##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2212_lane2_GCCAAT_L002_R1_004.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16000000
Sequences flagged as poor quality	0
Sequence length	101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.6787188125	31.0	28.0	34.0	16.0	34.0
2	29.5504329375	31.0	28.0	34.0	16.0	34.0
3	30.605646125	31.0	30.0	34.0	25.0	34.0
4	33.730462	35.0	35.0	37.0	28.0	37.0
5	33.7868365	35.0	35.0	37.0	28.0	37.0
6	34.920216375	37.0	35.0	37.0	32.0	37.0
7	35.328867	37.0	35.0	37.0	33.0	37.0
8	35.4726225625	37.0	35.0	37.0	33.0	37.0
9	37.2189341875	39.0	37.0	39.0	34.0	39.0
10-11	37.24772503125	39.0	37.0	39.0	34.0	39.0
12-13	37.330246375	39.0	37.0	39.0	34.0	39.0
14-15	38.67410659375	40.0	38.0	41.0	34.0	41.0
16-17	38.616692093750004	40.0	38.0	41.0	34.0	41.0
18-19	38.64029403125	40.0	38.0	41.0	34.0	41.0
20-21	38.66377596875	40.0	38.0	41.0	34.0	41.0
22-23	38.64017978125	40.0	38.0	41.0	34.0	41.0
24-25	38.6038721875	40.0	38.0	41.0	34.0	41.0
26-27	38.47193246875	40.0	38.0	41.0	34.0	41.0
28-29	38.15559771875	40.0	38.0	41.0	33.5	41.0
30-31	38.27334890625	40.0	38.0	41.0	34.0	41.0
32-33	38.005953937499996	40.0	38.0	41.0	33.0	41.0
34-35	37.91158715625	40.0	38.0	41.0	33.0	41.0
36-37	37.8480414375	40.0	38.0	41.0	33.0	41.0
38-39	37.7834203125	40.0	38.0	41.0	33.0	41.0
40-41	37.71023646875	40.0	37.0	41.0	32.5	41.0
42-43	37.64664953125	40.0	37.0	41.0	32.0	41.0
44-45	37.55636453125	40.0	37.0	41.0	32.0	41.0
46-47	37.46309425	40.0	37.0	41.0	31.0	41.0
48-49	37.3790156875	40.0	37.0	41.0	31.0	41.0
50-51	37.2792633125	40.0	37.0	41.0	31.0	41.0
52-53	37.154536875	40.0	37.0	41.0	31.0	41.0
54-55	36.9782866875	40.0	36.0	41.0	31.0	41.0
56-57	36.803113906250005	40.0	36.0	41.0	30.0	41.0
58-59	36.67087684375	39.5	36.0	41.0	30.0	41.0
60-61	36.4379368125	39.0	35.0	41.0	30.0	41.0
62-63	36.124956499999996	39.0	35.0	40.5	29.5	41.0
64-65	35.782095562500004	38.5	35.0	40.0	29.0	41.0
66-67	35.336023749999995	37.0	35.0	39.5	29.0	41.0
68-69	34.88823240625	37.0	34.0	39.0	29.0	40.5
70-71	34.41942925	36.0	34.0	39.0	28.5	40.0
72-73	33.9572095625	36.0	34.0	37.0	28.0	39.0
74-75	33.50555828125	35.0	33.5	37.0	28.0	39.0
76-77	31.45999571875	33.5	31.0	35.5	25.0	36.5
78-79	32.63474290625	35.0	33.0	36.0	26.5	37.0
80-81	32.6118550625	35.0	33.0	36.0	27.0	37.0
82-83	32.40920415625	35.0	33.0	35.0	27.0	36.5
84-85	32.225221625	35.0	33.0	35.0	27.0	36.0
86-87	32.08057459375	35.0	33.0	35.0	26.5	36.0
88-89	31.907240218749997	35.0	33.0	35.0	26.0	36.0
90-91	31.7928983125	35.0	33.0	35.0	26.0	35.0
92-93	31.64108778125	35.0	33.0	35.0	25.5	35.0
94-95	31.51810553125	35.0	33.0	35.0	25.0	35.0
96-97	31.34232221875	35.0	33.0	35.0	25.0	35.0
98-99	31.14867796875	35.0	33.0	35.0	24.5	35.0
100-101	30.42079146875	34.5	32.0	35.0	20.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	-1.9447225008054794
2201	2	-1.749649940095626
2201	3	-1.5417856085854709
2201	4	-0.9463261686554034
2201	5	-1.3668013599685551
2201	6	-0.5509661291234593
2201	7	-0.1999849825849651
2201	8	-0.1565931955928761
2201	9	-0.12342481207992506
2201	10-11	-0.03290057688992931
2201	12-13	-0.058232743321930514
2201	14-15	-0.08989323472610522
2201	16-17	-0.13305567433701526
2201	18-19	-0.11816695892796503
2201	20-21	-0.23052321097006967
2201	22-23	-0.2344382195350363
2201	24-25	-0.18654232112793778
2201	26-27	-0.21839689867449863
2201	28-29	-0.24260166364590674
2201	30-31	-0.18857079988510606
2201	32-33	-0.17493150465426766
2201	34-35	-0.17261670920328953
2201	36-37	-0.22280811435622638
2201	38-39	-0.25430982761075427
2201	40-41	-0.2188685120575542
2201	42-43	-0.22935908101936064
2201	44-45	-0.23959936766708267
2201	46-47	-0.2559777266618468
2201	48-49	-0.32772758687782755
2201	50-51	-0.28392972903386493
2201	52-53	-0.2143564820610422
2201	54-55	-1.512254565297745
2201	56-57	-1.3819126366476837
