##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2212_lane2_GCCAAT_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.4965269375 31.0 30.0 34.0 16.0 34.0 2 30.1453589375 31.0 30.0 34.0 25.0 34.0 3 31.0924225 31.0 31.0 34.0 27.0 34.0 4 33.9772890625 35.0 35.0 37.0 28.0 37.0 5 34.4154410625 35.0 35.0 37.0 30.0 37.0 6 35.1760423125 37.0 35.0 37.0 32.0 37.0 7 35.3592600625 37.0 35.0 37.0 33.0 37.0 8 35.4090050625 37.0 35.0 37.0 33.0 37.0 9 37.0843534375 39.0 37.0 39.0 33.0 39.0 10-11 37.10050521875 39.0 37.0 39.0 33.0 39.0 12-13 37.15461559375 39.0 37.0 39.0 33.5 39.0 14-15 38.3826855 40.0 38.0 41.0 33.5 41.0 16-17 38.316089125 40.0 38.0 41.0 33.0 41.0 18-19 38.32239790625 40.0 38.0 41.0 33.0 41.0 20-21 38.3088736875 40.0 38.0 41.0 33.5 41.0 22-23 38.32966896875 40.0 38.0 41.0 34.0 41.0 24-25 38.275583375 40.0 38.0 41.0 34.0 41.0 26-27 38.128494875 40.0 38.0 41.0 33.5 41.0 28-29 37.861497281249996 40.0 37.0 41.0 32.5 41.0 30-31 37.961290000000005 40.0 37.0 41.0 33.0 41.0 32-33 37.7104619375 40.0 37.0 41.0 32.5 41.0 34-35 37.58296025 40.0 37.0 41.0 32.0 41.0 36-37 37.48598609375 40.0 37.0 41.0 31.0 41.0 38-39 37.423097125 40.0 37.0 41.0 31.0 41.0 40-41 37.3528661875 40.0 37.0 41.0 31.0 41.0 42-43 37.27486225 40.0 37.0 41.0 31.0 41.0 44-45 37.19948328125 40.0 37.0 41.0 31.0 41.0 46-47 37.07317584374999 40.0 36.0 41.0 31.0 41.0 48-49 36.98613565625 40.0 36.0 41.0 30.0 41.0 50-51 36.891258750000006 40.0 36.0 41.0 30.0 41.0 52-53 36.739381406250004 39.5 36.0 41.0 30.0 41.0 54-55 36.6127721875 39.0 36.0 41.0 30.0 41.0 56-57 36.4695225 39.0 35.0 41.0 29.5 41.0 58-59 36.271276875 39.0 35.0 40.5 29.0 41.0 60-61 36.06329275 39.0 35.0 40.0 28.5 41.0 62-63 35.7711158125 38.0 35.0 40.0 28.0 41.0 64-65 35.438673875 37.5 34.0 40.0 28.0 41.0 66-67 34.98129828125 37.0 34.0 39.0 28.0 41.0 68-69 34.53509296875 36.5 34.0 39.0 28.0 40.0 70-71 34.1034310625 36.0 34.0 38.5 28.0 40.0 72-73 33.666348875 35.5 33.0 37.0 27.0 39.0 74-75 33.1899690625 35.0 33.0 37.0 26.0 39.0 76-77 30.436853093750003 32.5 29.5 34.5 23.0 36.5 78-79 32.3232533125 34.5 32.0 36.0 26.0 37.0 80-81 32.4106621875 35.0 33.0 35.5 26.0 37.0 82-83 32.2367066875 35.0 33.0 35.0 26.0 36.0 84-85 32.03837315625 35.0 33.0 35.0 26.0 36.0 86-87 31.89724409375 35.0 33.0 35.0 26.0 36.0 88-89 31.73302990625 35.0 32.5 35.0 26.0 35.5 90-91 31.611485593749997 35.0 32.0 35.0 25.0 35.0 92-93 31.42441653125 35.0 32.0 35.0 25.0 35.0 94-95 31.270258562499997 34.0 32.0 35.0 25.0 35.0 96-97 31.11280559375 34.0 32.0 35.0 24.0 35.0 98-99 30.911710375 34.0 32.0 35.0 23.5 35.0 100-101 30.0345810625 34.0 31.0 35.0 17.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.9858053286299864 1101 2 1.7465968557787157 1101 3 1.0967016460296222 1101 4 1.2242997421363384 1101 5 1.4057532580734673 1101 6 0.68377042914301 1101 7 0.3531445893328282 1101 8 0.24823953143037158 1101 9 0.20917974197652 1101 10-11 0.18047471962182726 1101 12-13 0.21905382972084908 1101 14-15 0.22127924230932905 1101 16-17 0.20431747041453008 1101 18-19 0.30486417516839026 1101 20-21 0.46884696070887344 1101 22-23 0.4639188426707719 1101 24-25 0.4976134770119174 1101 26-27 0.5049281304568751 1101 28-29 0.53506578567238 1101 30-31 0.523074766787559 1101 32-33 0.6291188430784729 1101 34-35 0.6871437502371052 1101 36-37 0.6396955309063088 1101 38-39 0.6592328503304969 1101 40-41 0.691659904598346 1101 42-43 0.7507070716314601 1101 44-45 0.6761914129111517 1101 46-47 0.6773396978248982 1101 