##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2212_lane2_CTTGTA_L002_R1_003.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16000000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.1404206875	31.0	26.0	33.0	16.0	34.0
2	29.1095369375	31.0	28.0	34.0	16.0	34.0
3	30.1515914375	31.0	30.0	34.0	25.0	34.0
4	33.294161125	35.0	33.0	37.0	26.0	37.0
5	33.3299579375	35.0	33.0	37.0	26.0	37.0
6	34.570853375	35.0	35.0	37.0	31.0	37.0
7	34.9365723125	36.0	35.0	37.0	32.0	37.0
8	35.052157	37.0	35.0	37.0	32.0	37.0
9	36.74283525	39.0	37.0	39.0	32.0	39.0
10-11	36.77679103125	39.0	37.0	39.0	32.0	39.0
12-13	36.881129093750005	39.0	37.0	39.0	33.0	39.0
14-15	38.0471919375	40.0	37.0	41.0	33.0	41.0
16-17	38.027122343749994	40.0	37.0	41.0	33.0	41.0
18-19	38.19548303125	40.0	37.5	41.0	33.5	41.0
20-21	38.44876549999999	40.0	38.0	41.0	34.0	41.0
22-23	38.4199083125	40.0	38.0	41.0	34.0	41.0
24-25	38.329437687500004	40.0	38.0	41.0	34.0	41.0
26-27	38.1858130625	40.0	38.0	41.0	33.5	41.0
28-29	38.00366815625	40.0	37.0	41.0	33.0	41.0
30-31	37.95376546875	40.0	37.0	41.0	33.0	41.0
32-33	37.7878538125	40.0	37.0	41.0	33.0	41.0
34-35	37.7106938125	40.0	37.0	41.0	32.0	41.0
36-37	37.62949340625	40.0	37.0	41.0	32.0	41.0
38-39	37.522431312500004	40.0	37.0	41.0	32.0	41.0
40-41	37.40804053125	40.0	36.5	41.0	31.5	41.0
42-43	37.285602781250006	40.0	36.0	41.0	31.0	41.0
44-45	37.128042031250004	40.0	36.0	41.0	31.0	41.0
46-47	36.97733546875	39.5	35.0	41.0	31.0	41.0
48-49	36.83554809375	39.0	35.0	41.0	30.5	41.0
50-51	36.67042946875	39.0	35.0	41.0	30.0	41.0
52-53	36.50210196875	39.0	35.0	41.0	30.0	41.0
54-55	36.2929985	39.0	35.0	41.0	30.0	41.0
56-57	36.062907531250005	38.5	35.0	41.0	29.0	41.0
58-59	35.89205628125	38.0	34.0	40.0	29.0	41.0
60-61	35.622134875	37.5	34.0	40.0	29.0	41.0
62-63	35.29499109375	37.0	34.0	40.0	28.0	41.0
64-65	34.957755593749994	36.5	34.0	39.0	28.0	41.0
66-67	34.530589343749995	36.0	34.0	39.0	28.0	40.5
68-69	34.10636459375	35.5	33.0	38.5	28.0	40.0
70-71	33.68914253125	35.0	33.0	37.0	27.5	39.0
72-73	33.286032875000004	35.0	33.0	37.0	26.5	39.0
74-75	32.88495025	35.0	33.0	36.0	26.0	38.5
76-77	30.7755084375	33.0	30.0	34.5	23.5	36.5
78-79	32.087417875	34.5	32.0	35.0	26.0	37.0
80-81	32.144031625	35.0	32.5	35.0	26.0	36.0
82-83	31.97927090625	35.0	32.5	35.0	26.0	36.0
84-85	31.81246040625	35.0	32.0	35.0	26.0	36.0
86-87	31.66668146875	35.0	32.0	35.0	25.5	35.0
88-89	31.47523696875	34.5	32.0	35.0	25.0	35.0
90-91	31.33580390625	34.0	32.0	35.0	25.0	35.0
92-93	31.1380130625	34.0	32.0	35.0	24.0	35.0
94-95	30.96841246875	34.0	32.0	35.0	23.5	35.0
96-97	30.714756375	34.0	32.0	35.0	22.5	35.0
98-99	30.43827940625	34.0	32.0	35.0	19.5	35.0
100-101	29.29381896875	33.5	29.0	35.0	9.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	2.3278731857702724
2201	2	2.360915235023338
2201	3	1.7506663885892912
2201	4	0.6398073976564334
2201	5	1.930155326252084
2201	6	0.9107333419737955
2201	7	0.5041886398490263
2201	8	0.33015416121534713
2201	9	0.22431246628055135
2201	10-11	-0.01734772089177028
2201	12-13	0.0024592238329645966
2201	14-15	-0.10153073275061786
2201	16-17	-0.10832994271322605
2201	18-19	0.055932155733117384
2201	20-21	0.17956499143959093
2201	22-23	0.16590339632127638
2201	24-25	0.1700006256461819
2201	26-27	0.11921978429172952
2201	28-29	0.2728594531230826
2201	30-31	0.3272813063379445
2201	32-33	0.29835353133535136
2201	34-35	0.2234773852567855
2201	36-37	0.26489908652718697
2201	38-39	0.3322139900058261
2201	40-41	0.3913287366456828
2201	42-43	0.3208517170043095
2201	44-45	0.3133744739269346
2201	46-47	0.33606875268394276
2201	48-49	0.3595076848686318
