##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_ATCACG_L001_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13551447
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.50100037287531	31.0	28.0	31.0	16.0	34.0
2	30.143738820831715	31.0	30.0	33.0	25.0	34.0
3	26.807506072479473	30.0	25.0	31.0	16.0	34.0
4	30.670092762219962	35.0	28.0	35.0	19.0	37.0
5	32.83536489742805	35.0	32.0	37.0	26.0	37.0
6	34.272846342674136	35.0	33.0	37.0	31.0	37.0
7	34.89278046402412	35.0	35.0	37.0	32.0	37.0
8	35.06253645094027	36.0	35.0	37.0	32.0	37.0
9	36.836021678564755	39.0	37.0	39.0	32.0	39.0
10-11	36.949622886333856	39.0	37.0	39.0	33.0	39.0
12-13	36.98862102181148	39.0	37.0	39.0	33.0	39.0
14-15	38.1850591208902	40.0	37.0	41.0	33.0	41.0
16-17	38.16252616960601	40.0	37.0	41.0	33.0	41.0
18-19	38.288970514000326	40.0	37.5	41.0	33.5	41.0
20-21	38.28580376942371	40.0	38.0	41.0	33.0	41.0
22-23	38.348268780301154	40.0	38.0	41.0	34.0	41.0
24-25	38.24029020365492	40.0	38.0	41.0	33.5	41.0
26-27	38.01164666407858	40.0	37.0	41.0	33.0	41.0
28-29	37.916550145264395	40.0	37.0	41.0	32.5	41.0
30-31	37.97494520381599	40.0	37.0	41.0	33.0	41.0
32-33	37.71223549515469	40.0	37.0	41.0	32.0	41.0
34-35	37.84988978112386	40.0	37.0	41.0	33.0	41.0
36-37	37.67788454897617	40.0	37.0	41.0	32.0	41.0
38-39	37.75174899250632	40.0	37.0	41.0	32.5	41.0
40-41	37.86449195929188	40.0	37.0	41.0	33.0	41.0
42-43	37.7386727153742	40.0	37.0	41.0	32.0	41.0
44-45	37.65703385183069	40.0	37.0	41.0	32.0	41.0
46-47	37.65963975255583	40.0	37.0	41.0	32.0	41.0
48-49	37.59391008638384	40.0	36.0	41.0	32.0	41.0
50-51	37.4906569082041	39.0	36.0	41.0	32.0	41.0
52-53	37.361900376087924	39.0	36.0	40.5	32.0	41.0
54-55	37.21721822453019	39.0	35.5	40.0	31.5	41.0
56-57	37.01465537615189	39.0	35.0	40.0	31.0	41.0
58-59	36.79321810144972	38.0	35.0	40.0	31.0	41.0
60-61	36.64344392534777	38.0	35.0	40.0	31.0	41.0
62-63	36.34592491537417	37.5	35.0	40.0	31.0	41.0
64-65	36.31398444122493	37.0	35.0	40.0	31.0	41.0
66-67	36.05359128087419	37.0	34.0	39.5	31.0	41.0
68-69	35.75758174858517	36.0	34.0	39.0	31.0	41.0
70-71	35.52055098039784	36.0	34.0	39.0	31.0	40.0
72-73	35.317614877382766	35.5	34.0	38.5	31.0	40.0
74-75	35.03079843914303	35.0	34.0	37.0	31.0	39.5
76-77	32.76791866270089	33.5	31.0	35.5	27.5	38.0
78-79	34.33260183782855	35.0	33.5	36.0	30.0	39.0
80-81	34.37765408797455	35.0	34.0	36.0	31.0	37.5
82-83	34.21081100290628	35.0	34.0	36.0	31.0	37.0
84-85	34.03782379932094	35.0	34.0	35.5	31.0	37.0
86-87	33.901373054338976	35.0	34.0	35.0	31.0	36.0
88-89	33.76727966746151	35.0	33.0	35.0	30.0	36.0
90-91	33.678595863997614	35.0	33.0	35.0	30.0	36.0
92-93	33.608359308910394	35.0	33.0	35.0	30.0	36.0
94-95	33.56253818645179	35.0	33.0	35.0	30.0	36.0
96-97	33.53815100808575	35.0	33.0	35.0	30.0	36.0
98-99	33.585681478062156	35.0	33.0	35.0	30.0	35.0
100-101	32.852569581129714	34.5	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	-0.2064233158789328
2306	2	0.23793800851332136
2306	3	-2.5614763742881372
2306	4	-1.0391203794340278
2306	5	0.6513964193704069
2306	6	0.23076725103045703
2306	7	0.12699536526297095
2306	8	0.130799517143096
2306	9	0.3497433672604515
2306	10-11	0.443580513392412
2306	12-13	0.43696633745737046
2306	14-15	0.34799043472722957
2306	16-17	0.44898158582766
2306	18-19	0.35743017679312317
2306	20-21	0.5151865680603436
2306	22-23	0.4610044516498206
2306	24-25	0.4786406286440865
2306	26-27	0.31310794528030783
2306	28-29	0.5026290947833303
2306	30-31	0.46129619773216746
2306	32-33	0.5844234436883724
2306	34-35	0.485206540386784
2306	36-37	-7.504170505202072
2306	38-39	-5.158288455019111
2306	40-41	-0.6594695459666795
2306	42-43	0.16304255572855197
2306	44-45	0.37512448540299204
2306	46-47	0.403048225620104
2306	48-49	0.47456300524610384
2306	50-51	0.4211585558794937
2306	52-53	0.46439484933757313
2306	54-55	0.35346211999523547
2306	56-57	0.28369982380850445
2306	58-59	0.30651560045312465
2306	60-61	0.2929537294782776
2306	62-63	0.31116959630981
2306	64-65	0.3044875567396872
2306	66-67	0.2925865246577217
2306	68-69	0.25770068839819515
2306	70-71	0.23528998116749023
2306	72-73	0.15552747217293472
2306	74-75	0.12320994701976673
2306	76-77	-0.13439919913496112
2306	78-79	0.11704188295511386
2306	80-81	0.10875891921607916
2306	82-83	0.0231351455359885
2306	84-85	0.10216410116452579
