##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_TTAGGC_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16000000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.7893868125	31.0	28.0	31.0	16.0	34.0
2	30.224839625	31.0	30.0	33.0	26.0	34.0
3	27.1913035	31.0	25.0	31.0	16.0	34.0
4	31.7766534375	35.0	30.0	37.0	19.0	37.0
5	32.7122475	35.0	32.0	37.0	25.0	37.0
6	34.147507625	35.0	33.0	37.0	30.0	37.0
7	34.7761665625	35.0	35.0	37.0	32.0	37.0
8	34.98924225	35.0	35.0	37.0	32.0	37.0
9	36.6092960625	39.0	35.0	39.0	32.0	39.0
10-11	36.71505928125001	39.0	36.0	39.0	32.5	39.0
12-13	36.76743878125	39.0	36.0	39.0	32.5	39.0
14-15	37.9403200625	40.0	37.0	41.0	33.0	41.0
16-17	37.9073778125	40.0	37.0	41.0	32.5	41.0
18-19	37.909662374999996	39.5	37.0	41.0	33.0	41.0
20-21	37.941397781250004	40.0	37.0	41.0	32.5	41.0
22-23	38.10047565625	40.0	37.0	41.0	33.0	41.0
24-25	37.95349490625	40.0	37.0	41.0	33.0	41.0
26-27	37.6457951875	40.0	37.0	41.0	32.0	41.0
28-29	37.5176529375	40.0	36.5	41.0	31.5	41.0
30-31	37.450817625	39.5	37.0	41.0	31.5	41.0
32-33	37.282735875	39.5	36.0	41.0	31.0	41.0
34-35	36.740535406250004	39.0	35.5	40.0	30.0	41.0
36-37	36.51021875	39.0	35.5	40.0	29.5	41.0
38-39	36.218305	39.0	35.0	40.0	29.0	41.0
40-41	36.93460796875	39.0	36.0	40.0	30.5	41.0
42-43	36.96173521875	39.0	36.0	40.5	30.5	41.0
44-45	36.821559625	39.0	35.5	41.0	30.0	41.0
46-47	36.6738564375	39.0	35.0	40.0	30.0	41.0
48-49	36.642247375	39.0	35.0	40.0	30.0	41.0
50-51	36.4514501875	39.0	35.0	40.0	30.0	41.0
52-53	36.1041874375	38.5	35.0	40.0	29.0	41.0
54-55	36.01258675	38.0	35.0	40.0	29.0	41.0
56-57	35.81950809375	38.0	34.0	40.0	28.0	41.0
58-59	35.4706714375	38.0	34.0	40.0	28.0	41.0
60-61	35.1844019375	37.0	34.0	40.0	28.0	41.0
62-63	34.842953718749996	37.0	33.5	40.0	28.0	41.0
64-65	34.13297421875	36.0	33.0	39.0	26.5	40.0
66-67	33.928317375000006	36.0	33.0	39.0	26.5	40.0
68-69	32.6990273125	35.5	32.5	38.0	23.0	40.0
70-71	29.7505916875	35.0	29.5	37.0	2.0	39.5
72-73	28.96610025	35.0	29.0	36.5	2.0	39.0
74-75	28.61616396875	34.5	29.0	36.0	2.0	39.0
76-77	26.26533421875	31.5	24.5	34.0	2.0	35.5
78-79	27.99434596875	34.0	28.5	35.0	2.0	37.0
80-81	28.09562125	34.0	29.0	35.0	2.0	36.5
82-83	27.95303909375	34.0	29.0	35.0	2.0	36.0
84-85	27.79733471875	34.0	29.0	35.0	2.0	36.0
86-87	27.63840053125	34.0	29.0	35.0	2.0	36.0
88-89	27.45093371875	34.0	28.5	35.0	2.0	35.5
90-91	27.3040483125	34.0	27.5	35.0	2.0	35.0
92-93	27.136187375	34.0	27.0	35.0	2.0	35.0
94-95	26.95385403125	34.0	27.0	35.0	2.0	35.0
96-97	26.765860125000003	34.0	27.0	35.0	2.0	35.0
98-99	26.510800562500002	34.0	25.5	35.0	2.0	35.0
100-101	25.28246584375	32.5	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.476110979366215
1101	2	1.4331895082537898
1101	3	2.863993877002631
1101	4	2.4777119444379885
1101	5	2.8630062055910486
1101	6	1.3921404639512787
1101	7	0.7000836275724112
1101	8	0.49404301029930764
1101	9	0.5654526144022967
1101	10-11	0.5315252003536415
1101	12-13	0.4750690402919844
1101	14-15	0.435252094277196
1101	16-17	0.44639373908251656
1101	18-19	0.34025885539787026
1101	20-21	0.5184661527281378
1101	22-23	0.2468010382078134
1101	24-25	0.35677763527424133
1101	26-27	0.34161091747238004
1101	28-29	0.5710382817497148
1101	30-31	0.5322982954722875
1101	32-33	0.5777776425663603
1101	34-35	0.6757215095889677
1101	36-37	0.7991145937852835
1101	38-39	0.8125645868668983
1101	40-41	0.6122263877664906
1101	42-43	0.4489531337083221
1101	44-45	0.4001447585960136
1101	46-47	0.39458107993497293
1101	48-49	0.3927245454841497
1101	50-51	0.2645143863225812
1101	52-53	0.26820931507366197
1101	54-55	0.11924217836365614
1101	56-57	0.03681207426345878
1101	58-59	-0.04304825064149753
1101	60-61	-0.0732865491333996
1101	62-63	-0.020031419476659096
1101	64-65	-0.6073511321363938
1101	66-67	-0.38007278401586575
1101	68-69	-0.8259945102491315
1101	70-71	0.1428452306217345
1101	72-73	0.4701197596118192
1101	74-75	0.5348131048537113
1101	76-77	-0.36057897311188825
1101	78-79	0.2738738033309076
1101	80-81	0.3979335024217505
1101	82-83	0.3902403840448514
1101	84-85	0.3789453224856061
1101	86-87	0.3997856755513354
1101	88-89	0.3827009103013843