2201	50-51	0.3238133647362247
2201	52-53	0.38686859824000663
2201	54-55	0.4191025528733263
2201	56-57	0.3451224363058145
2201	58-59	0.37131675031152156
2201	60-61	0.3739568986733417
2201	62-63	0.35913481937021885
2201	64-65	0.3186521289088091
2201	66-67	0.29233434624332943
2201	68-69	0.35374780419233787
2201	70-71	0.439843854257731
2201	72-73	0.4041977719304981
2201	74-75	0.33031181497766227
2201	76-77	0.19861054108525877
2201	78-79	0.3116448137890231
2201	80-81	0.4336941735436568
2201	82-83	0.3401229447249925
2201	84-85	0.411611849636607
2201	86-87	0.37773702584151536
2201	88-89	0.3652727440151615
2201	90-91	0.34887113661127955
2201	92-93	0.24801863318619155
2201	94-95	0.16193050625438588
2201	96-97	0.2527239049160954
2201	98-99	0.2640469605374136
2201	100-101	0.2063845351238065
2202	1	1.935514350612742
2202	2	1.589473470656717
2202	3	0.5329478137603836
2202	4	0.6640159285300271
2202	5	0.7680660467911338
2202	6	0.48985354017524685
2202	7	0.29095412753853367
2202	8	0.21452768429609392
2202	9	0.17984962032900853
2202	10-11	0.10743479537752876
2202	12-13	0.11341833444446792
2202	14-15	0.15199763466785043
2202	16-17	0.1277021826368454
2202	18-19	0.18365762258589768
2202	20-21	0.21457997404620954
2202	22-23	0.17380993493785724
2202	24-25	0.2390586658288072
2202	26-27	0.21330561440698403
2202	28-29	0.20561521962941498
2202	30-31	0.1641569731349577
2202	32-33	0.090555504944561
2202	34-35	0.1905351079871167
2202	36-37	0.17422658239350142
2202	38-39	0.18536454006614633
2202	40-41	0.16761498680093467
2202	42-43	0.21470663169153426
2202	44-45	0.1910551389006585
2202	46-47	0.23127333616886858
2202	48-49	0.24020584905024123
2202	50-51	0.1941760067323841
2202	52-53	0.2551117114827761
2202	54-55	0.2843254892578102
2202	56-57	0.1910656248277931
2202	58-59	0.24216438395304607
2202	60-61	0.24858717012467935
2202	62-63	0.2910493736308055
2202	64-65	0.26906807263942056
2202	66-67	0.2655124197125218
2202	68-69	0.24433565733452411
2202	70-71	0.29209199180115775
2202	72-73	0.27652669595977386
2202	74-75	0.22190513914480903
2202	76-77	0.1568400593117616
2202	78-79	0.2603376229890202
2202	80-81	0.3088124645132524
2202	82-83	0.3020478864958527
2202	84-85	0.30489145517579175
2202	86-87	0.2835495070757936
2202	88-89	0.3103598930239606
2202	90-91	0.3280597372541578
2202	92-93	0.28766549436636524
2202	94-95	0.31089724175026134
2202	96-97	0.2945016881634537
2202	98-99	0.31106978213404446
2202	100-101	0.3028223885248913
2203	1	2.394114650572927
2203	2	2.351343716693709
2203	3	1.5142578662856678
2203	4	1.446753772943211
2203	5	2.114045434957646
2203	6	1.0161881527963388
2203	7	0.44184375226763706
2203	8	0.24325860201549432
2203	9	0.19273877773512282
2203	10-11	0.09896504571180742
2203	12-13	0.10079659190709123
2203	14-15	0.02009818017351961
2203	16-17	0.031925307804968384
2203	18-19	0.12852860593873316
2203	20-21	0.1604787350267145
2203	22-23	0.16908282352094517
2203	24-25	0.15653196005666814
2203	26-27	0.1336965012498723
2203	28-29	0.11848006680900625
2203	30-31	0.1464523750808766
2203	32-33	0.144600925501571
2203	34-35	0.193823609270666
2203	36-37	0.11226318275186742
2203	38-39	0.15194118821369784
2203	40-41	0.20230950136101455
2203	42-43	0.15891809713483696
2203	44-45	0.1605224421099578
2203	46-47	0.17857487035470143
2203	48-49	0.16178812269742338
2203	50-51	0.19053162784572208
2203	52-53	0.14796868943472674
2203	54-55	0.13579848756016588
2203	56-57	0.21884177957809925
2203	58-59	0.16565286037899796
2203	60-61	0.1624971114771654
2203	62-63	0.1366351798352099
2203	64-65	0.07368004549059037
2203	66-67	0.12904109115468998
2203	68-69	0.0963476606703324