48-49 0.7008303727414003 1101 50-51 0.7414485161962503 1101 52-53 0.726348094922642 1101 54-55 0.6786310753707738 1101 56-57 0.6970559912108527 1101 58-59 0.705690044880086 1101 60-61 0.729920911788561 1101 62-63 0.7902480431673595 1101 64-65 0.7450308441254307 1101 66-67 0.7132935319432718 1101 68-69 0.7656841954553215 1101 70-71 0.7233101297190032 1101 72-73 0.7049038116884034 1101 74-75 0.6970650595739443 1101 76-77 0.6563549424870132 1101 78-79 0.6397795247137452 1101 80-81 0.6222880889970241 1101 82-83 0.6289191123398297 1101 84-85 0.6057654713697218 1101 86-87 0.6719174527972562 1101 88-89 0.6306136401064926 1101 90-91 0.5927581866627811 1101 92-93 0.6423636094034393 1101 94-95 0.6401957945624943 1101 96-97 0.6261114110041497 1101 98-99 0.6716917245270011 1101 100-101 0.6338580815561095 1102 1 1.823436640467257 1102 2 1.7105041392459448 1102 3 1.1226358526862512 1102 4 0.42027506491558597 1102 5 1.1735854088962085 1102 6 0.5674854357803838 1102 7 0.3373244414948289 1102 8 0.21276844145282325 1102 9 0.22731138331821654 1102 10-11 0.18991281698637152 1102 12-13 0.22431808729363212 1102 14-15 0.22948740451671767 1102 16-17 0.266701489786378 1102 18-19 0.3027683517641435 1102 20-21 0.40615082592888996 1102 22-23 0.37031897901346866 1102 24-25 0.41493269345441774 1102 26-27 0.3519122155805263 1102 28-29 0.3624821954547315 1102 30-31 0.3288318353790416 1102 32-33 0.427571463406224 1102 34-35 0.4478627818197012 1102 36-37 0.524065827758676 1102 38-39 0.5784912937092059 1102 40-41 0.6019262201914088 1102 42-43 0.4074060303295042 1102 44-45 0.5619708324569217 1102 46-47 0.6171711044717441 1102 48-49 0.5835040335253936 1102 50-51 0.5512502668104133 1102 52-53 0.5733240292802648 1102 54-55 0.6039765036901343 1102 56-57 0.5627670871445432 1102 58-59 0.485854061858646 1102 60-61 0.5660029957383372 1102 62-63 0.5221711698273523 1102 64-65 0.572451222550626 1102 66-67 0.6035493478822147 1102 68-69 0.616893356484475 1102 70-71 0.5654426830115682 1102 72-73 0.5454712746403345 1102 74-75 0.5047599537010967 1102 76-77 0.2748726257220113 1102 78-79 0.5198756654327994 1102 80-81 0.508180432509171 1102 82-83 0.5059601920618917 1102 84-85 0.4980546130957606 1102 86-87 0.5177013484117232 1102 88-89 0.50392505976723 1102 90-91 0.5036692310036877 1102 92-93 0.5412000050143249 1102 94-95 0.558899308556672 1102 96-97 0.5477285916189558 1102 98-99 0.5058584070099172 1102 100-101 0.5316849496797964 1103 1 1.8358549238474318 1103 2 1.6831279464153468 1103 3 1.0126700690952717 1103 4 0.6310608788597989 1103 5 1.174706774301086 1103 6 0.5451276201767499 1103 7 0.3304870874725623 1103 8 0.24382076837635935 1103 9 0.23022120561846293 1103 10-11 0.21014793822338618 1103 12-13 0.19906223207046736 1103 14-15 0.27113377942605865 1103 16-17 0.23899337952265398 1103 18-19 0.2574455165737106 1103 20-21 0.36263794538457006 1103 22-23 0.18027248142539065 1103 24-25 0.33477125231073046 1103 26-27 0.35388162812087387 1103 28-29 0.3497341355349519 1103 30-31 0.4155925294442042 1103 32-33 0.4879837686100643 1103 34-35 0.40435230646493636 1103 36-37 0.44196188455730834 1103 38-39 0.5003894431060729 1103 40-41 0.5067889019272442 1103 42-43 0.5577094118035504 1103 44-45 0.5744962428763358 1103 46-47 0.5713005788242 1103 48-49 0.43231023080485187 1103 50-51 0.5728842908200775 1103 52-53 0.4574526022000285 1103 54-55 0.5250766303894494 1103 56-57 0.5760047930767129 1103 58-59 0.6001428742911443 1103 60-61 0.6157686911012021 