##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_GCCAAT_L001_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8792684
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.913421885740462	31.0	26.0	31.0	16.0	34.0
2	29.725964904459207	31.0	30.0	33.0	25.0	34.0
3	26.49972113179548	30.0	23.0	31.0	10.0	34.0
4	30.561279809441576	35.0	28.0	35.0	19.0	37.0
5	32.512148850112204	35.0	32.0	37.0	22.0	37.0
6	34.09768029875747	35.0	33.0	37.0	30.0	37.0
7	34.82608711970088	35.0	35.0	37.0	32.0	37.0
8	35.04551056309996	36.0	35.0	37.0	32.0	37.0
9	36.77256228018658	39.0	37.0	39.0	32.0	39.0
10-11	36.96341373123383	39.0	37.0	39.0	33.0	39.0
12-13	36.980409508632405	39.0	37.0	39.0	33.0	39.0
14-15	38.23659817639301	40.0	37.5	41.0	33.0	41.0
16-17	38.17778592975706	40.0	37.5	41.0	33.0	41.0
18-19	38.19324400831418	40.0	37.5	41.0	33.0	41.0
20-21	38.16847506404188	40.0	37.5	41.0	33.0	41.0
22-23	38.26750551936132	40.0	38.0	41.0	34.0	41.0
24-25	38.12986955973852	40.0	37.5	41.0	33.0	41.0
26-27	37.808070266143986	40.0	37.0	41.0	32.5	41.0
28-29	37.738328364808744	40.0	37.0	41.0	32.5	41.0
30-31	37.733826724581476	40.0	37.0	41.0	32.5	41.0
32-33	37.6578408253953	40.0	37.0	41.0	32.0	41.0
34-35	36.9371301754959	40.0	36.0	41.0	30.0	41.0
36-37	36.87026162887237	40.0	36.0	41.0	30.0	41.0
38-39	36.85851481754604	40.0	36.0	41.0	30.0	41.0
40-41	37.31584172705399	40.0	37.0	41.0	31.0	41.0
42-43	37.22986593172233	40.0	37.0	41.0	31.0	41.0
44-45	37.11479810942825	40.0	36.5	41.0	31.0	41.0
46-47	36.9433938487952	39.5	36.0	41.0	30.5	41.0
48-49	36.913855882913566	40.0	36.0	41.0	30.5	41.0
50-51	36.84149754500446	40.0	36.0	41.0	30.0	41.0
52-53	36.49345666237977	39.0	35.0	40.5	30.0	41.0
54-55	36.410299630920434	39.0	35.0	40.0	30.0	41.0
56-57	36.25852271047157	39.0	35.0	40.0	29.0	41.0
58-59	35.86209808063158	38.5	35.0	40.0	28.5	41.0
60-61	35.54637082374393	38.0	34.0	40.0	28.0	41.0
62-63	35.19110188652293	37.5	34.0	40.0	28.0	41.0
64-65	34.67764712117483	37.0	34.0	39.5	27.5	41.0
66-67	34.4500108840486	37.0	34.0	39.0	28.0	41.0
68-69	33.56155731287511	35.5	33.5	39.0	25.0	40.5
70-71	30.681926758655266	35.0	30.5	38.5	2.0	40.0
72-73	29.493765441815036	35.0	29.5	37.0	2.0	40.0
74-75	29.12692267799002	35.0	29.0	37.0	2.0	39.0
76-77	27.297813727867393	33.0	26.0	35.0	2.0	37.0
78-79	28.506707053272926	34.5	29.0	36.0	2.0	38.5
80-81	28.502177264644107	35.0	29.0	36.0	2.0	37.0
82-83	28.301015366866363	34.5	29.0	35.0	2.0	37.0
84-85	28.085459172648534	34.0	29.0	35.0	2.0	36.5
86-87	27.89687096681741	34.0	29.0	35.0	2.0	36.0
88-89	27.701401301354625	34.0	29.0	35.0	2.0	36.0
90-91	27.5576496892189	34.0	29.0	35.0	2.0	36.0
92-93	27.41386543631046	34.0	29.0	35.0	2.0	36.0
94-95	27.248392015452847	34.0	29.0	35.0	2.0	35.0
96-97	27.050165740062987	34.0	27.5	35.0	2.0	35.0
98-99	26.828579475846055	34.0	27.0	35.0	2.0	35.0
100-101	25.887070034587843	33.0	23.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	-4.053240737087648
2306	2	-2.273575218487803
2306	3	-2.651175650696949
2306	4	-0.039727322475211935
2306	5	-5.286273507306557
2306	6	-2.4338516978824067
2306	7	-1.266970954697996
2306	8	-0.7058053231582235
2306	9	-0.9233151160983226
2306	10-11	-0.8199361469266364
2306	12-13	-0.8590978532909475
2306	14-15	-0.8009224271842967
2306	16-17	-0.9666898474973777
2306	18-19	-0.8291263475283088
2306	20-21	-1.149228823838449
2306	22-23	-1.0435759290212303
2306	24-25	-3.609096471426575
2306	26-27	-2.993624968205559
2306	28-29	-1.5245500026651655
2306	30-31	-1.258147064301106
2306	32-33	-1.374992450614947
2306	34-35	-1.4059539901887987
2306	36-37	-1.6128604564962288
2306	38-39	-1.521752222856044
2306	40-41	-1.4373347650717463
2306	42-43	-1.079899570664871
2306	44-45	-1.0484010586695192
2306	46-47	-0.977341046440479
2306	48-49	-1.180713506832781
