##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_CAGATC_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12742235
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.069716890325754	31.0	28.0	31.0	23.0	34.0
2	30.386023616130405	31.0	30.0	33.0	26.0	34.0
3	27.596537340068576	31.0	26.0	31.0	16.0	34.0
4	32.07169869892747	35.0	32.0	37.0	19.0	37.0
5	33.081010558116795	35.0	32.0	37.0	26.0	37.0
6	34.319773746804124	35.0	35.0	37.0	30.0	37.0
7	34.859798609814305	35.0	35.0	37.0	32.0	37.0
8	35.00227030055339	35.0	35.0	37.0	32.0	37.0
9	36.656407589884154	39.0	35.0	39.0	32.0	39.0
10-11	36.83468139174239	39.0	36.0	39.0	33.0	39.0
12-13	36.76204623458442	39.0	36.0	39.0	32.5	39.0
14-15	37.81968632386455	39.5	37.0	41.0	33.0	41.0
16-17	37.7759788004694	39.0	37.0	41.0	32.5	41.0
18-19	37.88824212412045	40.0	37.0	41.0	33.0	41.0
20-21	37.85462675230612	40.0	37.0	41.0	32.5	41.0
22-23	37.991062186042235	40.0	37.0	41.0	33.0	41.0
24-25	37.85642251465738	40.0	37.0	41.0	32.5	41.0
26-27	37.58901264869826	39.5	37.0	41.0	32.0	41.0
28-29	37.56797941950965	39.0	36.5	41.0	32.0	41.0
30-31	37.62039634490989	39.5	37.0	41.0	32.0	41.0
32-33	37.4710406405695	39.0	36.5	41.0	31.5	41.0
34-35	37.44226133953905	39.0	36.0	41.0	31.0	41.0
36-37	37.41420021809735	39.0	36.0	41.0	31.0	41.0
38-39	37.417208680261325	39.0	36.0	41.0	31.0	41.0
40-41	37.44021126674562	39.0	36.0	40.5	31.5	41.0
42-43	37.28577469287279	39.0	36.0	40.0	31.0	41.0
44-45	37.12820731959937	39.0	36.0	40.0	31.0	41.0
46-47	37.24180606580852	39.0	36.0	40.0	31.0	41.0
48-49	37.14728133126401	39.0	36.0	40.0	31.0	41.0
50-51	36.91431888634189	39.0	35.0	40.0	31.0	41.0
52-53	36.80708893362021	38.5	35.0	40.0	31.0	41.0
54-55	36.684972267047335	38.0	35.0	40.0	31.0	41.0
56-57	36.46444256931555	38.0	35.0	40.0	30.5	41.0
58-59	36.233720053495375	38.0	34.0	40.0	30.0	41.0
60-61	36.01746134736638	37.0	34.0	40.0	30.0	41.0
62-63	35.78895969530846	37.0	34.0	39.5	30.0	41.0
64-65	35.75744308106594	36.5	34.0	39.0	30.0	41.0
66-67	35.52269722793794	36.0	34.0	39.0	30.0	40.0
68-69	35.29148377151329	36.0	34.0	39.0	30.0	40.0
70-71	35.05747181758193	35.0	34.0	38.0	30.0	40.0
72-73	34.7857461772134	35.0	34.0	37.0	30.0	39.5
74-75	34.47508467715981	35.0	33.5	37.0	30.0	39.0
76-77	31.42968671177141	32.5	29.5	34.5	25.5	36.0
78-79	33.73885815357212	34.5	33.0	36.0	29.5	37.0
80-81	33.92223868311437	35.0	33.0	36.0	30.0	37.0
82-83	33.82168436987584	35.0	33.0	35.0	30.0	37.0
84-85	33.69608834261216	35.0	33.0	35.0	30.0	36.0
86-87	33.58269016168575	35.0	33.0	35.0	30.0	36.0
88-89	33.448915829761745	35.0	33.0	35.0	30.0	36.0
90-91	33.38979658832995	35.0	33.0	35.0	30.0	36.0
92-93	33.31887491265138	35.0	33.0	35.0	30.0	35.5
94-95	33.27017655016677	34.5	33.0	35.0	30.0	35.0
96-97	33.27424622872502	34.0	33.0	35.0	30.0	35.0
98-99	33.330824208324486	34.0	33.0	35.0	30.0	35.0
100-101	32.37184761819459	33.5	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1307	1	1.9559295690667255
1307	2	1.204862955633331
1307	3	2.5236120872937455
1307	4	2.1954659439877346
1307	5	2.385737478992233
1307	6	1.1840581693553318
1307	7	0.6267358562184882
1307	8	0.454997696662474
1307	9	0.5298123248087521
1307	10-11	0.4536790062136191
1307	12-13	0.4663088517081633
1307	14-15	0.43809694337001304
1307	16-17	0.48111795369192834
1307	18-19	0.34800067982400407
1307	20-21	0.5375181283151562
1307	22-23	0.33241152992929557
1307	24-25	0.46114396341713615
1307	26-27	0.42589859584018797
1307	28-29	0.5764005950808126
1307	30-31	0.5127613520560956
