##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 2212_lane2_GCCAAT_L002_R1_002.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.1846519375 31.0 28.0 34.0 16.0 34.0 2 30.0790166875 31.0 30.0 34.0 25.0 34.0 3 30.785475625 31.0 30.0 34.0 26.0 34.0 4 33.588304 35.0 33.0 37.0 28.0 37.0 5 34.2706048125 35.0 35.0 37.0 30.0 37.0 6 35.084873375 37.0 35.0 37.0 32.0 37.0 7 35.3094940625 37.0 35.0 37.0 33.0 37.0 8 35.4124281875 37.0 35.0 37.0 33.0 37.0 9 37.0755224375 39.0 37.0 39.0 33.0 39.0 10-11 37.11040971875 39.0 37.0 39.0 33.0 39.0 12-13 37.138672937500004 39.0 37.0 39.0 33.0 39.0 14-15 38.396418656250006 40.0 38.0 41.0 33.5 41.0 16-17 38.305206156249994 40.0 38.0 41.0 33.0 41.0 18-19 38.314787875 40.0 38.0 41.0 33.0 41.0 20-21 38.29168265625 40.0 38.0 41.0 33.0 41.0 22-23 38.2993440625 40.0 38.0 41.0 34.0 41.0 24-25 38.26054834375 40.0 38.0 41.0 34.0 41.0 26-27 38.12466665625 40.0 38.0 41.0 33.0 41.0 28-29 37.821103156250004 40.0 37.0 41.0 32.5 41.0 30-31 37.936906343749996 40.0 37.0 41.0 33.0 41.0 32-33 37.670461187499996 40.0 37.0 41.0 32.0 41.0 34-35 37.5292685625 40.0 37.0 41.0 31.5 41.0 36-37 37.439046125000004 40.0 37.0 41.0 31.0 41.0 38-39 37.3937898125 40.0 37.0 41.0 31.0 41.0 40-41 37.32082553125 40.0 37.0 41.0 31.0 41.0 42-43 37.28214603125 40.0 37.0 41.0 31.0 41.0 44-45 37.1623650625 40.0 37.0 41.0 31.0 41.0 46-47 37.07259546875 40.0 36.0 41.0 31.0 41.0 48-49 36.97341184375 40.0 36.0 41.0 30.5 41.0 50-51 36.8703419375 40.0 36.0 41.0 30.0 41.0 52-53 36.735078125 39.5 36.0 41.0 30.0 41.0 54-55 36.58173078125 39.0 36.0 41.0 30.0 41.0 56-57 36.41882446875 39.0 35.0 41.0 29.0 41.0 58-59 36.2462183125 39.0 35.0 40.5 29.0 41.0 60-61 36.028144749999996 39.0 35.0 40.0 28.5 41.0 62-63 35.74923628125 38.0 35.0 40.0 28.0 41.0 64-65 35.3658736875 37.5 34.0 40.0 28.0 41.0 66-67 34.97484903125 37.0 34.0 39.0 28.0 41.0 68-69 34.552717187499994 36.5 34.0 39.0 28.0 40.0 70-71 34.09070428125 36.0 34.0 38.5 28.0 40.0 72-73 33.629371093749995 35.5 33.0 37.0 26.5 39.0 74-75 33.189720593749996 35.0 33.0 37.0 26.0 39.0 76-77 30.535586000000002 32.5 29.5 34.5 23.0 36.5 78-79 32.32248365625 34.5 32.0 36.0 26.0 37.0 80-81 32.405240875 35.0 33.0 35.5 26.0 37.0 82-83 32.23753734375 35.0 33.0 35.0 26.0 36.0 84-85 32.0513375 35.0 33.0 35.0 26.0 36.0 86-87 31.884891250000003 35.0 33.0 35.0 26.0 36.0 88-89 31.7281411875 35.0 32.5 35.0 26.0 35.5 90-91 31.59622109375 35.0 32.0 35.0 25.0 35.0 92-93 31.45019209375 35.0 32.0 35.0 25.0 35.0 94-95 31.2439336875 34.0 32.0 35.0 25.0 35.0 96-97 31.12361846875 34.0 32.0 35.0 24.5 35.0 98-99 30.937822375 34.0 32.0 35.0 23.5 35.0 100-101 30.06596846875 34.0 31.0 35.0 17.