2306	50-51	-1.2604608880935615
2306	52-53	-1.1264026180923636
2306	54-55	-0.9526830085037261
2306	56-57	-0.9764737813707143
2306	58-59	-0.9713337529221207
2306	60-61	-0.8912043369053464
2306	62-63	-0.8770089809039234
2306	64-65	-0.7506495789009975
2306	66-67	-0.7468869478234481
2306	68-69	-0.6432889331794058
2306	70-71	0.9598278409799867
2306	72-73	0.18628708339000966
2306	74-75	-0.006299707874010352
2306	76-77	0.019024640213483934
2306	78-79	0.042872385006102576
2306	80-81	0.0871361378712372
2306	82-83	0.1104381533942913
2306	84-85	-0.012126480438780618
2306	86-87	0.04519311986108576
2306	88-89	0.11931439979609593
2306	90-91	0.0777782510591365
2306	92-93	0.06340959892147424
2306	94-95	-0.05308865200552404
2306	96-97	-0.0016500784948831893
2306	98-99	0.05742619534800397
2306	100-101	0.37726797160911474
2201	1	3.594069032330161
2201	2	2.230705613374461
2201	3	4.355392991417968
2201	4	4.0285894182104975
2201	5	3.0666356026788364
2201	6	1.5909099591579476
2201	7	0.877623381502957
2201	8	0.5937678346113628
2201	9	0.7093057771049658
2201	10-11	0.5882983550966969
2201	12-13	0.5327611135873127
2201	14-15	0.520955589598465
2201	16-17	0.5780741613323102
2201	18-19	0.5200784682568624
2201	20-21	0.7318887596014463
2201	22-23	0.6734771049924646
2201	24-25	0.7025334624000763
2201	26-27	0.668862135989805
2201	28-29	0.7210325644532887
2201	30-31	0.6920035762293324
2201	32-33	0.6921590174933172
2201	34-35	0.8100946434996743
2201	36-37	0.14010233236157177
2201	38-39	0.34251175611399987
2201	40-41	0.5740606335846223
2201	42-43	0.45625480040338573
2201	44-45	0.5782705677112858
2201	46-47	0.5841741606101252
2201	48-49	0.5239393720751409
2201	50-51	0.5156442200421836
2201	52-53	0.5576683990969329
2201	54-55	0.40966515247097846
2201	56-57	0.2665968911999457
2201	58-59	0.1149603078578707
2201	60-61	-0.034085380195797654
2201	62-63	0.022856602761699207
2201	64-65	-0.37713913679199607
2201	66-67	-0.022706156723707238
2201	68-69	-0.2740285318877156
2201	70-71	-0.12082439087767938
2201	72-73	0.24913274985177125
2201	74-75	0.2247490888097623
2201	76-77	-0.4054500971586279
2201	78-79	0.04366975390583505
2201	80-81	0.14093665408694278
2201	82-83	0.1488394489192899
2201	84-85	0.15569800759161012
2201	86-87	0.1168575785578021
2201	88-89	0.09416041717714663
2201	90-91	0.10763370984022558
2201	92-93	0.10504697437388444
2201	94-95	0.11396777203647446
2201	96-97	0.07924515333100146
2201	98-99	0.045883943404938066
2201	100-101	-0.4286145767169387
2307	1	3.151688080914166
2307	2	1.9513437505302562
2307	3	4.512618405393855
2307	4	3.184074012490779
2307	5	2.702175882655105
2307	6	1.4703411396352593
2307	7	0.7021306748353808
2307	8	0.45334469260569676
2307	9	0.5855777631509227
2307	10-11	0.48010315283993066
2307	12-13	0.43714006821931406
2307	14-15	0.45711013901986064
2307	16-17	0.5719433527298463
2307	18-19	0.5153844143945463
2307	20-21	0.724948350282439
2307	22-23	0.6184548164327097
2307	24-25	0.6769012278817357
2307	26-27	0.7112862474870312
2307	28-29	0.7639474744170798
2307	30-31	0.6781839261687921
2307	32-33	0.7696080065279389
2307	34-35	0.9059900838304458
2307	36-37	0.8312896956031395
2307	38-39	0.8298040155050543
2307	40-41	0.7505790887591317
2307	42-43	0.6260514233266505
2307	44-45	0.729308018725277
2307	46-47	0.5797524946015571
2307	48-49	0.6675036517680937
2307	50-51	0.7051250967319618
2307	52-53	0.7401165346277381
2307	54-55	0.6730909966436656
2307	56-57	0.5608520337413481
2307	58-59	0.4217485845849325
2307	60-61	0.3144300900794903
2307	62-63	0.457895262286911
2307	64-65	0.009437105753235642
2307	66-67	0.35782549847349543
2307	68-69	0.06427824391936099
2307	70-71	-6.469094433825262E-5
2307	72-73	0.18330956351601557
2307	74-75	0.20401085674086872
2307	76-77	-0.09876804764170188
2307	78-79	0.08813505185923631
