##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_ATCACG_L001_R1_003.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1409414
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.470590614255286	28.0	16.0	31.0	10.0	31.0
2	27.40011380616341	30.0	25.0	31.0	16.0	33.0
3	24.05945236814733	27.0	16.0	31.0	10.0	31.0
4	29.37608254210615	33.0	26.0	35.0	19.0	37.0
5	31.574017286616993	35.0	32.0	35.0	19.0	37.0
6	33.44716314723708	35.0	32.0	36.0	28.0	37.0
7	34.28438485782034	35.0	35.0	37.0	31.0	37.0
8	34.515944215113514	35.0	35.0	37.0	31.0	37.0
9	36.144298268642146	37.0	35.0	39.0	32.0	39.0
10-11	36.41600764573078	38.0	35.0	39.0	32.0	39.0
12-13	36.4183838105766	38.0	35.0	39.0	32.0	39.0
14-15	37.582180253637326	39.0	36.0	40.0	32.0	41.0
16-17	37.53936068465334	39.0	36.0	40.0	32.0	41.0
18-19	37.63577132056301	39.5	36.5	40.0	32.0	41.0
20-21	37.43796003161597	39.0	36.0	40.0	32.0	41.0
22-23	37.50061550403217	39.0	36.5	40.0	32.0	41.0
24-25	37.52572771378743	39.0	36.5	40.0	32.0	41.0
26-27	37.295084694773855	39.0	36.0	40.0	31.5	41.0
28-29	37.06819323491891	39.0	36.0	40.0	30.5	41.0
30-31	37.033672859784275	39.0	36.0	40.0	31.0	41.0
32-33	36.94962445385103	39.0	36.0	40.0	30.5	41.0
34-35	36.21503014728107	38.5	35.0	40.0	29.5	41.0
36-37	35.92604834349595	38.0	35.0	40.0	28.0	41.0
38-39	35.6977889392329	38.0	34.5	40.0	26.5	41.0
40-41	36.19829517799596	38.5	35.0	40.0	30.0	41.0
42-43	36.36937372553416	39.0	35.0	40.0	30.0	41.0
44-45	36.26121210659181	39.0	35.0	40.0	29.5	41.0
46-47	36.105572244918804	39.0	35.0	40.0	29.5	41.0
48-49	36.04692907832617	38.5	35.0	40.0	28.5	41.0
50-51	35.96273486711499	38.5	35.0	40.0	28.5	41.0
52-53	35.58523081223828	38.0	34.0	40.0	27.5	41.0
54-55	35.5585158086978	38.0	34.0	40.0	28.0	41.0
56-57	35.38875305623472	38.0	34.0	40.0	28.0	41.0
58-59	35.094483239133424	37.5	34.0	40.0	27.5	41.0
60-61	34.88221736125794	37.0	34.0	39.5	27.0	41.0
62-63	34.34323307417125	36.5	33.0	39.0	26.0	40.5
64-65	34.21916910148473	36.0	33.0	39.0	26.0	40.0
66-67	33.84674730065119	36.0	33.0	39.0	26.0	40.0
68-69	33.24414331062413	35.5	33.0	38.0	26.0	40.0
70-71	31.361787239235596	35.0	31.0	37.0	10.5	40.0
72-73	29.474103776463124	35.0	29.5	37.0	2.0	39.5
74-75	28.936746761419993	34.5	29.0	36.0	2.0	39.0
76-77	27.327038045599092	32.5	26.0	34.5	2.0	36.5
78-79	28.408695741634467	34.0	29.0	35.5	2.0	37.0
80-81	28.373230292873494	34.0	29.0	35.0	2.0	37.0
82-83	28.20479717102285	34.0	29.0	35.0	2.0	37.0
84-85	28.00299131412062	34.0	29.0	35.0	2.0	36.0
86-87	27.80132984346686	34.0	29.0	35.0	2.0	36.0
88-89	27.62028190439431	34.0	29.0	35.0	2.0	36.0
90-91	27.44724545094628	34.0	29.0	35.0	2.0	36.0
92-93	27.304838393828923	34.0	28.5	35.0	2.0	35.0
94-95	27.12069271342558	34.0	27.0	35.0	2.0	35.0
96-97	26.945258454932333	34.0	27.0	35.0	2.0	35.0
98-99	26.708548730181477	34.0	27.0	35.0	2.0	35.0
100-101	25.87480364179723	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2314	1	0.23441582318798382
2314	2	1.1598320732963394
2314	3	-1.6256611462905646
2314	4	-2.1731599651636913
2314	5	0.29696813839485614
2314	6	0.13304627066755614
2314	7	0.1333883239680631
2314	8	0.24024545195114655
2314	9	0.386535991456725
2314	10-11	0.3207320894494714
2314	12-13	0.2902790608550063
2314	14-15	0.3276334394887499
2314	16-17	0.3168771024506327
2314	18-19	0.2616993679275197
2314	20-21	0.45570977830711
2314	22-23	0.3572198514034568
2314	24-25	0.3144751349279744
2314	26-27	0.29316358422979505
