##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_ACAGTG_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11370712
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.56700204877232	31.0	28.0	31.0	16.0	34.0
2	30.15259773667528	31.0	30.0	33.0	25.0	34.0
3	26.95908698961432	30.0	25.0	31.0	16.0	34.0
4	31.264847391005926	35.0	28.0	37.0	19.0	37.0
5	32.644754206394445	35.0	32.0	37.0	25.0	37.0
6	34.120558948562525	35.0	33.0	37.0	30.0	37.0
7	34.73869318814671	35.0	35.0	37.0	32.0	37.0
8	34.89220883908287	35.0	35.0	37.0	32.0	37.0
9	36.60564809359942	39.0	35.0	39.0	32.0	39.0
10-11	36.75395139553099	39.0	36.0	39.0	32.5	39.0
12-13	36.7142343596412	39.0	35.0	39.0	32.0	39.0
14-15	37.743234145553615	39.0	37.0	41.0	32.0	41.0
16-17	37.744109788327464	39.0	37.0	41.0	32.0	41.0
18-19	37.88100269967022	39.5	37.0	41.0	33.0	41.0
20-21	37.89610761565409	40.0	37.0	41.0	32.5	41.0
22-23	37.95364096798549	40.0	37.0	41.0	33.0	41.0
24-25	37.79649414284933	40.0	37.0	41.0	32.0	41.0
26-27	37.49273174105578	39.0	37.0	41.0	32.0	41.0
28-29	37.468824186802635	39.0	36.5	41.0	31.5	41.0
30-31	37.513188075116695	39.0	36.5	41.0	31.5	41.0
32-33	37.253736225112455	39.0	36.0	41.0	31.0	41.0
34-35	37.31062859703653	39.0	36.0	41.0	31.0	41.0
36-37	37.21523861184747	39.0	36.0	41.0	31.0	41.0
38-39	37.233933400051306	39.0	36.0	40.0	31.0	41.0
40-41	37.247317920139935	39.0	36.0	40.0	31.0	41.0
42-43	37.055235108033855	39.0	36.0	40.0	31.0	41.0
44-45	36.923584751878735	39.0	35.0	40.0	31.0	41.0
46-47	36.93323300923316	39.0	35.0	40.0	31.0	41.0
48-49	36.817453157271345	39.0	35.0	40.0	31.0	41.0
50-51	36.57221133978773	38.0	35.0	40.0	30.0	41.0
52-53	36.42740782623123	38.0	35.0	40.0	30.0	41.0
54-55	36.200222482994086	38.0	34.0	40.0	30.0	41.0
56-57	35.88582391662396	37.0	34.0	40.0	29.5	41.0
58-59	35.609245773156694	37.0	34.0	39.5	29.0	41.0
60-61	35.33785124352684	36.0	34.0	39.0	29.0	40.5
62-63	35.00175870021873	36.0	33.0	39.0	29.0	40.0
64-65	34.85868130458302	35.0	33.0	38.5	29.0	40.0
66-67	34.54170969389786	35.0	33.0	37.0	29.0	39.5
68-69	34.25078673619504	35.0	33.0	37.0	29.0	39.0
70-71	34.0378778597567	35.0	33.0	36.5	29.0	39.0
72-73	33.87210184373791	35.0	33.0	36.0	29.0	38.5
74-75	33.63364749158163	35.0	33.0	35.5	29.0	37.0
76-77	31.064378735992634	32.0	29.5	34.5	25.0	35.5
78-79	33.089867659818864	34.0	32.5	35.0	29.0	36.0
80-81	33.30083941186105	35.0	33.0	35.0	29.0	36.0
82-83	33.2568589588053	35.0	33.0	35.0	29.0	36.0
84-85	33.18772562402842	35.0	33.0	35.0	29.0	35.5
86-87	33.12736595872293	34.5	33.0	35.0	29.0	35.0
88-89	33.05190923279443	34.0	33.0	35.0	29.0	35.0
90-91	33.02654325449144	34.0	33.0	35.0	29.0	35.0
92-93	32.988110575047614	34.0	33.0	35.0	29.0	35.0
94-95	32.97325323428342	34.0	33.0	35.0	29.0	35.0
96-97	32.994520777613694	34.0	33.0	35.0	29.0	35.0
98-99	33.08444649430585	34.0	33.0	35.0	29.0	35.0
100-101	32.14782878049384	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	2.5519938479487188
2306	2	1.4559896742386336
2306	3	3.060744792246499
2306	4	2.8567111047159344
2306	5	2.8244360112834315
2306	6	1.326463023203793
2306	7	0.6271949864476483
2306	8	0.39206111409990996
2306	9	0.41444656537454705
2306	10-11	0.3542414668794862
2306	12-13	0.3265389928621758
2306	14-15	0.24454507467406472
2306	16-17	0.24261696205073235
2306	18-19	0.11585621579042282