1307	32-33	0.5103543014245275
1307	34-35	0.5340000384677381
1307	36-37	0.5255388480189112
1307	38-39	0.5276514831223693
1307	40-41	0.520742667948106
1307	42-43	0.43371519969377204
1307	44-45	0.46400927289904814
1307	46-47	0.43939809753391046
1307	48-49	0.45685307211685
1307	50-51	0.4086159661707569
1307	52-53	0.431272722789835
1307	54-55	0.29474589130795437
1307	56-57	0.22518541239697498
1307	58-59	0.21456523008298944
1307	60-61	0.27774928833322576
1307	62-63	0.35837396736300775
1307	64-65	0.2843191042615061
1307	66-67	0.29175232397474815
1307	68-69	0.286968725169892
1307	70-71	0.23211116133986565
1307	72-73	0.19620561627160527
1307	74-75	0.18006776781671618
1307	76-77	-0.5666316750914113
1307	78-79	0.011142839044723019
1307	80-81	0.1414288760603739
1307	82-83	0.10170320175623715
1307	84-85	0.11541135188997487
1307	86-87	0.09517880306535176
1307	88-89	0.0637483362443021
1307	90-91	0.02356261099109247
1307	92-93	0.03462670467582285
1307	94-95	0.05526473882812866
1307	96-97	0.05208984806053962
1307	98-99	0.046105587023021144
1307	100-101	-0.29176600102247363
1306	1	2.187501468206456
1306	2	1.416721993061909
1306	3	3.358488655798343
1306	4	2.60156558229005
1306	5	2.5721338079149874
1306	6	1.3742938346743685
1306	7	0.6837450975290125
1306	8	0.44398706288123435
1306	9	0.5378734209366058
1306	10-11	0.49248101030131863
1306	12-13	0.5020706989441379
1306	14-15	0.42322552531787494
1306	16-17	0.5193704994944781
1306	18-19	0.5091191883357524
1306	20-21	0.7270850355208012
1306	22-23	0.5434015043029845
1306	24-25	0.5263159648425315
1306	26-27	0.5911574071174428
1306	28-29	0.6532618771645389
1306	30-31	0.6421203809409874
1306	32-33	0.6142521081233667
1306	34-35	0.71414788739051
1306	36-37	0.8439746735282228
1306	38-39	0.775421191553832
1306	40-41	0.7332178487491277
1306	42-43	0.5517414419310995
1306	44-45	0.7209573886964122
1306	46-47	0.5670531349819967
1306	48-49	0.6185702796575967
1306	50-51	0.6526130887864525
1306	52-53	0.6860179453994846
1306	54-55	0.5886312197199786
1306	56-57	0.500259602286846
1306	58-59	0.5132829639816876
1306	60-61	0.5027031897102745
1306	62-63	0.5394753242517396
1306	64-65	0.32466184604613346
1306	66-67	0.4245359009916001
1306	68-69	0.4457383307332137
1306	70-71	0.38521497799446536
1306	72-73	0.36173154541807406
1306	74-75	0.30414371000913576
1306	76-77	0.320841189554379
1306	78-79	0.27499846229599
1306	80-81	0.3281882654216588
1306	82-83	0.2783510396740425
1306	84-85	0.3373447005955441
1306	86-87	0.36454166180526926
1306	88-89	0.23035925504773758
1306	90-91	0.24874792174121296
1306	92-93	0.17046822546696916
1306	94-95	0.16974719418217177
1306	96-97	0.2469417108841867
1306	98-99	0.26077140818841116
1306	100-101	0.10709819247187369
1101	1	0.9088334371249864
1101	2	0.45970022251463405
1101	3	1.2166199995495965
1101	4	-1.1371999002028446
1101	5	0.1629074045744474
1101	6	0.16035366336623724
1101	7	0.15550375566821373
1101	8	0.15556732759398528
1101	9	0.2776381558713368
1101	10-11	0.23830826398052807
1101	12-13	0.19883760859342203
1101	14-15	0.2782830131359617
1101	16-17	0.27759443006590345
1101	18-19	0.31055909354380873
1101	20-21	0.33971030384407896
1101	22-23	0.32474444509833944
1101	24-25	0.38113993964586257
1101	26-27	0.3744449785381292
1101	28-29	0.4261670503607249
1101	30-31	0.3898883135450433
1101	32-33	0.3054547321181076
1101	34-35	0.3345834010500326
1101	36-37	0.35419434033472896
1101	38-39	0.31971688343362814
1101	40-41	0.3497513651034936
