##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_CAGATC_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16000000
Sequences flagged as poor quality	0
Sequence length	101
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.2387554375	31.0	28.0	33.0	23.0	34.0
2	30.4430774375	31.0	30.0	33.0	26.0	34.0
3	27.6518910625	31.0	26.0	31.0	16.0	34.0
4	32.265564625	35.0	32.0	37.0	19.0	37.0
5	33.1053388125	35.0	33.0	37.0	26.0	37.0
6	34.3031335625	35.0	35.0	37.0	30.0	37.0
7	34.8170415	35.0	35.0	37.0	32.0	37.0
8	35.0118463125	35.0	35.0	37.0	32.0	37.0
9	36.582085375	38.0	35.0	39.0	32.0	39.0
10-11	36.744018906250005	39.0	36.0	39.0	32.5	39.0
12-13	36.7630855625	39.0	36.0	39.0	32.5	39.0
14-15	37.89782875	40.0	37.0	41.0	33.0	41.0
16-17	37.82768553125	40.0	37.0	41.0	32.5	41.0
18-19	37.84282853125	39.5	37.0	41.0	33.0	41.0
20-21	37.86241034375	40.0	37.0	41.0	32.5	41.0
22-23	37.948906875000006	40.0	37.0	41.0	33.0	41.0
24-25	37.896023562500005	40.0	37.0	41.0	33.0	41.0
26-27	37.6428869375	40.0	37.0	41.0	32.0	41.0
28-29	37.48722496875	39.5	36.5	41.0	31.5	41.0
30-31	37.3914601875	39.0	36.5	41.0	31.0	41.0
32-33	37.338014218750004	39.0	36.0	41.0	31.0	41.0
34-35	36.611381031250005	39.0	35.0	40.0	30.0	41.0
36-37	36.450773625	39.0	35.0	40.0	30.0	41.0
38-39	36.41475984375	39.0	35.0	40.0	30.0	41.0
40-41	36.882242968750006	39.0	35.5	40.0	30.5	41.0
42-43	36.835173781250006	39.0	36.0	40.0	30.0	41.0
44-45	36.6163435625	39.0	35.5	40.0	30.0	41.0
46-47	36.350911124999996	39.0	35.0	40.0	30.0	41.0
48-49	36.37843546875	39.0	35.0	40.0	29.5	41.0
50-51	36.19969890625	39.0	35.0	40.0	29.0	41.0
52-53	35.748011500000004	38.0	34.0	40.0	28.0	41.0
54-55	35.707048156249996	38.0	34.0	40.0	28.0	41.0
56-57	35.5534720625	38.0	34.0	40.0	28.0	41.0
58-59	35.140352875000005	37.5	34.0	40.0	27.5	41.0
60-61	34.815151	37.0	34.0	40.0	27.0	41.0
62-63	34.39650909375	36.5	33.5	39.5	26.5	40.5
64-65	33.34802715625	36.0	32.0	39.0	24.5	40.0
66-67	33.41151628125	36.0	33.0	39.0	26.0	40.0
68-69	31.90724075	35.0	32.0	37.5	14.5	40.0
70-71	28.43816246875	34.5	27.0	37.0	2.0	39.5
72-73	27.54491146875	34.0	25.0	36.5	2.0	39.0
74-75	27.198980875	34.0	24.5	36.0	2.0	39.0
76-77	24.7711874375	30.5	20.0	33.5	2.0	35.5
78-79	26.586955562500002	33.5	24.0	35.0	2.0	37.0
80-81	26.71575184375	34.0	24.5	35.0	2.0	37.0
82-83	26.591257218750002	34.0	24.0	35.0	2.0	36.0
84-85	26.43317140625	34.0	24.0	35.0	2.0	36.0
86-87	26.2743438125	34.0	23.5	35.0	2.0	36.0
88-89	26.08033834375	34.0	23.0	35.0	2.0	35.5
90-91	25.9367806875	34.0	21.0	35.0	2.0	35.0
92-93	25.76761646875	33.5	20.0	35.0	2.0	35.0
94-95	25.582798250000003	33.0	18.5	35.0	2.0	35.0
96-97	25.39163409375	33.0	13.5	35.0	2.0	35.0
98-99	25.126866749999998	33.0	2.0	35.0	2.0	35.0
100-101	23.836618	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1307	1	1.890583402261484
1307	2	1.1814895626686202
1307	3	2.371763406049027
1307	4	1.976981593514651
1307	5	2.3199243560593317
1307	6	1.14509364956632
1307	7	0.6251858892909681
1307	8	0.4323224524721354
1307	9	0.5129249128350963
1307	10-11	0.46338643875001395
1307	12-13	0.4557698303584914
1307	14-15	0.42523209832057063
1307	16-17	0.48844787954970315
1307	18-19	0.33344808775541424
1307	20-21	0.5473593017312339
1307	22-23	0.2920523256213272