2306	20-21	0.33758830904380943
2306	22-23	0.05760751171609968
2306	24-25	0.18329431583122613
2306	26-27	0.12963010263322872
2306	28-29	0.34186519035270635
2306	30-31	0.2996407327000483
2306	32-33	0.30253761408475555
2306	34-35	0.26773176333778537
2306	36-37	0.24985174456601555
2306	38-39	0.22952246444956614
2306	40-41	0.2098264417900566
2306	42-43	0.05045889738947551
2306	44-45	0.09027031523721263
2306	46-47	0.06816860805603397
2306	48-49	0.1210574130958264
2306	50-51	-0.09972230126658133
2306	52-53	-0.061904428827475044
2306	54-55	-0.13480556444077507
2306	56-57	-0.14422654340224028
2306	58-59	-0.09797019199754686
2306	60-61	-0.17951227204901699
2306	62-63	0.044921703408917324
2306	64-65	-0.06266015172879236
2306	66-67	-0.0640186172801549
2306	68-69	-0.04300984625413662
2306	70-71	-0.04168677095671569
2306	72-73	-0.16626160772263177
2306	74-75	-0.12621627841873106
2306	76-77	-1.3135375943393015
2306	78-79	-0.3353306584473259
2306	80-81	-0.15387165348974463
2306	82-83	-0.16739963228813792
2306	84-85	-0.16340977571236692
2306	86-87	-0.14546973473715497
2306	88-89	-0.18695752711777658
2306	90-91	-0.2756379419286574
2306	92-93	-0.2645785499721356
2306	94-95	-0.25125218972365104
2306	96-97	-0.20433555421075766
2306	98-99	-0.15420367695558923
2306	100-101	-0.587815273486207
2307	1	2.8095588701346337
2307	2	1.6928263522093872
2307	3	4.024161339044465
2307	4	3.4198481686368396
2307	5	3.095103936265808
2307	6	1.5732034699029
2307	7	0.6937554509318176
2307	8	0.4236063146060829
2307	9	0.43800997541399056
2307	10-11	0.44580247327341027
2307	12-13	0.3838087307660558
2307	14-15	0.21144101800539516
2307	16-17	0.2928161277910206
2307	18-19	0.2290814110810402
2307	20-21	0.5319160571360868
2307	22-23	0.30388559181661634
2307	24-25	0.3135832819800797
2307	26-27	0.2888498296827535
2307	28-29	0.39919503851747606
2307	30-31	0.3837171364890466
2307	32-33	0.40288624472685086
2307	34-35	0.4755531694677302
2307	36-37	0.6829585618098832
2307	38-39	0.5375963937352566
2307	40-41	0.42695698204354215
2307	42-43	0.2552465361905476
2307	44-45	0.36704628451433763
2307	46-47	0.23047739165576075
2307	48-49	0.3283746205388738
2307	50-51	0.3305801636234946
2307	52-53	0.4146444372033855
2307	54-55	0.29693534279171274
2307	56-57	0.221965086603376
2307	58-59	0.28026927976031857
2307	60-61	0.17764321139634376
2307	62-63	0.28572657003746116
2307	64-65	0.08394148050555117
2307	66-67	0.17771061829396473
2307	68-69	0.26060553556200006
2307	70-71	0.18690633821765346
2307	72-73	0.11772450776282284
2307	74-75	0.054420176141285026
2307	76-77	-0.3534806584449939
2307	78-79	-0.016518399502849945
2307	80-81	0.10265746527030473
2307	82-83	0.0683296137121161
2307	84-85	0.16139126718236696
2307	86-87	0.15211623159207477
2307	88-89	0.01541472736082028
2307	90-91	0.030935122482496524
2307	92-93	-0.08803236455171515
2307	94-95	-0.13026052138061317
2307	96-97	0.0049049879647355965
2307	98-99	0.05449621725644249
2307	100-101	-0.24155458607015845
2201	1	1.4834915013573173
2201	2	0.6718451509466874
2201	3	1.6425786530496183
2201	4	-0.41038621041124657
2201	5	0.6235406243396753
2201	6	0.3578035993753659
2201	7	0.23954406919344962
2201	8	0.1364310099668984
2201	9	0.22239055238252803
2201	10-11	0.2459303567367428
2201	12-13	0.1316652152458957
