##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2212_lane2_CTTGTA_L002_R1_004.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10634369
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.421412497535115	31.0	27.0	33.0	16.0	34.0
2	29.046268001420678	31.0	28.0	34.0	16.0	34.0
3	30.07399320072493	31.0	30.0	34.0	25.0	34.0
4	33.42079158622388	35.0	33.0	37.0	26.0	37.0
5	33.714253473807425	35.0	33.0	37.0	28.0	37.0
6	34.74610275419256	36.0	35.0	37.0	32.0	37.0
7	34.991822645988684	36.0	35.0	37.0	32.0	37.0
8	35.062032077314605	37.0	35.0	37.0	32.0	37.0
9	36.75025598603923	39.0	37.0	39.0	32.0	39.0
10-11	36.7592794175188	39.0	37.0	39.0	32.0	39.0
12-13	36.8620720232672	39.0	37.0	39.0	33.0	39.0
14-15	38.02392643136608	40.0	37.0	41.0	33.0	41.0
16-17	37.986448138107676	40.0	37.0	41.0	33.0	41.0
18-19	38.20302770197273	40.0	37.5	41.0	33.5	41.0
20-21	38.42464235536683	40.0	38.0	41.0	34.0	41.0
22-23	38.43138892396907	40.0	38.0	41.0	34.0	41.0
24-25	38.38196351847486	40.0	38.0	41.0	34.0	41.0
26-27	38.21527920462418	40.0	38.0	41.0	33.5	41.0
28-29	38.06872429384386	40.0	37.5	41.0	33.0	41.0
30-31	37.984327513931476	40.0	37.0	41.0	33.0	41.0
32-33	37.86560246310806	40.0	37.0	41.0	33.0	41.0
34-35	37.77603974434214	40.0	37.0	41.0	33.0	41.0
36-37	37.684632299292986	40.0	37.0	41.0	32.0	41.0
38-39	37.58314386119196	40.0	37.0	41.0	32.0	41.0
40-41	37.469343973300155	40.0	37.0	41.0	32.0	41.0
42-43	37.33554082992606	40.0	36.0	41.0	31.5	41.0
44-45	37.19087526490759	40.0	36.0	41.0	31.0	41.0
46-47	37.04545981994795	40.0	35.5	41.0	31.0	41.0
48-49	36.89699661540802	39.0	35.0	41.0	31.0	41.0
50-51	36.728155662080184	39.0	35.0	41.0	30.0	41.0
52-53	36.57204245028548	39.0	35.0	41.0	30.0	41.0
54-55	36.38421748389585	39.0	35.0	41.0	30.0	41.0
56-57	36.16610068730923	39.0	35.0	41.0	30.0	41.0
58-59	35.96171018703602	38.0	34.5	40.0	29.5	41.0
60-61	35.65710429081406	37.5	34.0	40.0	29.0	41.0
62-63	35.38321215861515	37.0	34.0	40.0	29.0	41.0
64-65	35.03643685864201	36.5	34.0	39.0	28.5	41.0
66-67	34.624053105548626	36.0	34.0	39.0	28.0	40.5
68-69	34.19772047593985	35.5	33.0	38.5	28.0	40.0
70-71	33.79762015028818	35.0	33.0	37.0	28.0	39.0
72-73	33.40204289506975	35.0	33.0	37.0	27.0	39.0
74-75	33.01484507449384	35.0	33.0	36.0	27.0	38.5
76-77	31.145940581900064	33.5	30.0	35.0	24.0	36.5
78-79	32.220662081596004	35.0	32.0	35.0	26.0	37.0
80-81	32.23220667817715	35.0	33.0	35.0	26.0	36.0
82-83	32.080249284184134	35.0	33.0	35.0	26.0	36.0
84-85	31.935547280708427	35.0	33.0	35.0	26.0	36.0
86-87	31.76450779543196	35.0	32.0	35.0	26.0	35.5
88-89	31.581040257301588	35.0	32.0	35.0	25.0	35.0
90-91	31.429377050956198	34.5	32.0	35.0	25.0	35.0
92-93	31.238104066165093	34.0	32.0	35.0	24.5	35.0
94-95	31.055558773632924	34.0	32.0	35.0	24.0	35.0
96-97	30.825652325963112	34.0	32.0	35.0	23.0	35.0
98-99	30.539484195066017	34.0	32.0	35.0	20.0	35.0
100-101	29.368865045025238	33.5	30.0	35.0	9.5	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2302	1	-4.953545887371664
2302	2	-5.746229945103508
2302	3	-2.7885227514057114
2302	4	-3.793919435968366
2302	5	-3.865795086773062
2302	6	-1.9625330183876173
2302	7	-0.849934984929277
2302	8	-0.47950117271225423
2302	9	-0.35539401782364166
2302	10-11	-0.2505947166316602
2302	12-13	-0.3960648444798238
2302	14-15	-0.52079228285168
2302	16-17	-0.5758352001599576
2302	18-19	-0.4318133996399425
2302	20-21	-0.6689684774753815
2302	22-23	-0.47959422803815244
2302	24-25	-0.4148799276688351
2302	26-27	-0.7009333690683874
2302	28-29	-0.628833727096783
2302	30-31	-0.462320004735858
2302	32-33	-0.4966081675008098
2302	34-35	-0.5282253916876911
2302	36-37	-0.43971208172347787
2302	38-39	-0.4805057033846367
2302	40-41	-0.4442740993931338
2302	42-43	-0.34978043551635096
2302	44-45	-0.4108683230334904
2302	46-47	-0.5526172400844303
2302	48-49	-0.5929424293920249
2302	50-51	-0.6401013556507209
2302	52-53	-0.6283174141968857
2302	54-55	-0.49609841114223485
2302	56-57	-0.47443081510784424