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1307 1 1.8698581258158775 1307 2 1.5281428716291927 1307 3 0.6005380418973338 1307 4 0.49111970539101435 1307 5 1.140388518034733 1307 6 0.4530690851715917 1307 7 0.24988072839906295 1307 8 0.11608186789841568 1307 9 0.02021317565878178 1307 10-11 0.037879510743529465 1307 12-13 0.15879568569932445 1307 14-15 0.11258610111323009 1307 16-17 0.1745182436247319 1307 18-19 0.16519535780797412 1307 20-21 0.31029405015902256 1307 22-23 0.2975176097340224 1307 24-25 0.29048722408250427 1307 26-27 0.29572992612849447 1307 28-29 0.2690238392138795 1307 30-31 0.3141538175301264 1307 32-33 0.3449568923645785 1307 34-35 0.3841255155146328 1307 36-37 0.44834180433508664 1307 38-39 0.27647790565985275 1307 40-41 0.3505884989577268 1307 42-43 0.42319880367152507 1307 44-45 0.37919603903110755 1307 46-47 0.4356658270066305 1307 48-49 0.5488039670360934 1307 50-51 0.5656886630234794 1307 52-53 0.5062507306704944 1307 54-55 0.5389784877435986 1307 56-57 0.4609791348270278 1307 58-59 0.49736322732720595 1307 60-61 0.5158196294139259 1307 62-63 0.43919340232437065 1307 64-65 0.5471838879912596 1307 66-67 0.46098209418035196 1307 68-69 0.5165227239070731 1307 70-71 0.5521097993005171 1307 72-73 0.6081228183392611 1307 74-75 0.46864557518925665 1307 76-77 0.4132790279929708 1307 78-79 0.4448384578279274 1307 80-81 0.40145206824613666 1307 82-83 0.4637406262251105 1307 84-85 0.4831906376369801 1307 86-87 0.49834107383654924 1307 88-89 0.5339010202765913 1307 90-91 0.5411991757864705 1307 92-93 0.5278846989222146 1307 94-95 0.561396903303951 1307 96-97 0.5390267071271886 1307 98-99 0.5400412530022862 1307 100-101 0.5288287956398356 1306 1 0.3317850291312574 1306 2 0.9689829606755609 1306 3 0.46259929209906403 1306 4 -1.2232617611113739 1306 5 0.4535976002483082 1306 6 0.23543379906426054 1306 7 0.11803246240059195 1306 8 0.07780800303459756 1306 9 0.11975827201266043 1306 10-11 0.19203105221432537 1306 12-13 0.18412693406207126 1306 14-15 0.16086029424047155 1306 16-17 0.18951429018599697 1306 18-19 0.14729072164798396 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86-87 0.30773727437960474 1306 88-89 0.2759528897482859 1306 90-91 0.23488800745926497 1306 92-93 0.19366918747739348 1306 94-95 0.15996371870628678 1306 96-97 0.23491509916071251 1306 98-99 0.22244203225636028 1306 100-101 0.11421009483403921 1305 1 0.07756675855776507 1305 2 0.9286029703567138 1305 3 0.4596703597893175 1305 4 -1.1635738564983455 1305 5 0.47905897412916687 1305 6 0.17704042629248562 1305 7 0.12299197305979703 1305 8 0.11667735603706575 1305 9 0.18822600345915674 1305 10-11 0.15066206281035477 1305 12-13 0.15575716914292315 1305 14-15 0.12038421649986475 1305 16-17 0.15902704940940282 1305 18-19 0.14983238075870275 1305 20-21 0.1318071302401549 1305 22-23 0.1401870597350907 1305 24-25 0.16242994619860696 1305 26-27 0.17533693303200693 1305 28-29 0.2591871435745716 1305 30-31 0.20055840491322385 1305 32-33 0.19580468080751245 1305 34-35 0.23586051348316772 1305 36-37 0.271078154731768 