##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2112_lane1_GCCAAT_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.5612831875 31.0 28.0 31.0 16.0 34.0 2 30.067619625 31.0 30.0 33.0 25.0 34.0 3 27.05495475 31.0 25.0 31.0 16.0 34.0 4 31.67013675 35.0 30.0 37.0 19.0 37.0 5 32.585261125 35.0 32.0 37.0 25.0 37.0 6 34.068889 35.0 33.0 37.0 30.0 37.0 7 34.7346688125 35.0 35.0 37.0 32.0 37.0 8 34.9535398125 35.0 35.0 37.0 32.0 37.0 9 36.55904825 38.0 35.0 39.0 32.0 39.0 10-11 36.6663126875 39.0 36.0 39.0 32.5 39.0 12-13 36.722070125 39.0 36.0 39.0 32.5 39.0 14-15 37.9354190625 40.0 37.0 41.0 33.0 41.0 16-17 37.861194187500004 40.0 37.0 41.0 32.5 41.0 18-19 37.85772240625 39.5 37.0 41.0 33.0 41.0 20-21 37.83964040625 40.0 37.0 41.0 32.5 41.0 22-23 37.975459906249995 40.0 37.0 41.0 33.0 41.0 24-25 37.84426415625 40.0 37.0 41.0 33.0 41.0 26-27 37.47123171875 40.0 37.0 41.0 32.0 41.0 28-29 37.33693915625 39.5 36.5 41.0 31.5 41.0 30-31 37.3150234375 39.0 36.5 41.0 31.0 41.0 32-33 37.1449609375 39.0 36.0 41.0 31.0 41.0 34-35 36.47656825 39.0 35.0 40.0 30.0 41.0 36-37 36.3445406875 39.0 35.0 40.0 30.0 41.0 38-39 36.2968656875 39.0 35.0 40.0 30.0 41.0 40-41 36.85845475 39.0 36.0 40.0 30.0 41.0 42-43 36.803199 39.0 36.0 40.5 30.0 41.0 44-45 36.6359329375 39.0 35.5 40.5 30.0 41.0 46-47 36.51593540625 39.0 35.0 40.0 30.0 41.0 48-49 36.476873 39.0 35.0 40.0 29.5 41.0 50-51 36.32673596875 39.0 35.0 40.0 29.0 41.0 52-53 35.97412034375 38.5 35.0 40.0 28.0 41.0 54-55 35.9163020625 38.5 35.0 40.0 28.0 41.0 56-57 35.759823875 38.0 34.0 40.0 28.0 41.0 58-59 35.36075565625001 38.0 34.0 40.0 28.0 41.0 60-61 35.03367584375 37.5 34.0 40.0 28.0 41.0 62-63 34.71662446875 37.0 33.5 39.5 27.0 41.0 64-65 34.08501065625 36.0 33.0 39.0 26.0 41.0 66-67 33.88632378125 36.0 33.0 39.0 26.0 40.5 68-69 32.8514825 35.5 32.5 39.0 22.5 40.0 70-71 30.18661746875 35.0 30.0 38.0 2.0 40.0 72-73 29.35428603125 35.0 29.0 37.0 2.0 40.0 74-75 28.980204937499998 35.0 29.0 37.0 2.0 39.0 76-77 26.6126621875 32.0 24.5 34.5 2.0 36.5 78-79 28.310675875 34.0 28.5 36.0 2.0 37.5 80-81 28.38278603125 34.0 29.0 35.5 2.0 37.0 82-83 28.2048044375 34.0 29.0 35.0 2.0 37.0 84-85 28.0162853125 34.0 29.0 35.0 2.0 36.5 86-87 27.83166903125 34.0 29.0 35.0 2.0 36.0 88-89 27.61631028125 34.0 29.0 35.0 2.0 36.0 90-91 27.464761000000003 34.0 29.0 35.0 2.0 36.0 92-93 27.285237125 34.0 27.5 35.0 2.0 35.5 94-95 27.094460124999998 34.0 27.0 35.0 2.0 35.0 96-97 26.9056433125 34.0 27.0 35.0 2.0 35.0 98-99 26.65384209375 34.0 26.5 35.0 2.0 35.0 100-101 25.536492375 33.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.493684240783761 1101 2 1.4874841388320768 1101 3 3.016624722895081 1101 4 2.518724590409896 1101 5 2.892223004724137 1101 6 1.4234976505248582 1101 7 0.6967248094296679 1101 8 0.517353280825688 1101 9 0.5870001563714737 1101 10-11 0.5797386074154574 1101 12-13 0.4910499494514866 1101 14-15 0.44585355362511336 1101 16-17 0.46387699639646485 1101 18-19 0.35851401780950454 1101 20-21 0.571840370638995 1101 22-23 0.33650843354995885 1101 24-25 0.4314805856865931 1101 26-27 0.42698457306398296 1101 28-29 0.6440065683299636 1101 30-31 0.6278474198347652 1101 32-33 0.6776272588407366 1101 34-35 0.7687370744868147 1101 36-37 0.8816014223210757 1101 38-39 0.8350917847431347 1101 40-41 0.6070977585842883 1101 42-43 0.44222552541339155 