##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2112_lane1_ATCACG_L001_R1_002.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.5360111875 31.0 28.0 33.0 16.0 34.0 2 30.1040033125 31.0 30.0 33.0 25.0 34.0 3 26.77101375 31.0 25.0 31.0 16.0 34.0 4 30.7567555 35.0 28.0 37.0 19.0 37.0 5 32.8406999375 35.0 32.0 37.0 26.0 37.0 6 34.1997778125 35.0 35.0 37.0 30.0 37.0 7 34.8256354375 35.0 35.0 37.0 32.0 37.0 8 35.01934225 36.0 35.0 37.0 32.0 37.0 9 36.7342170625 39.0 37.0 39.0 32.0 39.0 10-11 36.8366270625 39.0 37.0 39.0 32.5 39.0 12-13 36.90628890625 39.0 37.0 39.0 33.0 39.0 14-15 38.15268340625 40.0 37.0 41.0 33.0 41.0 16-17 38.12050859375 40.0 37.0 41.0 33.0 41.0 18-19 38.17584640625 40.0 37.5 41.0 33.0 41.0 20-21 38.20209478125 40.0 38.0 41.0 33.0 41.0 22-23 38.1956868125 40.0 38.0 41.0 33.0 41.0 24-25 38.13837759375 40.0 38.0 41.0 33.0 41.0 26-27 37.89015471875 40.0 37.0 41.0 33.0 41.0 28-29 37.705548218749996 40.0 37.0 41.0 32.5 41.0 30-31 37.66611321875 40.0 37.0 41.0 32.0 41.0 32-33 37.4369914375 40.0 37.0 41.0 31.5 41.0 34-35 37.11199603125 40.0 36.0 41.0 30.5 41.0 36-37 36.742934500000004 39.5 36.0 41.0 30.0 41.0 38-39 36.57320721875 39.0 36.0 41.0 30.0 41.0 40-41 36.9945223125 39.5 36.0 41.0 30.5 41.0 42-43 37.0616075625 40.0 36.0 41.0 31.0 41.0 44-45 36.972875125 40.0 36.0 41.0 30.5 41.0 46-47 36.784920031249996 39.0 36.0 41.0 30.0 41.0 48-49 36.74314971875 39.0 36.0 41.0 30.0 41.0 50-51 36.62064328125 39.0 35.5 41.0 30.0 41.0 52-53 36.3007954375 39.0 35.0 40.5 29.5 41.0 54-55 36.175922656249995 39.0 35.0 40.0 29.0 41.0 56-57 35.975378343749995 39.0 35.0 40.0 29.0 41.0 58-59 35.652664375 38.0 34.5 40.0 28.0 41.0 60-61 35.33879725 38.0 34.0 40.0 28.0 41.0 62-63 34.917301843749996 37.0 34.0 39.5 28.0 41.0 64-65 34.491358875 36.5 34.0 39.0 27.5 41.0 66-67 34.132902656249996 36.0 34.0 39.0 26.5 41.0 68-69 33.187050625 35.5 33.0 39.0 24.0 40.0 70-71 30.48117209375 35.0 30.5 37.5 2.0 40.0 72-73 29.52454665625 35.0 30.0 37.0 2.0 39.5 74-75 29.17315871875 35.0 29.0 37.0 2.0 39.0 76-77 27.25921409375 32.5 26.0 34.5 2.0 36.5 78-79 28.55743259375 34.0 29.0 36.0 2.0 37.5 80-81 28.567458062500002 35.0 29.0 35.5 2.0 37.0 82-83 28.3681411875 34.0 29.0 35.0 2.0 37.0 84-85 28.1621476875 34.0 29.0 35.0 2.0 36.0 86-87 27.97806228125 34.0 29.0 35.0 2.0 36.0 88-89 27.7784013125 34.0 29.0 35.0 2.0 36.0 90-91 27.61095690625 34.0 29.0 35.0 2.0 36.0 92-93 27.439807406249997 34.0 29.0 35.0 2.0 35.5 94-95 27.25973434375 34.0 27.5 35.0 2.0 35.0 96-97 27.0434289375 34.0 27.0 35.0 2.0 35.0 98-99 26.8004119375 34.0 27.0 35.0 2.0 35.0 100-101 25.71422934375 33.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 -0.3524264371206449 2306 2 0.12614728202059666 2306 3 -2.6609014352922067 2306 4 -1.1709643335227078 2306 5 0.5620519715885166 2306 6 0.2361558228095717 2306 7 0.14112231724880786 2306 8 0.18472267630893668 2306 9 0.3678872600410017 2306 10-11 0.5000966728983371 2306 12-13 0.45539048702218565 2306 14-15 0.3762735584075969 2306 16-17 0.4759641047756631 2306 18-19 0.389718760329238 2306 20-21 0.5253560684470173 2306 22-23 0.4762217180811916 2306 24-25 0.49680797876276017 2306 26-27 0.3208249530254932 2306 28-29 0.48992122638811253 2306 30-31 0.4572585784430885 2306 32-33 