##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2112_lane1_CAGATC_L001_R1_002.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.006278 31.0 26.0 31.0 16.0 34.0 2 29.85455175 31.0 30.0 33.0 25.0 34.0 3 26.3654041875 30.0 23.0 31.0 10.0 34.0 4 30.8096378125 35.0 28.0 35.0 19.0 37.0 5 32.1753090625 35.0 32.0 37.0 19.0 37.0 6 33.931046125 35.0 33.0 37.0 29.0 37.0 7 34.73878425 35.0 35.0 37.0 32.0 37.0 8 35.0150495625 35.0 35.0 37.0 32.0 37.0 9 36.638708125 39.0 35.0 39.0 32.0 39.0 10-11 36.74976328125 39.0 36.0 39.0 32.5 39.0 12-13 36.85525271875 39.0 37.0 39.0 33.0 39.0 14-15 38.1017284375 40.0 37.0 41.0 33.0 41.0 16-17 38.063835749999996 40.0 37.0 41.0 33.0 41.0 18-19 38.1123555625 40.0 37.0 41.0 33.0 41.0 20-21 38.12668884375 40.0 37.0 41.0 33.0 41.0 22-23 38.18197153125 40.0 37.0 41.0 33.0 41.0 24-25 38.1088035 40.0 37.0 41.0 33.0 41.0 26-27 37.87636971875 40.0 37.0 41.0 33.0 41.0 28-29 37.65559990625 40.0 37.0 41.0 32.5 41.0 30-31 37.57655046875 40.0 37.0 41.0 32.0 41.0 32-33 37.377778218749995 40.0 37.0 41.0 31.0 41.0 34-35 36.74274134375 39.0 36.0 41.0 30.0 41.0 36-37 36.44501365625 39.0 35.5 41.0 30.0 41.0 38-39 36.45351678125 39.0 35.0 41.0 30.0 41.0 40-41 37.12482053125 39.0 36.0 41.0 30.5 41.0 42-43 37.1421034375 40.0 36.0 41.0 31.0 41.0 44-45 37.02679684375 40.0 36.0 41.0 30.5 41.0 46-47 36.771677999999994 39.0 36.0 40.5 30.0 41.0 48-49 36.77964703124999 39.0 35.5 41.0 30.0 41.0 50-51 36.68429078125 39.0 35.5 41.0 30.0 41.0 52-53 36.26948928125 39.0 35.0 40.5 29.0 41.0 54-55 36.205499625 39.0 35.0 40.0 29.0 41.0 56-57 36.045969687500005 38.5 35.0 40.0 29.0 41.0 58-59 35.6638768125 38.0 34.0 40.0 28.0 41.0 60-61 35.39189475 38.0 34.0 40.0 28.0 41.0 62-63 34.980205749999996 37.0 34.0 39.5 28.0 41.0 64-65 34.281326843749994 36.0 33.0 39.0 27.0 40.5 66-67 34.2010628125 36.0 34.0 39.0 27.5 40.0 68-69 32.84661678125 35.5 32.5 37.5 23.5 40.0 70-71 28.98910984375 35.0 28.0 37.0 2.0 39.5 72-73 27.52014628125 34.5 24.0 36.5 2.0 39.0 74-75 27.1399105 34.0 22.5 36.0 2.0 39.0 76-77 25.3405094375 32.0 20.5 34.5 2.0 36.5 78-79 26.63624121875 34.0 23.5 35.0 2.0 37.0 80-81 26.66577246875 34.0 24.0 35.0 2.0 37.0 82-83 26.4989869375 34.0 23.0 35.0 2.0 36.0 84-85 26.3443404375 34.0 23.0 35.0 2.0 36.0 86-87 26.198771781250002 34.0 22.5 35.0 2.0 36.0 88-89 26.025453312499998 34.0 20.0 35.0 2.0 36.0 90-91 25.8866064375 34.0 19.5 35.0 2.0 35.0 92-93 25.7395011875 34.0 18.5 35.0 2.0 35.0 94-95 25.576915562499998 34.0 16.0 35.0 2.0 35.0 96-97 25.39709521875 34.0 5.0 35.0 2.0 35.0 98-99 25.183930625000002 33.5 2.0 35.0 2.0 35.0 100-101 24.190289406250002 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.8469818160176459 2201 2 0.4713029031854177 2201 3 -2.0006110939434762 2201 4 0.6170057316205266 2201 5 -2.8721922632801657 2201 6 -1.0769541055884986 2201 7 -0.6345366246056301 2201 8 -0.3232085525129733 2201 9 -0.22780772174170494 2201 10-11 -0.1491515738728424 2201 12-13 -0.13577995456314085 2201 14-15 -0.2737622263232069 2201 16-17 -0.2905478070272878 2201 18-19 -0.2778925805123933 2201 20-21 -0.40219119987231977 2201 22-23 -0.2986653897707541 2201 24-25 -0.2608041763057827 2201 26-27 -0.3380965877007114 2201 28-29 -0.3072312093822447 2201 30-31 -0.3103193334159329 2201 32-33 -0.11210495093835249 2201 34-35 -0.2893539437976429 2201 36-37 -0.18778332092446703 2201 38-39 -0.27737102985690854 2201 40-41 -0.3021790768898711 2201 42-43 -0.24880388307081347 2201 44-45 -0.3105754568302075 2201 46-47 -0.3766633114960314 2201 48-49 -0.30144603094366573 2201 50-51 -0.260587354518492 2201 52-53 -0.23490516243357717 2201 54-55 -0.22275747599656626 2201 56-57 -0.24280681214493427 2201 58-59 -0.20211797076552784 2201 60-61 -0.19068741813911316 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2214 40-41 0.11552211029798798 2214 42-43 0.14866337470662216 2214 44-45 0.18479952028269508 2214 46-47 0.20199762993426873 2214 48-49 0.24203453680437548 2214 50-51 0.27202286587833413 2214 52-53 0.263851795589396 2214 54-55 0.3061284749460853 2214 56-57 0.320135154981557 2214 58-59 0.4054320092397532 2214 60-61 0.37023268759470795 2214 62-63 0.37074458402383925 2214 64-65 0.5717348574080319 2214 66-67 0.47057696846474073 2214 68-69 0.5563382018713128 2214 70-71 -0.05483448907879662 2214 72-73 -0.5190170999480088 2214 74-75 -0.4283647063568665 2214 76-77 -0.16468544412749253 2214 78-79 -0.42246932202690246 2214 80-81 -0.4168194397180436 2214 82-83 -0.3747881599834564 2214 84-85 -0.3930836947903238 2214 86-87 -0.3980095386567122 2214 88-89 -0.3743540648918433 2214 90-91 -0.3573672908994858 2214 92-93 -0.3112809226813944 2214 94-95 -0.29929044471249355 2214 96-97 -0.29156470169119686 2214 98-99 -0.26004277237192497 2214 100-101 -0.10161324588662524 2213 1 -2.104987343731054 2213 2 -2.3081316037062365 2213 3 0.23739065718688934 2213 4 -1.8960793671201976 2213 5 0.3726380353148393 2213 6 0.1335498560896724 2213 7 -0.036094056055951285 2213 8 -0.12516788972295956 2213 9 -0.040659825874293176 2213 10-11 0.08214193617547494 2213 12-13 -0.09475396484856446 2213 14-15 -0.03817399508152164 2213 16-17 0.02572658087417068 2213 18-19 -0.04838353432151621 2213 20-21 -0.18046115850992095 2213 22-23 -0.23765837889094144 2213 24-25 -0.10480703337808706 2213 26-27 0.04529585513022738 2213 28-29 -0.048301853429002506 2213 30-31 0.029524869458697367 2213 32-33 0.16266210939100034 2213 34-35 -0.2402313884046734 2213 36-37 -0.07430520103068972 2213 38-39 -0.0832451462644741 2213 40-41 -0.13961485098734272 2213 42-43 -0.05503935448731312 2213 44-45 -0.030807491626184458 2213 46-47 -0.006244821226985664 2213 48-49 -0.05443998619480794 2213 50-51 0.0501618690051302 2213 52-53 -0.05702686120194045 2213 54-55 0.04962513029273197 2213 56-57 0.03756970383971492 2213 58-59 0.1759965327276376 2213 60-61 0.2049496320209414 2213 62-63 0.17360701142175117 2213 64-65 0.42128796316370654 2213 66-67 0.22916504905246882 2213 68-69 0.3675396951654335 2213 70-71 -0.19294586851158968 2213 72-73 -0.40266743127212123 2213 74-75 -0.268895686758988 2213 76-77 0.02283608054448294 2213 78-79 -0.21780449752602138 2213 80-81 -0.23045427615778635 2213 82-83 -0.2501311926997367 2213 84-85 -0.2294294931850267 2213 86-87 -0.14971657516445092 2213 88-89 -0.1689084795236937 2213 90-91 -0.22881637318636194 2213 92-93 -0.18432866964272776 2213 94-95 -0.17709751461181966 2213 96-97 -0.19890705708477796 2213 98-99 -0.18403832845522317 2213 100-101 -0.01881149562709794 2212 1 -4.127057995712967 2212 2 -1.9185112603641628 2212 3 -2.0861327748955603 2212 4 -2.75511645883439 2212 5 0.3905854532374313 2212 6 