1305 38-39 0.24416871908040605 1305 40-41 0.2623364421506196 1305 42-43 0.2673078152486852 1305 44-45 0.33409813843556435 1305 46-47 0.3040723305556128 1305 48-49 0.26150918444062654 1305 50-51 0.26486364960438635 1305 52-53 0.2655184782877029 1305 54-55 0.26757892606293865 1305 56-57 0.2753911853031141 1305 58-59 0.29401610999189387 1305 60-61 0.2893030514126238 1305 62-63 0.30408785133408145 1305 64-65 0.3290849798119524 1305 66-67 0.31529560037908766 1305 68-69 0.27533533753939565 1305 70-71 0.2932122267542496 1305 72-73 0.2234879344791807 1305 74-75 0.20814834522000325 1305 76-77 0.017302437585190233 1305 78-79 0.28507812198263593 1305 80-81 0.27847212539100497 1305 82-83 0.2647938235645597 1305 84-85 0.25632157390363375 1305 86-87 0.2803372519620311 1305 88-89 0.26312590569719063 1305 90-91 0.25709974381704015 1305 92-93 0.2408474623507395 1305 94-95 0.27122296701532633 1305 96-97 0.26951275201017566 1305 98-99 0.2887503649444554 1305 100-101 0.20800462246433327 1304 1 1.3972664776597874 1304 2 1.4193891003867343 1304 3 0.3500444118820738 1304 4 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0.15380324996635153 1304 66-67 0.18728949957898067 1304 68-69 0.22970971489206704 1304 70-71 0.19565773799807573 1304 72-73 0.22141300908270267 1304 74-75 0.17521226613700236 1304 76-77 -0.09456456970436733 1304 78-79 0.13006165563479044 1304 80-81 0.142445798062937 1304 82-83 0.19342762537614533 1304 84-85 0.14322259738889898 1304 86-87 0.1917103840502392 1304 88-89 0.29662204374323053 1304 90-91 0.306813804449785 1304 92-93 0.3242496718775989 1304 94-95 0.24443818145007867 1304 96-97 0.26296593713476213 1304 98-99 0.2954566600039428 1304 100-101 0.15391155959786573 1216 1 1.006942469279327 1216 2 0.6318686858646494 1216 3 0.4243186905097396 1216 4 0.9759011133868469 1216 5 0.37931160965126764 1216 6 0.2263540957606125 1216 7 0.043255527261791826 1216 8 0.07457849219466794 1216 9 0.03056604455209566 1216 10-11 0.05323394450488905 1216 12-13 0.018543871700494208 1216 14-15 0.016171480874724864 1216 16-17 0.06408630531777249 1216 18-19 0.022015738875317936 1216 20-21 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0.353468301917367 1214 36-37 0.451975553483976 1214 38-39 0.49058583660303157 1214 40-41 0.5134037817595143 1214 42-43 0.48256465614618804 1214 44-45 0.46331147139015627 1214 46-47 0.5054668027078577 1214 48-49 0.49585490643870855 1214 50-51 0.5105596056855006 1214 52-53 0.49118941399355265 1214 54-55 0.4881169664546192 1214 56-57 0.4844033072785052 1214 58-59 0.5778571716285583 1214 60-61 0.4946956579471333 1214 62-63 0.5737566021238223 1214 64-65 0.594645397163859 1214 66-67 0.5514451846934776 1214 68-69 0.49274250208789283 1214 70-71 0.5208565319676026 1214 72-73 0.5410879442574057 1214 74-75 0.5036654680702313 1214 76-77 0.6396160496469605 1214 78-79 0.47951167526413485 1214 