1101 44-45 0.43136936124648884 1101 46-47 0.39901809917190434 1101 48-49 0.4816498187587115 1101 50-51 0.32339911777533814 1101 52-53 0.3081838586041883 1101 54-55 0.17387948598874914 1101 56-57 0.1185587758237645 1101 58-59 0.016990392565027435 1101 60-61 -0.037783736310139204 1101 62-63 -0.009327063975021588 1101 64-65 -0.5128891975120595 1101 66-67 -0.23657654392880545 1101 68-69 -0.5753481907368325 1101 70-71 0.020242572720530205 1101 72-73 0.36069514638603906 1101 74-75 0.39250378801484587 1101 76-77 -0.5022923825360088 1101 78-79 0.16732862202683307 1101 80-81 0.2999100190482622 1101 82-83 0.27423428500291536 1101 84-85 0.25824838650815707 1101 86-87 0.27715623706010106 1101 88-89 0.21486583976117757 1101 90-91 0.18429979641853222 1101 92-93 0.16238181368958493 1101 94-95 0.15108034765462364 1101 96-97 0.1575325466733517 1101 98-99 0.09298700383394376 1101 100-101 -0.35520480432782264 1102 1 2.7572820253807038 1102 2 1.7025584597547017 1102 3 3.8659923529217366 1102 4 2.956846860223834 1102 5 3.1126406437137604 1102 6 1.6320838111089202 1102 7 0.864111297048197 1102 8 0.572465924825103 1102 9 0.6754864901067137 1102 10-11 0.6734183217023642 1102 12-13 0.594231533305674 1102 14-15 0.4238562410905189 1102 16-17 0.5854020156291924 1102 18-19 0.5087008991038786 1102 20-21 0.7359994905838931 1102 22-23 0.5871040297002565 1102 24-25 0.6224852893144757 1102 26-27 0.6649333871766316 1102 28-29 0.7692176911709865 1102 30-31 0.7898077216322932 1102 32-33 0.8514899791457466 1102 34-35 1.0536113467619757 1102 36-37 1.3286878199728136 1102 38-39 1.2120868696434215 1102 40-41 0.9697471691196 1102 42-43 0.8291977308829246 1102 44-45 0.9104296881872713 1102 46-47 0.7784441397713167 1102 48-49 0.8467186624416669 1102 50-51 0.8314937376564444 1102 52-53 0.9426320688701395 1102 54-55 0.9007219918383385 1102 56-57 0.7886019197841065 1102 58-59 0.6586903200874019 1102 60-61 0.6214257944947903 1102 62-63 0.6760712986045689 1102 64-65 0.15897659561613153 1102 66-67 0.4953438152276206 1102 68-69 0.2929043330795622 1102 70-71 0.14935653731069465 1102 72-73 0.2562246757948117 1102 74-75 0.25710186474054453 1102 76-77 0.12242372225563969 1102 78-79 0.27077107205075635 1102 80-81 0.2458155260614312 1102 82-83 0.25182951761499694 1102 84-85 0.2985990319139873 1102 86-87 0.3155503452171793 1102 88-89 0.19241774709546178 1102 90-91 0.24723411374963788 1102 92-93 0.17126139563245601 1102 94-95 0.17265094024073946 1102 96-97 0.27202889425362287 1102 98-99 0.23421121285893065 1102 100-101 -0.07947595684959197 1103 1 1.493535958585845 1103 2 0.7646869308688125 1103 3 1.8881756531033211 1103 4 -0.6036153611129791 1103 5 0.7523505851665391 1103 6 0.4195648876239346 1103 7 0.3019219758526077 1103 8 0.2084980302221595 1103 9 0.31648147101837765 1103 10-11 0.3879458775333191 1103 12-13 0.298216402122776 1103 14-15 0.3178809411597143 1103 16-17 0.28052396945009406 1103 18-19 0.28323072997292087 1103 20-21 0.3606317120793108 1103 22-23 0.3616754521240182 1103 24-25 0.3859787554662546 1103 26-27 0.3858120183920306 1103 28-29 0.519398901248266 1103 30-31 0.4839467949989853 1103 32-33 0.5150933343678545 1103 34-35 0.6142049091182855 1103 36-37 0.720072900823844 1103 38-39 0.6337684464813265 1103 40-41 0.4715276669884503 1103 42-43 0.31367395321044 1103 44-45 0.30662175853408513 1103 46-47 0.3409607260789542 