2314	28-29	0.42682995773388654
2314	30-31	0.42670442987387247
2314	32-33	0.37814105868898196
2314	34-35	0.4155879567963794
2314	36-37	0.41536471177956
2314	38-39	0.41468630910109283
2314	40-41	0.4372079330701979
2314	42-43	0.43196807422968675
2314	44-45	0.4277468732506122
2314	46-47	0.48152413517495773
2314	48-49	0.4505256157091253
2314	50-51	0.4572668414200578
2314	52-53	0.5234609559192194
2314	54-55	0.41806000404036325
2314	56-57	0.42583701645516925
2314	58-59	0.4419343778072573
2314	60-61	0.3542548090579274
2314	62-63	0.411782568865398
2314	64-65	0.3326771730081859
2314	66-67	0.2861053393009172
2314	68-69	0.1613694546065787
2314	70-71	-0.21777657000987105
2314	72-73	-0.08583613212100616
2314	74-75	0.02450711810289974
2314	76-77	-0.011433769516152381
2314	78-79	0.09190841488775092
2314	80-81	0.08081142023532095
2314	82-83	0.06256970979791276
2314	84-85	0.047346533843366956
2314	86-87	0.11900423233579716
2314	88-89	0.11718295344401852
2314	90-91	0.1278422550344409
2314	92-93	0.12138376110624094
2314	94-95	0.11777243260277359
2314	96-97	0.09742927127457435
2314	98-99	0.104283030143975
2314	100-101	0.0610649606541287
2315	1	2.4244326690290663
2315	2	2.139857919388543
2315	3	2.4722793949862094
2315	4	1.0637900235301885
2315	5	1.720997661622519
2315	6	0.861062318451232
2315	7	0.5160077058951344
2315	8	0.34823903765332886
2315	9	0.40245954561035546
2315	10-11	0.30077699364326804
2315	12-13	0.3016432586995492
2315	14-15	0.22862501280478398
2315	16-17	0.3233266699048869
2315	18-19	0.32550960653185257
2315	20-21	0.4243203591757876
2315	22-23	0.3842024827246604
2315	24-25	0.31933325825345094
2315	26-27	0.33020249537020163
2315	28-29	0.40965816080951356
2315	30-31	0.3995817808855051
2315	32-33	0.30934968075041525
2315	34-35	0.336922376825207
2315	36-37	0.3637510437595495
2315	38-39	0.28560526088303817
2315	40-41	0.38275826573156024
2315	42-43	0.27658070624202935
2315	44-45	0.41374218987887446
2315	46-47	0.2737455908339257
2315	48-49	0.33288319475398964
2315	50-51	0.3089248602567167
2315	52-53	0.28935415449453217
2315	54-55	0.2706411719343862
2315	56-57	0.2583986178011415
2315	58-59	0.3382832013388608
2315	60-61	0.2799179402200096
2315	62-63	0.31572786951205956
2315	64-65	0.25569856672809266
2315	66-67	0.22028005903396775
2315	68-69	0.1591470722040853
2315	70-71	-0.306407507553935
2315	72-73	-0.16986180916627802
2315	74-75	-0.06625193187680267
2315	76-77	-0.17175625264114913
2315	78-79	-0.12902311604769778
2315	80-81	-0.09167557139346627
2315	82-83	-0.08143193585586772
2315	84-85	-0.032651459060407007
2315	86-87	-0.10030699706229029
2315	88-89	-0.03402867225332784
2315	90-91	0.0025812103576576817
2315	92-93	-0.06222639056872836
2315	94-95	-0.04097104777869731
2315	96-97	-0.07597404820298692
2315	98-99	-0.09888983991348255
2315	100-101	-0.17072123200424016
2312	1	-1.9218774073109834
2312	2	-0.4098758556383366
2312	3	-2.3020158753750692
2312	4	-2.3382099216102077
2312	5	-0.059133429916105484
2312	6	-0.14984613769306776
2312	7	-0.06411568603236617
2312	8	-2.0817180747911834E-4
2312	9	0.008289418047517927
2312	10-11	-0.029247792864936173
2312	12-13	0.06339880610188686
2312	14-15	0.07624346519806124
2312	16-17	0.042627939267092074
2312	18-19	-0.054711816297114524
2312	20-21	0.052352094672393434
2312	22-23	-0.11493652559640566
2312	24-25	0.003424387462331424
2312	26-27	-0.055840429517594714
2312	28-29	-0.0045665479550578425