1101	42-43	0.23909217627893753
1101	44-45	0.24827431563527114
1101	46-47	0.24217700829779432
1101	48-49	0.2640837120479844
1101	50-51	0.20102707642090678
1101	52-53	0.2834178653422228
1101	54-55	0.2954430586859971
1101	56-57	0.27836202337608995
1101	58-59	0.21124583042089995
1101	60-61	0.16769003007976835
1101	62-63	0.2833459371529585
1101	64-65	0.23801355453300488
1101	66-67	0.17686106191833773
1101	68-69	0.18487271305036757
1101	70-71	0.04676060809962479
1101	72-73	-0.018474711897177087
1101	74-75	-0.026022833349038876
1101	76-77	-0.3049360037664961
1101	78-79	-0.06484189650402783
1101	80-81	-0.021118039428586144
1101	82-83	-0.0386519371936771
1101	84-85	-0.11791115583312717
1101	86-87	-0.027260835374207204
1101	88-89	-0.05015896828133748
1101	90-91	0.02865184397977316
1101	92-93	-0.042739171024990696
1101	94-95	-0.15440625305286204
1101	96-97	-0.02776992897175745
1101	98-99	-0.011927831685852652
1101	100-101	-0.16511639746419604
1305	1	0.36266744791179306
1305	2	-0.004586250922216095
1305	3	0.4297527183355392
1305	4	-0.988106972519784
1305	5	0.591852644575404
1305	6	0.27316458250567166
1305	7	0.12887670679750585
1305	8	0.13422321109805324
1305	9	0.10937553175381254
1305	10-11	0.11905819840531251
1305	12-13	0.1542078169261174
1305	14-15	0.15607392890178318
1305	16-17	0.16354861654581754
1305	18-19	0.14333151401415734
1305	20-21	0.23898717163106653
1305	22-23	0.11704822423993733
1305	24-25	0.19278967994475238
1305	26-27	0.15153585991631502
1305	28-29	0.21528163592627436
1305	30-31	0.19855384473036963
1305	32-33	0.13996166738335347
1305	34-35	0.18232103447703452
1305	36-37	0.23295461604579515
1305	38-39	0.21943932806753708
1305	40-41	0.16839535634416336
1305	42-43	0.10791213538313826
1305	44-45	0.11333298605234177
1305	46-47	0.1511103267303966
1305	48-49	0.2557132336005381
1305	50-51	0.18630707635524857
1305	52-53	0.20264446937112268
1305	54-55	0.11719075880148466
1305	56-57	0.11848509777290417
1305	58-59	0.1490941294100523
1305	60-61	0.10665791879742414
1305	62-63	0.11269931182378912
1305	64-65	0.06829119381284698
1305	66-67	0.029392442992858037
1305	68-69	-0.0804230707153124
1305	70-71	-0.030317342282813797
1305	72-73	-0.06541899266255058
1305	74-75	-0.1306794063357657
1305	76-77	-0.33882696855479466
1305	78-79	-0.08337990510912618
1305	80-81	0.030833726190699906
1305	82-83	-0.012069951778002519
1305	84-85	0.020144314101422367
1305	86-87	5.89386462259256E-4
1305	88-89	-0.03217202410870357
1305	90-91	-0.09229783684833137
1305	92-93	-0.032877043654231386
1305	94-95	-0.09517489207078
1305	96-97	-0.05768134673343894
1305	98-99	-0.06004297689829485
1305	100-101	-0.17153299826697577
1102	1	1.8459655172780316
1102	2	0.9689652781617859
1102	3	1.9211623786002043
1102	4	2.8335055388224077
1102	5	2.3398698564850022
1102	6	0.9782936248332561
1102	7	0.4446949515368388
1102	8	0.27180923108765853
1102	9	0.1449968272804938
1102	10-11	0.0651948846377195
1102	12-13	0.04634071498180958
1102	14-15	0.12528485811025547
1102	16-17	0.0848662173240271
1102	18-19	0.13012248541534888
1102	20-21	0.19581736424133567
1102	22-23	0.056667340303853564
1102	24-25	0.03104573043062686
1102	26-27	0.04497262893394094
1102	28-29	0.07124143241406244
1102	30-31	0.16182491785362174
1102	32-33	0.21630325943692696
1102	34-35	0.09429082920726017
1102	36-37	-0.07640405303006048
1102	38-39	-0.026260509072940863
1102	40-41	0.06764439406335043
1102	42-43	-0.030400658386028567
1102	44-45	0.16406197892063545
1102	46-47	0.12134752298688767