0.030721099179466194 2212 7 -0.09398158497786824 2212 8 -0.09314062146542312 2212 9 -0.06971915390126782 2212 10-11 0.04665372672289436 2212 12-13 -0.15950216353086688 2212 14-15 -0.30329997502020944 2212 16-17 -0.1778414811829876 2212 18-19 -0.11431705680679016 2212 20-21 -0.11989611065009598 2212 22-23 -0.1837030836974023 2212 24-25 -0.1032954258422123 2212 26-27 -0.04916800585693437 2212 28-29 -0.04654589985570112 2212 30-31 -0.10457893023929188 2212 32-33 -0.010631599894786348 2212 34-35 -0.45381832672596545 2212 36-37 -0.1971119630219178 2212 38-39 -0.2508066549143706 2212 40-41 -0.19151225943373618 2212 42-43 -0.10124194084859539 2212 44-45 -0.14621689405824867 2212 46-47 -0.18893372486984106 2212 48-49 -0.16031873301949418 2212 50-51 -0.02088472480681247 2212 52-53 -0.10000790838142137 2212 54-55 -0.12114607449187531 2212 56-57 -0.08932861867255326 2212 58-59 -0.007267346638009542 2212 60-61 0.10708356485690729 2212 62-63 0.05509849293417801 2212 64-65 0.3539892255338657 2212 66-67 0.3550444682239231 2212 68-69 0.4372185023642743 2212 70-71 0.3658407720871857 2212 72-73 -0.4089163530964832 2212 74-75 -0.3772107680252361 2212 76-77 -0.02370327860287702 2212 78-79 -0.30365630030938107 2212 80-81 -0.3265342603178816 2212 82-83 -0.25170411258837433 2212 84-85 -0.3023228053567202 2212 86-87 -0.2963842002497543 2212 88-89 -0.2943857849045024 2212 90-91 -0.27475309572609063 2212 92-93 -0.2400483450084714 2212 94-95 -0.19186953686745412 2212 96-97 -0.24740495001056217 2212 98-99 -0.2129724653742855 2212 100-101 0.04194287220337145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4979.0 3 262.0 4 418.0 5 687.0 6 1327.0 7 3075.0 8 5878.0 9 9678.0 10 13910.0 11 18673.0 12 24928.0 13 32578.0 14 36663.0 15 38093.0 16 42890.0 17 49876.0 18 62555.0 19 81818.0 20 108068.0 21 140524.0 22 182680.0 23 242317.0 24 337823.0 25 514272.0 26 774576.0 27 1091773.0 28 440574.0 29 320006.0 30 385140.0 31 472082.0 32 586512.0 33 742216.0 34 983031.0 35 1371576.0 36 2052663.0 37 2834824.0 38 1821496.0 39 169525.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.352352864004946 21.6488751742945 10.590561543138127 37.408210418562426 2 15.296313884749537 41.153459652687815 19.12408308381596 24.42614337874668 3 10.783499563963373 14.834296080870793 42.06047057952868 32.32173377563715 4 13.775121867156034 16.804831844358077 11.951947833662732 57.468098454823156 5 35.54136268286294 20.362567424669653 14.081437919616087 30.01463197285132 6 34.50080840630053 24.525964032872754 16.5608447850528 24.412382775773924 7 8.10176875 49.67616875 22.063906250000002 20.15815625 8 8.556476036850835 50.464972950249944 18.85670610613287 22.12184490676635 9 27.131978391497295 34.59577307447164 19.65752120719015 18.614727326840917 10-11 17.539963596247723 42.20234013764626 21.45210446575653 18.80559180034949 12-13 7.451534607860457 44.94567015455219 19.130738097835565 28.472057139751783 14-15 8.30207498827812 41.46213117329919 19.29473848552693 30.94105535289576 16-17 17.74413770202582 33.24557185854473 19.381394259011422 29.628896180418035 18-19 10.915116779252939 28.696000816252422 29.327874567127623 31.061007837367015 20-21 22.367482071546814 28.654125072359438 29.304378766331418 19.67401408976233 22-23 7.197487882415176 50.301102107714755 19.994024333170334 