0.5592359762380781 2306 34-35 0.6267299619120763 2306 36-37 -7.221572341992086 2306 38-39 -4.920610792701922 2306 40-41 -0.7125302301333889 2306 42-43 0.09175656543947497 2306 44-45 0.3085332754233505 2306 46-47 0.32853488873299597 2306 48-49 0.43765734218376906 2306 50-51 0.3847459215668252 2306 52-53 0.4576042536422307 2306 54-55 0.23425050258572355 2306 56-57 0.12546877290638037 2306 58-59 0.10946947675068941 2306 60-61 0.06798961960247851 2306 62-63 0.07388647043062235 2306 64-65 -0.11663717502145943 2306 66-67 -0.010355372804561114 2306 68-69 -0.35408278311783903 2306 70-71 -0.1149677853158444 2306 72-73 0.15606741040494398 2306 74-75 0.17063528536055017 2306 76-77 -0.06830509450210442 2306 78-79 0.15173966996511723 2306 80-81 0.11697998722219083 2306 82-83 0.0394326046934097 2306 84-85 0.10689487440674128 2306 86-87 0.08818562456717416 2306 88-89 0.04216776787110277 2306 90-91 0.060724725196994456 2306 92-93 0.07645227117501108 2306 94-95 0.05115944145791573 2306 96-97 0.0361010938194859 2306 98-99 0.04098434035196874 2306 100-101 -0.27963719496296946 2307 1 -1.7125120561790723 2307 2 -0.7066544771314867 2307 3 1.2020248686342363 2307 4 2.1698883431951543 2307 5 1.8388726814288674 2307 6 0.9633436297910478 2307 7 0.48480860769831935 2307 8 0.27346785401804397 2307 9 0.49504925128702126 2307 10-11 0.4759488117950639 2307 12-13 0.3966729253317567 2307 14-15 0.16908523036967438 2307 16-17 0.27337749031931935 2307 18-19 0.1983040409270913 2307 20-21 0.548377297502249 2307 22-23 0.48547203149443163 2307 24-25 0.47254327064324997 2307 26-27 0.4154498508629558 2307 28-29 0.556201460068543 2307 30-31 0.5597584396226978 2307 32-33 -2.476777835033211 2307 34-35 0.09378617045663162 2307 36-37 0.9151125258732336 2307 38-39 0.8391856847140318 2307 40-41 0.5808716990829339 2307 42-43 0.4646035452377859 2307 44-45 0.5490574021259036 2307 46-47 0.503572466972642 2307 48-49 0.6195137931597969 2307 50-51 0.6141195980919392 2307 52-53 0.607549406108788 2307 54-55 0.5738559673215349 2307 56-57 0.570599250036345 2307 58-59 0.5811146387202655 2307 60-61 0.5321473124886751 2307 62-63 0.49444145731160916 2307 64-65 0.2651668165519325 2307 66-67 0.30142731108683307 2307 68-69 0.15916094151087634 2307 70-71 0.05032528401173764 2307 72-73 -0.07000717894914743 2307 74-75 -0.0665982234790583 2307 76-77 0.03607169512925523 2307 78-79 0.03669583899453599 2307 80-81 -0.06710220861951655 2307 82-83 -0.0943352889710205 2307 84-85 -0.04651980838337977 2307 86-87 -0.03878154850979243 2307 88-89 -0.07177616489735072 2307 90-91 -0.08990038827734992 2307 92-93 -0.12234505041821819 2307 94-95 -0.14129360307249428 2307 96-97 -0.15892973113037812 2307 98-99 -0.1426558579555035 2307 100-101 -0.19632798444004962 2201 1 2.095289550613341 2201 2 1.353384670406335 2201 3 2.202132304946254 2201 4 2.0952364665683803 2201 5 1.9736837473638218 2201 6 0.913643117143458 2201 7 0.450102733228988 2201 8 0.22827886979515455 2201 9 0.3475751539129064 2201 10-11 -3.9511673178566866 2201 12-13 -2.0477127364600136 2201 14-15 -0.4138754134424687 2201 16-17 0.15502447307252254 2201 18-19 0.27230467875197206 2201 20-21 0.4136275393971758 2201 22-23 0.3999387746207006 2201 24-25 0.4630471920839625 2201 26-27 0.39243643468131495 