1102	48-49	0.06998586074970348
1102	50-51	0.006576338425020367
1102	52-53	0.10982391502249556
1102	54-55	-0.006046956302888873
1102	56-57	0.0676007092740889
1102	58-59	0.01649691067105863
1102	60-61	-0.10324937839153847
1102	62-63	0.07848470058649326
1102	64-65	-0.12789722954541105
1102	66-67	7.293901924256829E-4
1102	68-69	-0.07751852837185425
1102	70-71	-0.07511703779546508
1102	72-73	-0.019501946916243185
1102	74-75	-0.023370724933677423
1102	76-77	-0.2701188315644387
1102	78-79	-0.026195335042686452
1102	80-81	0.008422659274671673
1102	82-83	0.061889932142690895
1102	84-85	0.06221645325500447
1102	86-87	0.029500308610380443
1102	88-89	0.059771715933948144
1102	90-91	0.0374768131989498
1102	92-93	0.06268978299327443
1102	94-95	0.02594158810725844
1102	96-97	-0.0501959543745798
1102	98-99	-0.038611947913395284
1102	100-101	-0.1293407211245281
1304	1	1.5876472098800782
1304	2	0.8269783228678094
1304	3	1.1786205146330033
1304	4	2.59128469937567
1304	5	1.8078297486650001
1304	6	0.7564968382028141
1304	7	0.3769197769827599
1304	8	0.22732649691779017
1304	9	0.09533764182931037
1304	10-11	0.03199211653806344
1304	12-13	0.03346497818859717
1304	14-15	-0.04223122754427777
1304	16-17	-0.0765182681121459
1304	18-19	0.03108089467455244
1304	20-21	0.1237511432697147
1304	22-23	-0.011885101723393632
1304	24-25	0.06517892861868546
1304	26-27	0.005803777156621948
1304	28-29	-0.14159199735158268
1304	30-31	-0.06373541773464808
1304	32-33	-0.004289709287860433
1304	34-35	-0.009857034727808411
1304	36-37	0.026654231830661956
1304	38-39	-0.006813890094150565
1304	40-41	0.07701378642524759
1304	42-43	-0.00600230254619305
1304	44-45	0.008299276374330589
1304	46-47	-0.05379789834989168
1304	48-49	-0.08307773478103542
1304	50-51	-0.09018386379337073
1304	52-53	-0.07655093742955899
1304	54-55	-0.016584567749077905
1304	56-57	-0.07721492329346802
1304	58-59	-0.1769868775395409
1304	60-61	-0.1181024535786861
1304	62-63	-0.14384243948860131
1304	64-65	-0.07195029048183699
1304	66-67	-0.0535853359543097
1304	68-69	-0.046638478277252204
1304	70-71	-0.03324592315675545
1304	72-73	-0.10940122168234723
1304	74-75	-0.03642341693586815
1304	76-77	-0.31966334244143013
1304	78-79	-0.027709730566769508
1304	80-81	0.005009275558549575
1304	82-83	-0.03653347416411634
1304	84-85	-0.039463862879252076
1304	86-87	-0.09355307729020979
1304	88-89	-0.07321541049093838
1304	90-91	0.014245467695197078
1304	92-93	0.024543162313378275
1304	94-95	0.014893556220577864
1304	96-97	-0.022650270725179666
1304	98-99	-0.007517042484835201
1304	100-101	-0.052028051926519936
1103	1	0.853226519419632
1103	2	0.43643322903360016
1103	3	-0.5422042905203561
1103	4	-0.4222170303603363
1103	5	1.1015638891124198
1103	6	0.4329282222980595
1103	7	0.1533396181857256
1103	8	0.01971407827170424
1103	9	-0.02720233170858677
1103	10-11	0.02484286798820534
1103	12-13	-0.11961053953859135
1103	14-15	-0.13770121826813408
1103	16-17	-0.12055206429553778
1103	18-19	-0.0423393717592333
1103	20-21	-0.01357948997525682
1103	22-23	-0.1645163266444385
1103	24-25	-0.08017960019497394
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1212	96-97	0.11032077487423919
1212	98-99	0.0868476156958522
1212	100-101	0.19896840107995928
1213	1	1.0924649741449208
1213	2	0.5021593798214887
1213	3	0.1041582984196161
1213	4	1.7821490001627467
1213	5	0.03544445080758152
1213	6	0.07981941919710778
1213	7	0.07060228622380293
1213	8	0.017898274283794535