2302	58-59	-0.5528246504232897
2302	60-61	-0.67349929874905
2302	62-63	-0.6512790172649048
2302	64-65	-0.5914693388530168
2302	66-67	-0.560645898344859
2302	68-69	-0.5666084943749539
2302	70-71	-0.5975634020698024
2302	72-73	-0.6265127701584348
2302	74-75	-0.6743328556554076
2302	76-77	-0.6881827962256182
2302	78-79	-0.5076628567562835
2302	80-81	-0.4501255865783591
2302	82-83	-0.400643477679985
2302	84-85	-0.5248547563696491
2302	86-87	-0.4751139200846346
2302	88-89	-0.4705801236208558
2302	90-91	-0.5725731680462331
2302	92-93	-0.5456120821880468
2302	94-95	-0.4445012299349571
2302	96-97	-0.45294990016054015
2302	98-99	-0.451199861914521
2302	100-101	-0.217104851928692
2306	1	-3.0252108066315557
2306	2	-4.527452193901318
2306	3	-3.818822103669717
2306	4	-3.5809387227506484
2306	5	-4.028111619177398
2306	6	-2.3124722970450406
2306	7	-0.9406045122283757
2306	8	-0.47296187237795095
2306	9	-0.3559450998139013
2306	10-11	-0.26428421001779157
2306	12-13	-0.3098403112765311
2306	14-15	-0.30851418031783595
2306	16-17	-0.35059662640097145
2306	18-19	-0.5157268678389002
2306	20-21	-0.7604585143980032
2306	22-23	-0.6115738435741847
2306	24-25	-0.6225429180327708
2306	26-27	-0.6213222381627617
2306	28-29	-0.796313277970242
2306	30-31	-0.9526375482100704
2306	32-33	-0.9286180263892163
2306	34-35	-0.8970727749584739
2306	36-37	-0.8316443023693267
2306	38-39	-0.7823783135659355
2306	40-41	-0.8223153675772821
2306	42-43	-0.8986571582639158
2306	44-45	-0.9110135354216666
2306	46-47	-0.9387032815888432
2306	48-49	-0.9262127412303798
2306	50-51	-0.97599152992656
2306	52-53	-1.0896581886541554
2306	54-55	-0.9478517680487428
2306	56-57	-1.016890734009536
2306	58-59	-1.0480851626578271
2306	60-61	-1.0515645957024589
2306	62-63	-0.9948740732157759
2306	64-65	-1.0254498712580329
2306	66-67	-1.054547872496471
2306	68-69	-0.9363124698844061
2306	70-71	-0.9233294469632156
2306	72-73	-0.9955825545274095
2306	74-75	-0.9312592050811404
2306	76-77	-1.331940189243781
2306	78-79	-0.9416155470572996
2306	80-81	-0.8632807363089086
2306	82-83	-0.8808935694010813
2306	84-85	-0.9244147100681488
2306	86-87	-0.8894597936093476
2306	88-89	-0.9060071187842098
2306	90-91	-0.9666456516273243
2306	92-93	-0.9513261405554232
2306	94-95	-0.9139183003609901
2306	96-97	-0.9655828317380113
2306	98-99	-1.0109654968669375
2306	100-101	-0.9022652575369214
2303	1	3.6698442946795318
2303	2	3.4263227851446203
2303	3	2.42175404330861
2303	4	2.471694034301507
2303	5	2.44595153257508
2303	6	1.3059972818469916
2303	7	0.6123905764253053
2303	8	0.22418187242243448
2303	9	0.1395877720189418
2303	10-11	0.08861976771989077
2303	12-13	0.14678663642486356
2303	14-15	0.08907170113386087
2303	16-17	0.09928683193058419
2303	18-19	0.30751269457591235
2303	20-21	0.6000554817744259
2303	22-23	0.5542390595368829
2303	24-25	0.46603415315404817
2303	26-27	0.5477856309381011
2303	28-29	0.5783858276631335
2303	30-31	0.610977948852856
2303	32-33	0.6517524172499876
2303	34-35	0.6626241000040309
2303	36-37	0.6771296356493295
2303	38-39	0.6730870157893278
2303	40-41	0.6398121378501216
2303	42-43	0.7066148750901036
2303	44-45	0.7017876760824677
2303	46-47	0.6463371904965243
2303	48-49	0.7047860478064365
2303	50-51	0.7196143939716819
2303	52-53	0.6793807349797305
2303	54-55	0.6295409031403878
2303	56-57	0.678823834861042
2303	58-59	0.7089152592566421
2303	60-61	0.7532393641223365
2303	62-63	0.7193231556279258
2303	64-65	0.6676013474479703
2303	66-67	0.6153786966620913
2303	68-69	0.6461587287457888
2303	70-71	0.5672326676938155
2303	72-73	0.5125062629515753
2303	74-75	0.5358992302673613
2303	76-77	0.5584930240648163
2303	78-79	0.5046001873183599
2303	80-81	0.38114534998467775
2303	82-83	0.3890228267253377
2303	84-85	0.36937979776780594
2303	86-87	0.3590672814722389
2303	88-89	0.32726589335235445
2303	90-91	0.36922086218189776
2303	92-93	0.35641002799081534
2303	94-95	0.3330289487003135
2303	96-97	0.3278204935463904
2303	98-99	0.2800272420796013
