##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_ATCACG_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13657433
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.752826610974406	31.0	28.0	31.0	16.0	34.0
2	30.220572789046013	31.0	30.0	33.0	26.0	34.0
3	27.381493169201708	31.0	25.0	31.0	16.0	34.0
4	31.84380943254792	35.0	32.0	37.0	19.0	37.0
5	32.4978885098577	35.0	32.0	37.0	22.0	37.0
6	34.06259934316518	35.0	33.0	37.0	30.0	37.0
7	34.71011277186777	35.0	35.0	37.0	32.0	37.0
8	34.889559094660065	35.0	35.0	37.0	32.0	37.0
9	36.58463171089335	38.0	35.0	39.0	32.0	39.0
10-11	36.784956735266334	39.0	36.0	39.0	32.5	39.0
12-13	36.71370440449814	39.0	35.0	39.0	32.0	39.0
14-15	37.762396581816546	39.5	37.0	41.0	32.5	41.0
16-17	37.714924978271284	39.0	37.0	41.0	32.0	41.0
18-19	37.84491652463379	39.5	37.0	41.0	32.5	41.0
20-21	37.808795017600005	40.0	37.0	41.0	32.0	41.0
22-23	37.924852051262974	40.0	37.0	41.0	33.0	41.0
24-25	37.78792197880165	40.0	37.0	41.0	32.5	41.0
26-27	37.49284811075559	39.0	36.5	41.0	32.0	41.0
28-29	37.48520694730733	39.0	36.5	40.5	32.0	41.0
30-31	37.54181375407758	39.0	36.5	41.0	32.0	41.0
32-33	37.36848101104451	39.0	36.0	40.0	31.5	41.0
34-35	37.3337119315719	39.0	36.0	40.0	31.0	41.0
36-37	37.304941137712106	39.0	36.0	40.0	31.0	41.0
38-39	37.30256407008312	39.0	36.0	40.0	31.0	41.0
40-41	37.340192706823984	39.0	36.0	40.0	31.0	41.0
42-43	37.16508269979508	39.0	36.0	40.0	31.0	41.0
44-45	37.03260880686135	39.0	35.5	40.0	31.0	41.0
46-47	37.15086673102806	39.0	36.0	40.0	31.0	41.0
48-49	37.0504331520944	39.0	35.5	40.0	31.0	41.0
50-51	36.80791265052734	39.0	35.0	40.0	30.5	41.0
52-53	36.73390899689649	38.0	35.0	40.0	30.5	41.0
54-55	36.606359526942505	38.0	35.0	40.0	30.5	41.0
56-57	36.391801516808414	38.0	34.5	40.0	30.0	41.0
58-59	36.18663823840685	38.0	34.0	40.0	30.0	41.0
60-61	35.988709847619006	37.0	34.0	40.0	30.0	41.0
62-63	35.786265155253595	37.0	34.0	40.0	30.0	41.0
64-65	35.78889272717806	37.0	34.0	39.0	30.0	41.0
66-67	35.56463506556455	36.0	34.0	39.0	30.0	40.5
68-69	35.35442336843505	36.0	34.0	39.0	30.0	40.0
70-71	35.149285321696546	35.5	34.0	38.5	30.0	40.0
72-73	34.89371813227373	35.0	34.0	37.5	30.0	40.0
74-75	34.59520569612725	35.0	33.5	37.0	30.0	39.0
76-77	31.532686182735524	32.5	29.5	34.5	25.5	36.5
78-79	33.836358470927976	34.5	33.0	36.0	29.5	38.5
80-81	34.01366470760958	35.0	33.0	36.0	30.0	37.5
82-83	33.88261462120742	35.0	33.0	36.0	30.0	37.0
84-85	33.73754251692197	35.0	33.0	35.0	30.0	37.0
86-87	33.6013142172782	35.0	33.0	35.0	30.0	36.0
88-89	33.44712441961078	35.0	33.0	35.0	30.0	36.0
90-91	33.36956321906551	35.0	33.0	35.0	30.0	36.0
92-93	33.289810899980694	34.5	33.0	35.0	29.0	36.0
94-95	33.22841849054011	34.0	33.0	35.0	29.0	35.5
96-97	33.238337178773044	34.0	33.0	35.0	30.0	35.0
98-99	33.28940314088842	34.0	33.0	35.0	30.0	35.0
100-101	32.39828310962464	33.5	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1307	1	2.530410101023282
1307	2	1.492362333763321
1307	3	2.8864234208845687
1307	4	2.515643894814577
1307	5	3.1002521178158418
1307	6	1.4995948378590143
1307	7	0.7623357651133631
1307	8	0.5294553904860564
1307	9	0.6109282635314486
1307	10-11	0.47049160994823325
1307	12-13	0.5031010289870324
1307	14-15	0.49725851840105406
1307	16-17	0.5253106837714157
1307	18-19	0.4313256967535395
1307	20-21	0.6636330196014981
1307	22-23	0.4261375136358794
1307	24-25	0.502145480265817
1307	26-27	0.5227823035104819