1213	9	0.013645176543626292
1213	10-11	-0.029849250540721073
1213	12-13	-0.03144596187949844
1213	14-15	-0.19759426128791802
1213	16-17	-0.07014651857649312
1213	18-19	-0.07281593984234291
1213	20-21	0.019821486681763645
1213	22-23	0.025606093876838543
1213	24-25	-0.022349029691064004
1213	26-27	0.0591269777868888
1213	28-29	-0.04698233417686026
1213	30-31	-0.060037458728118054
1213	32-33	-0.02309377054604056
1213	34-35	-0.12597475387164536
1213	36-37	-0.001722643919627842
1213	38-39	-0.052557908275488785
1213	40-41	-0.02369381355990896
1213	42-43	-0.0065459551864037735
1213	44-45	-0.017706509664385806
1213	46-47	-0.015232701157920303
1213	48-49	-0.09254230625838744
1213	50-51	-0.07980171244326328
1213	52-53	-0.185251562465929
1213	54-55	-0.06895134843480122
1213	56-57	-0.07137269678810299
1213	58-59	-0.18479220252134354
1213	60-61	-0.16333321951709934
1213	62-63	-0.0894380689672829
1213	64-65	0.02371403856902532
1213	66-67	0.03834049749237067
1213	68-69	-0.019418101088746198
1213	70-71	-0.042907698098268554
1213	72-73	0.038810419354540215
1213	74-75	0.026411683400382913
1213	76-77	0.10735778667642748
1213	78-79	0.018619121503789415
1213	80-81	-0.014788163453673064
1213	82-83	-0.0025698687791759767
1213	84-85	0.006086170375091626
1213	86-87	0.04284225823098353
1213	88-89	-0.024736401534610764
1213	90-91	-1.0588047032911163E-5
1213	92-93	0.04419756658315066
1213	94-95	0.03726907388005429
1213	96-97	0.08174330419758746
1213	98-99	0.07983602957124702
1213	100-101	0.12101446822477868
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	181.0
15	5492.0
16	5011.0
17	5491.0
18	4434.0
19	3282.0
20	4063.0
21	3086.0
22	3828.0
23	5650.0
24	11221.0
25	25711.0
26	57796.0
27	117224.0
28	205413.0
29	316087.0
30	436208.0
31	566195.0
32	705762.0
33	879377.0
34	1118232.0
35	1480835.0
36	2094545.0
37	2771339.0
38	1720881.0
39	192717.0
40	2172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.600220055586796	26.450265593123966	13.542569258846662	46.40694509244258
2	17.69097515719139	26.83325335828492	24.006916311252528	31.46885517327116
3	11.551951580085278	17.865809118169228	30.885866917762662	39.69637238398283
4	16.76856761363797	19.650761732546957	14.055027924801388	49.52564272901368
5	20.912518840655796	23.34514635312965	17.82551206737225	37.9168227388423
6	16.843609394181787	31.007645331714834	21.124084630888436	31.024660643214947
7	10.235428229456941	36.42883246961299	27.912344328573784	25.423394972356284
8	9.17556208099701	38.75358420393789	24.059970938451034	28.01088277661407
9	9.375876278161018	43.741783201414655	25.03060062697346	21.851739893450873
10-11	9.662580605114556	40.29949301736245	27.311723019478894	22.726203358044096
12-13	9.291641041430593	43.63485672572873	24.24813785576424	22.825364377076433
14-15	9.7416471632279	40.127637818513115	23.58768327662655	26.543031741632433
16-17	9.788243574219706	42.01356415505255	23.75899942549608	24.43919284523167
18-19	12.944188194372435	35.50188643432442	24.709453958560125	26.844471412743015
20-21	15.735348680410299	35.57565733053914	24.638288308713214	24.05070568033735
22-23	9.10991345156148	38.71723474225801	24.68523563669563	27.487616169484884
24-25	9.132201613306377	38.68426075823868	24.596426610323118	27.58711101813183
26-27	9.268976451233096	38.7184627193861	27.554572972180292	24.457987857200514
28-29	9.285128244711704	38.22779536605215	24.281957124939108	28.20511926429704
30-31	9.17829652903643	35.473913954591886	27.769046823783068	27.57874269258862
