##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_ACAGTG_L001_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	889732
Sequences flagged as poor quality	0
Sequence length	2-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.79568791501261	28.0	23.0	31.0	16.0	33.0
2	28.392762090157486	30.0	28.0	31.0	16.0	33.0
3	24.248917481272315	28.0	16.0	31.0	10.0	33.0
4	29.101103191282416	32.0	25.0	35.0	19.0	37.0
5	31.75196998461847	35.0	32.0	35.0	19.0	37.0
6	33.648329642714266	35.0	33.0	35.0	30.0	37.0
7	34.46102709834568	35.0	35.0	37.0	31.0	37.0
8	34.6680650271473	35.0	35.0	37.0	32.0	37.0
9	36.40886497126424	38.0	35.0	39.0	32.0	39.0
10-11	36.637563603436305	38.5	35.0	39.0	32.0	39.0
12-13	36.541864303184596	38.0	35.0	39.0	32.0	39.0
14-15	37.62269795387961	39.0	36.0	40.0	32.0	41.0
16-17	37.65154538590704	39.0	36.0	40.0	32.0	41.0
18-19	37.776962188440365	39.5	37.0	41.0	32.0	41.0
20-21	37.655051720343415	39.0	37.0	41.0	32.0	41.0
22-23	37.71353734514715	39.0	37.0	40.5	32.0	41.0
24-25	37.64162759157141	39.0	37.0	40.5	32.0	41.0
26-27	37.41897794820895	39.0	36.0	40.0	32.0	41.0
28-29	37.34456503784535	39.0	36.0	40.0	31.5	41.0
30-31	37.42180162671802	39.0	36.0	40.0	31.5	41.0
32-33	37.21766592051284	39.0	36.0	40.0	31.0	41.0
34-35	37.16833519209187	39.0	36.0	40.0	31.0	41.0
36-37	37.12090246138055	39.0	36.0	40.0	31.0	41.0
38-39	37.16980011561128	39.0	36.0	40.0	31.0	41.0
40-41	37.13373614673147	39.0	36.0	40.0	31.0	41.0
42-43	37.01996737277206	39.0	35.0	40.0	31.0	41.0
44-45	36.90572873880035	39.0	35.0	40.0	31.0	41.0
46-47	36.900868002368625	39.0	35.0	40.0	31.0	41.0
48-49	36.77469134657993	38.5	35.0	40.0	31.0	41.0
50-51	36.60669695915101	38.0	35.0	40.0	30.5	41.0
52-53	36.402878222609246	38.0	34.5	40.0	30.5	41.0
54-55	36.19133593817237	38.0	34.0	40.0	30.0	41.0
56-57	35.87430835438078	37.0	34.0	40.0	29.5	41.0
58-59	35.61684553203122	36.5	34.0	39.5	29.0	41.0
60-61	35.41775118041748	36.0	34.0	39.0	29.0	40.5
62-63	34.928460707406366	35.5	33.0	39.0	29.0	40.0
64-65	34.855979475522275	35.0	33.0	38.5	29.0	40.0
66-67	34.55167069163973	35.0	33.0	37.0	29.0	39.5
68-69	34.325730332222804	35.0	33.0	37.0	29.0	39.0
70-71	34.093633818563774	35.0	33.0	36.0	29.0	39.0
72-73	33.983832675584935	35.0	33.0	36.0	30.0	38.5
74-75	33.80856007947719	35.0	33.0	35.5	30.0	37.0
76-77	31.97243615601862	33.0	30.0	34.5	27.0	35.5
78-79	33.366487274996324	34.5	33.0	35.0	29.5	36.0
80-81	33.47175956197957	35.0	33.0	35.0	30.0	36.0
82-83	33.41882594585002	35.0	33.0	35.0	30.0	36.0
84-85	33.333090431232534	35.0	33.0	35.0	30.0	36.0
86-87	33.25996511833546	34.5	33.0	35.0	30.0	35.0
88-89	33.213117211067846	34.0	33.0	35.0	30.0	35.0
90-91	33.17575248649439	34.0	33.0	35.0	30.0	35.0
92-93	33.162193688405345	34.0	33.0	35.0	30.0	35.0
94-95	33.167985919855006	34.0	33.0	35.0	30.0	35.0
96-97	33.17001215007021	34.0	33.0	35.0	30.0	35.0
98-99	33.2224428456465	34.0	33.0	35.0	30.0	35.0
100-101	32.546705311058375	33.5	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2310	1	1.4468235018681526
2310	2	0.7442653027925807
2310	3	-0.8166534422333207
2310	4	-2.0440747361450597
2310	5	-1.099510577445443
2310	6	-0.40362830017826923
2310	7	-0.03399628837171065
2310	8	0.0061389834539227195
2310	9	0.26946151250074735
2310	10-11	0.3265844968095948
2310	12-13	0.2853985847123255
2310	14-15	0.37342732399707046
2310	16-17	0.30571512482779184
2310	18-19	0.25592365598117794
2310	20-21	0.48815124283989064
2310	22-23	0.31281198126092846
