##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 20150414_trimmed_2112_lane1_CAGATC_L001_R1_003.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6056165 Sequences flagged as poor quality 0 Sequence length 1-101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.525607542066638 30.0 25.0 31.0 16.0 34.0 2 29.5060843794851 31.0 28.0 33.0 25.0 34.0 3 26.102911330966112 30.0 22.0 31.0 10.0 34.0 4 30.509185734752236 35.0 28.0 35.0 19.0 37.0 5 32.45116284839553 35.0 32.0 37.0 25.0 37.0 6 34.10316221802616 35.0 33.0 37.0 30.0 37.0 7 34.818205828429726 35.0 35.0 37.0 32.0 37.0 8 34.996428913393736 35.0 35.0 37.0 32.0 37.0 9 36.77130612150214 39.0 35.0 39.0 32.0 39.0 10-11 36.967778909561325 39.0 37.0 39.0 33.0 39.0 12-13 36.9300028898748 39.0 37.0 39.0 33.0 39.0 14-15 38.06768107159949 40.0 37.0 41.0 33.0 41.0 16-17 38.010699338651776 40.0 37.0 41.0 33.0 41.0 18-19 38.137176727019614 40.0 37.0 41.0 33.0 41.0 20-21 38.07385733255034 40.0 37.0 41.0 33.0 41.0 22-23 38.18909854825502 40.0 37.0 41.0 33.0 41.0 24-25 38.12273276577956 40.0 37.0 41.0 33.0 41.0 26-27 37.9034962623714 40.0 37.0 41.0 33.0 41.0 28-29 37.854492893891106 40.0 37.0 41.0 32.5 41.0 30-31 37.90506586532348 40.0 37.0 41.0 33.0 41.0 32-33 37.779278192316106 40.0 37.0 41.0 33.0 41.0 34-35 37.745186308090425 40.0 37.0 41.0 32.0 41.0 36-37 37.745462996015064 40.0 37.0 41.0 32.0 41.0 38-39 37.78243634282984 40.0 37.0 41.0 33.0 41.0 40-41 37.79070106703928 40.0 37.0 41.0 33.0 41.0 42-43 37.66567857136555 40.0 37.0 41.0 32.0 41.0 44-45 37.569573572893226 40.0 36.5 41.0 32.0 41.0 46-47 37.56876684792839 40.0 36.5 41.0 32.0 41.0 48-49 37.51522943613514 39.5 36.0 41.0 32.0 41.0 50-51 37.419890776192005 39.0 36.0 41.0 31.5 41.0 52-53 37.260255863506686 39.0 36.0 40.5 31.5 41.0 54-55 37.13254298424154 39.0 35.0 40.0 31.0 41.0 56-57 36.921516406177005 39.0 35.0 40.0 31.0 41.0 58-59 36.68381560223209 38.0 35.0 40.0 31.0 41.0 60-61 36.52708615811724 38.0 35.0 40.0 31.0 41.0 62-63 36.15812936688599 37.0 34.5 40.0 30.5 41.0 64-65 36.10818635731219 37.0 34.0 39.5 31.0 41.0 66-67 35.849006611410914 36.5 34.0 39.0 31.0 41.0 68-69 35.60489431583419 36.0 34.0 39.0 31.0 40.5 70-71 35.3499538413741 35.5 34.0 38.5 31.0 40.0 72-73 35.14003138498655 35.0 34.0 37.0 31.0 39.5 74-75 34.8655237258767 35.0 34.0 37.0 31.0 39.0 76-77 32.806439147207286 33.5 31.0 35.5 27.5 37.0 78-79 34.21315586476547 35.0 33.5 36.0 30.5 37.5 80-81 34.244716058943396 35.0 34.0 36.0 31.0 37.0 82-83 34.102742466240244 35.0 34.0 35.5 31.0 37.0 84-85 33.9486305364468 35.0 34.0 35.0 31.0 36.5 86-87 33.81648924780751 35.0 33.0 35.0 31.0 36.0 88-89 33.729657579968716 35.0 33.0 35.0 31.0 36.0 90-91 33.66285525004386 35.0 33.0 35.0 31.0 36.0 92-93 33.61805813622996 35.0 33.0 35.0 31.0 36.0 94-95 33.59057777471935 35.0 33.0 35.0 31.0 35.5 96-97 33.557749368449635 35.0 33.0 35.0 30.5 35.0 98-99 33.58953383903898 35.0 33.0 35.0 31.0 35.0 100-101 32.9172311097768 34.5 32.