2303	100-101	0.03144853663864566
2307	1	3.3805638004246568
2307	2	3.2568564254943837
2307	3	2.319354456701909
2307	4	1.3978502154787051
2307	5	2.135415435174785
2307	6	1.1358226986316922
2307	7	0.500902961530791
2307	8	0.2902606385242166
2307	9	0.2645410041851193
2307	10-11	0.12929555438764595
2307	12-13	0.16470336663400076
2307	14-15	0.1607412788662117
2307	16-17	0.20611118201683354
2307	18-19	0.2370585336329114
2307	20-21	0.5205124239870926
2307	22-23	0.42905153832650456
2307	24-25	0.46933950839392224
2307	26-27	0.5140787952525301
2307	28-29	0.5213437980807427
2307	30-31	0.5875401488109375
2307	32-33	0.6449979533679766
2307	34-35	0.6217363354629768
2307	36-37	0.6137396197697029
2307	38-39	0.6289006767590664
2307	40-41	0.6005319166110397
2307	42-43	0.6286690014584266
2307	44-45	0.6919770505492124
2307	46-47	0.6483608825526801
2307	48-49	0.6311272641477217
2307	50-51	0.6427944134201624
2307	52-53	0.6874507477694323
2307	54-55	0.6188080580269073
2307	56-57	0.6086606792739744
2307	58-59	0.6208521833917899
2307	60-61	0.6171766151987583
2307	62-63	0.5921976455666069
2307	64-65	0.5070331052715531
2307	66-67	0.5208825339900329
2307	68-69	0.547100750670694
2307	70-71	0.47653790763058623
2307	72-73	0.5044207346229896
2307	74-75	0.49087304630164397
2307	76-77	0.5056722495879562
2307	78-79	0.48549016347047314
2307	80-81	0.3921377360997127
2307	82-83	0.36960817563618775
2307	84-85	0.3411646240506485
2307	86-87	0.2920753453765208
2307	88-89	0.24411916077889373
2307	90-91	0.21512039384088055
2307	92-93	0.1795908348351496
2307	94-95	0.16294351115033834
2307	96-97	0.13164314410992262
2307	98-99	0.09763453945443956
2307	100-101	-0.08794596124906207
2304	1	3.3091384090653655
2304	2	3.24321634701424
2304	3	2.3191280260463074
2304	4	2.239694325421901
2304	5	2.419718190463051
2304	6	1.2660941973601112
2304	7	0.586642621529208
2304	8	0.2034439907438781
2304	9	0.07039578968755933
2304	10-11	0.04991786715469004
2304	12-13	0.2056122821634574
2304	14-15	0.1755718729967768
2304	16-17	0.20636845661918102
2304	18-19	0.2589570263515171
2304	20-21	0.5512041543372774
2304	22-23	0.45011858370754965
2304	24-25	0.4915520682368353
2304	26-27	0.4393362997111083
2304	28-29	0.47851302000140805
2304	30-31	0.5556176375617952
2304	32-33	0.5457553215764079
2304	34-35	0.5843074738232232
2304	36-37	0.6063937969022319
2304	38-39	0.5486287017153231
2304	40-41	0.5743172474740632
2304	42-43	0.5490830815754251
2304	44-45	0.5125564901533153
2304	46-47	0.5581112486332245
2304	48-49	0.5057566595918246
2304	50-51	0.5409279097732593
2304	52-53	0.5688134965264595
2304	54-55	0.558724836755033
2304	56-57	0.515728482917801
2304	58-59	0.5484479849135795
2304	60-61	0.5132344058564371
2304	62-63	0.47114367923065714
2304	64-65	0.44152021713411926
2304	66-67	0.46630986550306375
2304	68-69	0.46813169762778273
2304	70-71	0.44155011067108774
2304	72-73	0.4215365039575687
2304	74-75	0.39287485126627075
2304	76-77	0.43662868696186763
2304	78-79	0.37665971221910866
2304	80-81	0.33904554049720304
2304	82-83	0.316097580389723
2304	84-85	0.26808962938011405
2304	86-87	0.24730170597022294
2304	88-89	0.22384987431089343
2304	90-91	0.27701780890153316
2304	92-93	0.27449089275100036
2304	94-95	0.24757543178040464
2304	96-97	0.21547032767536933
2304	98-99	0.2249185041885724
2304	100-101	0.09866659948490764
2301	1	3.217014509708143
2301	2	3.1952109265996462
2301	3	2.2699626989397217
2301	4	1.5472251052695114
2301	5	2.1132659430504006
2301	6	1.124253336294096
2301	7	0.5038758689080112
2301	8	0.2606210595626166
2301	9	0.08213911942696228
2301	10-11	0.041018411071974015
2301	12-13	0.13551039496714168
2301	14-15	0.18965567622209534
2301	16-17	0.2559769942481296
2301	18-19	0.3021300541618004
2301	20-21	0.49222148788114595
2301	22-23	0.3818801535943024
2301	24-25	0.4035524922355833
2301	26-27	0.4032873704782318
2301	28-29	0.49535409325781643
2301	30-31	0.5235404589434793
2301	32-33	0.46689348612605386