2310	24-25	0.2374104050517971
2310	26-27	0.4308462820828822
2310	28-29	0.4773135271304909
2310	30-31	0.39667936497792766
2310	32-33	0.3512777449728901
2310	34-35	0.2448080410466389
2310	36-37	0.3199957029456755
2310	38-39	0.33799076815849816
2310	40-41	0.35206943485537323
2310	42-43	0.4046974911409862
2310	44-45	0.36912971787109683
2310	46-47	0.4157839864498598
2310	48-49	0.39224442101763657
2310	50-51	0.2760226075303933
2310	52-53	0.27378536117755203
2310	54-55	0.28287247192126586
2310	56-57	0.30397749276286845
2310	58-59	0.31258621261136454
2310	60-61	0.35837991684296355
2310	62-63	0.42296470427443467
2310	64-65	0.3475325912661589
2310	66-67	0.2753007223166364
2310	68-69	0.14316372876513128
2310	70-71	0.006110359701551715
2310	72-73	0.07480935415631507
2310	74-75	0.09690161092770921
2310	76-77	-0.02859106318842919
2310	78-79	-0.025481153601305095
2310	80-81	0.1195065359848897
2310	82-83	0.08984488435400806
2310	84-85	0.0384478968570221
2310	86-87	0.031479681957748085
2310	88-89	0.01946728635323325
2310	90-91	-0.004980175503746409
2310	92-93	-0.051067759753465225
2310	94-95	0.07786630362164715
2310	96-97	0.025180039646343744
2310	98-99	0.028232580640029425
2310	100-101	-0.00927385673379888
2311	1	2.142682190284525
2311	2	1.7972892816183546
2311	3	-0.593377289577802
2311	4	-0.7666970345571222
2311	5	-0.7404140230611773
2311	6	-0.20481514206468177
2311	7	0.0316576595153677
2311	8	0.20978318646967864
2311	9	0.26698573542391557
2311	10-11	0.2705720257497859
2311	12-13	0.280270836363286
2311	14-15	0.37722822206330875
2311	16-17	0.3810289823848194
2311	18-19	0.2560273306878429
2311	20-21	0.4166823803880817
2311	22-23	0.3411720195570709
2311	24-25	0.272796437992497
2311	26-27	0.22689693637130404
2311	28-29	0.2911657579893756
2311	30-31	0.33288796065995285
2311	32-33	0.33829219670350597
2311	34-35	0.3742511839879157
2311	36-37	0.4104435246973708
2311	38-39	0.4342350757954634
2311	40-41	0.44891600020221745
2311	42-43	0.28607907725091053
2311	44-45	0.19944457887497435
2311	46-47	0.3085669611069264
2311	48-49	0.2942468175963242
2311	50-51	0.2797947046855995
2311	52-53	0.34132240248964507
2311	54-55	0.2613381300680686
2311	56-57	0.28546893504122295
2311	58-59	0.3122470586654913
2311	60-61	0.2211811479686716
2311	62-63	0.3670949899059792
2311	64-65	0.21592005203287812
2311	66-67	0.18718999433853867
2311	68-69	0.16274057334808845
2311	70-71	0.09343423020909825
2311	72-73	0.12451357405879548
2311	74-75	0.06074121881420069
2311	76-77	0.21745500579518762
2311	78-79	0.12330003703149117
2311	80-81	0.1287834700912427
2311	82-83	0.09935786077964082
2311	84-85	0.12366132091241866
2311	86-87	0.0958436448990696
2311	88-89	0.07101634030904336
2311	90-91	0.06533089665962422
2311	92-93	0.09814726449722144
2311	94-95	0.10762218889977504
2311	96-97	0.07825264026779166
2311	98-99	0.05961732193163982
2311	100-101	0.07164752489239845
2309	1	2.4429509927189486
2309	2	1.393409479395192
2309	3	2.493178917715017
2309	4	1.678314422233445
2309	5	2.006559693207926
2309	6	0.9308245392645773
2309	7	0.5076181697100282
2309	8	0.3901986312430239
2309	9	0.4004743093298657
2309	10-11	0.3057923638170905
2309	12-13	0.29362977959908676
2309	14-15	0.3454140396082863
2309	16-17	0.3771617248115291
2309	18-19	0.2759934234543593
2309	20-21	0.38374407320801396
2309	22-23	0.33970015123428965
2309	24-25	0.37271176883446344
2309	26-27	0.36983252116868925
2309	28-29	0.39548347955852137