1101	90-91	0.33888275311945293
1101	92-93	0.2930990059079477
1101	94-95	0.30006577491941044
1101	96-97	0.31254718017537897
1101	98-99	0.2790278669188453
1101	100-101	-0.27435768043527986
1102	1	2.6692213735982833
1102	2	1.6570196334893161
1102	3	3.8269019488316793
1102	4	3.013543281996494
1102	5	3.057319609321901
1102	6	1.5898260184634125
1102	7	0.8414031274662364
1102	8	0.5686800200701327
1102	9	0.6348358662255507
1102	10-11	0.6447752361096803
1102	12-13	0.5743627860534986
1102	14-15	0.5040352214616561
1102	16-17	0.5938747095618027
1102	18-19	0.5362331271162191
1102	20-21	0.7236949916764175
1102	22-23	0.5478748352285194
1102	24-25	0.5782261516523164
1102	26-27	0.6227468227923225
1102	28-29	0.7006808711537005
1102	30-31	0.7515758268971311
1102	32-33	0.8526756079994442
1102	34-35	1.0084756746238668
1102	36-37	1.2098842476475014
1102	38-39	1.1070741550499221
1102	40-41	0.9122658326564093
1102	42-43	0.7687544714673535
1102	44-45	0.8565273594419267
1102	46-47	0.6887996289960014
1102	48-49	0.7515790150252002
1102	50-51	0.7120335121792536
1102	52-53	0.8463413175495305
1102	54-55	0.7767049633942449
1102	56-57	0.6618933830117797
1102	58-59	0.58965931678582
1102	60-61	0.5663582152282629
1102	62-63	0.6150798446176822
1102	64-65	0.06037605037716531
1102	66-67	0.3952979855676375
1102	68-69	0.17624847299070723
1102	70-71	0.0895579762723635
1102	72-73	0.2669879292722861
1102	74-75	0.26515428389941675
1102	76-77	0.1176919448954088
1102	78-79	0.21401243136446269
1102	80-81	0.259103086366423
1102	82-83	0.22838977289442397
1102	84-85	0.2660214277035493
1102	86-87	0.2817008239352745
1102	88-89	0.18153154590604004
1102	90-91	0.2241984744093486
1102	92-93	0.16938049296534885
1102	94-95	0.15029561316440976
1102	96-97	0.24953685332411624
1102	98-99	0.22180349826055945
1102	100-101	-0.061087110626569796
1103	1	1.5045534772985611
1103	2	0.7058856256423098
1103	3	1.7805646415858085
1103	4	-0.5982107394734477
1103	5	0.7475811070461305
1103	6	0.41713515677781743
1103	7	0.26785791392399716
1103	8	0.197593445985774
1103	9	0.3103067345290711
1103	10-11	0.34597201895528684
1103	12-13	0.27185330656064366
1103	14-15	0.2902860894852566
1103	16-17	0.25202431428070327
1103	18-19	0.2593696126133196
1103	20-21	0.32424006145711104
1103	22-23	0.31473703883312254
1103	24-25	0.3568037726663249
1103	26-27	0.3360231114184984
1103	28-29	0.45145283996664176
1103	30-31	0.39534984145968366
1103	32-33	0.426801651562009
1103	34-35	0.506375431276247
1103	36-37	0.6545072374437311
1103	38-39	0.6213367475913074
1103	40-41	0.4604041207541343
1103	42-43	0.3170303469157645
1103	44-45	0.30307213704966784
1103	46-47	0.32089276463897676
1103	48-49	0.27668796670058526
1103	50-51	0.19671636633029976
1103	52-53	0.2935387843902646
1103	54-55	0.24050072356366314
1103	56-57	0.21223664928651687
1103	58-59	0.14355267467177413
1103	60-61	0.02296892021085739
1103	62-63	0.1023098623529961
1103	64-65	-0.3151298152459745
1103	66-67	-0.1516826895837795
1103	68-69	-0.36899884019516094
1103	70-71	0.13303496936855908
1103	72-73	0.4653519839548821
1103	74-75	0.45946248797169886
1103	76-77	-0.05941130278656459
1103	78-79	0.32260411705139447
1103	80-81	0.3739736561010716
1103	82-83	0.3315245996756282
1103	84-85	0.29226856519456135
1103	86-87	0.375641480706399
1103	88-89	0.2760533313987672
1103	90-91	0.3535711296716926
1103	92-93	0.2814339193530593
1103	94-95	0.14931563047645824
1103	96-97	0.22186130169561125
1103	98-99	0.16786655462344058
1103	100-101	-0.15084638372685077
1216	1	0.8774864873614305
1216	2	0.25862494042681305
1216	3	0.8317346520890183
1216	4	-0.5583590601959543
1216	5	1.0843742522012931
1216	6	0.4782718859666062
1216	7	0.20676320002727522
1216	8	0.18879830626609362
1216	9	0.12847874421056815
1216	10-11	0.202017982558786
1216	12-13	0.14490429827193907
1216	14-15	0.0928652227053206
1216	16-17	0.11324309283759249
1216	18-19	0.12355986789933837
1216	20-21	0.19522904131439844
1216	22-23	0.08585881500306414
1216	24-25	0.1689125856891991
1216	26-27	0.12003260279449535
1216	28-29	0.2129235144648689
1216	30-31	0.19449122194647117
1216	32-33	0.24916972923719527
1216	34-35	0.36038556891649165
1216	36-37	0.48977234999558306
