##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2212_lane2_CTTGTA_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.9359574375 31.0 28.0 33.0 16.0 34.0 2 29.7545958125 31.0 30.0 34.0 25.0 34.0 3 30.5307106875 31.0 30.0 34.0 26.0 34.0 4 33.452253875 35.0 33.0 37.0 28.0 37.0 5 34.1154691875 35.0 35.0 37.0 28.0 37.0 6 34.8610655625 35.0 35.0 37.0 32.0 37.0 7 34.9749043125 35.0 35.0 37.0 32.0 37.0 8 34.990649375 36.0 35.0 37.0 32.0 37.0 9 36.62342375 39.0 35.0 39.0 32.0 39.0 10-11 36.647454374999995 39.0 35.0 39.0 32.0 39.0 12-13 36.721677906249994 39.0 35.0 39.0 32.0 39.0 14-15 37.75430003125 39.5 37.0 41.0 32.0 41.0 16-17 37.76582728125 39.0 37.0 41.0 32.0 41.0 18-19 37.87604925 39.5 37.0 41.0 32.5 41.0 20-21 38.08987346875 40.0 37.0 41.0 33.0 41.0 22-23 38.12607321875 40.0 37.0 41.0 33.0 41.0 24-25 38.02313215625 40.0 37.0 41.0 33.0 41.0 26-27 37.8759653125 40.0 37.0 41.0 33.0 41.0 28-29 37.708435375 40.0 37.0 41.0 32.0 41.0 30-31 37.6465043125 40.0 37.0 41.0 32.0 41.0 32-33 37.5223689375 40.0 37.0 41.0 32.0 41.0 34-35 37.42483903125 40.0 37.0 41.0 31.0 41.0 36-37 37.30641390625 40.0 36.0 41.0 31.0 41.0 38-39 37.206926531250005 39.5 36.0 41.0 31.0 41.0 40-41 37.0838835 39.0 36.0 41.0 31.0 41.0 42-43 36.9601715625 39.0 35.5 41.0 30.5 41.0 44-45 36.81778340625 39.0 35.0 41.0 30.0 41.0 46-47 36.64661715625 39.0 35.0 41.0 30.0 41.0 48-49 36.498950468749996 39.0 35.0 40.5 30.0 41.0 50-51 36.34639184375 39.0 35.0 40.0 30.0 41.0 52-53 36.147633562500005 38.5 35.0 40.0 29.5 41.0 54-55 35.95650725 38.0 34.0 40.0 29.0 41.0 56-57 35.768620312500005 38.0 34.0 40.0 28.0 41.0 58-59 35.55272025 38.0 34.0 40.0 28.0 41.0 60-61 35.307693281249996 37.0 34.0 40.0 28.0 41.0 62-63 35.001910875 37.0 34.0 40.0 28.0 41.0 64-65 34.65025878125 36.0 33.0 39.0 28.0 41.0 66-67 34.2266261875 36.0 33.0 39.0 27.5 40.0 68-69 33.8203663125 35.0 33.0 38.5 26.5 40.0 70-71 33.4188999375 35.0 33.0 37.0 26.0 39.0 72-73 33.0203338125 35.0 33.0 37.0 26.0 39.0 74-75 32.5932718125 35.0 32.0 36.0 26.0 38.0 76-77 29.79608075 32.0 28.0 34.5 21.5 35.5 78-79 31.773068281249998 34.0 31.5 35.0 25.5 37.0 80-81 31.922862000000002 34.5 32.0 35.0 26.0 36.0 82-83 31.77598453125 34.0 32.0 35.0 25.5 36.0 84-85 31.597105125 34.0 32.0 35.0 25.0 36.0 86-87 31.4480249375 34.0 32.0 35.0 25.0 35.0 88-89 31.2587945 34.0 32.0 35.0 24.5 35.0 90-91 31.0976895 34.0 32.0 35.0 24.0 35.0 92-93 30.8724334375 34.0 31.5 35.0 23.5 35.0 94-95 30.63991653125 34.0 31.0 35.0 23.0 35.0 96-97 30.39983759375 34.0 31.0 35.0 20.0 35.0 98-99 30.0967596875 34.0 31.0 35.0 16.5 35.0 100-101 28.73589446875 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 2.3957117618181343 1101 2 1.9375053038197585 1101 3 1.4297456093439322 1101 4 1.5325540119016026 1101 5 1.542673521630931 1101 6 0.7713593878767355 1101 7 0.37080116957780973 1101 8 0.1835714115358016 1101 9 0.12127143637986393 1101 10-11 0.07231143762724201 1101 12-13 0.11171888165107902 1101 14-15 0.08197209746041523 1101 16-17 0.045557414564584064 1101 18-19 0.21490677439823713 1101 20-21 0.4087367303928957 1101 22-23 0.42264585510667274 1101 24-25 0.41856018993595256 1101 26-27 0.42384004928712926 1101 28-29 0.35721697888905624 1101 30-31 0.37587937810506133 1101 32-33 0.449098479039705 1101 34-35 0.4887260809647316 1101 36-37 0.4069216397575417 1101 38-39 0.4685773856446005 1101 40-41 0.5136301136791843 