2312	30-31	0.009468941431798328
2312	32-33	-0.049055151046786705
2312	34-35	-0.06863121168358788
2312	36-37	0.050258876964150545
2312	38-39	0.02086860805884072
2312	40-41	-0.10751455051239844
2312	42-43	-0.07274818724092569
2312	44-45	-0.09904956990532554
2312	46-47	-0.05043483076755706
2312	48-49	-0.11460692536908823
2312	50-51	-0.07829025332320327
2312	52-53	-0.046193503094258404
2312	54-55	-0.1231025491117208
2312	56-57	-0.027434174235018816
2312	58-59	-0.10690375843715572
2312	60-61	-0.20411599455226082
2312	62-63	-0.07120014003961472
2312	64-65	-0.0946990324556154
2312	66-67	-0.016602768448009897
2312	68-69	-0.06002174318420117
2312	70-71	-0.2122517317884629
2312	72-73	-0.18058808886562971
2312	74-75	-0.19048893416544033
2312	76-77	-0.0717878215063088
2312	78-79	-0.1612968488786386
2312	80-81	-0.19906978199939118
2312	82-83	-0.2009762674468476
2312	84-85	-0.19826677541184168
2312	86-87	-0.17383885092947793
2312	88-89	-0.16072737619320066
2312	90-91	-0.14379250027788615
2312	92-93	-0.15087456665394328
2312	94-95	-0.09775210174704085
2312	96-97	-0.12764978623746615
2312	98-99	-0.12410039052663535
2312	100-101	-0.10464160534173317
2313	1	-0.004197436341698335
2313	2	0.699214589207223
2313	3	-0.5126096590398532
2313	4	0.13313249137598504
2313	5	1.263925200085211
2313	6	0.4465156440518001
2313	7	0.25664418640998576
2313	8	-0.002796147012936956
2313	9	-0.019810810571470938
2313	10-11	0.05734584211453608
2313	12-13	-0.15906104545562982
2313	14-15	-0.20620388700812953
2313	16-17	-0.0832872795940034
2313	18-19	-0.10766076472680197
2313	20-21	-0.10815323499342355
2313	22-23	-0.09699196372231711
2313	24-25	-0.1502160457533961
2313	26-27	-0.04363560793267851
2313	28-29	-0.07028295511572935
2313	30-31	-0.022712643130084587
2313	32-33	-0.04778011969608542
2313	34-35	-0.13634299539362615
2313	36-37	-0.07896095545731896
2313	38-39	-0.13544064499516395
2313	40-41	-0.04468987015589221
2313	42-43	-0.02924744237778043
2313	44-45	-0.04679875038133474
2313	46-47	-0.07214188857288661
2313	48-49	-0.056018969247546124
2313	50-51	-0.09138846616610152
2313	52-53	-0.005334311666260305
2313	54-55	0.0520023308445019
2313	56-57	0.009314957266063573
2313	58-59	0.013299998274725056
2313	60-61	0.008045082846940943
2313	62-63	0.04854855897161059
2313	64-65	0.05661253783777198
2313	66-67	-0.03927434775565075
2313	68-69	-0.016706337649857517
2313	70-71	0.2861492343299048
2313	72-73	0.019361214354066902
2313	74-75	-0.10027021274152759
2313	76-77	-0.12947172026284548
2313	78-79	-0.06649466519004221
2313	80-81	-0.008299507949640628
2313	82-83	-0.02998002412672207
2313	84-85	-0.05058572274153761
2313	86-87	-0.0805836023912363
2313	88-89	-0.09218256819002235
2313	90-91	-0.096217392078632
2313	92-93	-0.08902751083978089
2313	94-95	-0.05955869018344373
2313	96-97	-0.07492996476409175
2313	98-99	-0.09331775507234852
2313	100-101	0.02761428071647032
2316	1	-0.7327736485643719
2316	2	-3.5890287262537726
2316	3	1.9680072857192847
2316	4	3.314447371867729
2316	5	-3.2227575701864737
2316	6	-1.2907780954775063
2316	7	-0.8419245302408243
2316	8	-0.5854801707840593
2316	9	-0.7774741445431346
2316	10-11	-0.6496071323423678
2316	12-13	-0.49626008020079837
2316	14-15	-0.4262980304834372
2316	16-17	-0.5995444320286012
2316	18-19	-0.42483639343544155
2316	20-21	-0.8242289971618604
2316	22-23	-0.5294938448093802
2316	24-25	-0.48701673489032515