1103 62-63 0.6215790465220934 1103 64-65 0.6483416648500082 1103 66-67 0.6111781608520701 1103 68-69 0.5707228587340154 1103 70-71 0.5581583369167475 1103 72-73 0.5291924501409682 1103 74-75 0.5051068491046777 1103 76-77 0.33110976568245576 1103 78-79 0.5527030447905901 1103 80-81 0.5463177926187868 1103 82-83 0.5395989957107616 1103 84-85 0.5530841866772533 1103 86-87 0.5564081565645189 1103 88-89 0.5469179752897197 1103 90-91 0.565565484555183 1103 92-93 0.5832135366695468 1103 94-95 0.6128933708885143 1103 96-97 0.5214356152343669 1103 98-99 0.6602404362863155 1103 100-101 0.6269626870443155 1202 1 1.4735153408186328 1202 2 1.4369367819047056 1202 3 0.696865500067954 1202 4 -0.040550306005805226 1202 5 0.9291542533131221 1202 6 0.4162848395343701 1202 7 0.21610384027245289 1202 8 0.12825858537590307 1202 9 0.13257297099147536 1202 10-11 0.16126832279664427 1202 12-13 0.13146619064643517 1202 14-15 0.13529212466174556 1202 16-17 0.16578671732485617 1202 18-19 0.22641855855777493 1202 20-21 0.262166818528911 1202 22-23 0.18505317135686994 1202 24-25 0.2789571541461129 1202 26-27 0.23199261395767223 1202 28-29 0.25643743987512835 1202 30-31 0.2707494812513076 1202 32-33 0.2299878379008362 1202 34-35 0.29785138706689196 1202 36-37 0.27936027353559467 1202 38-39 0.26402963083673825 1202 40-41 0.15972134689688033 1202 42-43 0.17803785089609647 1202 44-45 0.2633387694721421 1202 46-47 0.29187522534598287 1202 48-49 0.3501548867173838 1202 50-51 0.3993790588158177 1202 52-53 0.41932313990498216 1202 54-55 0.47543172255063126 1202 56-57 0.410269426075196 1202 58-59 0.43814813870400826 1202 60-61 0.26075072494327145 1202 62-63 0.37310688082877874 1202 64-65 0.43848577151901225 1202 66-67 0.3771777553541753 1202 68-69 0.3656797339100919 1202 70-71 0.37034945931078056 1202 72-73 0.3972325449125904 1202 74-75 0.4090122617085825 1202 76-77 0.2353997124969105 1202 78-79 0.33580529107288015 1202 80-81 0.39367519513260874 1202 82-83 0.3174919677720709 1202 84-85 0.38280118098929705 1202 86-87 0.34955284727422864 1202 88-89 0.38083123662487495 1202 90-91 0.3690697903082629 1202 92-93 0.3581696006404407 1202 94-95 0.3020242352673499 1202 96-97 0.34844708478792086 1202 98-99 0.3536538895357779 1202 100-101 0.3848020044004521 1203 1 0.6945999582053268 1203 2 0.9873350072571867 1203 3 0.4003534246340408 1203 4 1.0800184329493376 1203 5 1.1807787945912978 1203 6 0.4633053466820982 1203 7 0.3172870658673119 1203 8 0.20162560411635866 1203 9 0.15828926751607497 1203 10-11 0.16636633106226384 1203 12-13 0.09196382576367768 1203 14-15 0.16747250062513075 1203 16-17 0.13294117267473382 1203 18-19 0.18594262679586393 1203 20-21 0.19932376588151612 1203 22-23 0.15088372623073099 1203 24-25 0.17876757068951576 1203 26-27 0.12435595955298595 1203 28-29 0.17445022494804618 1203 30-31 0.21824252397738064 1203 32-33 0.22409961230223985 1203 34-35 0.29465528844165334 1203 36-37 0.3418699341987832 1203 38-39 0.31870977246312293 1203 40-41 0.29897734955684285 1203 42-43 0.32052837273281654 1203 44-45 0.3186600766995724 1203 46-47 0.40855090716890174 1203 48-49 0.39400478923055715 1203 50-51 0.28661755231775743 1203 52-53 0.2549705273825893 1203 54-55 0.38906197060819636 1203 56-57 0.3054559006799167 1203 58-59 0.3258775233068292 1203 60-61 0.34482030285687415 1203 62-63 0.3008698086939461 1203 64-65 0.33007907734162245 1203 66-67 0.42938732365717414 1203 68-69 0.3327040659371434 1203 70-71 0.32670038530681467 