1103 48-49 0.3674898623327465 1103 50-51 0.26479866779292394 1103 52-53 0.33821262882258907 1103 54-55 0.29281831192479046 1103 56-57 0.2767688140936997 1103 58-59 0.14858740730594633 1103 60-61 0.05689315623057212 1103 62-63 0.16907136436086745 1103 64-65 -0.17973738368149128 1103 66-67 -0.015797128901134272 1103 68-69 -0.22339433945007414 1103 70-71 0.2019553857685814 1103 72-73 0.5617646439850894 1103 74-75 0.5936917996492319 1103 76-77 0.032254975269278674 1103 78-79 0.4302480896677885 1103 80-81 0.5327049767385041 1103 82-83 0.4979911814700948 1103 84-85 0.4707494678578463 1103 86-87 0.5419863621587488 1103 88-89 0.45063919501948746 1103 90-91 0.46801016421115094 1103 92-93 0.40267717697727434 1103 94-95 0.31814480204325335 1103 96-97 0.38789578718131423 1103 98-99 0.3367516411578073 1103 100-101 0.0410351161309066 1216 1 0.9950936618906816 1216 2 0.327389073026481 1216 3 1.0157546269107627 1216 4 -0.518756371243704 1216 5 1.1915647925623034 1216 6 0.593634026296499 1216 7 0.26309175512126615 1216 8 0.1982304766562848 1216 9 0.21498870860292385 1216 10-11 0.2633507892639173 1216 12-13 0.21243397121973828 1216 14-15 0.16415590941003444 1216 16-17 0.18493166821725282 1216 18-19 0.14821698761803503 1216 20-21 0.2882441153561217 1216 22-23 0.12361330616502642 1216 24-25 0.22043110778672315 1216 26-27 0.2396923294515645 1216 28-29 0.3194670729852547 1216 30-31 0.294110409043185 1216 32-33 0.25751909720464283 1216 34-35 0.3946640753048811 1216 36-37 0.5790353172687119 1216 38-39 0.5059724807648607 1216 40-41 0.2562074709805131 1216 42-43 0.1403251460777355 1216 44-45 0.10361128712681733 1216 46-47 0.14967946841688473 1216 48-49 0.281723641930526 1216 50-51 0.15634197873725242 1216 52-53 0.14644694179154527 1216 54-55 0.0910163130650119 1216 56-57 0.07457319058453038 1216 58-59 0.06883540546338907 1216 60-61 -0.030525162784478255 1216 62-63 -0.023598453635344185 1216 64-65 -0.31699830245032246 1216 66-67 -0.160722300168878 1216 68-69 -0.4599874215591768 1216 70-71 0.15291047376450706 1216 72-73 0.49961329826204093 1216 74-75 0.4500573144923017 1216 76-77 0.025531864416205252 1216 78-79 0.4267327978955642 1216 80-81 0.5598580421419932 1216 82-83 0.5465940223075094 1216 84-85 0.5980858024525411 1216 86-87 0.5344847653613343 1216 88-89 0.5318777320105355 1216 90-91 0.5121250883419002 1216 92-93 0.5172776756803401 1216 94-95 0.4538524878409902 1216 96-97 0.4674333374646835 1216 98-99 0.43013421738249846 1216 100-101 0.11918753043761754 1113 1 2.3707254322100155 1113 2 1.2689898102462394 1113 3 2.531022234519959 1113 4 3.25109724700652 1113 5 2.8908445284943767 1113 6 1.2353199884837593 1113 7 0.5602818075766862 1113 8 0.37009713791385934 1113 9 0.258604530176882 1113 10-11 0.16835000144430268 1113 12-13 0.1057754353029452 1113 14-15 0.1484887859830124 1113 16-17 0.12282530746822573 1113 18-19 0.1324218860918691 1113 20-21 0.22240543648787536 1113 22-23 0.09570067650828662 1113 24-25 0.07552853744653731 1113 26-27 0.09480471051081452 1113 28-29 0.1135073545145957 1113 30-31 0.2525387197097544 1113 32-33 0.3920111967643649 1113 34-35 0.3843153908304302 1113 36-37 0.24129737653821337 1113 38-39 0.2618514314357796 1113 40-41 0.15889300156631947 1113 42-43 0.029757656049412162 1113 44-45 0.2012819900174776 1113 46-47 0.1899821975356062 1113 48-49 0.11702420212397158 1113 