2309	30-31	0.3854957031155166
2309	32-33	0.3122503142560049
2309	34-35	0.33394350875158807
2309	36-37	0.3147053326122915
2309	38-39	0.28638598528851134
2309	40-41	0.24546101749656657
2309	42-43	0.2967091073131698
2309	44-45	0.3892453440802299
2309	46-47	0.25534159028301673
2309	48-49	0.29633951080387533
2309	50-51	0.3322835578625316
2309	52-53	0.3592421639716008
2309	54-55	0.2578734064597654
2309	56-57	0.2868979529622706
2309	58-59	0.2881746973730941
2309	60-61	0.2674092450440355
2309	62-63	0.21553807018704418
2309	64-65	0.2314162630766461
2309	66-67	0.2242602184551572
2309	68-69	0.19746921476856727
2309	70-71	0.11250188698484465
2309	72-73	0.09008712890198467
2309	74-75	0.11036030855830603
2309	76-77	0.10360808513392072
2309	78-79	0.14783623633312715
2309	80-81	0.1720142166064278
2309	82-83	0.13691950219240567
2309	84-85	0.13377593299307478
2309	86-87	0.16110959212349485
2309	88-89	0.1664868210029482
2309	90-91	0.15737019668588204
2309	92-93	0.12316915708700549
2309	94-95	0.1623224569202577
2309	96-97	0.1729316574220192
2309	98-99	0.14972998792917735
2309	100-101	0.10652185153082883
2314	1	-0.1942374720575728
2314	2	0.8185266095969403
2314	3	-1.1129798197167986
2314	4	-1.5958227037377775
2314	5	0.5324717742928726
2314	6	0.20903321937840502
2314	7	0.14706990367846373
2314	8	0.1296950737143021
2314	9	0.22603137817947072
2314	10-11	0.15860806360345236
2314	12-13	0.1589633279353464
2314	14-15	0.07727743389995112
2314	16-17	0.11020729509583305
2314	18-19	0.1691285673610068
2314	20-21	0.2621556600356314
2314	22-23	0.21475838992260066
2314	24-25	0.17835971073402845
2314	26-27	0.13974622560019156
2314	28-29	0.19850755314813284
2314	30-31	0.21688490467801813
2314	32-33	0.05194945775903648
2314	34-35	0.16298209270819797
2314	36-37	0.24291987555841388
2314	38-39	0.11190648590488905
2314	40-41	0.19268633565722837
2314	42-43	0.14682946476195013
2314	44-45	0.1565884572484464
2314	46-47	0.13516842361593007
2314	48-49	0.12268792370123549
2314	50-51	0.156288559805688
2314	52-53	0.21062865682711873
2314	54-55	0.12242943384755733
2314	56-57	0.04791283483783104
2314	58-59	0.15729572728315588
2314	60-61	0.09286196902063182
2314	62-63	0.09669546013509489
2314	64-65	0.03821900041792503
2314	66-67	0.09579981663780757
2314	68-69	0.05588295928190945
2314	70-71	0.05582199716128855
2314	72-73	-0.08411511508880665
2314	74-75	-0.08544852707610318
2314	76-77	-0.1849034569299164
2314	78-79	-0.0026575428197403994
2314	80-81	-0.04158844557341723
2314	82-83	-0.010966041665653847
2314	84-85	0.02098000076252049
2314	86-87	0.04094722247487681
2314	88-89	0.07960818334514386
2314	90-91	0.09812782132097908
2314	92-93	0.07205212928731441
2314	94-95	0.05676154449936632
2314	96-97	0.030622857073161924
2314	98-99	0.021018606945837348
2314	100-101	-0.003339954123831035
2315	1	0.8068843079634753
2315	2	1.0850878754351818
2315	3	1.9363543831172407
2315	4	0.8583032204607797
2315	5	1.5356125490642967
2315	6	0.6681092142854297
2315	7	0.2547326615294878
2315	8	0.1692576620731714
2315	9	0.1413470377903181
2315	10-11	0.052407800250477976
2315	12-13	0.025287986867070344
2315	14-15	-0.0875254444000717
2315	16-17	0.02065392604737326
2315	18-19	0.022261696731391112
2315	20-21	0.029936628803312715
2315	22-23	0.09788820979306223
2315	24-25	0.03064804085487083
2315	26-27	-0.015926321578660918
2315	28-29	0.0796677375453072
2315	30-31	0.06249548987054965
2315	32-33	0.017141404977117247
2315	34-35	0.034988297524478185