1101 42-43 0.5383470633120737 1101 44-45 0.4154674948766939 1101 46-47 0.4378708669990843 1101 48-49 0.42073866779975333 1101 50-51 0.47686158846345705 1101 52-53 0.46664516250375243 1101 54-55 0.444626272774336 1101 56-57 0.44940140513837434 1101 58-59 0.3938223838728021 1101 60-61 0.40756808328924166 1101 62-63 0.4534308765584356 1101 64-65 0.39142384733629143 1101 66-67 0.31581740472578446 1101 68-69 0.33705925404489534 1101 70-71 0.3057483595416812 1101 72-73 0.25915136310815257 1101 74-75 0.24560885730137727 1101 76-77 0.13448477488532973 1101 78-79 0.1479879241430062 1101 80-81 0.1466020154120038 1101 82-83 0.16755574403441287 1101 84-85 0.06923006790577446 1101 86-87 0.1543959167132627 1101 88-89 0.126548732391619 1101 90-91 0.03655851720308334 1101 92-93 0.07088245626987444 1101 94-95 0.03686261137322688 1101 96-97 -0.03436102009451858 1101 98-99 -0.024035533609701787 1101 100-101 -0.1477191773375992 1102 1 2.2002038035826743 1102 2 1.8775394673595933 1102 3 1.440903555650067 1102 4 0.5591512312114091 1102 5 1.2764552995966056 1102 6 0.6236374566557572 1102 7 0.3657466055449916 1102 8 0.1968932825165126 1102 9 0.18667993693965457 1102 10-11 0.11024443743575318 1102 12-13 0.15202034528689978 1102 14-15 0.16068435544885062 1102 16-17 0.18827963771666134 1102 18-19 0.22929483028470798 1102 20-21 0.37222212698258517 1102 22-23 0.3501853234874446 1102 24-25 0.37524452622991333 1102 26-27 0.26071757622619174 1102 28-29 0.24630706494364318 1102 30-31 0.18180014540308775 1102 32-33 0.25889325720590506 1102 34-35 0.2838047997237325 1102 36-37 0.3494687503902725 1102 38-39 0.39179628439197245 1102 40-41 0.39623148220069027 1102 42-43 0.1516719053996951 1102 44-45 0.3567362481793097 1102 46-47 0.43238962730033137 1102 48-49 0.38432086887564765 1102 50-51 0.3043497590667883 1102 52-53 0.30206183805351117 1102 54-55 0.34794173392820227 1102 56-57 0.30697777584432373 1102 58-59 0.2144287482026641 1102 60-61 0.26672947774817857 1102 62-63 0.206284895481609 1102 64-65 0.2518420339839196 1102 66-67 0.2462317756921948 1102 68-69 0.22935285343808687 1102 70-71 0.20407353842664833 1102 72-73 0.17226916392801428 1102 74-75 0.13322969854986155 1102 76-77 -0.08886221556951313 1102 78-79 0.10937517424731524 1102 80-81 0.08736229435826814 1102 82-83 0.10678169207101718 1102 84-85 0.07162936180657908 1102 86-87 0.08979986372069604 1102 88-89 0.058174631554070544 1102 90-91 0.03511650945875289 1102 92-93 0.05272394957058779 1102 94-95 0.02051865690986787 1102 96-97 -0.0554414116537032 1102 98-99 -0.12484328895994778 1102 100-101 -0.1661649207958753 1103 1 2.217118499300735 1103 2 1.8261217469917526 1103 3 1.3045145412772676 1103 4 0.8641899716551436 1103 5 1.2894810713635252 1103 6 0.600176149966785 1103 7 0.3433684638845591 1103 8 0.22634765778720123 1103 9 0.1910840094587769 1103 10-11 0.11203230788807161 1103 12-13 0.14870926335227352 1103 14-15 0.20233889152354578 1103 16-17 0.20138263167849146 1103 18-19 0.1981748128375429 1103 20-21 0.3152958882410246 1103 22-23 0.14491597960327596 1103 24-25 0.33259423623858453 1103 26-27 0.3233360749967673 1103 28-29 0.2935003552452997 1103 30-31 0.3636417473626352 1103 32-33 0.37237291572338904 1103 34-35 0.26024531464670275 1103 36-37 0.33727731879424994 1103 38-39 0.3615441100859158 1103 40-41 0.38721570793582316 1103 42-43 0.4060175328898694 1103 