50-51 0.024278834622435852 1113 52-53 0.12877499097047718 1113 54-55 0.033628941062005424 1113 56-57 0.06255768692044228 1113 58-59 0.021375428107965888 1113 60-61 -0.17286611999472967 1113 62-63 -0.04632119743359908 1113 64-65 -0.4663079202793412 1113 66-67 -0.1922368856456842 1113 68-69 -0.3351145499903794 1113 70-71 0.22813183414104543 1113 72-73 0.6044343517471695 1113 74-75 0.6512982823306857 1113 76-77 0.12123521717886732 1113 78-79 0.5508565674483386 1113 80-81 0.6548169801309207 1113 82-83 0.7089477729788385 1113 84-85 0.7077535555748078 1113 86-87 0.644646423049636 1113 88-89 0.6493384955394568 1113 90-91 0.6159227159318057 1113 92-93 0.6531985549508903 1113 94-95 0.6008169953579028 1113 96-97 0.47608665283484086 1113 98-99 0.41189802962625777 1113 100-101 0.16267760268771525 1112 1 2.13546386893049 1112 2 1.1409645669331034 1112 3 1.6716348474397584 1112 4 2.9497559063733547 1112 5 2.347123709724059 1112 6 0.9939102933088577 1112 7 0.4790183596090287 1112 8 0.3062516579882697 1112 9 0.17953272602176185 1112 10-11 0.14127408977330447 1112 12-13 0.04910152506201371 1112 14-15 -0.05107557097951343 1112 16-17 -0.0318158330807492 1112 18-19 0.07039047912621044 1112 20-21 0.1531823052394401 1112 22-23 2.70945358217034E-4 1112 24-25 0.0754330472872553 1112 26-27 0.03533184143128665 1112 28-29 -0.08072337449055311 1112 30-31 -0.0024522300087141957 1112 32-33 0.16356052544229982 1112 34-35 0.24559121382009153 1112 36-37 0.2795772124274407 1112 38-39 0.22722095304233392 1112 40-41 0.16017999241469028 1112 42-43 0.013337913343690389 1112 44-45 0.058983282817798965 1112 46-47 -0.061002334416677684 1112 48-49 -0.09065720934232502 1112 50-51 -0.11932589039783892 1112 52-53 -0.07696147022625865 1112 54-55 -0.04186681979992102 1112 56-57 -0.11279910392693182 1112 58-59 -0.24198039316770803 1112 60-61 -0.20508702372382714 1112 62-63 -0.22717664332782306 1112 64-65 -0.2010010194974896 1112 66-67 -0.17602867842445846 1112 68-69 -0.2348565291572129 1112 70-71 0.04116616625607605 1112 72-73 0.35463902133434644 1112 74-75 0.45086682838225656 1112 76-77 -0.05570040013446942 1112 78-79 0.4049414151622095 1112 80-81 0.47228852288644774 1112 82-83 0.46841286720128394 1112 84-85 0.4833489856708546 1112 86-87 0.39121226582252433 1112 88-89 0.37621534474989815 1112 90-91 0.46471451718835155 1112 92-93 0.4573089922276381 1112 94-95 0.42157852247108707 1112 96-97 0.39715727935344347 1112 98-99 0.3669909998571761 1112 100-101 0.25074809194872927 1115 1 1.5049475347199746 1115 2 0.8305225185814571 1115 3 0.13350195756946093 1115 4 0.11712652904873622 1115 5 1.7097797850134882 1115 6 0.7553172949578979 1115 7 0.3171677567875051 1115 8 0.15230184640721944 1115 9 0.13361442279112623 1115 10-11 0.14838190202162593 1115 12-13 -0.005972770694398832 1115 14-15 -0.054837967947207744 1115 16-17 -0.064875458025206 1115 18-19 0.0221827310778977 1115 20-21 0.03773739447894542 1115 22-23 -0.13103801806582283 1115 24-25 -0.05980474043938244 1115 26-27 -0.05195940367385532 1115 28-29 -0.04636817090946721 1115 30-31 0.08080207274787199 1115 32-33 0.07454195430843669 1115 34-35 0.0610865777994789 1115 36-37 0.30775140707413584 1115 38-39 0.32854422811806927 1115 40-41 0.13554144215326147 1115 42-43 0.0248934405312653 1115 44-45 1.1445882608285274E-4 1115 46-47 0.051878471888919364 1115 48-49 0.038022690338195275 