2306	86-87	0.09365038107578272
2306	88-89	0.05055189008477612
2306	90-91	-0.0016847103525634566
2306	92-93	0.027601944814364288
2306	94-95	0.04318111717019946
2306	96-97	0.04975631393593716
2306	98-99	0.09542867897915386
2306	100-101	-0.13354430914601778
2307	1	-1.6519383959704328
2307	2	-0.6779811040913266
2307	3	1.2417747129869987
2307	4	2.270474739687298
2307	5	1.8608935595076446
2307	6	1.0018529634247102
2307	7	0.45552715491049867
2307	8	0.2849067107165766
2307	9	0.47200393462944845
2307	10-11	0.4436349179611696
2307	12-13	0.3671601734115555
2307	14-15	0.17956192049193476
2307	16-17	0.25732737781378745
2307	18-19	0.238060623425568
2307	20-21	0.5877911417715467
2307	22-23	0.4827356563089822
2307	24-25	0.44571957993915845
2307	26-27	0.35440401812407885
2307	28-29	0.5674780413674227
2307	30-31	0.5254918639420367
2307	32-33	-2.52095388762244
2307	34-35	-0.05090517538061334
2307	36-37	0.787066035117455
2307	38-39	0.7285907617912599
2307	40-41	0.5201514018814564
2307	42-43	0.37482459480531105
2307	44-45	0.47904823797627927
2307	46-47	0.4020317711544479
2307	48-49	0.51529601554045
2307	50-51	0.5039936985268625
2307	52-53	0.5162324736494739
2307	54-55	0.485820062352353
2307	56-57	0.48829273515401894
2307	58-59	0.5416293054985601
2307	60-61	0.4846847468860531
2307	62-63	0.5106560077511091
2307	64-65	0.4249351772702994
2307	66-67	0.3365008519897543
2307	68-69	0.32406369807057445
2307	70-71	0.2554870261124904
2307	72-73	0.18940163993457304
2307	74-75	0.15972231115074464
2307	76-77	0.26396598786458014
2307	78-79	0.308959013447641
2307	80-81	0.19882984639266255
2307	82-83	0.17642560305815636
2307	84-85	0.25057561557564156
2307	86-87	0.24167140976811652
2307	88-89	0.20335701587292476
2307	90-91	0.18303281925592785
2307	92-93	0.17342245058947725
2307	94-95	0.1677767966810606
2307	96-97	0.16954658401784428
2307	98-99	0.17521428747893708
2307	100-101	0.14666270673021842
2201	1	2.158804630668044
2201	2	1.3485554730445628
2201	3	2.1750844623109487
2201	4	2.254271500860259
2201	5	2.015757857486655
2201	6	0.9081388411966813
2201	7	0.4356199083365624
2201	8	0.23487820243165203
2201	9	0.3502545580081957
2201	10-11	-3.8313700577559047
2201	12-13	-1.917379657624366
2201	14-15	-0.32494792099308967
2201	16-17	0.19015652108500092
2201	18-19	0.2857224659388109
2201	20-21	0.41166279424450636
2201	22-23	0.39370105809565814
2201	24-25	0.4386200950066552
2201	26-27	0.39734585801571143
2201	28-29	-1.5131025965231117
2201	30-31	-0.9274681983526136
2201	32-33	0.2071637498238914
2201	34-35	0.36934380193872585
2201	36-37	0.644664986122983
2201	38-39	0.5975284309188709
2201	40-41	0.4574978224898203
2201	42-43	0.35903206877675586
2201	44-45	0.41467149884722687
2201	46-47	0.2929655649143186
2201	48-49	0.3350390924421234
2201	50-51	0.31004167070381783
2201	52-53	0.3029358369259896
2201	54-55	0.2828756488169759
2201	56-57	0.3214925670957953
2201	58-59	0.3111066386793553
2201	60-61	0.2340008384463843
2201	62-63	0.2371820174693937
2201	64-65	0.26091601433294187
2201	66-67	0.2267462972648957
2201	68-69	0.25422460409627945
2201	70-71	0.22639383024407067
2201	72-73	0.121561272091391
2201	74-75	0.0630248719009785
2201	76-77	-0.1479986111718432
2201	78-79	0.07350533261820402
2201	80-81	0.08568429120597898
2201	82-83	0.08228306497096582
2201	84-85	0.058730638933845114
2201	86-87	0.06030592479906005
2201	88-89	0.056891774815447604
2201	90-91	0.0427034631035923
2201	92-93	0.045134035503338055
2201	94-95	0.02146457906566468
2201	96-97	0.06193825590148805
2201	98-99	0.06211507607786615
2201	100-101	-0.11566858644647482
2304	1	2.307639865189717
2304	2	1.2988011837257432
2304	3	2.1798327120169816
2304	4	2.6174767823596845
2304	5	1.851113617031288
2304	6	0.8622077821665926
2304	7	0.44908610312057107
2304	8	0.2911722209414265
2304	9	0.2829236177117309
2304	10-11	0.19824583606797574
2304	12-13	0.19001427514146485
2304	14-15	0.2183176463553238
2304	16-17	0.23821232148539195
2304	18-19	0.27356900223824
2304	20-21	0.30982247440582
2304	22-23	0.2771955743270169
2304	24-25	0.27787797956172255
2304	26-27	0.2991022490699322
2304	28-29	0.4103748520555257
2304	30-31	-1.1866334383112829
2304	32-33	-0.6466551285549045
2304	34-35	0.05337921658494338
2304	36-37	0.4549459890060703
2304	38-39	0.4344134196089158
2304	40-41	0.2537894226989508
2304	42-43	0.19441597310807168
