##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_CAGATC_L001_R1_003.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7780221
Sequences flagged as poor quality	0
Sequence length	101
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.731449145210657	30.0	26.0	31.0	16.0	34.0
2	29.607829649054956	31.0	30.0	33.0	25.0	34.0
3	26.217789828849334	30.0	23.0	31.0	10.0	34.0
4	30.78525892773483	35.0	28.0	35.0	19.0	37.0
5	32.60028474769547	35.0	32.0	37.0	25.0	37.0
6	34.141437884605075	35.0	33.0	37.0	30.0	37.0
7	34.83095248836762	35.0	35.0	37.0	32.0	37.0
8	35.042748400077585	35.0	35.0	37.0	32.0	37.0
9	36.72819062594751	39.0	35.0	39.0	32.0	39.0
10-11	36.91205751096274	39.0	37.0	39.0	33.0	39.0
12-13	36.938157283706985	39.0	37.0	39.0	33.0	39.0
14-15	38.15127706526589	40.0	37.0	41.0	33.0	41.0
16-17	38.05840219705841	40.0	37.0	41.0	32.5	41.0
18-19	38.12128453677601	40.0	37.0	41.0	33.0	41.0
20-21	38.106652433137825	40.0	37.0	41.0	33.0	41.0
22-23	38.18568964814753	40.0	37.0	41.0	33.0	41.0
24-25	38.16759151186065	40.0	37.0	41.0	33.0	41.0
26-27	37.951257168658834	40.0	37.0	41.0	33.0	41.0
28-29	37.79316172381222	40.0	37.0	41.0	32.5	41.0
30-31	37.69701329820837	40.0	37.0	41.0	32.5	41.0
32-33	37.652912430122484	40.0	37.0	41.0	32.0	41.0
34-35	36.812020956731175	39.0	36.0	41.0	30.0	41.0
36-37	36.75038922416214	39.5	36.0	41.0	30.0	41.0
38-39	36.68566162837791	39.5	35.5	41.0	30.0	41.0
40-41	37.22779712812785	40.0	36.5	41.0	30.5	41.0
42-43	37.21964388929312	40.0	36.0	41.0	31.0	41.0
44-45	37.097310796184324	40.0	36.0	41.0	30.5	41.0
46-47	36.815926372785555	39.0	36.0	40.5	30.0	41.0
48-49	36.84609177554211	39.0	36.0	41.0	30.0	41.0
50-51	36.77551106838739	39.0	36.0	41.0	30.0	41.0
52-53	36.35204057828177	39.0	35.0	40.5	29.5	41.0
54-55	36.30513619086142	39.0	35.0	40.0	29.0	41.0
56-57	36.175581259195596	39.0	35.0	40.0	29.0	41.0
58-59	35.8141903038487	38.0	34.5	40.0	28.5	41.0
60-61	35.57972524173799	38.0	34.0	40.0	28.0	41.0
62-63	35.11422824621563	37.0	34.0	39.5	28.0	41.0
64-65	34.48371678645118	36.0	33.5	39.0	27.5	40.5
66-67	34.37641064437629	36.0	34.0	39.0	28.0	40.0
68-69	33.141056211642315	35.5	33.0	37.5	25.0	40.0
70-71	29.545841242813026	35.0	29.0	37.0	2.0	39.5
72-73	27.693753429369167	35.0	25.0	36.5	2.0	39.0
74-75	27.27029065112675	34.0	23.5	36.0	2.0	39.0
76-77	25.69440932333413	32.0	21.0	34.5	2.0	36.5
78-79	26.798021932795997	34.0	24.0	35.0	2.0	37.0
80-81	26.80419759284473	34.0	24.0	35.0	2.0	37.0
82-83	26.642001557539302	34.0	24.0	35.0	2.0	36.0
84-85	26.464905752677204	34.0	24.0	35.0	2.0	36.0
86-87	26.296022567996463	34.0	23.0	35.0	2.0	36.0
88-89	26.14910919625548	34.0	22.5	35.0	2.0	36.0
90-91	26.009209892623872	34.0	20.0	35.0	2.0	35.0
92-93	25.88025597216326	34.0	19.5	35.0	2.0	35.0
94-95	25.735371977222755	34.0	18.5	35.0	2.0	35.0
96-97	25.541558588117226	34.0	12.0	35.0	2.0	35.0
98-99	25.31601197446705	34.0	2.0	35.0	2.0	35.0
100-101	24.40668266364156	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2302	1	-5.215375784111863
2302	2	-2.3159064827237614
2302	3	-3.931112138810594
2302	4	-3.6966896854700195
2302	5	-0.7323372430531805
2302	6	-0.7487996431031334
2302	7	-0.48493549303277916
2302	8	-0.36883196130295914
2302	9	-0.43459322118231114
2302	10-11	-0.48928090706111504
2302	12-13	-0.46649316993435264
2302	14-15	-0.6812556701961654
2302	16-17	-0.4690879376879167
2302	18-19	-0.2676669768536115
2302	20-21	-0.3328587360995101
2302	22-23	-0.38017557696925763
2302	24-25	-0.27743473370559
2302	26-27	-0.33857220135843846
2302	28-29	-0.4092842276635267
2302	30-31	-0.43295691144180637
2302	32-33	-0.756770448721312
2302	34-35	-0.7063933217759129
2302	36-37	-0.7067683173858654
2302	38-39	-0.7790427993356914
2302	40-41	-0.4281048032178276
2302	42-43	-0.294018718283958
2302	44-45	-0.3363881068350665
2302	46-47	-0.2899534413764755
2302	48-49	-0.4610642636755671
2302	50-51	-0.295030645995503
2302	52-53	-0.3002793190625894
2302	54-55	-0.340022452188812
2302	56-57	-0.33366829042090274
2302	58-59	-0.2673225651017219
2302	60-61	-0.08513746854777793
2302	62-63	-0.24444899251191288
2302	64-65	0.029208619537982372
2302	66-67	-0.01228386521922431
2302	68-69	0.05387923831764141
2302	70-71	0.0863017551012959
2302	72-73	-0.35200263601957005
2302	74-75	-0.3548290502038185
2302	76-77	-0.2201348331616515
