##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 20150414_trimmed_2112_lane1_ATCACG_L001_R1_003.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173081 Sequences flagged as poor quality 0 Sequence length 1-101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.556308558403042 28.0 16.0 31.0 10.0 31.0 2 27.47594707948307 30.0 25.0 31.0 16.0 33.0 3 24.093432231387343 27.0 16.0 31.0 10.0 31.0 4 29.33656186309874 32.0 26.0 35.0 19.0 37.0 5 31.54931227046851 35.0 30.0 35.0 19.0 37.0 6 33.52623789802709 35.0 33.0 35.0 28.0 37.0 7 34.36661230733954 35.0 35.0 37.0 31.0 37.0 8 34.56235288677987 35.0 35.0 37.0 31.0 37.0 9 36.243758565163766 37.0 35.0 39.0 32.0 39.0 10-11 36.52690234401676 38.0 35.0 39.0 32.0 39.0 12-13 36.48395180222523 38.0 35.0 39.0 32.0 39.0 14-15 37.59020475167169 39.0 36.0 40.0 32.0 41.0 16-17 37.56271010618363 39.0 36.0 40.0 32.0 41.0 18-19 37.72689931440378 39.5 37.0 40.0 32.0 41.0 20-21 37.48121851493859 39.0 36.0 40.0 32.0 41.0 22-23 37.62616270363915 39.0 37.0 40.0 32.0 41.0 24-25 37.593288521712196 39.0 37.0 40.0 32.0 41.0 26-27 37.38769279414152 39.0 36.0 40.0 32.0 41.0 28-29 37.25413294732422 39.0 36.0 40.0 31.5 41.0 30-31 37.34401648815748 39.0 36.0 40.0 31.5 41.0 32-33 37.21424512835212 39.0 36.0 40.0 31.0 41.0 34-35 37.13226928463493 39.0 36.0 40.0 31.0 41.0 36-37 37.0595826217688 39.0 36.0 40.0 30.5 41.0 38-39 37.1199134201834 39.0 36.0 40.0 31.0 41.0 40-41 37.16280232236769 39.0 36.0 40.0 31.0 41.0 42-43 37.09238632292087 39.0 36.0 40.0 31.0 41.0 44-45 36.989531193876076 39.0 35.0 40.0 31.0 41.0 46-47 37.032201819887874 39.0 35.5 40.0 31.0 41.0 48-49 36.951956063672 39.0 35.0 40.0 31.0 41.0 50-51 36.86382996988426 39.0 35.0 40.0 31.0 41.0 52-53 36.69316730653992 38.0 35.0 40.0 30.5 41.0 54-55 36.614450349630616 38.0 35.0 40.0 30.5 41.0 56-57 36.41865652033169 38.0 34.5 40.0 30.0 41.0 58-59 36.19649013600408 38.0 34.0 40.0 30.0 41.0 60-61 36.10066601531564 37.0 34.0 40.0 30.0 41.0 62-63 35.671519577749336 37.0 34.0 40.0 29.0 41.0 64-65 35.764032344654495 37.0 34.0 39.0 30.0 41.0 66-67 35.568012668001444 36.0 34.0 39.0 30.0 40.5 68-69 35.41184617129029 36.0 34.0 39.0 30.0 40.0 70-71 35.2339059335039 35.5 34.0 39.0 30.0 40.0 72-73 35.070880113894844 35.0 34.0 37.5 30.0 40.0 74-75 34.83661397528785 35.0 34.0 37.0 30.0 39.5 76-77 32.88628287106865 33.5 31.0 35.5 27.5 38.0 78-79 34.18532824279106 35.0 33.0 36.0 30.0 39.0 80-81 34.17557049636793 35.0 33.0 36.0 30.0 37.5 82-83 34.0264306527055 35.0 33.0 36.0 30.0 37.0 84-85 33.85409462259162 35.0 33.0 35.0 30.0 37.0 86-87 33.6977483095258 35.0 33.0 35.0 30.0 36.0 88-89 33.58380400374709 35.0 33.0 35.0 30.0 36.0 90-91 33.49560273139573 35.0 33.0 35.0 30.0 36.0 92-93 33.45580157287597 35.0 33.0 35.0 30.0 36.0 94-95 33.40004023440001 34.0 33.0 35.0 30.0 36.0 96-97 33.400041316028634 34.0 33.0 35.0 30.0 35.5 98-99 33.43030882786232 34.0 33.0 35.0 30.0 35.0 100-101 32.86902043373808 34.0 32.0 35.0 28.