1115 50-51 -0.037841529183957334 1115 52-53 0.021712123793754756 1115 54-55 -0.12658165581282788 1115 56-57 -0.16843828025145058 1115 58-59 -0.02996511078288222 1115 60-61 -0.06795301175247914 1115 62-63 0.01113932824678443 1115 64-65 -0.20700428691493755 1115 66-67 -0.017609100429474722 1115 68-69 -0.0860573740025572 1115 70-71 0.03390904409589268 1115 72-73 0.28424160871709603 1115 74-75 0.34476329632524383 1115 76-77 0.13206980981390615 1115 78-79 0.3610614584527774 1115 80-81 0.47661503147003614 1115 82-83 0.4583107893766005 1115 84-85 0.4882247805837956 1115 86-87 0.4677056792512282 1115 88-89 0.5004995651954793 1115 90-91 0.47386078110174523 1115 92-93 0.4510194762149382 1115 94-95 0.48527581892090055 1115 96-97 0.45972010071658787 1115 98-99 0.3660366376323374 1115 100-101 0.3323086551354457 1114 1 1.9791813943398076 1114 2 1.0233617391462246 1114 3 3.4606499312024717 1114 4 0.5501304434632814 1114 5 1.187776972997014 1114 6 0.581236230778515 1114 7 0.26490853898108924 1114 8 0.12747199002755139 1114 9 -0.09561886976877076 1114 10-11 -0.12499549989485814 1114 12-13 -0.01915028106297001 1114 14-15 -0.07012572094932068 1114 16-17 -0.16010977118600067 1114 18-19 -0.09524576193959433 1114 20-21 -0.12851220818362918 1114 22-23 -0.025901485054760087 1114 24-25 -0.15016053018536724 1114 26-27 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-0.7245801145667627 1213 42-43 -0.4950848803888732 1213 44-45 -0.5911571924191463 1213 46-47 -0.6843691489426718 1213 48-49 -0.7221061203516399 1213 50-51 -0.6202950711934179 1213 52-53 -0.4754593123097308 1213 54-55 -0.3587955682714892 1213 56-57 -0.26068642743379655 1213 58-59 -0.3912135443943612 1213 60-61 -0.1338255570434015 1213 62-63 -0.1609742609856326 1213 64-65 -0.08244477953385143 1213 66-67 -0.11951050304016064 1213 68-69 0.3621046358786302 1213 70-71 1.467845889234212 1213 72-73 0.6228467662594745 1213 74-75 0.42052284702829823 1213 76-77 0.9785217169017706 1213 78-79 0.46514773229514716 1213 80-81 0.3425852391155324 1213 82-83 0.3221694427410817 1213 84-85 0.30560061483310363 1213 86-87 0.3344331106276499 1213 88-89 0.3068451281345439 1213 90-91 0.26077012482565465 1213 92-93 0.32608263595474796 1213 94-95 0.40775763876780147 1213 96-97 0.3838004766047085 1213 98-99 0.37367695993899375 1213 100-101 0.7429514811637574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7287.0 3 371.0 4 630.0 5 1084.0 6 1983.0 7 4352.0 8 7753.0 9 11672.0 10 15928.0 11 21611.0 12 28296.0 13 36396.0 14 40492.0 15 43376.0 16 48171.0 17 55804.0 18 67879.0 19 85729.0 20 109248.0 21 138518.0 22 173892.0 23 226799.0 24 312697.0 25 465854.0 26 627193.0 27 696409.0 28 286262.0 29 327028.0 30 407596.0 31 510044.0 32 640176.0 33 817591.0 34 1075933.0 35 1463870.0 36 2076261.0 37 2813238.0 38 2089585.0 39 262861.0 40 131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.308062462376864 26.469391722566133 12.21233107485121 37.0102147402058 2 13.363374700372836 40.63029992405799 21.284638775427293 24.721686600141883 3 10.20355317566317 15.144644526492312 42.51289860129654 32.13890369654797 4 12.558902290832172 23.340496872046444 12.952540618389666 51.148060218731715 5 27.245878258065204 26.34556694381406 15.821920248301396 30.58663454981934 6 26.533905358583592 29.985856868812384 18.175814201918712 25.304423570685312 7 8.05305 43.43655625 