1307	24-25	0.39634287608341623
1307	26-27	0.3502606918526254
1307	28-29	0.5634854577895752
1307	30-31	0.5733936018920005
1307	32-33	0.516020510211618
1307	34-35	0.6795467445317911
1307	36-37	0.6649469971447957
1307	38-39	0.6920012751091207
1307	40-41	0.5563013717112781
1307	42-43	0.373945569527109
1307	44-45	0.4057094028422341
1307	46-47	0.35684292942532636
1307	48-49	0.35018039321523986
1307	50-51	0.2579156437019847
1307	52-53	0.25829928361433474
1307	54-55	0.0318254761667518
1307	56-57	-0.03413485915128689
1307	58-59	-0.14765198841968186
1307	60-61	-0.20498928818272333
1307	62-63	-0.23682694673117766
1307	64-65	-0.9728101161600193
1307	66-67	-0.6730924469348594
1307	68-69	-1.088850352024032
1307	70-71	-0.3251717213495411
1307	72-73	-0.06474719179650279
1307	74-75	-0.038473415463695204
1307	76-77	-0.6211371804992041
1307	78-79	-0.18790170921718996
1307	80-81	-0.0923308008914887
1307	82-83	-0.13867496655192824
1307	84-85	-0.13127540768143575
1307	86-87	-0.13378967941691755
1307	88-89	-0.174525629771356
1307	90-91	-0.19781416793420092
1307	92-93	-0.206459134594418
1307	94-95	-0.2007831815789558
1307	96-97	-0.214696170541135
1307	98-99	-0.24444755560520548
1307	100-101	-0.6400243293682024
1306	1	2.068552421636433
1306	2	1.3587155947971041
1306	3	3.255141854435667
1306	4	2.430075741630702
1306	5	2.5139034602184864
1306	6	1.3300629585815216
1306	7	0.7517958678518113
1306	8	0.4998305779290817
1306	9	0.5575940923070846
1306	10-11	0.5314247059410846
1306	12-13	0.5387602168698038
1306	14-15	0.44739996143756144
1306	16-17	0.5644216322850966
1306	18-19	0.5478414595467243
1306	20-21	0.7339216051057491
1306	22-23	0.5800530947177833
1306	24-25	0.5748669721023987
1306	26-27	0.5859826971583999
1306	28-29	0.661377579400714
1306	30-31	0.7111435293805499
1306	32-33	0.6599561921673214
1306	34-35	1.0011834460070261
1306	36-37	1.1077527633675288
1306	38-39	1.045723892309681
1306	40-41	0.8894507914224192
1306	42-43	0.7404424570852157
1306	44-45	0.8405832032158855
1306	46-47	0.6647712396863454
1306	48-49	0.7567457383122331
1306	50-51	0.7671349642333922
1306	52-53	0.9263529294617001
1306	54-55	0.8449642779527125
1306	56-57	0.6860332101741733
1306	58-59	0.6211201071262167
1306	60-61	0.6309521665996698
1306	62-63	0.6246048798793424
1306	64-65	0.018367533278571102
1306	66-67	0.3762955574279232
1306	68-69	0.23276488407713458
1306	70-71	-0.12919572161565895
1306	72-73	-0.15051918840745415
1306	74-75	-0.16165863343523057
1306	76-77	-0.0864397825269414
1306	78-79	-0.12683046353823713
1306	80-81	-0.18099035775716033
1306	82-83	-0.2002642790695397
1306	84-85	-0.1343582846974023
1306	86-87	-0.09999754891120105
1306	88-89	-0.18468255243471177
1306	90-91	-0.16892620340900422
1306	92-93	-0.20511696814700642
1306	94-95	-0.18141978167885497
1306	96-97	-0.08920531241592045
1306	98-99	-0.11673655454443477
1306	100-101	-0.2517738600568258
1101	1	0.8907640227232712
1101	2	0.413226936289508
1101	3	1.215352512154908
1101	4	-1.1313832086729434
1101	5	0.19970643330889004
1101	6	0.16205274724431007
1101	7	0.16003214123981024
1101	8	0.1486853945200579
1101	9	0.23934414695924744
1101	10-11	0.2519827334400233
1101	12-13	0.19656341205067207
1101	14-15	0.26613862426873425