22.507385676699737 24-25 7.347425 31.167187499999997 29.277090625000003 32.208296874999995 26-27 7.943718998241218 30.689191584037236 22.365263198914477 39.00182621880707 28-29 17.264907323497894 40.48672214461945 19.10698106513532 23.141389466747338 30-31 7.4169367651563 28.09637838699162 31.64065953396444 32.84602531388764 32-33 8.03274564546603 40.90119118824449 20.248165780103612 30.817897386185866 34-35 7.684411709016052 40.648675111720515 20.87333147352522 30.79358170573821 36-37 17.270767923748405 40.43701490761192 20.418853010477108 21.873364158162566 38-39 7.5589680262590955 30.40931960878358 30.740470809206684 31.291241555750638 40-41 7.576325191983689 39.701543638292854 30.780577936386834 21.941553233336627 42-43 8.059991536411355 29.97085434086436 30.998676271234483 30.970477851489804 44-45 17.43954406646243 30.438877657059045 21.21399829766212 30.907579978816408 46-47 8.308892592059918 39.32803259423881 30.1966553827666 22.16641943093466 48-49 17.946868371685298 29.946306031025436 30.182234692149272 21.924590905139986 50-51 7.678806433732947 29.79542135959982 21.238426768801794 41.28734543786544 52-53 17.168446323628082 29.79779706004249 31.576692544991225 21.4570640713382 54-55 7.481086610372377 29.943681646090624 31.27554504135443 31.299686702182566 56-57 8.001102757271305 29.774777577439654 30.622448791771724 31.601670873517325 58-59 17.355929370731907 29.91763472927768 31.4944643773001 21.23197152269031 60-61 8.072684983809424 30.915518281418557 30.539822030690395 30.47197470408162 62-63 17.217804087313603 31.663509903623332 30.20201439701451 20.916671612048557 64-65 7.463685927358167 50.9866442613712 20.468776181985902 21.080893629284738 66-67 7.450688015658605 51.22721150189462 20.528331048297087 20.793769434149688 68-69 7.523871322425074 50.95913421603924 20.613810522694678 20.903183938841014 70-71 7.69288078060299 49.76123119943161 20.986191444736484 21.559696575228916 72-73 8.140685650074818 47.54551333255354 21.74538058842605 22.568420428945597 74-75 8.585227093215654 45.413693084194755 22.21841072827524 23.78266909431435 76-77 8.982934509796152 43.2152737077973 22.940190563526 24.861601218880548 78-79 9.3511792462559 42.17778337778968 23.249936906115927 25.221100469838497 80-81 9.7919810898434 40.917416972359305 23.45802238674789 25.832579551049406 82-83 10.008808571555448 40.23100289057524 23.382852732790028 26.377335805079284 84-85 10.479035067346436 39.00857914859266 23.7835466547533 26.72883912930761 86-87 10.807177026345691 38.610716614509364 23.68456069085513 26.897545668289812 88-89 11.181837850108531 38.049487265171216 23.550804044851667 27.217870839868585 90-91 11.527193974891716 37.768842557158514 23.52514797207846 27.178815495871305 92-93 11.869101854547164 37.249430820223566 23.3755161524244 27.50595117280487 94-95 12.163701312055625 37.245714749643746 23.12964409088405 27.460939847416583 96-97 12.602678226976755 36.63749203388554 23.110711717267243 27.64911802187046 98-99 12.933254612300738 36.421392191648984 22.83223279408926 27.813120401961015 100-101 13.617141543030497 35.84797039663251 22.43983593309886 28.09505212723813 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 1.0 2 1.0 3 2.5 4 4.0 5 6.0 6 12.5 7 19.5 8 29.5 9 52.5 10 80.5 11 115.5 12 180.5 13 331.5 14 554.0 