1307	28-29	0.6586767140163587
1307	30-31	0.6089472002214649
1307	32-33	0.5715641504433293
1307	34-35	0.6037655208408026
1307	36-37	0.6489744743552563
1307	38-39	0.6708731631524358
1307	40-41	0.6043498990543483
1307	42-43	0.5146527263923204
1307	44-45	0.5346878180064252
1307	46-47	0.44029863533209834
1307	48-49	0.48857459572679574
1307	50-51	0.3938218125009527
1307	52-53	0.396310213342673
1307	54-55	0.2972272702372294
1307	56-57	0.27808654749613737
1307	58-59	0.23454933549574974
1307	60-61	0.27295420680531635
1307	62-63	0.3611996226023564
1307	64-65	0.30166855355665234
1307	66-67	0.2852718478509786
1307	68-69	0.23259666321692407
1307	70-71	0.23182763830703124
1307	72-73	0.17622618640901067
1307	74-75	0.16589676389222774
1307	76-77	-0.6530182911129963
1307	78-79	-0.05040832288734265
1307	80-81	0.063534624171659
1307	82-83	0.06572658505497486
1307	84-85	0.005782757198240063
1307	86-87	0.059994607690491364
1307	88-89	0.06344674147575802
1307	90-91	0.035776303734515125
1307	92-93	0.013105027564954241
1307	94-95	0.016716995535794865
1307	96-97	0.03721316557911791
1307	98-99	0.037440707225322
1307	100-101	-0.2883721447732839
1306	1	2.691576386085064
1306	2	1.6524933411985465
1306	3	3.715175442559353
1306	4	2.9015637503305065
1306	5	3.289669643259657
1306	6	1.6960034590121325
1306	7	0.8395205012428093
1306	8	0.5390680446600129
1306	9	0.6170844420853996
1306	10-11	0.5588000001091729
1306	12-13	0.5740942530907986
1306	14-15	0.5037987328078231
1306	16-17	0.6181352329561278
1306	18-19	0.6049896490851694
1306	20-21	0.8251068465506464
1306	22-23	0.6077426205958574
1306	24-25	0.6117363084981307
1306	26-27	0.6579782687776898
1306	28-29	0.6948413515069731
1306	30-31	0.6823094825039391
1306	32-33	0.6961126229259378
1306	34-35	0.8285176723724845
1306	36-37	1.003348620938958
1306	38-39	0.8709428391901426
1306	40-41	0.7524726367546819
1306	42-43	0.6353663415302506
1306	44-45	0.7711266957207314
1306	46-47	0.605153519088681
1306	48-49	0.6572512380102111
1306	50-51	0.6239535175944937
1306	52-53	0.6766487645392161
1306	54-55	0.6706349903417674
1306	56-57	0.5656825215783883
1306	58-59	0.541785748794581
1306	60-61	0.5587211398738461
1306	62-63	0.5708672884918471
1306	64-65	0.3628166042442018
1306	66-67	0.44819388761211343
1306	68-69	0.5115703317653981
1306	70-71	0.38969772552942317
1306	72-73	0.3653964702626098
1306	74-75	0.2948891458880283
1306	76-77	0.2806276370319374
1306	78-79	0.2639137647109564
1306	80-81	0.31411681162922633
1306	82-83	0.2905326928217278
1306	84-85	0.33924767264962696
1306	86-87	0.38463067861363953
1306	88-89	0.2416078362775309
1306	90-91	0.3056854395388129
1306	92-93	0.23196687414817063
1306	94-95	0.17825520909345016
1306	96-97	0.24096180843206128
1306	98-99	0.23309105697639865
1306	100-101	0.034691543277503456
1101	1	1.3473789347741452
1101	2	0.6612556398740068
1101	3	1.4110599573429923
1101	4	-1.065323361944447
1101	5	0.8216084914820314
1101	6	0.4330612811695218
1101	7	0.27820659111254287
1101	8	0.21366842180654544
1101	9	0.31593120972431166
1101	10-11	0.2798946479448148
1101	12-13	0.2843625160473451
1101	14-15	0.3394846078283891
1101	16-17	0.33119130814674946
1101	18-19	0.36221992410796844
1101	20-21	0.41108959681371715
1101	22-23	0.3926473344966155
1101	24-25	0.4034614567276975
1101	26-27	0.42207143189279606
1101	28-29	0.4309820462039653
1101	30-31	0.42819924361843675
1101	32-33	0.4018434851512467
1101	34-35	0.44986032063095394