1101	16-17	0.2586937374172251
1101	18-19	0.2556357405262162
1101	20-21	0.31147045235564264
1101	22-23	0.3011094071931524
1101	24-25	0.30309284243000434
1101	26-27	0.3275952194023546
1101	28-29	0.4120029357184052
1101	30-31	0.3484557495603582
1101	32-33	0.3248542768287166
1101	34-35	0.4813828215936695
1101	36-37	0.4985161373958391
1101	38-39	0.4088278185950571
1101	40-41	0.34809066125455246
1101	42-43	0.24295262605539136
1101	44-45	0.25359723979147475
1101	46-47	0.2519745651106149
1101	48-49	0.24080951926877248
1101	50-51	0.17242839318767267
1101	52-53	0.22325857770487545
1101	54-55	0.16034809541577033
1101	56-57	0.10666275660442182
1101	58-59	0.0543437969311924
1101	60-61	-0.06928842027970461
1101	62-63	0.018736946188568027
1101	64-65	-0.485154753628386
1101	66-67	-0.345452908289559
1101	68-69	-0.520695004855952
1101	70-71	-0.1638121726483206
1101	72-73	0.08513923455768335
1101	74-75	0.1389458717491685
1101	76-77	-0.09962598551988577
1101	78-79	0.04759530793844036
1101	80-81	0.06262975183116026
1101	82-83	0.005277840094073838
1101	84-85	-0.04950452939677419
1101	86-87	0.04639377927444244
1101	88-89	-0.02690878984047984
1101	90-91	0.02167720101474302
1101	92-93	-0.0657539740918267
1101	94-95	-0.127886860474959
1101	96-97	-0.08868515939662203
1101	98-99	-0.11148200147268028
1101	100-101	-0.3162906438281823
1305	1	0.3840635154047831
1305	2	0.030942446844893112
1305	3	0.42554030782275376
1305	4	-0.9801231019013699
1305	5	0.621360063114949
1305	6	0.2891249908818736
1305	7	0.11435029468367475
1305	8	0.14307817616210627
1305	9	0.1477808611899576
1305	10-11	0.15937801175410016
1305	12-13	0.1693115338771065
1305	14-15	0.17418338554995216
1305	16-17	0.17586883641561002
1305	18-19	0.1718195766640207
1305	20-21	0.25404064029206097
1305	22-23	0.11849774654466216
1305	24-25	0.19730274921319335
1305	26-27	0.18895851038142553
1305	28-29	0.24400631323332078
1305	30-31	0.19975262739431088
1305	32-33	0.17065919761157744
1305	34-35	0.3170351414778523
1305	36-37	0.3476725598770187
1305	38-39	0.37105142892940535
1305	40-41	0.22164040399736962
1305	42-43	0.07954659596449432
1305	44-45	0.06733353132122488
1305	46-47	0.11202277506551184
1305	48-49	0.23060310590147282
1305	50-51	0.16547772793450122
1305	52-53	0.1880927019731189
1305	54-55	0.06700821205556906
1305	56-57	0.05305721704117161
1305	58-59	0.03766255012465791
1305	60-61	-0.024071174532537043
1305	62-63	-0.012428750867890415
1305	64-65	-0.38968436556089614
1305	66-67	-0.24648835388797607
1305	68-69	-0.5777725352503182
1305	70-71	0.1994600528405286
1305	72-73	0.44332945470858576
1305	74-75	0.4005097752287803
1305	76-77	0.18827122523607898
1305	78-79	0.3756192871167805
1305	80-81	0.48728200542214495
1305	82-83	0.4757443236206491
1305	84-85	0.456120201235354
1305	86-87	0.43119187597088526
1305	88-89	0.4089030752916898
1305	90-91	0.3777624455403057
1305	92-93	0.3969760953460266
1305	94-95	0.33476923125331126
1305	96-97	0.361825923410521
1305	98-99	0.3783393978807652
1305	100-101	0.15133047861289128
1102	1	1.7369858666325584
1102	2	0.922335604773636
1102	3	1.8108434548590537
1102	4	2.678234681820875
1102	5	2.2664872754155994
1102	6	0.9200974964312607
1102	7	0.4027677154439431
1102	8	0.279994283934613