1216	38-39	0.5706818539864784
1216	40-41	0.2784642263881878
1216	42-43	0.07136292000642896
1216	44-45	0.06565770035987839
1216	46-47	0.11512168210983731
1216	48-49	0.26494616224790235
1216	50-51	0.1544380440001092
1216	52-53	0.16421854655560253
1216	54-55	0.0392137326222084
1216	56-57	0.029038372721011285
1216	58-59	0.031225387248454695
1216	60-61	-0.037423041824915515
1216	62-63	-0.057106126900627885
1216	64-65	-0.38647436737153384
1216	66-67	-0.2588159964719452
1216	68-69	-0.6139965106504164
1216	70-71	0.11240316943369422
1216	72-73	0.4690536319430407
1216	74-75	0.4640948870620889
1216	76-77	-0.004080193162028678
1216	78-79	0.40276003240609626
1216	80-81	0.5216104737463034
1216	82-83	0.498092052723468
1216	84-85	0.5133161496646999
1216	86-87	0.5081925740018107
1216	88-89	0.4710485159017104
1216	90-91	0.448195858510843
1216	92-93	0.435908147049922
1216	94-95	0.3731809500139107
1216	96-97	0.4412860687247928
1216	98-99	0.3895558502561194
1216	100-101	0.12269451892479566
1113	1	2.322594199349627
1113	2	1.209378246534957
1113	3	2.4214854045388847
1113	4	3.234124902528091
1113	5	2.786414916186466
1113	6	1.1492444799639259
1113	7	0.5112261881000748
1113	8	0.2955710793564137
1113	9	0.18671345299251385
1113	10-11	0.11890225954759615
1113	12-13	0.0614015486275008
1113	14-15	0.1104922385027507
1113	16-17	0.04955818049796079
1113	18-19	0.07974891810307128
1113	20-21	0.16417616220885378
1113	22-23	0.014308963635336625
1113	24-25	0.0023295783011789695
1113	26-27	0.04122976466040029
1113	28-29	0.04818232917688903
1113	30-31	0.23128191308297374
1113	32-33	0.3813441930085162
1113	34-35	0.24168607232281403
1113	36-37	0.13360184712441736
1113	38-39	0.21614975160616012
1113	40-41	0.12144768591367949
1113	42-43	-0.04255247953786778
1113	44-45	0.1500211845163122
1113	46-47	0.10643982620567272
1113	48-49	0.0139361040592334
1113	50-51	-0.10171592691919074
1113	52-53	0.05146216117896074
1113	54-55	-0.05954263849173458
1113	56-57	-0.016478667061747387
1113	58-59	-0.08205937768032356
1113	60-61	-0.31935112484234196
1113	62-63	-0.1477465685626811
1113	64-65	-0.6137749841956932
1113	66-67	-0.3616099946560425
1113	68-69	-0.6233084029160736
1113	70-71	0.06190688385849441
1113	72-73	0.4927615004890811
1113	74-75	0.5391314285244349
1113	76-77	0.06871630698271147
1113	78-79	0.4471819148484144
1113	80-81	0.5259535138067548
1113	82-83	0.5908646505591477
1113	84-85	0.6052962894752483
1113	86-87	0.5901861519892364
1113	88-89	0.5622255066291757
1113	90-91	0.542497351836122
1113	92-93	0.5653991088982977
1113	94-95	0.5467008891463934
1113	96-97	0.43308087619355007
1113	98-99	0.33872517645236755
1113	100-101	0.07570949956089379
1112	1	2.0875152319535246
1112	2	1.105535441366019
1112	3	1.5616014793066206
1112	4	2.979092394575755
1112	5	2.2647690113371155
1112	6	0.9860172136797019
1112	7	0.4426857889583218
1112	8	0.294032479490113
1112	9	0.14882472785839695
1112	10-11	0.12903820689549406
1112	12-13	0.04937405389740945
1112	14-15	-0.06315843521565512
1112	16-17	-0.04841100817074562
1112	18-19	0.04669364210823801
1112	20-21	0.1285633665664605
1112	22-23	-0.004869433601690787
1112	24-25	0.05992945266415717
1112	26-27	0.02615492298684785
1112	28-29	-0.08088627586750619
1112	30-31	-0.007125129926230045
1112	32-33	0.12124093266695724
1112	34-35	0.11781847420859037
1112	36-37	0.23544495702297752
1112	38-39	0.23609782039783767
1112	40-41	0.12180941238413823
1112	42-43	-0.02512399966332879
1112	44-45	-0.013294724353798415
1112	46-47	-0.10163145002507434
1112	48-49	-0.1287862777430533
1112	50-51	-0.17055136698443363
1112	52-53	-0.13604311813875114
1112	54-55	-0.1428996892398331
1112	56-57	-0.19322798355570114
1112	58-59	-0.33340155145468486
1112	60-61	-0.3226573097775116
1112	62-63	-0.36074068096814216
1112	64-65	-0.31888515500789083
1112	66-67	-0.34216568414012016
1112	68-69	-0.39213817937319817
1112	70-71	0.18739346308600346
1112	72-73	0.5199462415463607
1112	74-75	0.5968783250385137
1112	76-77	0.08277752930758808
1112	78-79	0.5120898839914716
1112	80-81	0.6031962181649142
1112	82-83	0.5828987410881723
1112	84-85	0.5993407823173627
1112	86-87	0.5610482793524163
1112	88-89	0.5551497042416571