2201	14-15	0.1534115420693638
2201	16-17	0.129091914358348
2201	18-19	0.09978693007634831
2201	20-21	0.16257803379305358
2201	22-23	0.17934029997888956
2201	24-25	0.18977141426321964
2201	26-27	0.11975407543466332
2201	28-29	0.23191590552398367
2201	30-31	0.19465673860287325
2201	32-33	0.17641967062776587
2201	34-35	0.11655896437213897
2201	36-37	0.2728521380557041
2201	38-39	0.05783029034977716
2201	40-41	0.10149950653837436
2201	42-43	0.017738192737162706
2201	44-45	-0.037937992226417805
2201	46-47	-0.017594554091203918
2201	48-49	0.031696399564978606
2201	50-51	-0.07974870109873677
2201	52-53	0.04969147219563297
2201	54-55	-0.06558623751579518
2201	56-57	-0.01158455054791574
2201	58-59	-0.09104497055507466
2201	60-61	-0.1536317410107273
2201	62-63	-0.03342547155223485
2201	64-65	-0.08078424929786365
2201	66-67	-0.03923586130835588
2201	68-69	0.0066939446395437585
2201	70-71	-0.18550231682468166
2201	72-73	-0.25780397283269707
2201	74-75	-0.2641651037422079
2201	76-77	-0.9352771340322938
2201	78-79	-0.41849565119113663
2201	80-81	-0.2761715639271429
2201	82-83	-0.24006953034682965
2201	84-85	-0.28525579476445273
2201	86-87	-0.18774270796789239
2201	88-89	-0.2376061455565477
2201	90-91	-0.13997930731559194
2201	92-93	-0.24589048366592436
2201	94-95	-0.34899927187860413
2201	96-97	-0.21901943847060323
2201	98-99	-0.22091464527190396
2201	100-101	-0.5209012472443248
1101	1	0.9490977051657765
1101	2	0.252439665620539
1101	3	1.0450843765650788
1101	4	-0.2202372641035133
1101	5	1.127535385107457
1101	6	0.5037731015349038
1101	7	0.17773199474292767
1101	8	0.1307632993875032
1101	9	0.0629924479730164
1101	10-11	0.08893215885800032
1101	12-13	0.09848350412818974
1101	14-15	0.05248687134964314
1101	16-17	0.02883796747135392
1101	18-19	0.015867011071144077
1101	20-21	0.09765678478527917
1101	22-23	-0.009997817146050636
1101	24-25	0.07937706715085824
1101	26-27	0.03875993580975745
1101	28-29	0.09967284521997044
1101	30-31	0.07111136767378667
1101	32-33	0.09695881649214044
1101	34-35	0.01710609426275056
1101	36-37	0.122175333569146
1101	38-39	0.0520556600283939
1101	40-41	-0.09152643659627557
1101	42-43	-0.11787484272591797
1101	44-45	-0.16509012866097095
1101	46-47	-0.07373908166544396
1101	48-49	0.011890390793745098
1101	50-51	-0.11389606272980757
1101	52-53	-0.0823855971266596
1101	54-55	-0.14279817488387891
1101	56-57	-0.09487471208490206
1101	58-59	-0.05662758243143884
1101	60-61	-0.1393263621893155
1101	62-63	-0.015350566653388853
1101	64-65	-0.13157669998204824
1101	66-67	-0.13398609719170906
1101	68-69	-0.15816696007060926
1101	70-71	-0.2143797807997032
1101	72-73	-0.26590276349267583
1101	74-75	-0.3358095123626583
1101	76-77	-0.9414767281225416
1101	78-79	-0.36633289660337454
1101	80-81	-0.1617776633560979
1101	82-83	-0.20360224749644829
1101	84-85	-0.1397069865057432
1101	86-87	-0.11713942591507731
1101	88-89	-0.21562178666444964
1101	90-91	-0.25132853416223355
1101	92-93	-0.2139095041484822
1101	94-95	-0.3136523458815077
1101	96-97	-0.21559225257620795
1101	98-99	-0.19793413592050513
1101	100-101	-0.4387534930603252
2304	1	2.474877800658671
2304	2	1.291339700741311
2304	3	2.505320340399038
2304	4	3.6427828394646156
2304	5	2.854467175487649
2304	6	1.151562998573091
2304	7	0.4326152165614445