1102	9	0.13972186234329342
1102	10-11	0.04989190688002765
1102	12-13	0.04649292982615805
1102	14-15	0.11658550989375982
1102	16-17	0.09554820334999903
1102	18-19	0.10545337992859061
1102	20-21	0.14920701902126154
1102	22-23	0.0034713745817214203
1102	24-25	-0.03129318602066178
1102	26-27	-0.024321792715568336
1102	28-29	-0.010405346369047663
1102	30-31	0.12591659857646675
1102	32-33	0.19457256423261526
1102	34-35	0.1872219854738688
1102	36-37	-0.02661771779720823
1102	38-39	0.029798937045384832
1102	40-41	0.062316440632308456
1102	42-43	-0.06822196833022076
1102	44-45	0.11544257589675055
1102	46-47	0.023835311762780975
1102	48-49	-0.009722683272080701
1102	50-51	-0.10171685392332819
1102	52-53	0.038632852565513076
1102	54-55	-0.07970653613374168
1102	56-57	-0.02522991069476177
1102	58-59	-0.06320552272865143
1102	60-61	-0.3055321615301949
1102	62-63	-0.1650818634213067
1102	64-65	-0.6271902863647796
1102	66-67	-0.39603471125911227
1102	68-69	-0.648071258328013
1102	70-71	-0.024454410356916867
1102	72-73	0.3875659267107281
1102	74-75	0.41137840690415217
1102	76-77	0.14705463222157533
1102	78-79	0.3551408214580718
1102	80-81	0.4106295343165627
1102	82-83	0.45973890991203703
1102	84-85	0.47629469722253504
1102	86-87	0.44332982618419337
1102	88-89	0.41538133892544593
1102	90-91	0.4012047458825734
1102	92-93	0.41969629288586674
1102	94-95	0.39585119873994046
1102	96-97	0.28817565292777303
1102	98-99	0.23531425161922925
1102	100-101	0.07204595003259584
1304	1	1.5031977904450748
1304	2	0.7986266807393854
1304	3	0.9921425679455638
1304	4	2.410126068762729
1304	5	1.7276286244985926
1304	6	0.7168597150497646
1304	7	0.3740965303908368
1304	8	0.2499951668878424
1304	9	0.1263657015056836
1304	10-11	0.049085017658718755
1304	12-13	0.013326451371561632
1304	14-15	-0.06435375407047417
1304	16-17	-0.039215735416213704
1304	18-19	0.059516786763623486
1304	20-21	0.15685743269453667
1304	22-23	-0.001296657192085604
1304	24-25	0.047778913961131764
1304	26-27	0.0325852463111147
1304	28-29	-0.08395633461618246
1304	30-31	-0.009247184827444244
1304	32-33	0.009756652908471608
1304	34-35	0.09922681199670791
1304	36-37	0.12916892515978873
1304	38-39	0.12040424932741445
1304	40-41	0.11084899374331059
1304	42-43	0.0062997584591002465
1304	44-45	0.09028483183531932
1304	46-47	-0.05155427988995598
1304	48-49	-0.047688893728569326
1304	50-51	-0.08254345686673759
1304	52-53	-0.06654464926089076
1304	54-55	-0.05017462722101129
1304	56-57	-0.10226428108030916
1304	58-59	-0.20624222555939298
1304	60-61	-0.1890568271024904
1304	62-63	-0.2100408979475077
1304	64-65	-0.12342269751495394
1304	66-67	-0.19224642585977847
1304	68-69	-0.20574912954152325
1304	70-71	0.2191449531439602
1304	72-73	0.4863018550856566
1304	74-75	0.5689937493502164
1304	76-77	0.3002509564863516
1304	78-79	0.5562679004201492
1304	80-81	0.5832622178016891
1304	82-83	0.569234441570746
1304	84-85	0.5993580583053699
1304	86-87	0.5146776481696875
1304	88-89	0.5132415942409132
1304	90-91	0.5900190593770382
1304	92-93	0.586137153857873
1304	94-95	0.5900625057426296
1304	96-97	0.5475270671287866
1304	98-99	0.5334698443777555
1304	100-101	0.49599466001808423
1103	1	0.8745262799115849
1103	2	0.4797140408176084