2302	78-79	-0.2771943111476851
2302	80-81	-0.3351984286357812
2302	82-83	-0.30898279408135565
2302	84-85	-0.29260811930851816
2302	86-87	-0.311783227816651
2302	88-89	-0.36492495750641396
2302	90-91	-0.410806603642321
2302	92-93	-0.3530131134020067
2302	94-95	-0.2925831711815583
2302	96-97	-0.4607161615604731
2302	98-99	-0.46270794171471863
2302	100-101	-0.2574972810135776
2306	1	-3.10167805463486
2306	2	-1.4373465088179707
2306	3	-4.134562091854473
2306	4	0.4339359251117223
2306	5	0.2041837234706776
2306	6	-0.005739071792476125
2306	7	-0.11002153118915459
2306	8	-0.08863401827254336
2306	9	-0.20943557134005886
2306	10-11	-0.3236309981697616
2306	12-13	-0.3173444358270956
2306	14-15	-0.3359892667144564
2306	16-17	-0.33654910957550044
2306	18-19	-0.34569603101223123
2306	20-21	-0.2826253492897095
2306	22-23	-0.4157313397234219
2306	24-25	-0.40966732974295184
2306	26-27	-0.3930507673560655
2306	28-29	-0.4642824029272816
2306	30-31	-0.3988839271185114
2306	32-33	-0.5079266169866656
2306	34-35	-0.6719140733045066
2306	36-37	-0.739763313539342
2306	38-39	-0.7327385390651813
2306	40-41	-0.5396396675353117
2306	42-43	-0.4027441456230818
2306	44-45	-0.3362262355880645
2306	46-47	-0.3632609784823799
2306	48-49	-0.3077933238258481
2306	50-51	-0.34629956661996175
2306	52-53	-0.34913808191384277
2306	54-55	-0.3178266104086589
2306	56-57	-0.16342232110146426
2306	58-59	-0.24818785855423187
2306	60-61	-0.23988085359852818
2306	62-63	-0.20349356445826317
2306	64-65	0.14712563905641218
2306	66-67	0.013643554174379346
2306	68-69	0.10288266638666954
2306	70-71	0.3523339774762455
2306	72-73	0.0808791111389553
2306	74-75	0.08458558914723469
2306	76-77	0.02041814362558725
2306	78-79	0.12187773277158698
2306	80-81	0.17885405904524632
2306	82-83	0.22762929711124258
2306	84-85	0.2082715990124413
2306	86-87	0.1803588234944833
2306	88-89	0.19587789760504037
2306	90-91	0.21898188715821476
2306	92-93	0.22444620331036802
2306	94-95	0.254720961245269
2306	96-97	0.24845191250811993
2306	98-99	0.2794358237911325
2306	100-101	0.45130885766189266
2303	1	-3.40487210357135
2303	2	-1.5920949045646289
2303	3	-0.7010553139476592
2303	4	0.28963593049595815
2303	5	-5.220509092264635
2303	6	-2.3538142209232404
2303	7	-0.9051797169810172
2303	8	-0.7646939696841102
2303	9	-0.6319535610911728
2303	10-11	-0.4612559235421969
2303	12-13	-0.502520152741539
2303	14-15	-0.6437118756726932
2303	16-17	-0.6765825842857396
2303	18-19	-0.6608083992960374
2303	20-21	-1.3867665569547327
2303	22-23	-0.6426153058452684
2303	24-25	-0.7556642653514984
2303	26-27	-0.7380126090656205
2303	28-29	-0.7481461054382024
2303	30-31	-0.9169724082847637
2303	32-33	-0.5306119582765092
2303	34-35	-0.7928576123125382
2303	36-37	-0.8773027779360518
2303	38-39	-0.9023979864102074
2303	40-41	-0.8221162845239363
2303	42-43	-0.7479141234623157
2303	44-45	-0.8443500074882664
2303	46-47	-0.7326435757625873
2303	48-49	-0.9666593475452814
2303	50-51	-0.7365658775837076
2303	52-53	-0.6490433052867388
2303	54-55	-0.7748795336543353
2303	56-57	-0.7373491116161119
2303	58-59	-0.6697172798334847
2303	60-61	-0.4135538146254234
2303	62-63	-0.5659502126701668
2303	64-65	-0.6653816090794749
2303	66-67	-0.4438819270597989
2303	68-69	-0.21858335475290147
2303	70-71	0.7597092922591955
2303	72-73	-0.021084769932230074
2303	74-75	-0.12950264301464642
2303	76-77	-0.019708095777581036
2303	78-79	-0.1374573401583845
2303	80-81	-0.05514523770231605
2303	82-83	-0.11221106713952977
2303	84-85	-0.17015583978965054
2303	86-87	-0.288350489044344
2303	88-89	-0.1600430375208255
2303	90-91	-0.11864707969245814
2303	92-93	-0.15189989180498742
2303	94-95	-0.19193445355854166
2303	96-97	-0.5272148224410422
2303	98-99	-0.4318863643074522
2303	100-101	-0.09316023187538747
2307	1	3.8486466335239307
2307	2	2.3811289390842063
2307	3	4.475510960484144
2307	4	3.125001565956204
2307	5	2.7365371269346355
2307	6	1.416562672431688
2307	7	0.7728248570754914
2307	8	0.6097875736136302
2307	9	0.643648429650014
2307	10-11	0.5578984113008545
2307	12-13	0.5752840078248767
2307	14-15	0.5821380437786274
2307	16-17	0.7144670547533991
2307	18-19	0.6012413655532924
2307	20-21	0.7421235447495818
2307	22-23	0.7025695237978127
2307	24-25	0.6904348344814153
2307	26-27	0.6249167725895788