2304	8	0.22225912956419336
2304	9	0.0699786683291137
2304	10-11	0.013675696196507658
2304	12-13	-0.06305557038794518
2304	14-15	-0.03317267129956747
2304	16-17	-0.10791850613988885
2304	18-19	-0.05486513766884116
2304	20-21	0.020379377399386556
2304	22-23	-0.0801571428586314
2304	24-25	-0.13350189186093075
2304	26-27	-0.1194905349544726
2304	28-29	-0.1308741770976951
2304	30-31	0.010848219963108363
2304	32-33	0.11777905143940615
2304	34-35	-0.11004105645390894
2304	36-37	-0.19543404086898164
2304	38-39	-0.1915668838501361
2304	40-41	-0.14342546071804207
2304	42-43	-0.30124215270341637
2304	44-45	-0.1681583032684557
2304	46-47	-0.11818907354848562
2304	48-49	-0.1351677897459922
2304	50-51	-0.3145302905835692
2304	52-53	-0.2785821877951733
2304	54-55	-0.2598633547150868
2304	56-57	-0.26695235481730606
2304	58-59	-0.25924919075094266
2304	60-61	-0.4415344700695698
2304	62-63	-0.21675005312797424
2304	64-65	-0.38941515889609946
2304	66-67	-0.24348933761009306
2304	68-69	-0.2760760752700051
2304	70-71	-0.29446076874404525
2304	72-73	-0.30505967998882255
2304	74-75	-0.2335151210793711
2304	76-77	-0.9091295433166451
2304	78-79	-0.28984630003247247
2304	80-81	-0.1700030566788584
2304	82-83	-0.12673748860321155
2304	84-85	-0.1134543265903929
2304	86-87	-0.10260719191935408
2304	88-89	-0.14697880712056843
2304	90-91	-0.1920233383220804
2304	92-93	-0.17526931725927142
2304	94-95	-0.1483939550982427
2304	96-97	-0.20786482203217815
2304	98-99	-0.192215094691079
2304	100-101	-0.41600354382334004
2202	1	2.229457358742799
2202	2	1.1522060048148468
2202	3	1.8273036805739054
2202	4	3.427623189315092
2202	5	2.312218610214053
2202	6	0.9789773453171549
2202	7	0.43005785487873993
2202	8	0.24629926400933755
2202	9	0.05885104651010664
2202	10-11	0.0020504301388726276
2202	12-13	-0.05395588138715368
2202	14-15	-0.20361911734603666
2202	16-17	-0.19534395565603546
2202	18-19	-0.16096804022061661
2202	20-21	-0.03917555178280452
2202	22-23	-0.12082571841781942
2202	24-25	-0.059256401460054065
2202	26-27	-0.1444812220617493
2202	28-29	-0.2579487880240663
2202	30-31	-0.20792159448404135
2202	32-33	-0.12825903873076783
2202	34-35	-0.21699228076324317
2202	36-37	-0.07955876661125671
2202	38-39	-0.1170021120348892
2202	40-41	-0.1441414716756242
2202	42-43	-0.2513134988709922
2202	44-45	-0.22826633798312912
2202	46-47	-0.22434576882537982
2202	48-49	-0.29302014135525667
2202	50-51	-0.3478410799050522
2202	52-53	-0.29970951341460506
2202	54-55	-0.290519161435455
2202	56-57	-0.3620248785378024
2202	58-59	-0.43962091411270166
2202	60-61	-0.37754296248461117
2202	62-63	-0.3629956266221015
2202	64-65	-0.2865027697015847
2202	66-67	-0.22700964538489643
2202	68-69	-0.18835245785311372
2202	70-71	-0.17446973394548593
2202	72-73	-0.3246411033989105
2202	74-75	-0.23042798097676354
2202	76-77	-0.9132072405422953
2202	78-79	-0.2708259946309184
2202	80-81	-0.20331514184142208
2202	82-83	-0.20163402520093854
2202	84-85	-0.15143864118755346
2202	86-87	-0.27383662807707054
2202	88-89	-0.2606790452440322
2202	90-91	-0.14493632534598788
2202	92-93	-0.1471520012811922
2202	94-95	-0.1771105606898331
2202	96-97	-0.13396897222595072
2202	98-99	-0.14328877989136402
2202	100-101	-0.31324274547756303
1102	1	1.482824100600883
1102	2	0.7779231934084656