2304	44-45	0.22197464493898167
2304	46-47	0.20805327839727994
2304	48-49	0.18839845730941107
2304	50-51	0.21339566402629373
2304	52-53	0.20696061168067814
2304	54-55	0.1735711974852947
2304	56-57	0.20517508267874263
2304	58-59	0.2235516695695594
2304	60-61	0.1582970890877604
2304	62-63	0.2616346106197014
2304	64-65	0.20459242465747707
2304	66-67	0.17821959494732909
2304	68-69	0.1637121454757633
2304	70-71	0.1491723207586375
2304	72-73	0.05241944115233821
2304	74-75	0.09028816228328651
2304	76-77	0.02398245164884827
2304	78-79	0.058426040458208206
2304	80-81	-0.008949136101229271
2304	82-83	0.04032609139621712
2304	84-85	0.05867418438439387
2304	86-87	0.05467437593774349
2304	88-89	0.06480717162725114
2304	90-91	0.048369751752431966
2304	92-93	0.060692635592474176
2304	94-95	0.0427584085274475
2304	96-97	0.004871191119896423
2304	98-99	0.048428855630071155
2304	100-101	-0.057112661310910084
2202	1	1.2641305739557218
2202	2	0.5085367214050045
2202	3	1.7514379441870886
2202	4	-0.5324060921504099
2202	5	0.05567930647823971
2202	6	0.05011995341004649
2202	7	0.07846225711329424
2202	8	0.12959786715968136
2202	9	0.010163942823204763
2202	10-11	0.1605872125584611
2202	12-13	0.16268469492159454
2202	14-15	0.19834607570592766
2202	16-17	0.11323332806617259
2202	18-19	0.004845582409139126
2202	20-21	0.11628921715855967
2202	22-23	0.08928573195237988
2202	24-25	0.16329400031792574
2202	26-27	0.15527319323034305
2202	28-29	0.186966127198545
2202	30-31	0.22601500707538946
2202	32-33	0.22208661812133101
2202	34-35	0.15540071190259397
2202	36-37	0.3909207839499871
2202	38-39	0.27576618516498286
2202	40-41	0.19770235538806702
2202	42-43	0.2037171746554307
2202	44-45	0.15624182139172405
2202	46-47	0.1385536258323654
2202	48-49	0.17579925501173221
2202	50-51	0.1294126028290492
2202	52-53	0.10579421690857771
2202	54-55	0.10348158838331045
2202	56-57	0.13906025045485393
2202	58-59	0.06192821583395158
2202	60-61	0.09970302394915365
2202	62-63	0.09270302109224815
2202	64-65	0.0954335943741853
2202	66-67	0.1352155909372783
2202	68-69	-1.230470945252364
2202	70-71	-0.975082565176784
2202	72-73	-0.1618466419172151
2202	74-75	-0.061102715505327865
2202	76-77	-0.11678256393143016
2202	78-79	-0.01964897985723013
2202	80-81	0.0028443175918297925
2202	82-83	0.010709717993151457
2202	84-85	-8.615065590689142E-4
2202	86-87	-0.02373667967693649
2202	88-89	-0.006653645335141789
2202	90-91	-0.008746766127245564
2202	92-93	-0.011085959215222374
2202	94-95	-0.0033136091782850485
2202	96-97	-0.0509159725926267
2202	98-99	-0.04151660476946262
2202	100-101	-0.06590042975223298
2305	1	0.46366620753118326
2305	2	0.03812544766870474
2305	3	0.6131034545197096
2305	4	-1.3664774046911177
2305	5	-0.7959530585372114
2305	6	-0.3963123704353393
2305	7	-0.24858230112326396
2305	8	-0.11778451849519911
2305	9	-0.02441751285508076
2305	10-11	0.08836615777104129
2305	12-13	0.06081964782839577
2305	14-15	0.05114764985614073
2305	16-17	0.0943725792337986
2305	18-19	0.09261351273976715
2305	20-21	-0.03286949747084833
2305	22-23	0.019105567250157662
2305	24-25	0.13916769489014058
2305	26-27	0.12563740526216804
2305	28-29	0.16067189738970455
2305	30-31	0.11423216901731337
2305	32-33	0.309671766756793
2305	34-35	0.16030716506372755
2305	36-37	0.2922988525227552
2305	38-39	0.22455899828946002
2305	40-41	0.11526204314997557
2305	42-43	0.055430931641517134
2305	44-45	-0.011374200818430324
2305	46-47	-0.014545482384825448
2305	48-49	0.006463209942680237
2305	50-51	0.008641417408767893
2305	52-53	-0.02848249398821423
2305	54-55	0.05076356550421934
2305	56-57	0.048240075151220196
2305	58-59	-0.10993975591966887
2305	60-61	-0.004316999779845787
2305	62-63	0.017410759490267935
2305	64-65	0.05966658205683473
2305	66-67	0.08436913411703983
2305	68-69	0.11852908478235236
2305	70-71	0.08867370619670822
2305	72-73	0.020093303969559884
2305	74-75	0.016416672425080492
2305	76-77	-0.037066312724753914
2305	78-79	0.009645512182437699
2305	80-81	-0.00379859117445136
2305	82-83	-0.004037971126855666
2305	84-85	0.021629071166778147
2305	86-87	0.022538562707005383
2305	88-89	0.039214564891011605
2305	90-91	0.01472054170611159
2305	92-93	-0.017171428316174797
2305	94-95	-0.06232356888922652
2305	96-97	-0.058946558699588536
2305	98-99	-0.04991331226485585
2305	100-101	-0.08204591514880377
