##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	2212_lane2_GCCAAT_L002_R1_006.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255678
Sequences flagged as poor quality	0
Sequence length	101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.49786841261274	30.0	16.0	31.0	16.0	33.0
2	25.37767426215787	28.0	16.0	31.0	16.0	34.0
3	28.72584657264215	31.0	28.0	31.0	16.0	34.0
4	32.31198225893507	35.0	32.0	37.0	22.0	37.0
5	29.616932235076934	33.0	26.0	35.0	16.0	37.0
6	32.731803283817925	35.0	32.0	37.0	28.0	37.0
7	34.123862827462666	35.0	33.0	37.0	30.0	37.0
8	34.69393534054553	35.0	35.0	37.0	32.0	37.0
9	36.41524495654691	38.0	35.0	39.0	32.0	39.0
10-11	36.583458490757906	38.5	35.0	39.0	32.0	39.0
12-13	36.49808157135146	38.0	35.0	39.0	32.0	39.0
14-15	37.74514232745875	40.0	36.5	41.0	32.5	41.0
16-17	37.74309287463137	40.0	37.0	41.0	32.5	41.0
18-19	37.65985536495123	40.0	36.5	40.5	32.5	41.0
20-21	37.77313065652892	40.0	37.0	41.0	32.0	41.0
22-23	37.60587927001932	39.5	37.0	40.5	32.0	41.0
24-25	37.502198077269064	39.5	36.5	40.0	32.0	41.0
26-27	37.2207033847261	39.5	36.0	40.0	31.5	41.0
28-29	37.06129780426943	39.0	36.0	40.0	30.5	41.0
30-31	37.14604698096825	39.0	36.0	40.0	30.5	41.0
32-33	36.800858892826135	39.0	36.0	40.0	30.0	41.0
34-35	36.54262196982142	39.0	35.5	40.0	30.0	41.0
36-37	36.44302599363262	39.0	35.0	40.0	30.0	41.0
38-39	36.24083417423478	39.0	35.0	40.0	30.0	41.0
40-41	36.27381706677931	39.0	35.0	40.0	30.0	41.0
42-43	36.20462065566846	39.0	35.0	40.0	29.5	41.0
44-45	35.95423931664047	39.0	35.0	40.0	28.0	41.0
46-47	35.799994524362674	39.0	35.0	40.0	27.5	41.0
48-49	35.757937718536596	39.0	35.0	40.0	28.0	41.0
50-51	35.60408990996488	39.0	34.5	40.0	27.5	41.0
52-53	35.41190090660909	38.5	34.0	40.0	26.5	41.0
54-55	35.326672611644334	38.0	34.0	40.0	26.5	41.0
56-57	35.108382418510786	38.0	34.0	40.0	26.0	41.0
58-59	34.92909440780983	38.0	34.0	40.0	26.0	41.0
60-61	34.47910848802009	37.5	33.0	40.0	25.5	41.0
62-63	34.41078231212697	37.0	33.0	40.0	26.0	41.0
64-65	34.16595092264489	37.0	33.0	39.5	26.0	41.0
66-67	33.598141021128136	36.0	32.5	39.0	25.5	40.0
68-69	33.22629244596719	36.0	32.0	39.0	25.0	40.0
70-71	32.864174860566806	35.0	32.0	37.5	25.0	39.5
72-73	32.4187708758673	35.0	32.0	37.0	24.5	39.0
74-75	31.96122466539945	35.0	32.0	36.5	23.0	39.0
76-77	30.02830708938587	33.0	29.5	35.0	20.0	36.5
78-79	30.97974014189723	34.0	31.0	35.0	21.0	37.0
80-81	30.842990010873052	34.0	31.0	35.0	20.5	36.0
82-83	30.767404704354696	34.0	31.0	35.0	20.5	36.0
84-85	30.708201331362105	34.0	31.0	35.0	20.5	36.0
86-87	30.35801867974562	34.0	31.0	35.0	20.0	35.5
88-89	30.25783993929865	34.0	31.0	35.0	20.0	35.0
90-91	29.972719592612584	34.0	30.5	35.0	18.0	35.0
92-93	29.820983815580536	34.0	30.5	35.0	15.0	35.0
94-95	29.656255133409992	34.0	30.0	35.0	10.5	35.0
96-97	29.53494434405776	34.0	30.5	35.0	4.5	35.0
98-99	29.283862905686057	34.0	30.0	35.0	2.0	35.0
100-101	28.598170354899523	33.5	29.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2316	1	0.0
2316	2	0.0
2316	3	0.0
2316	4	0.0
2316	5	0.0
2316	6	0.0
2316	7	0.0
2316	8	0.0
2316	9	0.0
2316	10-11	0.0
2316	12-13	0.0
2316	14-15	0.0
2316	16-17	0.0
2316	18-19	0.0
2316	20-21	0.0
2316	22-23	0.0
2316	24-25	0.0
2316	26-27	0.0
2316	28-29	0.0
2316	30-31	0.0
2316	32-33	0.0
2316	34-35	0.0
2316	36-37	0.0
2316	38-39	0.0
2316	40-41	0.0
2316	42-43	0.0
2316	44-45	0.0
2316	46-47	0.0