2201 28-29 -1.4925595864837433 2201 30-31 -0.9474267340936464 2201 32-33 0.17959256102995624 2201 34-35 0.4430771431236309 2201 36-37 0.7333657582363813 2201 38-39 0.6738719610897732 2201 40-41 0.5114059373580702 2201 42-43 0.38804350439081503 2201 44-45 0.41868746096232456 2201 46-47 0.2958202793281117 2201 48-49 0.3716745815604128 2201 50-51 0.33191810975877445 2201 52-53 0.33154238331284347 2201 54-55 0.2784677246965259 2201 56-57 0.26708460453259164 2201 58-59 0.25940242545431147 2201 60-61 0.131462404762118 2201 62-63 0.1402368178212896 2201 64-65 -0.0197655059726074 2201 66-67 0.058936134895894554 2201 68-69 -0.18376885442537372 2201 70-71 -0.01256284796196283 2201 72-73 0.24524273225284432 2201 74-75 0.23682065778255534 2201 76-77 0.046167580664754126 2201 78-79 0.21849693526117164 2201 80-81 0.2185866771096805 2201 82-83 0.19563000675022835 2201 84-85 0.16227431302482387 2201 86-87 0.16380788015780112 2201 88-89 0.14984618356581692 2201 90-91 0.1250394974750506 2201 92-93 0.15131564119991125 2201 94-95 0.12743728188013392 2201 96-97 0.14644419944391274 2201 98-99 0.1510193528501631 2201 100-101 -0.0946420948972495 2304 1 2.3068010640547705 2304 2 1.3433548652638052 2304 3 2.1873156425407423 2304 4 2.4985678294012317 2304 5 1.845000597206237 2304 6 0.9020859363748315 2304 7 0.48156051694579816 2304 8 0.3053805217984973 2304 9 0.33539079881987277 2304 10-11 0.24411371575722995 2304 12-13 0.18480895075870762 2304 14-15 0.2019692330300913 2304 16-17 0.24224783574118192 2304 18-19 0.26908736998307603 2304 20-21 0.27619602086566175 2304 22-23 0.25072986572325107 2304 24-25 0.284623572192892 2304 26-27 0.28995438844494004 2304 28-29 0.4076106261877115 2304 30-31 -1.1591994452676744 2304 32-33 -0.6894734640931617 2304 34-35 0.11661459648665584 2304 36-37 0.5593196861953729 2304 38-39 0.5564188889417707 2304 40-41 0.36124395955004474 2304 42-43 0.24248368944100918 2304 44-45 0.2774522793733709 2304 46-47 0.2295719054156109 2304 48-49 0.24293034902207467 2304 50-51 0.2561469942944754 2304 52-53 0.29455500935423373 2304 54-55 0.24036558489940774 2304 56-57 0.27349786004705834 2304 58-59 0.24650180869117122 2304 60-61 0.1653344996150139 2304 62-63 0.21439143749756795 2304 64-65 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0.6361347427316986 2203 3 0.8070464123045404 2203 4 0.33211978017803645 2203 5 0.6698513732596609 2203 6 0.3202693205716969 2203 7 0.15029875421892314 2203 8 0.08111641739974118 2203 9 0.034838591117818396 2203 10-11 0.10046398375644117 2203 12-13 0.09029584619082698 2203 14-15 0.03430685390343058 2203 16-17 0.07613608025894081 2203 18-19 0.07838083251527195 2203 20-21 0.07740219238278456 2203 22-23 0.10187793488626085 2203 24-25 0.06804931348818855 2203 26-27 0.02033787821852684 2203 28-29 0.03540216785281558 2203 30-31 0.10377934748929363 2203 32-33 0.16677732999122696 2203 34-35 0.1420396933043051 2203 36-37 0.3622221439993325 2203 38-39 0.26437423955757566 2203 40-41 0.1088740979424827 2203 42-43 0.016480545673069003 2203 44-45 0.025663964280333573 2203 46-47 0.10638483294825107 2203 48-49 0.10642272788928153 2203 50-51 0.0021910485348470843 2203 52-53 0.07895000210394443 2203 54-55 0.01599641367495508 2203 56-57 0.07923229916714547 2203 58-59 