2203	1	1.4860420776850702
2203	2	0.6535569765443974
2203	3	0.8995100121578474
2203	4	0.332436802454918
2203	5	0.6975388405832916
2203	6	0.3053875300628377
2203	7	0.15738106454075762
2203	8	0.07053403854885687
2203	9	0.01961117553906888
2203	10-11	0.10437911415628065
2203	12-13	0.1296098489186761
2203	14-15	0.05111875179120062
2203	16-17	0.09658825426188855
2203	18-19	0.06399454697447737
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2209	78-79	0.13774763215274533
2209	80-81	0.12415451559715507
2209	82-83	0.07444975893970707
2209	84-85	0.0969548999331522
2209	86-87	0.07873198768210443
2209	88-89	0.0929074041512763
2209	90-91	0.09981100843218371
2209	92-93	0.10483855121850638
2209	94-95	0.11771283869712335
2209	96-97	0.1318196266544831
2209	98-99	0.055717182061599146
2209	100-101	0.18130386261898934
2208	1	1.1157429319546175
2208	2	0.48212673249166116
2208	3	-0.34471543812824024
2208	4	-0.3167395010787537
2208	5	-0.6034586946651146
2208	6	-0.23432500888242913
2208	7	-0.06441910075435686
2208	8	0.017094396699945946
2208	9	0.12105592660496
2208	10-11	0.16891392673058903
2208	12-13	0.1174758406802141
2208	14-15	-0.0058643508573155145
2208	16-17	-0.8670362934374083
2208	18-19	-0.3693062427401088
2208	20-21	-0.003059895657898437
2208	22-23	0.05231698575428112
2208	24-25	0.11751896507997373
2208	26-27	0.01763473545772598
2208	28-29	0.1414044146884379
2208	30-31	0.11224660967164368
2208	32-33	0.2134201073713271
2208	34-35	0.06980096715255968
2208	36-37	0.3105137014357737
2208	38-39	0.21543355400722675
2208	40-41	0.0880146373128241
2208	42-43	0.06768587571537665
2208	44-45	0.022454345383607688
2208	46-47	-0.01012850404630683
2208	48-49	-0.023918589070468954
2208	50-51	0.05443164499607889
2208	52-53	-0.03476339366817882
2208	54-55	0.05906573321119879
2208	56-57	0.0995647462636029
2208	58-59	0.03705168807017856
2208	60-61	0.013349532293062794
2208	62-63	0.052694541600217804
2208	64-65	0.03553570717114951
2208	66-67	0.019835885660782537
2208	68-69	0.09752631290249525
2208	70-71	0.04005322089093255
2208	72-73	0.0688200290241241
2208	74-75	0.0956470534821463
2208	76-77	0.35984137367057656
2208	78-79	0.041779737005796846
2208	80-81	0.028340749251519526
2208	82-83	0.0363786110683364
2208	84-85	0.01952840312552695
2208	86-87	0.015271943869983318
2208	88-89	0.03925741299951824
2208	90-91	0.020193796914753648
2208	92-93	0.05111397975790055
2208	94-95	0.004572290860480166
2208	96-97	0.03409347191202272
2208	98-99	0.0012761510364924789
2208	100-101	0.1289610767354361
2215	1	-0.32452536555610223
2215	2	-0.7092412500300753
2215	3	-2.1043207065994807
2215	4	-2.4469417158894657
2215	5	-1.6889514531900076
2215	6	-0.7326624147941345
2215	7	-0.30037886724979046
2215	8	-0.1626894287177194
2215	9	0.00397040666851467
2215	10-11	0.11453156890176075
2215	12-13	0.09785189520287929
2215	14-15	0.07291243740937858
2215	16-17	0.059036467482442845
2215	18-19	0.027820716150394276
2215	20-21	0.055821080881969465
2215	22-23	0.007053808567043518
2215	24-25	-0.038117502233987466
2215	26-27	0.09991865762106755
2215	28-29	0.1551119657532496
2215	30-31	0.09566947559326877
2215	32-33	0.19852821101667217
2215	34-35	-0.0488372936127206
2215	36-37	0.1918133468469776
2215	38-39	0.09834164816162883
2215	40-41	0.06509132926316141
2215	42-43	0.04096021127065086
2215	44-45	0.024183480828135373
2215	46-47	0.09316174814967582
2215	48-49	0.07098957460169686
2215	50-51	-0.04319948619679792
2215	52-53	0.027877905612633924
2215	54-55	-0.014405660869037717
2215	56-57	-0.025667660581000007
2215	58-59	0.004535242453286514
2215	60-61	0.017726074240933087
2215	62-63	-0.04339290298507592
2215	64-65	-0.0366566922422038
2215	66-67	-0.05672842845881121
2215	68-69	0.02726738251320171
2215	70-71	-0.08833662050862046
2215	72-73	-0.006203946798578386
2215	74-75	0.01983909657400318
2215	76-77	0.2252730449628615
2215	78-79	0.030065227815448736
2215	80-81	-3.316326889049037E-4
2215	82-83	-0.026819031714929054
2215	84-85	-0.012758477360044651
2215	86-87	0.05718562219632162
2215	88-89	0.05876558536329668
2215	90-91	0.046670045944949834
2215	92-93	0.06755655601304511
2215	94-95	0.040938094234249434
2215	96-97	0.023113892009305914
2215	98-99	0.03105356236984136
2215	100-101	0.09064978612784103
2214	1	-0.3991666326029417
2214	2	-0.012284112244621781
2214	3	-2.834620795585735