1203 72-73 0.32037474363432494 1203 74-75 0.28730343917844436 1203 76-77 0.07703850330544526 1203 78-79 0.264929901390893 1203 80-81 0.3288193550647449 1203 82-83 0.3371068775132784 1203 84-85 0.33843628737264453 1203 86-87 0.29928410936201644 1203 88-89 0.28008259502695765 1203 90-91 0.2714516227485184 1203 92-93 0.2477521338372668 1203 94-95 0.26518073009340526 1203 96-97 0.23369141560555562 1203 98-99 0.3293612516682174 1203 100-101 0.32008136825471567 1116 1 1.529364803628976 1116 2 1.5301078063711167 1116 3 0.9285845835261526 1116 4 1.3647344471556053 1116 5 1.3085406284753347 1116 6 0.5074117105070002 1116 7 0.2457342209436888 1116 8 0.146612139241725 1116 9 0.1288521253828847 1116 10-11 0.04963491678940102 1116 12-13 0.05573874931607037 1116 14-15 -0.01521034666765786 1116 16-17 0.041794821637459734 1116 18-19 0.03144113290423434 1116 20-21 0.03509365392129382 1116 22-23 0.18900498714421587 1116 24-25 0.021719598484637004 1116 26-27 0.11813323026052558 1116 28-29 0.14583805580863896 1116 30-31 0.07096964673765882 1116 32-33 0.04661168865698073 1116 34-35 0.1776069197857737 1116 36-37 0.13477803274466993 1116 38-39 0.025929535432737794 1116 40-41 0.1075058910801232 1116 42-43 0.18075151490609898 1116 44-45 0.17561820332515765 1116 46-47 0.08342999431261688 1116 48-49 0.059144364604406974 1116 50-51 0.0830201571382716 1116 52-53 0.1394100943780998 1116 54-55 0.18181965314504822 1116 56-57 0.21324507703318574 1116 58-59 0.16644925514645337 1116 60-61 0.19828544525207548 1116 62-63 0.19730425901480686 1116 64-65 0.2055681206501987 1116 66-67 0.2185005093895782 1116 68-69 0.23113520770552043 1116 70-71 0.1364690126806707 1116 72-73 0.24716727786248072 1116 74-75 0.29763690568699275 1116 76-77 0.3496715142145135 1116 78-79 0.3265284927892296 1116 80-81 0.35269384989545216 1116 82-83 0.2794949366549204 1116 84-85 0.292300000703122 1116 86-87 0.2627289506803372 1116 88-89 0.24848331291036274 1116 90-91 0.27251856414651243 1116 92-93 0.22368874290216922 1116 94-95 0.2830932740343819 1116 96-97 0.3103027839753665 1116 98-99 0.32066680644352985 1116 100-101 0.37402166433582806 1201 1 0.7432143628392502 1201 2 1.2026115146262875 1201 3 0.7821297128264177 1201 4 1.138472181238832 1201 5 1.2428298314592894 1201 6 0.5255678113862032 1201 7 0.1810339157223737 1201 8 0.07802022261019914 1201 9 0.06717936894428078 1201 10-11 0.0768552298819074 1201 12-13 0.08257021195056069 1201 14-15 0.05741440170660894 1201 16-17 0.10631024176323223 1201 18-19 0.0429079675975359 1201 20-21 0.07499071138107638 1201 22-23 0.07719868993666523 1201 24-25 0.04853032781937827 1201 26-27 0.08859850728430274 1201 28-29 0.14179726467315845 1201 30-31 0.10031085280548524 1201 32-33 0.17759955164666508 1201 34-35 0.1756178360284082 1201 36-37 0.16584402756309657 1201 38-39 0.19568111804994714 1201 40-41 0.2080725371671832 1201 42-43 0.13823158486131604 1201 44-45 0.17206767391017763 1201 46-47 0.1979811128907656 1201 48-49 0.09695091805107126 1201 50-51 0.15926207784963253 1201 52-53 0.16204810748455856 1201 54-55 0.12063997712633068 1201 56-57 0.1603596714312019 1201 58-59 0.19639544251224095 1201 60-61 0.22828013714548234 1201 62-63 0.23115033471525948 1201 64-65 0.2178825741167003 1201 66-67 0.25219886677167125 1201 68-69 0.3233268428060896 1201 70-71 0.3055213890545545 1201 72-73 0.3154309382900351 1201 74-75 0.2480096052639027 1201 76-77 0.3826337258292085 1201 78-79 0.26195923374121577 1201 80-81 