1103	3	-0.5765955227545057
1103	4	-0.3907829243277803
1103	5	1.1678180969517555
1103	6	0.4802551940296951
1103	7	0.2175780957900102
1103	8	0.09226663475691765
1103	9	0.03232250375116763
1103	10-11	0.0408127613277145
1103	12-13	-0.07132104126735328
1103	14-15	-0.07654679207256265
1103	16-17	-0.07115664688844703
1103	18-19	0.023476245097427295
1103	20-21	0.027887531329234605
1103	22-23	-0.13365292857462663
1103	24-25	-0.07615285348819611
1103	26-27	-0.13977378546420738
1103	28-29	-0.1411546357534732
1103	30-31	-7.870276206034532E-4
1103	32-33	-0.05147036436144958
1103	34-35	-0.1220435333554164
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1213	10-11	-0.027579131836844795
1213	12-13	0.01660872601492258
1213	14-15	-0.15689286782556877
1213	16-17	-0.06768482947403953
1213	18-19	-0.0719831861503124
1213	20-21	0.02498418201620467
1213	22-23	0.06355915393851319
1213	24-25	0.017366178671750276
1213	26-27	0.10592612465884343
1213	28-29	-0.038829481848232206
1213	30-31	-0.037541156364888195
1213	32-33	0.03863916754232122
1213	34-35	-0.12346784656556054
1213	36-37	0.004319370125898558
1213	38-39	-0.08676026668882741
1213	40-41	0.025507586314347463
1213	42-43	0.07743377512306182
1213	44-45	0.02133363758910889
1213	46-47	0.05269851648797186
1213	48-49	-0.04538883007138139
1213	50-51	-0.040353578100152276
1213	52-53	-0.1313476106901348
1213	54-55	0.06680893736424309
1213	56-57	0.1406731581723335
1213	58-59	0.07042097015518323
1213	60-61	0.1560796341256605
1213	62-63	0.19620303215764068
1213	64-65	0.5084054059453962
1213	66-67	0.45723882192131526
1213	68-69	0.4351320774885039
1213	70-71	-0.687642824594846
1213	72-73	-0.6837613259522008
1213	74-75	-0.6243976179777135
1213	76-77	-0.46592606615504195
1213	78-79	-0.5837744118582435
1213	80-81	-0.6032988470142513
1213	82-83	-0.6009934036839795
1213	84-85	-0.5774418303911979
1213	86-87	-0.5695023135346489
1213	88-89	-0.624876718534761
1213	90-91	-0.6184127727315349
1213	92-93	-0.5342416683358877
1213	94-95	-0.5343129259454429
1213	96-97	-0.4816309706449289
1213	98-99	-0.46387614343716166
1213	100-101	-0.35021690847063525
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1071.0
3	210.0
4	274.0
5	490.0
6	1146.0
7	2805.0
8	5659.0
9	9108.0
10	13573.0
11	18875.0
12	26419.0
13	35279.0
14	40936.0
15	45110.0
16	52179.0
17	62839.0
18	80022.0
19	103553.0
20	135000.0
21	171936.0
22	218113.0
23	282106.0
24	377063.0
25	542343.0
26	749709.0
27	922008.0
28	319705.0
29	326610.0
30	404357.0
31	504132.0
32	631161.0
33	808297.0
34	1062580.0
35	1443098.0
36	2076800.0
37	2754488.0
38	1643375.0
39	127551.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.409331799150785	21.284744940066982	10.830577810274065	38.47534545050817
2	15.389573410660203	40.17619811033531	19.25327098580323	25.180957493201262
3	10.3834812721341	14.827670432961154	41.96887878810227	32.819969506802465
4	13.723843691420837	16.934754653811463	11.671552671995942	57.66984898277177
5	34.94612382342447	20.114895509848825	14.272850835775582	30.66612983095112
6	33.37820002523751	24.86957023913326	16.8969907545573	24.855238981071928
7	8.24868125	48.8357875	22.33226875	20.5832625
8	8.604538037783627	49.67260935453808	19.260482453780153	22.462370153898135