1101	36-37	0.48313881684041604
1101	38-39	0.42247593360150404
1101	40-41	0.42416308800905966
1101	42-43	0.3139905841233386
1101	44-45	0.3163596906633046
1101	46-47	0.33097238071116664
1101	48-49	0.2993393225168788
1101	50-51	0.27977145934951153
1101	52-53	0.34175547265063955
1101	54-55	0.33448743542454906
1101	56-57	0.29835701333978193
1101	58-59	0.26311082111508455
1101	60-61	0.21550207152667866
1101	62-63	0.30166907336394644
1101	64-65	0.26825997100672083
1101	66-67	0.24287363608207357
1101	68-69	0.24686515148790278
1101	70-71	0.08113535182074827
1101	72-73	0.01971370294177177
1101	74-75	0.012275355259866672
1101	76-77	-0.3441792923239504
1101	78-79	-0.07059229440739045
1101	80-81	0.004190646009334387
1101	82-83	-0.04853043744600427
1101	84-85	-0.08136359390386616
1101	86-87	0.022093797907025703
1101	88-89	-0.08711643966645966
1101	90-91	0.01312088300366554
1101	92-93	-0.05883789868046563
1101	94-95	-0.12080630828633332
1101	96-97	-0.03452703286997405
1101	98-99	-0.020776804292687245
1101	100-101	-0.20824429656353516
1305	1	0.8724942217163019
1305	2	0.3017914120837837
1305	3	0.8334724868145926
1305	4	-0.7099662311406085
1305	5	1.328040729172379
1305	6	0.6028571159234417
1305	7	0.3158958599869237
1305	8	0.22167284246005892
1305	9	0.19240855019209135
1305	10-11	0.1758407967247706
1305	12-13	0.20423782952387626
1305	14-15	0.229241056479502
1305	16-17	0.21700919850160716
1305	18-19	0.20073109837282033
1305	20-21	0.3445703796349946
1305	22-23	0.18006299388542857
1305	24-25	0.2427991335296511
1305	26-27	0.23108988080522153
1305	28-29	0.3198585102373599
1305	30-31	0.29035100372075817
1305	32-33	0.24264092008692018
1305	34-35	0.28845304360994106
1305	36-37	0.3931182326677387
1305	38-39	0.3670960660433593
1305	40-41	0.231222359411845
1305	42-43	0.17853522927795495
1305	44-45	0.18138574888934045
1305	46-47	0.21896499280037318
1305	48-49	0.33825530539241555
1305	50-51	0.22267238787275545
1305	52-53	0.246178269523476
1305	54-55	0.17336239050170832
1305	56-57	0.19645318661457623
1305	58-59	0.20266591719285998
1305	60-61	0.17200808443808313
1305	62-63	0.20138204337688848
1305	64-65	0.10009360155153502
1305	66-67	0.09730369755718726
1305	68-69	0.001937940903452784
1305	70-71	0.008608970061622756
1305	72-73	-0.04338548075664761
1305	74-75	-0.15712362712829275
1305	76-77	-0.4201158644402234
1305	78-79	-0.10165232620447995
1305	80-81	0.006654305410890515
1305	82-83	-0.027600936550520316
1305	84-85	0.01486417926018646
1305	86-87	0.05675974363692404
1305	88-89	-0.02389868863038913
1305	90-91	-0.06226350252270407
1305	92-93	-0.007208695593398318
1305	94-95	-0.10117801110395419
1305	96-97	0.0037101379620096964
1305	98-99	-0.016505002137996883
1305	100-101	-0.15101559795873953
1102	1	2.392277334192535
1102	2	1.273872637738645
1102	3	2.305709137734407
1102	4	3.1601463641094263
1102	5	3.1214211474196603
1102	6	1.3147206023876947
1102	7	0.5967950962655948
1102	8	0.39571423981132625
1102	9	0.2743469762508184
1102	10-11	0.14880808326687145
1102	12-13	0.15674056964120808
1102	14-15	0.2277262961206361
1102	16-17	0.15883217396348215
1102	18-19	0.20941815416987453
1102	20-21	0.3096984414965718
1102	22-23	0.1649487439886954
1102	24-25	0.14435285526363373
1102	26-27	0.19228078469725318
1102	28-29	0.17316444587039825
1102	30-31	0.30533034264096415
1102	32-33	0.34675350478463685
1102	34-35	0.24654071268471967
1102	36-37	0.15366270673339955