2307	80-81	0.14371365829144978
2307	82-83	0.16206993045948082
2307	84-85	0.11754546798112031
2307	86-87	0.15975710229399098
2307	88-89	0.08575838860749485
2307	90-91	0.1281712189445905
2307	92-93	0.09824226926644997
2307	94-95	0.060564093874091895
2307	96-97	0.11626629619818019
2307	98-99	0.11852028062781628
2307	100-101	-0.16503343248337998
2202	1	2.611047123362951
2202	2	1.6404180933646302
2202	3	3.313502940091219
2202	4	1.0181499735452526
2202	5	1.336869031768117
2202	6	0.7649993586623367
2202	7	0.42760829532438294
2202	8	0.28535759195742116
2202	9	0.46614857113272024
2202	10-11	0.3957567056117597
2202	12-13	0.4451088999059323
2202	14-15	0.39995552468971596
2202	16-17	0.5044884106586238
2202	18-19	0.48511189848270675
2202	20-21	0.6345836536714344
2202	22-23	0.5154315811388983
2202	24-25	0.6216504077215106
2202	26-27	0.5695299524559232
2202	28-29	0.6257299378421592
2202	30-31	0.5782373361667723
2202	32-33	0.6133155826359484
2202	34-35	0.7911979106562725
2202	36-37	0.8484741580928556
2202	38-39	0.8140255702231585
2202	40-41	0.6729920189096035
2202	42-43	0.5464526779671885
2202	44-45	0.551519405569401
2202	46-47	0.48483629645073023
2202	48-49	0.5652479462279203
2202	50-51	0.5292188860595886
2202	52-53	0.5150839040656905
2202	54-55	0.39844097567552694
2202	56-57	0.3613468211411188
2202	58-59	0.306254803649189
2202	60-61	0.15713210860099736
2202	62-63	0.21910181141269192
2202	64-65	-0.15118929358524724
2202	66-67	0.0923834448768659
2202	68-69	-0.2154248460652468
2202	70-71	-0.025051746337172887
2202	72-73	0.25547374197357087
2202	74-75	0.24252665407280105
2202	76-77	-0.12048560295495037
2202	78-79	0.1523654196884614
2202	80-81	0.2443567635811661
2202	82-83	0.24906110208742405
2202	84-85	0.19466159111931347
2202	86-87	0.1691682412450568
2202	88-89	0.0528806190886435
2202	90-91	0.08911980542710651
2202	92-93	0.15777172336083112
2202	94-95	0.14387564822929733
2202	96-97	0.15055062678128195
2202	98-99	0.1722300918209676
2202	100-101	-0.10921761140964037
2304	1	2.500646475849372
2304	2	1.41039149231759
2304	3	2.8912906894042294
2304	4	0.9084398197496633
2304	5	1.6065411248038117
2304	6	0.8815413603111182
2304	7	0.513878352227124
2304	8	0.3805218671052515
2304	9	0.5060546603202809
2304	10-11	0.4170091342708844
2304	12-13	0.391732118744919
2304	14-15	0.393125291867193
2304	16-17	0.4482275457667626
2304	18-19	0.45733438483198796
2304	20-21	0.5799809301814207
2304	22-23	0.5121868310058275
2304	24-25	0.5578916407588181
2304	26-27	0.5332935740235456
2304	28-29	0.5792735501586534
2304	30-31	0.5534688689752585
2304	32-33	0.5203727508345892
2304	34-35	0.7658508408881488
2304	36-37	0.8910812358266398
2304	38-39	0.8510104494325077
2304	40-41	0.66810592845016
2304	42-43	0.6356027064343195
2304	44-45	0.6136895250138252
2304	46-47	0.5696497967511647
2304	48-49	0.5598749142903614
2304	50-51	0.5413297005830273
2304	52-53	0.5939629752256153
2304	54-55	0.5218938406074187
2304	56-57	0.4582806423771615
2304	58-59	0.43163511580776515
2304	60-61	0.3295754870416019
2304	62-63	0.42036766294418726
2304	64-65	0.13313540633026832
2304	66-67	0.2960288743917303
2304	68-69	0.10665967969366363
2304	70-71	0.08790800268351617
2304	72-73	0.3604109525860686
2304	74-75	0.3943169056326177
2304	76-77	0.18016909805104575
2304	78-79	0.3406026522753258
2304	80-81	0.3717624323094384
2304	82-83	0.3541828023889515
2304	84-85	0.3338916258711855
2304	86-87	0.3253055500202926
2304	88-89	0.3239429724740752
2304	90-91	0.29044771348539555
2304	92-93	0.26929369942917347
2304	94-95	0.2801488039586353
2304	96-97	0.27974086332887893
2304	98-99	0.2807549557933342
2304	100-101	0.06693528710320962
2203	1	0.5239322842498382
2203	2	0.3645807007194506
2203	3	-0.7361136702206856
2203	4	-2.1091518021244653
2203	5	-0.18729483820904136