1102	3	0.06940535449255947
1102	4	0.236750431046719
1102	5	1.5593013122588246
1102	6	0.6074186555369963
1102	7	0.15961296518975132
1102	8	0.082905076575301
1102	9	-0.06297510979424459
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1213	96-97	0.26124779475288307
1213	98-99	0.2283479699417228
1213	100-101	0.4337003963900017
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	238.0
15	6469.0
16	6693.0
17	6196.0
18	4727.0
19	3646.0
20	4213.0
21	3161.0
22	4005.0
23	6079.0
24	12017.0
25	28186.0
26	63199.0
27	125688.0
28	218283.0
29	330781.0
30	452528.0
31	576799.0
32	714938.0
33	876709.0
34	1099749.0
35	1441717.0
36	2000174.0
37	2357019.0
38	954531.0
39	71144.0
40	1823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.388698966256467	25.702638497923434	13.288517025143193	42.620145510676906
2	21.715516994971537	22.960376947787104	23.05634648238499	32.26775957485637
3	15.00294774650893	17.799700316836567	26.543124625374055	40.65422731128045
4	20.944261412234294	15.744149129013069	14.19364631841269	49.117943140339946
5	25.62754093173392	19.672611316054976	17.350266550351222	37.349581201859884
6	21.196940352096966	28.217408078304647	19.909209397044236	30.676442172554154
7	13.963129231123341	35.93914457946608	23.905643496730626	26.192082692679953
8	12.471889910257685	37.48487194150314	22.922614742541132	27.120623405698048
9	12.78502164748182	38.359662528368695	24.034696683755097	24.82061914039439
10-11	13.125991101772588	35.53081564848263	25.646658388937876	25.696534860806906
12-13	13.085841747405242	38.150152443029306	23.98142185992727	24.782583949638187
14-15	13.343690703082672	35.1625929617741	23.101653814560823	28.3920625205824
16-17	13.46838485175258	35.28558538660623	23.35801582695192	27.888013934689276
18-19	15.047649101304392	33.06665003230129	24.013292048630408	27.87240881776391
20-21	16.450542794667385	32.65513597134464	23.727311239517608	27.16700999447037
22-23	13.092820281311132	33.600158447743226	24.031729381876765	29.27529188906888
24-25	13.26879745937516	34.54292829556403	23.995813654547014	28.19246059051379
26-27	13.392415172354038	34.05837442411941	25.203426911192057	27.345783492334498
28-29	13.413899901387907	33.53921776636719	23.511786969415127	29.53509536282978
30-31	13.243404752364807	32.79451956576235	25.41252318301232	28.549552498860525
32-33	13.267318454238646	33.886276691067714	24.23262789088028	28.613776963813358
34-35	13.567907302385004	33.45663044668205	24.13408691136919	28.841375339563758
36-37	13.627548975865613	33.860467468340296	24.19991040592809	28.312073149865995
38-39	13.174738542495875	33.50706666176832	24.514073072072755	28.804121723663044
40-41	13.372583094635015	33.15148340099597	24.7771977979684	28.69873570640062
42-43	13.460141903826273	33.416671093483416	24.307977208611582	28.815209794078722
44-45	13.440149556031761	32.515705376770526	24.768808214195897	29.275336853001814
46-47	13.612133448299243	32.50188159539877	24.72042530122352	29.16555965507846
48-49	13.487274536398388	33.99141908604011	24.2938521403067	28.2274542372548
50-51	13.47816744488171	33.152680581964	24.171505162187206	29.197646810967086
52-53	13.4157157977763	32.905374584693334	24.49760301872934	29.181306598801033
54-55	13.32136478646982	34.03677037159012	24.433348846393493	28.20851599554657