2307	28-29	0.7405039373455367
2307	30-31	0.709193231052879
2307	32-33	0.6958023824459758
2307	34-35	0.9921148907627426
2307	36-37	1.086646424217946
2307	38-39	1.0648666094102168
2307	40-41	0.7879563681752515
2307	42-43	0.6278479877830208
2307	44-45	0.6529611935814685
2307	46-47	0.5288770396129365
2307	48-49	0.6408537190547179
2307	50-51	0.5119315512359677
2307	52-53	0.5372624348573538
2307	54-55	0.393214330611265
2307	56-57	0.2866891688172615
2307	58-59	0.11930044734959466
2307	60-61	-0.0313566582585878
2307	62-63	0.01397080745419288
2307	64-65	-0.7821634949214697
2307	66-67	-0.22320667488582302
2307	68-69	-0.629723203275411
2307	70-71	-0.7595026378057206
2307	72-73	-0.14680273815766753
2307	74-75	-0.05171281609453615
2307	76-77	-0.5322769841416424
2307	78-79	-0.21141177803020383
2307	80-81	-0.18163424466164813
2307	82-83	-0.18638861543668028
2307	84-85	-0.18803369888536992
2307	86-87	-0.18588689760392896
2307	88-89	-0.20264516717928416
2307	90-91	-0.23617042474753092
2307	92-93	-0.2666446684503079
2307	94-95	-0.3001636839370967
2307	96-97	-0.27745802104103845
2307	98-99	-0.2885066947072694
2307	100-101	-0.6534436650404167
2304	1	2.8456537618812625
2304	2	1.7229745397437561
2304	3	4.067136174058351
2304	4	1.1721453243085875
2304	5	1.8871930026652208
2304	6	1.0782026280986088
2304	7	0.6119512008839934
2304	8	0.4721743529648563
2304	9	0.5202429843944856
2304	10-11	0.5303569939228936
2304	12-13	0.46398383414145883
2304	14-15	0.5333893476517559
2304	16-17	0.6110751079903309
2304	18-19	0.5071443548254635
2304	20-21	0.6783777215277667
2304	22-23	0.6037781578525951
2304	24-25	0.5217280038564027
2304	26-27	0.4640450866533641
2304	28-29	0.5441668624198144
2304	30-31	0.603744871459142
2304	32-33	0.6395929463782224
2304	34-35	0.9058579111722551
2304	36-37	0.8950372078243731
2304	38-39	0.8532573033888795
2304	40-41	0.6887408875760457
2304	42-43	0.47952435643630054
2304	44-45	0.5811166153246674
2304	46-47	0.5030884667693698
2304	48-49	0.5351638141193291
2304	50-51	0.45383624459648075
2304	52-53	0.4891880749095705
2304	54-55	0.40636527255154675
2304	56-57	0.35550638788645017
2304	58-59	0.15768613282321553
2304	60-61	0.04751542963842326
2304	62-63	0.13333262112200117
2304	64-65	-0.3724337200123955
2304	66-67	-0.12817426765644058
2304	68-69	-0.6135750121118733
2304	70-71	-0.5292546945491203
2304	72-73	0.09695428416756258
2304	74-75	0.16953674296884813
2304	76-77	-0.2558214855644714
2304	78-79	0.046440981686284744
2304	80-81	0.07016311280418464
2304	82-83	0.015300804288191472
2304	84-85	-0.0015353947319098893
2304	86-87	3.578975760056835E-4
2304	88-89	0.002705366815842325
2304	90-91	-0.024484398133743923
2304	92-93	-0.04735721621412026
2304	94-95	-0.045741731023515086
2304	96-97	-0.050036809878019284
2304	98-99	-0.09563112977934196
2304	100-101	-0.4333194915103107
2301	1	2.6485602210432155
2301	2	1.7859008597821813
2301	3	3.297004903401522
2301	4	-0.8480907256439707
2301	5	1.0656739274653688
2301	6	0.6192251425987081
2301	7	0.4110236918090493
2301	8	0.2905015373841593
2301	9	0.42646128130160577
2301	10-11	0.4415645585133845
2301	12-13	0.4337918987546914
2301	14-15	0.46768350008893833
2301	16-17	0.578028908795531
2301	18-19	0.48597247336337546
2301	20-21	0.6402854953342754
2301	22-23	0.5648087634748507
2301	24-25	0.5099922258516756
2301	26-27	0.4786127432546863
2301	28-29	0.5945878857361251
2301	30-31	0.5872419366719939
2301	32-33	0.6136797191474557
2301	34-35	0.8347520408151112
2301	36-37	0.8755662258774635
2301	38-39	0.8785156644451391
2301	40-41	0.6674655242346788
2301	42-43	0.5251090189325609
2301	44-45	0.5353568009000966
2301	46-47	0.30671723511119353
2301	48-49	0.45733465811541407
2301	50-51	0.3929710273660092
2301	52-53	0.44461372256350984
2301	54-55	0.3618413219678658
2301	56-57	0.3270188358576078
2301	58-59	0.22698935875260418
2301	60-61	0.12405825775174861
2301	62-63	0.22839958614301992
2301	64-65	-0.3991058728702228
2301	66-67	-0.07078765254630781
2301	68-69	-0.4317847269029258
2301	70-71	-0.5945824397099742
2301	72-73	-0.04115664237648531
2301	74-75	0.06406516054391176
2301	76-77	-0.211633761904352
2301	78-79	-0.03734988287961727
2301	80-81	-0.00878463984615152
2301	82-83	-0.019260250707159088
2301	84-85	-0.020949313005033332
2301	86-87	-0.01926158015986701