28.2068875 20.30350625 8 8.676291810315382 44.63343647728317 20.000282500547346 26.689989211854098 9 21.4370575898161 39.90814624425914 20.809257550578597 17.84553861534616 10-11 14.729829966228747 42.42002405250301 25.082268760283593 17.76787722098465 12-13 7.335462958466435 47.739362358710146 20.30301376893836 24.622160913885057 14-15 8.19989262493289 42.373260858288035 20.33852833658021 29.088318180198865 16-17 14.657020907020907 39.58118645618646 20.582917457917457 25.178875178875177 18-19 13.058430068758629 29.79967663035063 27.757483450500846 29.384409850389897 20-21 23.573454598340913 29.94848624678039 27.754504859656553 18.723554295222144 22-23 6.642710620508241 48.507455970147994 21.221472729026136 23.628360680317627 24-25 6.744109375 35.132553125 27.307834375000002 30.815503125 26-27 7.184184824011551 34.74198342137396 25.679792229987015 32.39403952462747 28-29 13.704262118422013 41.48338972347451 20.117197576910907 24.695150581192568 30-31 7.183532147941518 29.818141227267652 32.069741508717684 30.928585116073137 32-33 6.785669386156505 41.886378926848025 22.086383320598436 29.241568366397036 34-35 13.277142152944691 36.05598588821516 22.150970310207953 28.515901648632198 36-37 6.989412517023069 41.453615078295265 23.05143722536877 28.505535179312897 38-39 6.826029191567061 40.98635640411819 29.719326518644767 22.468287885669984 40-41 6.873371146591861 41.14331403732769 29.498376041140396 22.48493877494005 42-43 7.245578778461627 34.31236613237642 29.781947301915608 28.660107787246346 44-45 13.708785110435514 34.53922962357098 23.138356760004896 28.613628505988604 46-47 7.441150676076604 40.5200814753976 29.186373268814215 22.852394579711575 48-49 14.112799842345957 33.94880088066223 29.206339730397207 22.7320595465946 50-51 6.931054170020172 33.77844061470224 23.105495451448817 36.185009763828766 52-53 13.446794568843522 34.16190148415439 30.306029516459095 22.085274430542995 54-55 6.847566254990895 33.92317909205495 29.943013979659483 29.28624067329467 56-57 7.240148700743504 33.617564962824815 29.59188858444292 29.55039775198876 58-59 13.700049018869484 33.973226559739736 30.295533220362223 22.03119120102855 60-61 7.27958599098628 34.83864925381133 29.39881686442416 28.482947890778227 62-63 13.537617632347388 35.505051669826365 29.07855002360319 21.878780674223062 64-65 6.795725209847451 48.7287495890989 22.40116365752741 22.074361543526237 66-67 6.796571701112023 48.68216859008132 22.624523870380553 21.896735838426107 68-69 6.919888339078797 48.40909937506984 22.678874600715456 21.9921376851359 70-71 7.133446185652952 47.03511097976361 23.140017199411012 22.691425635172426 72-73 7.530525000000001 45.15748125 23.7112375 23.60075625 74-75 7.923674889840887 43.702862472882295 24.105510622878253 24.267952014398567 76-77 8.155770306814572 42.20004221968015 24.717835189806525 24.926352283698748 78-79 8.449551845782624 41.57300758420598 24.858344967215857 25.119095602795532 80-81 8.777633017711944 40.8186395269148 25.058218442835173 25.34550901253808 82-83 8.964322201453848 40.393377298175956 24.824047294727155 25.81825320564304 84-85 9.213959636945889 39.84401743282736 25.141799066733363 25.800223863493393 86-87 9.405843115562465 39.79030091579209 25.142980696625482 25.660875272019968 88-89 9.751841156940435 39.154545932954726 25.098337536876574 25.995275373228267 