2214	4	-2.3027159483517607
2214	5	-1.9887009409430298
2214	6	-0.8639369205116125
2214	7	-0.3947987545214531
2214	8	-0.16585397049664152
2214	9	-0.03073268555903752
2214	10-11	0.05076065029920329
2214	12-13	-0.04429219836512743
2214	14-15	0.005498606248359295
2214	16-17	-0.016539456784229856
2214	18-19	-0.0734771712563358
2214	20-21	-0.06390809148180665
2214	22-23	-0.13251976216666606
2214	24-25	-0.0950087811277669
2214	26-27	-0.0689087904261072
2214	28-29	-0.09056207127053995
2214	30-31	-0.05912556370574151
2214	32-33	0.11119246424201634
2214	34-35	-0.029400945320631422
2214	36-37	0.13123546586768242
2214	38-39	0.09064398491377545
2214	40-41	-0.011466974799148488
2214	42-43	-0.0776523165067502
2214	44-45	-0.11418256527382908
2214	46-47	-0.016558073258245543
2214	48-49	-0.03429194659414492
2214	50-51	-0.05579694281124148
2214	52-53	-0.07617495435589205
2214	54-55	-0.07840515211595545
2214	56-57	-0.08710708796516542
2214	58-59	-0.018123045963889695
2214	60-61	-0.01590368090928962
2214	62-63	-0.012597340551693037
2214	64-65	-0.05054941909601496
2214	66-67	-0.08403553568203392
2214	68-69	-0.03948838613337813
2214	70-71	-0.07269676116720092
2214	72-73	-0.01582139046155362
2214	74-75	0.007323383786541626
2214	76-77	0.4214661074229866
2214	78-79	0.06168305753129033
2214	80-81	0.022170436877168243
2214	82-83	0.02974367652098664
2214	84-85	0.01665760928498372
2214	86-87	0.0037339907525719696
2214	88-89	6.992778768193375E-4
2214	90-91	-0.012889250994120971
2214	92-93	0.02854106151417568
2214	94-95	0.08463558077770017
2214	96-97	0.052059090370406125
2214	98-99	-0.0272803453716719
2214	100-101	0.11852501342683297
2213	1	-0.06180048241222735
2213	2	-0.3958502109134052
2213	3	0.1046712281611093
2213	4	0.11036871670874149
2213	5	0.7720333899411145
2213	6	0.2976665381425079
2213	7	0.0964290889847419
2213	8	0.05015132134290923
2213	9	0.05950296436474645
2213	10-11	0.07635494701750645
2213	12-13	-0.05536891159096058
2213	14-15	-0.036321204503870774
2213	16-17	0.01576096727117715
2213	18-19	-0.1072698547130031
2213	20-21	-0.11529775009153553
2213	22-23	-0.14439663921529444
2213	24-25	-0.07199364200912584
2213	26-27	-0.01727860181920704
2213	28-29	0.02357334200612371
2213	30-31	0.013475469146229102
2213	32-33	0.041450714288920665
2213	34-35	-0.13803768791932214
2213	36-37	-0.010791418775717432
2213	38-39	-0.002745086930140417
2213	40-41	-0.1402818577854319
2213	42-43	-0.07755900912784597
2213	44-45	-0.08047114100865116
2213	46-47	-0.09884970941198645
2213	48-49	-0.05134828863690899
2213	50-51	-0.030581907858767465
2213	52-53	-0.05358776886065897
2213	54-55	-0.0710652837581236
2213	56-57	-0.06613705018779825
2213	58-59	-0.04089632886351069
2213	60-61	-0.024146894218958437
2213	62-63	-0.157832020724463
2213	64-65	-0.07895521849926013
2213	66-67	-0.13912405211373624
2213	68-69	-0.011817638228109217
2213	70-71	-0.05065022432548716
2213	72-73	-0.07973267601784784
2213	74-75	-0.00759354740577578
2213	76-77	0.33234529916393285
2213	78-79	0.08588336662234752
2213	80-81	0.08319618287092823
2213	82-83	0.06757462931370384
2213	84-85	0.028715182864793576
2213	86-87	0.06363170721960643
2213	88-89	0.09054081910792888
2213	90-91	0.06513409279985183
2213	92-93	0.02145296940437902
2213	94-95	0.09047374358172533
2213	96-97	0.10677610832200202
2213	98-99	0.07812447134437406
2213	100-101	0.21395584968969672
2212	1	-1.7226364943063004
2212	2	-0.48029458185733986
2212	3	-2.462425083871789
2212	4	-2.871369036974343
2212	5	-0.6197250974799928
2212	6	-0.35064778675491937
2212	7	-0.21248544141870696
2212	8	-0.20653570795872156
2212	9	-0.0693368075728813
2212	10-11	-0.023040112011379676
2212	12-13	-0.14323937635183626
2212	14-15	-0.20535719406751696
2212	16-17	-0.21520940512903053
2212	18-19	-0.1924124880380944
2212	20-21	-0.26309785635696414
2212	22-23	-0.2694412891547344
2212	24-25	-0.19004296609873705
2212	26-27	-0.20443087953388783
2212	28-29	-0.15569627449667678
2212	30-31	-0.14410828124921693
2212	32-33	-0.1609950278483936
2212	34-35	-0.267121976097485
2212	36-37	-0.07916708290145635
2212	38-39	-0.1256281184331769
2212	40-41	-0.18887266466740016
2212	42-43	-0.20815190367576974
2212	44-45	-0.13335606340881867
2212	46-47	-0.1545060262569251
2212	48-49	-0.14019550831555705
2212	50-51	-0.2080700497870751
2212	52-53	-0.14073805047667776
2212	54-55	-0.17822021738660254