1102	38-39	0.16005258923748755
1102	40-41	0.19506314274380543
1102	42-43	0.07099382477596095
1102	44-45	0.25416477541408966
1102	46-47	0.1641937453357869
1102	48-49	0.15204177248448048
1102	50-51	0.07949379972193782
1102	52-53	0.1947308324251651
1102	54-55	0.08682268582388986
1102	56-57	0.15361779223571403
1102	58-59	0.08673602419155912
1102	60-61	-0.07310656694638595
1102	62-63	0.1043006569686824
1102	64-65	-0.14423830327326925
1102	66-67	0.035848300422905766
1102	68-69	-0.042460208742923555
1102	70-71	-0.06558248848893555
1102	72-73	-0.03430996711679768
1102	74-75	-0.009399962204675205
1102	76-77	-0.3564376351285894
1102	78-79	-0.06964747194142973
1102	80-81	0.0044022065015383305
1102	82-83	0.08102787517325538
1102	84-85	0.06036576011310757
1102	86-87	0.0540190162700398
1102	88-89	0.05726331205103463
1102	90-91	-0.014836200999198468
1102	92-93	0.04958216106426505
1102	94-95	-0.001127867820549966
1102	96-97	-0.044468030711563244
1102	98-99	-0.020367788067041204
1102	100-101	-0.14388504195729723
1304	1	2.1097720971673084
1304	2	1.0871339544315681
1304	3	1.496588679015911
1304	4	2.894101991083378
1304	5	2.5363929417450777
1304	6	1.069667253803729
1304	7	0.4922475988476549
1304	8	0.3215680918228472
1304	9	0.16611162977607563
1304	10-11	0.09875279282810823
1304	12-13	0.03993148661820811
1304	14-15	0.013686353319172895
1304	16-17	-0.02603766159406007
1304	18-19	0.05604167202064758
1304	20-21	0.18835892167901847
1304	22-23	0.048910662367447344
1304	24-25	0.08212061332890386
1304	26-27	0.05567229680066532
1304	28-29	-0.059210699809874257
1304	30-31	0.007643340367010865
1304	32-33	0.04654189671750686
1304	34-35	0.026540931476795038
1304	36-37	0.14575689266501257
1304	38-39	0.14281492680093777
1304	40-41	0.12412703763087762
1304	42-43	0.03690336193441368
1304	44-45	0.08269737112119202
1304	46-47	-0.014219114788780018
1304	48-49	-0.019045365725276042
1304	50-51	0.023623255919254404
1304	52-53	-1.8025553259803928E-5
1304	54-55	0.05276299984927846
1304	56-57	-0.007726042850400461
1304	58-59	-0.07728765071215804
1304	60-61	-0.02883113287532524
1304	62-63	-0.07668271674522487
1304	64-65	0.0037701197921791163
1304	66-67	-0.0023428661675666262
1304	68-69	0.041403264912005966
1304	70-71	-0.01733167435030225
1304	72-73	-0.10527266077875197
1304	74-75	-0.026578263199262153
1304	76-77	-0.42061391879061816
1304	78-79	-0.060799504078360656
1304	80-81	-0.02940373491465209
1304	82-83	-0.023170567108373064
1304	84-85	-0.015201155311807213
1304	86-87	-0.07535540111125982
1304	88-89	-0.0575433523252471
1304	90-91	-0.0066939243851038555
1304	92-93	7.40690251618048E-4
1304	94-95	2.3471456361789933E-4
1304	96-97	-0.02820436938430504
1304	98-99	0.007091337698476252
1304	100-101	-0.022651159774660812
1103	1	1.4696965073601227
1103	2	0.7779448154045348
1103	3	-0.05999734945608992
1103	4	-0.10760010837292455
1103	5	1.8408292599930363
1103	6	0.7964652214818386
1103	7	0.33953947371173854
1103	8	0.18817213106613195
1103	9	0.1600593453407626
1103	10-11	0.10291639269285469
1103	12-13	-0.023753982870594825
1103	14-15	-0.052266380906388576
1103	16-17	-0.0304264537456973
1103	18-19	0.06572901699841083
1103	20-21	0.10602486682542889
1103	22-23	-0.04428030484088197
1103	24-25	-0.009998314256883134
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1213	1	2.266424776193716
1213	2	1.4848042281799003
1213	3	3.252871328101236
1213	4	0.7928414254583167
1213	5	2.5070460124212204