2301	88-89	-0.060725401195096396
2301	90-91	-0.06983171481817863
2301	92-93	-0.09052601284033202
2301	94-95	-0.060646231759054814
2301	96-97	-0.0374334332855355
2301	98-99	-0.04705967164166225
2301	100-101	-0.3315997953823704
2305	1	2.743814997535484
2305	2	1.5231890721631913
2305	3	3.0355492783723115
2305	4	2.67252096447303
2305	5	1.1002562498396813
2305	6	0.626800087099177
2305	7	0.3746383670756046
2305	8	0.3102224724617173
2305	9	0.18640619053091
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2313	36-37	-0.1867705334745864
2313	38-39	-0.23845077184626007
2313	40-41	-0.08853648422440585
2313	42-43	-0.0481386601963365
2313	44-45	0.045636123706884746
2313	46-47	0.05602045083701057
2313	48-49	-0.005969814146446595
2313	50-51	0.03125444495260865
2313	52-53	0.062220785268856105
2313	54-55	0.0037465994225485133
2313	56-57	-0.07384453359567544
2313	58-59	0.04663866459604549
2313	60-61	0.08510326686506886
2313	62-63	0.10019495138747203
2313	64-65	0.2502881206929146
2313	66-67	0.15901180973754947
2313	68-69	0.22955003278718067
2313	70-71	0.11007439093663152
2313	72-73	-0.4254441367525672
2313	74-75	-0.43787305954138844
2313	76-77	-0.3138678359779874
2313	78-79	-0.2670525108834596
2313	80-81	-0.2699269295704916
2313	82-83	-0.2626597248038074
2313	84-85	-0.2245207252824457
2313	86-87	-0.2120524975380853
2313	88-89	-0.23283772061215302
2313	90-91	-0.18958598689968653
2313	92-93	-0.21021646872726762
2313	94-95	-0.200220886735341
2313	96-97	-0.14690528388564061
2313	98-99	-0.12758468293849745
2313	100-101	-0.05251174601119146
2316	1	-0.6010318394771481
2316	2	0.03192179312945598
2316	3	0.22002592420638933
2316	4	-0.12565001149796373
2316	5	0.8330569678557822
2316	6	0.2736114056003913
2316	7	0.05731034557942394
2316	8	-0.03633348965815486
2316	9	-0.12188499884867099
2316	10-11	-0.13160292605843438
2316	12-13	-0.12474777450893271
2316	14-15	-0.2015013279700355
2316	16-17	-0.09551593235865852
2316	18-19	-0.06161992590853771
2316	20-21	-0.1464450619644495
2316	22-23	-0.17401673622951108
2316	24-25	-0.1751330736460588
2316	26-27	-0.16071535087106525
2316	28-29	-0.1582513462479369
2316	30-31	-0.16554607769026575
2316	32-33	-0.23586963492942914
2316	34-35	-0.38657786139771844
2316	36-37	-0.4059823290144635
2316	38-39	-0.4550768117982571
2316	40-41	-0.25587222279222743
2316	42-43	-0.3108095938855726
2316	44-45	-0.17251691861215335
2316	46-47	-0.22033357392254516
2316	48-49	-0.21002738680135735
2316	50-51	-0.1820395078212087
2316	52-53	-0.2491453774580492
2316	54-55	-0.18969993793916018
2316	56-57	-0.16473702918592892
2316	58-59	-0.04077302590405907
2316	60-61	-0.054216159898700766
2316	62-63	-0.08299592181695914
2316	64-65	0.1350121929562107
2316	66-67	0.037819727887487886
2316	68-69	0.05419155538181286
2316	70-71	0.24896215788541554
2316	72-73	-0.24911878781233554
2316	74-75	-0.3191037100797942
2316	76-77	-0.2520470501492724
2316	78-79	-0.290034231825004
2316	80-81	-0.28345320262518925
2316	82-83	-0.2724514762972561
2316	84-85	-0.24286763541824996
2316	86-87	-0.27829837571215066
2316	88-89	-0.2469076108306858
2316	90-91	-0.23922916077994216
2316	92-93	-0.2321606935644951
2316	94-95	-0.23254440886612215
2316	96-97	-0.18478228101171013
2316	98-99	-0.15737577197335284
2316	100-101	-0.09599415174363202
2216	1	-4.792387627261537
2216	2	-2.3571078146764712
2216	3	-4.353826661539088
2216	4	-3.4144972886437692
2216	5	-0.8426619335391443
2216	6	-0.6630750713660944
2216	7	-0.4650005105734536
2216	8	-0.3654849021509463
2216	9	-0.39852223821779376
2216	10-11	-0.4239066461795957
2216	12-13	-0.3256761639318171
2216	14-15	-0.33634461439709895
2216	16-17	-0.35583374333281625
2216	18-19	-0.40574046101236405
2216	20-21	-0.4582421521636846
2216	22-23	-0.5845732970739945
2216	24-25	-0.4820779929379668
2216	26-27	-0.49972043790447884
2216	28-29	-0.47745796569959964
2216	30-31	-0.47384211476492055
2216	32-33	-0.5172570035141533
2216	34-35	-0.8084835924054445
2216	36-37	-0.8310230972820705
2216	38-39	-0.7942201544581451
2216	40-41	-0.7271987138913403
2216	42-43	-0.5483259252657433
2216	44-45	-0.586659065121701
2216	46-47	-0.45739019164712147
2216	48-49	-0.5487595995177799
2216	50-51	-0.5126134866648115
2216	52-53	-0.5146718652397198
2216	54-55	-0.5058056817026255
2216	56-57	-0.43428616296142764