44-45 0.4499066753212233 1103 46-47 0.4232831634159666 1103 48-49 0.26361880850383557 1103 50-51 0.4448865887161091 1103 52-53 0.31905173954051236 1103 54-55 0.3470584257310847 1103 56-57 0.37126177355421675 1103 58-59 0.401625363588181 1103 60-61 0.38295584844936315 1103 62-63 0.3997155342366412 1103 64-65 0.45476358285754515 1103 66-67 0.3787928489747827 1103 68-69 0.30152738966828707 1103 70-71 0.3195300960118601 1103 72-73 0.27819760789407155 1103 74-75 0.22699981892005638 1103 76-77 0.10949985388707617 1103 78-79 0.2798827994989601 1103 80-81 0.25432381455716424 1103 82-83 0.2231331164934005 1103 84-85 0.2400966598464329 1103 86-87 0.24793531601251217 1103 88-89 0.15186671957092912 1103 90-91 0.1705434631086078 1103 92-93 0.19767630672436454 1103 94-95 0.26831783830846945 1103 96-97 0.09302550506208362 1103 98-99 0.2498564604857556 1103 100-101 0.13026780814285743 1116 1 1.8384761172240367 1116 2 1.5996328606499262 1116 3 0.9564445771114798 1116 4 0.11374791342150559 1116 5 1.0232026654427955 1116 6 0.48215322474425193 1116 7 0.1937482886965185 1116 8 0.0620425337389392 1116 9 0.10086505545921653 1116 10-11 0.11972294790572136 1116 12-13 0.09515315585905171 1116 14-15 0.07884107373227067 1116 16-17 0.09848994458911875 1116 18-19 0.18894412719095754 1116 20-21 0.30547393297359093 1116 22-23 0.1577672429904311 1116 24-25 0.2454036337445089 1116 26-27 0.23538100652054794 1116 28-29 0.22641274933777567 1116 30-31 0.22270623909800236 1116 32-33 0.14093176752978565 1116 34-35 0.19076593116361806 1116 36-37 0.18321923736362322 1116 38-39 0.17593553581796328 1116 40-41 0.0433297379858999 1116 42-43 0.07898358720126453 1116 44-45 0.1821939750238002 1116 46-47 0.21540040462105736 1116 48-49 0.2574035964296968 1116 50-51 0.2960786895059755 1116 52-53 0.29680803152002966 1116 54-55 0.34529138532301573 1116 56-57 0.3031311854293435 1116 58-59 0.2937990032344828 1116 60-61 0.10563133135405423 1116 62-63 0.22253720879556482 1116 64-65 0.3015534374977804 1116 66-67 0.2038274830186424 1116 68-69 0.18565645485201543 1116 70-71 0.2100396651619434 1116 72-73 0.26641706233647255 1116 74-75 0.26125205912332206 1116 76-77 0.059093072818850345 1116 78-79 0.15615480309990204 1116 80-81 0.23516748176093571 1116 82-83 0.13282211218982098 1116 84-85 0.19950862809139736 1116 86-87 0.19556835231701086 1116 88-89 0.23738102852473375 1116 90-91 0.22361568882151772 1116 92-93 0.17147393886217444 1116 94-95 0.0606202744217903 1116 96-97 0.13454808452718936 1116 98-99 0.12626548935280013 1116 100-101 0.08248030719024158 1113 1 0.9234815906051068 1113 2 1.0647582865111573 1113 3 0.6304543814119796 1113 4 1.3174394238860785 1113 5 1.2891482027251584 1113 6 0.5013018924512593 1113 7 0.34068754486794006 1113 8 0.15271864131000257 1113 9 0.1434613865844412 1113 10-11 0.10359916894999088 1113 12-13 0.09880205500456896 1113 14-15 0.14697389536934935 1113 16-17 0.08055407602920894 1113 18-19 0.17172713404522 1113 20-21 0.2257404441099311 1113 22-23 0.1576964116256292 1113 24-25 0.17024756600611823 1113 26-27 0.07378685908560811 1113 28-29 0.11255333913077692 1113 30-31 0.20514816730878493 1113 32-33 0.18577420431271463 1113 34-35 0.22643741164244346 1113 36-37 0.24937929529078673 1113 38-39 0.2044624112296276 1113 40-41 0.19585317763153398 1113 42-43 0.20686393401038572 1113 44-45 0.23768182257838077 1113 46-47 