2315	36-37	0.036375187330243364
2315	38-39	-0.001957153114453547
2315	40-41	0.05350919755408512
2315	42-43	-0.09006499249685618
2315	44-45	0.05896480258225267
2315	46-47	-0.0013947093993991189
2315	48-49	-0.024053324728470216
2315	50-51	-0.023676535497514806
2315	52-53	-0.07851920608165841
2315	54-55	-0.006776646981663248
2315	56-57	-0.07065797394422901
2315	58-59	0.040274541315127976
2315	60-61	-0.01826228412467401
2315	62-63	-0.029129844249972336
2315	64-65	-0.01195897370417498
2315	66-67	0.035247983088318335
2315	68-69	-0.01485718729536245
2315	70-71	0.06239629697181215
2315	72-73	-0.005357560642885062
2315	74-75	-0.012809704741194139
2315	76-77	-0.09116303536703185
2315	78-79	0.0019227408125104262
2315	80-81	0.05013422001496082
2315	82-83	0.06335401476731306
2315	84-85	0.07506712477541555
2315	86-87	0.025182651913432608
2315	88-89	0.054576331488853214
2315	90-91	0.07942363674307984
2315	92-93	0.0931389042246451
2315	94-95	0.09243093047113149
2315	96-97	0.015180216899473464
2315	98-99	0.05100543050550499
2315	100-101	0.012564084576297319
2312	1	-3.147604024433157
2312	2	-1.1023899453751191
2312	3	-2.609852513752813
2312	4	-2.2307931778032746
2312	5	-0.05736528871729263
2312	6	-0.15713833032159386
2312	7	-0.0885502336213051
2312	8	-0.0629668278934119
2312	9	-0.07501147868005376
2312	10-11	-0.14924931588219437
2312	12-13	-0.009390983348119164
2312	14-15	-0.07426152984559309
2312	16-17	-0.09622901223286817
2312	18-19	-0.16858826184504494
2312	20-21	-0.15385128228808753
2312	22-23	-0.2803009887816401
2312	24-25	-0.1455952212789171
2312	26-27	-0.2192353961729907
2312	28-29	-0.13279589051008145
2312	30-31	-0.11799800967897767
2312	32-33	-0.0984604592703846
2312	34-35	-0.1144840200342685
2312	36-37	-0.11154715881816912
2312	38-39	-0.09497694474946883
2312	40-41	-0.1983975249014165
2312	42-43	-0.12885043465104928
2312	44-45	-0.23065246046400034
2312	46-47	-0.13323382729508637
2312	48-49	-0.17856950334093824
2312	50-51	-0.12087412468444825
2312	52-53	-0.10259759102759602
2312	54-55	-0.20115798582467903
2312	56-57	-0.07747406047614902
2312	58-59	-0.2583743849860838
2312	60-61	-0.26168873328818876
2312	62-63	-0.1556924287863879
2312	64-65	-0.11087800091263489
2312	66-67	-0.06986888740520669
2312	68-69	-0.0605475521480372
2312	70-71	-0.07905994088054058
2312	72-73	-0.08389444791849598
2312	74-75	-0.07550941955793888
2312	76-77	0.04966891693030817
2312	78-79	-0.07193178462031824
2312	80-81	-0.12923895881389313
2312	82-83	-0.1747053719793712
2312	84-85	-0.15404083432867566
2312	86-87	-0.07774833899079425
2312	88-89	-0.06073312077639059
2312	90-91	-0.02890431241761604
2312	92-93	-0.025174296056249545
2312	94-95	-0.009305812377796485
2312	96-97	-0.06413250975680995
2312	98-99	-0.03299960884422859
2312	100-101	-0.04407814468837046
2313	1	-1.4144329076434659
2313	2	-0.13303858842677485
2313	3	-0.9600224571393632
2313	4	0.2343697299232126
2313	5	1.2287531800410036
2313	6	0.34094848938686795
2313	7	0.06951796757221729
2313	8	-0.16989232285364864
2313	9	-0.21463798299699022
2313	10-11	-0.12433314438531795
2313	12-13	-0.38897298353319343
2313	14-15	-0.3878947966946882
2313	16-17	-0.2625716338231996
2313	18-19	-0.1899522481146505
2313	20-21	-0.33621876749427315
2313	22-23	-0.2990415100813806
2313	24-25	-0.2758405250207616
2313	26-27	-0.25449935647472444
2313	28-29	-0.33041136880423494
2313	30-31	-0.20640148497747646
2313	32-33	-0.24951706207318125