56-57	13.296367885991996	32.841114726307694	24.198941826229884	29.663575561470424
58-59	13.218045837390513	32.75891652606806	24.954510237091036	29.068527399450396
60-61	13.33645904972189	33.938648672494296	24.72012772830926	28.004764549474547
62-63	13.116435161840315	32.954493049647006	24.56860653459599	29.36046525391669
64-65	13.148569921466525	33.0796692887632	24.79026740854855	28.98149338122173
66-67	13.213787214037012	34.328732470512705	24.28722869868409	28.170251616766194
68-69	13.071433513603814	33.42362735619236	24.31158626379924	29.193352866404588
70-71	12.966115310694487	33.200277455366965	24.77778180640019	29.055825427538363
72-73	13.041060968992891	34.1993344834405	24.766596757555007	27.993007790011603
74-75	12.969369263992334	33.66715805870347	24.430850562228965	28.93262211507523
76-77	12.894994899955451	33.259297369091556	24.864529525405636	28.98117820554736
78-79	12.885874347230189	34.43207537114158	24.585245292182776	28.096804989445456
80-81	12.944811387533365	33.92088452708315	24.40834164690732	28.72596243847616
82-83	12.678876813648948	33.12725290562751	24.887300822877727	29.306569457845814
84-85	12.807332183435014	34.173215743130974	24.826227115188875	28.193224958245132
86-87	12.844387891919723	33.95229056290229	24.68927683544962	28.514044709728363
88-89	12.902814289425004	33.72434512758452	24.75908096569095	28.61375961729952
90-91	12.949931073458675	34.57490095550036	24.880399831183315	27.59476813985765
92-93	13.395855920179066	34.339122368714236	24.361652436626795	27.9033692744799
94-95	13.585244102479377	34.78348116822579	24.07791972156521	27.553355007729618
96-97	14.01968191420182	35.32719966558126	23.820239827985883	26.832878592231037
98-99	14.277980389870262	34.75706559824864	23.12524775642345	27.839706255457646
100-101	14.646855157164564	34.83191162363488	22.567216289011867	27.95401693018868
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4078.0
1	4075.5
2	3789.0
3	2993.5
4	2474.5
5	2483.5
6	2474.0
7	2422.0
8	2378.0
9	2386.5
10	2595.0
11	2806.0
12	2867.0
13	3743.0
14	4754.5
15	5103.5
16	5610.5
17	6682.0
18	8007.0
19	9244.5
20	10709.5
21	12457.5
22	14522.5
23	17185.0
24	20122.0
25	23660.0
26	28064.5
27	34007.0
28	43188.0
29	55840.5
30	75845.5
31	104619.0
32	146266.0
33	204830.0
34	279436.0
35	371817.5
36	475508.0
37	577050.0
38	668613.0
39	739727.0
40	781994.5
41	796131.5
42	788118.0
43	760358.0
44	713456.0
45	654270.0
46	588456.0
47	525320.0
48	471661.0
49	445808.5
50	442866.5
51	412086.5
52	326076.5
53	237716.0
54	185074.5
55	150933.5
56	128485.5
57	112346.0
58	99585.5
59	89452.0
60	81467.5
61	74537.5
62	69103.5
63	63754.0
64	58201.5
65	53684.5
66	49268.0
67	45442.5
68	41992.5
69	38266.5
70	34575.0
71	31360.0
72	28432.0
73	25806.0
74	23542.0
75	21238.5
76	19345.5
77	18048.5
78	16974.0
79	15847.0
80	14847.0
81	14133.5
82	13569.0
83	12713.5
84	11929.5
85	11659.0
86	11428.0
87	11303.5
88	9727.5
89	8127.0
90	7427.5
91	6739.0
92	6139.0
93	5288.0
94	4829.0
95	4606.5
96	4487.0
97	4402.5
98	4373.5
99	4365.0
100	4364.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00379044185998653
3	0.005515174955511361
4	0.012573083799119603
5	4.7568949209840115E-4
6	2.644283104000536E-5
7	1.7637715503119803E-5
8	9.704533137407632E-5
9	1.764907912399855E-5