2301	34-35	0.5230149192431384
2301	36-37	0.5163404177567585
2301	38-39	0.4996150880351138
2301	40-41	0.524056410062741
2301	42-43	0.4852879725013608
2301	44-45	0.5889164432328045
2301	46-47	0.5265022555509589
2301	48-49	0.5644086657422704
2301	50-51	0.5680219148905081
2301	52-53	0.571321904889686
2301	54-55	0.5879815345036832
2301	56-57	0.5289774613327296
2301	58-59	0.5617915460252334
2301	60-61	0.5821194752748724
2301	62-63	0.5382126596907639
2301	64-65	0.5314581902981459
2301	66-67	0.5338097176504846
2301	68-69	0.4954560425727408
2301	70-71	0.5027992525611467
2301	72-73	0.533316119006038
2301	74-75	0.48334327244511854
2301	76-77	0.5618855575803181
2301	78-79	0.5084114429092494
2301	80-81	0.4474366407971786
2301	82-83	0.4473379128417605
2301	84-85	0.5118448315636499
2301	86-87	0.5139719147103854
2301	88-89	0.4701218269675991
2301	90-91	0.5019783485725213
2301	92-93	0.4520632625804275
2301	94-95	0.42301274794783694
2301	96-97	0.47607964032520655
2301	98-99	0.44218502932662673
2301	100-101	0.3485631376393208
2305	1	3.5632518304570127
2305	2	3.2925580893768114
2305	3	2.3358241868559233
2305	4	2.721405444467983
2305	5	2.472644097140069
2305	6	1.2882181260365755
2305	7	0.5882384536315897
2305	8	0.21184548174961293
2305	9	0.13728423561897074
2305	10-11	0.05385021406010537
2305	12-13	0.17244381139133225
2305	14-15	0.15571461854933233
2305	16-17	0.12590021188178468
2305	18-19	0.16017168239996948
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2313	40-41	-0.2297230818595395
2313	42-43	-0.3018062798785053
2313	44-45	-0.2818511546439666
2313	46-47	-0.33294808026792566
2313	48-49	-0.2658413793545691
2313	50-51	-0.27598697972513264
2313	52-53	-0.2725325661558742
2313	54-55	-0.36827368416603434
2313	56-57	-0.3315263668470507
2313	58-59	-0.2704580728780144
2313	60-61	-0.3251393362033781
2313	62-63	-0.3647043216725976
2313	64-65	-0.3649616075364861
2313	66-67	-0.31877959116269494
2313	68-69	-0.27651427185392663
2313	70-71	-0.21531094440349818
2313	72-73	-0.24207419193772495
2313	74-75	-0.29490886446725995
2313	76-77	-0.2813339551436087
2313	78-79	-0.2194671394045784
2313	80-81	-0.2034596222124776
2313	82-83	-0.23249850286617146
2313	84-85	-0.20262824415019765
2313	86-87	-0.17524870645062052
2313	88-89	-0.16589120956237835
2313	90-91	-0.11676904697085888
2313	92-93	-0.11765513048733922
2313	94-95	-0.14168548253805469
2313	96-97	-0.05603618182315273
2313	98-99	-0.029946481347217002
2313	100-101	-0.012124206033540474
2316	1	-3.5888610277925324
2316	2	-2.8488999414300302
2316	3	-2.426513538339254
2316	4	-2.713583526390579
2316	5	-2.357962589203588
2316	6	-1.1937908786294926
2316	7	-0.5647823861098402
2316	8	-0.32463602754282306
2316	9	-0.2584101696278225
2316	10-11	-0.13361181986536508
2316	12-13	-0.1398878943884938
2316	14-15	-0.131695905617093
2316	16-17	-0.1032517238116526
2316	18-19	-0.15557013971952927
2316	20-21	-0.44697001006690584
2316	22-23	-0.3463370863488322
2316	24-25	-0.2641031043546249
2316	26-27	-0.2733173715194397
2316	28-29	-0.35182920334136014
2316	30-31	-0.3473425977522595
2316	32-33	-0.3329949627581641
2316	34-35	-0.44281445570945266
2316	36-37	-0.5015308027995431
2316	38-39	-0.4679090770438137
2316	40-41	-0.4665234075888094
2316	42-43	-0.5426847376780088
2316	44-45	-0.48538091719539267
2316	46-47	-0.38163051664798786
2316	48-49	-0.38799281479826675
2316	50-51	-0.38790853386016977
2316	52-53	-0.4358805661901499
2316	54-55	-0.46502985789730644
2316	56-57	-0.39653867115071506
2316	58-59	-0.44092473153523315
2316	60-61	-0.4898616882934874
2316	62-63	-0.47350660916758613
2316	64-65	-0.43706607493454186
2316	66-67	-0.4006448885243543
2316	68-69	-0.39236972815578497
2316	70-71	-0.32376717079549167
2316	72-73	-0.35874399088888964
2316	74-75	-0.31534470610511534
2316	76-77	-0.3230822787269574
2316	78-79	-0.4277642097611931
2316	80-81	-0.403113015171769
2316	82-83	-0.3786427948489788
2316	84-85	-0.3356214780440574
2316	86-87	-0.2927812621975683
2316	88-89	-0.2831297538084421