9	25.952956249999996	35.08604375	20.04135625	18.919643750000002
10-11	16.999641749910438	42.17922929480732	21.848508587127146	18.97262036815509
12-13	7.582446875	44.7331	19.5217	28.162753125000002
14-15	8.40391074104411	41.33611871085758	19.612877149169915	30.647093398928394
16-17	17.214083582305037	33.71610366327077	19.703418332932028	29.366394421492153
18-19	10.989077163606295	29.14827938686116	29.045226036431952	30.81741741310059
20-21	21.808384375	29.15576875	29.017500000000002	20.018346875000002
22-23	7.326741082921318	49.471849966990625	20.346645021665314	22.854763928422745
24-25	7.471296875	31.5848	29.01885	31.925053125
26-27	8.04773613544228	31.1674724428846	22.68682683774337	38.09796458392975
28-29	16.689338547334636	40.31307570326892	19.527564256891065	23.47002149250537
30-31	7.519432424911317	28.584648216351283	31.362770525432893	32.5331488333045
32-33	8.07561875	40.789271875	20.626715625	30.50839375
34-35	7.7921320208836855	40.435355015250515	21.174442788224354	30.598070175641446
36-37	16.559299879212364	40.36155165674561	20.85167345813264	22.22747500590938
38-39	7.671979563496169	30.82414015452628	30.404311950808488	31.099568331169063
40-41	7.715765016001483	39.54671095771177	30.46940505684293	22.268118969443822
42-43	8.155381142676903	30.42366728795564	30.699617473040714	30.721334096326743
44-45	16.902871513270163	30.867341796599412	21.58633501764235	30.64345167248807
46-47	8.407784002508851	39.14763113069732	29.913066794421837	22.53151807237199
48-49	17.389203459179544	30.425182040900427	29.913630536591924	22.271983963328104
50-51	7.811221637224524	30.256158895554574	21.56369522231135	40.36892424490956
52-53	16.66642551784158	30.267838010365317	31.210390328905017	21.855346142888084
54-55	7.614699005355958	30.398051681064775	30.955991478542185	31.031257835037085
56-57	8.098146476147166	30.247279014583967	30.326642000682252	31.327932508586613
58-59	16.837715624999998	30.394315625	31.148671875	21.619296875
60-61	8.172803891200363	31.327137311919124	30.247434085696945	30.252624711183568
62-63	16.703684388881644	32.03479951304982	29.938479976929994	21.323036121138543
64-65	7.6542687419427216	49.80247527241662	20.925180417584603	21.618075568056053
66-67	7.603428239141487	50.19391017487948	20.950998922870557	21.251662663108487
68-69	7.6685986669363775	49.72503129157933	21.124519970001085	21.48185007148321
70-71	8.003388750529492	47.816501221328316	21.685719013393594	22.494391014748597
72-73	8.585784375	45.303278125	22.554453125000002	23.556484375
74-75	9.094562789228924	43.422836063363214	22.909013465800186	24.573587681607677
76-77	9.311754188124949	41.837560769277765	23.59666621925832	25.254018823338964
78-79	9.68591241830294	41.01586728570139	23.885713117854515	25.41250717814115
80-81	10.185777736609952	39.825863216519416	24.0965604717983	25.891798575072333
82-83	10.368106693030501	39.25355954430508	24.033157381026406	26.345176381638012
84-85	10.736245667474005	38.74703560721364	24.26159556957342	26.255123155738936
86-87	10.912027677062273	38.62400252900569	24.15285434392227	26.311115450009765
88-89	11.517718144500844	37.5937613486353	24.109127148871565	26.779393357992298
90-91	11.686015990187999	37.89748243429633	23.965788248930885	26.45071332658479