2216	58-59	-0.487660646191479
2216	60-61	-0.42679152273706933
2216	62-63	-0.36829253556545893
2216	64-65	-0.059217377466737275
2216	66-67	-0.11071175413380985
2216	68-69	0.09907969908574898
2216	70-71	0.7183350909980533
2216	72-73	-0.022215798714643853
2216	74-75	-0.21625104229185865
2216	76-77	0.039360588701139676
2216	78-79	-0.1612913715521671
2216	80-81	-0.21494939444393069
2216	82-83	-0.19283599636430537
2216	84-85	-0.2091937346123096
2216	86-87	-0.14472396672448795
2216	88-89	-0.10609658386211507
2216	90-91	-0.08623569868771597
2216	92-93	-0.09070698557001933
2216	94-95	-0.07906205448497516
2216	96-97	-0.05407681956717525
2216	98-99	-0.05231803278361724
2216	100-101	0.04001397644704241
2215	1	-2.8431093819291284
2215	2	-1.2452438356558062
2215	3	-2.4736609842321045
2215	4	-1.0035983421291874
2215	5	0.4472571412383317
2215	6	-0.10163330387529612
2215	7	-0.16966005993134559
2215	8	-0.36347897262075435
2215	9	-0.452642581157221
2215	10-11	-0.34755524894902123
2215	12-13	-0.547986375803589
2215	14-15	-0.5717262785454267
2215	16-17	-0.4683380832636601
2215	18-19	-0.48055858472978485
2215	20-21	-0.6275336148249409
2215	22-23	-0.5520531673045568
2215	24-25	-0.5613858477324669
2215	26-27	-0.4762423998594798
2215	28-29	-0.5917337156651854
2215	30-31	-0.5002891912509
2215	32-33	-0.47820002955918284
2215	34-35	-0.7766163150123759
2215	36-37	-0.8057713749847935
2215	38-39	-0.7717509211340996
2215	40-41	-0.5829787535811093
2215	42-43	-0.43205777176925864
2215	44-45	-0.49784203897149126
2215	46-47	-0.4633586920853574
2215	48-49	-0.49413438769060747
2215	50-51	-0.47833865764212646
2215	52-53	-0.3818352975730974
2215	54-55	-0.2710408922042049
2215	56-57	-0.3059256920373201
2215	58-59	-0.2788655460654539
2215	60-61	-0.23193114998198894
2215	62-63	-0.27494785661099996
2215	64-65	0.09369410223435892
2215	66-67	-0.10331577874593023
2215	68-69	0.13402183350476804
2215	70-71	1.5470825934844186
2215	72-73	0.3255302400560218
2215	74-75	-0.03772516677489435
2215	76-77	0.06205342461821317
2215	78-79	0.010614656598811223
2215	80-81	0.030380496692398395
2215	82-83	0.046453845892276746
2215	84-85	0.0461355193975308
2215	86-87	0.05530656944559098
2215	88-89	0.002159231926345484
2215	90-91	-0.0035537769633009475
2215	92-93	0.04323900942431891
2215	94-95	0.0632235329645674
2215	96-97	0.06592826609007929
2215	98-99	0.07423000744175567
2215	100-101	0.2976349204120865
2214	1	-3.825234731810692
2214	2	-5.607286223554205
2214	3	-0.1807146991176296
2214	4	1.9900004096586912
2214	5	-4.204112336951319
2214	6	-1.8828357114017678
2214	7	-1.298592990437875
2214	8	-1.008136669228925
2214	9	-1.2348093741586936
2214	10-11	-1.052516397881746
2214	12-13	-0.9052099667327198
2214	14-15	-0.8339494677471464
2214	16-17	-1.0032376557259894
2214	18-19	-0.8563161812608513
2214	20-21	-1.372983826609108
2214	22-23	-1.0860615544907688
2214	24-25	-0.9548009578412504
2214	26-27	-1.0050837384343865
2214	28-29	-1.3048081043635875
2214	30-31	-1.3038843807042042
2214	32-33	-1.0978390683740997
2214	34-35	-1.2703102205870707
2214	36-37	-1.505119303806751
2214	38-39	-1.3159474869304404
2214	40-41	-1.336557552747891
2214	42-43	-1.1880125368795333
2214	44-45	-1.2607846797759876
2214	46-47	-1.2337726165709597
2214	48-49	-1.1743973978580513
2214	50-51	-1.0957575317162096
2214	52-53	-1.3079542210625377
2214	54-55	-1.1205221365198597
2214	56-57	-1.146631761994655
2214	58-59	-1.0620195050746872
2214	60-61	-0.8605536789509429
2214	62-63	-1.1926656412847265
2214	64-65	-0.5850090119104934
2214	66-67	-0.7044691414919768
2214	68-69	-0.08301300978031634
2214	70-71	1.8300318920338476
2214	72-73	1.0030461945785625
2214	74-75	0.6617969837457522
2214	76-77	0.7769382736271915
2214	78-79	0.6011787988513504
2214	80-81	0.5350301103341799
2214	82-83	0.5590464061883047
2214	84-85	0.5624604628920338
2214	86-87	0.544131284751252
2214	88-89	0.48322771034207435
2214	90-91	0.4367400957445291
2214	92-93	0.4884545510401175
2214	94-95	0.3538016613712216
2214	96-97	0.4787781260799271
2214	98-99	0.4832163619409755
2214	100-101	0.5781067880840354
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2700.0
3	129.0
4	188.0
5	327.0
6	585.0
7	1422.0
8	2690.0
9	4482.0
10	6299.0
11	8546.0
12	11490.0
13	14889.0
14	16490.0
15	17254.0
16	18889.0
17	22101.0
18	27658.0