2203	6	-0.0205183620307281
2203	7	0.01996684637713031
2203	8	0.06569640959040868
2203	9	0.145522833347016
2203	10-11	0.1925378192118785
2203	12-13	0.2510465362223542
2203	14-15	0.3027985437852436
2203	16-17	0.33859608408827313
2203	18-19	0.2761046750993543
2203	20-21	0.32535849689256935
2203	22-23	0.3084645445200991
2203	24-25	0.42570954129589467
2203	26-27	0.43275816820924007
2203	28-29	0.34002834321691466
2203	30-31	0.3233082594502079
2203	32-33	0.33659349871425803
2203	34-35	0.5566377833214347
2203	36-37	0.5612777211005451
2203	38-39	0.5620744528493375
2203	40-41	0.4221694413052077
2203	42-43	0.332370569913941
2203	44-45	0.23242745436783707
2203	46-47	0.2534262748129237
2203	48-49	0.23744818280679425
2203	50-51	0.283336025337654
2203	52-53	0.25589433991358845
2203	54-55	0.24758225168054793
2203	56-57	0.22096503682877966
2203	58-59	0.19492489071922847
2203	60-61	0.10622407557441704
2203	62-63	0.19021976506613214
2203	64-65	0.07294804091574747
2203	66-67	0.07648048338817404
2203	68-69	0.05596105362624826
2203	70-71	0.03348114954285819
2203	72-73	0.4275275962823102
2203	74-75	0.4469462466444263
2203	76-77	0.1931949860951292
2203	78-79	0.342274689531024
2203	80-81	0.3682401481732924
2203	82-83	0.36789742066109454
2203	84-85	0.4168576204633254
2203	86-87	0.4334539988231789
2203	88-89	0.37152893608333315
2203	90-91	0.33658301174943617
2203	92-93	0.31883035425935446
2203	94-95	0.2774664980661896
2203	96-97	0.2715907868940377
2203	98-99	0.3190304791425369
2203	100-101	0.09935454951772016
2305	1	2.0535188167734937
2305	2	1.4147981730518495
2305	3	2.9367258728877417
2305	4	1.7768132599517692
2305	5	0.9677352624842754
2305	6	0.6692359684791711
2305	7	0.4320161505938529
2305	8	0.3135028391573016
2305	9	0.14132542975703188
2305	10-11	0.20138634085002138
2305	12-13	0.2759414719929296
2305	14-15	0.29066702829928204
2305	16-17	0.24652331712557896
2305	18-19	0.2778299419327368
2305	20-21	0.4100669576623872
2305	22-23	0.36455293616203477
2305	24-25	0.4490029882300135
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2214	78-79	-0.142548449716017
2214	80-81	-0.22306007017278517
2214	82-83	-0.23672140755618898
2214	84-85	-0.24742996446313015
2214	86-87	-0.21630281163645293
2214	88-89	-0.15463960254464482
2214	90-91	-0.16008579403990453
2214	92-93	-0.17193377644255747
2214	94-95	-0.1601831255999855
2214	96-97	-0.14703008189443167
2214	98-99	-0.14803940962188022
2214	100-101	-0.0015351358427437845
2213	1	-3.3237953379952963
2213	2	-1.5266340959171103
2213	3	-2.962351586355151
2213	4	-1.1596720782388097
2213	5	0.3744016998835704
2213	6	-0.14671093556030002
2213	7	-0.21354095515633986
2213	8	-0.4521013262710767
2213	9	-0.6002677075886709
2213	10-11	-0.4535856636979503
2213	12-13	-0.6320929660869794
2213	14-15	-0.6780596358932343
2213	16-17	-0.5896859720097254
2213	18-19	-0.5709687883233485
2213	20-21	-0.758386272304115
2213	22-23	-0.654717873596887
2213	24-25	-0.6396636538186016
2213	26-27	-0.5602777315842431
2213	28-29	-0.6881534659802284
2213	30-31	-0.6642988231715421
2213	32-33	-0.6547065622087374
2213	34-35	-1.0189139017214828
2213	36-37	-0.9732406885807237
2213	38-39	-0.9849058376602713
2213	40-41	-0.753287695503225
2213	42-43	-0.6713674598554888
2213	44-45	-0.7503071451484473
2213	46-47	-0.6957952693153118
2213	48-49	-0.6690113429299487
2213	50-51	-0.6707799873001363
2213	52-53	-0.6910624425398666
2213	54-55	-0.5377541224641647
2213	56-57	-0.5305849278146795
2213	58-59	-0.5321387377809614
2213	60-61	-0.4373170415863825
2213	62-63	-0.5138450032801245
2213	64-65	-0.34884487738737135
2213	66-67	-0.4404305233504644
2213	68-69	-0.18255085089060685
2213	70-71	0.9570229366705725
2213	72-73	-0.19751253264909963
2213	74-75	-0.47187076872742395
2213	76-77	-0.2251699602209527