0.24635482327823865 1201 82-83 0.2950193204107592 1201 84-85 0.2902615359198961 1201 86-87 0.2979811654727662 1201 88-89 0.2545531878073568 1201 90-91 0.25356422655764277 1201 92-93 0.3189463214903192 1201 94-95 0.3340248014308145 1201 96-97 0.2831578974658058 1201 98-99 0.2794001374547541 1201 100-101 0.2493213158776193 1113 1 -0.23630721980421399 1113 2 0.5114034712852025 1113 3 0.14370787669946594 1113 4 0.10768732937080472 1113 5 0.8189073053139211 1113 6 0.28917718111480895 1113 7 0.12044807051182715 1113 8 0.06011675168837627 1113 9 0.1083642863933818 1113 10-11 0.0030752479395630417 1113 12-13 -0.03694270268490385 1113 14-15 -0.059191772494394 1113 16-17 -0.025638014551091715 1113 18-19 -0.035423191727275594 1113 20-21 -0.05875840433167667 1113 22-23 0.02221522512621732 1113 24-25 0.05381320437521708 1113 26-27 0.07559845872617643 1113 28-29 0.061237049590012305 1113 30-31 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0.506389035132365 1104 42-43 0.5540519678010938 1104 44-45 0.4594976271151978 1104 46-47 0.5442629207860605 1104 48-49 0.57415363356575 1104 50-51 0.48213245520982895 1104 52-53 0.6017296712969724 1104 54-55 0.461900642840412 1104 56-57 0.546676093645452 1104 58-59 0.5488106793233953 1104 60-61 0.6407397972919071 1104 62-63 0.5631338657722935 1104 64-65 0.5454989852343743 1104 66-67 0.6214229397734599 1104 68-69 0.5181528788423506 1104 70-71 0.5006192650597612 1104 72-73 0.4539529545025047 1104 74-75 0.5568161841599917 1104 76-77 0.2587574365046521 1104 78-79 0.5512865914800855 1104 80-81 0.5061463104689992 1104 82-83 0.46618402345690413 1104 84-85 0.5457846663285295 1104 86-87 0.5568383702036108 1104 88-89 0.5926327105388758 1104 90-91 0.5313575672466051 1104 92-93 0.5566127303151163 1104 94-95 0.529093334380832 1104 96-97 0.6185608765541133 1104 98-99 0.6223044968326548 1104 100-101 0.49371805746033104 1107 1 1.5952431037186514 1107 2 1.600260926912057 1107 3 1.0682227372540822 1107 4 0.3771315094477643 1107 5 1.114059392285391 1107 6 0.5452475350171113 1107 7 0.28973688323267766 1107 8 0.18642508522589196 1107 9 0.26442657595254104 1107 10-11 0.14655045163816283 1107 12-13 0.20343863347260793 1107 14-15 0.2672530921461558 1107 16-17 0.3038125806104759 1107 18-19 0.293463474128842 1107 20-21 0.27058544400363616 1107 22-23 0.31242729750938025 1107 24-25 0.34619925687189834 1107 26-27 0.41489648221784137 1107 28-29 0.449056515480855 1107 30-31 0.36962059529924574 1107 32-33 0.46374616446730954 1107 34-35 0.523915543230629 1107 36-37 0.43945070879537695 1107 38-39 0.5352873485285556 1107 40-41 0.4851077000636508 1107 42-43 0.5530962897103464 1107 44-45 0.5367137876835812 1107 46-47 0.4741733773132992 1107 48-49 0.45272036776167823 1107 50-51 0.49591752198153216 1107 52-53 0.5418972698192519 1107 54-55 0.5556060577789736 1107 56-57 0.584494454136042 1107 58-59 0.6398399892888165 1107 60-61 0.6037281504138363 1107 62-63 0.5676565856292441 1107 64-65 0.5112103449899763 1107 66-67 0.5531102701860604 1107 68-69 0.5886082749113726 1107 70-71 0.5874472560021999 1107 72-73 0.4984203590804057 1107 74-75 0.500656028075035 1107 76-77 0.39227471575776107 1107 78-79 0.5351037085336543 1107 80-81 0.5340726580971378 1107 82-83 0.48614678904439756 1107 84-85 0.49747227018681883 1107 86-87 0.4861652592811545 1107 88-89 0.5059716932122491 1107 90-91 0.5031259881130161 1107 92-93 0.4716883378236574 1107 94-95 0.4560058091527708 1107 96-97 0.5073953358565717 1107 98-99 