92-93	12.024798140498838	37.54223596389748	23.84067427542142	26.592291620182262
94-95	12.383099824649738	37.41676862515293	23.556360334540503	26.643771215656827
96-97	12.699599574267575	37.06599008820216	23.601761555717125	26.632648781813135
98-99	13.119844579694014	36.82338187103306	23.22889260688232	26.827880942390603
100-101	13.789682180055344	36.329809834039324	22.881953502709468	26.998554483195868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	1.0
3	0.5
4	1.5
5	2.0
6	1.0
7	2.5
8	3.0
9	7.0
10	16.5
11	27.0
12	48.5
13	116.5
14	284.5
15	604.5
16	1117.0
17	1976.0
18	3320.0
19	5400.5
20	8810.5
21	14894.0
22	26058.0
23	45495.0
24	77521.5
25	126351.5
26	193615.0
27	276686.0
28	368792.0
29	461270.0
30	544238.5
31	611140.0
32	665154.5
33	703939.0
34	720823.0
35	721402.0
36	711936.5
37	698531.5
38	690990.5
39	690225.0
40	698661.5
41	713980.0
42	723800.0
43	720106.0
44	697125.0
45	654813.0
46	596840.5
47	525229.5
48	446345.5
49	374476.0
50	319244.5
51	267774.0
52	204869.0
53	147311.0
54	108696.5
55	81840.5
56	62644.0
57	48749.5
58	38466.5
59	31113.0
60	25532.5
61	21141.5
62	18030.0
63	15603.5
64	13421.5
65	11542.0
66	9935.0
67	8611.5
68	7603.5
69	6584.0
70	5632.0
71	4887.5
72	4111.5
73	3357.5
74	2732.0
75	2158.0
76	1656.0
77	1263.0
78	938.5
79	685.0
80	477.0
81	350.0
82	250.5
83	164.5
84	116.5
85	92.0
86	62.5
87	42.5
88	35.0
89	28.0
90	21.5
91	12.0
92	9.5
93	6.5
94	4.5
95	4.5
96	3.5
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022762499999999998
2	0.00673125
3	0.0022875
4	0.018443750000000002
5	4.4375E-4
6	5.625E-5
7	0.0
8	6.2499999999999995E-6
9	0.0
10-11	2.4999999999999998E-5
12-13	0.0
14-15	9.0625E-5
16-17	1.4062500000000002E-4
18-19	2.1874999999999998E-4
20-21	0.0
22-23	6.2499999999999995E-6
24-25	0.0
26-27	2.1875E-5
28-29	2.4999999999999998E-5
30-31	1.5625E-5
32-33	0.0
34-35	2.90625E-4
36-37	1.125E-4
38-39	1.8750000000000002E-5
40-41	4.7812500000000003E-4
42-43	0.004596875
44-45	0.0053593749999999996
46-47	0.004046875
48-49	0.022896875
50-51	0.0186
52-53	0.001240625
54-55	9.718750000000001E-4
56-57	6.125E-4
58-59	0.0
60-61	9.375000000000001E-6
62-63	3.7500000000000003E-5
64-65	6.531250000000001E-4
66-67	4.1250000000000005E-4
68-69	5.53125E-4
70-71	1.5625E-5
72-73	0.0
74-75	1.4062500000000002E-4
76-77	0.002796875
78-79	2.25E-4
80-81	0.00281875
82-83	0.0019031249999999999
84-85	6.000000000000001E-4
86-87	2.25E-4
88-89	2.1875E-5
90-91	3.1249999999999997E-6
92-93	6.25E-5
94-95	1.5000000000000001E-4
96-97	0.00174375
98-99	1.96875E-4
100-101	0.0011625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.08010085651243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.39526093282464	51.04240729435987
2	8.411081775192718	9.938551191815186
3	2.1323970643118515	3.7794670087702564
4	0.9737882800328344	2.3012603918891847
5	0.500961089037815	1.4798415832771206
6	0.38859752562869826	1.3775028604040798
7	0.24864710394475656	1.028306718511516
8	0.13058029848664074	0.6171757763571386
9	0.12965331375846342	0.6893937767907962
>10	0.6502851559470404	6.5063778753316
>50	0.023932443134982478	0.9542166321434851
>100	0.012417294712468158	1.3860654593844168
>500	0.0014552205411149832	0.630355563295552
>1k	7.135414994692447E-4	0.9009984145184955