2203	70-71	0.06685064141785801
2203	72-73	0.0948097401263297
2203	74-75	0.1286426435729382
2203	76-77	-0.06675990175077828
2203	78-79	0.03684040386143295
2203	80-81	0.03448283096898308
2203	82-83	0.022599092610622762
2203	84-85	0.0326027992942457
2203	86-87	-0.05268397370877054
2203	88-89	0.01569477542743769
2203	90-91	0.011305130643041394
2203	92-93	-0.019342534923097787
2203	94-95	-0.018591510380687737
2203	96-97	-0.04209462105059458
2203	98-99	-7.210829940333952E-4
2203	100-101	0.047168161902099826
2204	1	1.760315335339719
2204	2	1.5245435853932996
2204	3	1.0549563662288044
2204	4	1.5057570815169399
2204	5	0.020156490942028427
2204	6	0.24395912259092256
2204	7	0.176151232212149
2204	8	0.10163274461028493
2204	9	0.11390993641905567
2204	10-11	0.1421142551051986
2204	12-13	0.04743307448276113
2204	14-15	0.04442298336999784
2204	16-17	0.09615221690798137
2204	18-19	0.0918910177505694
2204	20-21	0.13580002881374753
2204	22-23	0.056674422156206106
2204	24-25	0.0678187524430598
2204	26-27	0.07853300234170035
2204	28-29	0.12039808336303537
2204	30-31	0.13066068975000888
2204	32-33	0.16718268687875337
2204	34-35	0.14438104792513684
2204	36-37	0.12445788460625096
2204	38-39	0.10262637617701387
2204	40-41	0.10824821191459932
2204	42-43	0.07474799895683049
2204	44-45	0.16571788401636667
2204	46-47	0.18147831079067345
2204	48-49	0.1653610553365894
2204	50-51	0.07326852233524761
2204	52-53	0.13801549829616278
2204	54-55	0.2328027433216775
2204	56-57	0.2597625569684112
2204	58-59	0.2159114357261558
2204	60-61	0.28068706145887745
2204	62-63	0.2431383863756409
2204	64-65	0.19270434222203647
2204	66-67	0.17229270934912222
2204	68-69	0.14222041284612175
2204	70-71	0.20451747839500456
2204	72-73	0.2280109407954356
2204	74-75	0.26967453691351295
2204	76-77	0.06738153604403507
2204	78-79	0.19911032277880025
2204	80-81	0.21147121404105462
2204	82-83	0.24330172066354905
2204	84-85	0.20631502995571083
2204	86-87	0.24221443494430517
2204	88-89	0.25591092173396035
2204	90-91	0.24579108005710637
2204	92-93	0.30472849230926613
2204	94-95	0.3390780137772005
2204	96-97	0.34717596112430016
2204	98-99	0.3403243171575987
2204	100-101	0.3823370025084394
2205	1	-1.470451308894308
2205	2	0.39677211021608016
2205	3	0.7729419639610491
2205	4	-0.07499879967426892
2205	5	-1.2509987020247877
2205	6	-0.4190905385109147
2205	7	-0.19459303599593625
2205	8	-0.08887584103095492
2205	9	-0.05420223347694986
2205	10-11	-0.00778465064952627
2205	12-13	0.022164930338995248
2205	14-15	-0.02296409412798539
2205	16-17	-0.023550063485899386
2205	18-19	0.035783867107817
2205	20-21	-0.017821706397278092
2205	22-23	-0.04055969347291466
2205	24-25	0.03691018369117671
2205	26-27	-0.01187011494123169
2205	28-29	-0.033064472628190344
2205	30-31	0.03948254109015892
2205	32-33	-0.08603816565585731
2205	34-35	-0.08530761067493131
2205	36-37	-0.10108072593238404
2205	38-39	-0.0844477585260961
2205	40-41	-0.0215977153251643
2205	42-43	-0.07228460160383321
2205	44-45	-0.04638783769613042
2205	46-47	-0.04675683138276554
2205	48-49	-0.05415886321236485
2205	50-51	-0.017751082612122104
2205	52-53	0.014548029755694358
2205	54-55	0.012128188445856836
2205	56-57	0.033727910069551115
2205	58-59	0.04712378972959641
2205	60-61	0.02415191919013182
2205	62-63	-0.0014491222200874176
2205	64-65	-0.023607366109445138
2205	66-67	0.032493390570763836
2205	68-69	-0.012682223479174581
2205	70-71	-9.025749073998668E-5
2205	72-73	0.05465803656537105
2205	74-75	0.06060669473230007
2205	76-77	0.027054277765653723
2205	78-79	0.02452460856076044
2205	80-81	0.03409393416204409
2205	82-83	0.0942327980155504
2205	84-85	0.0922798486648837