1213	6	1.3957990074546416
1213	7	0.9323621913738194
1213	8	0.6685931591634784
1213	9	0.8158646335927457
1213	10-11	0.829965838983675
1213	12-13	0.8330370950261496
1213	14-15	1.0483709298125632
1213	16-17	1.134179233927071
1213	18-19	0.8983069793078542
1213	20-21	1.0539621028625632
1213	22-23	0.981821728858634
1213	24-25	0.9849290538414195
1213	26-27	0.9036716882523308
1213	28-29	1.0949826598061492
1213	30-31	1.0334226398982906
1213	32-33	1.0747042216541658
1213	34-35	1.1514326540864133
1213	36-37	-1.8347045544183374
1213	38-39	-1.1977411100521493
1213	40-41	0.5567815446202999
1213	42-43	1.040245953272148
1213	44-45	1.1685387190152952
1213	46-47	0.967782960341161
1213	48-49	0.9334377830203167
1213	50-51	1.2076757733359003
1213	52-53	1.1983394164087287
1213	54-55	1.0743084293502534
1213	56-57	1.0076434087732906
1213	58-59	1.1184570671009837
1213	60-61	1.210163381496102
1213	62-63	1.0543241362067093
1213	64-65	0.9845931943999773
1213	66-67	0.9518272418476101
1213	68-69	0.8497925064446861
1213	70-71	0.6931122103140908
1213	72-73	0.5780097216590434
1213	74-75	0.7426502746640651
1213	76-77	1.142723021975069
1213	78-79	0.6545945792405874
1213	80-81	0.5435364241409957
1213	82-83	0.5340537585082075
1213	84-85	0.45914546682038804
1213	86-87	0.3979362046534831
1213	88-89	0.4035950275895672
1213	90-91	0.4280262559247845
1213	92-93	0.41704821210831255
1213	94-95	0.39459067867397835
1213	96-97	0.4029996864826302
1213	98-99	0.4159919572960291
1213	100-101	0.34983589522767033
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	317.0
15	8709.0
16	7899.0
17	8183.0
18	6594.0
19	5222.0
20	6046.0
21	4820.0
22	5816.0
23	8353.0
24	15283.0
25	32884.0
26	69763.0
27	135023.0
28	232113.0
29	349982.0
30	482828.0
31	622950.0
32	776100.0
33	967525.0
34	1228799.0
35	1606236.0
36	2197784.0
37	2809033.0
38	1842444.0
39	225354.0
40	1373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.995985482777034	27.46459016126969	15.678663772320903	40.86076058363237
2	18.531643092957705	26.98770953058734	25.982363982596546	28.498283393858415
3	12.944879839086266	17.00545568105051	33.40554403595599	36.64412044390723
4	18.891984661089882	20.989430777251314	15.318576941296467	44.800007620362344
5	21.585348586235558	24.605047602348534	19.216455879148654	34.59314793226726
6	17.6426699224333	31.44786332240003	22.422352916822476	28.487113838344197
7	11.881984155542662	34.41880286697756	31.684206451705784	22.015006525773998
8	10.375080032176738	37.20235066251174	26.32380182838941	26.098767476922113
9	10.529414051885416	43.72399211510204	27.350083780164553	18.396510052847987
10-11	10.80035519466686	39.839291139966434	30.223154670513935	19.137198994852767
12-13	10.295594495880648	43.863324379267446	26.38947711734019	19.451604007511722
14-15	10.865314667487256	39.96571842054585	25.916057401320902	23.252909510645996
16-17	10.920787749488369	42.97198652759028	25.97395304190364	20.133272681017704
18-19	14.631651710969221	34.72793762328211	26.85406804481831	23.786342620930366
20-21	17.98710413538326	35.003277401446695	26.782258569272898	20.227359893897145
22-23	10.162384452169055	39.04703443884604	26.484830271301373	24.305750837683526
24-25	10.232478032174061	38.68729849510041	26.24666037527794	24.833563097447588
26-27	10.333911570560565	38.82525592608118	29.82792682080484	21.01290568255342
28-29	10.349565867703754	38.491732063595535	25.888609130264665	25.270092938436044