2313	34-35	-0.31683810202676455
2313	36-37	-0.2572938619659908
2313	38-39	-0.24063957920849077
2313	40-41	-0.24331210955096338
2313	42-43	-0.20497456896317345
2313	44-45	-0.2071273649364045
2313	46-47	-0.2231695488276486
2313	48-49	-0.19961961262264083
2313	50-51	-0.17931104826527644
2313	52-53	-0.12379001539738255
2313	54-55	-0.025903592953945065
2313	56-57	-0.07640236297935132
2313	58-59	-0.15556766136418076
2313	60-61	-0.16177994568784015
2313	62-63	-0.14810246203038702
2313	64-65	-0.12215269713975374
2313	66-67	-0.20653500375771472
2313	68-69	-0.09376549198560724
2313	70-71	-0.08387689620066396
2313	72-73	-0.04764441080097015
2313	74-75	-0.03783144177680953
2313	76-77	-0.06680987894653256
2313	78-79	-0.013398388072893397
2313	80-81	-0.013741736017763628
2313	82-83	0.046250373801811406
2313	84-85	1.6817573291660892E-4
2313	86-87	-0.0498320323309045
2313	88-89	-0.09662718893018507
2313	90-91	-0.053976055798507616
2313	92-93	-0.0366683966653909
2313	94-95	-0.04284920691042515
2313	96-97	-0.04084677970534045
2313	98-99	-0.06439865328631811
2313	100-101	0.06056587635389121
2316	1	-2.083066588700902
2316	2	-4.603150015036356
2316	3	1.663352221587818
2316	4	3.866400279625779
2316	5	-3.406107307382211
2316	6	-1.3833336897507635
2316	7	-0.888049840012556
2316	8	-0.6722143862070311
2316	9	-1.0146505115472522
2316	10-11	-0.8403822899629034
2316	12-13	-0.6451865485957811
2316	14-15	-0.6236652486282566
2316	16-17	-0.8359664071112505
2316	18-19	-0.6207941642560826
2316	20-21	-1.0905999354925768
2316	22-23	-0.7269882529049099
2316	24-25	-0.6704906171679923
2316	26-27	-0.6776608909967337
2316	28-29	-0.9789307960575968
2316	30-31	-1.0700439286455463
2316	32-33	-0.722933597325067
2316	34-35	-0.7196510019577858
2316	36-37	-0.9555986023598564
2316	38-39	-0.8329446380748848
2316	40-41	-0.8509323513131335
2316	42-43	-0.7104251443559377
2316	44-45	-0.7355930752566167
2316	46-47	-0.7570628759335776
2316	48-49	-0.7032762324270863
2316	50-51	-0.7205277214370227
2316	52-53	-0.8800717719592726
2316	54-55	-0.6906752165363486
2316	56-57	-0.699722818204485
2316	58-59	-0.6966361908979195
2316	60-61	-0.4981013157756138
2316	62-63	-0.7693684894358128
2316	64-65	-0.5880982350370516
2316	66-67	-0.5413948436735581
2316	68-69	-0.39008624473468956
2316	70-71	-0.16732793394738366
2316	72-73	-0.06839852266590896
2316	74-75	-0.05640404514823416
2316	76-77	7.35426572500586E-4
2316	78-79	-0.15959014506291425
2316	80-81	-0.2858693022924612
2316	82-83	-0.2500552222501824
2316	84-85	-0.23805961770467832
2316	86-87	-0.22698242204687347
2316	88-89	-0.2337946527926107
2316	90-91	-0.3123920076897022
2316	92-93	-0.27359700262102393
2316	94-95	-0.4448484051239703
2316	96-97	-0.21718812184661118
2316	98-99	-0.21220566582160671
2316	100-101	-0.19460738180745096
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	54.0
15	689.0
16	768.0
17	660.0
18	415.0
19	337.0
20	390.0
21	298.0
22	403.0
23	635.0
24	1280.0
25	2846.0
26	5995.0
27	11160.0
28	18203.0
29	26599.0
30	35646.0
31	44979.0
32	55791.0
33	69415.0
34	88227.0
35	118525.0
36	162611.0
37	175002.0
38	64178.0
39	4541.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.99523002432193	27.810958805572916	14.057716256131059	41.1360949139741
2	21.599522550712127	23.81323280822903	22.98466497626241	31.602579664796433
3	15.143165801635186	17.07894775299782	28.153823843842424	39.62406260152457
4	22.060791435671955	16.40019341707243	12.723244908745377	48.81577023851024