0.3027335959397277 1113 48-49 0.28520854733204715 1113 50-51 0.1584727578580427 1113 52-53 0.15008610084585428 1113 54-55 0.3035076052076917 1113 56-57 0.19059661219363733 1113 58-59 0.18286541354524388 1113 60-61 0.21058199168480485 1113 62-63 0.14784086659364704 1113 64-65 0.2066697177502661 1113 66-67 0.28493926317524654 1113 68-69 0.15768949553953604 1113 70-71 0.14407332401190587 1113 72-73 0.17352620666770946 1113 74-75 0.13077570635639546 1113 76-77 -0.030105915333024313 1113 78-79 0.09824985384161877 1113 80-81 0.20658216603899504 1113 82-83 0.18542171355362314 1113 84-85 0.17510726378143815 1113 86-87 0.15900289998716488 1113 88-89 0.12342233613976816 1113 90-91 0.0756840971334043 1113 92-93 0.07192285875543547 1113 94-95 0.07764484247470449 1113 96-97 0.04550428767994319 1113 98-99 0.12685576980901558 1113 100-101 0.16122452623027428 1112 1 1.9559585021660517 1112 2 1.732096365366484 1112 3 1.3019471288537758 1112 4 1.6764639372888297 1112 5 1.4625407604539404 1112 6 0.6112812258369473 1112 7 0.28592790454014505 1112 8 0.12226629474461959 1112 9 0.1166731020328271 1112 10-11 0.043684893059996455 1112 12-13 -0.0011449912672674145 1112 14-15 -0.05804076457857121 1112 16-17 0.010922294427800239 1112 18-19 -0.0037161442646365117 1112 20-21 0.03044324080277505 1112 22-23 0.1883833115230047 1112 24-25 0.008163225949665787 1112 26-27 0.1053286439418244 1112 28-29 0.12471062380834042 1112 30-31 0.043547395138894274 1112 32-33 0.011879436817785916 1112 34-35 0.1385131986608812 1112 36-37 0.06589538093713543 1112 38-39 -0.04933159853315772 1112 40-41 0.046971613099699994 1112 42-43 0.1260280376808396 1112 44-45 0.1498582896632783 1112 46-47 -0.00291071240517482 1112 48-49 -0.054250331306221256 1112 50-51 0.011790305376337074 1112 52-53 0.05725136730151803 1112 54-55 0.16000723320215826 1112 56-57 0.17751974654853342 1112 58-59 0.1357905293272239 1112 60-61 0.17043520756588038 1112 62-63 0.14380743987522493 1112 64-65 0.14494651360201516 1112 66-67 0.11224064275359069 1112 68-69 0.143194905989958 1112 70-71 0.05824714897414651 1112 72-73 0.18846500664123056 1112 74-75 0.21690927002430982 1112 76-77 0.21989338802488234 1112 78-79 0.24486088857650756 1112 80-81 0.27228563370221437 1112 82-83 0.1866488697806119 1112 84-85 0.1817394192994506 1112 86-87 0.18036941328104206 1112 88-89 0.20204841417255537 1112 90-91 0.18000436937015252 1112 92-93 0.1120764076498304 1112 94-95 0.18601657059691235 1112 96-97 0.20601958126407638 1112 98-99 0.16851606115924866 1112 100-101 0.22553398947237824 1115 1 1.0917284847701758 1115 2 1.3706639321543221 1115 3 1.141833013182783 1115 4 1.4663392590255597 1115 5 1.4076042952663386 1115 6 0.6118746522234275 1115 7 0.2156352099996255 1115 8 0.0884281709104826 1115 9 0.1046434633359965 1115 10-11 0.06071558905691177 1115 12-13 0.08479836202664615 1115 14-15 0.03935597072484853 1115 16-17 0.10113512973679661 1115 18-19 0.07530464501908085 1115 20-21 0.12261811557369384 1115 22-23 0.1223025921515628 1115 24-25 0.09566908292227083 1115 26-27 0.12666379195920996 1115 28-29 0.1680059683562618 1115 30-31 0.1448126669475016 1115 32-33 0.19626690397384294 1115 34-35 0.20491238628095942 1115 36-37 0.1500813818528428 1115 38-39 0.20891808986228 1115 40-41 0.23952400924731876 1115 42-43 0.14834809620373335 1115 44-45 0.191210912929769 1115 46-47 0.23275909491470514 