2213	78-79	-0.35780768876579216
2213	80-81	-0.40923476134028647
2213	82-83	-0.39894577730527203
2213	84-85	-0.36968260822999355
2213	86-87	-0.37465322467208395
2213	88-89	-0.38966047427754447
2213	90-91	-0.39868856791016327
2213	92-93	-0.4018428973511732
2213	94-95	-0.3712591288561917
2213	96-97	-0.3873657430247519
2213	98-99	-0.4061985883531847
2213	100-101	-0.12416847162813838
2212	1	-4.168112002591712
2212	2	-5.822459773579116
2212	3	-0.5580380923850932
2212	4	1.995871224856291
2212	5	-4.218511383231405
2212	6	-1.941695075149788
2212	7	-1.3196451557944329
2212	8	-1.0322115369504488
2212	9	-1.32773051719456
2212	10-11	-1.1775254727396458
2212	12-13	-1.029909652208815
2212	14-15	-1.0225631915637408
2212	16-17	-1.2044499710031715
2212	18-19	-0.9800122866115188
2212	20-21	-1.4997628083699581
2212	22-23	-1.1867901953764104
2212	24-25	-1.0099350552294055
2212	26-27	-1.1239565763852042
2212	28-29	-1.5273794372886869
2212	30-31	-1.5354269406962544
2212	32-33	-1.2689477577422608
2212	34-35	-1.4628744522986494
2212	36-37	-1.7283018639025656
2212	38-39	-1.5433870567260968
2212	40-41	-1.5771797689279623
2212	42-43	-1.3773581567691906
2212	44-45	-1.5024017306092645
2212	46-47	-1.4662475072693724
2212	48-49	-1.4475255828553273
2212	50-51	-1.3783200175433308
2212	52-53	-1.599667875260515
2212	54-55	-1.402392011930992
2212	56-57	-1.457279606277929
2212	58-59	-1.2881625443460507
2212	60-61	-1.0218573444792725
2212	62-63	-1.4623839292950578
2212	64-65	-0.9413515926852938
2212	66-67	-0.9881985259194508
2212	68-69	-0.6111839267329913
2212	70-71	1.087261593727959
2212	72-73	0.29607531907438656
2212	74-75	0.08049726750754971
2212	76-77	0.3097502538407646
2212	78-79	0.07156616897038859
2212	80-81	-0.02544291406742971
2212	82-83	0.05225301341626576
2212	84-85	0.020539126958531995
2212	86-87	0.009601114011783096
2212	88-89	-0.05570902856718618
2212	90-91	-0.1338999630207489
2212	92-93	-0.1270867614157538
2212	94-95	-0.2033441632775883
2212	96-97	-0.057696508974238725
2212	98-99	-0.07563045908513644
2212	100-101	0.0695039800752113
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10902.0
3	294.0
4	527.0
5	854.0
6	1325.0
7	2423.0
8	4061.0
9	6050.0
10	8223.0
11	10653.0
12	14279.0
13	17869.0
14	19799.0
15	20754.0
16	22846.0
17	25516.0
18	30377.0
19	38193.0
20	48037.0
21	61674.0
22	78677.0
23	104059.0
24	146920.0
25	230377.0
26	346148.0
27	479728.0
28	169386.0
29	162604.0
30	199873.0
31	247371.0
32	309040.0
33	393685.0
34	525549.0
35	739546.0
36	1106770.0
37	1621115.0
38	1377225.0
39	209807.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.349576089262083	26.857157804887898	12.259217620401852	36.53404848544817
2	13.336102319340437	40.91994555894489	21.08373957322662	24.660212548488058
3	10.589552333876885	15.246124916283268	42.357015007134116	31.807307742705724
4	12.73441336190428	23.08058038047559	13.2352504600656	50.94975579755453
5	27.71720191687419	26.293899175765933	16.230559895039583	29.758339012320295
6	27.172423785742662	29.730085676959206	18.035504309892357	25.061986227405775
7	7.991149441039775	43.8718409037533	27.94587306578836	20.191136589418566
8	8.728153997118513	44.98807190159455	19.75874488381477	26.525029217472163
9	21.972242820164045	39.53984450702175	20.6278518040006	17.860060868813605
10-11	14.950400810491995	42.630691606794926	24.846730531883097	17.572177050829985
12-13	7.293967348309118	47.758574059979864	20.10337230361059	24.844086288100424
14-15	8.189783070715292	42.36961968710112	20.199265730213572	29.241331511970014
16-17	14.928741606586884	39.23914695667142	20.424623495204848	25.40748794153685
18-19	13.02864027826872	29.617776297754077	27.836811919771193	29.516771504206012
20-21	23.762927945607466	29.716876896535606	27.86551240310545	18.654682754751484