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2302 1 -5.080869600270816 2302 2 -2.2570871118484135 2302 3 -3.951467892642871 2302 4 -3.6907703719519382 2302 5 -0.7644403233621553 2302 6 -0.6732457545696491 2302 7 -0.41252102030092885 2302 8 -0.13919441195798754 2302 9 -0.2595707532579965 2302 10-11 -0.30798922054120936 2302 12-13 -0.3249523019219538 2302 14-15 -0.590618158088013 2302 16-17 -0.27390811069864895 2302 18-19 -0.15791927587935817 2302 20-21 -0.15578023997822044 2302 22-23 -0.21847769628718794 2302 24-25 -0.2713878092490276 2302 26-27 -0.2928900108792618 2302 28-29 -0.3225524404973541 2302 30-31 -0.3543541609226182 2302 32-33 -0.5605272624504849 2302 34-35 -0.39769714252874167 2302 36-37 -0.352230086659695 2302 38-39 -0.35498685836449084 2302 40-41 -0.2705796003471548 2302 42-43 -0.20371227743665798 2302 44-45 -0.22762817653040202 2302 46-47 -0.14328856290111958 2302 48-49 -0.2157649392163421 2302 50-51 -0.13498198035820508 2302 52-53 -0.2193445026176022 2302 54-55 -0.23622816464616392 2302 56-57 -0.151600166621229 2302 58-59 -0.12042854722594143 2302 60-61 -0.10435181720168174 2302 62-63 -0.13684068776250058 2302 64-65 -0.1619365621572726 2302 66-67 -0.11457662450058592 2302 68-69 -0.06432220832950009 2302 70-71 -0.09622172994134104 2302 72-73 -0.1671592014911667 2302 74-75 -0.130597345327935 2302 76-77 0.07590643473181302 2302 78-79 -0.12080825566889786 2302 80-81 -0.11927411619829087 2302 82-83 -0.0017800079662322332 2302 84-85 -0.027183140712509157 2302 86-87 -0.04984448071473224 2302 88-89 -0.13960942072050386 2302 90-91 -0.09924692050390149 2302 92-93 -0.13050603143155115 2302 94-95 -0.11117681915332156 2302 96-97 -0.17419606992704217 2302 98-99 -0.1560710862239958 2302 100-101 -0.021687577587748308 2306 1 -3.1650882729204497 2306 2 -1.527908914880463 2306 3 -4.207315806143299 2306 4 0.3425893581876842 2306 5 0.2900552423182603 2306 6 0.017523429543572888 2306 7 -0.10879722422064475 2306 8 -0.09765364415061839 2306 9 -0.23132943984089138 2306 10-11 -0.34622202968357385 2306 12-13 -0.27509007120386997 2306 14-15 -0.3119997514893882 2306 16-17 -0.3396910648669689 2306 18-19 -0.3781116826473152 2306 20-21 -0.28627387067750476 2306 22-23 -0.4443932692791819 2306 24-25 -0.39610958388785633 2306 26-27 -0.3856958068924783 2306 28-29 -0.4681514822519617 2306 30-31 -0.4243008525430767 2306 32-33 -0.507389937367023 2306 34-35 -0.48280430138402153 2306 36-37 -0.5373442859646858 2306 38-39 -0.5024903198925088 2306 40-41 -0.4210630701393896 2306 42-43 -0.3142682126864642 2306 44-45 -0.3263304912106406 2306 46-47 -0.35080098129134996 2306 48-49 -0.31440170586367344 2306 50-51 -0.34164949726280724 2306 52-53 -0.30814872781727587 2306 54-55 -0.30299734687911695 2306 56-57 -0.1777975964163403 2306 58-59 -0.3078539508047413 2306 60-61 -0.33013264204515735 2306 62-63 -0.28155532582977116 2306 64-65 -0.19831098448748463 2306 66-67 -0.21005029659128382 2306 68-69 -0.1991822621429975 2306 70-71 -0.1563823487388234 2306 72-73 -0.15000797712716718 2306 74-75 -0.13086207185755683 2306 76-77 -0.14506910013892593 2306 78-79 -0.08172881602153126 2306 80-81 -0.08174680438582271 2306 82-83 -0.03608152118713548 2306 84-85 -0.050788763138641 2306 86-87 -0.059907209576437026 2306 88-89 -0.02751744974317205 2306 90-91 -0.01848035258731784 2306 92-93 -0.02156806265140432 2306 94-95 -8.320878998659964E-4 2306 96-97 -0.02851818495940961 2306 98-99 -0.026315477598728876 2306 100-101 0.09917843729222398 2303 1 -3.574338679123308 2303 2 -1.7405816312978395 2303 3 -0.8327429526590251 2303 4 0.23224211981797893 2303 5 -5.340602712603573 2303 6 -2.4676897452283697 2303 7 -0.9367810504333463 2303 8 -0.715286300960571 2303 9 -0.5744015232511472 2303 10-11 -0.42531557509152407 2303 12-13 -0.5218591765885634 2303 14-15 -0.6804887081091238 2303 16-17 -0.7038376383156475 2303 18-19 -0.6783208528448554 2303 20-21 -1.3633060377528778 2303 22-23 -0.670763135440211 2303 24-25 -0.76365072968661 2303 26-27 -0.7622050863669756 2303 28-29 -0.7611443149715669 2303 30-31 -0.8869907775271813 2303 32-33 -0.5047014124121816 2303 34-35 -0.5608509418851355 2303 36-37 -0.7027140261928579 2303 38-39 -0.6702749406112005 2303 40-41 -0.7585945690609037 2303 42-43 -0.7015487812249006 2303 44-45 -0.7241109112085695 2303 46-47 -0.5842802206186946 2303 48-49 -0.8140837148296569 2303 50-51 -0.6444900737523014 2303 52-53 -0.6061127393176307 2303 54-55 -0.6664622988175708 2303 56-57 -0.6746053098049174 2303 58-59 -0.624645208743928 2303 60-61 -0.4435981690489754 2303 62-63 -0.5797043404159439 2303 64-65 -0.8178105823015471 2303 66-67 -0.48491608972121725 2303 68-69 -0.40857755332888956 2303 70-71 -0.2694440874612454 2303 72-73 -0.3098309684410552 2303 74-75 -0.3287948581808777 2303 76-77 -0.0627372297399873 2303 78-79 -0.3017779292898908 2303 80-81 -0.2341062274815897 2303 82-83 -0.2816621650504416 2303 84-85 -0.31132838938788154 2303 86-87 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0.5960834115588085 2307 46-47 0.46026001871747013 2307 48-49 0.5971745307181493 2307 50-51 0.5356446940646151 2307 52-53 0.6093311367113117 2307 54-55 0.5413266806165069 2307 56-57 0.4947304536731707 2307 58-59 0.506029506277045 2307 60-61 0.4255110101543096 2307 62-63 0.5621967738813112 2307 64-65 0.4552178068732786 2307 66-67 0.38560640524998036 2307 68-69 0.3611643075704478 2307 70-71 0.2623707860450608 2307 72-73 0.23268704992118927 2307 74-75 0.2016317774830796 2307 76-77 -0.3679467980849651 2307 78-79 0.08995780095363415 