1112	90-91	0.6103613166021589
1112	92-93	0.6201024205332786
1112	94-95	0.5702361478458684
1112	96-97	0.5165830004930285
1112	98-99	0.4843698340531084
1112	100-101	0.3532348836015444
1115	1	1.4307226262210477
1115	2	0.7468815622903762
1115	3	-0.034520622317163685
1115	4	0.07583151769831531
1115	5	1.6579989042263037
1115	6	0.6845585333786133
1115	7	0.3028710226733864
1115	8	0.1494643114527463
1115	9	0.1057886449188814
1115	10-11	0.10523427484027081
1115	12-13	-0.04866789580307085
1115	14-15	-0.12330550812564667
1115	16-17	-0.10773053443406866
1115	18-19	-0.03211574002032336
1115	20-21	-0.004563824369554936
1115	22-23	-0.14338790476173102
1115	24-25	-0.07243508444760494
1115	26-27	-0.1029038441760406
1115	28-29	-0.0986745903198667
1115	30-31	0.018157694290131587
1115	32-33	0.0404137871448782
1115	34-35	-0.015151486177977347
1115	36-37	0.21523603762853583
1115	38-39	0.2513880159689137
1115	40-41	0.07370015131589014
1115	42-43	-0.04983060262598116
1115	44-45	-0.03736056968396895
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1315	72-73	0.5122279611002227
1315	74-75	0.48568811873870743
1315	76-77	1.0474349738073592
1315	78-79	0.5832133037368621
1315	80-81	0.5234480037865268
1315	82-83	0.522445814002765
1315	84-85	0.48792243128870183
1315	86-87	0.5003717009665678
1315	88-89	0.5195448378586853
1315	90-91	0.47754809060857184
1315	92-93	0.4779866271227782
1315	94-95	0.4886823154011246
1315	96-97	0.4699916193996607
1315	98-99	0.5410220696054857
1315	100-101	0.7860338909038198
1214	1	-1.1563493791214725
1214	2	-1.4180653533435965
1214	3	-1.1799290930187212
1214	4	-2.997411155530706
1214	5	-0.01320106178786773
1214	6	-0.032548523210593316
1214	7	0.06927102757985892
1214	8	0.09066867841150383
1214	9	0.1506114286440905
1214	10-11	0.2806580205198941
1214	12-13	0.23907445506577574
1214	14-15	0.29143275840760197
1214	16-17	0.3215859097422822
1214	18-19	0.28589365686055856
1214	20-21	0.2724259827963422
1214	22-23	0.2361141071949291
1214	24-25	0.214937894653211
1214	26-27	0.33883017664434334
1214	28-29	0.33048625707100143
1214	30-31	0.3504512513779403
1214	32-33	0.40398159794098376
1214	34-35	0.27170496391470067
1214	36-37	0.3504862405445621
1214	38-39	0.24745484397359263
1214	40-41	0.19783699437815017
1214	42-43	0.3217863110027608
1214	44-45	0.39974187555861107
1214	46-47	0.33900000091161075
1214	48-49	0.3265197484469553
1214	50-51	0.4244704605540264
1214	52-53	0.4277260942193095
1214	54-55	0.4386459522190478
1214	56-57	0.49043673414669087
1214	58-59	0.5063639846939907
1214	60-61	0.5885825867723682
1214	62-63	0.6029890657893162
1214	64-65	0.8408322711711662
1214	66-67	0.6549060225850525
1214	68-69	0.8914224028301021
1214	70-71	0.037332344940754325
1214	72-73	-0.30064084528981994
1214	74-75	-0.23620952290919206
1214	76-77	0.3695224917446005
1214	78-79	-0.14328029031262446
1214	80-81	-0.22685303731751105
1214	82-83	-0.3487821800648234
1214	84-85	-0.2642136195457354
1214	86-87	-0.2072090606370267
1214	88-89	-0.20120318493166067
1214	90-91	-0.2207580595738854
1214	92-93	-0.20684685650940082
1214	94-95	-0.15181695952743013
1214	96-97	-0.18267575620440724
1214	98-99	-0.1710066499213383
1214	100-101	0.2065314444656572
1316	1	-0.5334110517766781
1316	2	0.06074593871686673
1316	3	-2.6868840221963346
1316	4	-3.812219339278627
1316	5	-0.21630742096100875
1316	6	-0.17882396492724695
1316	7	-0.0304552188569005
1316	8	9.991818227916838E-5
1316	9	0.05124577415998033
1316	10-11	0.14652160273583092
1316	12-13	0.14171192952159117
1316	14-15	0.15174152876867453
1316	16-17	0.2222307950949869
1316	18-19	0.11175557692321547
1316	20-21	0.06521306896834744
1316	22-23	0.10214810290170817
1316	24-25	0.1059001321740567
1316	26-27	0.1628185759176688
1316	28-29	0.1693429374978379
1316	30-31	0.17798429637525714
1316	32-33	0.2070909376384904
1316	34-35	0.023771923434765085
1316	36-37	0.18202809820573407
1316	38-39	0.038824146363417356
1316	40-41	0.1570728696180339
1316	42-43	0.22421083666564812
1316	44-45	0.22627044841709676
1316	46-47	0.23146338792063403
1316	48-49	0.15173697432424404
1316	50-51	0.27860006004400617
1316	52-53	0.3248570738520087
1316	54-55	0.37516009589681687
