##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2112_lane1_TTAGGC_L001_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9752634
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.905978220858078	31.0	26.0	31.0	16.0	34.0
2	29.72863084988117	31.0	30.0	33.0	25.0	34.0
3	26.345921829938455	30.0	23.0	31.0	10.0	34.0
4	30.61186454859272	35.0	28.0	35.0	19.0	37.0
5	32.5976551565454	35.0	32.0	37.0	25.0	37.0
6	34.1392607371506	35.0	33.0	37.0	30.0	37.0
7	34.840337697487676	35.0	35.0	37.0	32.0	37.0
8	35.06695729584438	36.0	35.0	37.0	32.0	37.0
9	36.78914824446401	39.0	37.0	39.0	32.0	39.0
10-11	36.97953998888916	39.0	37.0	39.0	33.0	39.0
12-13	36.98808352697333	39.0	37.0	39.0	33.0	39.0
14-15	38.21394081844966	40.0	37.0	41.0	33.0	41.0
16-17	38.17681474563692	40.0	37.0	41.0	33.0	41.0
18-19	38.20110582433423	40.0	37.5	41.0	33.5	41.0
20-21	38.21018726838309	40.0	37.5	41.0	33.0	41.0
22-23	38.33664074751498	40.0	38.0	41.0	34.0	41.0
24-25	38.17992662290003	40.0	37.5	41.0	33.0	41.0
26-27	37.91842275635484	40.0	37.0	41.0	33.0	41.0
28-29	37.86084010740073	40.0	37.0	41.0	32.5	41.0
30-31	37.82265339804611	40.0	37.0	41.0	33.0	41.0
32-33	37.731796763828115	40.0	37.0	41.0	32.5	41.0
34-35	37.100144586580406	40.0	36.0	41.0	30.5	41.0
36-37	36.94998376848757	40.0	36.0	41.0	30.0	41.0
38-39	36.57823409552742	39.0	36.0	41.0	30.0	41.0
40-41	37.262119802711766	40.0	36.5	41.0	30.5	41.0
42-43	37.315273494319584	40.0	37.0	41.0	31.0	41.0
44-45	37.209032349619605	40.0	36.5	41.0	31.0	41.0
46-47	37.0157093458034	39.0	36.0	41.0	31.0	41.0
48-49	36.9887149974048	39.5	36.0	41.0	31.0	41.0
50-51	36.882909273535745	39.0	36.0	41.0	31.0	41.0
52-53	36.53407366666277	39.0	35.0	40.5	30.0	41.0
54-55	36.42666786224111	39.0	35.0	40.0	30.0	41.0
56-57	36.23766984385962	39.0	35.0	40.0	30.0	41.0
58-59	35.8965662507175	38.0	34.5	40.0	29.0	41.0
60-61	35.64309503463372	38.0	34.0	40.0	29.0	41.0
62-63	35.26768640143781	37.0	34.0	40.0	28.0	41.0
64-65	34.692976123168364	36.0	34.0	39.0	28.0	41.0
66-67	34.468257549704006	36.0	34.0	39.0	28.0	40.0
68-69	33.39591919475292	35.5	33.5	38.0	26.0	40.0
70-71	30.151645340120425	35.0	30.5	37.0	2.0	39.5
72-73	28.93779962418358	35.0	29.0	37.0	2.0	39.0
74-75	28.581121366802037	35.0	29.0	36.0	2.0	39.0
76-77	26.811380033332533	32.0	25.5	34.5	2.0	36.5
78-79	28.0344150616131	34.0	28.5	35.0	2.0	37.0
80-81	28.05656492389646	34.0	29.0	35.0	2.0	37.0
82-83	27.893937268639426	34.0	29.0	35.0	2.0	36.0
84-85	27.71650161382043	34.0	29.0	35.0	2.0	36.0
86-87	27.562643025463686	34.0	29.0	35.0	2.0	36.0
88-89	27.3947569959049	34.0	28.5	35.0	2.0	36.0
90-91	27.256859223877363	34.0	27.5	35.0	2.0	35.0
92-93	27.12207819959203	34.0	27.0	35.0	2.0	35.0
94-95	26.970924777860013	34.0	27.0	35.0	2.0	35.0
96-97	26.77728893548143	34.0	27.0	35.0	2.0	35.0
98-99	26.558010635895904	34.0	25.5	35.0	2.0	35.0
100-101	25.55222835184833	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	-2.3630779784138483
2306	2	-0.9629716335785652
2306	3	-2.411056843191435
2306	4	-1.2868407989345911
2306	5	0.2763377578623363
2306	6	-0.14978462768664258
2306	7	-0.42869949632864746
2306	8	-0.24654978078773837
2306	9	-0.336267231906767
2306	10-11	-0.3810936826194222
2306	12-13	-0.30588605153255344
2306	14-15	-0.35054348848489525
2306	16-17	-0.27216171458144345
2306	18-19	-0.36649455830876576
2306	20-21	-0.47316066250336775
2306	22-23	-0.47216904586645825
2306	24-25	-0.3931178112465048
2306	26-27	-0.5270234039531374
2306	28-29	-0.5087583098407151
2306	30-31	-0.5074883570851583
2306	32-33	-0.6033309073688997
2306	34-35	-0.7455220388764587
2306	36-37	-0.8006303569876891
2306	38-39	-0.9212650197086205
2306	40-41	-0.7012273615038893
2306	42-43	-0.5040163382848348
2306	44-45	-0.5593575507332602
2306	46-47	-0.4122631388521185
2306	48-49	-0.49646478430426555
2306	50-51	-0.5402958767025083
2306	52-53	-0.41718667819083777
2306	54-55	-0.4166373580512186
2306	56-57	-0.41866182154087994
2306	58-59	-0.42423576407927044
2306	60-61	-0.27510096822326346
2306	62-63	-0.32222903052518603
2306	64-65	-0.20788033947839324
2306	66-67	-0.3444981960786606
2306	68-69	-0.1661054020363366
2306	70-71	-0.0063969778574808345