19	35871.0
20	48050.0
21	62285.0
22	82015.0
23	108193.0
24	151583.0
25	232044.0
26	361044.0
27	536926.0
28	264274.0
29	157650.0
30	187592.0
31	229187.0
32	281861.0
33	352796.0
34	465213.0
35	648656.0
36	979880.0
37	1397828.0
38	946989.0
39	94125.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.518748786174882	22.21636401424804	11.125906585175596	37.13898061440148
2	15.214697447270186	40.96970237581876	18.9799311866909	24.83566899022015
3	11.107786089902318	14.708578387610663	42.22765794632722	31.955977576159807
4	13.887789494981643	16.9176476909493	11.772921248932617	57.42164156513644
5	35.37405208092337	19.92717410863474	14.960695097812396	29.738078712629495
6	34.457890945173105	24.59135126728503	16.572164136852262	24.378593650689602
7	8.089516921469293	49.67883237009822	22.07482964780491	20.156821060627582
8	8.5841263377994	50.39615198591403	18.842498175823025	22.177223500463548
9	27.061814825054455	34.6587069955982	19.628285623249006	18.65119255609834
10-11	17.479157563722005	42.38973047100657	21.500971598427064	18.63014036684436
12-13	7.424570587390769	45.031272247922004	19.105819744709052	28.438337419978172
14-15	8.28018895607207	41.493294342153	19.288173176571718	30.938343525203205
16-17	17.717517683951787	33.29553876253853	19.382702387002524	29.604241166507162
18-19	10.940722625893223	28.723041564063728	29.278406857309285	31.05782895273376
20-21	22.369643112827944	28.705937288646666	29.253126326280317	19.671293272245073
22-23	7.15323605475901	50.315506068675106	19.980221585591835	22.55103629097405
24-25	7.306920452561252	31.229698080635774	29.25294463585253	32.21043683095044
26-27	7.929900106186059	30.757139442803066	22.375773460657207	38.937186990353666
28-29	17.136048284613725	40.55248701158235	19.13099674417152	23.180467959632406
30-31	7.360588139613751	28.130913453502153	31.640479121759636	32.86801928512446
32-33	7.973067417892995	41.05725230208733	20.225297206004882	30.7443830740148
34-35	7.649174284191329	40.71282601478128	20.88742036126842	30.75057933975897
36-37	16.99672738071758	40.64857539231683	20.478699697609027	21.875997529356564
38-39	7.544018589357622	30.473729267833573	30.714733603161022	31.26751853964779
40-41	7.537952513456303	39.73908454338414	30.770092425846542	21.952870517313023
42-43	8.052717991470566	30.001154884244546	30.989721658758246	30.956405465526643
44-45	17.39099193963965	30.498425577308673	21.21108627003409	30.899496213017585
46-47	8.301631577962922	39.33641865304397	30.16052245564127	22.201427313351836
48-49	17.901951654196687	29.98872383288135	30.15683480656184	21.952489706360126
50-51	7.669883546792418	29.85166147619076	21.24582735427407	41.232627622742754
52-53	17.114213239236527	29.851175697019666	31.562715912266942	21.471895151476865
54-55	7.4649869169881855	29.971803161323514	31.27655041642955	31.28665950525875
56-57	7.991239807693754	29.80739106201008	30.595809654887162	31.605559475409002
58-59	17.32173980103339	29.956459988175112	31.46802781419501	21.25377239659649
60-61	8.064713490018995	30.98627674426914	30.520407378949155	30.428602386762705
62-63	17.17139746284152	31.766427540137226	30.127487734445264	20.934687262575995
64-65	7.428200702882319	51.05435239808705	20.438188032748904	21.079258866281734
66-67	7.429331965761872	51.27816519209302	20.48781428276725	20.80468855937786
68-69	7.44343087646924	51.077527924674584	20.593978367695705	20.88506283116047
70-71	7.624000235469314	50.06046754283015	20.88556898293323	21.429963238767296
72-73	8.003615835591303	48.12365869812695	21.592008761704843	22.2807167045769
74-75	8.48088649438316	45.656359136957406	22.14604898307162	23.716705385587822
76-77	8.881491487786587	43.39013198032702	22.885728940677268	24.842647591209126
78-79	9.222429782568934	42.39206565619497	23.16448021343482	25.221024347801276
80-81	9.63444924589962	41.119831078121805	23.417621825348295	25.82809785063028
82-83	10.032967822567098	39.68599331568657	23.476057645872782	26.804981215873553
84-85	10.399830955784886	39.15546399565154	23.665007628968514	26.779697419595067
86-87	10.844173871913483	38.225574888081425	23.681815786463346	27.24843545354174
88-89	11.060834535318307	37.97992747217097	23.470569983802445	27.488668008708274
90-91	11.47015798919524	37.634942222383316	23.43509823602275	27.459801552398694