2212	56-57	-0.34426340675655354
2212	58-59	-0.2290262262978402
2212	60-61	-0.19155874913145965
2212	62-63	-0.2886639744919748
2212	64-65	-0.22215239405016973
2212	66-67	-0.15548648639432372
2212	68-69	-0.1395313351987113
2212	70-71	-0.18242391775533662
2212	72-73	-0.27834825206961966
2212	74-75	-0.2600815256756306
2212	76-77	-0.035828923769258836
2212	78-79	-0.06832098763599959
2212	80-81	-0.06760728545747696
2212	82-83	-0.01612890429179714
2212	84-85	-0.042215950823610626
2212	86-87	-0.03824226861190283
2212	88-89	-0.04467971184556774
2212	90-91	0.01890829055626142
2212	92-93	-0.0029608675820185226
2212	94-95	0.005858594025184516
2212	96-97	0.022466719253017686
2212	98-99	-0.024354361343860376
2212	100-101	0.05640288986326425
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	275.0
15	8621.0
16	11154.0
17	8402.0
18	5834.0
19	4661.0
20	5328.0
21	4138.0
22	5057.0
23	7284.0
24	13261.0
25	27110.0
26	55723.0
27	105790.0
28	181207.0
29	274815.0
30	381186.0
31	498467.0
32	634704.0
33	810562.0
34	1064935.0
35	1455664.0
36	2150651.0
37	3085870.0
38	2378089.0
39	369370.0
40	3289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.020222785064945	27.509696934947243	15.644218657978001	40.82586162200981
2	18.589879021816717	26.888424950231055	26.01395854999745	28.507737477954777
3	13.874346477206565	17.458454727861714	32.15645102431985	36.51074777061187
4	18.93187999633811	20.64689935414901	16.149980952268454	44.27123969724443
5	21.667496106412266	24.273092260255577	19.647124258252507	34.41228737507965
6	17.66187361881584	31.403423254842544	22.466259003558715	28.468444122782905
7	11.891470617269041	34.40473605794895	31.666519408546268	22.037273916235733
8	10.378833592922993	37.24514554751357	26.323013339569705	26.05300751999373
9	10.52381396699013	43.70247277811348	27.37863452173604	18.395078733160343
10-11	10.84706631593377	39.6235092083312	30.169433116271705	19.359991359463326
12-13	10.266558193611658	43.88401638270669	26.413240767387208	19.43618465629444
14-15	10.843243676804763	40.01739687636075	25.935608428841455	23.20375101799303
16-17	10.929983495146892	42.94570194382047	26.00272034386748	20.12159421716516
18-19	14.598813484856437	34.77842092115251	26.879992611403402	23.742772982587653
20-21	17.93677698639999	35.006434003453116	26.83086503251329	20.225923977633602
22-23	10.146550767622475	39.043037479440876	26.51807799612947	24.29233375680718
24-25	10.224611995001768	38.75095311439164	26.250690141424077	24.773744749182516
26-27	10.31222813867265	38.84351294334502	29.842023600290435	21.00223531769189
28-29	10.415378486703819	38.481065237499784	25.86674816507337	25.236808110723025
30-31	10.198374293330538	34.99867390514165	30.050277934002168	24.752673867525647
32-33	10.284101470027107	39.12510672296776	27.617433503727685	22.973358303277454
34-35	10.422031855404764	38.844781032627616	27.421017529269964	23.312169582697656
36-37	10.581876610629038	38.43404794841372	27.567000772777657	23.41707466817959
38-39	10.405313799771205	38.695555026414844	27.70814190222589	23.19098927158806
40-41	10.393246759611484	38.74360363164611	27.50414969025875	23.358999918483654
42-43	10.360193505778414	38.46345706180077	27.67829403696082	23.49805539546
44-45	10.435190552168567	38.47484446329483	27.719408493713683	23.370556490822917
46-47	10.496315415544533	38.25710439545567	27.63548412624607	23.61109606275373
48-49	10.408692307884317	38.372331247927505	27.72429633154055	23.49468011264763
50-51	10.395617770491851	38.44832931024912	27.685724126844207	23.470328792414826
52-53	10.426961880722104	38.50677725858383	27.702297574902275	23.36396328579179
54-55	10.318496618147579	38.46314523847267	27.845942585404828	23.372415557974925
56-57	10.29991940030278	38.34929081834792	27.85543350167139	23.495356279677914
58-59	10.36783165677678	38.50770524465854	27.800901596731865	23.323561501832817
60-61	10.281979446018731	38.57067029223509	27.84199643303901	23.305353828707165
62-63	10.290579558694212	38.67655416198632	27.79028554527887	23.24258073404059
64-65	10.3154544861964	38.53854799237146	27.731039383691762	23.414958137740374
66-67	10.278246864281147	38.46052018043588	27.96019424674283	23.301038708540144
68-69	10.296666516923436	38.58617080189885	27.95712201992751	23.160040661250203
70-71	10.295228933024005	38.44685560165203	27.97763586936882	23.280279595955143