2201	58-59	-0.5582002372120485
2201	60-61	-0.3422717680973335
2201	62-63	-0.3279537167497537
2201	64-65	-0.26171509328063536
2201	66-67	-0.29592947540261605
2201	68-69	-0.28137206938213666
2201	70-71	-0.21892859292210431
2201	72-73	-0.260044847537074
2201	74-75	-0.27237588263512436
2201	76-77	-0.45260913849791606
2201	78-79	-0.28339644434094424
2201	80-81	-0.20231676701809675
2201	82-83	-0.27916266330088035
2201	84-85	-0.2994054827067778
2201	86-87	-0.2566467762951099
2201	88-89	-0.3031522369169437
2201	90-91	-0.2564834612072495
2201	92-93	-0.25332085368095747
2201	94-95	-0.22698040453142454
2201	96-97	-0.20886599905303171
2201	98-99	-0.25722352840675455
2201	100-101	-0.2585082925747493
2202	1	-1.6632066716905207
2202	2	-2.1600796588948725
2202	3	-1.5459163129273215
2202	4	-1.8353573245416221
2202	5	-1.953662077596217
2202	6	-0.8558989904593091
2202	7	-0.4323805694535281
2202	8	-0.22130653281803347
2202	9	-0.15447279592778784
2202	10-11	-0.1113965031102282
2202	12-13	-0.16963354524529706
2202	14-15	-0.1880480412857679
2202	16-17	-0.2791332497797896
2202	18-19	-0.31688390957852874
2202	20-21	-0.4218989347882527
2202	22-23	-0.2844993898412298
2202	24-25	-0.2925239406838571
2202	26-27	-0.35169332720451507
2202	28-29	-0.36580667590269655
2202	30-31	-0.3436655211516779
2202	32-33	-0.425031763128068
2202	34-35	-0.3908989316353484
2202	36-37	-0.4397477461603003
2202	38-39	-0.4409230944542699
2202	40-41	-0.3811037478105703
2202	42-43	-0.43503334745037847
2202	44-45	-0.47274587892918873
2202	46-47	-0.5133711609626133
2202	48-49	-0.45090464867353575
2202	50-51	-0.4322557406632015
2202	52-53	-0.43043833630312633
2202	54-55	-0.4294426673556657
2202	56-57	-0.40128937025060196
2202	58-59	-0.4969129766031841
2202	60-61	-0.47067028611121486
2202	62-63	-0.45809457220632055
2202	64-65	-0.5069365690386505
2202	66-67	-0.4650528389254234
2202	68-69	-0.4789021703933187
2202	70-71	-0.5066487150154941
2202	72-73	-0.525910792962911
2202	74-75	-0.4941223608239298
2202	76-77	-0.5627998171380852
2202	78-79	-0.5241082109031083
2202	80-81	-0.5106925771938577
2202	82-83	-0.49369165704053586
2202	84-85	-0.45674038670310324
2202	86-87	-0.47102146479932827
2202	88-89	-0.5037948299109516
2202	90-91	-0.5093183324743507
2202	92-93	-0.4698408547632944
2202	94-95	-0.48771518477262177
2202	96-97	-0.4702750480615343
2202	98-99	-0.45370597137560154
2202	100-101	-0.4495871765493682
2203	1	-3.7656190993719783
2203	2	-4.118328936442868
2203	3	-2.728082473130428
2203	4	-2.0601529489469677
2203	5	-2.1673494352281892
2203	6	-0.9776883982940987
2203	7	-0.4910257924336605
2203	8	-0.3102119222395032
2203	9	-0.23865353131127165
2203	10-11	-0.2380852529940114
2203	12-13	-0.1752511528116898
2203	14-15	-0.21785991651196923
2203	16-17	-0.2347682039780068
2203	18-19	-0.3072999321107872
2203	20-21	-0.44292453984164837
2203	22-23	-0.4522952385037371
2203	24-25	-0.404767999498965
2203	26-27	-0.45885261867373117
2203	28-29	-0.5462469751008356
2203	30-31	-0.6253884914350607
2203	32-33	-0.6996501968608584
2203	34-35	-0.7503690482636429
2203	36-37	-0.7048425274955648
2203	38-39	-0.7395371450386037
2203	40-41	-0.7503363263275062
2203	42-43	-0.7467413045103726
2203	44-45	-0.7647066705615089
2203	46-47	-0.7106023990016581
2203	48-49	-0.8218637984239692
2203	50-51	-0.8465043134066903
2203	52-53	-0.846978142661257
2203	54-55	-0.7245578541650204
2203	56-57	-0.7217872864719155
2203	58-59	-0.8537893108577919
2203	60-61	-0.8628903526969935
2203	62-63	-0.8273401390066653
2203	64-65	-0.8383818431349397
2203	66-67	-0.8062137454296376
2203	68-69	-0.8117226789491809
2203	70-71	-0.8111098584822614
2203	72-73	-0.8427551527430666
2203	74-75	-0.8164908454494082
2203	76-77	-1.0454588827449989
2203	78-79	-0.9226322760874233
2203	80-81	-0.8764493417207362