2316	48-49	0.0
2316	50-51	0.0
2316	52-53	0.0
2316	54-55	0.0
2316	56-57	0.0
2316	58-59	0.0
2316	60-61	0.0
2316	62-63	0.0
2316	64-65	0.0
2316	66-67	0.0
2316	68-69	0.0
2316	70-71	0.0
2316	72-73	0.0
2316	74-75	0.0
2316	76-77	0.0
2316	78-79	0.0
2316	80-81	0.0
2316	82-83	0.0
2316	84-85	0.0
2316	86-87	0.0
2316	88-89	0.0
2316	90-91	0.0
2316	92-93	0.0
2316	94-95	0.0
2316	96-97	0.0
2316	98-99	0.0
2316	100-101	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	1.0
4	5.0
5	13.0
6	18.0
7	66.0
8	106.0
9	183.0
10	251.0
11	443.0
12	580.0
13	947.0
14	1065.0
15	1237.0
16	1330.0
17	1315.0
18	1229.0
19	1233.0
20	1329.0
21	1358.0
22	1489.0
23	1651.0
24	2023.0
25	2527.0
26	3164.0
27	4187.0
28	5129.0
29	6517.0
30	8548.0
31	10584.0
32	13107.0
33	16543.0
34	21672.0
35	29349.0
36	38594.0
37	46727.0
38	29734.0
39	1397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.00086045729394	44.15006375206314	12.814946925429643	33.034128865213276
2	10.389630707374119	46.949678892982575	17.994899834948647	24.665790564694657
3	6.870360375159381	18.84792590680465	44.930342070886034	29.351371647149932
4	9.647681849826736	42.71192672032792	11.673667660103725	35.96672376974163
5	11.555793739584882	43.768532064872986	14.731769142302786	29.943905053239344
6	9.000383294612755	49.420364677445846	17.586964854230715	23.992287173710682
7	6.80699942896925	29.02909127887421	41.20808204069181	22.955827251464733
8	7.0350206118633585	31.726233778424422	15.80894719138917	45.429798418323045
9	6.666979560228099	58.05661808994126	17.44694498548956	17.829457364341085
10-11	6.543386603462167	45.2807828596907	29.489827048083917	18.68600348876321
12-13	5.463512699567424	58.78390006179648	16.14843670554369	19.604150533092405
14-15	6.196074750271826	46.065167906507405	15.488817966348298	32.24993937687247
16-17	6.051165919633289	56.87212040144244	16.299603407410885	20.777110271513386
18-19	19.67513825984246	29.819538638443667	17.492705668849098	33.01261743286478
20-21	30.986631622587783	30.890221293971322	17.373414998552867	20.749732084888024
22-23	3.901391594114472	45.91634790635096	16.882367665579363	33.299892833955205
24-25	3.716393275917365	45.29349416062391	16.392689241937124	34.5974233215216
26-27	3.7731273699381833	45.18991772806652	29.728191679720272	21.308763222275022
28-29	3.849425734674361	43.904261081961636	16.372184163848956	35.87412901951505
30-31	3.7538409781267177	32.14627305320354	30.030532230965278	34.06935373770447
32-33	3.806078147612156	45.85579066765752	21.221692024875814	29.1164391598545
34-35	3.8451082439042517	45.343722557642394	20.820693957938506	29.990475240514847
36-37	3.80525988428724	45.1305191426772	21.088239781248898	29.97598119178666
38-39	3.778189754300331	45.175963516610736	21.407199680848567	29.63864704824036
40-41	3.8326367688272875	45.0728410248788	21.174452738896854	29.920069467397063
42-43	3.8415955441168417	44.5766285173393	21.42066478397259	30.16111115457127
44-45	3.849275498369896	44.90873567669371	21.291684023805193	29.950304801131196
46-47	3.8247551104618536	44.95641953797083	21.20120542951738	30.01761992204993
48-49	3.8564313631899965	44.539884741333104	21.497149832354587	30.106534063122314
50-51	3.8204963222711372	44.74879772197895	21.49569448546021	29.9350114702897
52-53	3.8703507780400472	44.761405615342944	21.28507144826723	30.083172158349775
54-55	3.8018523298836815	44.52905607834855	21.3884260671626	30.280665524605165