1115 48-49 0.06809484345346561 1115 50-51 0.16255389224626526 1115 52-53 0.17924806704530738 1115 54-55 0.14632689646744979 1115 56-57 0.18003590950660708 1115 58-59 0.2261348269165353 1115 60-61 0.24940519121918925 1115 62-63 0.2935080649057866 1115 64-65 0.28107347489746104 1115 66-67 0.27988452088364113 1115 68-69 0.33115067493380224 1115 70-71 0.35534196611348534 1115 72-73 0.35293733155454277 1115 74-75 0.27905938531346663 1115 76-77 0.4046499305355411 1115 78-79 0.29815696706576134 1115 80-81 0.29996465153289975 1115 82-83 0.3614657616824246 1115 84-85 0.31926873622597896 1115 86-87 0.3551100728102199 1115 88-89 0.30576082056694176 1115 90-91 0.2734681199480633 1115 92-93 0.3232177940947025 1115 94-95 0.3888873804217994 1115 96-97 0.32496730483304503 1115 98-99 0.2953656198294752 1115 100-101 0.27043296132761085 1114 1 0.020614812209682754 1114 2 0.6019625125247998 1114 3 0.39042922510850886 1114 4 0.2956578944708923 1114 5 0.9077827231551012 1114 6 0.36122528145771327 1114 7 0.14041337890612482 1114 8 0.08293238940672865 1114 9 0.15048377615507746 1114 10-11 -0.0010863372846188213 1114 12-13 -0.04427117707228945 1114 14-15 -0.058719889426285476 1114 16-17 -0.02459743017890048 1114 18-19 -0.029290273932268462 1114 20-21 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-0.2336779046319748 1209 22-23 -0.26506011672141483 1209 24-25 -0.25756997428175765 1209 26-27 -0.22809004231344687 1209 28-29 -0.30983525971866754 1209 30-31 -0.33478451829215317 1209 32-33 -0.2750423887370843 1209 34-35 -0.2520543402388782 1209 36-37 -0.2589945756458292 1209 38-39 -0.1973354829616909 1209 40-41 -0.25687832148358325 1209 42-43 -0.22222287489475434 1209 44-45 -0.2730511995578482 1209 46-47 -0.2580391660333916 1209 48-49 -0.23591183007187766 1209 50-51 -0.2301009543091297 1209 52-53 -0.23801410928926714 1209 54-55 -0.24068016582317142 1209 56-57 -0.3161909877759328 1209 58-59 -0.23061803165548156 1209 60-61 -0.2744250366581653 1209 62-63 -0.29894352954719494 1209 64-65 -0.29351879964802663 1209 66-67 -0.21266697604417573 1209 68-69 -0.1283563882403982 1209 70-71 -0.17908334425706585 1209 72-73 -0.22534247106667493 1209 74-75 -0.2331869084229936 1209 76-77 0.0914684181676364 1209 78-79 -0.09127067705227887 1209 80-81 -0.15757797516759808 1209 82-83 -0.15580489748969484 1209 84-85 -0.11103047271658184 1209 86-87 -0.11588046513637096 1209 88-89 -0.11152927567508186 1209 90-91 -0.09722322209472622 1209 92-93 -0.07862143754027073 1209 94-95 -0.07425765636551773 1209 96-97 -0.0693388460996438 1209 98-99 6.38372262717013E-4 1209 100-101 0.10358630361575649 1212 1 -1.0837031896999676 1212 2 0.15226755055520158 1212 3 -1.6142307272135916 1212 4 -0.628398552459899 1212 5 -0.9031565836853233 1212 6 -0.1386419307696869 1212 7 0.006063162439026826 1212 8 0.15398779926546524 1212 9 -0.09312447244617061 1212 10-11 0.14938712313981029 1212 12-13 0.1484610686516845 1212 14-15 0.08697440315929583 1212 16-17 -0.22621350748849522 1212 18-19 -0.08707170152010946 1212 20-21 -0.23580566180949347 1212 22-23 -0.08112256739259038 1212 24-25 -0.1711459602201586 1212 26-27 0.024313708861008365 1212 28-29 -0.0405647250695651 1212 30-31 0.15207527292530898 1212 32-33 0.018356102358076498 1212 34-35 0.09131729462109206 1212 36-37 0.14987438862132763 1212 38-39 0.010671211434399197 1212 40-41 0.042398972893124665 1212 42-43 -0.04029217666835905 1212 44-45 -0.10575595895819134 1212 46-47 -0.2193246854832367 1212 48-49 -0.21079825518906148 1212 50-51 -0.11840528246200677 1212 52-53 -0.00959183953427356 1212 54-55 -0.2745436423924943 1212 56-57 -0.19119492217625833 1212 58-59 0.0070093582741321825 1212 60-61 0.208537410151159 1212 62-63 0.05665637962861325 1212 64-65 0.11990170771884578 1212 66-67 0.106629588895089 1212 68-69 0.07236980532644566 1212 70-71 -0.24477691931996048 1212 72-73 -0.11482135258233228 1212 74-75 -0.042467038916356614 1212 76-77 0.16534505293422086 1212 78-79 0.01400398836371508 1212 80-81 -0.05991842063174957 1212 82-83 -0.04688800050713837 1212 84-85 0.12421524704651787 1212 86-87 0.11333255397473607 1212 88-89 0.09581605811869665 1212 90-91 0.11102747022951576 1212 92-93 0.2034323543994141 1212 94-95 0.05956718495072266 1212 96-97 0.24472239928706685 1212 98-99 0.06866971590934767 1212 100-101 0.38618907926728596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 472.0 3 207.0 4 261.0 5 572.0 6 1348.0 7 3533.0 8 7641.0 9 11294.0 10 14694.0 11 18101.0 12 22807.0 13 28100.0 14 31491.0 15 35284.0 16 39151.0 17 42092.0 18 45605.0 19 49347.0 20 53472.0 21 57917.0 22 65901.0 23 78387.0 24 98155.0 25 127478.0 26 166207.0 27 211478.0 28 269012.0 29 347107.0 30 446607.0 31 578808.0 32 749553.0 33 989701.0 34 1343563.0 35 1863253.0 36 2424302.0 37 3160059.0 38 2525953.0 39 91050.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.836282712526508 25.46324426688937 15.225966248683129 44.474506771901 2 16.8128216776322 29.08454708202024 24.02319598876175 30.079435251585814 3 12.26690625 18.32515 30.3362625 39.07168125 4 16.748702155114387 20.974180852408246 14.465675157465762 47.81144183501161 5 19.57888125 24.9073375 16.801099999999998 38.712681249999996 6 16.06764375 31.2746875 19.67953125 32.978137499999995 7 12.6172 32.80238125 23.67046875 30.909950000000002 8 11.54278125 35.61178125 20.509050000000002 32.3363875 9 11.6114 40.2064375 21.87151875 26.31064375 10-11 11.958490625 37.093321875 23.14003125 27.80815625 12-13 11.144890624999999 40.007156249999994 20.256459375 28.59149375 14-15 11.589390625 37.00291875 20.156809375 31.250881250000003 16-17 11.452621875 38.8853625 20.058009375 29.604006249999998 18-19 14.309596875 32.195918750000004 20.997203125 32.49728125 20-21 16.940596875 33.028240625 21.13981875 28.891343749999997 22-23 10.36123125 36.389953125000005 20.532046875000002 32.71676875 24-25 10.341281450446164 35.8798493643598 20.380302241284525 33.398566943909515 26-27 10.424859374999999 36.404465625 23.7390875 29.4315875 28-29 10.55388223942646 35.96260337149407 19.939829994359062 33.543684394720415 30-31 10.365655866405312 32.832730138218 23.65795493168101 33.14365906369568 32-33 10.407978125 36.414140625 21.723046874999998 31.454834375 34-35 10.52866399244446 35.8600985242611 21.29655422587505 32.3146832574194 36-37 10.473767019871408 35.700798638797785 21.55792565662919 32.26750868470161 38-39 10.430075 36.098990625 21.8443 31.626634375000002 40-41 10.482153125 35.81425625 21.466484375 32.237106250000004 42-43 10.413221875000001 35.47624375 21.810915625 32.29961875 44-45 10.479 36.004284375 21.864728125 31.6519875 46-47 10.527075332127954 35.71328704938952 21.489571237579558 32.27006638090297 48-49 10.483609375 35.4638625 21.829703124999998 32.222825 50-51 10.37559312355892 