2203	82-83	-0.839769288589423
2203	84-85	-0.8254602276271399
2203	86-87	-0.8285962638324484
2203	88-89	-0.8313294645907803
2203	90-91	-0.8490100163630423
2203	92-93	-0.8212863064777061
2203	94-95	-0.8804749558738578
2203	96-97	-0.8852650466473868
2203	98-99	-0.9590895937100363
2203	100-101	-0.8953488428534335
2204	1	-1.2341966227345509
2204	2	-0.811316577315548
2204	3	-1.112877674629825
2204	4	-1.166019312366089
2204	5	-1.625553190160943
2204	6	-0.6910136567568941
2204	7	-0.379597423042739
2204	8	-0.2275237664840546
2204	9	-0.19008483919461128
2204	10-11	-0.186815394049205
2204	12-13	-0.2744034887214255
2204	14-15	-0.2471496511766702
2204	16-17	-0.28684081254139215
2204	18-19	-0.38810292073339525
2204	20-21	-0.4512407709010873
2204	22-23	-0.43624285481907776
2204	24-25	-0.45195872510399937
2204	26-27	-0.48197097610320583
2204	28-29	-0.5355462579102195
2204	30-31	-0.49319775641727404
2204	32-33	-0.6138694376232934
2204	34-35	-0.5882199763148108
2204	36-37	-0.5351267681646519
2204	38-39	-0.6297680190117347
2204	40-41	-0.6520182141125588
2204	42-43	-0.6737065020430109
2204	44-45	-0.657444739575034
2204	46-47	-0.6493716131320255
2204	48-49	-0.6737808721007141
2204	50-51	-0.6591288306382879
2204	52-53	-0.680557978133713
2204	54-55	-0.6353645409857549
2204	56-57	-0.6155880018973576
2204	58-59	-0.705154178617704
2204	60-61	-0.6978544779377813
2204	62-63	-0.6495630974113809
2204	64-65	-0.6148598269704095
2204	66-67	-0.5994054595573246
2204	68-69	-0.6821057998690137
2204	70-71	-0.6334918922461767
2204	72-73	-0.5780294143483147
2204	74-75	-0.6105428123791441
2204	76-77	-0.7594255200767321
2204	78-79	-0.5982170353745673
2204	80-81	-0.5724921752194589
2204	82-83	-0.5798221301374902
2204	84-85	-0.5480819654686684
2204	86-87	-0.5408171679108449
2204	88-89	-0.5505325072089882
2204	90-91	-0.549247815818422
2204	92-93	-0.5212242492481423
2204	94-95	-0.4954307322285665
2204	96-97	-0.5106423778882636
2204	98-99	-0.5585233570484078
2204	100-101	-0.5846403392936956
2205	1	-3.6611590693017746
2205	2	-3.676215553457155
2205	3	-1.3821153318244939
2205	4	-1.6450832195443539
2205	5	-3.80452308795428
2205	6	-1.702473260315557
2205	7	-0.8365881440859226
2205	8	-0.4734087978441579
2205	9	-0.39531989939018075
2205	10-11	-0.3142353031653329
2205	12-13	-0.32850776041545515
2205	14-15	-0.3912871747905484
2205	16-17	-0.38402465726991863
2205	18-19	-0.45892129239644674
2205	20-21	-0.568621612109844
2205	22-23	-0.6642075621543455
2205	24-25	-0.7203177202200308
2205	26-27	-0.7130511376210364
2205	28-29	-0.8674202093769665
2205	30-31	-0.7545666266843369
2205	32-33	-0.9287930300529297
2205	34-35	-1.123509529940769
2205	36-37	-1.1114582471072154
2205	38-39	-1.1082933521162985
2205	40-41	-1.1054320610912782
2205	42-43	-1.1462435053097337
2205	44-45	-1.2050753142998332
2205	46-47	-1.22275621247406
2205	48-49	-1.2216849568416919
2205	50-51	-1.1835883336336863
2205	52-53	-1.2692151957193758
2205	54-55	-1.2219523389968714
2205	56-57	-1.244405106632783
2205	58-59	-1.318999252085149
2205	60-61	-1.3570072496358634
2205	62-63	-1.4046731202415188
2205	64-65	-1.3462879160799517
2205	66-67	-1.3841338296050196
2205	68-69	-1.3371573053671213
2205	70-71	-1.3867990348084689
2205	72-73	-1.3216635555641432
2205	74-75	-1.3625210383413418
2205	76-77	-1.346466946523055
2205	78-79	-1.3239237684218423
2205	80-81	-1.3480562458895022
2205	82-83	-1.4947303410527297
2205	84-85	-1.416215928305519
2205	86-87	-1.353604378381256
2205	88-89	-1.309739821993638
2205	90-91	-1.2886132172529976
2205	92-93	-1.3100968149210992
2205	94-95	-1.4071874011067607
2205	96-97	-1.4701420449143612
2205	98-99	-1.5060863906806077
2205	100-101	-1.591061927542878
2206	1	-1.7861555022248972
2206	2	-2.708846012209296
2206	3	-3.595834633425344
2206	4	-2.594968453073399
2206	5	-2.3256742503405228