2306	72-73	-0.349271084004787
2306	74-75	-0.43510122189352174
2306	76-77	-0.40271341460885424
2306	78-79	-0.4252897732739669
2306	80-81	-0.4160136153183558
2306	82-83	-0.3634829938212185
2306	84-85	-0.3238201599706194
2306	86-87	-0.30235382815745737
2306	88-89	-0.29986980419309717
2306	90-91	-0.26924725349600465
2306	92-93	-0.39796720485111337
2306	94-95	-0.3538032743849371
2306	96-97	-0.26968072211341365
2306	98-99	-0.25095225095592255
2306	100-101	-0.08771824422371566
2307	1	-1.913255954239169
2307	2	-2.3343298572495286
2307	3	-3.5817792999387343
2307	4	0.489353791003758
2307	5	0.2824556913626495
2307	6	-0.29392468020442664
2307	7	-0.18400768058346983
2307	8	-0.25520547920559267
2307	9	-0.5975017505307179
2307	10-11	-0.6028581759390761
2307	12-13	-0.643196852553892
2307	14-15	-0.5819914819106771
2307	16-17	-0.7072484457851331
2307	18-19	-0.5678159715401776
2307	20-21	-0.7137870479049724
2307	22-23	-0.6557627395230838
2307	24-25	-0.681991890812192
2307	26-27	-0.5760383039978336
2307	28-29	-0.7453749258132945
2307	30-31	-0.7670773581416412
2307	32-33	-0.7819473915091564
2307	34-35	-0.9629677654343567
2307	36-37	-1.0805035460283676
2307	38-39	-1.16999485060024
2307	40-41	-0.9354225875711464
2307	42-43	-0.7933999559088605
2307	44-45	-0.9322574068751805
2307	46-47	-0.7741300256888834
2307	48-49	-0.8819923889519785
2307	50-51	-0.8173298423984576
2307	52-53	-0.8875612753866733
2307	54-55	-0.7691963151022492
2307	56-57	-0.8170005467121015
2307	58-59	-0.8031636363997237
2307	60-61	-0.49607238765584327
2307	62-63	-0.4930016381447473
2307	64-65	-0.4372359815595317
2307	66-67	-0.2265655445299899
2307	68-69	-0.183771638296939
2307	70-71	0.5505661027135957
2307	72-73	0.0015484125419895634
2307	74-75	-0.1746250624306498
2307	76-77	-0.033593870030561135
2307	78-79	-0.15532032985202093
2307	80-81	-0.141553410077087
2307	82-83	-0.15755631936206171
2307	84-85	-0.1705656677280203
2307	86-87	-0.11877061087923835
2307	88-89	-0.10775253709408261
2307	90-91	-0.126341644578023
2307	92-93	-0.15209415280979144
2307	94-95	-0.13985532704646175
2307	96-97	-0.16485420386067418
2307	98-99	-0.08355070253040608
2307	100-101	0.17633730365832534
2201	1	-3.427463114891296
2201	2	-1.9377861976895616
2201	3	-2.2626131067793125
2201	4	0.2190543557999831
2201	5	-5.160274426526776
2201	6	-2.3658146757747254
2201	7	-1.1833415131038194
2201	8	-0.7109337438624905
2201	9	-0.841429367079293
2201	10-11	-0.7284562866020323
2201	12-13	-0.8443684663585671
2201	14-15	-0.7638831996870294
2201	16-17	-0.8972272032062207
2201	18-19	-0.7137430055143668
2201	20-21	-0.9831283510745337
2201	22-23	-0.9493198037264463
2201	24-25	-2.7012411505628364
2201	26-27	-2.312184349397363
2201	28-29	-1.2372414052402974
2201	30-31	-1.0762635501685764
2201	32-33	-1.0811591216224414
2201	34-35	-1.1029410304705038
2201	36-37	-1.1884225712465337
2201	38-39	-1.1864122404227047
2201	40-41	-1.1411034420164654
2201	42-43	-0.7731761574665441
2201	44-45	-0.7986729386239659
2201	46-47	-0.865212756919739
2201	48-49	-0.9620213950152703
2201	50-51	-0.9887339297641873
2201	52-53	-0.8860859254479081
2201	54-55	-0.7049255594259023
2201	56-57	-0.7474913819423961
2201	58-59	-0.7393553690160104
2201	60-61	-0.6906551045851117
2201	62-63	-0.6857252175276258
2201	64-65	-0.6813015491096408
2201	66-67	-0.5884171947641477
2201	68-69	-0.44535085716709943
2201	70-71	1.0727922508771712
2201	72-73	0.28413882320762696
2201	74-75	0.045932708116783516
2201	76-77	0.12442144619548401
2201	78-79	0.13617744741563342
2201	80-81	0.14535439672422967
2201	82-83	0.1754583439007078
2201	84-85	0.050402820417129846
2201	86-87	0.09548997296527517
2201	88-89	0.17701254545294987
2201	90-91	0.17393522072459078
2201	92-93	0.16832047655311655
2201	94-95	0.1428703330437031
2201	96-97	0.14445133180964476
2201	98-99	0.18916174970751243
2201	100-101	0.47367210328408405
2202	1	3.823865691342011
2202	2	2.3767825304872296
2202	3	4.616133164616919
2202	4	4.099908254982797
2202	5	3.112720935051165
2202	6	1.6400069635352281
2202	7	0.8930573645341724
2202	8	0.6476851516571358
2202	9	0.7651908454672451
2202	10-11	0.6258273396939558
2202	12-13	0.6170727244346921