2316	90-91	-0.30842257736134115
2316	92-93	-0.2670735844128309
2316	94-95	-0.3448752069979477
2316	96-97	-0.3308735409000647
2316	98-99	-0.2252907014514669
2316	100-101	-0.23654144936941535
2216	1	-1.576929605804569
2216	2	-2.278922095190307
2216	3	-0.5845855339276298
2216	4	-1.4309703514267618
2216	5	-3.0968209550292265
2216	6	-1.307747111081774
2216	7	-0.6638010430369263
2216	8	-0.35169284642600473
2216	9	-0.23849948549373323
2216	10-11	-0.19883047224911365
2216	12-13	-0.20284706067587877
2216	14-15	-0.257506017106202
2216	16-17	-0.25022062977755155
2216	18-19	-0.26163610227161627
2216	20-21	-0.8182053464325278
2216	22-23	-0.5860627593943448
2216	24-25	-0.47655887514100215
2216	26-27	-0.4776561784795348
2216	28-29	-0.4587197741123319
2216	30-31	-0.5080079860794271
2216	32-33	-0.47130280115387535
2216	34-35	-0.4707039944201057
2216	36-37	-0.6323318520362378
2216	38-39	-0.5188144700968351
2216	40-41	-0.5464004933767939
2216	42-43	-0.5700397367273595
2216	44-45	-0.5360383488228777
2216	46-47	-0.5807236337739567
2216	48-49	-0.6025169483479971
2216	50-51	-0.6018767141086698
2216	52-53	-0.6183930885214295
2216	54-55	-0.6016634338124689
2216	56-57	-0.5960079879743319
2216	58-59	-0.5880713004839322
2216	60-61	-0.5823195363856257
2216	62-63	-0.5612547189663033
2216	64-65	-0.5538463268544191
2216	66-67	-0.5381455719340735
2216	68-69	-0.498084117305055
2216	70-71	-0.5014668218720999
2216	72-73	-0.4452804629495333
2216	74-75	-0.5322010452545669
2216	76-77	-0.49491405742597294
2216	78-79	-0.5205455479270356
2216	80-81	-0.4141453744252104
2216	82-83	-0.42204433210719117
2216	84-85	-0.3820427136264257
2216	86-87	-0.40931221469462287
2216	88-89	-0.35439481271222917
2216	90-91	-0.3624406554909356
2216	92-93	-0.34816014830733977
2216	94-95	-0.38154586768396115
2216	96-97	-0.40478528023338356
2216	98-99	-0.36822144877378093
2216	100-101	-0.27589270887871464
2215	1	-2.6025100639103798
2215	2	-2.8778563308627163
2215	3	-1.0530148336686338
2215	4	-0.31378366935042123
2215	5	-2.4396039013164987
2215	6	-1.2967817780701694
2215	7	-0.7952545167754153
2215	8	-0.4560713788496358
2215	9	-0.5043915528214242
2215	10-11	-0.4706830978253933
2215	12-13	-0.6015040124234119
2215	14-15	-0.5845198328016536
2215	16-17	-0.6441300750525372
2215	18-19	-0.6811938108503028
2215	20-21	-0.6405319054456768
2215	22-23	-0.7578761719040514
2215	24-25	-0.8585878813878409
2215	26-27	-0.8405207250776527
2215	28-29	-0.7808420266367904
2215	30-31	-0.8308127426724994
2215	32-33	-0.9362155961766376
2215	34-35	-0.9146314024793298
2215	36-37	-0.8413111104504338
2215	38-39	-0.958336200050077
2215	40-41	-0.8379543871820871
2215	42-43	-0.8848801767854155
2215	44-45	-1.0238558789248984
2215	46-47	-0.9861276850699312
2215	48-49	-0.9475661168025624
2215	50-51	-0.9312512821372536
2215	52-53	-0.9058931031792667
2215	54-55	-0.9230281036456276
2215	56-57	-0.965583398069974
2215	58-59	-1.045367148313673
2215	60-61	-1.2139520796504826
2215	62-63	-0.9821916726771178
2215	64-65	-0.8957469952765464
2215	66-67	-0.8857594322310192
2215	68-69	-0.8845137773161866
2215	70-71	-0.8359647361896378
2215	72-73	-0.8016911742910722
2215	74-75	-0.7367870910626095
2215	76-77	-0.6038367912115241
2215	78-79	-0.7959265407099849
2215	80-81	-0.897799741459739
2215	82-83	-0.8679430654038498
2215	84-85	-0.7567308679080753
2215	86-87	-0.85913953297106
2215	88-89	-0.756684236317426
2215	90-91	-0.9076425328468325
2215	92-93	-0.917648256966519
2215	94-95	-0.8998179695224877
2215	96-97	-0.8761373993190062
2215	98-99	-0.9646445672097954
2215	100-101	-0.7717062745681211
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1012.0
3	158.0
4	215.0
5	479.0
6	885.0
7	2242.0
8	4833.0
9	7505.0
10	10183.0
11	12354.0
12	14887.0
13	18647.0
14	20614.0
15	22662.0
16	24554.0
17	25994.0
18	27957.0
19	29734.0
20	31346.0
21	34690.0
22	39453.0
23	46926.0
24	58521.0
25	75579.0
26	97663.0
27	123858.0
28	156317.0
29	199893.0
30	259249.0
31	335494.0