30-31	10.214758961218207	34.980358118837394	30.048857284748433	24.756025635195968
32-33	10.300363067146186	39.08789718060024	27.652824474031007	22.958915278222573
34-35	10.467516621229718	38.82039698092994	27.40224577544017	23.309840622400174
36-37	10.40987840100477	38.49013709038795	27.66102732721425	23.438957181393032
38-39	10.427640946502018	38.680984384553476	27.69023352757062	23.20114114137389
40-41	10.431782890229432	38.722581779487435	27.47910658851095	23.36652874177219
42-43	10.375153065217393	38.442201975402135	27.685775130291525	23.496869829088947
44-45	10.48803020707578	38.42414604228278	27.725047569726573	23.362776180914874
46-47	10.519410768666377	38.25706407984603	27.625819241594552	23.597705909893037
48-49	10.434011006493394	38.37234768818075	27.722832098711542	23.47080920661431
50-51	10.41557652270578	38.410711742067924	27.68557084876831	23.488140886457984
52-53	10.460804129494834	38.492569581200186	27.707595909993437	23.33903037931154
54-55	10.335783564366826	38.4138744404882	27.874886219449646	23.37545577569533
56-57	10.322479919913295	38.33871480836248	27.845781233155957	23.493024038568258
58-59	10.382900694908654	38.49679833723973	27.798883000761503	23.32141796709011
60-61	10.307872851055492	38.56441592790322	27.814425233873735	23.313285987167557
62-63	10.304825855381686	38.65801254061539	27.789264163264022	23.247897440738903
64-65	10.328322976352212	38.53439178891996	27.73161773098738	23.40566750374045
66-67	10.28176982681255	38.46512258106791	27.965679506316967	23.287428085802585
68-69	10.281024969718064	38.587680477428655	27.973886895326462	23.157407657526825
70-71	10.300665693336466	38.41455485272827	27.982298662677607	23.302480791257654
72-73	10.289399994972728	38.36833220219692	28.05809114445869	23.284176658371667
74-75	10.23396837047574	38.563770423070345	28.03855787158141	23.16370333487251
76-77	10.296054346834222	38.45406379137282	27.98635055598285	23.26353130581012
78-79	10.285644277157555	38.45382414716876	28.04686955802006	23.21366201765363
80-81	10.323068951992823	38.56386258374931	28.068540324734176	23.044528139523695
82-83	10.299854471741222	38.458941510459944	27.929614539839847	23.31158947795899
84-85	10.245747818082176	38.40965678930189	28.098592324172728	23.2460030684432
86-87	10.324375013580015	38.72135919755426	28.006258006955235	22.948007781910494
88-89	10.46145074134739	38.58220077052499	27.89196048244976	23.064388005677856
90-91	10.487378526557652	38.504038975680174	27.903635109873935	23.104947387888235
92-93	10.875264154033346	38.50060113963157	27.780301111452015	22.843833594883073
94-95	10.991829023923925	38.838098858788406	27.45762832264978	22.71244379463789
96-97	11.209245586816117	38.96274547513361	27.298822719757972	22.529186218292303
98-99	11.359184640054103	38.833291014614154	27.10933806267739	22.69818628265435
100-101	11.478961128264894	38.88587190701683	26.704018003656994	22.931148961061286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9050.0
1	8904.0
2	8138.5
3	6545.0
4	5578.5
5	5665.0
6	5925.0
7	6214.5
8	6477.5
9	7122.0
10	8580.5
11	10754.5
12	13690.5
13	19446.0
14	27287.5
15	35715.5
16	46271.0
17	59670.5
18	76321.5
19	96719.5
20	122274.5
21	155631.0
22	198008.0
23	249614.0
24	310149.0
25	377114.5
26	448251.5
27	519749.5
28	588383.5
29	647977.5
30	694954.0
31	727591.0
32	745862.5
33	750088.5
34	736817.0
35	713714.0
36	682303.5
37	642124.0
38	600394.0
39	555423.0
40	509463.5
