0.39085728427718536
1305	82-83	0.39245644262166834
1305	84-85	0.40829054151149435
1305	86-87	0.4061967046794379
1305	88-89	0.34987368883756886
1305	90-91	0.32103647227209464
1305	92-93	0.2845666967360714
1305	94-95	0.20495520916778887
1305	96-97	0.27409313653365075
1305	98-99	0.22763599028404968
1305	100-101	0.012327646091335964
1102	1	2.331068693812526
1102	2	1.2217877801554486
1102	3	2.228993252725406
1102	4	3.0288408912279614
1102	5	3.0729988593244855
1102	6	1.2829485173957451
1102	7	0.5921572581813024
1102	8	0.361524991608853
1102	9	0.23431247961045898
1102	10-11	0.13438223552566342
1102	12-13	0.13419602956704324
1102	14-15	0.21423852781118313
1102	16-17	0.17072161640923866
1102	18-19	0.21243711938898713
1102	20-21	0.30896299239783076
1102	22-23	0.1517336556545601
1102	24-25	0.12306333010909754
1102	26-27	0.15359448735071624
1102	28-29	0.14705736365421984
1102	30-31	0.2742533531990219
1102	32-33	0.2906824600988358
1102	34-35	0.366328013455572
1102	36-37	0.24375200886967008
1102	38-39	0.2667453591022806
1102	40-41	0.2860926438087432
1102	42-43	0.08755032514801542
1102	44-45	0.26521510514987057
1102	46-47	0.15875426123643166
1102	48-49	0.15452710259857838
1102	50-51	0.08926894829595966
1102	52-53	0.19580644507684752
1102	54-55	0.057370038223218955
1102	56-57	0.11459611063381914
1102	58-59	0.05703117428124216
1102	60-61	-0.09439878732848683
1102	62-63	0.04788900232265547
1102	64-65	-0.41504743415668344
1102	66-67	-0.17046260825870263
1102	68-69	-0.36993413732498936
1102	70-71	0.07012353326323151
1102	72-73	0.4686502368895731
1102	74-75	0.5201421740785825
1102	76-77	0.1586815903894312
1102	78-79	0.45928256662167044
1102	80-81	0.534177152984757
1102	82-83	0.5850763551321947
1102	84-85	0.5726012472849185
1102	86-87	0.5687552923894721
1102	88-89	0.5474093128175639
1102	90-91	0.5279316150065121
1102	92-93	0.5677285483303152
1102	94-95	0.5209224835840729
1102	96-97	0.40195004542160717
1102	98-99	0.35896067367793094
1102	100-101	0.15393469553901085
1304	1	2.0836214461311933
1304	2	1.0797978890828652
1304	3	1.4440010816521287
1304	4	2.7608028912782068
1304	5	2.4932765897607823
1304	6	1.0707896505163745
1304	7	0.5164842316377118
1304	8	0.32484955641108115
1304	9	0.18836250436137192
1304	10-11	0.09889354079639645
1304	12-13	0.06731845656360491
1304	14-15	-0.005011744398466078
1304	16-17	-0.00664709288071208
1304	18-19	0.0987395533218276
1304	20-21	0.18190251209782105
1304	22-23	0.050546717136143116
1304	24-25	0.12234995055283804
1304	26-27	0.07911340240269027
1304	28-29	-0.04292730415915713
1304	30-31	0.026100049689254945
1304	32-33	0.04519923769113632
1304	34-35	0.15517552604531204
1304	36-37	0.25977992218206936
1304	38-39	0.2811304840972255
1304	40-41	0.22910050387427106
1304	42-43	0.08062603103780219
1304	44-45	0.09895633892276834
1304	46-47	0.019456841546350745
1304	48-49	-0.0012005339598246678
1304	50-51	-0.024436296409966474
1304	52-53	0.019256159025331954
1304	54-55	0.041710003135982276
1304	56-57	-0.04267637701630633
1304	58-59	-0.1350289062634218
1304	60-61	-0.13831342251705792
1304	62-63	-0.19296143003763433
1304	64-65	-0.1381364322846892
1304	66-67	-0.1530330994994742
1304	68-69	-0.18220549621074866
1304	70-71	0.16081652160748305
1304	72-73	0.4312654885086715
1304	74-75	0.4931169864787215
1304	76-77	0.1287871033846777