2202	14-15	0.6040743361583907
2202	16-17	0.6498039370885138
2202	18-19	0.5634573526482782
2202	20-21	0.7400502027723164
2202	22-23	0.6923222244147809
2202	24-25	0.7240997813665402
2202	26-27	0.6666303984330071
2202	28-29	0.6977879371816869
2202	30-31	0.7044999871614976
2202	32-33	0.7544087088066647
2202	34-35	0.9072130315703504
2202	36-37	0.2243132342937173
2202	38-39	0.4932825582093514
2202	40-41	0.641113775649508
2202	42-43	0.5758168360690021
2202	44-45	0.6796470439446409
2202	46-47	0.6443432884850182
2202	48-49	0.6390390800521146
2202	50-51	0.5579515544848945
2202	52-53	0.6622145582371743
2202	54-55	0.46882329699768377
2202	56-57	0.33320098762506234
2202	58-59	0.18333768223972413
2202	60-61	0.008609875096489361
2202	62-63	0.0016281328592029354
2202	64-65	-0.40830884800880796
2202	66-67	-0.04845343949997982
2202	68-69	-0.444655749449673
2202	70-71	-0.162470905923918
2202	72-73	0.31087333155658214
2202	74-75	0.27132939754864793
2202	76-77	-0.3177987185023845
2202	78-79	0.12931128319743124
2202	80-81	0.1906486683132229
2202	82-83	0.20035615939402618
2202	84-85	0.22577370585249312
2202	86-87	0.20076826426967642
2202	88-89	0.20037501340007324
2202	90-91	0.20132844124637472
2202	92-93	0.17732976124618816
2202	94-95	0.19189496012389284
2202	96-97	0.1743028576781036
2202	98-99	0.1369378734988942
2202	100-101	-0.40018615845508876
2304	1	3.385295666746316
2304	2	2.154075423489516
2304	3	4.7963043933332585
2304	4	3.251406307500826
2304	5	2.7422112744408977
2304	6	1.4590239599448722
2304	7	0.7612720283269638
2304	8	0.4881406463407103
2304	9	0.6608948217499204
2304	10-11	0.5430061436679381
2304	12-13	0.5076544948357906
2304	14-15	0.5095964168707638
2304	16-17	0.6050426336749268
2304	18-19	0.514655308454131
2304	20-21	0.727939189050403
2304	22-23	0.6185729971817651
2304	24-25	0.6552292499078973
2304	26-27	0.6790576611176036
2304	28-29	0.7620577407942548
2304	30-31	0.6914370092095865
2304	32-33	0.7168887631442473
2304	34-35	0.9156101536824934
2304	36-37	0.8358849834012005
2304	38-39	0.8044738803209981
2304	40-41	0.7065117656806024
2304	42-43	0.6484869530971622
2304	44-45	0.7017833643789828
2304	46-47	0.5886490221225387
2304	48-49	0.6379895025821511
2304	50-51	0.6714206690812645
2304	52-53	0.7242396682306662
2304	54-55	0.5819193250407011
2304	56-57	0.5091961204738666
2304	58-59	0.3736402527741305
2304	60-61	0.2862414997148548
2304	62-63	0.37730095853998336
2304	64-65	-0.07658589000312332
2304	66-67	0.22130120736267855
2304	68-69	-0.11996762137297168
2304	70-71	-0.16076344490046068
2304	72-73	0.1511145008411212
2304	74-75	0.19337173654160722
2304	76-77	-0.16032812793229212
2304	78-79	0.1049137435903944
2304	80-81	0.1534348769332503
2304	82-83	0.1668578886175176
2304	84-85	0.11671802356701377
2304	86-87	0.12499793153518368
2304	88-89	0.08927376276388443
2304	90-91	0.13446170985088912
2304	92-93	0.10047743120924224
2304	94-95	0.06426059073053736
2304	96-97	0.1337230489918788
2304	98-99	0.1253676215414039
2304	100-101	-0.23962595746747084
2203	1	2.79082034702083
2203	2	1.78592971752175
2203	3	3.556958005478659
2203	4	1.1428125784776064
2203	5	1.377328726171811
2203	6	0.7832352505026066
2203	7	0.46641359751449585
2203	8	0.34984997404184526
2203	9	0.5060355803217291
2203	10-11	0.4436063137447803
2203	12-13	0.47016563498951314
2203	14-15	0.4493877995205011
2203	16-17	0.5349264167341943
2203	18-19	0.5187276481395244
2203	20-21	0.6480864150535055
2203	22-23	0.5404329220482893
2203	24-25	0.6316563588693995
2203	26-27	0.5962712507346879
2203	28-29	0.6270796630302158
2203	30-31	0.6166704658130726
2203	32-33	0.5880250807148215
2203	34-35	0.8003124113291022
2203	36-37	0.8576858715735014
2203	38-39	0.8637180410514631
2203	40-41	0.6823594134821107
2203	42-43	0.5764405086074547
2203	44-45	0.5874449700537312
2203	46-47	0.5105679530647791
2203	48-49	0.5586316934361193
2203	50-51	0.5031686613380444
2203	52-53	0.49928566950400466
2203	54-55	0.4025352044243533
2203	56-57	0.35938119340386265
2203	58-59	0.2901913389840729
2203	60-61	0.1022598524338818
2203	62-63	0.20527505208785612
2203	64-65	-0.15062369045084978
2203	66-67	0.06654041272621214