1316	56-57	0.4491467824361166
1316	58-59	0.4249555440046393
1316	60-61	0.4648113878854545
1316	62-63	0.41151673377463993
1316	64-65	0.7072340936826365
1316	66-67	0.5587442284261357
1316	68-69	0.7297367712566327
1316	70-71	-0.23536062920949163
1316	72-73	-0.5928041437830132
1316	74-75	-0.572785318394903
1316	76-77	0.22774313547399316
1316	78-79	-0.3936088973381153
1316	80-81	-0.48400311411147
1316	82-83	-0.49196706626852915
1316	84-85	-0.42280505397102175
1316	86-87	-0.4135818646530822
1316	88-89	-0.3349488124758331
1316	90-91	-0.3543068958385618
1316	92-93	-0.3437257064528083
1316	94-95	-0.38655703397603247
1316	96-97	-0.3468853730896484
1316	98-99	-0.35591245169769437
1316	100-101	0.12365071166980357
1215	1	-2.4338988307867417
1215	2	-1.090568235064076
1215	3	-3.5180669746874003
1215	4	-3.921322180975121
1215	5	-0.29851482182564126
1215	6	-0.27129076664039076
1215	7	-0.13636654132604775
1215	8	-0.12556171351978662
1215	9	0.007553701371762145
1215	10-11	0.09737935755094895
1215	12-13	-0.05351109583684632
1215	14-15	0.010414438605039322
1215	16-17	0.06227007870165835
1215	18-19	0.06321199064350935
1215	20-21	0.029653697046789773
1215	22-23	-0.03950489105807975
1215	24-25	0.010669469843669788
1215	26-27	0.05900373254386437
1215	28-29	0.08690169017685179
1215	30-31	0.22366001029565297
1215	32-33	0.11801097680300643
1215	34-35	-0.06578885876857044
1215	36-37	0.0056484076590592736
1215	38-39	-0.029836088542161576
1215	40-41	0.013472270520125562
1215	42-43	0.08202518459589214
1215	44-45	0.1569969661832431
1215	46-47	0.14315749658182852
1215	48-49	0.13521768956580615
1215	50-51	0.1540628985913841
1215	52-53	0.207107105330806
1215	54-55	0.24120327125800856
1215	56-57	0.24708397265585091
1215	58-59	0.1949775271598284
1215	60-61	0.3113056648673336
1215	62-63	0.25875527698281786
1215	64-65	0.5365336689321438
1215	66-67	0.4124664904701021
1215	68-69	0.7131981979238091
1215	70-71	0.13520128713117074
1215	72-73	-0.24109467101335014
1215	74-75	-0.24203729508683125
1215	76-77	0.3107255108578464
1215	78-79	-0.12510855368844886
1215	80-81	-0.16504961194100076
1215	82-83	-0.1854399778293896
1215	84-85	-0.1841110267168311
1215	86-87	-0.16650685136422538
1215	88-89	-0.13168635085008162
1215	90-91	-0.12408761225306009
1215	92-93	-0.08946822037985314
1215	94-95	-0.04834703225221659
1215	96-97	-0.035410184203904294
1215	98-99	-0.024023863597474104
1215	100-101	0.36226038609515143
1212	1	-0.41159464743372354
1212	2	0.07582848479566096
1212	3	-1.8847553717699235
1212	4	-1.6767210402784691
1212	5	0.8328921740550754
1212	6	0.2081878486676203
1212	7	0.10182323959489992
1212	8	0.014692212431533846
1212	9	-0.03952864977960502
1212	10-11	-0.06106220177507282
1212	12-13	-0.06240948808643054
1212	14-15	0.02607267008446712
1212	16-17	-0.009257911100760907
1212	18-19	7.432844330779176E-4
1212	20-21	-0.12079144643577422
1212	22-23	-0.05051054690347456
1212	24-25	-0.09542328718031712
1212	26-27	-0.1107033063855738
1212	28-29	-0.08757012311284029
1212	30-31	0.03667117013197441
1212	32-33	-2.9825803580347667
1212	34-35	-0.9617043333881625
1212	36-37	-0.34099792419994657
1212	38-39	-0.2919448249307095
1212	40-41	-0.1427743055074444
1212	42-43	-0.14707513742079215
1212	44-45	-0.16306142479227503
1212	46-47	-0.10846871002029701
1212	48-49	-0.07896829576186803
1212	50-51	0.07403199474089206
1212	52-53	-0.10349276939973606
1212	54-55	-0.025057458876972305
1212	56-57	0.07883441527948065
1212	58-59	4.456796672513974E-4
1212	60-61	0.09532004871225297
1212	62-63	0.14310982460843746
1212	64-65	0.5638627664758644
1212	66-67	0.35930428189149666
1212	68-69	0.782532851147657
1212	70-71	0.3580310867049299
1212	72-73	-0.2093545557506431
1212	74-75	-0.25856894993239266
1212	76-77	0.473858164743735
1212	78-79	-0.11378188001859257
1212	80-81	-0.2050907984482535
1212	82-83	-0.24128476643072005
1212	84-85	-0.24853560481601988
1212	86-87	-0.25110048944446106
1212	88-89	-0.22820004579681452
1212	90-91	-0.15486920383174407
1212	92-93	-0.20741805378349198
1212	94-95	-0.22095975642702115
1212	96-97	-0.18192688271181012
1212	98-99	-0.06159162271522334
1212	100-101	0.32691468423659487
1213	1	-3.2682735415948088