72-73	10.273247386250874	38.39413563980199	28.062031267924027	23.270585706023112
74-75	10.249347812947065	38.57225984966059	28.044335614769945	23.13405672262241
76-77	10.306854619478141	38.44913457543096	27.98222601811243	23.261784786978467
78-79	10.2901617524676	38.46813453345136	28.032776689177545	23.20892702490349
80-81	10.336952275310377	38.57486650828103	28.055994763476384	23.032186452932216
82-83	10.316009049187267	38.446414923930675	27.938413614903805	23.29916241197825
84-85	10.271908290042612	38.38607243007613	28.090644476246098	23.251374803635155
86-87	10.350230810375285	38.709622813343216	28.008868194262153	22.93127818201934
88-89	10.47572491397492	38.57947311751398	27.892684746462614	23.052117222048484
90-91	10.523788042271507	38.47618464946605	27.901861594572786	23.09816571368966
92-93	10.928042910410795	38.46789257982977	27.75426907106485	22.849795438694574
94-95	11.031407305836243	38.81334659261563	27.451358634223173	22.70388746732496
96-97	11.280380838515331	38.94609920278684	27.26765865010205	22.505861308595783
98-99	11.457796243812018	38.863230759744795	27.033523872106613	22.645449124336576
100-101	11.575138571636751	39.00745047501496	26.614331037413024	22.803079915935257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8076.0
1	7952.5
2	7246.0
3	5874.0
4	5102.0
5	5207.0
6	5442.0
7	5716.0
8	6012.0
9	6602.0
10	8172.5
11	10495.5
12	13350.0
13	18756.0
14	26417.0
15	34888.0
16	45374.5
17	58832.5
18	75504.0
19	96001.0
20	121592.0
21	154222.5
22	195742.5
23	247502.0
24	307380.0
25	372426.0
26	441520.0
27	512464.5
28	578655.5
29	635837.5
30	682195.0
31	714572.5
32	733422.5
33	736386.0
34	723034.5
35	700357.0
36	668888.5
37	629389.0
38	587962.0
39	543479.0
40	497343.5
41	454113.5
42	412754.5
43	370998.5
44	332471.0
45	299362.5
46	268893.5
47	240710.5
48	213799.5
49	190351.5
50	172294.0
51	154578.0
52	136005.5
53	120368.0
54	107729.0
55	97133.0
56	88902.5
57	82367.5
58	76970.0
59	72226.0
60	67851.0
61	63568.0
62	59829.5
63	55587.5
64	51204.5
65	47853.5
66	44136.0
67	40555.5
68	37399.5
69	34239.0
70	30878.0
71	27909.5
72	25538.0
73	23404.0
74	21659.5
75	19862.5
76	18375.0
77	17468.0
78	16746.0
79	15733.0
80	14796.5
81	14178.0
82	13651.0
83	12885.5
84	12178.0
85	11979.5
86	11810.5
87	11703.0
88	10194.5
89	8678.0
90	7875.5
91	7093.0
92	6352.5
93	5343.0
94	4872.5
95	4627.5
96	4472.5
97	4357.5
98	4308.5
99	4295.5
100	4296.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0023023395385565497
3	0.00834732981023368
4	0.008209046014360516
5	4.4341696522177276E-4
6	3.697824436762173E-5
7	5.919920933536011E-5
8	1.8507712756152297E-4
9	4.443178632220776E-5
10-11	0.0
12-13	1.1115559805157575E-5
14-15	2.594556479658325E-5
16-17	1.1123116661694634E-5
18-19	1.2240129985729493E-4
20-21	7.050957716742544E-5
22-23	7.018238843144701E-4
24-25	7.062166579783433E-5
26-27	4.095486948371882E-5
28-29	3.730687072430618E-4
30-31	5.384368572293168E-4
32-33	1.8744668079164882E-4
34-35	6.50397627920901E-4
36-37	1.0558090879973889E-4
38-39	7.185752573889297E-5
40-41	3.793220763438025E-5
42-43	0.0028688909601892677
44-45	0.0011068883109998896
46-47	0.0030129838547709622
48-49	0.0027034085488920846
50-51	0.0033552933624284654
52-53	7.728655559403568E-6
54-55	0.0
56-57	0.0
58-59	4.2929070794018436E-5
60-61	0.0
62-63	1.1395094325447352E-4
64-65	3.943373161402263E-6
66-67	5.185776689213315E-4
68-69	0.0
70-71	2.0364237141301932E-4
72-73	8.021940971189119E-6
74-75	0.0
76-77	0.001062715377321151
78-79	6.117637270603896E-5
80-81	0.0
82-83	1.9000101485324672E-4
84-85	0.0
86-87	0.0
88-89	9.141037657049736E-4
90-91	3.786968704069855E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	20165.0
5-9	30884.0
10-14	11753.0
15-19	13387.0
20-24	27696.0
25-29	67611.0
30-34	90188.0
35-39	98729.0
40-44	101144.0
45-49	99866.0
50-54	102904.0
55-59	107564.0
60-64	112131.0
65-69	131869.0
70-74	153614.0
75-79	176267.0
80-84	216494.0
85-89	95318.0
90-94	131873.0
95-99	277089.0
100-102	1.1484901E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.46476519425663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50805319962973	75.54233408496384
2	6.664451550821396	11.124937676756145
3	1.306848167594038	3.2722732635834277
4	0.5987333718570415	1.9989256118405403
5	0.26651511284539464	1.1122310657180838