0.5381884304526139 1107 100-101 0.5199941957139309 1106 1 1.225938199861634 1106 2 1.2575130558302021 1106 3 0.19612477014391416 1106 4 -1.0926906444532491 1106 5 0.5141158047142866 1106 6 0.2673512531697071 1106 7 0.14574522537529333 1106 8 0.16648216759229229 1106 9 0.1722587434809668 1106 10-11 0.22576460892385342 1106 12-13 0.07858115765534279 1106 14-15 0.0740904920794705 1106 16-17 0.20068718584698075 1106 18-19 0.27640850997913446 1106 20-21 0.32687327307955627 1106 22-23 0.27961002662401313 1106 24-25 0.1591908896973493 1106 26-27 0.2543902220296701 1106 28-29 0.24142428719162723 1106 30-31 0.20507701499522568 1106 32-33 0.24105600413827233 1106 34-35 0.2248113021981979 1106 36-37 0.29588529705832656 1106 38-39 0.3099825098029356 1106 40-41 0.4110961916715965 1106 42-43 0.3481256915666009 1106 44-45 0.36935773087688517 1106 46-47 0.415715943359956 1106 48-49 0.43072581856159786 1106 50-51 0.48234401394254434 1106 52-53 0.4812481712129397 1106 54-55 0.4591138566347439 1106 56-57 0.4552866866318013 1106 58-59 0.4484254994886214 1106 60-61 0.4456877892700888 1106 62-63 0.40717686806458886 1106 64-65 0.3336376547169806 1106 66-67 0.35361031000420695 1106 68-69 0.42872291800217965 1106 70-71 0.3910127086151718 1106 72-73 0.3475773566364424 1106 74-75 0.4013344627366564 1106 76-77 0.41517104109737346 1106 78-79 0.37647801221106647 1106 80-81 0.35076074791023615 1106 82-83 0.3718703472350171 1106 84-85 0.3928674708900104 1106 86-87 0.37320605670292295 1106 88-89 0.33537006994923857 1106 90-91 0.3611924378284108 1106 92-93 0.4219283842751338 1106 94-95 0.4380070613288183 1106 96-97 0.4274343103585245 1106 98-99 0.39240567228091194 1106 100-101 0.30389555975778393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 444.0 3 87.0 4 119.0 5 282.0 6 685.0 7 1698.0 8 3805.0 9 6551.0 10 9168.0 11 13070.0 12 19385.0 13 32757.0 14 37258.0 15 42093.0 16 46331.0 17 47143.0 18 47883.0 19 49098.0 20 51644.0 21 54348.0 22 58426.0 23 65514.0 24 80882.0 25 103767.0 26 135128.0 27 174550.0 28 223499.0 29 287596.0 30 371166.0 31 474728.0 32 610956.0 33 798932.0 34 1076334.0 35 1514903.0 36 2225199.0 37 3610479.0 38 3493916.0 39 230145.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.14576952812835 41.69774060645043 13.277836846154646 36.87865301926657 2 8.536199288409323 45.21525896224265 20.526235193093115 25.722306556254914 3 5.92904375 18.0171625 43.680625 32.37316875 4 8.65231081538852 39.35321794152243 12.559046948808685 39.43542429428037 5 9.584568749999999 42.2847 15.43155625 32.699175000000004 6 8.34966875 46.7934625 18.47616875 26.3807 7 6.22885625 30.16135 38.524081249999995 25.0857125 8 6.4567875 33.01333125 16.43855625 44.091325 9 6.15256875 56.200725 17.98151875 19.665187500000002 10-11 6.09373125 45.23375625 28.480937499999996 20.191575 12-13 5.178662500000001 57.030778125000005 16.621265625 21.16929375 14-15 5.8445687500000005 45.688546875 16.1790375 32.287846875 16-17 5.743609375 55.318578124999995 16.741665625 22.196146875 18-19 17.883921875 31.012365624999997 17.983240625 33.120471875 20-21 27.957803125 31.99073125 17.96605625 22.085409375 22-23 3.75443125 45.296096875 17.479996875 33.469474999999996 24-25 3.626449317063197 44.635938966268334 17.033246413033726 34.704365303634745 26-27 3.6233312500000006 44.801721875 29.041765625 22.533181250000002 28-29 3.670472677915898 43.65583142471313 16.944684956649834 35.729010940721146 30-31 3.630712584321531 33.139962538555665 