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2314 1 0.09364965752773102 2314 2 1.2006096554307675 2314 3 -1.702275444839131 2314 4 -2.3206026435462093 2314 5 0.19821158811345185 2314 6 0.13391105901983025 2314 7 0.16251819337552575 2314 8 0.2669630795274003 2314 9 0.39496945548015816 2314 10-11 0.31388434100196605 2314 12-13 0.3694221427986406 2314 14-15 0.333850519561274 2314 16-17 0.3280336083331079 2314 18-19 0.30640765493066624 2314 20-21 0.4660041709278886 2314 22-23 0.38214212870136066 2314 24-25 0.34882784339865935 2314 26-27 0.33386191866298986 2314 28-29 0.4285325513894662 2314 30-31 0.4685059709419477 2314 32-33 0.3794386294945511 2314 34-35 0.39196418574372416 2314 36-37 0.40876799047284607 2314 38-39 0.3243404818732003 2314 40-41 0.4088676651620631 2314 42-43 0.36584454288178136 2314 44-45 0.39361967868923387 2314 46-47 0.43208900651690385 2314 48-49 0.37665744904793996 2314 50-51 0.37257843234818466 2314 52-53 0.4676017181910055 2314 54-55 0.3371011561031523 2314 56-57 0.3117737301282304 2314 58-59 0.35108558784655486 2314 60-61 0.28794540459384166 2314 62-63 0.3573133962257202 2314 64-65 0.2644701353442471 2314 66-67 0.2874039621369704 2314 68-69 0.16810420418116223 2314 70-71 0.11622974918987694 2314 72-73 0.013056284467452883 2314 74-75 0.021962134580363113 2314 76-77 -0.008547561444373741 2314 78-79 0.10844835654287266 2314 80-81 0.10604845446741251 2314 82-83 0.06417692165465638 2314 84-85 0.09900517671788123 2314 86-87 0.13768846626197728 2314 88-89 0.11601572888143608 2314 90-91 0.14101037832455177 2314 92-93 0.08796161765180699 2314 94-95 0.09114226441525375 2314 96-97 0.13126783242092444 2314 98-99 0.13942945126238726 2314 100-101 0.10378254681953081 2315 1 2.3294189597321555 2315 2 2.0421650515540897 2315 3 2.4355827019549636 2315 4 0.8953880652346307 2315 5 1.627266443741398 2315 6 0.7548317449522912 2315 7 0.4474228949466976 2315 8 0.2892439927894159 2315 9 0.3233353145939404 2315 10-11 0.24496940790743338 2315 12-13 0.2400649749882291 2315 14-15 0.19304503894967695 2315 16-17 0.27248016114579343 2315 18-19 0.2562780185716065 2315 20-21 0.33602414858143703 2315 22-23 0.3153430216928186 2315 24-25 0.21152522678239194 2315 26-27 0.26401020376765416 2315 28-29 0.3284452997452405 2315 30-31 0.27355743866168325 2315 32-33 0.19575318555521193 2315 34-35 0.24609937676832772 2315 36-37 0.28315579135230706 2315 38-39 0.21852105413403677 2315 40-41 0.29415567090291006 2315 42-43 0.15257667929616758 2315 44-45 0.2779351633971956 2315 46-47 0.15587844320647548 2315 48-49 0.21760592187418837 2315 50-51 0.17677147165384355 2315 52-53 0.1676319010162075 2315 54-55 0.15304610832120602 2315 56-57 0.15582492251414237 2315 58-59 0.22032215544420097 2315 60-61 0.1655825644943434 2315 62-63 0.20930679102711025 2315 64-65 0.18900161329838738 2315 66-67 0.16530486108184306 2315 68-69 0.11790391382783127 2315 70-71 0.12274389676530717 2315 72-73 0.09858724891714132 2315 74-75 0.10746241527020572 2315 76-77 -0.009422423355765375 2315 78-79 0.05808758395896518 2315 80-81 0.10679268648968332 2315 82-83 0.10830144032940581 2315 84-85 0.13220619492535235 2315 86-87 0.066280253884905 2315 88-89 0.12515861274854956 2315 90-91 0.12001433311626641 2315 92-93 0.11207820248514366 2315 94-95 0.10957906864930322 2315 96-97 0.09354237203760363 2315 98-99 0.06687089030772597 2315 100-101 