15 878.5 16 1475.0 17 2469.0 18 3919.0 19 6127.5 20 9694.5 21 15805.5 22 26652.0 23 45989.0 24 77980.0 25 126612.0 26 193696.5 27 275956.5 28 366239.5 29 456828.0 30 537467.5 31 602085.5 32 655475.5 33 693748.0 34 710285.5 35 711933.0 36 705054.5 37 696582.5 38 693318.0 39 696183.5 40 708020.5 41 723286.5 42 732337.5 43 726920.5 44 701451.0 45 658187.5 46 599040.5 47 527731.5 48 449277.0 49 377017.0 50 322521.0 51 270584.5 52 205440.5 53 146625.5 54 108383.0 55 81435.5 56 62102.0 57 48600.0 58 38506.5 59 30896.0 60 25244.5 61 21227.5 62 18071.0 63 15393.0 64 13165.0 65 11345.5 66 9832.0 67 8501.0 68 7466.0 69 6555.0 70 5643.0 71 4783.0 72 4036.0 73 3330.0 74 2698.0 75 2199.0 76 1741.5 77 1343.0 78 1039.5 79 803.0 80 593.5 81 426.5 82 356.0 83 301.0 84 250.5 85 206.5 86 191.5 87 185.5 88 159.5 89 132.0 90 109.5 91 110.0 92 91.0 93 70.5 94 64.5 95 47.0 96 31.0 97 21.0 98 15.5 99 8.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00311875 2 0.0027875 3 0.0084 4 0.01023125 5 6.6875E-4 6 6.2499999999999995E-6 7 0.0 8 2.3124999999999998E-4 9 1.2499999999999999E-5 10-11 6.2499999999999995E-6 12-13 3.1249999999999997E-6 14-15 3.7500000000000003E-5 16-17 1.8750000000000002E-5 18-19 2.96875E-4 20-21 8.75E-5 22-23 7.0E-4 24-25 0.0 26-27 3.1249999999999997E-6 28-29 4.4062500000000004E-4 30-31 7.906250000000001E-4 32-33 6.25E-5 34-35 8.84375E-4 36-37 1.21875E-4 38-39 3.625E-4 40-41 4.5625E-4 42-43 0.005478125 44-45 0.0051625 46-47 0.00686875 48-49 0.016121875 50-51 0.01644375 52-53 0.00238125 54-55 0.004290625 56-57 6.59375E-4 58-59 0.0010875 60-61 6.65625E-4 62-63 0.001403125 64-65 0.00131875 66-67 0.003865625 68-69 0.0016124999999999998 70-71 0.001984375 72-73 2.84375E-4 74-75 9.34375E-4 76-77 0.00250625 78-79 2.125E-4 80-81 8.28125E-4 82-83 0.001521875 84-85 2.75E-4 86-87 1.8750000000000002E-5 88-89 0.003440625 90-91 1.375E-4 92-93 1.5625E-5 94-95 1.9062499999999998E-4 96-97 0.001240625 98-99 2.53125E-4 100-101 3.96875E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.25122955128997 #Duplication Level Percentage of deduplicated Percentage of total 1 88.78757982684955 54.38348433277843 2 7.178689280575405 8.794070900038173 3 1.5848411983141477 2.91220416120844 4 0.754405402472864 1.8483303392639472 5 0.4626302226631387 1.4168334982852149 6 0.28991950020205604 1.0654755514962833 7 0.19229878047255258 0.8244975721610204 8 0.14163080122206329 0.6940048573748573 9 0.11863349673702486 0.6539802787000547 >10 0.4586598161300956 4.56383408633166 >50 0.018182899180531346 0.7278155897683989 >100 0.01018840846567917 1.3632480911201204 >500 0.0013007362126056723 0.5489621436015741 >1k 7.253692503015417E-4 0.8611207802580194 >5k 1.3725595812879194E-4 0.592429141627598 >10k+ 1.770052937244387E-4 18.749708675986167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 2266119 14.163243750000001 TruSeq Adapter, Index 7 (100% over 50bp) GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 147177 0.91985625 TruSeq Adapter, Index 7 (98% over 50bp) CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT 118676 0.741725 TruSeq Adapter, Index 7 (100% over 50bp) GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 91575 0.57234375 TruSeq Adapter, Index 7 (98% over 50bp) GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 64679 