5	24.5067139599696	19.742364102131013	18.97204627987501	36.778875658024376
6	21.11991976425114	28.49518529161525	19.780816669202206	30.6040782749314
7	13.92345252996831	36.03143994496882	24.14876461765734	25.89634290740553
8	12.468125053595044	37.55094349841359	22.80350590560954	27.177425542381833
9	12.845991665951848	38.78526507781003	23.942781296698435	24.425961959539684
10-11	13.123533977842072	35.871625149954134	25.737012208030485	25.267828664173315
12-13	12.920755927533884	38.63236779994047	23.930909023135186	24.515967249390457
14-15	13.294778090752674	35.4949131376942	22.99109198730898	28.219216784244143
16-17	13.438777182975981	35.72545406239578	23.31232370282019	27.523445051808054
18-19	15.161836877114364	33.10482270161322	23.986490689728107	27.74684973154431
20-21	16.79634501683216	32.73490056295793	23.587937423972388	26.880816996237517
22-23	13.010534683152716	33.89759497605394	23.96067172405437	29.131198616738974
24-25	13.1812444491298	34.8036090078969	23.918660729113	28.0964858138603
26-27	13.377435163099364	34.392386671337654	25.256937351062643	26.973240814500343
28-29	13.37965141386595	33.73125309733909	23.46655044473664	29.422545044058314
30-31	13.15811841239834	32.738508473145316	25.589201196342977	28.51417191811337
32-33	13.24463834079037	34.22234274001394	24.19020911171713	28.34280980747855
34-35	13.534840928800637	33.65952432743913	24.098091467015777	28.707543276744456
36-37	13.529708547297997	34.03650818882799	24.251958798987253	28.181824464886756
38-39	13.227507417677362	33.72439644321359	24.43493307041563	28.613163068693414
40-41	13.37534039404525	33.32828831085689	24.718812482893316	28.577558812204547
42-43	13.4253159075883	33.52106593785211	24.33669882390189	28.716919330657703
44-45	13.461389019541395	32.74844507287488	24.704541935559263	29.08562397202446
46-47	13.639738178201611	32.69881462420878	24.698143767423804	28.963303430165805
48-49	13.526854043862576	34.14917324416999	24.284006913219326	28.039965798748113
50-51	13.553016602969665	33.375909594960326	24.13246928335408	28.938604518715934
52-53	13.477685066184769	33.028628567598965	24.51674353972717	28.97694282648909
54-55	13.348832888276954	34.15442463857531	24.450150906271364	28.046591566876373
56-57	13.387661183651895	33.003630892726015	24.181075825382283	29.42763209823981
58-59	13.25665685092401	32.91027962857102	25.004263362940687	28.828800157564288
60-61	13.431138515267413	33.93284280000748	24.77926904786167	27.85674963686344
62-63	13.228592816547232	33.0927705180943	24.57344312494246	29.105193540416007
64-65	13.289428219640746	33.078484142086126	24.79456738092573	28.837520257347393
66-67	13.288303293272449	34.36045505970626	24.374653519084795	27.97658812793649
68-69	13.20417829711229	33.51571346678111	24.42802169883254	28.852086537274058
70-71	13.129293180148775	33.36953520137443	24.72902654659508	28.77214507188171
72-73	13.205860519147326	34.2545915372215	24.79476690552289	27.744781038108286
74-75	13.141171314736056	33.79200314087748	24.473337238591032	28.593488305795432
76-77	13.126505019091116	33.18442366228395	24.895951046155492	28.79312027246944
78-79	13.083670287604733	34.3616670680542	24.6692989238589	27.88536372048217
80-81	13.166294897937034	33.96116921371871	24.49096199787281	28.38157389047144
82-83	12.861688920483816	33.19321670684717	24.910688528981474	29.034405843687537
84-85	13.01722041058953	34.100159079705044	24.977789975767333	27.904830533938085