41	465816.5
42	423201.5
43	381979.5
44	343977.5
45	310657.0
46	279448.0
47	250349.5
48	223472.0
49	198777.5
50	178993.5
51	161349.5
52	142603.5
53	126331.0
54	113314.0
55	102336.5
56	93552.0
57	86092.5
58	80323.5
59	75799.5
60	71322.5
61	66893.5
62	62905.0
63	58522.0
64	54066.0
65	50392.0
66	46534.5
67	42855.5
68	39636.0
69	36572.0
70	33167.5
71	30136.0
72	27876.0
73	25822.5
74	24043.5
75	22058.0
76	20299.0
77	19340.5
78	18552.0
79	17479.0
80	16556.0
81	15916.0
82	15360.0
83	14460.0
84	13626.0
85	13426.0
86	13209.5
87	13054.0
88	11035.5
89	9009.0
90	8311.5
91	7638.5
92	6947.0
93	5959.5
94	5520.0
95	5350.0
96	5227.0
97	5130.0
98	5090.5
99	5079.5
100	5078.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006011384727963607
3	0.0028049552060371427
4	0.016725362946969392
5	2.0541928350047373E-4
6	3.670370486462829E-5
7	0.0
8	7.3482998606027515E-6
9	0.0
10-11	1.4704807063924626E-5
12-13	0.0
14-15	6.989479068239166E-5
16-17	1.1408176762854034E-4
18-19	4.3812990838961336E-4
20-21	0.0
22-23	7.373000617341342E-6
24-25	0.0
26-27	4.8066246824022745E-5
28-29	1.4822301174856352E-5
30-31	1.114496083382139E-5
32-33	3.35303864470178E-5
34-35	1.1212680974072506E-4
36-37	0.0
38-39	0.0
40-41	2.2647508032410548E-4
42-43	0.002159136054662961
44-45	0.0028400165093543377
46-47	5.455476804666958E-4
48-49	0.0051763754207623655
50-51	0.0019871070193191002
52-53	7.715566266819019E-6
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	9.084023544683147E-5
66-67	0.0
68-69	1.593680991122958E-5
70-71	0.0
72-73	0.0
74-75	4.855011356883024E-5
76-77	3.1775002996219836E-4
78-79	0.0
80-81	0.0
82-83	3.362740149984023E-4
84-85	0.0
86-87	4.202712279207358E-6
88-89	8.435880956728444E-6
90-91	0.0
92-93	5.1057592070347484E-5
94-95	8.558594103710646E-6
96-97	6.468094721366338E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	26775.0
5-9	28604.0
10-14	11451.0
15-19	15921.0
20-24	30757.0
25-29	76107.0
30-34	101086.0
35-39	108914.0
40-44	118021.0
45-49	118076.0
50-54	121906.0
55-59	126801.0
60-64	126677.0
65-69	139888.0
70-74	169083.0
75-79	190113.0
80-84	220648.0
85-89	107269.0
90-94	153927.0
95-99	344154.0
100-102	1.1321255E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.97230060714634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.58431140008548	73.43432103772014
2	7.334270714216129	12.024140874398288
3	1.42208299101724	3.4971424328392495
4	0.6172636419242835	2.023940832387172
5	0.3049110511445496	1.2497130171430995
6	0.17029198393089778	0.8375535418662533
7	0.11767761109458097	0.6752413157963096
8	0.08201703226877209	0.5378499859233445
9	0.061053844916492635	0.4504251715845145
>10	0.2934642505493676	3.903326321052806
>50	0.007718242470570943	0.4461725425337086
>100	0.004696648783134923	0.7331752444399307
>500	1.8218732626242883E-4	0.10731920423725354
>1k	5.840027219472115E-5	0.0796784780779494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.0983030266375826E-4	0.0	7.3220201775838846E-6
2	1.2447434301892602E-4	0.0	7.3220201775838846E-6
3	1.3179636319650992E-4	0.0	7.3220201775838846E-6
4	1.3179636319650992E-4	0.0	7.3220201775838846E-6
5	1.3179636319650992E-4	0.0	7.3220201775838846E-6
6	1.391183833740938E-4	0.0	7.3220201775838846E-6
7	1.391183833740938E-4	0.0	7.3220201775838846E-6
8	1.391183833740938E-4	0.0	7.3220201775838846E-6
9	1.391183833740938E-4	0.0	7.3220201775838846E-6
10-11	1.8671151452838905E-4	0.0	1.0983030266375828E-5