2203	68-69	-0.2263016695051263
2203	70-71	0.009097736814283053
2203	72-73	0.44025145066755655
2203	74-75	0.46686461161132087
2203	76-77	0.04200142644837257
2203	78-79	0.32051478573597336
2203	80-81	0.3949401313516212
2203	82-83	0.41415142257384474
2203	84-85	0.3734839530025411
2203	86-87	0.3287804660635416
2203	88-89	0.19419507567686622
2203	90-91	0.23831073941400405
2203	92-93	0.27468672190770604
2203	94-95	0.2607330691948171
2203	96-97	0.27368362728221385
2203	98-99	0.301768858236791
2203	100-101	-9.048969308587118E-4
2305	1	2.6609687865202822
2305	2	1.492885545049642
2305	3	3.051585180067054
2305	4	0.933409279698683
2305	5	1.5664228916478535
2305	6	0.8779524012848867
2305	7	0.5163014682556124
2305	8	0.39063477473293773
2305	9	0.48277960266420195
2305	10-11	0.43663280663547965
2305	12-13	0.42292558387880774
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2208	82-83	-0.2680039655804798
2208	84-85	-0.264094285257233
2208	86-87	-0.2368825486009385
2208	88-89	-0.22117893256764276
2208	90-91	-0.1864290926785479
2208	92-93	-0.211080619669044
2208	94-95	-0.18722038326621515
2208	96-97	-0.14286600526855509
2208	98-99	-0.14450270389588482
2208	100-101	-0.01484557952100829
2215	1	-1.0447840897395722
2215	2	-0.2470935002800445
2215	3	-0.06216468352805293
2215	4	-0.221709672138239
2215	5	0.7550373567854152
2215	6	0.2332140972958925
2215	7	0.016059043273948248
2215	8	-0.0904525703941843
2215	9	-0.1632918185442307
2215	10-11	-0.20183559790045535
2215	12-13	-0.17135350356534218
2215	14-15	-0.26628737554134574
2215	16-17	-0.2032595633099703
2215	18-19	-0.13822632323752515
2215	20-21	-0.24463059282170008
2215	22-23	-0.23700335889515856
2215	24-25	-0.19331180510878454
2215	26-27	-0.2223461717010764
2215	28-29	-0.21711918264158214
2215	30-31	-0.2330301102424457
2215	32-33	-0.2803148111462619
2215	34-35	-0.5286379403043213
2215	36-37	-0.538902301864546
2215	38-39	-0.6228247532294873
2215	40-41	-0.34502850792684825
2215	42-43	-0.3852766905721765
2215	44-45	-0.25877953508560836
2215	46-47	-0.36286365747329086
2215	48-49	-0.3264829967072842
2215	50-51	-0.30055726061378607
2215	52-53	-0.39809698437147034
2215	54-55	-0.33527322369181434
2215	56-57	-0.26543173997740155
2215	58-59	-0.18276480282995777
2215	60-61	-0.1367006127386574
2215	62-63	-0.18703926273359883
2215	64-65	-0.0017949482766042024
2215	66-67	-0.07231167545308637
2215	68-69	0.05251083113913779
2215	70-71	0.17742689702183156
2215	72-73	-0.5413560821841337
2215	74-75	-0.6273197200360627
2215	76-77	-0.39978701790928284
2215	78-79	-0.5216396486427257
2215	80-81	-0.5480034498805857
2215	82-83	-0.5244236660894899
2215	84-85	-0.4795992916463554
2215	86-87	-0.5318840234804938
2215	88-89	-0.4577614221646229
2215	90-91	-0.443481892531306
2215	92-93	-0.445574012417719
2215	94-95	-0.45242569954288925
2215	96-97	-0.4601680198338549
2215	98-99	-0.44274033008898783
2215	100-101	-0.2938263462909987
2214	1	-5.1865627510659245
2214	2	-2.701536214084836
2214	3	-4.616364430663033
2214	4	-3.516137132783932
2214	5	-0.920835810387306
2214	6	-0.7199420611207472
2214	7	-0.5327945783566861
2214	8	-0.45349356952424813
2214	9	-0.5493816541653231
2214	10-11	-0.516010789105195
2214	12-13	-0.41364249491677896
2214	14-15	-0.4555941997963231
2214	16-17	-0.5083843522579627
2214	18-19	-0.5166434945739198
2214	20-21	-0.60816147507294
2214	22-23	-0.7085702421443898
2214	24-25	-0.527878286345917
2214	26-27	-0.5714616140625708
2214	28-29	-0.5926765512374743
2214	30-31	-0.5887467953579275
2214	32-33	-0.5964511623084476
2214	34-35	-0.8676482062391102
2214	36-37	-0.8217458262961372
2214	38-39	-0.8946226780921833
2214	40-41	-0.8303803117836637
2214	42-43	-0.6717148170790708
2214	44-45	-0.7000045584944843
2214	46-47	-0.6384269531774365
2214	48-49	-0.7222476640117463
2214	50-51	-0.6656968957210196
2214	52-53	-0.701181924528214
2214	54-55	-0.6497675285932303
2214	56-57	-0.5420823579896279
2214	58-59	-0.6243450024140529
2214	60-61	-0.6139745385907105
2214	62-63	-0.6153398792387037
2214	64-65	-0.30854249674830925
2214	66-67	-0.33681303629154513
2214	68-69	-0.12481694114968178
2214	70-71	0.4517027162883167
2214	72-73	-0.34186854754299745