22-23	6.5817663550992656	48.83592558344464	21.054521328849024	23.527786732607066
24-25	6.7282447859040015	34.90181235424309	27.40061775252925	30.96932510732365
26-27	7.182936348690561	34.46234273208101	25.499453892235458	32.85526702699297
28-29	13.917442174549693	41.5594319102657	19.920040943404274	24.60308497178033
30-31	7.169897349411484	29.56829181648261	32.1386651005002	31.123145733605707
32-33	6.692228557124411	41.96175747540857	21.87212917066551	29.473884796801507
34-35	13.551731538488573	35.85846638038287	21.932276827333844	28.65752525379471
36-37	6.839050255560361	41.61659542715219	22.909407516901354	28.634946800386096
38-39	6.7611136195364505	41.05219281235677	29.878079155288717	22.308614412818066
40-41	6.834289712441409	41.13068388958452	29.650523612088335	22.38450278588574
42-43	7.2476440216355895	34.05055962838923	29.920160327661698	28.781636022313478
44-45	13.964758473358152	34.32684157293371	22.973416017950324	28.734983935757818
46-47	7.444314785301636	40.513121476183564	29.292400426005926	22.750163312508878
48-49	14.4068548035068	33.687124322796386	29.280107425515776	22.625913448181038
50-51	6.886293317060479	33.50243836844158	22.967238480476727	36.64402983402121
52-53	13.667529375517868	33.85982968622612	30.537817736136198	21.934823202119816
54-55	6.802066864467923	33.64769299825521	30.098871625210123	29.451368512066743
56-57	7.233297972188568	33.36469301057735	29.68149095703408	29.720518060200003
58-59	13.952097272595964	33.73485802281689	30.440523348584776	21.87252135600237
60-61	7.273471805843464	34.6935661797843	29.46872051311928	28.564241501252962
62-63	13.785156661837192	35.3836099865147	29.142527350183457	21.68870600146465
64-65	6.7256837357937815	49.29535159403863	22.14242162760458	21.836543042563008
66-67	6.725957740722563	49.214622439929144	22.36955710652689	21.689862712821398
68-69	6.799328322956352	49.08411527237307	22.40322948163849	21.713326923032085
70-71	6.970873969705817	48.075635486691915	22.73227801880987	22.2212125247924
72-73	7.317821023612729	46.26419573208377	23.307337951470043	23.110645292833453
74-75	7.6583768790944635	44.77091476009665	23.65631197636758	23.914396384441304
76-77	7.886717014205412	43.30689975709574	24.175161074629283	24.631222154069565
78-79	8.203136417903352	42.325787070109634	24.384157567222644	25.086918944764374
80-81	8.473717402294314	41.52740285728746	24.613189151565436	25.385690588852793
82-83	8.702450131692196	41.03145571130136	24.315352697095435	25.950741459911008
84-85	9.046191475482848	39.92851188614855	24.75332665616585	26.271969982202748
86-87	9.316007552875675	39.822863968341835	24.718557003825058	26.14257147495743
88-89	9.526573032912355	39.3630044630496	24.626296865333348	26.484125638704704
90-91	9.808773909658717	38.89480389907311	24.55548286187167	26.7409393293965
92-93	10.010259870078041	38.796519855708986	24.47293485852559	26.72028541568738
94-95	10.227423808884206	38.78172120629266	24.247215773976905	26.743639210846233
96-97	10.50061495131228	38.20094156813836	24.39499458974775	26.903448890801616
98-99	10.740861274895291	38.18845771380794	24.123764031816798	26.94691697947997
100-101	11.195843787176063	38.060663374511805	23.72196796859184	27.021524869720288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	6.5
2	6.0
3	11.0
4	27.0
5	48.5
6	69.0
7	104.5
8	163.5
9	287.5
10	550.0
11	1057.5
12	1984.5
13	3538.0
14	5863.0
15	9032.5
16	13337.5
17	19004.5
18	26818.5
19	37708.5
20	52207.0
21	71809.0
22	97793.5
23	130491.5
24	170135.5
25	213850.0
26	257317.0
27	298307.0
28	333435.5
29	359832.5
30	376093.5
31	381483.5
32	380655.0
33	373865.5
34	355386.5
35	333019.5
36	313818.5
37	299833.5
38	289814.5
39	282533.0
40	278786.0
41	277810.0
42	279760.0
43	279967.0
44	273315.0
45	259265.5
46	240003.0
47	215652.5
48	188221.5