0.030450693386399053 2312 1 -1.8904894326101918 2312 2 -0.2299618000734931 2312 3 -2.2741161813000303 2312 4 -2.381812134692005 2312 5 -0.11595734320914275 2312 6 -0.10487754566791807 2312 7 -0.017001748201465716 2312 8 0.05763952366620373 2312 9 0.10271870368742242 2312 10-11 0.06322156611456364 2312 12-13 0.1427914960160379 2312 14-15 0.1478728618720737 2312 16-17 0.14076085907827007 2312 18-19 0.037857232452147116 2312 20-21 0.1473947925915695 2312 22-23 -0.005986715854390923 2312 24-25 0.08740077374402233 2312 26-27 0.033648667406232846 2312 28-29 0.12815510199448 2312 30-31 0.09105308935916412 2312 32-33 0.028853266805320743 2312 34-35 0.04220778155714555 2312 36-37 0.0974456540012767 2312 38-39 0.09652537197229805 2312 40-41 -0.004279599259348288 2312 42-43 0.03097771029593588 2312 44-45 -0.023342677050223415 2312 46-47 0.05408935626297762 2312 48-49 -0.004134065054493874 2312 50-51 0.04253918622298869 2312 52-53 0.06248915661261378 2312 54-55 0.027972363474852102 2312 56-57 0.08992046269025877 2312 58-59 -0.015625426171496315 2312 60-61 -0.045403909126321196 2312 62-63 0.07055468648392349 2312 64-65 0.03933104347661498 2312 66-67 0.07618682482834771 2312 68-69 0.04768364659550173 2312 70-71 -0.03379881981935995 2312 72-73 -0.07074667317252192 2312 74-75 -0.08231358748846418 2312 76-77 0.05748107202617092 2312 78-79 -0.05882663777337882 2312 80-81 -0.08453880983407203 2312 82-83 -0.10154321750672324 2312 84-85 -0.11456554077098247 2312 86-87 -0.061761675960575246 2312 88-89 -0.03971925730009929 2312 90-91 0.016374919071616034 2312 92-93 -0.0058632247574408325 2312 94-95 0.032172947677267416 2312 96-97 -0.007562633233142435 2312 98-99 0.01880089008874819 2312 100-101 -0.01174513584577852 2313 1 0.0694199961326305 2313 2 0.7241310782789654 2313 3 -0.5389348183120646 2313 4 0.12872014836867507 2313 5 1.2790969403031411 2313 6 0.4211537795036122 2313 7 0.21040775497201736 2313 8 -0.0587584499792797 2313 9 -0.02193905233399107 2313 10-11 0.048544591025901696 2313 12-13 -0.22753279354216716 2313 14-15 -0.23647745101413165 2313 16-17 -0.08736039539283524 2313 18-19 -0.12111772268100651 2313 20-21 -0.11002297439885922 2313 22-23 -0.1150641495743514 2313 24-25 -0.1363388968993604 2313 26-27 -0.08097311033760946 2313 28-29 -0.08703188342337853 2313 30-31 -0.03348527337274021 2313 32-33 -0.07621453005150158 2313 34-35 -0.06510891780451544 2313 36-37 -0.016577517993667357 2313 38-39 -0.07176357499366048 2313 40-41 -0.03746312706616095 2313 42-43 -0.004179731690555855 2313 44-45 -0.03632541444665094 2313 46-47 -0.053916186880407224 2313 48-49 -0.055485824728975786 2313 50-51 -0.08851877000381592 2313 52-53 0.00985738154386695 2313 54-55 0.02971968170609074 2313 56-57 0.0066151322669938395 2313 58-59 -0.016151990276803474 2313 60-61 -0.029908760505414023 2313 62-63 0.03769445943742511 2313 64-65 0.010266235761029918 2313 66-67 -0.07680395685849106 2313 68-69 -0.05590986358522798 2313 70-71 -0.08186763951276532 2313 72-73 -0.055598433053233975 2313 74-75 -0.05075116807112323 2313 76-77 -0.05386894937350206 2313 78-79 -0.02494053096251747 2313 80-81 0.0265563357797447 2313 82-83 0.056233023969760154 