92-93	11.830239524594782	37.11555701314407	23.363710686161077	27.690492776100072
94-95	12.234968661522334	36.94182754917218	23.048754969658923	27.774448819646558
96-97	12.510364550698094	36.8773702222008	23.07047765942064	27.54178756768047
98-99	12.93386909419248	36.20686452972846	22.79428743109523	28.06497894498383
100-101	13.5044221614939	35.91779299434712	22.35774236061247	28.22004248354651
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.5
4	3.5
5	3.0
6	5.0
7	10.0
8	14.5
9	27.0
10	40.0
11	59.5
12	94.0
13	153.5
14	256.0
15	428.5
16	744.5
17	1212.5
18	1890.5
19	2946.5
20	4704.0
21	7839.5
22	13474.5
23	22996.5
24	38804.0
25	62711.0
26	95266.5
27	135413.5
28	179834.0
29	224064.0
30	263054.0
31	294859.5
32	320204.5
33	337837.0
34	346043.5
35	345607.0
36	341310.5
37	337639.5
38	336841.5
39	338877.0
40	343954.5
41	350881.0
42	354790.5
43	351968.5
44	339810.0
45	319113.0
46	290121.0
47	254857.0
48	217026.0
49	182528.0
50	156816.5
51	131657.5
52	99577.5
53	71017.0
54	52565.5
55	39591.5
56	30288.5
57	23660.5
58	18689.5
59	15020.5
60	12167.5
61	10088.0
62	8684.0
63	7477.5
64	6389.0
65	5468.0
66	4743.0
67	4140.5
68	3664.0
69	3175.0
70	2721.0
71	2324.0
72	1985.0
73	1679.0
74	1331.0
75	1021.0
76	819.0
77	647.0
78	490.5
79	385.0
80	291.0
81	223.0
82	178.0
83	145.5
84	116.5
85	93.5
86	83.0
87	79.0
88	73.0
89	61.5
90	53.5
91	47.5
92	43.0
93	38.0
94	27.5
95	20.0
96	19.0
97	13.5
98	6.5
99	2.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.225987410897455E-4
2	5.141242131810909E-4
3	0.010565252580871418
4	0.008765817834737599
5	2.699152119200727E-4
6	7.711863197716362E-5
7	2.0564968527243637E-4
8	0.0
9	0.0
10-11	6.4265526647636365E-6
12-13	0.0
14-15	0.0
16-17	3.855931598858181E-5
18-19	3.406072912324727E-4
20-21	3.855931598858181E-5
22-23	2.8276831724959994E-4
24-25	1.8637002727814545E-4
26-27	1.2210450063050908E-4
28-29	2.5706210659054543E-4
30-31	3.53460396562E-4
32-33	6.87641135129709E-4
34-35	0.0031361577004046545
36-37	7.904659777659272E-4
38-39	9.061439257316726E-4
40-41	4.884180025220363E-4
42-43	0.0028341097251607635
44-45	0.006992089299262837
46-47	0.01722958769423131
48-49	0.0069213972199504365
50-51	0.026123936582264178
52-53	8.354518464192726E-5
54-55	0.0012017653483108
56-57	3.406072912324727E-4
58-59	2.699152119200727E-4
60-61	0.0018379940621223998
62-63	0.001285310532952727
64-65	0.0014588274549013453
66-67	7.711863197716362E-5
68-69	6.426552664763636E-5
70-71	1.799434746133818E-4
72-73	0.0
74-75	3.213276332381818E-5
76-77	0.004003742310147745
78-79	0.005424010449060509
80-81	7.262004511182908E-4
82-83	0.006606496139377018
84-85	1.0282484263621818E-4
86-87	1.4138415862479997E-4
88-89	4.4985868653345457E-4
90-91	0.0016966099034976
92-93	0.003181143569058
94-95	1.1567794796574546E-4
96-97	0.001233898111634618
98-99	1.0925139530098182E-4
100-101	4.1772592320963637E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7780221.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.84734917993633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.47730800095225	65.98989521522904
2	3.6300007040533266	5.070918533988549
3	0.8216030183901024	1.7216037873834946
4	0.3750088005428749	1.0477348254826913
5	0.19051225761940183	0.6653388090500173
6	0.1107226301499631	0.4640209326123251
7	0.08877301850979559	0.43403920151274505
8	0.0650734427814672	0.3636165984238199
9	0.03537138617823337	0.22235378052324964
>10	0.18467926967765746	2.189960435053158
>50	0.010385997859375133	0.49402658380064324
>100	0.008713079311819857	1.2430206444300345
>500	7.154127843174077E-4	0.33395884011329274
>1k	8.348841351991524E-4	1.1256708703568137
>5k	1.6769800661485252E-4	0.8948117755740268
>10k+	1.3039904759833126E-4	17.739029166466082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	1120038	14.395966387073067	TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	69546	0.8938820632473036	TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT	60630	0.7792837761292385	TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	44080	0.5665648829255622	TruSeq Adapter, Index 7 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	29196	0.37525926320087827	TruSeq Adapter, Index 7 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	24144	0.31032537507610647	TruSeq Adapter, Index 7 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	15704	0.20184516609489628	TruSeq Adapter, Index 7 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT	9782	0.12572907633343577	TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCATCTCGTATGC	9477	0.12180887920792995	TruSeq Adapter, Index 7 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT	9428	0.12117907704678312	TruSeq Adapter, Index 7 (98% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	8983	0.11545944517514348	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	9.511297943850182E-4	0.0	0.0