>5k	1.2092172251229172E-4	0.5115624616709299
>10k+	1.0803922388609426E-4	16.856516991480323
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	2323335	14.520843750000001	TruSeq Adapter, Index 7 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT	126859	0.79286875	TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	48330	0.3020625	TruSeq Adapter, Index 7 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	34956	0.218475	TruSeq Adapter, Index 7 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	23411	0.14631875	TruSeq Adapter, Index 7 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT	23375	0.14609375	TruSeq Adapter, Index 7 (100% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	20483	0.12801875000000001	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.00114375	6.25E-6	6.25E-6
2	0.0016375	6.25E-6	6.25E-6
3	0.001775	6.25E-6	6.25E-6
4	0.0019625	6.25E-6	6.25E-6
5	0.00219375	6.25E-6	1.875E-5
6	0.0024375	6.25E-6	1.875E-5
7	0.00276875	6.25E-6	1.875E-5
8	0.00359375	1.25E-5	1.875E-5
9	0.0038625	1.25E-5	2.5E-5
10-11	0.006190625	1.25E-5	2.5E-5
12-13	0.00946875	1.25E-5	2.5E-5
14-15	0.014671875000000001	1.875E-5	2.8125E-5
16-17	0.017687500000000002	1.875E-5	3.125E-5
18-19	0.01915625	1.875E-5	3.125E-5
20-21	0.020903125	1.875E-5	3.125E-5
22-23	0.029725	1.875E-5	3.75E-5
24-25	0.047703125	1.875E-5	4.375E-5
26-27	0.055224999999999996	1.875E-5	4.375E-5
28-29	0.057278125	1.875E-5	4.375E-5
30-31	0.058937500000000004	1.875E-5	4.375E-5
32-33	0.061328125	1.875E-5	4.6875E-5
34-35	0.063621875	1.875E-5	5.0E-5
36-37	0.0663125	1.875E-5	5.0E-5
38-39	0.069484375	2.5E-5	5.0E-5
40-41	0.07360625	2.5E-5	5.0E-5
42-43	0.078628125	2.5E-5	5.625E-5
44-45	0.085603125	2.5E-5	5.625E-5
46-47	0.09451562499999999	2.5E-5	5.625E-5
48-49	0.10643749999999999	3.125E-5	5.625E-5
50-51	0.12260312500000001	3.125E-5	5.625E-5
52-53	0.143578125	3.75E-5	5.625E-5
54-55	0.170140625	3.75E-5	5.625E-5
56-57	0.20421250000000002	3.75E-5	5.625E-5
58-59	0.24967499999999998	3.75E-5	5.625E-5
60-61	0.308221875	3.75E-5	5.625E-5
62-63	0.38176875	3.75E-5	5.625E-5
64-65	0.478140625	4.375E-5	5.625E-5
66-67	0.597053125	4.375E-5	5.625E-5
68-69	0.745378125	4.375E-5	5.625E-5
70-71	0.92900625	4.375E-5	6.25E-5
72-73	1.15350625	4.375E-5	6.25E-5
74-75	1.427834375	5.625E-5	6.562500000000001E-5
76-77	1.75839375	6.25E-5	6.875E-5
78-79	2.14964375	6.25E-5	6.875E-5
80-81	2.60598125	6.25E-5	7.5E-5
82-83	3.147621875	6.25E-5	7.5E-5
84-85	3.78293125	6.875E-5	7.5E-5
86-87	4.52235625	6.875E-5	7.812499999999999E-5
88-89	5.375090625	6.875E-5	8.125E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGA	14910	0.0	83.50591	1
CCGGAAG	15370	0.0	83.27152	3
ACCGGAA	16100	0.0	79.58861	2
GAGCACA	393900	0.0	71.03844	9
AGAGCAC	398925	0.0	70.19124	8
TCCGAAG	4980	0.0	69.06881	3
AAGAGCA	406500	0.0	68.960365	7
GAAGAGC	408535	0.0	68.6417	6
GGAAGAG	412425	0.0	66.905	5
CGAAGAG	6215	0.0	66.25983	5
CGGAAGA	410800	0.0	65.757805	4
TCGGAAG	384355	0.0	65.252945	3
ATCGGAA	394880	0.0	63.553185	2
ACGGAAG	6870	0.0	63.137413	3
GATCGGA	401835	0.0	61.86412	1
GATTGGA	9090	0.0	60.225742	1
CCGAAGA	6800	0.0	60.083164	4
TTGGAAG	9120	0.0	59.69838	3
AATCATA	132210	0.0	58.645485	5
ATTGGAA	9460	0.0	57.806946	2
>>END_MODULE