0.40424375 TruSeq Adapter, Index 7 (98% over 50bp) GATCCGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 45394 0.2837125 TruSeq Adapter, Index 7 (98% over 50bp) GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 37067 0.23166875 TruSeq Adapter, Index 7 (98% over 50bp) CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 36705 0.22940625 TruSeq Adapter, Index 7 (98% over 50bp) GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT 19509 0.12193124999999999 TruSeq Adapter, Index 7 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCATCTCGTATGC 18385 0.11490624999999999 TruSeq Adapter, Index 7 (98% over 50bp) ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC 18087 0.11304375 No Hit CGCAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT 16264 0.10165 TruSeq Adapter, Index 7 (98% over 50bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.8125E-4 6.25E-6 2.5E-5 2 0.0012125 6.25E-6 3.125E-5 3 0.00133125 6.25E-6 3.75E-5 4 0.00146875 6.25E-6 5.0E-5 5 0.0018125 6.25E-6 5.0E-5 6 0.00205 6.25E-6 5.0E-5 7 0.00235 6.25E-6 5.0E-5 8 0.00324375 1.25E-5 5.0E-5 9 0.003525 2.5E-5 5.0E-5 10-11 0.005678125 2.5E-5 5.0E-5 12-13 0.009184375 2.8125E-5 5.0E-5 14-15 0.014734375000000001 3.125E-5 5.625E-5 16-17 0.017974999999999998 3.125E-5 5.625E-5 18-19 0.019625 3.125E-5 5.625E-5 20-21 0.021656250000000002 3.75E-5 5.625E-5 22-23 0.030415624999999998 3.75E-5 5.625E-5 24-25 0.0483375 3.75E-5 5.625E-5 26-27 0.0556 4.0625E-5 5.625E-5 28-29 0.05770625 4.375E-5 5.625E-5 30-31 0.059328124999999995 4.375E-5 5.625E-5 32-33 0.061668749999999994 4.375E-5 5.625E-5 34-35 0.06400625 4.375E-5 5.625E-5 36-37 0.066809375 4.375E-5 5.625E-5 38-39 0.07019375 4.375E-5 5.625E-5 40-41 0.074828125 4.375E-5 5.625E-5 42-43 0.0801375 4.375E-5 5.625E-5 44-45 0.0871375 4.375E-5 7.5E-5 46-47 0.096609375 4.9999999999999996E-5 7.5E-5 48-49 0.1093625 5.625E-5 7.5E-5 50-51 0.1253375 5.625E-5 7.812499999999999E-5 52-53 0.14621875 5.625E-5 8.125E-5 54-55 0.1731625 5.625E-5 8.125E-5 56-57 0.20823124999999998 5.625E-5 8.75E-5 58-59 0.254246875 5.625E-5 8.75E-5 60-61 0.31343750000000004 5.625E-5 8.75E-5 62-63 0.388546875 5.625E-5 8.75E-5 64-65 0.48390625 5.625E-5 9.375E-5 66-67 0.6021156249999999 5.625E-5 9.687500000000001E-5 68-69 0.7506312500000001 5.625E-5 1.0E-4 70-71 0.936803125 6.25E-5 1.0E-4 72-73 1.163296875 6.25E-5 1.0625E-4 74-75 1.438609375 6.875E-5 1.0625E-4 76-77 1.765075 6.875E-5 1.0625E-4 78-79 2.154109375 6.875E-5 1.0625E-4 80-81 2.613490625 6.875E-5 1.0625E-4 82-83 3.1552218749999996 6.875E-5 1.0625E-4 84-85 3.790396875 6.875E-5 1.0625E-4 86-87 4.530978125000001 6.875E-5 1.0625E-4 88-89 5.382590625 7.5E-5 1.0625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGGA 19065 0.0 86.492546 1 CCGGAAG 19500 0.0 86.1421 3 ACCGGAA 20215 0.0 83.02763 2 TCCGAAG 7310 0.0 75.2539 3 CGAAGAG 8670 0.0 74.505745 5 ACGGAAG 9935 0.0 72.96666 3 TTGGAAG 14050 0.0 72.72804 3 GATTGGA 13910 0.0 72.6101 1 GAGCACA 413105 0.0 72.092186 9 ATTGGAA 14290 0.0 71.44255 2 AGAGCAC 417420 0.0 71.38813 8 CATCGGA 6060 0.0 71.025856 1 AAGAGCA 424745 0.0 70.24422 7 GAAGAGC 426650 0.0 69.96286 6 TGGAAGA 16045 0.0 69.27577 4 CCGAAGA 9185 0.0 69.24791 4 GGAAGAG 427720 0.0 68.08237 5 CGGAAGA 421000 0.0 66.76693 4 GAACGGA 10690 0.0 66.39475 1 GCGGAAG 6670 0.0 65.52391 3 >>END_MODULE