0.11525620148685789 2203 60-61 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2213 90-91 -0.17094556264030203 2213 92-93 -0.18329064925739047 2213 94-95 -0.10603891306061897 2213 96-97 -0.07263761204578856 2213 98-99 -0.060688305605332005 2213 100-101 0.12802767492830824 2212 1 -1.7765057810910925 2212 2 -0.5213496896913767 2212 3 -2.282924639278651 2212 4 -2.7429794898332673 2212 5 -0.497980446887361 2212 6 -0.3387101926766931 2212 7 -0.18083550693211947 2212 8 -0.16274922637369116 2212 9 -0.11610267560489973 2212 10-11 -0.04333515792006182 2212 12-13 -0.14092853950529616 2212 14-15 -0.17690828730220431 2212 16-17 -0.18342318660943846 2212 18-19 -0.1981480109329894 2212 20-21 -0.2553825939614569 2212 22-23 -0.23668041898913827 2212 24-25 -0.13343335416392677 2212 26-27 -0.13805396722455043 2212 28-29 -0.1295287558657563 2212 30-31 -0.16663658402262627 2212 32-33 -0.19572616000913712 2212 34-35 -0.40605883547518573 2212 36-37 -0.2692622270677063 2212 38-39 -0.3184883101033975 2212 40-41 -0.26816685447725064 2212 42-43 -0.20874397629248875 2212 44-45 -0.18040777795638263 2212 46-47 -0.18633744527020468 2212 48-49 -0.1809569252425831 2212 50-51 -0.15571734785988411 2212 52-53 -0.14805472383826412 2212 54-55 -0.1772800692901484 2212 56-57 -0.29376856401518836 2212 58-59 -0.10256905786143022 2212 60-61 -0.03698376073823795 2212 62-63 -0.10714196000242282 2212 64-65 0.10269827044552926 2212 66-67 0.06260075845907664 2212 68-69 0.24999106229980583 2212 70-71 0.20156677732552097 2212 72-73 -0.5175780626015225 2212 74-75 -0.5389462953322521 2212 76-77 -0.3073648338815751 2212 78-79 -0.35431939464154283 2212 80-81 -0.3714118211755775 2212 82-83 -0.36705616610245784 2212 84-85 -0.3658184528013706 2212 86-87 -0.33934281720983606 2212 88-89 -0.347555998208243 2212 90-91 -0.31944620832656057 2212 92-93 -0.3167786657230742 2212 94-95 -0.24485981573966242 2212 96-97 -0.2270899808103799 2212 98-99 -0.24694892346046515 2212 100-101 -0.07424224271438717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27260.0 3 858.0 4 1337.0 5 2065.0 6 3096.0 7 5888.0 8 9887.0 9 14179.0 10 18494.0 11 23855.0 12 29357.0 13 35955.0 14 39188.0 15 42510.0 16 46568.0 17 50788.0 18 59256.0 19 72574.0 20 90946.0 21 113522.0 22 142576.0 23 184623.0 24 253534.0 25 383311.0 26 598305.0 27 821237.0 28 313552.0 29 305846.0 30 372012.0 31 462902.0 32 579240.0 33 744461.0 34 997082.0 35 1393273.0 36 2097815.0 37 3038306.0 38 2310749.0 39 313332.0 40 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.903794767537466 23.710064227489173 13.390109292920114 35.996031712053245 2 16.885547549090383 36.46284243889055 22.273779857833677 24.377830154185393 3 12.845712944159693 15.467903016139578 39.49368493802886 32.19269910167186 4 16.386084911778244 18.595352532298907 14.504958401060591 50.51360415486226 5 31.780190150950755 21.868315591577957 16.784077670388353 29.567416587082935 6 29.567517337818998 26.853203819951432 19.181975943240978 24.39730289898859 7 10.225300140812587 43.69763981102488 27.021316888323057 19.055743159839476 8 9.921217982207594 45.24908826397248 22.488484510378175 22.341209243441753 9 22.330272269494117 37.34250383734543 23.390097733167757 16.937126159992694 10-11 15.9627125 41.056659374999995 