80-81 0.5008733502963807 1214 82-83 0.47691093517883587 1214 84-85 0.40518664474021193 1214 86-87 0.4117937111840675 1214 88-89 0.4478153268005407 1214 90-91 0.40278517261098656 1214 92-93 0.42220736347028165 1214 94-95 0.45264080354647973 1214 96-97 0.5099593552992481 1214 98-99 0.4858723047922169 1214 100-101 0.4632732599153364 1215 1 0.9037859464106184 1215 2 1.2189950582614664 1215 3 0.9595677749309992 1215 4 0.41160903508452407 1215 5 1.0650916026015196 1215 6 0.4911983786516103 1215 7 0.29999228057182137 1215 8 0.17948130289117614 1215 9 0.17496354848218942 1215 10-11 0.21327037443781904 1215 12-13 0.19081959887637368 1215 14-15 0.13192547803057408 1215 16-17 0.1732760628791894 1215 18-19 0.19768841039165608 1215 20-21 0.2192515691701189 1215 22-23 0.22198798723010782 1215 24-25 0.20556109991499483 1215 26-27 0.28138366942710746 1215 28-29 0.2669396102511499 1215 30-31 0.24153694981869478 1215 32-33 0.33393277921074116 1215 34-35 0.29941209748503894 1215 36-37 0.40889109908625443 1215 38-39 0.37711867486823536 1215 40-41 0.39926237936465014 1215 42-43 0.3971800343211953 1215 44-45 0.3832360932455927 1215 46-47 0.37550300493570177 1215 48-49 0.46857007877976287 1215 50-51 0.4141796329073415 1215 52-53 0.3095752549716053 1215 54-55 0.4493080552892721 1215 56-57 0.4415153438113464 1215 58-59 0.40175742249854096 1215 60-61 0.4779397741630902 1215 62-63 0.42972314366260633 1215 64-65 0.44737449143103447 1215 66-67 0.5063996169463252 1215 68-69 0.4586571012842171 1215 70-71 0.31749414833554823 1215 72-73 0.44933646225170065 1215 74-75 0.46666410417051196 1215 76-77 0.5612595771631135 1215 78-79 0.473465128387744 1215 80-81 0.4589276889473055 1215 82-83 0.5139161708088693 1215 84-85 0.49927554394729157 1215 86-87 0.5076053123167625 1215 88-89 0.4879024537391281 1215 90-91 0.48367538285090106 1215 92-93 0.4709150595248026 1215 94-95 0.4860094265101935 1215 96-97 0.4908910665435151 1215 98-99 0.4732029874421855 1215 100-101 0.3803743040737153 1212 1 1.4289311456818936 1212 2 1.5157120804041995 1212 3 1.0747832992497912 1212 4 2.164416442780947 1212 5 1.5442841837026862 1212 6 0.6566971506402979 1212 7 0.3207643214914313 1212 8 0.2059320150706938 1212 9 0.20011667218658147 1212 10-11 0.08911838117980153 1212 12-13 0.16614359478757734 1212 14-15 0.17300752341014203 1212 16-17 0.25340669148362593 1212 18-19 0.2556128241994884 1212 20-21 0.24438553505355998 1212 22-23 0.25829812781634587 1212 24-25 0.25041475006270986 1212 26-27 0.27174582153680404 1212 28-29 0.33715653731129436 1212 30-31 0.2577931626750427 1212 32-33 0.2505787971597613 1212 34-35 0.32665798229084686 1212 36-37 0.35386043556126623 1212 38-39 0.38473220775470196 1212 40-41 0.3922636473375931 1212 42-43 0.3429559813392231 1212 44-45 0.32509496793917947 1212 46-47 0.31236206559355395 1212 48-49 0.3675416685519508 1212 50-51 0.3544984204398034 