2214	74-75	-0.4617002045764096
2214	76-77	-0.09453020616030372
2214	78-79	-0.37278282262887075
2214	80-81	-0.4129549091103044
2214	82-83	-0.44140251959877475
2214	84-85	-0.45264640414866264
2214	86-87	-0.4158324545627998
2214	88-89	-0.3919654215066579
2214	90-91	-0.37725162723813455
2214	92-93	-0.3452806919255842
2214	94-95	-0.30601698360155893
2214	96-97	-0.2948742892607292
2214	98-99	-0.298957837022904
2214	100-101	-0.14535746909218972
2213	1	-3.314972808116682
2213	2	-1.5988675983901928
2213	3	-2.8463667463987363
2213	4	-1.12879602382802
2213	5	0.362675119598066
2213	6	-0.13987586822483422
2213	7	-0.20606086191387618
2213	8	-0.39933727485001214
2213	9	-0.5198494871952022
2213	10-11	-0.4134782130606993
2213	12-13	-0.5996324657608909
2213	14-15	-0.6582165771529347
2213	16-17	-0.5515301869347553
2213	18-19	-0.5592582397626913
2213	20-21	-0.6927746021328502
2213	22-23	-0.6147498144091372
2213	24-25	-0.6197185305600144
2213	26-27	-0.5050652179063491
2213	28-29	-0.6306437008671892
2213	30-31	-0.5951977198819733
2213	32-33	-0.5998117616616483
2213	34-35	-0.9062725678741685
2213	36-37	-0.8634413552048272
2213	38-39	-0.9425621899564263
2213	40-41	-0.7430086186368783
2213	42-43	-0.6387639308054602
2213	44-45	-0.6733113690788315
2213	46-47	-0.6290056850738708
2213	48-49	-0.6257234472201461
2213	50-51	-0.6096664465394568
2213	52-53	-0.5571326371418479
2213	54-55	-0.4361535179288296
2213	56-57	-0.47234660676472373
2213	58-59	-0.489068057924392
2213	60-61	-0.38012135699802485
2213	62-63	-0.43679543747402505
2213	64-65	-0.2094081095448317
2213	66-67	-0.2907159395564136
2213	68-69	-0.03690226074613889
2213	70-71	1.060184247196343
2213	72-73	-0.2521273631922014
2213	74-75	-0.506132196791576
2213	76-77	-0.19295795652459447
2213	78-79	-0.318963392481983
2213	80-81	-0.35883312685142954
2213	82-83	-0.3456204530282463
2213	84-85	-0.3164823602309532
2213	86-87	-0.32850482230301026
2213	88-89	-0.35310054590032536
2213	90-91	-0.3899352281287918
2213	92-93	-0.3507904795165935
2213	94-95	-0.357354190800212
2213	96-97	-0.3857739737682664
2213	98-99	-0.40719966765805893
2213	100-101	-0.10523470021665915
2212	1	-4.060389369828158
2212	2	-5.814723338117233
2212	3	-0.34690533001253243
2212	4	2.0633493475010845
2212	5	-4.175240363183502
2212	6	-1.9053380283476997
2212	7	-1.3086227636811216
2212	8	-0.9905392814227127
2212	9	-1.2666190942941356
2212	10-11	-1.1211498406252787
2212	12-13	-0.9743962668238453
2212	14-15	-0.9773961980860477
2212	16-17	-1.1389008409131662
2212	18-19	-0.963305989589692
2212	20-21	-1.4535786707362988
2212	22-23	-1.1514573458421538
2212	24-25	-0.9960679795144571
2212	26-27	-1.0289720823266038
2212	28-29	-1.3736610295144516
2212	30-31	-1.420447608008736
2212	32-33	-1.2042467321822699
2212	34-35	-1.450629646004998
2212	36-37	-1.7090597104512852
2212	38-39	-1.663428850709586
2212	40-41	-1.4744337007198922
2212	42-43	-1.3174176271899327
2212	44-45	-1.3764199144500964
2212	46-47	-1.3056467246676107
2212	48-49	-1.30725968230157
2212	50-51	-1.2413912361125838
2212	52-53	-1.4568297833651371
2212	54-55	-1.2459742298197654
2212	56-57	-1.2865009872770088
2212	58-59	-1.2144946640610499
2212	60-61	-0.9596780565888778
2212	62-63	-1.330497405665831
2212	64-65	-0.8143863404636917
2212	66-67	-0.8462276176814356
2212	68-69	-0.2840274787828534
2212	70-71	1.352906995284357
2212	72-73	0.24068762226759688
2212	74-75	-0.018928480799665692
2212	76-77	0.24656079145803744
2212	78-79	-0.008340856085986559
2212	80-81	-0.08443254183166005
2212	82-83	-0.04188634153863191
2212	84-85	-0.06904203021960598
2212	86-87	-0.14511912509443192
2212	88-89	-0.16334061893966378
2212	90-91	-0.2513059078840101
2212	92-93	-0.1743439031786771
2212	94-95	-0.30842972802391344
2212	96-97	-0.17418359886547208
2212	98-99	-0.17305067374005745
2212	100-101	-0.027902254666841486
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4105.0
3	224.0
4	270.0
5	508.0
6	844.0
7	1843.0
8	3551.0
9	5818.0
10	8167.0
11	10598.0
12	13439.0
13	16859.0
14	19234.0
15	20150.0
16	22588.0
17	25643.0
18	31326.0
19	39647.0
20	51766.0
21	66877.0
22	86609.0
23	113880.0
24	158340.0
25	244809.0
26	392862.0