2206	6	-1.9499592080960966
2206	7	-0.9418504900326425
2206	8	-0.5990785168639476
2206	9	-0.5782057380146313
2206	10-11	-0.46912307795735586
2206	12-13	-0.6427145694012424
2206	14-15	-0.6853328622042554
2206	16-17	-0.5021678575058957
2206	18-19	-0.638416718035181
2206	20-21	-0.8963827411479528
2206	22-23	-0.944602471984112
2206	24-25	-0.8770142128141316
2206	26-27	-1.0042204110388582
2206	28-29	-1.103535228074385
2206	30-31	-1.1517631047415051
2206	32-33	-1.3641483630823075
2206	34-35	-1.48026637298247
2206	36-37	-1.3757314807053902
2206	38-39	-1.4450764820543398
2206	40-41	-1.4552032212355144
2206	42-43	-1.5266458516675883
2206	44-45	-1.5180175300646042
2206	46-47	-1.52496556728196
2206	48-49	-1.5565806279075147
2206	50-51	-1.585146378053686
2206	52-53	-1.6247387858176836
2206	54-55	-1.624097817305021
2206	56-57	-1.572147000134045
2206	58-59	-1.7051794134938731
2206	60-61	-1.6110860181071018
2206	62-63	-1.8003337016838188
2206	64-65	-1.6503234320804623
2206	66-67	-1.6176047894698868
2206	68-69	-1.5794341253873014
2206	70-71	-1.5639195197694633
2206	72-73	-1.6025163270306209
2206	74-75	-1.683762622494065
2206	76-77	-1.4782806347778426
2206	78-79	-1.6984038057653947
2206	80-81	-1.6143702888446931
2206	82-83	-1.5892869673890466
2206	84-85	-1.6450645021277452
2206	86-87	-1.6771615597627942
2206	88-89	-1.6964548362086127
2206	90-91	-1.7555456700962289
2206	92-93	-1.7415742622554866
2206	94-95	-1.7353293662416078
2206	96-97	-1.895196566063614
2206	98-99	-1.9528139754747436
2206	100-101	-1.8150967048050042
2207	1	2.6585375238344966
2207	2	2.5259332640647756
2207	3	1.7526759956793576
2207	4	1.25633497847344
2207	5	2.086788393137759
2207	6	1.0868810082953289
2207	7	0.6118081176216563
2207	8	0.3959464957661396
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2210	52-53	0.20317526378146056
2210	54-55	0.2599065595250565
2210	56-57	0.3196794996332457
2210	58-59	0.250574937656765
2210	60-61	0.25737526049243087
2210	62-63	0.21325762656395142
2210	64-65	0.21274538077336302
2210	66-67	0.20735111793345595
2210	68-69	0.23512619209847685
2210	70-71	0.201969199498798
2210	72-73	0.1394249602826676
2210	74-75	0.2401354569746772
2210	76-77	0.33457860632350034
2210	78-79	0.25079674089577964
2210	80-81	0.2533110171051334
2210	82-83	0.2618184410637383
2210	84-85	0.2686817332367468
2210	86-87	0.2673381003810036
2210	88-89	0.273175316260847
2210	90-91	0.3244753501715074
2210	92-93	0.3135560947929683
2210	94-95	0.2715608838560435
2210	96-97	0.3072216442670914
2210	98-99	0.33440174735297035
2210	100-101	0.37948540025674404
2209	1	-0.13402402463180962
2209	2	0.6767000627746071
2209	3	0.8286585619533255
2209	4	0.12603937976182777
2209	5	0.1822474246394279
2209	6	0.22101639702058407
2209	7	0.11583541789145357
2209	8	0.013916688545471345
2209	9	0.11947074548593406
2209	10-11	0.07542506405314242
2209	12-13	0.060882561835569504
2209	14-15	0.05090725019040576
2209	16-17	0.0020829200429233197
2209	18-19	-0.03706181127346042
2209	20-21	-0.010769211324593186
2209	22-23	0.01510424499630858
2209	24-25	0.007523534732804649
2209	26-27	-0.002079672463437987
2209	28-29	0.06702221566640532
2209	30-31	0.08374918251369934
2209	32-33	0.08108281076766843
2209	34-35	0.10021719281726149
2209	36-37	0.06762695417966569
2209	38-39	0.0998905100459595
2209	40-41	0.1165883422159979
2209	42-43	0.110020934254635
2209	44-45	0.13846458456310273
2209	46-47	0.08646395141821728
2209	48-49	0.14499805592677006
2209	50-51	0.11696331374788116
2209	52-53	0.12176023533014302
2209	54-55	0.1949033030983358
2209	56-57	0.22876462434398093
2209	58-59	0.1716012647339582
2209	60-61	0.16099506649555906
2209	62-63	0.17899364368894766
2209	64-65	0.11594138666930576
2209	66-67	0.09302815693939692
2209	68-69	0.1367965790588812
2209	70-71	0.1448561933479695