25.750849999999996 17.229778125 12-13 8.910144863768107 44.5739711967464 22.64352783044098 23.872356109044514 14-15 9.8005621314081 40.800355225099906 22.7442876468309 26.654794996661092 16-17 16.144226513521236 36.700412815663704 22.766867759393854 24.38849291142121 18-19 13.009615855469322 30.278986902515285 29.659817397902454 27.051579844112933 20-21 21.976900170897 30.441709379978615 29.6337494242574 17.947641024866986 22-23 8.737329638265917 46.651740729318206 23.145364419258296 21.46556521315758 24-25 8.897697298272973 33.57675643256732 29.161631246223436 28.363915022936265 26-27 9.38877715618715 33.265415586796266 25.988736493664277 31.357070763352308 28-29 15.617297914800057 39.975169264037646 22.174092840477382 22.233439980684913 30-31 8.827256277718567 30.428923190116226 32.33066858434759 28.41315194781761 32-33 8.90266476441084 40.055438294779776 24.08607063859133 26.95582630221805 34-35 9.178716424602738 40.136436316708206 29.91998474471301 20.76486251397604 36-37 9.782847453527523 39.916946498663826 23.66469785566216 26.635508192146496 38-39 15.489679904071755 33.16294867419437 24.372886526523285 26.974484895210594 40-41 9.10204921944185 39.45839151339537 30.626834660248125 20.812724606914657 42-43 9.474948398456732 32.78116051609856 30.851666259663002 26.892224825781703 44-45 15.765258250001812 33.1903330102328 24.385982928590945 26.658425811174446 46-47 9.680040491669377 39.104045244658785 30.25450589365374 20.961408370018102 48-49 16.133416657798204 32.581459743985114 30.211891363775518 21.073232234441164 50-51 9.242443114431264 32.628623608267034 24.31055858105163 33.818374696250075 52-53 15.507558685198061 32.68387473511844 31.305329656588114 20.503236923095386 54-55 9.043781687557763 32.6616218773088 31.10198611743822 27.192610317695227 56-57 9.429872615437922 32.56818887220406 30.617788938404484 27.384149573953533 58-59 15.706325894584841 32.77933244765692 31.152561428175712 20.36178022958253 60-61 9.446649105765285 33.411895096093495 30.58099037633771 26.56046542180351 62-63 15.582592868222052 34.20055049641116 30.147047380329468 20.069809255037317 64-65 9.025896679285982 47.09283146576973 23.6191087745281 20.262163080416194 66-67 8.999726272276863 47.052086176199964 23.77222615828875 20.17596139323442 68-69 9.079988518626218 46.963765703098986 23.811656719424008 20.14458905885079 70-71 9.235179211238794 45.980295782639224 24.096828057387498 20.687696948734484 72-73 9.520197163023713 44.36486471917191 24.626784164895437 21.488153952908938 74-75 9.796172922732074 43.06652559899489 24.975346699345234 22.1619547789278 76-77 10.068768053371885 41.72855791470136 25.343744950993578 22.858929080933173 78-79 10.280812856888032 40.92272036978541 25.549129906024877 23.247336867301684 80-81 10.555202238357763 40.242430215929815 25.689129734168763 23.513237811543657 82-83 10.760073650708648 39.67636764340202 25.559360969255778 24.004197736633557 84-85 11.026653576629032 38.81604597113217 25.80676481944886 24.350535632789942 86-87 11.284395610659345 38.498402670726925 25.80954980061392 24.407651917999814 88-89 11.501654754163823 38.17047419556347 25.631034307214414 24.69683674305829 90-91 11.732044624312369 37.753533024731176 25.643782631478416 