32	438313.0
33	586022.0
34	815476.0
35	1168328.0
36	1574955.0
37	2133747.0
38	2119564.0
39	113991.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.942736803698997	25.483283698020447	15.262440251903522	44.31153924637703
2	17.07868173609726	29.04014806493273	23.602282664578215	30.278887534391796
3	11.98404907710086	18.501972237374876	30.633843907428826	38.88013477809544
4	16.975066844096332	21.089313567811693	14.26693234097603	47.66868724711594
5	19.829888127996437	24.869967885039124	16.78856785630191	38.511576130662526
6	16.13761944878911	31.308279786040906	19.55201103140205	33.00208973376794
7	12.640590146909517	32.70802433129789	23.753576728435885	30.897808793356713
8	11.55403766786727	35.53482110692228	20.48606739149262	32.425073833717825
9	11.594782915657714	40.31867805226619	21.839349377475994	26.2471896546001
10-11	11.946975469424068	37.05281868840074	23.21433793718306	27.785867904992127
12-13	11.115107064650473	40.05494825315917	20.23137432977923	28.59857035241113
14-15	11.58289222425891	36.972456945964545	20.124494457546092	31.320156372230457
16-17	11.422513299820562	38.907017765254004	20.04614008523696	29.62432884968847
18-19	14.367086566208112	32.082415985377224	20.973360055495537	32.577137392919134
20-21	17.114659083204657	32.890677387628735	21.094570820327938	28.90009270883867
22-23	10.314565913595814	36.37157503186132	20.508301903009006	32.80555715153386
24-25	10.298086327453937	35.86817421889348	20.352063201869335	33.48167625178325
26-27	10.3844304797629	36.34973589167386	23.797278023352117	29.468555605211122
28-29	10.506292005770934	35.907813634665665	19.927437818705506	33.6584565408579
30-31	10.325100130591933	32.714410053965864	23.694932994455474	33.265556820986724
32-33	10.373352562827368	36.36698913244273	21.72405603967555	31.53560226505435
34-35	10.501081354689745	35.80480460500284	21.301655287306033	32.39245875300138
36-37	10.439803006456001	35.65816554170286	21.566941181001116	32.33509027084003
38-39	10.387148993931689	36.08033594930763	21.826930061731602	31.705584995029078
40-41	10.457068381119763	35.77181185964843	21.453180488222305	32.31793927100951
42-43	10.397778425997027	35.450269139503995	21.786471877409078	32.3654805570899
44-45	10.443704762541515	35.96040303918424	21.84411086351609	31.751781334758157
46-47	10.497174887827015	35.688473486853674	21.469197501513136	32.345154123806175
48-49	10.459587091221513	35.4573301013238	21.78766859714536	32.295414210309325
50-51	10.353974045689831	35.82238613322681	21.999184519870088	31.824455301213273
52-53	10.477672598332264	35.520957973468185	21.750507822963204	32.250861605236345
54-55	10.321557395648016	35.35514895147986	22.00907265865986	32.314220994212256
56-57	10.404719453669383	35.76882553370716	22.016196263312914	31.81025874931055
58-59	10.505334974041581	35.524134298906844	21.767706876384675	32.2028238506669
60-61	10.396307246142099	35.38237985423157	21.995799140275395	32.22551375935094
62-63	10.459764245839265	35.810257527107105	22.12748786990923	31.602490357144404
64-65	10.458444773485843	35.71214371515154	21.67605863991639	32.15335287144623
66-67	10.390010464655235	35.50249833828873	21.88976242453889	32.21772877251715
68-69	10.463927171648022	35.90252597251164	22.008031543154615	31.62551531268572
70-71	10.553204554200853	35.732624185335126	21.654970692869785	32.05920056759423
72-73	10.526616461937673	35.433679221986566	21.921184744337484	32.11851957173828
74-75	10.5979757418339	35.78158256174483	22.02081451704021	31.599627179381063
76-77	10.749651381199078	35.6017367157276	21.661738411272523	31.9868734918008
78-79	10.785184255865438	35.410059403124784	21.880214443178524	31.924541897831254
80-81	10.940799585326642	35.758646488705196	21.958207854268494	31.342346071699673
82-83	11.172738714083083	35.59228311797815	21.549952683329646	31.68502548460913
84-85	11.313017718761213	35.40859675505861	21.7150836286711	31.56330189750908