49	160539.0
50	135714.0
51	113916.5
52	93669.5
53	76962.5
54	64339.5
55	54491.5
56	46516.5
57	39605.5
58	33700.0
59	28796.5
60	24865.0
61	21432.0
62	18422.0
63	15684.0
64	13272.5
65	11151.5
66	9310.5
67	7873.5
68	6591.5
69	5484.5
70	4545.5
71	3765.0
72	3064.0
73	2524.0
74	2126.0
75	1705.0
76	1394.0
77	1131.5
78	894.5
79	738.5
80	606.0
81	538.0
82	468.0
83	398.0
84	359.5
85	315.0
86	276.0
87	247.0
88	225.0
89	200.0
90	174.0
91	148.5
92	123.0
93	91.5
94	73.0
95	59.0
96	33.0
97	19.5
98	13.0
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001694590639217786
2	6.255200346105921E-4
3	0.012874339621439823
4	0.011407210812989526
5	4.0943129538147853E-4
6	5.686545769187201E-5
7	2.1608873922911365E-4
8	0.0
9	4.549236615349761E-5
10-11	0.0
12-13	0.0
14-15	3.4119274615123206E-5
16-17	5.686545769187201E-6
18-19	3.6393892922798087E-4
20-21	7.961164076862081E-5
22-23	0.0011088764249915042
24-25	5.1178911922684816E-5
26-27	6.255200346105922E-5
28-29	6.710124007640898E-4
30-31	0.001017891692684509
32-33	4.2649093268904006E-4
34-35	0.00276366124382498
36-37	5.117891192268481E-4
38-39	0.0010690706046071938
40-41	6.653258549949026E-4
42-43	0.007836060069939964
44-45	0.00729583822186718
46-47	0.014295976063736626
48-49	0.016792369656409806
50-51	0.02939944162669783
52-53	3.468792919204193E-4
54-55	0.005976559603415749
56-57	8.700415026856418E-4
58-59	8.416087738397058E-4
60-61	0.0012339804319136227
62-63	0.0016718444561410373
64-65	0.0016263520899875395
66-67	0.001472815354219485
68-69	7.449374957635234E-4
70-71	0.0019447986530620228
72-73	3.696254749971681E-4
74-75	2.0471564769073927E-4
76-77	0.0037644932992019274
78-79	0.0033778081868971977
80-81	8.984742315315779E-4
82-83	0.004566296252657323
84-85	3.809985665355425E-4
86-87	7.392509499943362E-5
88-89	0.004293342055736337
90-91	9.269069603775139E-4
92-93	0.00199029101921552
94-95	2.843272884593601E-4
96-97	0.0013420248015281796
98-99	3.696254749971681E-4
100-101	7.278778584559617E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8792684.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.27782123312562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.00223070749828	71.51560939947214
2	3.4440391530482937	5.185195273661096
3	0.6977166837976125	1.5756777538285776
4	0.29561835917430673	0.8901402398061354
5	0.15109475846339868	0.568704210843501
6	0.10696334812073614	0.4831180678997616
7	0.061154312874573215	0.3222494402544729
8	0.05889222182405685	0.35466225171943444
9	0.028568935036831226	0.19355464660710245
>10	0.13127024733628082	1.885502438679026
>50	0.011823916444888902	0.622786198296087
>100	0.008646317059206151	1.296040774899977
>500	9.47536190597396E-4	0.5059002605177337
>1k	8.373495660653949E-4	1.1955185637775292
>5k	7.545934007917823E-5	0.45856138562035714
>10k+	1.2069422476710865E-4	12.946779094117042
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	909595	10.344907197847665	TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT	62834	0.7146168337222173	TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	58988	0.6708759236656292	TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	39971	0.4545938418803633	TruSeq Adapter, Index 6 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	24801	0.28206404324322354	TruSeq Adapter, Index 6 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	18235	0.20738832420225725	TruSeq Adapter, Index 6 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT	13617	0.15486738747804424	TruSeq Adapter, Index 6 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	11942	0.13581745915126714	TruSeq Adapter, Index 6 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT	9361	0.10646350989072279	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAATATCTCGTATGC	8949	0.10177779617691254	TruSeq Adapter, Index 6 (98% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.48266217687341E-4	0.0	1.1373091538374404E-5