24.870639719478035 92-93 11.980975040576743 37.48604134430846 25.56520407059306 24.96777954452173 94-95 12.129393008786018 37.47780308060616 25.35507571015142 25.0377282004564 96-97 12.38194447460969 37.0706763917262 25.301132039905287 25.246247093758818 98-99 12.591975577450393 36.9200593688706 25.168854249235196 25.319110804443824 100-101 13.022102364824567 36.49796925794625 24.849528337279025 25.630400039950153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 15.5 2 24.0 3 36.5 4 62.0 5 102.5 6 153.0 7 247.5 8 393.0 9 659.0 10 1229.0 11 2363.5 12 4336.5 13 7426.0 14 12230.0 15 18561.5 16 26592.5 17 36915.5 18 50074.0 19 67392.0 20 90106.5 21 119895.5 22 158986.5 23 208485.5 24 266699.5 25 331106.0 26 399551.0 27 469133.0 28 533278.0 29 588824.5 30 632656.5 31 663036.5 32 681599.0 33 686395.5 34 678475.5 35 663497.0 36 643431.5 37 622017.5 38 606042.5 39 594912.0 40 588031.0 41 584466.0 42 576902.5 43 558203.0 44 529018.0 45 491428.5 46 444263.5 47 389858.0 48 332918.5 49 277579.0 50 227524.0 51 184043.0 52 146878.0 53 117606.5 54 95891.0 55 78744.0 56 65790.0 57 56085.5 58 48660.5 59 43017.5 60 38264.0 61 34432.0 62 31344.0 63 28095.5 64 24889.0 65 22117.5 66 19173.5 67 16285.0 68 13734.5 69 11406.0 70 9429.5 71 7841.0 72 6445.5 73 5179.5 74 4171.5 75 3411.0 76 2762.5 77 2262.5 78 1993.5 79 1728.5 80 1439.5 81 1278.0 82 1172.0 83 1016.0 84 916.5 85 858.5 86 791.5 87 649.5 88 560.5 89 534.0 90 463.5 91 378.0 92 318.0 93 243.5 94 185.5 95 151.0 96 96.0 97 61.0 98 38.5 99 18.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.562500000000001E-4 2 0.0026875 3 0.00939375 4 0.008718749999999999 5 5.0E-4 6 3.7500000000000003E-5 7 6.25E-5 8 1.8125E-4 9 4.375E-5 10-11 0.0 12-13 1.2499999999999999E-5 14-15 2.8125E-5 16-17 9.375000000000001E-6 18-19 1.21875E-4 20-21 7.1875E-5 22-23 6.96875E-4 24-25 7.500000000000001E-5 26-27 5.625E-5 28-29 4.46875E-4 30-31 7.4375E-4 32-33 3.0625E-4 34-35 0.00188125 36-37 4.53125E-4 38-39 7.78125E-4 40-41 6.4375E-4 42-43 0.00601875 44-45 0.005325 46-47 0.010296875 48-49 0.014359375 50-51 0.019265625 52-53 0.001003125 54-55 0.004496875 56-57 6.375E-4 58-59 0.0011 60-61 3.8750000000000004E-4 62-63 0.00161875 64-65 0.0011749999999999998 66-67 0.0032781250000000002 68-69 0.0010781249999999999 70-71 0.0017375 72-73 2.65625E-4 74-75 7.125E-4 76-77 0.0035312499999999997 78-79 0.0014625 80-81 3.46875E-4 82-83 0.002659375 84-85 2.875E-4 86-87 7.1875E-5 88-89 0.0030843750000000003 90-91 7.0E-4 92-93 4.4375E-4 94-95 1.9999999999999998E-4 96-97 0.001040625 98-99 2.03125E-4 100-101 3.78125E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.85442314324729 #Duplication Level Percentage of deduplicated Percentage of total 1 89.96579215010017 64.64440097570733 2 7.0708938990408905 10.161500044453797 3 1.3377797992775808 2.8837618730933916 4 0.6449148823546484 1.8535994739235382 5 0.2809435348461939 1.0093517816099027 6 0.18327700828565222 0.7901558223471414 7 0.11567248883733175 0.5818105971265128 8 0.08596076556226208 0.49413289779426073 9 0.049255952479823464 0.31853322466280237 >10 0.2482981141677693 2.9649236962170615 >50 0.009663127545066949 0.48087895698646416 >100 0.0064834262000642365 0.9144051787202087 >500 6.124336048601436E-4 0.2912435020778088 >1k 3.456737238869562E-4 0.5795450145301869 >5k 3.616944582882615E-5 0.16837286796425097 >10k+ 7.057452794900041E-5 11.863384092785324 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 1522430 9.5151875 TruSeq Adapter, Index 1 (100% over 50bp) GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 98641 0.61650625 TruSeq Adapter, Index 1 (98% over 50bp) CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT 97647 0.61029375 TruSeq Adapter, Index 1 (100% over 50bp) GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 68249 0.42655625 TruSeq Adapter, Index 1 (98% over 50bp) GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 41983 0.26239375 TruSeq Adapter, Index 1 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC 18921 0.11825625000000001 TruSeq Adapter, Index 1 (98% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.375E-4 0.0 1.875E-5 2 0.00110625 0.0 1.875E-5 3 0.00124375 0.0 1.875E-5 4 0.00135 0.0 1.875E-5 5 0.0015625 0.0 1.875E-5 6 0.001975 0.0 1.875E-5 7 0.00241875 0.0 1.875E-5 8 0.00293125 0.0 1.875E-5 9 0.0031625 0.0 3.125E-5 10-11 0.004884375 0.0 3.4374999999999995E-5 12-13 0.007237500000000001 0.0 3.75E-5 14-15 0.010909374999999999 0.0 4.375E-5 16-17 0.01375 0.0 4.375E-5 18-19 0.015178125 0.0 4.375E-5 20-21 0.017153124999999998 6.25E-6 4.375E-5 22-23 0.02304375 6.25E-6 4.6875E-5 24-25 0.034446875 6.25E-6 5.625E-5 26-27 0.040059375 1.25E-5 5.625E-5 28-29 0.042159375 1.875E-5 5.625E-5 30-31 0.044046875 2.1875E-5 5.625E-5 32-33 0.046415625 2.5E-5 5.625E-5 34-35 0.048681249999999995 2.5E-5 5.625E-5 36-37 0.051206249999999995 2.5E-5 5.625E-5 38-39 0.054025 2.5E-5 5.625E-5 40-41 0.056953125 2.5E-5 5.625E-5 42-43 0.060709374999999996 2.5E-5 6.875E-5 44-45 0.06533749999999999 2.5E-5 6.875E-5 46-47 0.070903125 3.125E-5 6.875E-5 48-49 0.0783375 4.375E-5 6.875E-5 50-51 0.0869 4.375E-5 6.875E-5 52-53 0.097646875 4.375E-5 7.812499999999999E-5 54-55 0.11125312500000001 4.375E-5 8.125E-5 56-57 0.12836875 4.375E-5 8.125E-5 58-59 0.1509625 4.375E-5 8.125E-5 60-61 0.18046562500000002 4.375E-5 8.125E-5 62-63 0.218990625 5.0E-5 8.75E-5 64-65 0.26915 5.0E-5 8.75E-5 66-67 0.333815625 5.0E-5 8.75E-5 68-69 0.4170125 5.0E-5 8.75E-5 70-71 0.52228125 5.0E-5 8.75E-5 72-73 0.65520625 5.3125000000000004E-5 9.0625E-5 74-75 0.821196875 5.625E-5 9.375E-5 76-77 1.0233937499999999 5.625E-5 9.375E-5 78-79 1.2664312500000001 5.625E-5 9.375E-5 80-81 1.555215625 5.625E-5 1.0E-4 82-83 1.8982687500000002 5.625E-5 1.0625E-4 84-85 2.30066875 5.625E-5 1.0937499999999999E-4 86-87 2.767984375 5.9375E-5 1.125E-4 88-89 3.303575 6.25E-5 1.15625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGGA 13595 0.0 81.698166 1 CCGGAAG 14445 0.0 80.174965 3 ACCGGAA 15035 0.0 76.842545 2 GAGCACA 276855 0.0 72.769 9 AGAGCAC 280020 0.0 71.96709 8 AAGAGCA 285105 0.0 70.75515 7 GAAGAGC 285330 0.0 70.73431 6 GGAAGAG 291370 0.0 68.87254 5 CGGAAGA 283915 0.0 67.79651 4 TCGGAAG 258260 0.0 67.18242 3 ATCGGAA 264820 0.0 65.49956 2 GATCGGA 266665 0.0 63.848255 1 ACGGAAG 8180 0.0 62.079323 3 AATCATA 159335 0.0 61.50571 5 GAACGGA 8115 0.0 60.0043 1 ATCATAA 167340 0.0 59.510925 6 CATAAAC 167090 0.0 59.102516 8 GATTGGA 13355 0.0 59.084396 1 TCATAAA 167875 0.0 58.905373 7 TAAATCA 152125 0.0 58.20602 3 >>END_MODULE