1304	78-79	0.47049925618403776
1304	80-81	0.5522679483384749
1304	82-83	0.5208240294676116
1304	84-85	0.5303234445003362
1304	86-87	0.4647964939928144
1304	88-89	0.46628764523428856
1304	90-91	0.5362088392038977
1304	92-93	0.5097557060805826
1304	94-95	0.4967544686934957
1304	96-97	0.4495890932867397
1304	98-99	0.4328092486492672
1304	100-101	0.3401844547170114
1103	1	1.4367403152034868
1103	2	0.7798721515476679
1103	3	-0.04273636693033467
1103	4	-0.06454651568522252
1103	5	1.872059378884451
1103	6	0.8064379744852843
1103	7	0.3670968757310433
1103	8	0.18088837986224604
1103	9	0.14646458384216032
1103	10-11	0.09632773109089499
1103	12-13	-0.030712178272828794
1103	14-15	-0.07845708123431905
1103	16-17	-0.07349225199097731
1103	18-19	0.04381551289448993
1103	20-21	0.07407650003214883
1103	22-23	-0.05967098609912114
1103	24-25	-0.015225483873685164
1103	26-27	-0.054917574065846964
1103	28-29	-0.04213152513811025
1103	30-31	0.05835480129425008
1103	32-33	-0.016317205065952578
1103	34-35	0.030799530093858607
1103	36-37	0.24371848243480088
1103	38-39	0.32251672834226497
1103	40-41	0.18192315689837812
1103	42-43	0.027437470340437642
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1311	1	1.8472922075846085
1311	2	0.9249824748857591
1311	3	3.1010447463310165
1311	4	0.16355447765292652
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1213	24-25	0.8899856507494306
1213	26-27	0.9106081577634555
1213	28-29	1.043292864593134
1213	30-31	1.1025799669597447
1213	32-33	0.9754292673923857
1213	34-35	1.113418949089322
1213	36-37	-1.5693405463245966
1213	38-39	-0.7738626830353965
1213	40-41	0.757064785174208
1213	42-43	0.9765074070113968
1213	44-45	1.0181311701643878
1213	46-47	0.8104644917354946
1213	48-49	0.7466969571483517
1213	50-51	0.9131030607319417
1213	52-53	0.9470809485674536
1213	54-55	0.9089707813344248
1213	56-57	0.8576941501518576
1213	58-59	0.8407721723036516
1213	60-61	0.7843027719591333
1213	62-63	0.7194580810746274
1213	64-65	0.7876375750771842
1213	66-67	0.7564563937702857
1213	68-69	0.5451130213077064
1213	70-71	0.3705017094408767
1213	72-73	0.42425624851347266
1213	74-75	0.5163286017038793
1213	76-77	0.9250550297996014
1213	78-79	0.4075800860646659
1213	80-81	0.31435627257034326
1213	82-83	0.35156754950033786
1213	84-85	0.31280818195913085
1213	86-87	0.2699687067174814
1213	88-89	0.19548143962065723
1213	90-91	0.21809473771263654
1213	92-93	0.2302535750144017
1213	94-95	0.16693730302255005
1213	96-97	0.20766282779089096
1213	98-99	0.3965446276129434
1213	100-101	0.2589379835632357
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4932.0
3	564.0
4	790.0
5	1364.0
6	2741.0
7	6005.0
8	10086.0
9	14663.0
10	19644.0
11	25497.0
12	32476.0
13	40348.0
14	44488.0
15	48814.0
16	54319.0
17	61945.0
18	74482.0
19	93416.0
20	117304.0
21	145986.0
22	181650.0
23	232934.0
24	310642.0
25	442250.0
26	619579.0
27	660323.0
28	301514.0
29	350260.0
30	437769.0
31	547450.0
32	686644.0
33	877022.0
34	1147231.0
35	1537055.0
36	2142071.0
37	2759808.0
38	1781084.0
39	184788.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.686812431836287	23.734469487973993	13.462135951220066	36.116582128969654
2	16.824340545827035	36.38289785374054	22.33227040947872	24.460491190953707
3	11.95146790450736	14.991124083204236	40.814454275184296	32.24295373710411