86-87	13.078706729336478	33.98351519080657	24.775575504399608	28.162202575457346
88-89	13.184187734105418	33.72914482556954	24.818501712210885	28.268165728114152
90-91	13.255565314937447	34.3958339501482	24.93774281945253	27.410857915461822
92-93	13.684654879463471	34.28592379628627	24.482801447019682	27.546619877230576
94-95	13.946744317099446	34.74987405257334	24.107631783488575	27.195749846838634
96-97	14.38163856297151	35.16595071701823	23.952055416639954	26.500355303370306
98-99	14.737889220850164	34.72399630421441	23.222212480694914	27.31590199424051
100-101	15.069069312202348	34.93604235894361	22.630637504296892	27.364250824557157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	257.0
1	257.5
2	251.0
3	222.5
4	203.0
5	205.0
6	208.5
7	211.5
8	209.0
9	213.0
10	227.5
11	238.0
12	240.0
13	330.0
14	434.5
15	456.5
16	504.0
17	613.0
18	734.5
19	837.0
20	969.0
21	1142.5
22	1311.0
23	1513.0
24	1776.0
25	2109.0
26	2496.5
27	3049.5
28	3924.5
29	5038.0
30	6700.5
31	9108.0
32	12499.0
33	17409.5
34	23619.5
35	31124.5
36	39327.0
37	47042.5
38	53969.5
39	59406.0
40	62297.0
41	62912.5
42	61891.5
43	59681.5
44	56084.5
45	50955.0
46	45699.0
47	40858.5
48	36481.5
49	34144.0
50	33295.5
51	30250.5
52	23823.0
53	17668.0
54	13810.5
55	11348.5
56	9794.5
57	8567.5
58	7561.0
59	6746.5
60	6095.0
61	5639.0
62	5247.0
63	4815.5
64	4409.0
65	4035.5
66	3728.5
67	3439.0
68	3175.0
69	2988.0
70	2690.5
71	2398.0
72	2251.5
73	2071.5
74	1853.0
75	1679.0
76	1558.5
77	1460.0
78	1401.5
79	1327.0
80	1252.5
81	1222.0
82	1189.5
83	1134.0
84	1086.5
85	1069.5
86	1050.5
87	1040.0
88	889.0
89	738.5
90	664.5
91	594.5
92	533.0
93	440.0
94	396.0
95	379.5
96	367.0
97	356.0
98	353.0
99	353.0
100	353.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.4957357945988224E-4
3	0.003259921402170883
4	0.006634215234182176
5	3.3780816549897644E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.6958417395266227E-4
18-19	6.220758898654224E-4
20-21	0.0
22-23	0.0
24-25	1.7003274830732398E-4
26-27	0.0
28-29	1.7069118550718041E-4
30-31	0.0
32-33	5.720748525620086E-4
34-35	6.887269742789172E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.8199595512811186E-5
44-45	0.0010517010387884871
46-47	0.006039691917074444
48-49	0.0015299706539859562
50-51	0.008152236518829598
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.4118600807611346E-4
62-63	6.056461972383745E-5
64-65	1.2166560209264836E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0010702243442042945
78-79	5.70389557054483E-4
80-81	0.0
82-83	5.795231297339795E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	6.579354249539116E-5
92-93	3.96369781296367E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
2-3	403.0
4-5	1944.0
6-7	1109.0
8-9	505.0
10-11	346.0
12-13	448.0
14-15	377.0
16-17	367.0
18-19	404.0
20-21	489.0
22-23	887.0
24-25	1459.0
26-27	1615.0
28-29	2320.0
30-31	2396.0
32-33	2740.0
34-35	2985.0
36-37	3010.0
38-39	2943.0
40-41	3042.0
42-43	3397.0
44-45	3069.0
46-47	3025.0
48-49	3210.0
50-51	3369.0
52-53	3458.0
54-55	3360.0
56-57	3523.0
58-59	3375.0
60-61	3744.0
62-63	3519.0
64-65	3850.0
66-67	4132.0
68-69	4465.0
70-71	4407.0
72-73	4899.0
74-75	5700.0
76-77	5135.0
78-79	5557.0
80-81	6467.0
82-83	7437.0
84-85	5187.0
86-87	2376.0
88-89	2639.0
90-91	2930.0
92-93	3423.0
94-95	4272.0
96-97	6158.0
98-99	9666.0
100-101	734194.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.31746880794975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.10638119194917	82.17131004357051