1212 52-53 0.4264788424568948 1212 54-55 0.43180602649648847 1212 56-57 0.465892413247353 1212 58-59 0.43272590385614507 1212 60-61 0.48323744843850136 1212 62-63 0.49914524032163854 1212 64-65 0.49012591316662935 1212 66-67 0.5294891484646556 1212 68-69 0.44914188076081984 1212 70-71 0.47477528148662884 1212 72-73 0.49289876842001235 1212 74-75 0.5043625038105546 1212 76-77 0.6584674900373741 1212 78-79 0.5069944453637731 1212 80-81 0.5232029897792572 1212 82-83 0.45878070363255574 1212 84-85 0.4948748185402323 1212 86-87 0.5168636329025844 1212 88-89 0.4778755272155486 1212 90-91 0.45743988575693706 1212 92-93 0.46959682127118896 1212 94-95 0.49379764721272323 1212 96-97 0.5340166464884284 1212 98-99 0.5194320206598562 1212 100-101 0.5293042679762756 1213 1 1.2853659767049699 1213 2 1.1023483066278175 1213 3 0.7982574501622182 1213 4 1.491400232675133 1213 5 1.3325354020276876 1213 6 0.5637282355715953 1213 7 0.26008762002157937 1213 8 0.23675303692803595 1213 9 0.17278531455116308 1213 10-11 0.1254865916772232 1213 12-13 0.14991332696113346 1213 14-15 0.1265895703874662 1213 16-17 0.21860348530468343 1213 18-19 0.17616341031946803 1213 20-21 0.11152321038351687 1213 22-23 0.1682197542592334 1213 24-25 0.14210371533086175 1213 26-27 0.16280240471511576 1213 28-29 0.17516660427273223 1213 30-31 0.2058514171671959 1213 32-33 0.2517513912763647 1213 34-35 0.26119454761116145 1213 36-37 0.2920417920925189 1213 38-39 0.2877291625909706 1213 40-41 0.26028359889463104 1213 42-43 0.26680851086656787 1213 44-45 0.2939618318092698 1213 46-47 0.26609505898554175 1213 48-49 0.2579227470363321 1213 50-51 0.28422638289523405 1213 52-53 0.23273397493113634 1213 54-55 0.3375023256806884 1213 56-57 0.31724859136383543 1213 58-59 0.33917610062707126 1213 60-61 0.2779354278920181 1213 62-63 0.30884627124842723 1213 64-65 0.30761144627280146 1213 66-67 0.3144736672116508 1213 68-69 0.24642090692717034 1213 70-71 0.27542643545439205 1213 72-73 0.31599751840996504 1213 74-75 0.3353170944078627 1213 76-77 0.5979027827757832 1213 78-79 0.3704510150681841 1213 80-81 0.3281973534328486 1213 82-83 0.3516110410063362 1213 84-85 0.31845408423475874 1213 86-87 0.32724322355840485 1213 88-89 0.3681425049218383 1213 90-91 0.3997713660211808 1213 92-93 0.39195208236830936 1213 94-95 0.38751293524137154 1213 96-97 0.4005080791095885 1213 98-99 0.3854857772692242 1213 100-101 0.4680177460848931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 522.0 3 107.0 4 147.0 5 271.0 6 697.0 7 1732.0 8 3852.0 9 6465.0 10 9291.0 11 13260.0 12 20012.0 13 33434.0 14 37882.0 15 42603.0 16 47319.0 17 47627.0 18 47840.0 19 49691.0 20 51950.0 21 54310.0 22 57482.0 23 65807.0 24 80088.0 25 103541.0 26 135683.0 27 175850.0 28 224291.0 29 288494.0 30 372708.0 31 477935.0 32 615924.0 33 805477.0 34 1088567.0 35 1535088.0 36 2252841.0 37 3598594.0 38 3426855.0 39 225714.