2209	72-73	0.15658616418005522
2209	74-75	0.15708558433728825
2209	76-77	0.1924137194958675
2209	78-79	0.1437316020181001
2209	80-81	0.19015351276404857
2209	82-83	0.19443208280267044
2209	84-85	0.1826160867500164
2209	86-87	0.18688546808221673
2209	88-89	0.17263796848281743
2209	90-91	0.19166165384276468
2209	92-93	0.19624108680784857
2209	94-95	0.19903713052255512
2209	96-97	0.17222280970907988
2209	98-99	0.17178376749278712
2209	100-101	0.2048866129892737
2208	1	-1.3915002082577068
2208	2	-0.5772819200473336
2208	3	-0.6447594357929063
2208	4	-0.6344155682745907
2208	5	-0.17699244000483105
2208	6	-0.04472343347954677
2208	7	-0.010190613979283114
2208	8	-0.02069557678374423
2208	9	0.01804346770541798
2208	10-11	0.0331011641364114
2208	12-13	0.003037654298509551
2208	14-15	-0.061416734209828405
2208	16-17	0.007338605566701517
2208	18-19	-0.027535219185487847
2208	20-21	-0.03863474899797126
2208	22-23	-0.03553925882664544
2208	24-25	-0.02032375043808088
2208	26-27	-0.06998501527802148
2208	28-29	-0.08162065841298016
2208	30-31	-0.05619553388093834
2208	32-33	-0.07773236116004512
2208	34-35	-0.10789211785836983
2208	36-37	-0.08600920376452592
2208	38-39	-0.08080583058283963
2208	40-41	-0.052435602821645944
2208	42-43	-0.07820134337476503
2208	44-45	-0.1125971880944121
2208	46-47	-0.056445638312986546
2208	48-49	-0.09306002459202745
2208	50-51	-0.056104955491804276
2208	52-53	-0.1023680733972725
2208	54-55	0.005998861673326417
2208	56-57	0.01772386869701137
2208	58-59	0.003234355566206659
2208	60-61	-0.09155552745720286
2208	62-63	-0.07688131629107176
2208	64-65	-0.09452695269082767
2208	66-67	-0.08896536281744005
2208	68-69	-0.03545498277496506
2208	70-71	-0.013354110500991112
2208	72-73	-0.08512797785118664
2208	74-75	-0.05312602238443276
2208	76-77	-0.14077934107966072
2208	78-79	-0.052153794147479005
2208	80-81	-0.035733558918792596
2208	82-83	0.018912819679393067
2208	84-85	-0.01643898932608323
2208	86-87	-0.013975289658823442
2208	88-89	-0.01664601928109022
2208	90-91	-0.01176586100711674
2208	92-93	-0.04175431536347318
2208	94-95	0.008367466568103055
2208	96-97	-0.018996837044170434
2208	98-99	0.04796966875472464
2208	100-101	0.05065265231324645
2213	1	-1.890241545580949
2213	2	-1.278609614634334
2213	3	-1.4312235225356424
2213	4	-1.1707800748180617
2213	5	-1.8314636148023489
2213	6	-1.2478107688010525
2213	7	-0.5706820140049587
2213	8	-0.27538697649001875
2213	9	-0.15581156458373613
2213	10-11	-0.06673699005344247
2213	12-13	-0.03536933290422439
2213	14-15	-0.06610144433452092
2213	16-17	-0.07282495292871971
2213	18-19	-0.09394271075282035
2213	20-21	-0.19426431163784486
2213	22-23	-0.18124189024543114
2213	24-25	-0.2334594233881191
2213	26-27	-0.17831684217686927
2213	28-29	-0.14665254696682695
2213	30-31	-0.20771080604748704
2213	32-33	-0.19934655505768717
2213	34-35	-0.2260972990473462
2213	36-37	-0.19264302509704834
2213	38-39	-0.28169911596411623
2213	40-41	-0.2560471340278312
2213	42-43	-0.21137615846638624
2213	44-45	-0.22554735275738125
2213	46-47	-0.23439171637120637
2213	48-49	-0.28274393356391414
2213	50-51	-0.26407259552546947
2213	52-53	-0.23395505435047426
2213	54-55	-0.15273606313954957
2213	56-57	-0.19119370966651417
2213	58-59	-0.25586241569769186
2213	60-61	-0.2904186892215108
2213	62-63	-0.24312436851761987
2213	64-65	-0.22917244056387176
2213	66-67	-0.2568704391890506
2213	68-69	-0.2849418911364552
2213	70-71	-0.2328084836351607
2213	72-73	-0.1995291838943416
2213	74-75	-0.1865825707338118
2213	76-77	-0.4071209738258439
2213	78-79	-0.21783934818861184
2213	80-81	-0.22488091992164527
2213	82-83	-0.16024447580616652
2213	84-85	-0.242082739953716
2213	86-87	-0.2399114644537832
2213	88-89	-0.21607314022472934
2213	90-91	-0.24350338807662197
2213	92-93	-0.23478270277881919