90-91 9.926666820000115 39.021116765837576 24.948949961712472 26.10326645244984 92-93 10.169995709997854 38.81383190691595 24.879431189715596 26.136741193370593 94-95 10.351161645056852 38.98860948718567 24.639771469742904 26.020457398014575 96-97 10.561616942687726 38.45021137952842 24.79106347460938 26.197108203174473 98-99 10.871116239175802 38.44767785992619 24.49856551306982 26.182640387828194 100-101 11.323534039552573 38.34353095265722 24.111593360818723 26.221341646971485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 16.5 2 19.5 3 29.0 4 48.0 5 74.0 6 102.5 7 139.5 8 218.5 9 431.5 10 856.5 11 1751.0 12 3422.5 13 6166.0 14 10285.0 15 15943.5 16 23601.0 17 33675.0 18 47521.0 19 66975.0 20 93727.5 21 129671.5 22 177042.5 23 237413.5 24 309493.5 25 389817.0 26 471566.5 27 547654.0 28 611765.0 29 660433.5 30 690721.0 31 701264.5 32 699765.5 33 687044.0 34 656260.0 35 615951.0 36 577602.5 37 547187.0 38 525806.0 39 510622.5 40 501801.0 41 499342.5 42 503413.5 43 503595.0 44 491290.5 45 468139.5 46 434353.5 47 391771.0 48 342870.0 49 292111.5 50 246831.5 51 206663.0 52 168956.0 53 138353.5 54 115690.0 55 97357.0 56 82581.0 57 70365.5 58 60399.5 59 52097.5 60 44857.5 61 38607.0 62 32938.0 63 28066.5 64 23698.0 65 19997.0 66 16909.5 67 14079.5 68 11628.5 69 9617.5 70 7925.5 71 6463.0 72 5296.0 73 4276.5 74 3399.0 75 2703.5 76 2116.5 77 1624.5 78 1243.0 79 925.5 80 720.5 81 565.5 82 425.0 83 340.0 84 270.0 85 215.5 86 182.0 87 171.0 88 149.5 89 120.5 90 94.5 91 77.5 92 62.5 93 46.5 94 33.5 95 27.0 96 19.5 97 15.0 98 11.0 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0158125 2 0.0059375 3 0.00248125 4 0.0145 5 5.625000000000001E-4 6 4.375E-5 7 0.0 8 1.9375000000000002E-4 9 6.2499999999999995E-6 10-11 1.2499999999999999E-5 12-13 6.2499999999999995E-6 14-15 6.25E-5 16-17 9.999999999999999E-5 18-19 2.78125E-4 20-21 6.2499999999999995E-6 22-23 1.8750000000000002E-5 24-25 0.0 26-27 6.2499999999999995E-6 28-29 6.5625E-5 30-31 1.2499999999999999E-5 32-33 9.375000000000001E-6 34-35 2.4687499999999997E-4 36-37 1.34375E-4 38-39 1.5625E-5 40-41 3.53125E-4 42-43 0.00324375 44-45 0.005134375 46-47 0.005090625 48-49 0.018421875 50-51 0.015209374999999999 52-53 0.002190625 54-55 6.9375E-4 56-57 5.0E-4 58-59 2.4375E-4 60-61 3.65625E-4 62-63 2.6875E-4 64-65 6.46875E-4 66-67 0.0017906250000000001 68-69 8.250000000000001E-4 70-71 2.09375E-4 72-73 0.0 74-75 4.71875E-4 76-77 0.0022031250000000002 78-79 2.4375E-4 80-81 0.0021562499999999997 82-83 0.0015375 84-85 4.4375E-4 86-87 1.59375E-4 88-89 3.7500000000000003E-5 90-91 7.500000000000001E-5 92-93 4.9999999999999996E-5 94-95 1.125E-4 96-97 0.001403125 98-99 1.78125E-4 100-101 6.59375E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.85796066547294 #Duplication Level Percentage of deduplicated Percentage of total 1 89.98690168446261 61.963145365965076 2 6.833732229674451 9.411137301385963 3 1.345591450993855 2.779640496129946 4 0.60332537139593 1.6617501876825123 5 0.38813328250679585 1.3363033149906924 6 0.1788665197630644 0.7389830269329077 7 0.12178469668905892 0.587009209799024 8 0.10856636418009764 0.5980526747445261 9 0.06493926758170651 0.40244269795071924 >10 0.33683869755616247 3.7752909360848492 >50 0.01790937662283059 0.8076769117860347 >100 0.01089075334215901 1.4999623374290978 >500 0.0015190179355377832 