2316	26-27	-0.5238900421497092
2316	28-29	-0.7616386154726342
2316	30-31	-0.81304250906107
2316	32-33	-0.5906554686965393
2316	34-35	-0.5475361265443937
2316	36-37	-0.7504136770459553
2316	38-39	-0.5857195330477936
2316	40-41	-0.6677617781334675
2316	42-43	-0.6065531508530313
2316	44-45	-0.6956407428428335
2316	46-47	-0.6326930066684184
2316	48-49	-0.6127829158464948
2316	50-51	-0.5965129821874626
2316	52-53	-0.7612872956532399
2316	54-55	-0.6176009577075234
2316	56-57	-0.6661164172873413
2316	58-59	-0.6866138189836661
2316	60-61	-0.4381018375726171
2316	62-63	-0.7048588573094605
2316	64-65	-0.5502892451184209
2316	66-67	-0.4505082821312314
2316	68-69	-0.24378844597660532
2316	70-71	0.45028657502234637
2316	72-73	0.41692481579886476
2316	74-75	0.3325039606808602
2316	76-77	0.3844495639264487
2316	78-79	0.2649062152286099
2316	80-81	0.21823344110719844
2316	82-83	0.24981851763153173
2316	84-85	0.23415742337041934
2316	86-87	0.23572521804720026
2316	88-89	0.16975566319254654
2316	90-91	0.1095864269644089
2316	92-93	0.18074470695623646
2316	94-95	0.08050940710640475
2316	96-97	0.1811245279299456
2316	98-99	0.2120249553684843
2316	100-101	0.18668359597538853
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1910.0
3	50.0
4	94.0
5	162.0
6	276.0
7	555.0
8	855.0
9	1314.0
10	1699.0
11	2149.0
12	2796.0
13	3420.0
14	3453.0
15	3746.0
16	3820.0
17	4052.0
18	4906.0
19	6216.0
20	8302.0
21	10736.0
22	13882.0
23	18226.0
24	24650.0
25	36157.0
26	53173.0
27	65417.0
28	42798.0
29	34446.0
30	42469.0
31	53013.0
32	65401.0
33	81865.0
34	106860.0
35	143639.0
36	195959.0
37	228824.0
38	129504.0
39	12613.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.7572549631753	27.004143666009167	15.033206090621409	34.205395280194125
2	16.334162996094815	36.95771557987467	21.90678639542276	24.801335028607756
3	12.701583147904275	14.379030243858134	41.756661255165525	31.16272535307207
4	17.082461672963436	19.71497060718655	12.288850032784836	50.91371768706517
5	29.876948597676616	22.3372032714375	19.47416332070631	28.31168481017957
6	29.711568070134113	27.394292947281635	18.84066711413396	24.05347186845029
7	10.038427318020112	43.96962141712797	27.370949912516835	18.621001352335085
8	9.646917087527157	45.55070405147104	22.11571617707785	22.68666268392396
9	22.62223874603204	37.80301600523338	23.093427481208504	16.481317767526075
10-11	16.02045815511204	41.58414120483449	25.81013308017839	16.585267559875085
12-13	8.697550897039479	45.26473413773384	22.328428694478696	23.709286270747985
14-15	9.547549548961483	41.208402924903545	22.37497286106141	26.869074665073573
16-17	16.1814471054804	37.21207438413212	22.405135185664108	24.201343324723368
18-19	13.157063846544084	30.021179300338087	29.535935631955557	27.28582122116227
20-21	22.78840709685018	30.186127000299418	29.52947111352661	17.49599478932379
22-23	8.520314116363254	47.36071161489811	22.820902871689935	21.2980713970487
24-25	8.67506235749835	33.706325361577704	29.137510035202585	28.481102245721356
26-27	9.143764713561806	33.38100089824565	26.000061018267168	31.475173369925376
28-29	15.616435926316816	40.373701808377604	21.875072060166914	22.134790205138664
30-31	8.592578192071315	30.231535943306937	32.61983349108211	28.556052373539643
32-33	8.701542749234692	40.46485755103503	23.925544176793025	26.908055522937254
34-35	9.04783697680524	40.41479526313175	29.944301354505914	20.593066405557092