12-13	2.269826255051004E-4	0.0	2.5627070621543597E-5
14-15	2.4894868603785204E-4	0.0	2.9288080710335538E-5
16-17	2.672537364818118E-4	0.0	2.9288080710335538E-5
18-19	2.8189777683697954E-4	7.3220201775838846E-6	2.9288080710335538E-5
20-21	3.0020282728093924E-4	1.4644040355167769E-5	2.9288080710335538E-5
22-23	3.294909079912748E-4	1.4644040355167769E-5	2.9288080710335538E-5
24-25	3.477959584352345E-4	1.4644040355167769E-5	2.9288080710335538E-5
26-27	3.734230290567781E-4	1.4644040355167769E-5	2.9288080710335538E-5
28-29	4.027111097671136E-4	1.4644040355167769E-5	2.9288080710335538E-5
30-31	4.2833818038865725E-4	1.4644040355167769E-5	2.9288080710335538E-5
32-33	4.42982220743825E-4	1.4644040355167769E-5	3.661010088791942E-5
34-35	4.539652510102008E-4	1.4644040355167769E-5	4.7593131154295245E-5
36-37	4.722703014541606E-4	1.4644040355167769E-5	5.125414124308719E-5
38-39	4.869143418093283E-4	1.4644040355167769E-5	5.8576161420671076E-5
40-41	5.271854527860397E-4	1.4644040355167769E-5	5.8576161420671076E-5
42-43	5.601345435851671E-4	1.4644040355167769E-5	5.8576161420671076E-5
44-45	5.821006041179188E-4	1.4644040355167769E-5	5.8576161420671076E-5
46-47	6.443377756273818E-4	2.1966060532751652E-5	5.8576161420671076E-5
48-49	6.809478765153012E-4	2.1966060532751652E-5	5.8576161420671076E-5
50-51	7.505070682023481E-4	2.1966060532751652E-5	5.8576161420671076E-5
52-53	8.090832296230192E-4	2.1966060532751652E-5	5.8576161420671076E-5
54-55	9.298965625531533E-4	2.1966060532751652E-5	5.8576161420671076E-5
56-57	0.001003116764328992	2.1966060532751652E-5	5.8576161420671076E-5
58-59	0.0010946420165487908	2.1966060532751652E-5	5.8576161420671076E-5
60-61	0.0012337603999228845	2.1966060532751652E-5	5.8576161420671076E-5
62-63	0.001343590702586643	3.294909079912748E-5	5.8576161420671076E-5
64-65	0.001464404035516777	3.661010088791942E-5	6.589818159825496E-5
66-67	0.0016145054491572466	3.661010088791942E-5	6.589818159825496E-5
68-69	0.0018085389838632194	3.661010088791942E-5	6.95591916870469E-5
70-71	0.002200267063363957	4.3932121065503304E-5	7.322020177583884E-5
72-73	0.002818977768369795	4.3932121065503304E-5	7.322020177583884E-5
74-75	0.0038879927142970424	5.125414124308719E-5	7.688121186463079E-5
76-77	0.005535447254253416	5.125414124308719E-5	8.054222195342273E-5
78-79	0.007669816136019119	5.125414124308719E-5	8.054222195342273E-5
80-81	0.010126353905598512	5.125414124308719E-5	8.420323204221466E-5
82-83	0.012938009653790723	5.125414124308719E-5	8.786424213100661E-5
84-85	0.016185325602549178	5.125414124308719E-5	9.518626230859049E-5
86-87	0.3694508331104388	5.491515133187913E-5	1.0250828248617437E-4
88-89	0.8912838891466648	5.8576161420671076E-5	1.0616929257496632E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	155200	0.0	59.268814	5
ATCATAA	167095	0.0	57.904	6
CATAAAC	167715	0.0	57.196274	8
TCATAAA	168960	0.0	56.81041	7
TAAATCA	151310	0.0	56.37897	3
AAATCAT	164295	0.0	54.831165	4
ATAAATC	158220	0.0	54.024994	2
ATAAACA	213540	0.0	45.091843	9
AATAAAT	200620	0.0	42.574104	1
AGAGCAC	8870	0.0	38.81637	94-95
AAACGGG	136390	0.0	35.420475	14-15
ACGGGCA	137010	0.0	35.058075	16-17
GGGCAAC	137380	0.0	34.538322	18-19
CAAACGG	136940	0.0	34.40995	14-15
AACGGGC	136540	0.0	33.685345	16-17
CGGGCAA	136735	0.0	33.35775	18-19
GCAACAT	143705	0.0	33.059376	20-21
ACAAACG	151655	0.0	32.431454	12-13
GGCAACA	141790	0.0	31.943111	20-21
CATACCT	154980	0.0	30.194595	24-25
>>END_MODULE