2313 84-85 0.009563870621022375 2313 86-87 0.008143216273637677 2313 88-89 -0.032573923723845155 2313 90-91 -0.05685600661431778 2313 92-93 -0.032359466833447925 2313 94-95 0.02055818916601737 2313 96-97 -0.04164781722403177 2313 98-99 -0.06772362830685097 2313 100-101 0.04350161795240126 2316 1 -0.6019991807823182 2316 2 -3.7369439851903508 2316 3 2.079743742496259 2316 4 3.678306564634891 2316 5 -2.988617628948859 2316 6 -1.2050190378078014 2316 7 -0.8033470950927466 2316 8 -0.555088146003726 2316 9 -0.7990844214275583 2316 10-11 -0.670619906049879 2316 12-13 -0.5247458202607405 2316 14-15 -0.4382909693688788 2316 16-17 -0.6539142331643717 2316 18-19 -0.47942518327342043 2316 20-21 -0.839400137702043 2316 22-23 -0.5764342849654582 2316 24-25 -0.5114149470257132 2316 26-27 -0.550547679499239 2316 28-29 -0.798101069705794 2316 30-31 -0.7996312255900193 2316 32-33 -0.5278305518035822 2316 34-35 -0.615162426264682 2316 36-37 -0.7727919178327411 2316 38-39 -0.567623332985896 2316 40-41 -0.6612806097394852 2316 42-43 -0.5452192007833148 2316 44-45 -0.6118867505895622 2316 46-47 -0.5881406191059426 2316 48-49 -0.5346434811387155 2316 50-51 -0.5033703202211939 2316 52-53 -0.7075801573636866 2316 54-55 -0.5478393096053082 2316 56-57 -0.5641342475996396 2316 58-59 -0.5396303268424774 2316 60-61 -0.37821529945647114 2316 62-63 -0.6748693331742004 2316 64-65 -0.5030690278802794 2316 66-67 -0.452091691188663 2316 68-69 -0.27778190101927436 2316 70-71 -0.12330718662307305 2316 72-73 0.014701572841147481 2316 74-75 0.003640205708990152 2316 76-77 0.014357862147463152 2316 78-79 -0.08276877176592734 2316 80-81 -0.1548586669027614 2316 82-83 -0.1271681684471062 2316 84-85 -0.1262097014932806 2316 86-87 -0.1503502604599447 2316 88-89 -0.1688811606060625 2316 90-91 -0.22054362389813775 2316 92-93 -0.16181712854604058 2316 94-95 -0.25345246990784176 2316 96-97 -0.17559975400135386 2316 98-99 -0.15737760335200335 2316 100-101 -0.16598972231253128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 120.0 15 1330.0 16 1142.0 17 971.0 18 581.0 19 502.0 20 560.0 21 442.0 22 597.0 23 895.0 24 1751.0 25 3693.0 26 7246.0 27 13528.0 28 21686.0 29 31759.0 30 43328.0 31 55057.0 32 68942.0 33 85915.0 34 110262.0 35 145495.0 36 197457.0 37 230970.0 38 133541.0 39 15237.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.123832881105397 31.364074603544 16.309274466128084 38.20281804922252 2 18.117085437844977 28.032393495727042 25.684973254054515 28.165547812373465 3 13.795367269403652 16.156409224536013 34.707708940105995 35.34051456595434 4 19.856222238900678 22.227045707306754 13.729590687963539 44.18714136582903 5 20.012574866157124 25.178179531842517 21.58605625031501 33.22318935168535 6 17.398028801926056 32.28905547328931 22.148961564373433 28.163954160411205 7 11.749400649916236 34.335872193407404 32.28944031568338 21.62528684099298 8 10.207870070288973 37.15468797227143 25.969844840237933 26.667597117201662 9 10.30757503083459 44.53080032890229 27.13760792106345 18.024016719199672 10-11 10.652630640943475 40.22065098552642 30.39164864466554 18.735069728864563 12-13 10.088812961326374 