2	8.75728048454829E-4	0.0	1.1373091538374404E-5
3	8.984742315315779E-4	0.0	1.1373091538374404E-5
4	9.439665976850754E-4	0.0	1.1373091538374404E-5
5	0.0010690706046071938	0.0	1.1373091538374404E-5
6	0.0012282938861444355	0.0	1.1373091538374404E-5
7	0.0013761440761433028	0.0	1.1373091538374404E-5
8	0.001922052469985274	0.0	1.1373091538374404E-5
9	0.002172260483829511	0.0	1.1373091538374404E-5
10-11	0.0038725376688164845	0.0	1.1373091538374404E-5
12-13	0.006232454163029174	0.0	1.1373091538374404E-5
14-15	0.010053812919922973	0.0	1.1373091538374404E-5
16-17	0.012737862522979333	0.0	1.1373091538374404E-5
18-19	0.014057141141430762	0.0	1.1373091538374404E-5
20-21	0.01560388159064968	0.0	1.1373091538374404E-5
22-23	0.023132868189053538	0.0	1.1373091538374404E-5
24-25	0.038725376688164836	0.0	1.1373091538374404E-5
26-27	0.044775861386580025	0.0	1.1373091538374404E-5
28-29	0.04652163093772049	0.0	2.2746183076748804E-5
30-31	0.04806837138693941	0.0	3.4119274615123206E-5
32-33	0.05004160276884737	2.2746183076748808E-5	3.4119274615123206E-5
34-35	0.05161677594691223	2.2746183076748808E-5	3.4119274615123206E-5
36-37	0.05360138042035856	2.2746183076748808E-5	3.4119274615123206E-5
38-39	0.055745208175342134	2.2746183076748808E-5	3.4119274615123206E-5
40-41	0.05811081121532401	2.2746183076748808E-5	3.4119274615123206E-5
42-43	0.06146018667337527	2.2746183076748808E-5	3.4119274615123206E-5
44-45	0.06594118473949478	2.2746183076748808E-5	3.9805820384310414E-5
46-47	0.07143438795252963	2.2746183076748808E-5	4.5492366153497615E-5
48-49	0.07832079487901533	2.2746183076748808E-5	4.5492366153497615E-5
50-51	0.08704964263471768	2.2746183076748808E-5	5.1178911922684816E-5
52-53	0.09877530001078169	2.2746183076748808E-5	5.686545769187202E-5
54-55	0.11450428560835349	2.2746183076748808E-5	5.686545769187202E-5
56-57	0.13428209179358658	2.2746183076748808E-5	5.686545769187202E-5
58-59	0.16135573620068683	2.2746183076748808E-5	6.255200346105922E-5
60-61	0.19587875556542234	2.2746183076748808E-5	6.823854923024641E-5
62-63	0.2411720926169984	2.2746183076748808E-5	6.823854923024641E-5
64-65	0.3006817940915425	2.8432728845936005E-5	6.823854923024641E-5
66-67	0.37525515530866344	3.9805820384310414E-5	7.961164076862081E-5
68-69	0.46886138521525395	4.5492366153497615E-5	7.961164076862081E-5
70-71	0.5866468077324285	4.5492366153497615E-5	7.961164076862081E-5
72-73	0.7316252921178561	4.5492366153497615E-5	7.961164076862081E-5
74-75	0.906651484347669	4.5492366153497615E-5	7.961164076862081E-5
76-77	1.115882249379143	4.5492366153497615E-5	7.961164076862081E-5
78-79	1.3666247985256834	4.5492366153497615E-5	7.961164076862081E-5
80-81	1.6614380773834245	5.1178911922684816E-5	7.961164076862081E-5
82-83	2.0113710443818977	5.686545769187202E-5	7.961164076862081E-5
84-85	2.412352132750364	5.686545769187202E-5	8.529818653780802E-5
86-87	2.879496181143323	5.686545769187202E-5	9.098473230699523E-5
88-89	3.4149811365903746	5.686545769187202E-5	9.667127807618244E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGA	7615	0.0	84.781654	1
CCGGAAG	8095	0.0	84.73988	3
ACCGGAA	8405	0.0	81.50279	2
GATTGGA	5990	0.0	71.854454	1
GAGCACA	166030	0.0	71.61716	9
AGAGCAC	167375	0.0	71.098404	8
CATCGGA	3085	0.0	70.99014	1
ACGGAAG	4375	0.0	70.904305	3
GAAGAGC	169025	0.0	70.528786	6
AAGAGCA	170530	0.0	69.88128	7
GGAAGAG	169465	0.0	69.12451	5
GAACGGA	4210	0.0	68.94637	1
CGGAAGA	164410	0.0	68.33701	4
TCGGAAG	148450	0.0	68.12914	3
TTGGAAG	6580	0.0	67.06985	3
AATCATA	124360	0.0	66.9346	5
ATCGGAA	151880	0.0	66.4478	2
ATTGGAA	6715	0.0	65.86407	2
TAAATCA	113490	0.0	65.474495	3
TCATAAA	129325	0.0	64.95541	7
>>END_MODULE