2213	94-95	-0.22255343836183528
2213	96-97	-0.21962284107506136
2213	98-99	-0.20406503235640727
2213	100-101	-0.1975753726121603
2212	1	-1.9149063464894915
2212	2	-2.8722958775555796
2212	3	-0.7963949752774973
2212	4	-1.1253616119246317
2212	5	-2.218627256854912
2212	6	-0.9723000030290265
2212	7	-0.4106657320784777
2212	8	-0.2030845034913682
2212	9	-0.22719709279029132
2212	10-11	-0.09708597953103038
2212	12-13	-0.12848848068153984
2212	14-15	-0.16247842597825013
2212	16-17	-0.16461648632694903
2212	18-19	-0.12224124943542591
2212	20-21	-0.17947079080160933
2212	22-23	-0.23955117731723163
2212	24-25	-0.23504995321707867
2212	26-27	-0.2605144966523625
2212	28-29	-0.38552028673413474
2212	30-31	-0.33527767203738534
2212	32-33	-0.3566243154526205
2212	34-35	-0.35391404335735643
2212	36-37	-0.39875207923879685
2212	38-39	-0.3672704899037669
2212	40-41	-0.38447952057583734
2212	42-43	-0.3603985185924756
2212	44-45	-0.4212346702375669
2212	46-47	-0.39287468698262273
2212	48-49	-0.3980204076287137
2212	50-51	-0.4660590195276981
2212	52-53	-0.4785178469616085
2212	54-55	-0.37330763987898763
2212	56-57	-0.48414887932302975
2212	58-59	-0.4536820281762459
2212	60-61	-0.5036662961839085
2212	62-63	-0.4666937608135484
2212	64-65	-0.4362230651872423
2212	66-67	-0.43759330457633894
2212	68-69	-0.42327235630124704
2212	70-71	-0.4275897205980428
2212	72-73	-0.37911854327875716
2212	74-75	-0.43513979504196953
2212	76-77	-0.5121466424857424
2212	78-79	-0.4701672001331687
2212	80-81	-0.4426169209414397
2212	82-83	-0.4644926969622425
2212	84-85	-0.39003460687069946
2212	86-87	-0.4217830458286471
2212	88-89	-0.41194080466638994
2212	90-91	-0.4390335357148736
2212	92-93	-0.4112366324594383
2212	94-95	-0.4355004670551139
2212	96-97	-0.4734506872253377
2212	98-99	-0.45772846581489546
2212	100-101	-0.4105419570000528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2176.0
3	103.0
4	161.0
5	360.0
6	709.0
7	1796.0
8	4022.0
9	7144.0
10	10182.0
11	13969.0
12	20746.0
13	34738.0
14	39048.0
15	43641.0
16	47804.0
17	47929.0
18	47059.0
19	48770.0
20	50786.0
21	53364.0
22	56719.0
23	63660.0
24	76084.0
25	95614.0
26	123400.0
27	157651.0
28	198527.0
29	252431.0
30	321893.0
31	412345.0
32	528627.0
33	689226.0
34	937689.0
35	1346116.0
36	2062906.0
37	3614164.0
38	4220298.0
39	368006.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.237641047705992	42.0324570198809	13.5414673099085	36.18843462250461
2	8.336527177551737	45.63362744200954	20.74961836374173	25.280227016696994
3	5.568926074257306	18.054112756899844	44.57540865255859	31.80155251628426
4	8.584568631841991	40.12063594406624	12.406213138001448	38.888582286090326
5	9.643600379975261	42.75461064379481	15.311110526388486	32.29067844984144
6	8.2577875	47.44805	18.18198125	26.11218125
7	6.0933375	29.951506249999998	39.1619875	24.79316875
8	6.34151875	32.77189375	16.14129375	44.745293749999995
9	6.02318125	56.822931249999996	17.7279375	19.42595
10-11	5.96409375	45.41955625	28.667074999999997	19.949275
12-13	5.035621875	57.674534375	16.329009375000002	20.960834375
14-15	5.7066875	45.886290625	15.881006249999999	32.526015625
16-17	5.576123129484715	55.90717219804701	16.502465832825493	22.01423883964279
18-19	18.210390625	30.728499999999997	17.718684375	33.342425
20-21	28.68448132419087	31.678952039312275	17.705570994280965	21.93099564221589
22-23	3.5916812499999997	45.5205375	17.205828125	33.681953125
24-25	3.437607501505021	44.854946719254066	16.794360121041695	34.913085658199215
26-27	3.4330415459583943	45.00128766948885	29.23930686662372	22.326363917929033
28-29	3.489539696651984	43.7797594028666	16.710194362108215	36.0205065383732
30-31	3.4508697655145664	32.903083923058325	29.342001387769596	34.304044923657514
32-33	3.5050304699456336	45.56418048022849	21.267711738911128	29.66307731091476