44.70953850004586 26.130991617585668 19.070656921042097 14-15 10.662632185775925 40.3875862961999 25.6368907169922 23.312890801031973 16-17 10.73715357119418 43.81921980944995 25.713361624295285 19.730264995060594 18-19 14.960643776068858 34.65888339991769 26.513962543672516 23.866510280340943 20-21 18.762647084084858 34.88937559048355 26.495705574164734 19.852271751266855 22-23 9.960587064082228 39.453762751401975 26.27288397744468 24.31276620707112 24-25 10.054705555393031 39.14019868837451 25.9865154849934 24.818580271239057 26-27 10.152593845387315 39.16611282221032 30.048841753924105 20.63245157847825 28-29 10.172658644143807 38.872627147971954 25.61433736850132 25.340376839382916 30-31 10.012173619203994 34.94963760738562 30.246894860653217 24.791293912757173 32-33 10.144376453900833 39.44893328819622 27.61035088306346 22.79633937483948 34-35 10.303622966843593 39.17399688312232 27.32763723137866 23.194742918655425 36-37 10.286848563086014 38.810077227448765 27.596026350867298 23.307047858597926 38-39 10.29506994607156 39.031626852292696 27.694960304323992 22.978342897311755 40-41 10.27934903427847 39.03588024708845 27.455826475035888 23.228944243597194 42-43 10.301229313960071 38.75687541469525 27.621250468400778 23.320644802943903 44-45 10.320409716036762 38.773520006652504 27.667616664524765 23.23845361278597 46-47 10.397505880462582 38.566863202810126 27.535427834338332 23.500203082388964 48-49 10.327503191274943 38.67108592927686 27.69623541531364 23.305175464134546 50-51 10.320451110848323 38.7758478899255 27.598390482823675 23.305310516402496 52-53 10.372103225899634 38.78508806770995 27.65241238364158 23.19039632274884 54-55 10.258856005626082 38.68611749523305 27.857629650037335 23.197396849103523 56-57 10.235811372739564 38.5930934455794 27.874394684690095 23.296700496990937 58-59 10.32551948923933 38.77517742611774 27.723007889969768 23.176295194673152 60-61 10.259469761239394 38.78202100447659 27.83615921286866 23.122350021415357 62-63 10.256547219690862 38.85226282172011 27.770167066747035 23.121022891841996 64-65 10.296493713926443 38.71403046070135 27.734216627410007 23.255259197962197 66-67 10.249368559386983 38.67661374217169 27.892011933891038 23.18200576455029 68-69 10.259316884543065 38.72949921832977 27.98878161056695 23.02240228656021 70-71 10.247104416954599 38.64115736275318 27.951192186032408 23.160546034259816 72-73 10.283939817149959 38.58799605324453 28.01923737741014 23.108826752195373 74-75 10.220663156248863 38.7057338031316 28.049960863266126 23.023642177353413 76-77 10.380452233522888 38.54748337991454 27.94900830481778 23.123056081744792 78-79 10.302599955781604 38.576683343273714 28.081738136361707 23.038978564582973 80-81 10.301548210390605 38.70593225264588 28.08591316360709 22.90660637335643 82-83 10.356017256402247 38.59239051071949 27.886970758695657 23.16462147418261 84-85 10.329397976645053 38.48042331753638 28.111277215409995 23.078901490408573 86-87 10.388123275167995 38.799267463052765 27.97468403963604 22.8379252221432 88-89 10.556985316666871 38.62783822573325 27.93179671495688 22.883379742643 90-91 10.634239084416212 38.52640357285622 