27	575849.0
28	248088.0
29	187671.0
30	231973.0
31	289224.0
32	360789.0
33	461005.0
34	612338.0
35	859103.0
36	1308590.0
37	1885059.0
38	1273820.0
39	119122.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.50188521298021	22.644765500135914	11.346540710315809	40.50680857656807
2	14.84860273524248	37.25433358277226	20.465962044403955	27.431101637581296
3	10.608205273035496	15.469343516833018	39.459676862779844	34.46277434735164
4	13.624933663881617	16.99774649476105	12.45936640354408	56.91795343781325
5	31.653341656594126	20.4379083787811	15.3785641308748	32.530185833749975
6	30.076686004704168	26.1555476283467	17.68025169013436	26.08751467681477
7	7.662471146226903	48.02279878728022	22.60674665607943	21.70798341041344
8	7.871576266523634	49.29159043502254	19.722585343980864	23.114247954472958
9	22.36346044842049	37.85196652554921	20.676295383646178	19.108277642384127
10-11	14.873551083737993	43.24533249171454	22.53455322941474	19.34656319513272
12-13	6.845314814438848	45.73459846847528	20.22104489925491	27.199041817830956
14-15	7.65392041192583	42.486587847923474	20.323334337677853	29.53615740247284
16-17	15.047932701654283	36.25014765244911	20.444645096831294	28.25727454906532
18-19	10.26490923527146	31.448819646075055	28.346186908327926	29.94008421032556
20-21	19.711089199071903	31.55758147072028	28.324299615811306	20.40702971439651
22-23	6.701234046859758	49.027259238022644	20.984600228022483	23.286906487095116
24-25	6.802638222230972	33.82692849558088	28.29343024259717	31.077003039590974
26-27	7.389866072994083	33.52207907900908	23.396216567722377	35.69183828027446
28-29	14.619432777439064	41.27696441985469	20.235329667839487	23.868273134866755
30-31	6.744358535801455	30.806804399283124	31.285664093679767	31.16317297123566
32-33	7.001517341144186	41.55829028321309	21.922734134310634	29.517458241332093
34-35	8.153193538402743	33.28995920114282	36.08755483866899	22.469292421785443
36-37	14.655277337409931	41.09238182023442	21.325097699798174	22.927243142557472
38-39	14.503463900119748	33.232077437228135	22.10478759922373	30.159671063428384
40-41	7.054612271432043	40.554300046039145	29.512677171248193	22.878410511280617
42-43	7.460598429505293	32.742385401743384	29.73952207961498	30.05749408913635
44-45	14.818366801680336	33.19831966353894	22.15174248540719	29.831571049373533
46-47	7.688242345384033	40.08785314491759	29.108127728554006	23.11577678114437
48-49	15.226670787928859	32.68921177578884	29.05843653068211	23.025680905600193
50-51	7.200690872149165	32.72624242371406	22.108195952921	37.96487075121577
52-53	14.567710616655106	32.64201901480519	30.268949424912446	22.521320943627256
54-55	6.965302536307397	32.70796613717615	30.068778445445222	30.257952881071233
56-57	7.457436660050108	32.601036231177815	29.486416044782526	30.45511106398955
58-59	14.755096839658721	32.696292355062226	30.229611500636477	22.31899930464258
60-61	7.474652705146978	33.48916997863116	29.468373302490026	29.567804013731834
62-63	14.656862657136434	34.24779716118143	29.096995659305886	21.998344522376247
64-65	7.035233073863072	49.363114488046314	21.39931628802609	22.202336150064518
66-67	7.012649024098098	49.42747194396427	21.567124667578536	21.992754364359097
68-69	7.055763218840908	49.401770292978775	21.632915807341586	21.90955068083873
70-71	7.256403562505549	48.33874769263392	21.893502379194093	22.511346365666434
72-73	7.6168280406779605	46.46888117972197	22.562166223725093	23.35212455587498
74-75	8.026647623568019	44.85406964460658	22.913019888154096	24.206262843671304
76-77	8.385719556295031	43.29183759310419	23.266727480008196	25.05571537059258
78-79	8.721523591241352	42.28590776334715	23.557741654889135	25.434826990522364
80-81	9.198671466841963	41.40090697207159	23.68803556768502	25.712385993401426
82-83	9.485589713537461	40.707801852600966	23.516968161657303	26.289640272204267
84-85	9.933179883686426	39.66210760984607	23.75793147305714	26.646781033410367
86-87	10.316252787518602	39.41335079461423	23.677065216951064	26.593331200916104
88-89	10.683080607595887	38.97381098708773	23.389019864516232	26.954088540800146