2205	86-87	0.08030226526951978
2205	88-89	0.12286290026650803
2205	90-91	0.10812873631581255
2205	92-93	0.14336965309249194
2205	94-95	0.13265944298309051
2205	96-97	0.12942394914419708
2205	98-99	0.1071369773204296
2205	100-101	0.10985312989506824
2206	1	-1.304504283543654
2206	2	-1.355394161926263
2206	3	-1.4822248758121788
2206	4	-0.691681914489493
2206	5	-0.9888613754080495
2206	6	-0.4353058727560537
2206	7	-0.1725416909823565
2206	8	-0.050296053353847014
2206	9	0.041090708942491005
2206	10-11	0.08533480132312121
2206	12-13	0.10544595320450867
2206	14-15	0.0759608334900932
2206	16-17	0.052999819173408014
2206	18-19	0.005843466932198282
2206	20-21	-0.12017855214876505
2206	22-23	-0.10725889016850942
2206	24-25	-0.057681849110849726
2206	26-27	-0.08163923555678565
2206	28-29	-0.09851813571234658
2206	30-31	-0.08344946275097698
2206	32-33	-0.10869919015519258
2206	34-35	-0.07779849360588287
2206	36-37	-0.1042264888926141
2206	38-39	-0.1163948243456332
2206	40-41	-0.059804725240297785
2206	42-43	-0.0492784035046725
2206	44-45	-0.09313772059223169
2206	46-47	-0.1346139133870281
2206	48-49	-0.1771683679989593
2206	50-51	-0.16311586317082316
2206	52-53	-0.11190922847411144
2206	54-55	-0.7152761715519063
2206	56-57	-0.6159596940716554
2206	58-59	-0.23569472668093283
2206	60-61	-0.17686059373782825
2206	62-63	-0.11730144808355902
2206	64-65	-0.0861850403636808
2206	66-67	-0.07869007022112129
2206	68-69	-0.1059523464578973
2206	70-71	-0.03942916623144299
2206	72-73	-0.08092952162662925
2206	74-75	-0.08672283618606969
2206	76-77	-0.15419272749047863
2206	78-79	-0.060844690158980086
2206	80-81	-0.021339092971416562
2206	82-83	-0.06476824515407387
2206	84-85	-0.04697736669686492
2206	86-87	-0.008765085373465098
2206	88-89	-0.058409969459429334
2206	90-91	-0.052157618315344934
2206	92-93	-0.027193286055680943
2206	94-95	0.01683977622301569
2206	96-97	0.10240492977667515
2206	98-99	0.14147303357233199
2206	100-101	0.17295624749712246
2207	1	-2.0674093231502226
2207	2	-2.655457647275334
2207	3	-2.174028970460398
2207	4	-2.025621610115447
2207	5	-1.9822685660485249
2207	6	-0.9553129826040134
2207	7	-0.47344712137566347
2207	8	-0.21275445250953595
2207	9	-0.11111536974248537
2207	10-11	-0.030403116290429466
2207	12-13	-0.14764250482458152
2207	14-15	-0.12653127875493908
2207	16-17	-0.190630151090474
2207	18-19	-0.2723727298585459
2207	20-21	-0.4196405909321541
2207	22-23	-0.25960927031118075
2207	24-25	-0.23005714769949748
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2215	76-77	-0.22519348430294883
2215	78-79	-0.11034321957109228
2215	80-81	-0.10068540989735197
2215	82-83	-0.055891347548261194
2215	84-85	-0.15432278573058156
2215	86-87	-0.11707100420229821
2215	88-89	-0.1021305077889032
2215	90-91	-0.09497385108030088
2215	92-93	-0.05468039532331659
2215	94-95	-0.024292852396762044
2215	96-97	-0.04519869481654837
2215	98-99	-0.03125269526474028
2215	100-101	0.023168334583569816
2214	1	-2.349291899322438
2214	2	-3.669166654932816
2214	3	-1.2229989952205784
2214	4	-1.2209238615008786
2214	5	-2.2155114655627948
2214	6	-1.0383767553332461
2214	7	-0.4116249144059694
2214	8	-0.21403021774609954
2214	9	-0.2433527872189103
2214	10-11	-0.058929712117574695
2214	12-13	-0.0856770158839808
2214	14-15	-0.1523080783409796
2214	16-17	-0.11481528674202224
2214	18-19	-0.08941032006335092
2214	20-21	-0.1554977627361609
2214	22-23	-0.2823304419899202
2214	24-25	-0.24162925472395358
2214	26-27	-0.26989259281837263
2214	28-29	-0.37795965867150727
2214	30-31	-0.3354265532872702
2214	32-33	-0.2948785838098331
2214	34-35	-0.2692115774485089