29.138348030180406 34.0909768469424 32-33 3.691384375 45.366125000000004 21.368321875 29.574168750000002 34-35 3.7139281250000002 44.801087499999994 21.018528125 30.46645625 36-37 3.665978125 44.589215625 21.24588125 30.498925 38-39 3.67749375 44.77518125 21.480431250000002 30.066893750000002 40-41 3.67935625 44.5964125 21.323943749999998 30.4002875 42-43 3.662834375 44.2370125 21.486096874999998 30.614056249999997 44-45 3.695634375 44.53301875 21.458128125 30.313218749999997 46-47 3.694765625 44.502534375 21.256753125 30.545946875000002 48-49 3.688503125 44.251875 21.456803125 30.60281875 50-51 3.6481906250000002 44.40006875 21.55274375 30.398996875 52-53 3.6964343750000004 44.339109375 21.41848125 30.545975 54-55 3.63948125 44.100009375 21.568878124999998 30.69163125 56-57 3.645234375 44.36436875 21.58545625 30.404940625 58-59 3.6970250000000004 44.294925 21.408840625 30.599209375 60-61 3.642740625 44.10720625 21.578187500000002 30.671865625 62-63 3.668559375 44.268184375000004 21.669578125 30.393678125 64-65 3.653584375 44.3154625 21.442959375 30.58799375 66-67 3.62195 44.0704875 21.572468750000002 30.735093749999997 68-69 3.6329468750000005 44.303871875 21.63026875 30.432912499999997 70-71 3.653678125 44.294615625 21.409871875 30.641834375 72-73 3.6358875 44.00121875 21.612368749999998 30.750525 74-75 3.642621875 44.208871875 21.6864625 30.46204375 76-77 3.658228125 44.209909375 21.536493749999998 30.595368750000002 78-79 3.647609375 43.99848125 21.659356250000002 30.694553125000002 80-81 3.663884375 44.226175 21.72265625 30.387284375 82-83 3.70054375 44.2266625 21.518415625 30.554378125 84-85 3.7015781249999997 43.995 21.661056249999998 30.642365625 86-87 3.7338499999999994 44.216303125 21.740365625 30.309481249999997 88-89 3.7835934781716025 44.19833687388316 21.537007584249597 30.481062063695646 90-91 3.8182518828954253 44.02754412022205 21.641039971606606 30.513164025275923 92-93 3.887110421495991 44.184080359954095 21.66745185910913 30.261357359440787 94-95 3.969820492964657 44.16999011870677 21.48260961141705 30.377579776911524 96-97 4.0467970422163235 43.974598459330636 21.58261546277724 30.395989035675807 98-99 4.175008164151795 44.12601075324261 21.64345235026008 30.05552873234551 100-101 4.370128976459587 44.050065331356166 21.35888470818765 30.220920983996596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 6.0 2 4.5 3 9.5 4 18.5 5 20.0 6 23.5 7 36.5 8 48.5 9 58.5 10 71.5 11 93.5 12 138.5 13 232.5 14 441.5 15 777.0 16 1222.5 17 1924.5 18 3210.5 19 5544.5 20 10225.5 21 19748.0 22 37848.5 23 69862.0 24 121954.0 25 201212.5 26 311643.0 27 454040.5 28 621519.0 29 800815.0 30 973075.5 31 1112628.0 32 1202966.0 33 1234018.5 34 1205386.5 35 1129309.0 36 1015374.0 37 880562.5 38 743024.5 39 611549.5 40 493709.0 41 396785.0 42 318022.5 43 255162.0 44 209655.0 45 176350.5 46 150150.0 47 130743.5 48 112919.0 49 93287.0 50 79093.5 51 69260.0 52 61591.0 53 58000.5 54 56128.0 55 53471.0 56 51016.5 57 49229.0 58 47451.5 59 45394.0 60 43134.5 61 40565.0 62 37493.5 63 33985.0 64 30431.0 65 26874.5 66 23217.5 67 19533.0 68 16184.5 69 13090.5 70 10184.0 71 7691.0 72 5781.0 73 4240.5 74 3005.5 75 2105.0 76 1404.0 77 945.5 78 645.5 79 435.5 80 298.5 81 208.5 82 138.0 83 87.5 84 64.5 85 46.5 86 33.5 87 24.5 88 20.0 89 16.5 90 11.5 91 6.0 92 4.5 93 5.5 94 4.0 95 1.5 96 2.0 97 3.0 98 3.