35.871770967048086 22.02576341957921 31.726872489813786 52-53 10.504565625 35.580084375 21.748175 32.167175 54-55 10.361275000000001 35.35978125 22.023215625 32.255728125 56-57 10.43059375 35.823596875 22.031543749999997 31.714265625 58-59 10.532221875 35.560928125000004 21.774803125000002 32.132046875 60-61 10.438778125 35.402053125 21.989378125 32.169790625 62-63 10.482125 35.830603124999996 22.141128125 31.54614375 64-65 10.4928125 35.715690625 21.707381249999997 32.084115624999995 66-67 10.4288625 35.490740625 21.923684375 32.1567125 68-69 10.501875 35.8758875 22.057225 31.5650125 70-71 10.5956125 35.729853125 21.670662500000002 32.003871875 72-73 10.56369375 35.412896875 21.96005 32.063359375 74-75 10.634940625 35.780812499999996 22.067846875 31.5164 76-77 10.761256249999999 35.589703125 21.7206625 31.928378125000002 78-79 10.813678125 35.386509375 21.9086125 31.891199999999998 80-81 10.971321875 35.76676875 21.999615625 31.262293749999998 82-83 11.199546875 35.580253125 21.598625 31.621575 84-85 11.34434375 35.38599375 21.750228125 31.519434375 86-87 11.612228125 35.742640625 21.797643750000002 30.8474875 88-89 11.984493894346679 35.585282456076534 21.34132370219007 31.088899947386718 90-91 12.282157821580403 35.40828429512515 21.42016809296283 30.889389790331624 92-93 12.712835329674046 35.67557462273204 21.37966646386367 30.231923583730243 94-95 13.25444183433204 35.475493219525475 20.879094024640516 30.39097092150197 96-97 13.709605213694465 35.29265435016905 20.910808339338534 30.08693209679795 98-99 14.314089729967336 35.48038986485382 20.77699091476401 29.428529490414835 100-101 15.159674834065148 35.06182580274918 20.21593937082569 29.562559992359976 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.5 2 3.5 3 3.5 4 2.5 5 5.0 6 5.5 7 4.5 8 9.5 9 12.0 10 11.5 11 11.5 12 17.5 13 25.0 14 31.0 15 42.5 16 54.0 17 73.0 18 110.5 19 146.0 20 223.0 21 380.5 22 616.0 23 1111.0 24 2202.0 25 4563.5 26 9796.5 27 20800.5 28 42510.5 29 81649.5 30 146079.5 31 240190.0 32 361755.0 33 503118.0 34 647519.5 35 775412.5 36 874576.5 37 938172.5 38 961137.5 39 942955.0 40 891943.0 41 821336.0 42 735894.0 43 645850.5 44 564745.0 45 491380.0 46 425720.0 47 373674.5 48 328889.0 49 289485.5 50 266006.0 51 250700.0 52 235469.0 53 226970.0 54 223391.0 55 221014.0 56 220082.5 57 218083.0 58 214684.5 59 209632.0 60 201297.5 61 190557.5 62 179275.0 63 164938.0 64 148358.0 65 132070.0 66 115474.0 67 98760.5 68 82459.5 69 67360.0 70 53616.0 71 41718.5 72 31904.0 73 23895.0 74 17593.0 75 12606.0 76 8854.0 77 6089.5 78 4262.0 79 2935.5 80 1892.0 81 1241.5 82 859.5 83 593.5 84 388.5 85 241.5 86 144.5 87 93.5 88 67.0 89 48.0 90 31.0 91 18.5 92 15.0 93 13.5 94 10.0 95 6.5 96 5.5 97 4.0 98 4.0 99 4.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02945625 2 0.033362499999999996 3 0.0 4 8.75E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.25E-4 26-27 0.0 28-29 9.375000000000001E-6 30-31 0.0012625 32-33 0.0 34-35 5.1875E-4 36-37 1.625E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.1875E-4 48-49 0.0 50-51 4.1562500000000003E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014875 90-91 0.00250625 92-93 0.0055875000000000005 94-95 3.15625E-4 96-97 0.0042093750000000004 98-99 0.0015656250000000002 100-101 2.875E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.815411604985 