2214	36-37	-0.31535902224231194
2214	38-39	-0.29900947316787096
2214	40-41	-0.3489245929368252
2214	42-43	-0.32899714486977416
2214	44-45	-0.38142916723166564
2214	46-47	-0.3376061313914249
2214	48-49	-0.35125344746558085
2214	50-51	-0.4018400988885915
2214	52-53	-0.4407426538484671
2214	54-55	-0.34976461453988605
2214	56-57	-0.3994601844195955
2214	58-59	-0.39147727199623006
2214	60-61	-0.44489836414028616
2214	62-63	-0.37884182604300776
2214	64-65	-0.341961619206522
2214	66-67	-0.33899263587149164
2214	68-69	-0.3282198623992798
2214	70-71	-0.29163700681151283
2214	72-73	-0.23158593384257387
2214	74-75	-0.28802018182305034
2214	76-77	-0.3293008581835153
2214	78-79	-0.3242839660198733
2214	80-81	-0.3106302143980457
2214	82-83	-0.2979881115023808
2214	84-85	-0.24878528314511072
2214	86-87	-0.2579431312171394
2214	88-89	-0.2388933034043994
2214	90-91	-0.3035067184534306
2214	92-93	-0.2647624388613501
2214	94-95	-0.2746091437268845
2214	96-97	-0.33730218801845524
2214	98-99	-0.3080845507037644
2214	100-101	-0.21366279978780867
2213	1	-3.622186971389972
2213	2	-4.151034634833312
2213	3	-1.5965595064073845
2213	4	-1.8523301878228082
2213	5	-2.2975378211244113
2213	6	-1.032432687676895
2213	7	-0.44663031642073037
2213	8	-0.27351753409868707
2213	9	-0.1607240225833806
2213	10-11	-0.057909172858288116
2213	12-13	-0.14071706494073766
2213	14-15	-0.12161629206027413
2213	16-17	-0.1777709262740501
2213	18-19	-0.2003893649747539
2213	20-21	-0.2422953438550266
2213	22-23	-0.23038510106487564
2213	24-25	-0.3657088632202914
2213	26-27	-0.35245131287702236
2213	28-29	-0.4840198115969727
2213	30-31	-0.43773906344925706
2213	32-33	-0.34793761243207655
2213	34-35	-0.34299485443816735
2213	36-37	-0.33482264806058737
2213	38-39	-0.3364104160979764
2213	40-41	-0.3452106298851163
2213	42-43	-0.34725855627842606
2213	44-45	-0.44073364865482034
2213	46-47	-0.4219219752616965
2213	48-49	-0.35850632189993803
2213	50-51	-0.3991168914589096
2213	52-53	-0.39193824578163117
2213	54-55	-0.36003381069910745
2213	56-57	-0.3654005318220328
2213	58-59	-0.39185797930630883
2213	60-61	-0.40532329594631733
2213	62-63	-0.35724776886207366
2213	64-65	-0.31564718498145794
2213	66-67	-0.34383671998034515
2213	68-69	-0.3490841634639992
2213	70-71	-0.32913647119143974
2213	72-73	-0.2835840214641152
2213	74-75	-0.31787938996399134
2213	76-77	-0.29111493211340544
2213	78-79	-0.24557335859825002
2213	80-81	-0.27726896961467773
2213	82-83	-0.21564953820439925
2213	84-85	-0.1745652085893532
2213	86-87	-0.1883040712332864
2213	88-89	-0.19171265614734878
2213	90-91	-0.1838536162667488
2213	92-93	-0.20260122572228312
2213	94-95	-0.1676718690544874
2213	96-97	-0.179608620896758
2213	98-99	-0.11466007861854877
2213	100-101	-0.03017760967164307
2212	1	-3.0958440241947365
2212	2	-2.0958830095073395
2212	3	-0.5986713532953587
2212	4	-3.142005599119699
2212	5	-3.06284310128407
2212	6	-1.4271722893260304
2212	7	-0.7828898550015282
2212	8	-0.377522528744727
2212	9	-0.2519608644725082
2212	10-11	-0.1898976194231352
2212	12-13	-0.19036750248725554
2212	14-15	-0.2328973298055672
2212	16-17	-0.37958040231889356
2212	18-19	-0.38630130625115555
2212	20-21	-0.5518137391041051
2212	22-23	-0.43906660694221245
2212	24-25	-0.4899435976084021
2212	26-27	-0.5155225930594654
2212	28-29	-0.49919801076706705
2212	30-31	-0.6353855757607434
2212	32-33	-0.5495681197486633
2212	34-35	-0.5662075286579551
2212	36-37	-0.48928312842983956
2212	38-39	-0.532490126024399
2212	40-41	-0.42947695271543296
2212	42-43	-0.46576688610902295
2212	44-45	-0.4661280547989932
2212	46-47	-0.42412761727061365
2212	48-49	-0.5427182167473745