32-33	9.26826778760897	38.716116494156125	25.611192724405885	26.404422993829012
34-35	9.47120073955683	38.24691291788589	25.449311192295877	26.832575150261402
36-37	9.37281693040893	38.2364955407679	25.74635782569682	26.644329703126356
38-39	9.33598762319978	38.32836831716767	25.813990099571605	26.52165396006095
40-41	9.407653369761423	38.20226053710657	25.703236447707155	26.686849645424854
42-43	9.351921706261798	38.042778526496775	25.907601316786817	26.69769845045461
44-45	9.428922623520057	38.007537201711536	25.87493221868017	26.68860795608824
46-47	9.487615892785144	37.63979679639092	25.83598791172525	27.036599399098687
48-49	9.384279681540319	38.22755823782205	25.866091373150773	26.522070707486854
50-51	9.40618857261507	38.040524143234336	25.86316426726648	26.690123016884122
52-53	9.351515713212429	38.04056379049576	25.93131948128093	26.67660101501088
54-55	9.226947577231314	38.192513982816145	26.18430805891951	26.396230381033032
56-57	9.24344826587263	38.02060550128397	26.009195706738897	26.72675052610451
58-59	9.263176139346346	38.06472106419145	26.099704551901503	26.572398244560695
60-61	9.233956416907665	38.33926951511069	26.091070599991223	26.335703467990417
62-63	9.173930870938058	38.27044158180884	26.070385542764114	26.485242004488985
64-65	9.203137116665127	38.11783516319186	26.03459375920827	26.644433960934744
66-67	9.161335911930973	38.29954558978659	26.225990472262044	26.313128026020394
68-69	9.081629328417872	38.317243011681384	26.29170692804708	26.30942073185366
70-71	9.083341978623546	38.21979942442655	26.29284816178209	26.40401043516782
72-73	9.050637259090596	38.36506665494708	26.414857895473727	26.1694381904886
74-75	9.002853374193736	38.547011010050504	26.228261108488972	26.22187450726679
76-77	9.019924405171109	38.16165764097849	26.30790450624695	26.51051344760345
78-79	8.957935619311744	38.56469516594326	26.3512559232787	26.12611329146629
80-81	9.027966198757913	38.55054602460098	26.373909867463418	26.04757790917769
82-83	8.91688582532097	38.29731732974891	26.357749921110045	26.42804692382008
84-85	8.868947430093769	38.46204955147106	26.564945101194816	26.104057917240358
86-87	8.98991136465549	38.88627638157876	26.30332560984481	25.820486643920948
88-89	9.201888874801618	38.750558280362306	26.26371717555639	25.783835669279686
90-91	9.321769257601984	38.849560999810926	26.21990095106326	25.60876879152383
92-93	10.027710672194114	38.636832890990725	25.98764488631228	25.34781155050288
94-95	10.353840345228498	39.117011373317474	25.542651396949427	24.9864968845046
96-97	10.836416329848415	39.37405559781482	25.322533731218638	24.466994341118127
98-99	11.2530713478952	39.0989376193158	24.807925153006146	24.84006587978286
100-101	11.537588235429945	39.27471938997385	24.26010584787985	24.927586526716354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3915.0
1	3907.5
2	3722.5
3	3250.0
4	2965.0
5	2988.5
6	3063.0
7	3116.5
8	3113.5
9	3202.5
10	3508.0
11	3843.0
12	4075.0
13	5310.0
14	6925.0
15	8110.0
16	9723.5
17	12230.5
18	15768.5
19	20054.5
20	26030.0
21	35161.0
22	49648.0
23	73054.0
24	108448.5
25	159957.0
26	230190.5
27	317078.5
28	414342.5
29	511684.0
30	600950.0
31	673275.5
32	730067.5
33	768040.0
34	779118.0
35	771641.5
36	749310.5
37	714765.0
38	673894.5
39	626775.5
40	578318.5
41	532633.0
42	488385.0
43	443934.5
44	399528.5
45	358847.5
46	323464.0
47	291752.5
48	262282.0
49	243460.0
50	236243.5
51	219053.5
52	180136.0
53	141067.5