1213	2	-1.507636840917563
1213	3	-2.8841214068054057
1213	4	-3.811588012648869
1213	5	-0.5274009526173238
1213	6	-0.37760126057949606
1213	7	-0.3261245412166005
1213	8	-0.29131507086804476
1213	9	-0.30587632198706416
1213	10-11	-0.0659276902946857
1213	12-13	-0.17534786251414403
1213	14-15	0.004901219077595442
1213	16-17	-3.46640238021223E-4
1213	18-19	0.026554293045371935
1213	20-21	-0.11658536093313643
1213	22-23	-0.21632868044870435
1213	24-25	-0.0983510909687908
1213	26-27	-0.05632149897442673
1213	28-29	-0.14070819273103297
1213	30-31	-0.11653438828397356
1213	32-33	-0.04803587723282021
1213	34-35	-0.4838509239778972
1213	36-37	-0.5168081859743765
1213	38-39	-0.6430703352277973
1213	40-41	-0.3652348513586432
1213	42-43	-0.05365131610074769
1213	44-45	-0.11361439308976884
1213	46-47	-0.08438805141665995
1213	48-49	-0.04388073496667033
1213	50-51	0.026924153218097047
1213	52-53	-0.042000958694103474
1213	54-55	0.1252620161499678
1213	56-57	0.12677734826599618
1213	58-59	0.14077701339736848
1213	60-61	0.156448400695389
1213	62-63	0.04696879496350448
1213	64-65	0.43791198455797087
1213	66-67	0.11024263134392953
1213	68-69	0.6645663640766273
1213	70-71	0.18535814913472493
1213	72-73	-0.43250563828893007
1213	74-75	-0.4870450078268078
1213	76-77	0.1125331986145035
1213	78-79	-0.20264408114159238
1213	80-81	-0.2856123726435058
1213	82-83	-0.2901556579271656
1213	84-85	-0.3159502029091037
1213	86-87	-0.2260789432787682
1213	88-89	-0.3094728933221518
1213	90-91	-0.22963072867560896
1213	92-93	-0.32230781802236663
1213	94-95	-0.2500264512983854
1213	96-97	-0.22197067033572537
1213	98-99	-0.18408757822151145
1213	100-101	0.3945077616290895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2454.0
3	293.0
4	383.0
5	657.0
6	1245.0
7	3070.0
8	6055.0
9	9924.0
10	13622.0
11	17784.0
12	22682.0
13	29041.0
14	33647.0
15	37052.0
16	41515.0
17	49075.0
18	61210.0
19	78704.0
20	102683.0
21	131703.0
22	168157.0
23	220480.0
24	299937.0
25	439484.0
26	639721.0
27	794091.0
28	316687.0
29	329632.0
30	415222.0
31	520127.0
32	658072.0
33	845439.0
34	1116208.0
35	1528781.0
36	2220412.0
37	2953526.0
38	1756527.0
39	134670.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.0806709265675	22.067299815426246	11.321796749584104	41.53023250842215
2	14.873939390151289	36.52426862844981	20.730999653104405	27.870792328294492
3	10.26017686216474	15.437163405184812	39.076380639539366	35.22627909311108
4	13.497386571998963	17.143783890691903	12.227368767421003	57.13146076988813
5	30.876979845465506	20.753149874533772	15.094428891939044	33.27544138806168
6	29.016627817696193	26.529714540535792	17.915811848691202	26.53784579307681
7	7.734838466854808	47.29221216152652	22.895096611887077	22.077852759731595
8	7.841524631143473	48.710805283103966	20.029265021948767	23.4184050638038
9	21.336838833552427	38.35759614734976	21.004651312790706	19.300913706307107
10-11	14.417968749999998	43.0150875	22.817024999999997	19.74991875
12-13	6.921781682611355	45.603987225249206	20.559310659956918	26.914920432182527
14-15	7.686175477894755	42.42366988609526	20.585869024626106	29.30428561138388
16-17	14.568214568214568	36.72289297289297	20.69491756991757	28.01397488897489
18-19	10.2937860282511	31.859017756562146	28.118964041374145	29.728232173812607
20-21	19.23319807707488	31.994264499641535	28.10503613156476	20.667501291718832
22-23	6.7800875	48.40139375	21.260831250000003	23.5576875
24-25	6.8513	34.219340625	28.108690624999998	30.820668750000003
26-27	7.4139946767493345	33.996451124556394	23.669684208710525	34.919869989983745
28-29	14.196688348344175	41.16647683323842	20.511019630509818	24.12581518790759
30-31	6.858837928677371	31.255114453444655	30.96616443538528	30.919883182492697
32-33	7.094565625	41.413484374999996	22.1652	29.32675
34-35	8.126516683384416	33.76594167948284	35.311492321762536	22.796049315370208
36-37	14.238083401765742	40.93629720500153	21.60539583410547	23.220223559127255
38-39	13.997889249736156	33.70525108815639	22.356887169610896	29.939972492496565
40-41	7.119230267065394	40.49574189001596	29.243933367495963	23.141094475422687