4	16.421964365070398	18.913460144575343	13.450686585663851	51.21388890469041
5	31.258121506201547	22.16582832066361	16.48896931410503	30.087080859029808
6	29.25908597215724	27.023566383837395	19.238438464414422	24.478909179590943
7	10.2461	43.51356875	27.182018749999997	19.0583125
8	9.843244365202773	45.1194528199658	22.60162641260165	22.435676402229777
9	22.10423125	37.5651625	23.48364375	16.8469625
10-11	15.838624844742158	41.221860854098914	25.93233611231302	17.007178188845913
12-13	8.940603125	44.595971875000004	22.73225625	23.731168750000002
14-15	9.78758767310441	40.81626116067139	22.78792120457329	26.608229961650913
16-17	16.01467214242317	36.92427509757668	22.803623954303447	24.25742880569671
18-19	13.025439733118866	30.340813323456622	29.619835259299855	27.013911684124658
20-21	21.95755625	30.545371875	29.573078125000002	17.92399375
22-23	8.758294571090117	46.58032311690529	23.187170923797275	21.47421138820732
24-25	8.899228125	33.648396874999996	29.146884375	28.305490625
26-27	9.35911366988921	33.422657302832164	26.066525133315643	31.151703893962985
28-29	15.455132017430387	39.982485613506185	22.297252132571714	22.265130236491718
30-31	8.840136308779819	30.50951292395595	32.32346957075897	28.326881196505262
32-33	8.909425	40.09526875	24.182525	26.81278125
34-35	9.230406056716276	40.099295266856025	29.874258412069484	20.796040264358208
36-37	9.56344766116314	40.00598938136372	23.847262837716766	26.583300119756377
38-39	15.34935840134195	33.316923952692605	24.420917769690483	26.912799876274956
40-41	9.135846203880009	39.47337475204197	30.548432523368607	20.842346520709413
42-43	9.445822783283164	32.92013481938292	30.792080281604267	26.841962115729643
44-45	15.667432659417846	33.25609652089948	24.47434188882368	26.60212893085899
46-47	9.660728973417717	39.139438510265265	30.218426011523537	20.981406504793483
48-49	15.994158799047383	32.734830806593095	30.199524041957527	21.071486352401994
50-51	9.250453629348916	32.74732086520595	24.359201367297135	33.643024138148
52-53	15.440029318131257	32.823084847045614	31.17869631630506	20.558189518518073
54-55	9.059743776003144	32.76197131007491	31.056704063330525	27.12158085059142
56-57	9.415103071396459	32.69043887105701	30.570032464881276	27.324425592665257
58-59	15.607618749999999	32.892040625	31.045374999999996	20.454965625
60-61	9.43938616285157	33.47022964580376	30.53473499657249	26.55564919477218
62-63	15.49837526526959	34.18719185344822	30.14368159393035	20.170751287351845
64-65	9.081340011365054	46.705931228173334	23.787100488727322	20.425628271734293
66-67	9.029459445937453	46.75242467247308	23.929283004700423	20.288832876889042
68-69	9.121093957520959	46.48638209387689	24.035783996014974	20.356739952587173
70-71	9.383204297900537	45.00001500000187	24.456228057028508	21.16055264506908
72-73	9.77965	43.1760375	25.0532125	21.9911
74-75	10.112749579358612	41.91617597306895	25.397092907230896	22.573981540341542
76-77	10.308230131873177	40.87928159689718	25.830914140166485	22.98157413106316
78-79	10.587004739916766	40.05866388016048	26.07062034418889	23.283711035733862
80-81	10.92949868424153	39.35014725757034	26.21772894769261	23.502625110495522
82-83	11.10647666369787	38.944839763121294	26.08340755324302	23.865276019937813
84-85	11.320187497181626	38.46351299058017	26.25817109528763	23.958128416950572