10-11	8.82769335682971E-6
12-13	8.831004987354221E-6
14-15	3.533932131628546E-5
16-17	5.744838914053984E-5
18-19	2.4314879636482822E-4
20-21	4.8652948039138026E-5
22-23	4.4258950266217584E-4
24-25	0.0
26-27	3.994853740636784E-5
28-29	2.401912562910093E-4
30-31	2.586163845072734E-4
32-33	8.94247218220466E-5
34-35	3.8569441476366467E-4
36-37	3.1493336639833744E-5
38-39	4.9645376307213536E-5
40-41	1.0414269215107772E-4
42-43	0.0023038607890073395
44-45	0.002034449061386855
46-47	0.0012773487696887975
48-49	0.003147823009262734
50-51	0.0020901254721238166
52-53	4.632339977436798E-6
54-55	0.0
56-57	0.0
58-59	1.4075889690067678E-5
60-61	0.0
62-63	1.4204200437745049E-5
64-65	6.661382921175333E-5
66-67	3.348494519255924E-4
68-69	9.623149756996222E-6
70-71	6.293037721920345E-5
72-73	0.0
74-75	1.4719189510399035E-5
76-77	5.641117731067233E-4
78-79	4.985496691574541E-6
80-81	0.0
82-83	1.9275618209656234E-4
84-85	0.0
86-87	0.0
88-89	3.832791071088486E-4
90-91	1.5611898202305535E-5
92-93	2.6164888300260303E-5
94-95	1.0536553992621673E-5
96-97	3.7204868395219027E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	18769.0
5-9	22995.0
10-14	11249.0
15-19	11784.0
20-24	24735.0
25-29	60508.0
30-34	80699.0
35-39	88203.0
40-44	100494.0
45-49	103073.0
50-54	109621.0
55-59	117971.0
60-64	125863.0
65-69	150670.0
70-74	175197.0
75-79	198703.0
80-84	245520.0
85-89	104532.0
90-94	147044.0
95-99	330223.0
100-102	9142859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.0197802723886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07904382457767	43.380103622108415
2	13.567470757965305	15.472284029424785
3	4.813811552474223	8.234474309842982
4	2.0822069975595316	4.7490794192989805
5	0.9147841678046837	2.6080396122441463
6	0.4797549261534405	1.641331228431912
7	0.3990874239158203	1.5929114054807576
8	0.30364630672326715	1.3851076551906405
9	0.2054907541480699	1.0545333884577333
>10	1.0420928682326853	10.60779968130499
>50	0.07333799987808805	2.8072501124553497
>100	0.03655887087817356	3.6360245707310903
>500	0.0016775588095910119	0.6564246404772617
>1k	8.8025151634859E-4	0.8000884914514284
>5k	7.825839791065546E-5	0.310329554318116
>10k+	7.748096508843865E-5	1.0642182787814611
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	51174	0.4500509730613176	No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA	26935	0.23688050493232085	No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT	15624	0.13740564355160872	No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG	14716	0.1294202157261568	No Hit
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA	11996	0.10549911034594844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.2312333651577843E-4	0.0	0.0
2	1.2312333651577843E-4	0.0	0.0
3	1.2312333651577843E-4	0.0	0.0
4	1.2312333651577843E-4	0.0	0.0
5	1.583014326631437E-4	0.0	0.0
6	1.583014326631437E-4	2.6383572110523948E-5	0.0
7	1.6709595669998502E-4	2.6383572110523948E-5	0.0
8	1.7589048073682632E-4	2.6383572110523948E-5	0.0
9	1.7589048073682632E-4	4.397262018420658E-5	8.794524036841316E-6
10-11	1.8468500477366765E-4	4.836988220262724E-5	8.794524036841316E-6
12-13	2.1106857688419159E-4	5.2767144221047897E-5	1.3191786055261974E-5
14-15	2.2865762495787425E-4	5.2767144221047897E-5	1.7589048073682632E-5