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.185709866718147 41.727599038652215 13.447834929453068 36.63885616517658 2 8.487081802330293 45.330499081148076 20.750003501341013 25.432415615180616 3 5.7869375000000005 18.07413125 43.872299999999996 32.26663125 4 8.77028125 39.4857125 12.44216875 39.3018375 5 9.639312274570171 42.430876519297826 15.43089089430681 32.498920311825195 6 8.34021253095639 46.900336051512234 18.412820357691608 26.34663105983977 7 6.219289832233687 30.134586300469863 38.6434519912945 25.00267187600195 8 6.434693750000001 32.973124999999996 16.382512499999997 44.20966875 9 6.1185937500000005 56.31533125 17.93318125 19.63289375 10-11 6.06450625 45.31428125 28.5061625 20.11505 12-13 5.1656812500000004 57.1577375 16.544421875 21.132159375 14-15 5.8206875 45.74011875 16.1271375 32.31205625 16-17 5.71955 55.46314375 16.657659375 22.159646875 18-19 17.980171875 30.945662499999997 17.93925 33.134915625 20-21 28.123150000000003 31.931553125 17.885340625 22.059956250000003 22-23 3.73501875 45.363196875 17.432596875 33.469187500000004 24-25 3.5985647491029686 44.679506049691284 16.984044990028117 34.73788421117764 26-27 3.5876625000000004 44.870653125 29.082134375000003 22.45955 28-29 3.6428937500000003 43.6917 16.8864625 35.778943749999996 30-31 3.6036985671983484 33.108389747100105 29.178043329247995 34.109868356453546 32-33 3.6629939789371235 45.41767783860487 21.339707583731723 29.579620598726287 34-35 3.685845265769853 44.8488878537171 21.009355203570063 30.455911676942982 36-37 3.6417702515537735 44.624772496587454 21.223327724915876 30.510129526942904 38-39 3.6451649566588396 44.80103735158845 21.489976656526753 30.063821035225963 40-41 3.653157263665969 44.64079257225388 21.314532488201763 30.391517675878394 42-43 3.636721875 44.267934375 21.474271875 30.621071875 44-45 3.6706573134317346 44.570251746653405 21.439189092693983 30.319901847220876 46-47 3.6673709260861367 44.546627061136945 21.227546418433345 30.558455594343574 48-49 3.66125625 44.2913375 21.429353125 30.618053125 50-51 3.629542521977639 44.4161405918892 21.545917648268397 30.408399237864757 52-53 3.6677968749999996 44.378890625 21.402537499999998 30.550775 54-55 3.6135624999999996 44.109365625 21.57661875 30.700453125 56-57 3.6253895392816156 44.36798120698943 21.566434006119128 30.440195247609825 58-59 3.6736375000000003 44.3231375 21.387978125 30.615246875 60-61 3.61253125 44.131715625 21.559234375 30.696518750000003 62-63 3.638625 44.32675625 21.65935 30.37526875 64-65 3.62111875 44.32736875 21.4388625 30.612650000000002 66-67 3.598134375 44.126659375 21.562203125 30.713003125 68-69 3.6052562500000005 44.332806250000004 21.618515625 30.443421875 70-71 3.626875 44.32501875 21.41009375 30.638012500000002 72-73 3.608346875 44.042659375 21.590065624999998 30.758928125 74-75 