2212	50-51	-0.44496241514666934
2212	52-53	-0.43794543852784074
2212	54-55	-0.4135980524602303
2212	56-57	-0.41813948957273794
2212	58-59	-0.48707445443654507
2212	60-61	-0.4737286282673878
2212	62-63	-0.46363451408637957
2212	64-65	-0.48669044731667555
2212	66-67	-0.5072481813413248
2212	68-69	-0.40913069310809647
2212	70-71	-0.49576824143420595
2212	72-73	-0.4492225738583002
2212	74-75	-0.4105210860129631
2212	76-77	-0.39689433529682105
2212	78-79	-0.4069028733925961
2212	80-81	-0.41726719624102415
2212	82-83	-0.3823186433898904
2212	84-85	-0.3885506128375411
2212	86-87	-0.3780703998636774
2212	88-89	-0.3345283438955171
2212	90-91	-0.38086502100369657
2212	92-93	-0.338627105186756
2212	94-95	-0.2590159104042762
2212	96-97	-0.18183721237910078
2212	98-99	-0.1554834976446955
2212	100-101	-0.09303120739983228
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1621.0
3	215.0
4	337.0
5	671.0
6	1493.0
7	3704.0
8	7690.0
9	11818.0
10	15639.0
11	19088.0
12	23270.0
13	28580.0
14	32185.0
15	35817.0
16	38696.0
17	41120.0
18	44169.0
19	47138.0
20	50629.0
21	54619.0
22	61562.0
23	73093.0
24	89966.0
25	115883.0
26	150094.0
27	191726.0
28	240810.0
29	309106.0
30	397917.0
31	515166.0
32	671062.0
33	896045.0
34	1240165.0
35	1767715.0
36	2383449.0
37	3206008.0
38	3076849.0
39	154790.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.508548584486416	25.562692744735028	15.82050660222987	44.10825206854869
2	16.655266313255712	29.192542468816534	24.314560001360043	29.83763121656771
3	11.877313050633708	18.496236306384	30.92531900364338	38.70113163933892
4	17.00124320482717	21.215808195326723	14.291374186027717	47.49157441381839
5	19.909377432033075	24.983193737395304	16.481974861481145	38.62545396909048
6	16.138875	31.49173125	19.520993750000002	32.8484
7	12.622800000000002	32.700275	23.862762500000002	30.8141625
8	11.5557125	35.52235	20.429918750000002	32.49201875
9	11.60448125	40.4613375	21.765825	26.168356250000002
10-11	11.972790625	37.115453124999995	23.195009375	27.716746875
12-13	11.13270625	40.1850375	20.17791875	28.5043375
14-15	11.585090625	37.06080625	20.04969375	31.304409375
16-17	11.415663627638045	39.023899998743744	20.010760652574174	29.549675721044032
18-19	14.440190625	32.086259375	20.91449375	32.559056250000005
20-21	17.195856010298506	32.89611747606241	21.069195568010144	28.838830945628942
22-23	10.306334374999999	36.443471875	20.455571875	32.794621875000004
24-25	10.28945162877343	35.9242605878734	20.29587078976743	33.49041699358575
26-27	10.387386130895317	36.422844543759666	23.80107845820808	29.388690867136937
28-29	10.497251726719755	35.96812167372244	19.879850174129025	33.65477642542877
30-31	10.319757771168232	32.71409290615877	23.734416575675432	33.23173274699757
32-33	10.35669950721059	36.412659849056915	21.7124091355854	31.518231508147093
34-35	10.48783888742256	35.88045557554666	21.256320276641848	32.37538526038893
36-37	10.42351235967065	35.730381392069646	21.532793542763905	32.3133127054958
38-39	10.3782902734394	36.115813900058015	21.820029947571125	31.685865878931462
40-41	10.449089413351103	35.82592798900624	21.43860424890957	32.28637834873309
42-43	10.3806967788162	35.49545713342039	21.78030606657121	32.343540021192204
44-45	10.424680863882987	36.021492137089325	21.822259150020773	31.731567849006915
46-47	10.490646245588811	35.73459561314578	21.44928177538677	32.32547636587865
48-49	10.440536822767573	35.492023265478295	21.790151315859788	32.27728859589435
50-51	10.329026410850917	35.893234589389024	21.983367272084998	31.794371727675063
52-53	10.47322478907169	35.571606125155355	21.74083464823032	32.21433443754263