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13365919235834 60.700481074948634 2 12.995307433134148 19.444985490865047 3 3.794026997610886 8.515550744000516 4 1.1876674115341188 3.5542330497500885 5 0.4568456861810159 1.7089549025797255 6 0.14453733535695498 0.6488172142211002 7 0.06672628068831991 0.34945079081964914 8 0.03545723191484908 0.21221979200662755 9 0.016689942918294103 0.112379845418629 >10 0.13734415340440478 2.661999821139658 >50 0.029803582401974532 1.4314023186927676 >100 0.001668192191396363 0.22323107472105794 >500 8.720635059620557E-5 0.04416538438841135 >1k 1.6257505698774103E-4 0.22034246265875804 >5k 0.0 0.0 >10k+ 1.6778897766491388E-5 0.17178603378931104 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC 17277 0.10798125 TruSeq Adapter, Index 12 (100% over 50bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.4375E-4 0.0 0.0 2 7.75E-4 0.0 0.0 3 7.9375E-4 1.25E-5 6.25E-6 4 8.75E-4 1.25E-5 6.25E-6 5 9.25E-4 1.25E-5 6.25E-6 6 0.00103125 1.25E-5 6.25E-6 7 0.00119375 1.25E-5 6.25E-6 8 0.00124375 1.25E-5 6.25E-6 9 0.00128125 1.875E-5 6.25E-6 10-11 0.00146875 1.875E-5 6.25E-6 12-13 0.00238125 1.875E-5 6.25E-6 14-15 0.00379375 1.875E-5 6.25E-6 16-17 0.005228125 3.75E-5 6.25E-6 18-19 0.005865625 5.3125000000000004E-5 6.25E-6 20-21 0.006565625 6.875E-5 6.25E-6 22-23 0.015503125 6.875E-5 6.25E-6 24-25 0.0374375 6.875E-5 6.25E-6 26-27 0.042003125 6.875E-5 6.25E-6 28-29 0.043946874999999996 6.875E-5 6.25E-6 30-31 0.045649999999999996 6.875E-5 6.25E-6 32-33 0.047878125 7.500000000000001E-5 6.25E-6 34-35 0.050528125 8.75E-5 6.25E-6 36-37 0.053978125 8.75E-5 6.25E-6 38-39 0.0581125 8.75E-5 6.25E-6 40-41 0.0632875 9.0625E-5 6.25E-6 42-43 0.070015625 9.687500000000001E-5 9.375000000000001E-6 44-45 0.078325 1.0E-4 1.25E-5 46-47 0.08871875 1.0E-4 1.25E-5 48-49 0.10254374999999999 1.0625E-4 1.25E-5 50-51 0.120003125 1.0625E-4 1.25E-5 52-53 0.14150625 1.0625E-4 1.25E-5 54-55 0.16915 1.0625E-4 1.5625E-5 56-57 0.20400000000000001 1.0625E-4 2.5E-5 58-59 0.249809375 1.0625E-4 2.5E-5 60-61 0.30779687499999997 1.0625E-4 2.5E-5 62-63 0.38273124999999997 1.0625E-4 2.5E-5 64-65 0.47791562499999996 1.0625E-4 3.125E-5 66-67 0.5983875000000001 1.0625E-4 3.125E-5 68-69 0.75054375 1.0625E-4 3.125E-5 70-71 0.944671875 1.125E-4 3.125E-5 72-73 1.19034375 1.1875E-4 3.4374999999999995E-5 74-75 1.4958437500000001 1.1875E-4 4.375E-5 76-77 1.8770625 1.1875E-4 4.375E-5 78-79 2.352103125 1.1875E-4 4.375E-5 80-81 2.94125 1.1875E-4 4.9999999999999996E-5 82-83 3.6637062499999997 1.1875E-4 5.625E-5 84-85 4.542521875 1.1875E-4 5.625E-5 86-87 5.601775 1.1875E-4 5.625E-5 88-89 6.860496875000001 1.28125E-4 5.625E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATAAAT 131315 0.0 72.6866 1 ATAAATC 138185 0.0 69.94685 2 TAAATCA 150325 0.0 64.711395 3 TCATAAA 168210 0.0 58.841843 7 AATCATA 171615 0.0 57.76847 5 ATCATAA 184750 0.0 54.144707 6 AAATCAT 180675 0.0 53.946266 4 CATAAAC 212555 0.0 46.755714 8 ATAAACA 228385 0.0 43.506626 9 GTCCCCA 3455 0.0 33.016037 1 ACAAACG 175695 0.0 29.231344 12-13 AAACGGG 184290 0.0 27.517515 14-15 CAAACGG 181765 0.0 26.892387 14-15 TGATACG 2810 0.0 26.87651 3 CCCCGCA 5575 0.0 26.087849 1 CCCCCGA 5100 0.0 25.628563 1 ACGGGCA 199975 0.0 25.376997 16-17 GGGCAAC 205575 0.0 24.4086 18-19 AACGGGC 196925 0.0 24.404837 16-17 CGGGCAA 205240 0.0 23.249624 18-19 >>END_MODULE