27.862331611625173 22.977025731102398 92-93 11.015640208984102 38.507060180267075 27.80122002919137 22.67607958155746 94-95 11.162848870046684 38.79962994984272 27.481733240291394 22.5557879398192 96-97 11.418376672218477 38.94056103388434 27.243030000234587 22.398032293662602 98-99 11.596112476178403 38.90215385482258 27.038696136021073 22.463037532977946 100-101 11.78605202872912 39.065824657802274 26.58370902391104 22.56441428955757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 658.0 1 657.5 2 623.0 3 549.0 4 513.5 5 532.0 6 562.5 7 589.0 8 605.0 9 655.0 10 813.0 11 1035.0 12 1281.0 13 1900.0 14 2747.0 15 3575.0 16 4618.0 17 5866.0 18 7339.0 19 9246.5 20 11625.0 21 14530.5 22 18365.5 23 23327.5 24 28726.5 25 34505.0 26 40993.0 27 47433.0 28 53115.0 29 57941.5 30 62011.5 31 64750.5 32 66148.0 33 66272.5 34 64663.5 35 62260.0 36 59116.0 37 55382.0 38 51530.5 39 47453.0 40 43391.0 41 39455.5 42 35590.0 43 31820.5 44 28515.5 45 25669.0 46 22966.0 47 20412.5 48 18293.0 49 16371.0 50 14686.0 51 13190.0 52 11811.0 53 10572.5 54 9402.0 55 8546.0 56 7897.0 57 7227.5 58 6702.5 59 6385.5 60 6119.5 61 5794.5 62 5439.5 63 5019.0 64 4551.5 65 4174.5 66 3897.5 67 3681.0 68 3388.5 69 3104.5 70 2840.0 71 2577.5 72 2396.0 73 2212.5 74 2094.5 75 1954.5 76 1818.5 77 1758.0 78 1696.0 79 1564.0 80 1448.0 81 1399.5 82 1359.5 83 1308.5 84 1254.5 85 1237.0 86 1217.0 87 1206.0 88 978.5 89 748.0 90 681.0 91 618.5 92 554.0 93 458.5 94 410.5 95 389.0 96 374.5 97 364.5 98 362.5 99 362.0 100 362.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.2622839022061576E-4 3 0.004518617984952149 4 0.014155730086042961 5 1.7085386783257346E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.28415057075596E-5 12-13 0.0 14-15 0.0 16-17 4.28954055589014E-5 18-19 0.0012445518669136316 20-21 0.0 22-23 0.0 24-25 4.302102222250902E-5 26-27 0.0 28-29 4.3204606647979235E-5 30-31 0.0 32-33 9.994959064124182E-4 34-35 4.797857538524615E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.858635663804145E-5 44-45 0.002089981252423489 46-47 0.0057127606112296805 48-49 0.003314337105705855 50-51 0.007553314938613579 52-53 0.0 54-55 4.5313147836410477E-5 56-57 0.0 58-59 0.0 60-61 3.6685703489635827E-4 62-63 2.3023946746532134E-4 64-65 1.8491816677677003E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0011399757185171955 78-79 1.9100782893338838E-4 80-81 0.0 82-83 6.769446201276911E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 3.458680868998509E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 2490.0 5-9 3383.0 10-14 1231.0 15-19 1490.0 20-24 2667.0 25-29 6857.0 30-34 9731.0 35-39 11023.0 40-44 10927.0 45-49 10041.0 50-54 10899.0 55-59 10825.0 60-64 11079.0 65-69 10947.0 70-74 12402.0 75-79 14544.0 80-84 18112.0 85-89 7930.0 90-94 10285.0 95-99 20040.0 100-102 986178.