54-55	10.33034275527661	35.391498329137754	21.9988690605562	32.27928985502943
56-57	10.39287844977745	35.82495111952972	21.994119437316233	31.788050993376594
58-59	10.495209375	35.59196875	21.737000000000002	32.175821875
60-61	10.396346334814455	35.448591083206686	21.953603744665564	32.20145883731329
62-63	10.44111531640187	35.87076315896958	22.091444349821842	31.596677174806704
64-65	10.448111334020876	35.74139420151141	21.6729071886701	32.137587275797614
66-67	10.386959375	35.535521875	21.896887500000002	32.180631250000005
68-69	10.449856952901488	35.947181167470575	21.99263123848631	31.610330641141626
70-71	10.531003125	35.782965625	21.6361625	32.04986875
72-73	10.521553722211928	35.45327002492337	21.923055299458376	32.10212095340633
74-75	10.586024367952673	35.82082355387507	22.02392020124564	31.569231876926608
76-77	10.73803902227156	35.63919360351534	21.667354559174136	31.955412815038958
78-79	10.786900381227001	35.42988913191794	21.872921082835685	31.91028940401937
80-81	10.933490325264923	35.80742917503673	21.92302762010911	31.336052879589232
82-83	11.166827026878547	35.60476081234549	21.552709353201937	31.675702807574023
84-85	11.306272177107795	35.44250932710032	21.698411178051405	31.552807317740484
86-87	11.583315625	35.79700625	21.724712500000003	30.894965624999998
88-89	11.938359162069858	35.61072783333149	21.305345869545413	31.14556713505324
90-91	12.241720952891653	35.442212792293326	21.38668247879633	30.929383776018692
92-93	12.678064138439963	35.747742639844745	21.326007845789693	30.248185375925594
94-95	13.22287225471829	35.53065769007638	20.834983901414176	30.411486153791152
96-97	13.675428824076869	35.329557564536316	20.861358546126198	30.133655065260623
98-99	14.282915625000001	35.54366875	20.719737499999997	29.453678125
100-101	15.12181875	35.1488125	20.183659374999998	29.545709375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	3.5
2	1.0
3	2.0
4	2.5
5	2.0
6	4.5
7	10.5
8	14.5
9	24.0
10	41.5
11	56.5
12	65.5
13	90.5
14	121.5
15	159.5
16	207.0
17	258.0
18	305.0
19	405.0
20	559.5
21	725.5
22	1020.5
23	1626.0
24	2884.0
25	5545.5
26	10922.0
27	22263.5
28	44595.5
29	84746.0
30	150100.0
31	244873.5
32	367638.5
33	509046.0
34	653364.0
35	781644.5
36	879640.5
37	940634.5
38	960629.5
39	940544.5
40	888990.5
41	816102.5
42	728358.0
43	637052.0
44	553847.5
45	479674.0
46	415423.0
47	364088.5
48	320409.0
49	284990.0
50	264462.5
51	250305.5
52	235801.0
53	227473.5
54	224698.0
55	223506.0
56	222256.0
57	220235.0
58	217376.0
59	212273.5
60	204159.5
61	193667.0
62	181564.5
63	166955.0
64	150321.0
65	133216.5
66	116127.5
67	99014.5
68	82683.0
69	67747.0
70	54227.5
71	42082.5
72	31842.5
73	23861.5
74	17652.0
75	12609.0
76	8895.5
77	6303.5
78	4537.0
79	3126.5
80	2046.5
81	1461.5
82	1062.5
83	733.0
84	478.0
85	353.0
86	262.0
87	195.0
88	146.5
89	111.5
90	87.0
91	75.0
92	69.0
93	50.5
94	42.0
95	37.5
96	30.5
97	24.5
98	15.5
99	8.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005375
2	0.0031000000000000003
3	1.625E-4
4	0.00139375
5	7.500000000000001E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	2.5625E-4
18-19	0.0
20-21	5.3125E-5
22-23	0.0
24-25	0.001534375
26-27	0.001340625
28-29	0.001415625
30-31	0.004678125
32-33	3.8750000000000004E-4
34-35	6.6875E-4
36-37	0.00365625
38-39	2.3750000000000003E-4
40-41	7.8125E-5
42-43	0.001534375
44-45	5.625E-5
46-47	3.8124999999999997E-4
48-49	9.8125E-4
50-51	6.187499999999999E-4
52-53	8.75E-5
54-55	1.7187500000000002E-4
56-57	3.1249999999999997E-6
58-59	0.0
60-61	4.1562500000000003E-4
62-63	0.001134375
64-65	1.8750000000000002E-5
66-67	0.0
68-69	1.5625E-4
70-71	0.0