36-37	9.451077067600488	40.43885879542828	23.570399008660033	26.539665128311192
38-39	15.497215852329488	33.37880159613114	24.299315741077105	26.824666810462265
40-41	8.912008048740859	39.805223876890594	30.68305741468257	20.599710659685982
42-43	9.300423085137103	32.87008951979163	30.90948049856358	26.920006896507687
44-45	15.841010166593048	33.26961251283907	24.19283708438219	26.69654023618569
46-47	9.441777750022709	39.49016855527296	30.3107116904351	20.757342004269233
48-49	16.17816620037507	32.70567858731739	30.283741335526308	20.832413876781235
50-51	9.09408669497498	32.78321855999501	24.07330258358889	34.049392161441126
52-53	15.599178098131564	32.79508363050175	31.288996703594545	20.316741567772137
54-55	8.87128502124112	32.7426853387493	31.241811747802718	27.144217892206857
56-57	9.217767100369374	32.665845521613946	30.739761347623904	27.376626030392774
58-59	15.80880422643737	32.873485008663174	31.1133208553342	20.20438990956525
60-61	9.256384239319393	33.4521723088276	30.732936927476178	26.558506524376828
62-63	15.69093372432894	34.191196425980394	30.215266353931675	19.902603495758992
64-65	8.858180419726299	47.63794863862484	23.477929849781496	20.02594109186737
66-67	8.829449686181633	47.59346792354837	23.591081115981535	19.986001274288462
68-69	8.876561464551934	47.548733019538616	23.680089739423618	19.89461577648583
70-71	8.94343322827785	47.14097490162578	23.764912225932196	20.15067964416417
72-73	9.217660673159198	45.87197232324924	24.196261708766905	20.71410529482466
74-75	9.61950458080938	43.69803020762197	24.918254946653303	21.764210264915345
76-77	10.109059018734907	41.74050261303862	25.488186425964145	22.66225194226233
78-79	10.360186479524861	40.704797519283034	25.872260106296167	23.06275589489594
80-81	10.680036682991899	39.74194189257985	26.10115494661631	23.476866477811935
82-83	11.164673595847459	38.56889177456923	25.977793270569787	24.288641359013525
84-85	11.355180238028003	38.101579805507825	26.2028048536484	24.34043510281578
86-87	11.687055755086865	37.6027909471596	26.184463897761766	24.52568939999177
88-89	11.85776500020576	37.354035081246536	26.03883599850718	24.749363920040526
90-91	12.091690503752146	37.00964280025415	25.94353528663216	24.955131409361545
92-93	12.28536071179458	36.854769411111896	25.919208562084407	24.940661315009123
94-95	12.47323355664127	36.840984976735015	25.674961366922705	25.010820099701007
96-97	12.58379100947967	36.83373841559915	25.619552364879844	24.962918210041334
98-99	12.838733846884107	36.562360091471355	25.442581007703264	25.15632505394128
100-101	13.170737071990157	36.35019416049797	25.126489905995523	25.352578861516346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	4.5
5	8.5
6	12.5
7	17.0
8	27.5
9	48.0
10	96.0
11	215.5
12	390.0
13	657.5
14	1088.0
15	1671.5
16	2457.5
17	3376.0
18	4509.5
19	6159.5
20	8227.5
21	10853.5
22	14407.0
23	19048.0
24	24275.5
25	29976.0
26	36479.0
27	42834.0
28	48306.5
29	53013.0
30	56909.5
31	59387.0
32	60800.0
33	61147.0
34	59788.0
35	58023.5
36	55974.5
37	53906.0
38	52140.0
39	50947.5
40	50429.0
41	49989.0
42	49507.5
43	48278.0
44	46268.5
45	43545.5
46	39651.5
47	34770.5
48	29844.0
49	24940.0
50	20267.5
51	16322.5
52	12934.5
53	10275.0
54	8212.0
55	6584.0
56	5476.0
57	4608.0
58	3964.5
59	3505.0
60	3138.5
61	2829.0
62	2559.5
63	2353.0
64	2069.0
65	1734.0
66	1512.5
67	1299.5
68	1037.0
69	826.0
70	706.5