3.613225 44.23186875 21.6859125 30.46899375 76-77 3.6393375 44.26123125 21.5233625 30.57606875 78-79 3.6218562500000004 44.038315625 21.654803125 30.685025 80-81 3.6339812499999997 44.275940625 21.704428125 30.38565 82-83 3.67295625 44.272984375 21.499415625 30.55464375 84-85 3.6721093750000002 44.04025 21.63316875 30.654471875 86-87 3.70240625 44.2291875 21.752071875000002 30.316334374999997 88-89 3.7546935309822267 44.23412668525693 21.533181056932392 30.47799872682845 90-91 3.7953052264105813 44.054855382225306 21.636183016529756 30.513656374834362 92-93 3.8521991866378 44.217558683922995 21.65661628961462 30.273625839824582 94-95 3.9425854555839015 44.19160345468267 21.45167465287381 30.414136436859618 96-97 4.020876437088737 43.99955722747394 21.579260344870047 30.400305990567272 98-99 4.145951762530845 44.172198288934986 21.627277136799165 30.054572811735003 100-101 4.340432569186541 44.096757517047976 21.352023474875445 30.21078643889003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 2.0 2 3.5 3 6.0 4 7.5 5 8.5 6 13.0 7 23.0 8 26.5 9 35.0 10 54.0 11 74.5 12 135.0 13 251.5 14 425.0 15 696.5 16 1151.5 17 1911.5 18 3171.0 19 5579.0 20 10337.5 21 19771.5 22 37727.0 23 69626.0 24 122555.0 25 202509.5 26 312390.5 27 454398.5 28 622796.0 29 802733.0 30 974876.0 31 1114588.5 32 1205330.5 33 1237583.0 34 1209201.0 35 1130649.5 36 1016625.0 37 883402.0 38 745344.0 39 612262.0 40 493125.5 41 395291.0 42 316115.5 43 252943.0 44 207570.0 45 174527.5 46 147632.5 47 127806.0 48 110473.0 49 91735.5 50 77467.5 51 68101.5 52 60920.5 53 57676.0 54 56039.0 55 53231.5 56 50839.5 57 48955.0 58 47277.0 59 45552.0 60 43207.0 61 40205.0 62 36835.5 63 33430.0 64 30052.5 65 26606.0 66 22917.0 67 19237.5 68 15927.0 69 12901.5 70 10213.5 71 7811.5 72 5739.5 73 4127.5 74 2965.5 75 2043.5 76 1352.5 77 938.5 78 629.0 79 410.5 80 287.0 81 192.5 82 129.0 83 86.0 84 56.5 85 38.0 86 25.0 87 20.0 88 15.0 89 8.0 90 6.5 91 6.0 92 5.0 93 1.5 94 0.5 95 1.5 96 1.0 97 0.5 98 2.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025775 2 0.0383 3 0.0 4 0.0 5 6.25E-5 6 4.5E-4 7 3.7500000000000003E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.25E-5 26-27 0.0 28-29 0.0 30-31 0.0031687499999999997 32-33 6.2499999999999995E-6 34-35 0.0031781249999999995 36-37 0.0015 38-39 1.53125E-4 40-41 2.84375E-4 42-43 0.0 44-45 2.84375E-4 46-47 0.0013375 48-49 0.0 50-51 0.00134375 52-53 0.0 54-55 0.0 56-57 5.625E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.00365625 90-91 0.0034312500000000003 92-93 0.008415625 94-95 0.025549999999999996 96-97 0.0050875 98-99 0.003434375 100-101 5.34375E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.78771813340616 #Duplication Level Percentage of deduplicated Percentage of total 1 94.54813368036721 84.89261176932418 2 4.103954006616329 7.369693311570597 3 0.7096746702880165 1.911602077867151 