86-87	11.592267486674574	35.763969634681665	21.73693145310267	30.906831425541093
88-89	11.942194293434822	35.592073909937874	21.30573496372321	31.159996832904095
90-91	12.247543731001189	35.417766496793654	21.39897827936196	30.935711492843197
92-93	12.682584432801495	35.71493030884773	21.331380743654137	30.271104514696646
94-95	13.217578174791452	35.47279562253799	20.865093876139326	30.444532326531238
96-97	13.672276154511554	35.29529286100619	20.861693805686805	30.17073717879545
98-99	14.27351730977174	35.52032565354841	20.722898556557517	29.483258480122327
100-101	15.098766085698173	35.139701283639866	20.18838165198142	29.57315097868054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	3.0
2	2.5
3	1.5
4	1.5
5	3.0
6	3.0
7	5.5
8	8.0
9	7.5
10	15.5
11	30.0
12	39.0
13	55.0
14	78.0
15	103.0
16	136.0
17	177.0
18	215.5
19	265.5
20	345.0
21	477.5
22	697.0
23	1070.5
24	1859.0
25	3551.5
26	7225.0
27	14667.0
28	28938.0
29	55287.5
30	98789.5
31	161038.0
32	242063.0
33	336096.0
34	431451.0
35	516793.5
36	582334.0
37	622498.0
38	636756.5
39	625517.5
40	590723.5
41	541479.0
42	483918.5
43	423573.0
44	368436.0
45	319667.5
46	276875.5
47	242355.0
48	213944.5
49	190357.0
50	176522.0
51	167565.0
52	158347.0
53	152875.5
54	150851.0
55	149929.0
56	148726.0
57	147426.0
58	145693.0
59	142346.0
60	136508.0
61	129276.5
62	121504.5
63	111254.0
64	100160.5
65	89051.5
66	77549.0
67	66128.0
68	55389.0
69	45398.0
70	36195.5
71	28080.5
72	21245.0
73	15966.0
74	11783.0
75	8544.0
76	6034.5
77	4125.0
78	2944.0
79	2108.0
80	1445.0
81	981.0
82	678.5
83	494.5
84	322.5
85	227.5
86	177.5
87	126.5
88	93.0
89	79.0
90	60.0
91	46.0
92	45.0
93	38.5
94	27.5
95	22.5
96	16.0
97	10.0
98	6.5
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013070827239491125
2	0.006168678179213078
3	0.0
4	0.002783427958913218
5	6.864535168941382E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.522778267333022E-5
12-13	0.0
14-15	0.0
16-17	1.4105209251249416E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.4105209251249416E-5
28-29	3.9024412261790047E-4
30-31	0.01532295898327395
32-33	9.87364647587459E-5
34-35	0.006737588285680138
36-37	0.0058254514207660086
38-39	2.4919203010540636E-4
40-41	6.958569897283045E-4
42-43	0.0010061715932557916
44-45	6.394361527233068E-4
46-47	1.1754341042707847E-4
48-49	0.006930359478780546
50-51	0.0013446966152857777
52-53	3.291215491958197E-5
54-55	0.0
56-57	2.350868208541569E-5
58-59	0.003230092918536116
60-61	0.014533067265203982
62-63	6.676465712258056E-4
64-65	0.0010437854845924566
66-67	4.7017364170831385E-6
68-69	4.6077016887414757E-4
70-71	1.0813993759291219E-4
72-73	0.0029244800514257123
74-75	0.0028257435866669662
76-77	0.0058254514207660086
78-79	0.012624162279868229
80-81	0.004297387085213989
82-83	0.006544817092579729
84-85	0.0020405536050140823
86-87	0.0
88-89	1.974729295174918E-4
90-91	0.0014199243979591079
92-93	0.018745823094910474
94-95	0.0016032921182253502
96-97	0.004800472881841884
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.0634369E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.11335814855588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5028724143012	78.84009138100078
2	7.230930892002091	12.598213450848695
3	1.5565490434830307	4.06788642902188
4	0.3833132750490406	1.3356682644977194
5	0.12614682832630608	0.5494536917646947
6	0.06453120077864316	0.33729177631117846
7	0.010246905842401439	0.06248496649945524
8	0.012557187015009859	0.08751189838215594
9	0.014356474924528772	0.11255766676661128
>10	0.09700389950570858	1.5453900125496567
>50	8.778978608624595E-4	0.05113553318882826
>100	5.2025527391926E-4	0.11128246980992569
>500	0.0	0.0
>1k	7.209868986891721E-5	0.12284788842856008
>5k	1.0839140780673796E-5	0.06668180175022843
>10k+	1.078780667378379E-5	0.11150276917963058
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	11865	0.11157220517738287	TruSeq Adapter, Index 12 (100% over 50bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.11225734220808E-4	0.0	0.0