71	559.0
72	442.5
73	361.0
74	274.5
75	212.0
76	169.0
77	136.5
78	101.0
79	76.5
80	69.0
81	65.5
82	54.5
83	41.0
84	34.0
85	27.5
86	24.5
87	26.0
88	25.5
89	22.0
90	16.5
91	12.5
92	12.5
93	10.0
94	5.5
95	5.5
96	4.5
97	1.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0024123500972744703
2	4.2570884069549473E-4
3	0.004966603141447438
4	0.016035032999530303
5	2.1285442034774736E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.5475736724624565E-5
12-13	0.0
14-15	0.0
16-17	3.5475736724624565E-5
18-19	0.0013480779955357333
20-21	0.0
22-23	0.0
24-25	3.5475736724624565E-5
26-27	0.0
28-29	1.0642721017387368E-4
30-31	0.0
32-33	0.0010287963650141123
34-35	0.0031218648317669615
36-37	6.030875243186176E-4
38-39	4.2570884069549473E-4
40-41	7.095147344924913E-5
42-43	3.547573672462456E-4
44-45	0.01003963349306875
46-47	0.01858928604370327
48-49	0.004753748721099691
50-51	0.028735346746945892
52-53	0.0
54-55	1.0642721017387368E-4
56-57	0.0
58-59	0.0
60-61	0.005959923769736927
62-63	0.001454505205709607
64-65	0.004753748721099691
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.0642721017387368E-4
76-77	0.0028025832012453403
78-79	0.010217012176691874
80-81	0.0029444861481438384
82-83	0.011103905594807487
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.001454505205709607
92-93	0.009294643021851635
94-95	0.0
96-97	0.002093068466752849
98-99	7.804662079417404E-4
100-101	9.223691548402386E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1409414.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.51697085223434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.64605039215064	81.55102344437051
2	1.7398008966734848	2.906058015523412
3	0.2964626039496857	0.7427897595853019
4	0.09988921547835183	0.3336977879023227
5	0.061468771436754215	0.2566842796203029
6	0.017876117274277174	0.08957754992081576
7	0.024981351067010098	0.1460456738239041
8	0.018557003728768333	0.12398597916162792
9	0.009027070599780288	0.06785222329466208
>10	0.07488598789861593	1.1456289868490221
>50	0.004815934379585274	0.3109914713705434
>100	0.004244625011484422	0.6885087389712359
>500	8.892607910375733E-4	0.49674265728439926
>1k	7.880806498526808E-4	1.7886297943719123
>5k	1.7512594044527736E-4	1.1665688079557952
>10k+	8.756297022263868E-5	8.185214829994251
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	111927	7.9413855687541055	TruSeq Adapter, Index 1 (100% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	8403	0.5962052313940404	TruSeq Adapter, Index 1 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	7549	0.5356126730683817	TruSeq Adapter, Index 1 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT	4925	0.34943600673755193	TruSeq Adapter, Index 1 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	4108	0.2914686529295154	TruSeq Adapter, Index 1 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	4022	0.28536682621287995	TruSeq Adapter, Index 1 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	3369	0.2390355140505203	TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC	1922	0.1363687319694568	TruSeq Adapter, Index 1 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT	1551	0.11004573531978537	TruSeq Adapter, Index 1 (98% over 50bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT	1550	0.10997478384633613	TruSeq Adapter, Index 1 (98% over 50bp)
CACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	1549	0.1099038323728869	TruSeq Adapter, Index 1 (100% over 47bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	1462	0.10373105418280222	TruSeq Adapter, Index 1 (98% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.547573672462456E-4	0.0	0.0
2	4.966603141447439E-4	0.0	0.0
3	5.676117875939929E-4	0.0	0.0
4	7.804662079417403E-4	0.0	0.0
5	8.514176813909895E-4	0.0	0.0
6	0.0011352235751879859	0.0	0.0
7	0.0015609324158834805	0.0	0.0
8	0.0021994956769267227	0.0	0.0
9	0.002341398623825221	0.0	0.0
10-11	0.003618525145911705	0.0	0.0
12-13	0.005818020822838428	0.0	0.0
14-15	0.009188215811677761	0.0	0.0
16-17	0.011848896066024604	0.0	0.0
18-19	0.013658158638980455	0.0	0.0
20-21	0.015183615318139311	0.0	0.0
22-23	0.021037111877702366	0.0	0.0
24-25	0.032105541735785226	0.0	0.0
26-27	0.03799451403207291	0.0	0.0
28-29	0.040087582498825755	3.547573672462456E-5	0.0
30-31	0.041648514914709237	7.095147344924912E-5	0.0
32-33	0.044415622379229955	7.095147344924912E-5	0.0
34-35	0.046615118056156674	7.095147344924912E-5	0.0
36-37	0.0486727107861849	7.095147344924912E-5	0.0
38-39	0.05108506088345936	7.095147344924912E-5	0.0
40-41	0.054206925715226326	7.095147344924912E-5	0.0
42-43	0.05747069349389179	7.095147344924912E-5	0.0
44-45	0.062224442214991474	7.095147344924912E-5	0.0
46-47	0.06740389977678667	7.095147344924912E-5	0.0
48-49	0.0742507169646392	7.095147344924912E-5	0.0
50-51	0.08287132098872298	7.095147344924912E-5	7.095147344924912E-5
52-53	0.0924497699043716	7.095147344924912E-5	7.095147344924912E-5
54-55	0.10649816164732293	7.095147344924912E-5	7.095147344924912E-5
56-57	0.12381032116893971	7.095147344924912E-5	7.095147344924912E-5
58-59	0.1459117051483808	7.095147344924912E-5	1.0642721017387367E-4
60-61	0.17454062468515286	7.095147344924912E-5	1.4190294689849823E-4
62-63	0.21111610924824076	7.095147344924912E-5	1.4190294689849823E-4
64-65	0.260604761979092	7.095147344924912E-5	1.4190294689849823E-4
66-67	0.3264122536032706	7.095147344924912E-5	1.4190294689849823E-4
68-69	0.404991010448314	7.095147344924912E-5	1.4190294689849823E-4
70-71	0.5058840056931462	7.095147344924912E-5	1.4190294689849823E-4
72-73	0.6383858823596189	7.095147344924912E-5	1.4190294689849823E-4
74-75	0.8004390477177039	7.095147344924912E-5	1.4190294689849823E-4
76-77	1.0044954853577444	7.095147344924912E-5	1.4190294689849823E-4
78-79	1.2483556996028136	7.095147344924912E-5	1.4190294689849823E-4
80-81	1.53737652669833	7.095147344924912E-5	1.4190294689849823E-4
82-83	1.885180649546549	1.4190294689849823E-4	1.4190294689849823E-4
84-85	2.282579852335793	1.4190294689849823E-4	1.4190294689849823E-4
86-87	2.7463541585368105	1.4190294689849823E-4	1.4190294689849823E-4
88-89	3.2895586392642615	1.4190294689849823E-4	1.4190294689849823E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGGA	1070	0.0	85.68522	1
CCGGAAG	1250	0.0	83.987495	3
TCTGAAG	1755	0.0	82.01587	3
CTGAAGA	1975	0.0	81.77654	4
GACCGGA	1030	0.0	79.78868	1
ACCGGAA	1295	0.0	78.86805	2
TGAAGAG	2205	0.0	72.802795	5
GAGCACA	24985	0.0	72.29144	9
AGAGCAC	25090	0.0	72.026764	8
GAAGAGC	25260	0.0	71.54203	6
AAGAGCA	25555	0.0	70.753334	7
AGCGGAA	830	0.0	68.68083	2
GCGGAAG	830	0.0	68.68083	3
GAGCGGA	730	0.0	68.32801	1
TAAGAGC	460	0.0	68.15826	3
GGAAGAG	24335	0.0	67.76232	5
CGGAAGA	23680	0.0	67.40227	4
ACGGAAG	880	0.0	66.39798	3
CTCTGAA	1080	0.0	65.53826	2
ATCTGAA	1125	0.0	65.02803	2
>>END_MODULE