86-87	11.470625606680711	38.40302730510858	26.24564741453001	23.8806996736807
88-89	11.878372306584422	37.68282744659044	26.127108446498504	24.31169180032664
90-91	11.961087500000001	37.726309375	26.069874999999996	24.242728125
92-93	12.198272411096333	37.56631489763085	25.996239871944898	24.239172819327916
94-95	12.38417800917801	37.53825316325317	25.746256996256996	24.33131183131183
96-97	12.558466982157299	37.25348459666724	25.74455996475692	24.443488456418542
98-99	12.800320400510639	37.118931033296334	25.567790748666507	24.51295781752652
100-101	13.267750951408813	36.739894444473784	25.26402866081258	24.728325943304817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	9.5
2	12.5
3	18.5
4	35.5
5	50.5
6	75.0
7	127.0
8	220.5
9	459.0
10	960.5
11	1939.0
12	3815.5
13	6873.0
14	11428.0
15	17449.5
16	25164.0
17	35062.0
18	47791.0
19	64890.5
20	87378.5
21	117428.0
22	157069.5
23	206130.5
24	264988.5
25	331078.0
26	400902.0
27	470593.5
28	536903.5
29	593582.0
30	636636.0
31	667537.5
32	686159.0
33	693116.5
34	685822.5
35	669020.5
36	646401.0
37	620904.0
38	601377.5
39	587277.0
40	580380.0
41	579701.5
42	574721.5
43	560308.0
44	535897.5
45	500919.5
46	453589.5
47	398019.5
48	339052.0
49	280767.5
50	228992.5
51	184103.0
52	145654.5
53	115392.0
54	93249.5
55	76488.5
56	63616.5
57	53659.0
58	46433.5
59	41430.0
60	37188.0
61	33325.0
62	30018.0
63	26918.0
64	23952.5
65	21215.5
66	18382.5
67	15442.0
68	12858.5
69	10856.0
70	8895.0
71	7096.0
72	5664.5
73	4414.0
74	3425.0
75	2689.5
76	2056.0
77	1539.0
78	1183.0
79	917.5
80	675.5
81	507.0
82	394.5
83	328.0
84	259.5
85	189.0
86	147.0
87	105.5
88	81.5
89	58.5
90	37.5
91	33.0
92	28.5
93	19.5
94	14.0
95	9.0
96	6.0
97	2.5
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022412500000000002
2	0.0065562500000000004
3	0.0032875
4	0.01839375
5	2.6875E-4
6	3.7500000000000003E-5
7	0.0
8	6.2499999999999995E-6
9	0.0
10-11	1.8750000000000002E-5
12-13	0.0
14-15	6.5625E-5
16-17	1.1875000000000001E-4
18-19	4.25E-4
20-21	0.0
22-23	9.375000000000001E-6
24-25	0.0
26-27	1.2499999999999999E-5
28-29	6.5625E-5
30-31	2.1875E-5
32-33	0.0
34-35	2.6875E-4
36-37	1.0625E-4
38-39	3.4375E-5
40-41	4.0312499999999995E-4
42-43	0.004178125
44-45	0.005853125000000001
46-47	0.005184375000000001
48-49	0.022699999999999998
50-51	0.019903125
52-53	0.002153125
54-55	8.28125E-4
56-57	5.968750000000001E-4
58-59	0.0
60-61	3.5E-4
62-63	2.1875E-5
64-65	4.7500000000000005E-4
66-67	3.46875E-4
68-69	6.875000000000001E-4
70-71	1.2499999999999999E-5
72-73	0.0
74-75	1.8125E-4
76-77	0.0021718749999999998
78-79	2.21875E-4
80-81	0.0030187499999999997
82-83	0.0015625
84-85	4.968749999999999E-4
86-87	1.6874999999999998E-4
88-89	5.625E-5
90-91	0.0
92-93	8.125E-5
94-95	9.999999999999999E-5
96-97	0.0016531249999999999
98-99	1.59375E-4
100-101	0.001090625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.64634831578606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.679213334905	62.648625936275984
2	7.922285475767553	11.193610783563349
3	1.572890482123714	3.3335690658808965
4	0.6860491090762773	1.938674572861495
5	0.32626040748171403	1.152455319430173
6	0.17782080902571284	0.753743448733523
7	0.10761975158660939	0.5322059719372183
8	0.09615652003287657	0.5434485605661167
9	0.08026233896569158	0.5103217039688941