2	3.921691072767293	6.848642758415462
3	0.9275973698133119	2.429863632150303
4	0.41008482984706207	1.432302773551369
5	0.1461652227472095	0.6381388639018246
6	0.14876104127408116	0.7793662548772624
7	0.07437979622851118	0.45462588759872785
8	0.05448543315280446	0.38060240878461077
9	0.03243635962300848	0.2549034737662936
>10	0.16525683670391883	2.504067279629979
>50	0.007386794729425199	0.4081983029642567
>100	0.004328362378444361	0.7336595614627688
>500	7.848858224534164E-4	0.46388971873973983
>1k	2.60802963334352E-4	0.5004290405868929
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	2857	0.3211079291292209	No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA	1538	0.17286104130232474	No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT	972	0.10924637980875139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	0.0
14-15	0.0	0.0	0.0
16-17	0.0	0.0	0.0
18-19	0.0	0.0	0.0
20-21	2.2478678972994115E-4	0.0	0.0
22-23	3.3718018459491175E-4	0.0	0.0
24-25	3.3718018459491175E-4	0.0	0.0
26-27	3.3718018459491175E-4	0.0	0.0
28-29	3.3718018459491175E-4	1.1239339486497057E-4	0.0
30-31	3.3718018459491175E-4	1.1239339486497057E-4	0.0
32-33	3.3718018459491175E-4	1.1239339486497057E-4	0.0
34-35	3.3718018459491175E-4	1.1239339486497057E-4	0.0
36-37	3.3718018459491175E-4	1.1239339486497057E-4	0.0
38-39	3.3718018459491175E-4	1.1239339486497057E-4	0.0
40-41	5.057702768923675E-4	1.1239339486497057E-4	0.0
42-43	5.619669743248529E-4	1.1239339486497057E-4	0.0
44-45	5.619669743248529E-4	1.1239339486497057E-4	0.0
46-47	6.181636717573383E-4	1.1239339486497057E-4	0.0
48-49	7.86753764054794E-4	1.1239339486497057E-4	0.0
50-51	7.86753764054794E-4	1.1239339486497057E-4	0.0
52-53	8.991471589197646E-4	1.1239339486497057E-4	0.0
54-55	8.991471589197646E-4	1.1239339486497057E-4	0.0
56-57	9.553438563522498E-4	1.1239339486497057E-4	0.0
58-59	0.001011540553784735	1.1239339486497057E-4	0.0
60-61	0.0011239339486497058	1.1239339486497057E-4	0.0
62-63	0.0011239339486497058	1.1239339486497057E-4	0.0
64-65	0.001348720738379647	1.1239339486497057E-4	0.0
66-67	0.0016297042255420733	1.1239339486497057E-4	0.0
68-69	0.001966884410136985	1.1239339486497057E-4	0.0
70-71	0.002135474502434441	1.1239339486497057E-4	0.0
72-73	0.003147015056219176	1.1239339486497057E-4	0.0
74-75	0.004832915979193735	1.1239339486497057E-4	0.0
76-77	0.006462620204735809	1.1239339486497057E-4	0.0
78-79	0.008822881496900189	2.2478678972994115E-4	0.0
80-81	0.011520322973659484	2.2478678972994115E-4	0.0
82-83	0.01551028849136594	2.2478678972994115E-4	0.0
84-85	0.019893630891099794	2.2478678972994115E-4	0.0
86-87	0.5849514235747394	2.2478678972994115E-4	0.0
88-89	1.4442551240148718	2.2478678972994115E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	4705	0.0	64.263664	5
TCATAAA	5205	0.0	58.218662	7
ATCATAA	5415	0.0	56.34372	6
CACACGG	150	0.0	56.335075	1
AAATCAT	5620	0.0	52.606342	4
AACCGGG	85	3.6379788E-12	52.323605	1
CACGGGC	180	0.0	51.931477	3
TAAATCA	5155	0.0	51.722797	3
ACCGGGC	100	0.0	48.941875	2
CATAAAC	6260	0.0	48.27558	8
AATAAAT	5575	0.0	45.23293	1
CCAACGG	205	0.0	43.40743	2
ATAAACA	7070	0.0	43.26374	9
CCGGGCA	115	1.8189894E-12	42.577354	3
GCCGGGA	45	0.0013276001	39.548992	2
AGAGCAC	890	0.0	39.52929	94-95
ATACGGC	220	0.0	38.457962	5
ACACGGG	250	0.0	37.373795	2
ATAAATC	6780	0.0	37.07718	2
TACGGCG	230	0.0	36.79418	6
>>END_MODULE