34-35	3.5398665377371255	45.00479954519868	20.924876437922148	30.530457479142047
36-37	3.4902435513546823	44.8205327349539	21.13851162554058	30.55071208815083
38-39	3.495699933737442	44.981889359153186	21.390493716682002	30.131916990427364
40-41	3.5026327824018426	44.81064147965565	21.223806160415197	30.462919577527316
42-43	3.484782456179277	44.448378196913914	21.386094089532573	30.680745257374237
44-45	3.5081089353054242	44.76577101830825	21.345432228372125	30.380687818014195
46-47	3.510861031879748	44.72399433321706	21.172888692415526	30.592255942487668
48-49	3.512633486666318	44.4845032309428	21.345188342443127	30.65767493994776
50-51	3.469486786407505	44.60295284566992	21.450628279397957	30.47693208852462
52-53	3.524500799001598	44.56501100502201	21.3245613991228	30.585926796853595
54-55	3.4924673822397807	44.306784977877115	21.489884124005645	30.710863515877456
56-57	3.4653187500000002	44.5621625	21.484803125	30.487715625
58-59	3.520471875	44.532725	21.32996875	30.616834375000003
60-61	3.4607646114186332	44.34217412864241	21.4638381271704	30.733223132768554
62-63	3.486950634702398	44.50054694495213	21.561746903801566	30.450755516543914
64-65	3.47690195575735	44.53400317537678	21.33116824878214	30.657926620083725
66-67	3.449978125	44.318065625	21.479540625	30.752415625
68-69	3.4562320276815814	44.52585266969222	21.537219515582326	30.480695787043878
70-71	3.4771125	44.5389	21.319771874999997	30.664215625
72-73	3.4568801898798975	44.2693231634699	21.51640904282136	30.75738760382885
74-75	3.464674394957062	44.44212499412889	21.59357340873269	30.499627202181358
76-77	3.4834163175029276	44.45514128142767	21.436379020331056	30.62506338073835
78-79	3.472739391560211	44.25048721427187	21.55916942056572	30.71760397360219
80-81	3.4870284519089174	44.47060416911914	21.62167702703222	30.420690351939722
82-83	3.5157790413890124	44.46770811624228	21.435841781739967	30.580671060628745
84-85	3.5241814970346246	44.24069360861942	21.55790497516413	30.67721991918183
86-87	3.563346875	44.4416125	21.644084375	30.35095625
88-89	3.6039520593072027	44.458098425603225	21.438916417166315	30.499033097923256
90-91	3.64378543902104	44.24658614748583	21.555640598997506	30.553987814495624
92-93	3.7042528731788127	44.44825899599246	21.560836146504325	30.286651984324404
94-95	3.7901767520362895	44.43204787998278	21.389403028075026	30.3883723399059
96-97	3.875341944509862	44.21070944454361	21.49504064833329	30.418907962613233
98-99	4.00125625	44.373656249999996	21.537243750000002	30.087843749999998
100-101	4.19001875	44.35325	21.270678125	30.186053125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	4.5
2	6.5
3	7.0
4	10.0
5	17.0
6	22.5
7	50.0
8	78.5
9	107.0
10	171.5
11	253.0
12	338.5
13	506.0
14	785.5
15	1166.0
16	1685.0
17	2543.5
18	3903.5
19	6445.5
20	11403.5
21	21016.5
22	39367.5
23	71978.5
24	125547.0
25	206427.0
26	318242.5
27	462985.0
28	633186.0
29	814077.5
30	986464.0
31	1126298.0
32	1217003.0
33	1248154.5
34	1218609.5
35	1137583.5
36	1019087.0
37	881165.0
38	740212.5
39	605790.0
40	485858.5
41	386227.5
42	305721.0
43	242914.0
44	196448.0
45	162783.0
46	136589.5
47	116775.0
48	100539.5
49	84825.5
50	72927.0
51	65112.0
52	59505.0
53	56415.0
54	55003.5
55	52853.5
56	50684.0
57	49233.0
58	47895.5
59	45943.5
60	43491.0
61	40707.0
62	37337.5
63	33735.0
64	30307.5
65	26915.0
66	23259.0
67	19427.0
68	16002.5
69	13026.0
70	10147.5
71	7722.0
72	5854.0
73	4311.0
74	3057.5
75	2108.0
76	1437.0
77	1003.0
78	705.0
79	484.5
80	341.0
81	262.5
82	204.5
83	159.0
84	130.0
85	112.0
86	104.5
87	99.0
88	97.0
89	82.0
90	71.5
91	66.5
92	66.5
93	62.0
94	43.5
95	27.5
96	25.5
97	22.0
98	14.5
99	10.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0039125
2	0.00295
3	2.4375E-4
4	0.00181875