56-57	3.7889825188569106	44.64819663988141	21.375141536830867	30.187679304430816
58-59	3.857661935362749	44.466760275821066	21.430107989502215	30.245469799313966
60-61	3.7872935470306954	44.47688802401651	21.479518496425882	30.256299932526915
62-63	3.825319346991137	44.700560861708865	21.58554901086523	29.888570780434765
64-65	3.8171506795736776	44.65454189889508	21.37929011440305	30.14901730712819
66-67	3.751420251763459	44.48355636908359	21.559470659211936	30.20555271994102
68-69	3.8209933644024536	44.667173837792596	21.3979930886526	30.11383970915235
70-71	3.792366376385323	44.51571645646856	21.32502058305286	30.36689658409326
72-73	3.790695263928844	44.26565179893259	21.564700751567965	30.3789521855706
74-75	3.783957148137356	44.58729267830008	21.57045270982491	30.05829746373765
76-77	3.7963370393186464	44.5717582406837	21.519160628550754	30.112744091446896
78-79	3.7922719013970188	44.44644369385667	21.530873083083808	30.230411321662498
80-81	3.8014983836933927	44.55100312701061	21.67043772281662	29.977060766479386
82-83	3.8137872094092065	44.613350699842954	21.412045864795466	30.16081622595237
84-85	3.8649355322573387	44.36550169065262	21.5482427577683	30.221320019321745
86-87	3.9041294127770083	44.525927142734226	21.646563255344613	29.923380189144154
88-89	3.923888492338982	44.69008809926568	21.447526669339304	29.938496739056035
90-91	3.9446100172873693	44.47175744491118	21.6815682225299	29.902064315271552
92-93	4.018961091808308	44.60023632891977	21.590642313832284	29.790160265439635
94-95	4.110451643183307	44.50156935983808	21.491527412462965	29.89645158451565
96-97	4.175190190288072	44.305634326169006	21.591144661959987	29.92803082158293
98-99	4.2860551162008464	44.5740345278045	21.60041145503328	29.539498900961362
100-101	4.584281791941426	44.48485986279617	21.265615344300254	29.66524300096215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	1.0
6	1.0
7	1.5
8	2.0
9	1.5
10	1.0
11	4.5
12	6.0
13	11.0
14	15.5
15	14.0
16	25.5
17	45.5
18	68.0
19	110.5
20	215.5
21	388.5
22	707.5
23	1281.0
24	2194.0
25	3512.0
26	5364.5
27	7757.5
28	10530.5
29	13502.0
30	16211.0
31	18336.0
32	19923.5
33	20317.5
34	19555.5
35	18091.5
36	15952.0
37	13621.0
38	11179.5
39	9058.5
40	7345.5
41	5789.5
42	4489.5
43	3559.0
44	3012.5
45	2516.0
46	2125.5
47	1874.5
48	1606.5
49	1347.5
50	1172.0
51	1067.5
52	958.5
53	892.0
54	868.0
55	803.5
56	772.0
57	772.0
58	756.5
59	741.0
60	707.0
61	641.0
62	604.5
63	580.0
64	509.5
65	434.0
66	359.5
67	302.5
68	256.5
69	223.0
70	162.5
71	118.0
72	85.5
73	58.0
74	49.0
75	32.5
76	21.0
77	15.5
78	12.0
79	9.0
80	4.5
81	3.5
82	4.0
83	1.5
84	0.5
85	0.0
86	0.5
87	0.5
88	0.0
89	1.0
90	1.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.01564467807163698
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.866754276863868E-4
28-29	0.001368909331268236
30-31	0.018186938258277992
32-33	0.003128935614327396
34-35	0.01114683312604135
36-37	0.017600262830591604
38-39	0.0
40-41	0.0072356636081321035
42-43	0.006257871228654792
44-45	0.007626780559923028
46-47	5.866754276863868E-4
48-49	0.030898239191483035
50-51	0.006844546656341179
52-53	0.001368909331268236
54-55	0.0
56-57	9.777923794773112E-4
58-59	0.0019555847589546225
60-61	0.008017897511713954
62-63	0.0
64-65	0.0011733508553727736
66-67	5.866754276863868E-4
68-69	0.004497844945595632
70-71	0.0025422601866410097