72-73	0.004728125
74-75	3.1875E-4
76-77	0.0040593750000000005
78-79	5.25E-4
80-81	1.6874999999999998E-4
82-83	5.21875E-4
84-85	0.001578125
86-87	0.0
88-89	0.001359375
90-91	0.002928125
92-93	0.01803125
94-95	0.0028625
96-97	0.002153125
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.25423165715243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4045065302281	60.50779360704811
2	13.909458796621	20.934912690130666
3	3.7233775648985725	8.40599753447764
4	1.1065182994175053	3.330807377489733
5	0.42447897270107526	1.59719194726184
6	0.124390254466303	0.5616535815299584
7	0.054508440428723416	0.28713935623052717
8	0.05185526290083061	0.31218623735853196
9	0.023974662982601404	0.16237753577953587
>10	0.16690994053105215	2.861222337228533
>50	0.00830274046419462	0.3926660058640497
>100	0.0013803732163187523	0.21278592745474167
>500	2.0720164963456788E-4	0.10274504338949582
>1k	1.1428536484481515E-4	0.16532861363345247
>5k	8.363694367948068E-6	0.06289628110997524
>10k+	8.310434864083558E-6	0.10229592401319453
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	16357	0.10223125	TruSeq Adapter, Index 12 (100% over 50bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.375E-4	0.0	0.0
2	5.9375E-4	0.0	0.0
3	6.1875E-4	1.25E-5	0.0
4	6.375E-4	1.25E-5	0.0
5	6.75E-4	1.25E-5	0.0
6	8.25E-4	1.25E-5	0.0
7	0.00100625	1.25E-5	0.0
8	0.00103125	1.25E-5	0.0
9	0.0011	3.125E-5	0.0
10-11	0.001296875	3.125E-5	0.0
12-13	0.00231875	3.125E-5	0.0
14-15	0.0038125	3.125E-5	0.0
16-17	0.005346875	3.75E-5	0.0
18-19	0.005971875	4.0625E-5	0.0
20-21	0.0068468750000000005	4.375E-5	0.0
22-23	0.015821875	4.375E-5	0.0
24-25	0.037715625	4.375E-5	0.0
26-27	0.041990625000000004	4.375E-5	0.0
28-29	0.043503125000000004	4.375E-5	0.0
30-31	0.045225	4.375E-5	6.25E-6
32-33	0.047325	4.6875E-5	6.25E-6
34-35	0.050084375	5.0E-5	6.25E-6
36-37	0.053390625	5.0E-5	6.25E-6
38-39	0.05725	5.0E-5	6.25E-6
40-41	0.062228124999999995	5.3125000000000004E-5	9.375000000000001E-6
42-43	0.06880312499999999	5.625E-5	1.25E-5
44-45	0.07714375000000001	5.625E-5	1.25E-5
46-47	0.088159375	5.625E-5	1.25E-5
48-49	0.102128125	5.625E-5	1.5625E-5
50-51	0.119953125	5.625E-5	1.875E-5
52-53	0.142459375	5.625E-5	1.875E-5
54-55	0.171059375	5.625E-5	3.125E-5
56-57	0.20694375	5.625E-5	4.375E-5
58-59	0.253784375	5.625E-5	4.375E-5
60-61	0.31386875000000003	5.625E-5	4.375E-5
62-63	0.389859375	5.625E-5	4.375E-5
64-65	0.48625312500000006	5.625E-5	5.0E-5
66-67	0.6067374999999999	5.625E-5	5.0E-5
68-69	0.76234375	5.625E-5	5.0E-5
70-71	0.9611562499999999	7.500000000000001E-5	5.0E-5
72-73	1.210334375	8.125E-5	5.3125000000000004E-5
74-75	1.51914375	9.999999999999999E-5	6.25E-5
76-77	1.9062437500000002	1.1875E-4	6.25E-5
78-79	2.38729375	1.1875E-4	6.25E-5
80-81	2.986465625	1.21875E-4	6.562500000000001E-5
82-83	3.7197750000000003	1.25E-4	6.875E-5
84-85	4.611553125	1.25E-4	7.5E-5
86-87	5.6844375	1.25E-4	7.5E-5
88-89	6.952990625	1.25E-4	8.125E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAAAT	132815	0.0	72.492195	1
ATAAATC	139905	0.0	70.260475	2
TAAATCA	151790	0.0	65.351875	3
TCATAAA	171305	0.0	59.824753	7
AATCATA	173430	0.0	58.516617	5
ATCATAA	187190	0.0	55.143833	6
AAATCAT	182020	0.0	54.688675	4
CATAAAC	216465	0.0	47.468887	8
ATAAACA	234250	0.0	43.895317	9
ACAAACG	180710	0.0	29.478258	12-13
GTCCCCA	3180	0.0	28.680065	1
AAACGGG	190070	0.0	27.70549	14-15
TGATACG	2735	0.0	27.265223	3
CAAACGG	186955	0.0	27.11659	14-15
ACGGGCA	205660	0.0	25.612211	16-17
AACGGGC	202470	0.0	24.587103	16-17
GGGCAAC	211980	0.0	24.559986	18-19
GTCCCCC	2290	0.0	24.061827	1
GATACGG	3115	0.0	23.786648	4
ATACGGC	3150	0.0	23.371202	5
>>END_MODULE