6	0.17972621847074163	0.9000483974347273
7	0.12413335216522475	0.7252532763872517
8	0.06743717521021776	0.4502902395427816
9	0.05663065414906515	0.42539978262139905
>10	0.21362079441088647	2.9377564806174594
>50	0.008460484030226305	0.497226923046803
>100	0.00504977726800443	0.7864086453903142
>500	2.776169824188589E-4	0.15091172805424719
>1k	6.252456556419904E-5	0.07600282404290395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.4020642961596648E-4	0.0	2.213785730778418E-5
2	1.4020642961596648E-4	0.0	2.213785730778418E-5
3	1.6234428692375066E-4	0.0	2.213785730778418E-5
4	1.6234428692375066E-4	0.0	2.213785730778418E-5
5	1.6234428692375066E-4	0.0	2.213785730778418E-5
6	1.6234428692375066E-4	0.0	2.213785730778418E-5
7	1.6972357269301203E-4	0.0	2.213785730778418E-5
8	1.6972357269301203E-4	0.0	2.213785730778418E-5
9	1.6972357269301203E-4	0.0	3.689642884630696E-5
10-11	1.8817178711616554E-4	0.0	4.058607173093766E-5
12-13	1.955510728854269E-4	0.0	4.427571461556836E-5
14-15	2.103096444239497E-4	0.0	5.1655000384829754E-5
16-17	2.213785730778418E-4	0.0	5.1655000384829754E-5
18-19	2.361371446163646E-4	0.0	5.1655000384829754E-5
20-21	2.4351643038562597E-4	7.379285769261394E-6	5.1655000384829754E-5
22-23	2.6196464480877945E-4	7.379285769261394E-6	5.534464326946045E-5
24-25	2.951714307704557E-4	7.379285769261394E-6	6.641357192335254E-5
26-27	3.099300023089785E-4	1.4758571538522787E-5	6.641357192335254E-5
28-29	3.209989309628706E-4	2.213785730778418E-5	6.641357192335254E-5
30-31	3.4313678827065477E-4	2.5827500192414877E-5	6.641357192335254E-5
32-33	3.689642884630697E-4	2.9517143077045575E-5	6.641357192335254E-5
34-35	3.874125028862231E-4	2.9517143077045575E-5	6.641357192335254E-5
36-37	3.911021457708538E-4	2.9517143077045575E-5	6.641357192335254E-5
38-39	4.0586071730937663E-4	2.9517143077045575E-5	6.641357192335254E-5
40-41	4.316882175017915E-4	2.9517143077045575E-5	6.641357192335254E-5
42-43	4.98101789425144E-4	2.9517143077045575E-5	8.117214346187533E-5
44-45	5.497567898099738E-4	2.9517143077045575E-5	8.117214346187533E-5
46-47	5.829635757716501E-4	3.689642884630696E-5	8.117214346187533E-5
48-49	6.346185761564798E-4	5.1655000384829754E-5	8.117214346187533E-5
50-51	6.715150050027867E-4	5.1655000384829754E-5	8.117214346187533E-5
52-53	7.637560771185541E-4	5.1655000384829754E-5	9.224107211576742E-5
54-55	8.559971492343216E-4	5.1655000384829754E-5	9.59307150003981E-5
56-57	9.408589355808276E-4	5.1655000384829754E-5	9.59307150003981E-5
58-59	0.0010404792934658564	5.1655000384829754E-5	9.59307150003981E-5
60-61	0.0011659271515433002	5.1655000384829754E-5	9.59307150003981E-5
62-63	0.0012913750096207439	5.903428615409115E-5	1.0331000076965951E-4
64-65	0.0014242021534674489	5.903428615409115E-5	1.0331000076965951E-4
66-67	0.00153489144000637	5.903428615409115E-5	1.0331000076965951E-4
68-69	0.0017415114415456887	5.903428615409115E-5	1.0331000076965951E-4
70-71	0.002143682515970435	5.903428615409115E-5	1.0331000076965951E-4
72-73	0.002900059307319728	5.903428615409115E-5	1.069996436542902E-4
74-75	0.004213572174248255	5.903428615409115E-5	1.106892865389209E-4
76-77	0.005744773971369995	5.903428615409115E-5	1.106892865389209E-4
78-79	0.007766698272147617	5.903428615409115E-5	1.106892865389209E-4
80-81	0.01006165614638791	5.903428615409115E-5	1.180685723081823E-4
82-83	0.012699750808898857	5.903428615409115E-5	1.180685723081823E-4
84-85	0.015931877975835347	5.903428615409115E-5	1.180685723081823E-4
86-87	0.38863746432392055	6.272392903872184E-5	1.180685723081823E-4
88-89	0.9379072212731232	6.641357192335254E-5	1.2175821519281299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	156165	0.0	59.555622	5
ATCATAA	164135	0.0	57.54125	6
CATAAAC	163930	0.0	57.10777	8
TCATAAA	165110	0.0	56.809017	7
TAAATCA	148375	0.0	56.72206	3
AAATCAT	163910	0.0	55.450924	4
ATAAATC	154405	0.0	54.564117	2
ATAAACA	210280	0.0	44.660683	9
AATAAAT	197845	0.0	42.451103	1
AGAGCAC	9210	0.0	38.626896	94-95
AAACGGG	134800	0.0	35.522057	14-15
ACGGGCA	136095	0.0	35.046062	16-17
GGGCAAC	136650	0.0	34.480618	18-19
CAAACGG	135170	0.0	34.436584	14-15
AACGGGC	135190	0.0	33.735058	16-17
CGGGCAA	136325	0.0	33.26855	18-19
GCAACAT	143165	0.0	33.045364	20-21
ACAAACG	149765	0.0	32.50408	12-13
GGCAACA	141065	0.0	31.925486	20-21
CATACCT	154295	0.0	30.049639	24-25
>>END_MODULE