16-17	2.5943845908681887E-4	5.2767144221047897E-5	1.7589048073682632E-5
18-19	2.770275071605015E-4	5.2767144221047897E-5	2.6383572110523948E-5
20-21	3.0780834128944605E-4	5.2767144221047897E-5	2.6383572110523948E-5
22-23	3.47383699455232E-4	5.2767144221047897E-5	2.6383572110523948E-5
24-25	3.8695905762101796E-4	5.2767144221047897E-5	2.6383572110523948E-5
26-27	4.309316778052245E-4	7.035619229473053E-5	3.5178096147365264E-5
28-29	4.968906080815344E-4	7.915071633157184E-5	4.397262018420658E-5
30-31	5.188769181736377E-4	7.915071633157184E-5	5.2767144221047897E-5
32-33	5.452604902841617E-4	7.915071633157184E-5	5.7164406239468555E-5
34-35	5.672468003762649E-4	7.915071633157184E-5	6.156166825788921E-5
36-37	5.892331104683682E-4	7.915071633157184E-5	6.156166825788921E-5
38-39	6.288084686341541E-4	7.915071633157184E-5	6.156166825788921E-5
40-41	6.507947787262574E-4	9.673976440525448E-5	6.156166825788921E-5
42-43	6.81575612855202E-4	1.0553428844209579E-4	7.035619229473053E-5
44-45	7.431372811130912E-4	1.0553428844209579E-4	7.035619229473053E-5
46-47	8.046989493709804E-4	1.1432881247893711E-4	7.915071633157184E-5
48-49	9.190277618499176E-4	1.1432881247893711E-4	8.794524036841316E-5
50-51	9.937812161630688E-4	1.2312333651577843E-4	8.794524036841316E-5
52-53	0.0010861237185499026	1.3191786055261975E-4	8.794524036841316E-5
54-55	0.0011828634829551571	1.3191786055261975E-4	8.794524036841316E-5
56-57	0.0012840005093788323	1.3191786055261975E-4	8.794524036841316E-5
58-59	0.0013939320598393488	1.3191786055261975E-4	8.794524036841316E-5
60-61	0.0014950690862630238	1.3191786055261975E-4	8.794524036841316E-5
62-63	0.0016050006367235403	1.3191786055261975E-4	8.794524036841316E-5
64-65	0.001776493855441946	1.3191786055261975E-4	8.794524036841316E-5
66-67	0.0019655761222340342	1.3191786055261975E-4	8.794524036841316E-5
68-69	0.0022030282712287496	1.4071238458946106E-4	9.673976440525448E-5
70-71	0.0026691380451813393	1.495069086263024E-4	9.673976440525448E-5
72-73	0.00380363164593387	1.495069086263024E-4	1.0553428844209579E-4
74-75	0.005619700859541601	1.495069086263024E-4	1.1432881247893711E-4
76-77	0.008315222476833466	1.495069086263024E-4	1.1432881247893711E-4
78-79	0.011125072906604267	1.495069086263024E-4	1.1432881247893711E-4
80-81	0.014352663228125027	1.495069086263024E-4	1.1432881247893711E-4
82-83	0.018019979751487857	1.495069086263024E-4	1.1432881247893711E-4
84-85	0.023015269404413726	1.495069086263024E-4	1.1432881247893711E-4
86-87	0.5656461970015598	1.495069086263024E-4	1.2312333651577843E-4
88-89	1.385823508677381	1.495069086263024E-4	1.275205985341991E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	56785	0.0	58.89312	5
TCATAAA	62930	0.0	54.578503	7
ATCATAA	65070	0.0	53.32266	6
TAAATCA	60210	0.0	51.10657	3
AAATCAT	66820	0.0	49.345707	4
CATAAAC	76770	0.0	44.986053	8
AATAAAT	70050	0.0	43.906788	1
AGAGCAC	10895	0.0	40.796036	94-95
ATAAACA	88580	0.0	39.231567	9
ATAAATC	88210	0.0	34.98211	2
AACCGGG	1225	0.0	30.433353	1
AAGAGCA	16595	0.0	30.058018	94-95
AGCCCCC	1625	0.0	30.04318	1
ACCGGGC	1325	0.0	29.488031	2
AGCCGCC	2005	0.0	29.44041	1
GAGCACA	7300	0.0	28.782797	94-95
CACGGGC	1785	0.0	28.611092	3
AAACGGG	63460	0.0	27.594782	14-15
GCCCGTA	1795	0.0	27.456018	2
AGCCCGT	1010	0.0	27.244377	1
>>END_MODULE