4 0.22617840879753065 0.8123217286789997 5 0.11694609014267225 0.5250161289267083 6 0.07194912164225369 0.3876088472376496 7 0.04377723392746303 0.27514605583774654 8 0.029606783299849393 0.21266604110109713 9 0.024775268455601632 0.20020633386939254 >10 0.11012595763948889 2.3265804424305685 >50 0.013223792882237394 0.7358984648377656 >100 0.0015779077185196198 0.24987000041139565 >500 5.509859984996106E-5 0.028796944611510316 >1k 1.4929705596087894E-5 0.016809922641368505 >5k 7.049917350146402E-6 0.055171930653895467 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00411875 0.0 0.0 2 0.00428125 0.0 0.0 3 0.00438125 0.0 6.25E-6 4 0.00446875 0.0 6.25E-6 5 0.00454375 0.0 6.25E-6 6 0.00465625 0.0 6.25E-6 7 0.00483125 6.25E-6 6.25E-6 8 0.00490625 6.25E-6 1.25E-5 9 0.005025 6.25E-6 1.25E-5 10-11 0.005140625 6.25E-6 1.25E-5 12-13 0.005428125000000001 6.25E-6 1.25E-5 14-15 0.005809375 6.25E-6 1.25E-5 16-17 0.00618125 6.25E-6 1.25E-5 18-19 0.00644375 6.25E-6 1.25E-5 20-21 0.006984375 6.25E-6 1.25E-5 22-23 0.01026875 6.25E-6 1.25E-5 24-25 0.016774999999999998 6.25E-6 1.25E-5 26-27 0.0186875 1.25E-5 1.25E-5 28-29 0.01965625 1.25E-5 1.25E-5 30-31 0.0204625 1.25E-5 1.25E-5 32-33 0.02119375 1.25E-5 1.25E-5 34-35 0.02205625 1.25E-5 1.25E-5 36-37 0.023115625 1.5625E-5 1.25E-5 38-39 0.0241875 1.875E-5 1.25E-5 40-41 0.0253625 1.875E-5 1.25E-5 42-43 0.026615625 1.875E-5 1.5625E-5 44-45 0.028103125 1.875E-5 1.875E-5 46-47 0.029953125 1.875E-5 1.875E-5 48-49 0.031878125 2.1875E-5 1.875E-5 50-51 0.034334375 2.5E-5 1.875E-5 52-53 0.03728125 2.5E-5 1.875E-5 54-55 0.04081875 2.5E-5 1.875E-5 56-57 0.04485 2.5E-5 1.875E-5 58-59 0.04979375 2.5E-5 1.875E-5 60-61 0.05584375 2.8125E-5 1.875E-5 62-63 0.06311875 3.125E-5 1.875E-5 64-65 0.072165625 3.125E-5 1.875E-5 66-67 0.08305625 3.125E-5 1.875E-5 68-69 0.096553125 3.125E-5 1.875E-5 70-71 0.113803125 3.125E-5 1.875E-5 72-73 0.135965625 3.125E-5 1.875E-5 74-75 0.16375625 3.125E-5 1.875E-5 76-77 0.198784375 3.125E-5 1.875E-5 78-79 0.243259375 3.75E-5 1.875E-5 80-81 0.301240625 3.75E-5 1.875E-5 82-83 0.373609375 3.75E-5 1.875E-5 84-85 0.465271875 3.75E-5 1.875E-5 86-87 0.582140625 3.75E-5 1.875E-5 88-89 0.7298625 3.75E-5 1.875E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAATC 446870 0.0 79.11489 2 AATAAAT 454710 0.0 76.71872 1 TAAATCA 471205 0.0 75.64591 3 AATCATA 510190 0.0 71.431496 5 TCATAAA 513940 0.0 71.19127 7 AAATCAT 511015 0.0 69.96194 4 ATCATAA 531605 0.0 69.29376 6 CATAAAC 560285 0.0 65.20422 8 ATAAACA 629680 0.0 58.01978 9 ATGAATC 2885 0.0 55.995472 2 GATAAAT 5725 0.0 45.907486 1 AGAAATC 5150 0.0 45.39183 2 GTAAATC 2420 0.0 45.354088 2 GTGCAAC 1690 0.0 44.983486 2 TGCCCGT 20865 0.0 44.280422 1 CGTGCAA 1650 0.0 43.78179 1 GAATCAT 3890 0.0 43.22365 4 CCCCGTT 2045 0.0 41.367622 1 GTCCCCC 1130 0.0 41.217487 1 GTCCCCA 1820 0.0 39.953415 1 >>END_MODULE