42-43	7.466214350651852	33.206656973198456	29.46405883683319	29.8630698393165
44-45	14.340455354094415	33.610513724610854	22.443517481856386	29.60551343943834
46-47	7.702278594878783	40.02055159349384	28.878170258773444	23.398999552853926
48-49	14.702743460047941	33.1680275004561	28.83431210868534	23.294916930810626
50-51	7.267235415580956	33.16038205554857	22.359750409577856	37.21263211929263
52-53	14.121621769428586	33.11149563257027	29.908895081371917	22.857987516629233
54-55	7.0283627967212645	33.1694092435849	29.788181921676877	30.01404603801696
56-57	7.486569234337997	33.04015696823088	29.25398308885876	30.21929070857236
58-59	14.273537473363223	33.16898505715481	29.904659166441927	22.652818303040036
60-61	7.499225621708892	33.891056536990284	29.230652355272507	29.379065486028317
62-63	14.189493350081737	34.59560003796266	28.890838827548876	22.32406778440673
64-65	7.1182540111859165	48.543866081748696	21.756343910838417	22.581535996226965
66-67	7.081900463716306	48.63379849550487	21.905003189756677	22.379297851022145
68-69	7.1898873609167895	48.44330900107967	22.018764024181646	22.348039613821893
70-71	7.4307562028463465	47.0027349455339	22.39873142127355	23.167777430346202
72-73	7.868475	45.059	23.08640625	23.98611875
74-75	8.330525502757924	43.610396366926615	23.39450997715798	24.664568153157486
76-77	8.64969931854746	42.08188737031022	23.851573293500607	25.416840017641718
78-79	8.963990686983703	41.471956950924664	24.035029561301734	25.529022800789903
80-81	9.457623479789302	40.670434149034364	24.161729964735127	25.71021240644121
82-83	9.708335701870508	40.111937249239105	24.004032331024654	26.17569471786574
84-85	10.045134258551936	39.617681464303864	24.13090794363138	26.206276333512825
86-87	10.375309961421815	39.44326788010606	24.076646994760928	26.104775163711192
88-89	10.882747426717142	38.82945239340775	23.79971799982375	26.488082180051364
90-91	11.138162848067589	38.85143558910736	23.711541365985667	26.298860196839378
92-93	11.549653134093171	38.48777189631466	23.59059416735776	26.3719808022344
94-95	11.896964278404011	38.48647045606605	23.200906125849492	26.415659139680443
96-97	12.215227029924714	38.0735124160618	23.18728535169899	26.523975202314492
98-99	12.634024806223934	37.78667424917781	22.943477448232503	26.635823496365763
100-101	13.228078708404265	37.34252290972169	22.585993996075093	26.84340438579896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	1.5
4	4.0
5	10.5
6	14.5
7	16.5
8	30.0
9	51.5
10	95.5
11	159.0
12	290.0
13	593.5
14	1202.5
15	2132.5
16	3471.0
17	5518.5
18	8528.5
19	12923.5
20	19772.5
21	30681.0
22	47774.5
23	75068.5
24	116021.0
25	172229.5
26	245669.5
27	335571.0
28	435315.0
29	533899.5
30	621400.5
31	693280.5
32	746921.0
33	779630.5
34	789000.0
35	782278.5
36	764430.0
37	739908.5
38	718484.0
39	701784.0
40	690375.5
41	681443.5
42	668662.0
43	646777.0
44	614006.0
45	568797.5
46	510640.0
47	445436.5
48	375954.0
49	308518.5
50	251883.5
51	202473.0
52	153538.0
53	111167.5
54	81867.5
55	61096.5
56	45985.5
57	35346.0
58	27662.5
59	22112.0
60	18077.5
61	14977.5
62	12640.0
63	10866.0
64	9207.0
65	7726.5
66	6604.5
67	5647.0
68	4800.5
69	4029.5
70	3368.0
71	2859.5
72	2427.5
73	1933.5
74	1479.5
75	1192.5
76	949.5
77	727.0
78	547.5
79	414.5
80	311.0
81	238.0
82	194.5
83	154.0
84	125.0
85	99.0
86	70.0
87	71.0
88	78.0
89	68.5
90	46.0
91	30.5
92	29.0
93	29.5
94	26.5
95	19.0
96	13.5
97	8.0
98	4.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01625625
2	0.0059375
3	0.0026375
4	0.01518125
5	4.8125E-4
6	3.125E-5
7	1.2499999999999999E-5
8	7.500000000000001E-5
9	6.2499999999999995E-6
10-11	0.0
12-13	6.2499999999999995E-6
14-15	4.6875E-5
16-17	9.999999999999999E-5
18-19	3.5E-4
20-21	6.2499999999999995E-6
22-23	0.0
24-25	0.0
26-27	1.2499999999999999E-5
28-29	4.9999999999999996E-5
30-31	6.2499999999999995E-6
32-33	0.0
34-35	2.4375E-4
36-37	1.6874999999999998E-4
38-39	1.2499999999999999E-5
40-41	3.8124999999999997E-4
42-43	0.0035406249999999995
44-45	0.005703125
46-47	0.0047125
48-49	0.018021875
50-51	0.01519375
52-53	0.00196875
54-55	7.15625E-4
56-57	4.6562499999999995E-4
58-59	2.1874999999999998E-4
60-61	4.25E-4
62-63	4.1562500000000003E-4