2	0.0013495760596003636	0.0	0.0
3	0.0015295195342137453	0.0	0.0
4	0.0016194912715204362	0.0	0.0
5	0.0019279657994290909	0.0	0.0
6	0.0023521182753034908	0.0	0.0
7	0.002712005224530254	0.0	0.0
8	0.003598869492267636	0.0	0.0
9	0.0038045191775400726	0.0	1.2853105329527271E-5
10-11	0.006143784347514036	0.0	1.9279657994290906E-5
12-13	0.009594843128492108	6.426552664763636E-6	2.5706210659054542E-5
14-15	0.015153811183512653	1.2853105329527271E-5	2.5706210659054542E-5
16-17	0.018566310648502143	1.2853105329527271E-5	2.5706210659054542E-5
18-19	0.020192228472687344	1.2853105329527271E-5	2.5706210659054542E-5
20-21	0.021715321454236325	2.5706210659054542E-5	2.5706210659054542E-5
22-23	0.030706068632240652	2.5706210659054542E-5	2.5706210659054542E-5
24-25	0.04970295830928196	2.5706210659054542E-5	2.5706210659054542E-5
26-27	0.05660507587123811	2.5706210659054542E-5	2.5706210659054542E-5
28-29	0.05882223654058156	2.5706210659054542E-5	2.5706210659054542E-5
30-31	0.060634524392044906	2.5706210659054542E-5	2.5706210659054542E-5
32-33	0.06302520198333697	2.5706210659054542E-5	2.5706210659054542E-5
34-35	0.06521022988935662	2.5706210659054542E-5	2.5706210659054542E-5
36-37	0.06771015887594967	2.5706210659054542E-5	2.5706210659054542E-5
38-39	0.07127046905222872	2.5706210659054542E-5	2.5706210659054542E-5
40-41	0.07580118868088709	2.5706210659054542E-5	2.5706210659054542E-5
42-43	0.08126375844593617	2.5706210659054542E-5	3.855931598858182E-5
44-45	0.08853861606244862	3.213276332381818E-5	5.1412421318109085E-5
46-47	0.09847406648217319	3.855931598858182E-5	5.1412421318109085E-5
48-49	0.11142999665433669	3.855931598858182E-5	5.1412421318109085E-5
50-51	0.12772773421217726	5.1412421318109085E-5	5.1412421318109085E-5
52-53	0.14885823937392012	5.1412421318109085E-5	5.1412421318109085E-5
54-55	0.17653097514839233	5.1412421318109085E-5	5.1412421318109085E-5
56-57	0.21140787646006456	5.1412421318109085E-5	5.1412421318109085E-5
58-59	0.2574605528557608	5.1412421318109085E-5	5.1412421318109085E-5
60-61	0.31632777526499567	5.1412421318109085E-5	6.426552664763636E-5
62-63	0.39206469841923514	5.1412421318109085E-5	6.426552664763636E-5
64-65	0.4888035956819221	5.1412421318109085E-5	6.426552664763636E-5
66-67	0.6089608508550078	5.1412421318109085E-5	6.426552664763636E-5
68-69	0.7569515056191849	5.1412421318109085E-5	7.711863197716364E-5
70-71	0.9426724510781892	5.1412421318109085E-5	7.711863197716364E-5
72-73	1.1690349155891586	5.1412421318109085E-5	7.711863197716364E-5
74-75	1.4426711015021296	5.1412421318109085E-5	7.711863197716364E-5
76-77	1.7722375752565385	5.1412421318109085E-5	7.711863197716364E-5
78-79	2.169404956491596	5.1412421318109085E-5	7.711863197716364E-5
80-81	2.6332606747289056	5.1412421318109085E-5	7.711863197716364E-5
82-83	3.1766642618506595	5.1412421318109085E-5	7.711863197716364E-5
84-85	3.8142104189585364	5.1412421318109085E-5	8.354518464192726E-5
86-87	4.55541687054905	5.1412421318109085E-5	8.99717373066909E-5
88-89	5.415212498462447	5.1412421318109085E-5	8.99717373066909E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGA	8700	0.0	86.49697	1
CCGGAAG	9285	0.0	86.059875	3
ACCGGAA	9605	0.0	83.342155	2
CATCGGA	3700	0.0	74.72849	1
GAGCACA	202050	0.0	71.90654	9
GATTGGA	6775	0.0	71.45455	1
TTGGAAG	6850	0.0	71.43372	3
AGAGCAC	204200	0.0	71.209915	8
ACGGAAG	4805	0.0	70.592964	3
ATTGGAA	6990	0.0	70.207794	2
AAGAGCA	207830	0.0	70.052986	7
GAAGAGC	208115	0.0	70.016396	6
TGGAAGA	7560	0.0	69.67949	4
GGAAGAG	208550	0.0	68.12587	5
GAACGGA	4770	0.0	66.82964	1
CGGAAGA	205025	0.0	66.689064	4
TCGGAAG	189185	0.0	65.31209	3
GCGGAAG	3140	0.0	64.754616	3
TCTGAAG	4105	0.0	64.576965	3
ATCGGAA	193390	0.0	63.529217	2
>>END_MODULE