>10	0.33301627455819877	3.8950967689491938
>50	0.011088066043573435	0.5421908724780874
>100	0.006102302641474057	0.8517730207595131
>500	6.868225703821117E-4	0.32088859434255657
>1k	4.398145941051703E-4	0.6366892378415046
>5k	3.6928423190688726E-5	0.1690668408748738
>10k+	7.156220387895436E-5	10.977639301536685
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	1548137	9.67585625	TruSeq Adapter, Index 1 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT	87370	0.5460625	TruSeq Adapter, Index 1 (100% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	25494	0.1593375	TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC	17648	0.11030000000000001	TruSeq Adapter, Index 1 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	17632	0.11019999999999999	TruSeq Adapter, Index 1 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	17239	0.10774375	TruSeq Adapter, Index 1 (98% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	8.0625E-4	0.0	6.25E-6
2	0.00111875	0.0	6.25E-6
3	0.00123125	0.0	6.25E-6
4	0.00138125	0.0	6.25E-6
5	0.001525	0.0	6.25E-6
6	0.00193125	0.0	6.25E-6
7	0.00234375	0.0	6.25E-6
8	0.00288125	0.0	6.25E-6
9	0.00305625	0.0	6.25E-6
10-11	0.0046375	0.0	9.375000000000001E-6
12-13	0.0068000000000000005	0.0	2.1875E-5
14-15	0.010078125	0.0	2.5E-5
16-17	0.01249375	0.0	2.5E-5
18-19	0.013746874999999999	6.25E-6	2.5E-5
20-21	0.015471875	1.25E-5	2.5E-5
22-23	0.0211375	1.25E-5	2.5E-5
24-25	0.032196875	1.25E-5	2.5E-5
26-27	0.037340625	1.25E-5	2.5E-5
28-29	0.039281250000000004	1.25E-5	2.5E-5
30-31	0.041234375000000004	1.25E-5	2.5E-5
32-33	0.0435375	1.25E-5	3.125E-5
34-35	0.045774999999999996	1.25E-5	4.0625E-5
36-37	0.04825	1.25E-5	4.375E-5
38-39	0.05070625	1.25E-5	5.0E-5
40-41	0.053643750000000004	1.25E-5	5.0E-5
42-43	0.057225	1.25E-5	5.0E-5
44-45	0.061496875	1.25E-5	5.0E-5
46-47	0.066871875	1.875E-5	5.0E-5
48-49	0.0737125	1.875E-5	5.0E-5
50-51	0.08179687499999999	1.875E-5	5.0E-5
52-53	0.091978125	1.875E-5	5.0E-5
54-55	0.105421875	1.875E-5	5.0E-5
56-57	0.12198125	1.875E-5	5.0E-5
58-59	0.143853125	1.875E-5	5.0E-5
60-61	0.17175	1.875E-5	5.0E-5
62-63	0.2084375	2.8125E-5	5.0E-5
64-65	0.25655937500000003	3.125E-5	5.625E-5
66-67	0.31763125000000003	3.125E-5	5.625E-5
68-69	0.39726875	3.125E-5	5.9375E-5
70-71	0.500096875	4.375E-5	6.25E-5
72-73	0.628853125	4.375E-5	6.25E-5
74-75	0.789215625	5.0E-5	6.562500000000001E-5
76-77	0.98663125	5.0E-5	6.875E-5
78-79	1.2248375	5.0E-5	6.875E-5
80-81	1.5077875	5.0E-5	7.1875E-5
82-83	1.8469375000000001	5.0E-5	7.5E-5
84-85	2.2416718749999998	5.0E-5	8.125E-5
86-87	2.697115625	5.3125000000000004E-5	9.0625E-5
88-89	3.22166875	5.625E-5	9.687500000000001E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGA	11425	0.0	77.94543	1
CCGGAAG	11990	0.0	77.34133	3
GAGCACA	271200	0.0	72.902794	9
ACCGGAA	12740	0.0	72.82988	2
AGAGCAC	273955	0.0	72.2234	8
AAGAGCA	279020	0.0	70.98213	7
GAAGAGC	279520	0.0	70.889145	6
TCCGAAG	5895	0.0	68.66048	3
GGAAGAG	280750	0.0	68.63855	5
CGGAAGA	277565	0.0	68.06741	4
CGAAGAG	7155	0.0	67.975395	5
TCGGAAG	257890	0.0	67.77875	3
ATCGGAA	264080	0.0	66.21914	2
GATCGGA	266725	0.0	64.45344	1
CCGAAGA	7775	0.0	62.077007	4
AATCATA	160430	0.0	61.14473	5
ATCATAA	171785	0.0	60.036213	6
CATAAAC	172740	0.0	59.12414	8
TCATAAA	173345	0.0	59.079453	7
TAAATCA	156380	0.0	58.178333	3
>>END_MODULE