64-65	9.34375E-4
66-67	0.0021687499999999997
68-69	0.001128125
70-71	2.9374999999999996E-4
72-73	0.0
74-75	6.4375E-4
76-77	0.002665625
78-79	1.75E-4
80-81	0.002296875
82-83	0.002009375
84-85	4.34375E-4
86-87	1.5625E-4
88-89	6.25E-5
90-91	3.1249999999999997E-6
92-93	8.125E-5
94-95	9.375E-5
96-97	0.00175625
98-99	1.4687499999999998E-4
100-101	8.312500000000001E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.75623679918676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09828039474147	50.48738801268131
2	10.792677407007654	13.114449284747797
3	2.6475223154357104	4.825604781832297
4	1.1899413033238826	2.8918542240751495
5	0.5906684876625025	1.7943397253120263
6	0.3879251345577255	1.4141322801327334
7	0.23793956843237205	1.0119418934501447
8	0.20601817356150479	1.0013511150270995
9	0.1632694592432345	0.8927674135071414
>10	0.6507027572184648	6.53188526995057
>50	0.022437864996938818	0.9311525758612792
>100	0.010832296501425075	1.2173616074918825
>500	0.0010153619810358838	0.43957987843444846
>1k	5.809264056964353E-4	0.6637055381048533
>5k	8.474150584572328E-5	0.3788766406822875
>10k+	1.0380742451259559E-4	12.403609758708928
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	1702978	10.643612500000001	TruSeq Adapter, Index 3 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT	96297	0.60185625	TruSeq Adapter, Index 3 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	44773	0.27983125	TruSeq Adapter, Index 3 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	34738	0.21711249999999999	TruSeq Adapter, Index 3 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	24832	0.1552	TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	18750	0.1171875	TruSeq Adapter, Index 3 (98% over 50bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.25E-4	0.0	1.25E-5
2	6.625E-4	0.0	1.25E-5
3	7.5E-4	0.0	1.25E-5
4	8.25E-4	0.0	1.875E-5
5	9.75E-4	0.0	1.875E-5
6	0.00118125	0.0	1.875E-5
7	0.00146875	0.0	1.875E-5
8	0.00206875	0.0	1.875E-5
9	0.0022375	0.0	3.75E-5
10-11	0.003896875	0.0	3.75E-5
12-13	0.007096875000000001	0.0	3.75E-5
14-15	0.01299375	0.0	3.75E-5
16-17	0.016015625	0.0	3.75E-5
18-19	0.01733125	0.0	3.75E-5
20-21	0.018684375	0.0	3.75E-5
22-23	0.024234375	0.0	3.75E-5
24-25	0.035146874999999994	1.875E-5	3.75E-5
26-27	0.039178124999999994	1.875E-5	3.75E-5
28-29	0.040656250000000005	2.4999999999999998E-5	3.75E-5
30-31	0.042228125	3.125E-5	3.75E-5
32-33	0.044375	3.125E-5	3.75E-5
34-35	0.046415625	3.125E-5	3.75E-5
36-37	0.048875	3.125E-5	3.75E-5
38-39	0.05179375	3.75E-5	3.75E-5
40-41	0.055703125	3.75E-5	3.75E-5
42-43	0.060415625	3.75E-5	4.375E-5
44-45	0.066365625	3.75E-5	4.375E-5
46-47	0.073975	3.75E-5	4.375E-5
48-49	0.083878125	3.75E-5	4.6875E-5
50-51	0.09720000000000001	5.3125000000000004E-5	5.0E-5
52-53	0.11552812500000001	5.625E-5	5.625E-5
54-55	0.13973750000000001	5.625E-5	7.1875E-5
56-57	0.17333125	5.625E-5	7.5E-5
58-59	0.219040625	5.625E-5	7.5E-5
60-61	0.2822875	5.625E-5	7.5E-5
62-63	0.36891562499999997	5.625E-5	7.5E-5
64-65	0.482271875	5.625E-5	7.812499999999999E-5
66-67	0.6307906249999999	5.625E-5	8.125E-5
68-69	0.823996875	5.625E-5	8.125E-5
70-71	1.072715625	5.625E-5	8.75E-5
72-73	1.3864625	5.625E-5	8.75E-5
74-75	1.7772937500000001	5.625E-5	8.75E-5
76-77	2.256928125	5.625E-5	8.75E-5
78-79	2.8343437500000004	5.625E-5	9.375E-5
80-81	3.51678125	5.9375E-5	9.375E-5
82-83	4.31533125	6.25E-5	9.375E-5
84-85	5.2361249999999995	6.25E-5	9.375E-5
86-87	6.290196875	6.25E-5	9.375E-5
88-89	7.47309375	6.25E-5	9.375E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGAAG	12640	0.0	84.788666	3
GACCGGA	12330	0.0	84.42899	1
ACCGGAA	13520	0.0	78.99125	2
TCCGAAG	5340	0.0	70.724785	3
CGAAGAG	6265	0.0	70.65831	5
GAGCACA	348135	0.0	62.328823	9
GCGGAAG	4935	0.0	62.185787	3
ACGGAAG	6710	0.0	61.09899	3
AGAGCAC	355860	0.0	60.97846	8
CATCGGA	3770	0.0	60.115894	1
CCGAAGA	7425	0.0	59.500607	4
AAGAGCA	365340	0.0	59.454666	7
GAAGAGC	370655	0.0	58.62921	6
GAGCGGA	5030	0.0	58.47025	1
AGCGGAA	5210	0.0	58.26698	2
AATCATA	137145	0.0	56.486202	5
GGAAGAG	378115	0.0	56.18295	5
GATTGGA	8630	0.0	55.936485	1
GAACGGA	7135	0.0	55.737026	1
TTGGAAG	8755	0.0	55.18367	3
>>END_MODULE