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0334625 2 0.04611875 3 0.0 4 1.25E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.843749999999999E-4 26-27 0.0 28-29 2.1875E-5 30-31 5.1875E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.1875E-4 90-91 0.003159375 92-93 0.004609375 94-95 4.3749999999999995E-4 96-97 0.004096875000000001 98-99 0.0010937499999999999 100-101 1.625E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.60027862455203 #Duplication Level Percentage of deduplicated Percentage of total 1 94.77889750934317 84.92215624565009 2 3.7776077820822254 6.769494196176868 3 0.7250360549264614 1.9489029760277086 4 0.2859030569646929 1.0246797425459062 5 0.11717983782229227 0.5249673059028603 6 0.10697661394972265 0.5751080649723773 7 0.045858591592090464 0.2876259808786591 8 0.024430795400036474 0.17512048598901536 9 0.017067046274991726 0.13762908913836433 >10 0.10321535993048311 2.377842239467559 >50 0.016467734417657174 0.9649721356257036 >100 0.0012572082554901816 0.16913564234997894 >500 8.516873984675223E-5 0.04301884399843621 >1k 1.0195536649495288E-5 0.02102929772248745 >5k 7.044764165353849E-6 0.05831775355402013 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00438125 0.0 6.25E-6 2 0.0045375 0.0 6.25E-6 3 0.00463125 0.0 6.25E-6 4 0.00469375 0.0 6.25E-6 5 0.00478125 0.0 6.25E-6 6 0.00493125 0.0 6.25E-6 7 0.00505625 0.0 6.25E-6 8 0.0051125 0.0 6.25E-6 9 0.005175 0.0 6.25E-6 10-11 0.005325 0.0 6.25E-6 12-13 0.0055875000000000005 0.0 1.25E-5 14-15 0.005921875 0.0 1.25E-5 16-17 0.006334375 0.0 1.25E-5 18-19 0.006668749999999999 0.0 1.25E-5 20-21 0.0071937500000000005 0.0 1.25E-5 22-23 0.010615625 6.25E-6 1.25E-5 24-25 0.017290625 6.25E-6 1.25E-5 26-27 0.019209375 9.375000000000001E-6 1.25E-5 28-29 0.02019375 1.25E-5 1.25E-5 30-31 0.02099375 1.25E-5 1.25E-5 32-33 0.021753124999999998 1.875E-5 1.25E-5 34-35 0.02250625 1.875E-5 1.25E-5 36-37 0.023590624999999997 1.875E-5 1.25E-5 38-39 0.024584375 1.875E-5 1.25E-5 40-41 0.02555 1.875E-5 1.25E-5 42-43 0.026828125 2.1875E-5 1.25E-5 44-45 0.02829375 2.5E-5 1.25E-5 46-47 0.030028125 2.5E-5 1.25E-5 48-49 0.032218750000000004 2.5E-5 1.25E-5 50-51 0.034493750000000004 2.5E-5 1.25E-5 52-53 0.037346875 2.5E-5 1.25E-5 54-55 0.040743749999999995 2.5E-5 1.25E-5 56-57 0.044875 2.5E-5 1.5625E-5 58-59 0.049828125 2.5E-5 2.5E-5 60-61 0.0556375 2.8125E-5 2.5E-5 62-63 0.063071875 3.125E-5 2.5E-5 64-65 0.07236875 3.125E-5 2.5E-5 66-67 0.083746875 3.125E-5 2.5E-5 68-69 0.098321875 3.125E-5 2.5E-5 70-71 0.115853125 3.125E-5 2.5E-5 72-73 0.13741562499999999 3.125E-5 2.5E-5 74-75 0.16488125 3.125E-5 2.5E-5 76-77 0.19979375 3.125E-5 2.8125E-5 78-79 0.244565625 3.125E-5 3.125E-5 80-81 0.30081875 3.125E-5 3.125E-5 82-83 0.3741 3.125E-5 3.125E-5 84-85 0.46714687499999996 3.125E-5 3.125E-5 86-87 0.58356875 3.125E-5 3.125E-5 88-89 0.7290593750000001 3.125E-5 3.125E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAATC 444380 0.0 79.22998 2 AATAAAT 454710 0.0 76.66018 1 TAAATCA 468635 0.0 75.720795 3 AATCATA 506605 0.0 71.231575 5 TCATAAA 509830 0.0 70.87322 7 AAATCAT 508350 0.0 69.838715 4 ATCATAA 527250 0.0 69.06945 6 CATAAAC 555725 0.0 64.94062 8 ATAAACA 623045 0.0 57.939034 9 ATGAATC 2470 0.0 55.40522 2 GTCCCCA 1930 0.0 44.826088 1 TGCCCGT 21620 0.0 43.599728 1 GTCCCCC 1135 0.0 42.718998 1 AGAAATC 4565 0.0 41.532425 2 CCCCGTT 1815 0.0 39.023514 1 ACAAACG 493710 0.0 37.70437 12-13 GAATCAT 3710 0.0 37.38451 4 TTGCCCG 12230 0.0 36.458023 1 AAACGGG 505030 0.0 36.430363 14-15 GATAAAT 4905 0.0 36.342014 1 >>END_MODULE