90-91	11.088142134245931	38.61824933262166	23.35429696089521	26.939311572237205
92-93	11.461302612101433	38.36408783884417	23.201160197974392	26.97344935108001
94-95	11.851632879029097	38.18661074725108	22.841808786380213	27.11994758733961
96-97	12.211053305494353	37.72952867273946	22.806374254839458	27.253043766926726
98-99	12.56327924692941	37.43686943129554	22.62350930853859	27.376342013236467
100-101	13.144725073961217	36.967878491609014	22.220674483232767	27.666721951197005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.0
4	3.0
5	8.0
6	16.0
7	16.0
8	30.0
9	52.5
10	83.0
11	148.5
12	270.0
13	481.0
14	845.5
15	1458.0
16	2346.0
17	3554.5
18	5345.5
19	8157.5
20	12356.0
21	18953.5
22	29518.0
23	46119.5
24	71082.5
25	105363.0
26	149804.0
27	203530.0
28	263367.0
29	322007.0
30	373793.0
31	417122.5
32	448452.0
33	467444.5
34	473085.5
35	467921.0
36	457730.0
37	445995.0
38	435784.0
39	428935.0
40	425249.0
41	420501.0
42	413177.0
43	400577.5
44	379924.0
45	350910.0
46	314342.5
47	273871.5
48	230953.5
49	189970.0
50	156554.0
51	126429.5
52	95026.5
53	69015.0
54	51332.0
55	38636.0
56	29408.5
57	22458.0
58	17460.0
59	13870.0
60	11356.5
61	9509.0
62	7920.5
63	6711.0
64	5683.0
65	4789.5
66	4107.0
67	3539.5
68	3035.0
69	2555.5
70	2141.0
71	1819.5
72	1533.5
73	1262.5
74	1033.5
75	822.0
76	639.5
77	510.5
78	434.5
79	354.5
80	291.5
81	248.5
82	204.0
83	166.5
84	156.5
85	139.5
86	129.5
87	123.0
88	98.0
89	86.0
90	80.0
91	73.5
92	64.0
93	50.5
94	38.0
95	27.5
96	21.5
97	13.5
98	8.5
99	6.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015893142303915026
2	8.202912156859367E-4
3	0.012027519949995048
4	0.01188396898725001
5	4.716674490194136E-4
6	7.177548137251946E-5
7	1.0253640196074208E-4
8	1.3329732254896472E-4
9	2.050728039214842E-5
10-11	0.0
12-13	0.0
14-15	4.6141380882333944E-5
16-17	2.050728039214842E-5
18-19	3.024823857841892E-4
20-21	4.6141380882333944E-5
22-23	8.920666970584563E-4
24-25	6.152184117644525E-5
26-27	8.202912156859368E-5
28-29	6.152184117644526E-4
30-31	9.27954437744716E-4
32-33	4.92174729411562E-4
34-35	0.002845385154410593
36-37	5.639502107840815E-4
38-39	0.001184295442646571
40-41	6.562329725487494E-4
42-43	0.006921207132350091
44-45	0.0067520220691148665
46-47	0.013955204306856999
48-49	0.016159736949012956
50-51	0.027059356477439837
52-53	7.741498348036028E-4
54-55	0.005367780642644849
56-57	8.254180357839737E-4
58-59	9.79222638725087E-4
60-61	0.0011689149823524599
62-63	0.0016559628916659847
64-65	0.0013893682465680555
66-67	0.0014611437279405748
68-69	6.921207132350091E-4
70-71	0.001763626113724764
72-73	3.178628460783005E-4
74-75	1.999459838234471E-4
76-77	0.0039784123960767935
78-79	0.003147867540194782
80-81	7.536425544114544E-4
82-83	0.0040912024382336095
84-85	3.281164862743747E-4
86-87	8.715594166663078E-5
88-89	0.00405531469754735
90-91	0.0011022663210779775
92-93	0.0016457092514699106
94-95	2.9222874558811496E-4
96-97	0.0012458172838230165
98-99	2.9735556568615207E-4
100-101	5.793306710781929E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9752634.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.28844701414856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.93128940894572	63.004878281399435
2	6.021131085197473	8.34389644323896
3	1.379559329168353	2.8676257044586726
4	0.563595507843649	1.5620262993054728
5	0.3252807122582291	1.1269097698014405
6	0.16374325229443748	0.6807309396316493
7	0.13164370648712675	0.6384971587175585
8	0.1075615617035897	0.5962218855086597
9	0.06661826075989825	0.41542882477532483
>10	0.288148629585429	3.3418501253308546
>50	0.011534681125900914	0.5487971492751113
>100	0.008203143097500738	1.147578055597421
>500	8.350856748052868E-4	0.3967093252319841
>1k	6.307373423132389E-4	0.837367796311643
>5k	1.350493056021335E-4	0.635006896945455
>10k+	8.9849210030101E-5	13.85647534447032