54	115844.0
55	98173.0
56	84781.0
57	74074.0
58	65458.0
59	58411.0
60	52591.5
61	47518.0
62	43327.0
63	39363.5
64	35661.5
65	32917.5
66	30339.0
67	28273.5
68	26629.0
69	24737.5
70	22644.0
71	20862.5
72	19409.5
73	18045.0
74	16908.0
75	15662.0
76	14560.5
77	13836.5
78	13188.0
79	12425.0
80	11771.0
81	11335.5
82	10940.0
83	10305.5
84	9752.0
85	9611.0
86	9457.0
87	9367.0
88	7924.5
89	6478.0
90	5944.5
91	5414.0
92	4952.0
93	4305.0
94	3987.5
95	3839.0
96	3759.0
97	3695.5
98	3669.0
99	3667.0
100	3667.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005878095211487038
3	0.002087887833014185
4	0.017201708362817397
5	3.6152551191226563E-4
6	5.5040256443562825E-5
7	0.0
8	7.868507792576693E-6
9	0.0
10-11	2.3616647957689357E-5
12-13	0.0
14-15	9.846399321433422E-5
16-17	1.5759237927379698E-4
18-19	2.049418333694412E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.3729222050506145E-5
28-29	1.584965273014627E-5
30-31	7.94224431701643E-6
32-33	3.9809204039248845E-6
34-35	1.5967606197954086E-4
36-37	0.0
38-39	0.0
40-41	3.422470021578472E-4
42-43	0.0025365056887602508
44-45	0.002014148726119825
46-47	2.7644018220660243E-4
48-49	0.005196494381259858
50-51	0.0020509489282195302
52-53	8.216315877907189E-6
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.724658229526435E-4
66-67	0.0
68-69	3.3983305106658515E-5
70-71	0.0
72-73	0.0
74-75	5.6250521128626036E-5
76-77	3.665063463191768E-4
78-79	0.0
80-81	0.0
82-83	3.4917831403906776E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	6.43931085207399E-5
94-95	4.623562701960109E-6
96-97	5.587314523856273E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	18375.0
5-9	20061.0
10-14	9810.0
15-19	11546.0
20-24	23656.0
25-29	60751.0
30-34	81676.0
35-39	89121.0
40-44	99454.0
45-49	102400.0
50-54	108782.0
55-59	117662.0
60-64	126142.0
65-69	150095.0
70-74	190953.0
75-79	230725.0
80-84	286139.0
85-89	87893.0
90-94	128777.0
95-99	297718.0
100-102	1.0500499E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.1528288140778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.35725257368462	64.77787395764591
2	7.845894741781476	11.635905793611897
3	2.0772680577201363	4.621059080552193
4	0.8127379840907636	2.410672824199243
5	0.4794163027179383	1.7775037513060685
6	0.3325679611249889	1.4796513054208906
7	0.23722462491117313	1.2313613901065426
8	0.1426196749429931	0.8460521873253751
9	0.11375899065189499	0.7591995863885019
>10	0.5704542594319101	7.158295388809593
>50	0.020651027304365896	1.0276533529561558
>100	0.009152646276620845	1.2224638217276074
>500	7.20075981388354E-4	0.3709496439284705
>1k	2.1599080070598017E-4	0.27340355303403374
>5k	4.380194221736732E-5	0.18340154008681384
>10k+	2.1286636856731846E-5	0.2245528229006647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	17465	0.13706386673923374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.334145854318336E-4	7.847916790107858E-6	7.847916790107858E-6
2	1.4126250222194143E-4	7.847916790107858E-6	7.847916790107858E-6
3	1.4126250222194143E-4	7.847916790107858E-6	7.847916790107858E-6
4	1.4126250222194143E-4	7.847916790107858E-6	7.847916790107858E-6
5	1.491104190120493E-4	7.847916790107858E-6	2.3543750370323573E-5
6	1.491104190120493E-4	7.847916790107858E-6	2.3543750370323573E-5
7	1.491104190120493E-4	7.847916790107858E-6	2.3543750370323573E-5
8	1.5695833580215717E-4	1.5695833580215716E-5	2.3543750370323573E-5
9	1.64806252592265E-4	1.5695833580215716E-5	3.139166716043143E-5