72-73	0.004888961897386556
74-75	0.0025422601866410097
76-77	0.004106727993804707
78-79	0.010364599222459499
80-81	9.777923794773112E-4
82-83	0.0052800788491774815
84-85	0.002933377138431934
86-87	0.0
88-89	1.9555847589546225E-4
90-91	0.0
92-93	0.0398939290826743
94-95	1.9555847589546225E-4
96-97	0.005084520373282019
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	255678.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.89946733891125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.27564901763049	98.18308807568363
2	0.5770509309908782	1.1414005940484138
3	0.10421076715649155	0.3091916808826896
4	0.026462869418713467	0.10468654759079703
5	0.0026877237983872174	0.013290722600730553
6	0.0022746494741318337	0.013497697282462376
7	0.003959790271070517	0.0274134804007874
8	0.0	0.0
9	0.0015875851705805938	0.014131019495301775
>10	0.005719568415356464	0.12496559974979354
>50	0.0	0.0
>100	3.9709767388122644E-4	0.06833458226537073
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.007040105132236641	0.0	0.0
2	0.007040105132236641	0.0	0.0
3	0.007040105132236641	0.0	0.0
4	0.007040105132236641	0.0	0.0
5	0.007040105132236641	0.0	0.0
6	0.007040105132236641	0.0	0.0
7	0.007040105132236641	0.0	0.0
8	0.007040105132236641	0.0	0.0
9	0.007040105132236641	0.0	0.0
10-11	0.007040105132236641	0.0	0.0
12-13	0.0072356636081321035	0.0	0.0
14-15	0.007431222084027566	0.0	0.0
16-17	0.007431222084027566	0.0	0.0
18-19	0.007431222084027566	0.0	0.0
20-21	0.007431222084027566	0.0	0.0
22-23	0.008995689891191264	0.0	0.0
24-25	0.014080210264473283	0.0	0.0
26-27	0.01564467807163698	0.0	0.0
28-29	0.015840236547532444	0.0	0.0
30-31	0.01720914587880068	0.0	0.0
32-33	0.01818693825827799	0.0	0.0
34-35	0.018773613685964377	0.0	0.0
36-37	0.019751406065441686	0.0	0.0
38-39	0.020533639969023534	0.0	0.0
40-41	0.022684783203873623	0.0	0.0
42-43	0.024444809486932784	0.0	0.0
44-45	0.02679151119767833	0.0	0.0
46-47	0.029138212908423874	0.0	0.0
48-49	0.03148491461916943	0.0	0.0
50-51	0.033049382426333124	0.0	0.0
52-53	0.0357872010888696	0.0	0.0
54-55	0.03930725365498791	0.0	0.0
56-57	0.04380509860058354	0.0	0.0
58-59	0.04752070964259733	0.0	0.0
60-61	0.051040762208715645	0.0	0.0
62-63	0.059449776672220525	0.0	0.0
64-65	0.06727211570803901	0.0	0.0
66-67	0.07763671493049851	0.0	0.0
68-69	0.09210804214676273	0.0	0.0
70-71	0.11049053888093618	0.0	0.0
72-73	0.13024194494637786	0.0	0.0
74-75	0.15722901461995165	0.0	0.0
76-77	0.18949616314270293	0.0	0.0
78-79	0.23193235241201826	0.0	0.0
80-81	0.2900132197529705	0.0	0.0
82-83	0.36862772706294633	0.0	0.0
84-85	0.47227371928754136	0.0	0.0
86-87	0.5911732726319825	0.0	0.0
88-89	0.7458600270652931	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTGT	15	6.613179E-4	94.9957	1
CATACGG	15	6.613179E-4	94.9957	3
ATAAATC	7340	0.0	80.306305	2
TAAATCA	7930	0.0	77.62574	3
AATAAAT	7280	0.0	76.92303	1
TCATAAA	8965	0.0	74.00058	7
CCGCTTG	45	2.41198E-9	73.885544	2
ATCATAA	9155	0.0	72.827896	6
AATCATA	8580	0.0	72.52003	5
AAATCAT	8575	0.0	71.56527	4
CCCGCTA	20	0.0020725988	71.24677	2
AGCCCAC	35	3.410336E-6	67.854065	1
CGATTGT	70	0.0	67.854065	3
CATAAAC	9790	0.0	67.81308	8
CTTGTTT	50	5.5497367E-9	66.49699	5
CGCTTGT	50	5.5497367E-9	66.49699	3
CCGATTG	65	9.094947E-12	65.76625	2
CCCGATT	65	9.094947E-12	65.76625	1
CCCCGTT	30	1.2995896E-4	63.330467	1
CCGGTTG	30	1.2995896E-4	63.330467	2
>>END_MODULE