5	6.875E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.9062499999999998E-4
18-19	0.0
20-21	6.5625E-5
22-23	0.0
24-25	0.0014
26-27	0.001028125
28-29	0.00140625
30-31	0.005915625
32-33	3.34375E-4
34-35	2.90625E-4
36-37	0.00491875
38-39	8.75E-5
40-41	4.375E-5
42-43	0.001828125
44-45	1.65625E-4
46-47	5.8125E-4
48-49	0.00113125
50-51	7.0E-4
52-53	1.9999999999999998E-4
54-55	3.1875E-4
56-57	0.0
58-59	0.0
60-61	5.125E-4
62-63	0.0013625
64-65	5.625E-5
66-67	0.0
68-69	2.9374999999999996E-4
70-71	0.0
72-73	0.0052125
74-75	2.53125E-4
76-77	0.002890625
78-79	6.843750000000001E-4
80-81	9.375000000000001E-6
82-83	5.59375E-4
84-85	0.001603125
86-87	0.0
88-89	0.001184375
90-91	0.0020875
92-93	0.017371875
94-95	0.003096875
96-97	0.00205
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.75420118004112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.48568723506511	87.61212956380649
2	3.260804910677554	5.983850995663486
3	0.5765147878132257	1.5869296147285026
4	0.28605922503304054	1.0498854273235303
5	0.14715298869869567	0.6750952464652219
6	0.07722922164464999	0.42516633238567203
7	0.03894884623531495	0.25016041912439024
8	0.022157698486159824	0.16264495396686407
9	0.012123482584562592	0.10011424140600128
>10	0.09008924374085667	1.7720373445035322
>50	0.002624117692512877	0.1522564152134802
>100	4.972406820658495E-4	0.09304014538813177
>500	8.228664479697125E-5	0.043099490016554914
>1k	2.180347321080341E-5	0.03970508562155539
>5k	6.91152827446127E-6	0.053884724386626566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.00400625	0.0	0.0
2	0.0041375	0.0	0.0
3	0.0042125	0.0	0.0
4	0.004275	0.0	0.0
5	0.004325	0.0	0.0
6	0.004475	0.0	0.0
7	0.0046125	0.0	0.0
8	0.00466875	0.0	1.25E-5
9	0.0047375	0.0	1.25E-5
10-11	0.00488125	6.25E-6	1.25E-5
12-13	0.005203124999999999	9.375000000000001E-6	1.25E-5
14-15	0.0056	1.25E-5	1.25E-5
16-17	0.006009375000000001	1.25E-5	1.25E-5
18-19	0.006434375	1.25E-5	1.25E-5
20-21	0.006953125	1.25E-5	1.25E-5
22-23	0.010234375	1.25E-5	1.5625E-5
24-25	0.016978125	1.5625E-5	1.875E-5
26-27	0.019059375	2.5E-5	1.875E-5
28-29	0.01990625	2.5E-5	1.875E-5
30-31	0.020734375	2.5E-5	1.875E-5
32-33	0.021665625	2.5E-5	1.875E-5
34-35	0.022359375	2.5E-5	1.875E-5
36-37	0.023109375	2.5E-5	1.875E-5
38-39	0.02403125	3.125E-5	1.875E-5
40-41	0.025031249999999998	3.125E-5	1.875E-5
42-43	0.026234374999999997	3.125E-5	1.875E-5
44-45	0.0275375	3.125E-5	2.5E-5
46-47	0.029178125	3.125E-5	2.5E-5
48-49	0.031225	3.125E-5	2.5E-5
50-51	0.033528125	3.125E-5	2.5E-5
52-53	0.036009374999999996	3.125E-5	2.5E-5
54-55	0.03940625	3.75E-5	2.5E-5
56-57	0.043524999999999994	3.75E-5	2.5E-5
58-59	0.048428125	3.75E-5	2.5E-5
60-61	0.05425	3.75E-5	2.5E-5
62-63	0.061196875	3.75E-5	2.5E-5
64-65	0.07023125	4.375E-5	2.5E-5
66-67	0.08139687500000001	4.375E-5	2.5E-5
68-69	0.09535625	4.375E-5	2.5E-5
70-71	0.113146875	4.375E-5	2.5E-5
72-73	0.13494375	4.375E-5	2.5E-5
74-75	0.1629375	4.375E-5	2.5E-5
76-77	0.198490625	4.375E-5	2.5E-5
78-79	0.244153125	4.375E-5	2.5E-5
80-81	0.30173125	5.0E-5	2.5E-5
82-83	0.374840625	5.0E-5	2.5E-5
84-85	0.46840625	5.0E-5	3.125E-5
86-87	0.586265625	5.625E-5	3.125E-5
88-89	0.734478125	5.625E-5	3.125E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAATC	451845	0.0	79.468124	2
AATAAAT	459520	0.0	76.54337	1
TAAATCA	478420	0.0	76.04054	3
TCATAAA	524150	0.0	71.77938	7
AATCATA	517960	0.0	71.777054	5
AAATCAT	520330	0.0	70.188576	4
ATCATAA	541480	0.0	69.88997	6
CATAAAC	570685	0.0	65.843925	8
ATAAACA	640390	0.0	58.67251	9
AGAAATC	5590	0.0	53.786068	2
GATAAAT	6415	0.0	49.832336	1
GTAAATC	2850	0.0	48.49824	2
GTGCAAC	1890	0.0	48.000908	2
GTCCCCA	1820	0.0	47.238926	1
AGTAAAT	2805	0.0	46.737885	1
CGTGCAA	1895	0.0	45.61997	1
CGCTTGT	1955	0.0	43.24584	3
AGTCATA	1610	0.0	43.071632	5
AAGAAAT	6900	0.0	41.648476	1
ATGAATC	1895	0.0	41.607994	2
>>END_MODULE