10-11	1.64806252592265E-4	1.5695833580215716E-5	3.139166716043143E-5
12-13	1.8442604456753468E-4	1.5695833580215716E-5	3.139166716043143E-5
14-15	2.040458365428043E-4	2.3543750370323573E-5	3.5315625555485365E-5
16-17	2.1189375333291216E-4	2.3543750370323573E-5	3.923958395053929E-5
18-19	2.1189375333291216E-4	2.3543750370323573E-5	3.923958395053929E-5
20-21	2.1974167012302003E-4	2.3543750370323573E-5	3.923958395053929E-5
22-23	2.4328542049334358E-4	2.3543750370323573E-5	4.708750074064715E-5
24-25	2.7467708765377505E-4	2.3543750370323573E-5	5.493541753075501E-5
26-27	3.0606875481420647E-4	2.3543750370323573E-5	5.493541753075501E-5
28-29	3.4530833876474576E-4	2.3543750370323573E-5	5.493541753075501E-5
30-31	3.7277604753012324E-4	2.3543750370323573E-5	5.493541753075501E-5
32-33	3.923958395053929E-4	2.3543750370323573E-5	5.8859375925808935E-5
34-35	4.002437562955007E-4	2.3543750370323573E-5	6.278333432086286E-5
36-37	4.237875066658243E-4	2.3543750370323573E-5	6.278333432086286E-5
38-39	4.5517917382625574E-4	3.139166716043143E-5	6.278333432086286E-5
40-41	4.8264688259163323E-4	3.139166716043143E-5	6.278333432086286E-5
42-43	5.258104249372265E-4	3.139166716043143E-5	7.063125111097072E-5
44-45	5.807458424679815E-4	3.139166716043143E-5	7.063125111097072E-5
46-47	6.278333432086286E-4	3.139166716043143E-5	7.063125111097072E-5
48-49	6.670729271591679E-4	3.923958395053929E-5	7.063125111097072E-5
50-51	7.259323030849769E-4	3.923958395053929E-5	7.063125111097072E-5
52-53	7.965635541959475E-4	4.708750074064715E-5	7.063125111097072E-5
54-55	8.750427220970262E-4	4.708750074064715E-5	7.063125111097072E-5
56-57	9.535218899981048E-4	4.708750074064715E-5	7.063125111097072E-5
58-59	0.0010280770995041294	4.708750074064715E-5	7.063125111097072E-5
60-61	0.0011301000177755315	4.708750074064715E-5	7.063125111097072E-5
62-63	0.0012831343951826349	4.708750074064715E-5	7.063125111097072E-5
64-65	0.0014518646061699538	5.493541753075501E-5	7.063125111097072E-5
66-67	0.0016205948171572728	5.493541753075501E-5	7.063125111097072E-5
68-69	0.0018403364872802926	5.493541753075501E-5	7.063125111097072E-5
70-71	0.002413234412958166	5.493541753075501E-5	7.847916790107858E-5
72-73	0.0037316844336962862	5.493541753075501E-5	7.847916790107858E-5
74-75	0.006074287595543482	7.063125111097072E-5	7.847916790107858E-5
76-77	0.009193834519611355	7.847916790107858E-5	7.847916790107858E-5
78-79	0.012301609568494068	7.847916790107858E-5	7.847916790107858E-5
80-81	0.016096077336511215	7.847916790107858E-5	7.847916790107858E-5
82-83	0.02066356490835399	7.847916790107858E-5	7.847916790107858E-5
84-85	0.025866733740195498	7.847916790107858E-5	7.847916790107858E-5
86-87	0.6545358800869706	7.847916790107858E-5	8.24031262961325E-5
88-89	1.5812296665380916	7.847916790107858E-5	8.632708469118644E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	126920	0.0	56.6818	5
TCATAAA	135245	0.0	54.42502	7
ATCATAA	138965	0.0	53.404728	6
TAAATCA	127185	0.0	52.75088	3
AAATCAT	135065	0.0	52.17672	4
CATAAAC	145040	0.0	50.73615	8
ATAAATC	139655	0.0	48.07076	2
AGAGCAC	13210	0.0	43.984783	94-95
AATAAAT	153670	0.0	43.70723	1
ATAAACA	181745	0.0	40.63338	9
GAGCACA	8315	0.0	34.709343	94-95
AACCGGG	955	0.0	33.20529	1
ACCGGGC	1000	0.0	32.61497	2
AGCCCGA	1015	0.0	32.122482	1
TACGGCG	1250	0.0	31.82191	6
AGCCGCC	1200	0.0	31.636614	1
AAACGGG	118250	0.0	31.548527	14-15
AGCCCCC	1240	0.0	31.336454	1
CACGGGC	1340	0.0	31.011187	3
CAAACGG	117455	0.0	30.740738	14-15
>>END_MODULE