0.6975707523567398 >1k 8.170232591660331E-4 1.0715573572390498 >5k 8.392823675741131E-5 0.39111829036978624 >10k+ 1.0033579982045621E-4 12.278359139153121 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 1581490 9.8843125 TruSeq Adapter, Index 6 (100% over 50bp) CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT 104410 0.6525625 TruSeq Adapter, Index 6 (100% over 50bp) GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 92242 0.5765125 TruSeq Adapter, Index 6 (98% over 50bp) GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 41409 0.25880625 TruSeq Adapter, Index 6 (98% over 50bp) GAACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 34786 0.21741249999999998 TruSeq Adapter, Index 6 (98% over 50bp) GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT 23909 0.14943125 TruSeq Adapter, Index 6 (100% over 50bp) GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 22256 0.1391 TruSeq Adapter, Index 6 (98% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.625E-4 0.0 0.0 2 9.375E-4 0.0 6.25E-6 3 0.0010125 0.0 6.25E-6 4 0.00105 0.0 6.25E-6 5 0.0012125 0.0 6.25E-6 6 0.00138125 0.0 6.25E-6 7 0.0017125 0.0 6.25E-6 8 0.00243125 0.0 6.25E-6 9 0.0026125 0.0 1.25E-5 10-11 0.003975 0.0 1.25E-5 12-13 0.006034375 0.0 1.25E-5 14-15 0.00938125 0.0 1.25E-5 16-17 0.011890625 0.0 1.25E-5 18-19 0.013059375 0.0 1.875E-5 20-21 0.014593749999999999 0.0 1.875E-5 22-23 0.02146875 0.0 1.875E-5 24-25 0.035824999999999996 0.0 2.1875E-5 26-27 0.041471875 0.0 2.5E-5 28-29 0.043187500000000004 0.0 2.5E-5 30-31 0.04450625 0.0 2.5E-5 32-33 0.046293749999999995 0.0 2.5E-5 34-35 0.047740624999999995 0.0 2.5E-5 36-37 0.049390625 0.0 2.5E-5 38-39 0.051328125 6.25E-6 2.5E-5 40-41 0.053881250000000006 6.25E-6 2.5E-5 42-43 0.057153125 6.25E-6 2.8125E-5 44-45 0.0612375 1.25E-5 3.4374999999999995E-5 46-47 0.06671250000000001 1.875E-5 3.75E-5 48-49 0.073459375 2.1875E-5 4.375E-5 50-51 0.08220625000000001 2.5E-5 4.375E-5 52-53 0.0936125 2.5E-5 4.375E-5 54-55 0.10874687499999999 2.5E-5 4.6875E-5 56-57 0.12808124999999998 3.125E-5 5.0E-5 58-59 0.154009375 3.125E-5 5.0E-5 60-61 0.1884625 3.125E-5 5.0E-5 62-63 0.23220625 3.125E-5 5.0E-5 64-65 0.289328125 3.125E-5 5.3125000000000004E-5 66-67 0.36253749999999996 3.4374999999999995E-5 5.625E-5 68-69 0.45414375 3.75E-5 5.625E-5 70-71 0.5692062499999999 3.75E-5 5.625E-5 72-73 0.71066875 3.75E-5 6.25E-5 74-75 0.883740625 3.75E-5 6.562500000000001E-5 76-77 1.089978125 3.75E-5 6.875E-5 78-79 1.335825 3.75E-5 6.875E-5 80-81 1.623865625 4.0625E-5 6.875E-5 82-83 1.966378125 4.375E-5 7.1875E-5 84-85 2.3650343749999996 4.375E-5 8.125E-5 86-87 2.8285187499999997 4.375E-5 8.125E-5 88-89 3.3599125 5.0E-5 8.4375E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGGA 12615 0.0 83.167755 1 CCGGAAG 13270 0.0 81.47844 3 ACCGGAA 13720 0.0 79.018974 2 GAGCACA 289925 0.0 71.88395 9 AGAGCAC 291560 0.0 71.503426 8 GAAGAGC 296500 0.0 70.41943 6 AAGAGCA 297370 0.0 70.17508 7 GGAAGAG 296110 0.0 68.78003 5 TCCGAAG 5570 0.0 68.65656 3 CGGAAGA 291430 0.0 68.261856 4 TCGGAAG 265805 0.0 68.10741 3 ATCGGAA 272390 0.0 66.47575 2 AATCATA 224110 0.0 65.476234 5 TAAATCA 208495 0.0 65.16688 3 ATAAATC 209350 0.0 64.97988 2 GATCGGA 274470 0.0 64.743576 1 ACGGAAG 7100 0.0 64.633865 3 GAACGGA 6770 0.0 64.43134 1 GATCCGA 5300 0.0 64.37122 1 TCATAAA 237355 0.0 64.11157 7 >>END_MODULE