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	1099779	11.276738161198297	TruSeq Adapter, Index 3 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	71988	0.7381390504349902	TruSeq Adapter, Index 3 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT	64352	0.6598422538977675	TruSeq Adapter, Index 3 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	46999	0.48191083557529174	TruSeq Adapter, Index 3 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	30757	0.31537121151065445	TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	21579	0.22126330179108536	TruSeq Adapter, Index 3 (98% over 50bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.998919676468942E-4	0.0	0.0
2	5.536965705880073E-4	0.0	1.025364019607421E-5
3	6.049647715683784E-4	0.0	1.025364019607421E-5
4	6.664866127448236E-4	0.0	1.025364019607421E-5
5	8.715594166663078E-4	0.0	1.025364019607421E-5
6	0.0011381540617642373	0.0	1.025364019607421E-5
7	0.0014560169078425378	0.0	1.025364019607421E-5
8	0.0020302207588226936	0.0	1.025364019607421E-5
9	0.0021840253617638064	0.0	1.025364019607421E-5
10-11	0.0039117637348023105	0.0	1.025364019607421E-5
12-13	0.007572313284800803	0.0	1.025364019607421E-5
14-15	0.013965457947053072	5.126820098037105E-6	1.025364019607421E-5
16-17	0.017508090634796712	1.025364019607421E-5	1.025364019607421E-5
18-19	0.018810302939698136	1.025364019607421E-5	1.025364019607421E-5
20-21	0.02031246122842301	1.025364019607421E-5	1.025364019607421E-5
22-23	0.02663383040930276	1.025364019607421E-5	1.025364019607421E-5
24-25	0.03885616952302322	2.050728039214842E-5	1.025364019607421E-5
26-27	0.042701284596551044	3.076092058822263E-5	1.025364019607421E-5
28-29	0.04441364250929544	4.6141380882333944E-5	1.025364019607421E-5
30-31	0.045859405776941906	5.126820098037105E-5	1.025364019607421E-5
32-33	0.04794089473674497	5.126820098037105E-5	1.025364019607421E-5
34-35	0.04999162277595981	5.126820098037105E-5	1.025364019607421E-5
36-37	0.05257041328527247	5.126820098037105E-5	1.025364019607421E-5
38-39	0.05567726626468296	5.126820098037105E-5	1.025364019607421E-5
40-41	0.05990689284556357	5.126820098037105E-5	1.025364019607421E-5
42-43	0.06491579608134582	5.126820098037105E-5	1.025364019607421E-5
44-45	0.0716831986107548	5.126820098037105E-5	1.025364019607421E-5
46-47	0.07999377398967294	5.126820098037105E-5	1.025364019607421E-5
48-49	0.09037045786810005	5.126820098037105E-5	1.025364019607421E-5
50-51	0.10441794493672171	7.177548137251946E-5	1.025364019607421E-5
52-53	0.12319748695583163	7.177548137251946E-5	1.5380460294111313E-5
54-55	0.14938528401660517	7.177548137251946E-5	2.050728039214842E-5
56-57	0.1854422097661001	7.177548137251946E-5	2.050728039214842E-5
58-59	0.23293194433421782	7.177548137251946E-5	2.050728039214842E-5
60-61	0.2961507629631134	7.177548137251946E-5	4.101456078429684E-5
62-63	0.3839578107821948	7.690230147055657E-5	4.101456078429684E-5
64-65	0.5018080243757738	8.715594166663078E-5	4.101456078429684E-5
66-67	0.6531978950507115	9.228276176466789E-5	4.101456078429684E-5
68-69	0.8500216454344539	1.025364019607421E-4	4.101456078429684E-5
70-71	1.1002924953402333	1.025364019607421E-4	4.101456078429684E-5
72-73	1.4170223141768674	1.025364019607421E-4	4.101456078429684E-5
74-75	1.8133152541149395	1.025364019607421E-4	4.101456078429684E-5
76-77	2.2964872874343483	1.025364019607421E-4	4.101456078429684E-5
78-79	2.8753104033228354	1.025364019607421E-4	4.101456078429684E-5
80-81	3.5645395900225516	1.0766322205877919E-4	4.101456078429684E-5
82-83	4.3722085746271215	1.127900421568163E-4	4.101456078429684E-5
84-85	5.298322483956642	1.127900421568163E-4	4.101456078429684E-5
86-87	6.35978957069444	1.127900421568163E-4	4.101456078429684E-5
88-89	7.545751229872874	1.230436823528905E-4	4.101456078429684E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGA	8890	0.0	86.83572	1
CCGGAAG	9465	0.0	86.37656	3
ACCGGAA	10020	0.0	81.30861	2
CATCGGA	3655	0.0	70.70634	1
ACGGAAG	4935	0.0	70.462746	3
GATTGGA	7795	0.0	69.35423	1
ATTGGAA	7895	0.0	68.77557	2
TTGGAAG	7970	0.0	68.4257	3
TGGAAGA	8760	0.0	66.91275	4
GAGCACA	226195	0.0	63.276356	9
GAACGGA	5355	0.0	62.276535	1
AGAGCAC	230820	0.0	62.028732	8
AAGAGCA	236315	0.0	60.65472	7
GCGGAAG	2735	0.0	60.444607	3
GAAGAGC	240065	0.0	59.74286	6
AGCGGAA	2730	0.0	59.685883	2
GAGCGGA	2550	0.0	59.615147	1
AATCATA	84085	0.0	57.8688	5
GGAAGAG	245220	0.0	57.444923	5
CGGAAGA	242980	0.0	55.537106	4
>>END_MODULE