2	6.488396255574731E-4	0.0	0.0
3	6.958569897283045E-4	1.8806945668332554E-5	0.0
4	6.958569897283045E-4	1.8806945668332554E-5	9.403472834166277E-6
5	7.428743538991359E-4	1.8806945668332554E-5	9.403472834166277E-6
6	9.027333920799626E-4	1.8806945668332554E-5	9.403472834166277E-6
7	0.0010813993759291218	1.8806945668332554E-5	9.403472834166277E-6
8	0.0011378202129341194	1.8806945668332554E-5	9.403472834166277E-6
9	0.0012036445227732835	3.761389133666511E-5	9.403472834166277E-6
10-11	0.0014340296072103574	3.761389133666511E-5	9.403472834166277E-6
12-13	0.0024354994640490655	3.761389133666511E-5	9.403472834166277E-6
14-15	0.003855423862008174	3.761389133666511E-5	9.403472834166277E-6
16-17	0.00530355867846978	8.463125550749649E-5	9.403472834166277E-6
18-19	0.005928889621941838	9.403472834166278E-5	9.403472834166277E-6
20-21	0.0065918344567505605	1.0343820117582906E-4	9.403472834166277E-6
22-23	0.016004710763751005	1.0343820117582906E-4	9.403472834166277E-6
24-25	0.03851662472874507	1.0343820117582906E-4	9.403472834166277E-6
26-27	0.042893941333049475	1.0343820117582906E-4	9.403472834166277E-6
28-29	0.044591268179616485	1.0343820117582906E-4	9.403472834166277E-6
30-31	0.046203963770676	1.0343820117582906E-4	1.8806945668332554E-5
32-33	0.04810346528317759	1.0343820117582906E-4	1.8806945668332554E-5
34-35	0.05095271755192997	1.0343820117582906E-4	1.8806945668332554E-5
36-37	0.05433796777222984	1.0343820117582906E-4	1.8806945668332554E-5
38-39	0.058038234332474264	1.0343820117582906E-4	1.8806945668332554E-5
40-41	0.06319603918201447	1.0343820117582906E-4	1.8806945668332554E-5
42-43	0.06974555801101129	1.0343820117582906E-4	1.8806945668332554E-5
44-45	0.0783544373906905	1.1284167400999533E-4	1.8806945668332554E-5
46-47	0.08952106138126295	1.1284167400999533E-4	1.8806945668332554E-5
48-49	0.10354634111342197	1.1754341042707847E-4	1.8806945668332554E-5
50-51	0.12143174644400621	1.222451468441616E-4	1.8806945668332554E-5
52-53	0.14468183302648235	1.222451468441616E-4	1.8806945668332554E-5
54-55	0.1734987755267849	1.222451468441616E-4	3.291215491958197E-5
56-57	0.21062838801249045	1.222451468441616E-4	7.052604625624708E-5
58-59	0.25807831193369346	1.222451468441616E-4	7.522778267333022E-5
60-61	0.3177997679034835	1.222451468441616E-4	7.522778267333022E-5
62-63	0.3947765965239687	1.222451468441616E-4	7.522778267333022E-5
64-65	0.49239404801544884	1.222451468441616E-4	7.522778267333022E-5
66-67	0.6162895043420066	1.222451468441616E-4	7.522778267333022E-5
68-69	0.7739951472438091	1.222451468441616E-4	7.522778267333022E-5
70-71	0.9723802136262152	1.36350356095411E-4	7.522778267333022E-5
72-73	1.218760605354206	1.4105209251249416E-4	7.522778267333022E-5
74-75	1.5295547859962353	1.457538289295773E-4	7.522778267333022E-5
76-77	1.9163290271383286	1.5045556534666043E-4	7.522778267333022E-5
78-79	2.4006266850435605	1.598590381808267E-4	7.522778267333022E-5
80-81	2.998607627777445	1.7866598384915926E-4	7.992951909041335E-5
82-83	3.730056762183069	1.8806945668332553E-4	8.463125550749649E-5
84-85	4.621924441403152	1.8806945668332553E-4	8.463125550749649E-5
86-87	5.692429894053893	1.8806945668332553E-4	8.463125550749649E-5
88-89	6.9676771607229355	1.8806945668332553E-4	8.463125550749649E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAAAT	86215	0.0	72.22506	1
ATAAATC	90850	0.0	69.97188	2
TAAATCA	98870	0.0	65.39004	3
TCATAAA	110805	0.0	59.8196	7
AATCATA	113270	0.0	58.20779	5
ATCATAA	122425	0.0	54.66166	6
AAATCAT	118990	0.0	54.488914	4
CATAAAC	139775	0.0	47.61497	8
ATAAACA	150295	0.0	44.25685	9
GTCCCCA	2000	0.0	30.163021	1
ACAAACG	117215	0.0	29.38249	12-13
TGATACG	1735	0.0	28.196287	3
AAACGGG	122915	0.0	27.562037	14-15
CAAACGG	121265	0.0	26.916788	14-15
ACGGGCA	133300	0.0	25.445045	16-17
AACGGGC	130980	0.0	24.40542	16-17
GGGCAAC	138135	0.0	24.305029	18-19
GTCCCCC	1565	0.0	23.371012	1
CGGGCAA	137455	0.0	23.136456	18-19
CCCCGCA	3885	0.0	22.986242	1
>>END_MODULE