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.38114625304509 #Duplication Level Percentage of deduplicated Percentage of total 1 97.87365067448812 94.33174639977285 2 1.6249317829442163 3.132255756463357 3 0.25218772434204906 0.7291842582910094 4 0.0883509741861869 0.34061472658551567 5 0.04990910873717677 0.24051485542784826 6 0.030751048874536578 0.17782928034067486 7 0.024559169320869476 0.16569286231176134 8 0.01161074024788342 0.08952451631498952 9 0.005917675079993768 0.05133170766265918 >10 0.03599802832938232 0.5715070278666908 >50 0.0017039373982879649 0.1092008112413403 >100 4.2913605125551816E-4 0.06059779772122888 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0 20-21 0.0 0.0 0.0 22-23 0.0 0.0 0.0 24-25 8.524560537592886E-5 0.0 0.0 26-27 8.524560537592886E-5 0.0 0.0 28-29 8.524560537592886E-5 4.262280268796443E-5 0.0 30-31 1.7049121075185772E-4 8.524560537592886E-5 0.0 32-33 2.5573681612778656E-4 8.524560537592886E-5 0.0 34-35 2.5573681612778656E-4 8.524560537592886E-5 0.0 36-37 2.98359618815751E-4 8.524560537592886E-5 0.0 38-39 3.4098242150371543E-4 8.524560537592886E-5 0.0 40-41 3.4098242150371543E-4 8.524560537592886E-5 0.0 42-43 3.8360522419167984E-4 8.524560537592886E-5 0.0 44-45 4.262280268796443E-4 8.524560537592886E-5 0.0 46-47 4.688508295676087E-4 8.524560537592886E-5 0.0 48-49 5.114736322555731E-4 8.524560537592886E-5 0.0 50-51 5.540964349435376E-4 8.524560537592886E-5 8.524560537592886E-5 52-53 5.96719237631502E-4 8.524560537592886E-5 8.524560537592886E-5 54-55 5.96719237631502E-4 8.524560537592886E-5 8.524560537592886E-5 56-57 6.393420403194665E-4 8.524560537592886E-5 8.524560537592886E-5 58-59 7.672104483833597E-4 8.524560537592886E-5 1.2786840806389328E-4 60-61 8.950788564472531E-4 8.524560537592886E-5 1.7049121075185772E-4 62-63 0.0011508156725750396 8.524560537592886E-5 1.7049121075185772E-4 64-65 0.0012786840806389328 8.524560537592886E-5 1.7049121075185772E-4 66-67 0.0014491752913907906 8.524560537592886E-5 1.7049121075185772E-4 68-69 0.0014491752913907906 8.524560537592886E-5 1.7049121075185772E-4 70-71 0.0018327805155824704 8.524560537592886E-5 1.7049121075185772E-4 72-73 0.002898350582781581 8.524560537592886E-5 1.7049121075185772E-4 74-75 0.00413441186073255 8.524560537592886E-5 1.7049121075185772E-4 76-77 0.005370473138683518 8.524560537592886E-5 1.7049121075185772E-4 78-79 0.007373744865017846 8.524560537592886E-5 1.7049121075185772E-4 80-81 0.009291770985976245 8.524560537592886E-5 1.7049121075185772E-4 82-83 0.011763893541878182 1.7049121075185772E-4 1.7049121075185772E-4 84-85 0.015472077375731088 1.7049121075185772E-4 1.7049121075185772E-4 86-87 0.3802380227793307 1.7049121075185772E-4 1.7049121075185772E-4 88-89 0.930967256310519 1.7049121075185772E-4 1.7049121075185772E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCATA 14100 0.0 61.4235 5 ATCATAA 14790 0.0 59.126827 6 CATAAAC 14635 0.0 59.099037 8 TCATAAA 14730 0.0 58.733128 7 TAAATCA 13185 0.0 56.3266 3 AAATCAT 14970 0.0 56.18293 4 ATAAATC 13260 0.0 51.27979 2 ATAAACA 18895 0.0 45.999516 9 TACGGCG 115 1.8189894E-12 42.742188 6 AATAAAT 16805 0.0 40.33537 1 AGAGCAC 675 0.0 40.144215 94-95 AGGCCCC 90 2.0765583E-8 39.665623 1 ACCGGGC 105 1.884473E-9 38.268642 2 GCACGTT 160 0.0 36.256855 1 AAACGGG 12080 0.0 36.08177 14-15 ACGGGCA 12190 0.0 35.53498 16-17 GGGCAAC 12255 0.0 35.13457 18-19 ATACGGC 140 2.3646862E-11 35.094612 5 CAAACGG 12225 0.0 34.939632 14-15 CCAACGG 130 4.0017767E-10 34.34365 2 >>END_MODULE