2307 80-81 0.1518094159920338 2307 82-83 0.13936494346517492 2307 84-85 0.1737152643948363 2307 86-87 0.16816739048935858 2307 88-89 0.15416989284098293 2307 90-91 0.09898677970775793 2307 92-93 0.08873280608057854 2307 94-95 0.11265470391852972 2307 96-97 0.11519091015873073 2307 98-99 0.15907919226438594 2307 100-101 -0.15095915475099986 2304 1 2.8872138270014247 2304 2 1.7744177493269753 2304 3 4.267551272161828 2304 4 1.2996495357948454 2304 5 1.9640202213913511 2304 6 1.0990828424803922 2304 7 0.6269662435074466 2304 8 0.45777170952267454 2304 9 0.508660775172487 2304 10-11 0.4885687879236116 2304 12-13 0.4644822902915706 2304 14-15 0.5431658242867528 2304 16-17 0.5956756656072386 2304 18-19 0.5317416610368042 2304 20-21 0.7147203017920134 2304 22-23 0.6190233646018726 2304 24-25 0.5565480132078093 2304 26-27 0.49780911136698336 2304 28-29 0.5408895558914253 2304 30-31 0.5676199310416905 2304 32-33 0.6350694264530929 2304 34-35 0.6772083655980552 2304 36-37 0.7007229372324346 2304 38-39 0.6131842684795927 2304 40-41 0.6237774737337034 2304 42-43 0.4603675978607171 2304 44-45 0.5836742518567419 2304 46-47 0.48201542365647043 2304 48-49 0.47140599686026974 2304 50-51 0.46421969139095864 2304 52-53 0.47621492402168286 2304 54-55 0.46193813051934995 2304 56-57 0.46830449970730825 2304 58-59 0.38129325382485035 2304 60-61 0.3651335767258672 2304 62-63 0.4602356750137204 2304 64-65 0.4662701060166583 2304 66-67 0.3229216758240412 2304 68-69 0.28171422080428954 2304 70-71 0.24850579896848757 2304 72-73 0.25295225700894974 2304 74-75 0.19140530531213784 2304 76-77 -0.2542386628456441 2304 78-79 0.11282515102324453 2304 80-81 0.15462472461449295 2304 82-83 0.09637803963187963 2304 84-85 0.09891126297230102 2304 86-87 0.10034818455528693 2304 88-89 0.09987500040413977 2304 90-91 0.10255380409108739 2304 92-93 0.08313308344797576 2304 94-95 0.09806626076598945 2304 96-97 0.09300469551973833 2304 98-99 0.12719844368865552 2304 100-101 -0.17799676133126496 2301 1 2.61116968287838 2301 2 1.8022196928527627 2301 3 3.425537877190351 2301 4 -0.8808352810306666 2301 5 0.963250578304482 2301 6 0.624273005921502 2301 7 0.3883064008708601 2301 8 0.3082268162489754 2301 9 0.45501586367201696 2301 10-11 0.4408293576851676 2301 12-13 0.4412838754492654 2301 14-15 0.4825313765239585 2301 16-17 0.5788853236980458 2301 18-19 0.5052649623522996 2301 20-21 0.6458041347001711 2301 22-23 0.570937727493515 2301 24-25 0.5512468174711174 2301 26-27 0.4767530349169249 2301 28-29 0.5902328969156443 2301 30-31 0.536319985735723 2301 32-33 0.5435490520392321 2301 34-35 0.5645661842235228 2301 36-37 0.628100750480364 2301 38-39 0.6155410136060055 2301 40-41 0.5564604155004389 2301 42-43 0.5036366727297334 2301 44-45 0.5513783810342829 2301 46-47 0.39279803938377 2301 48-49 0.48560830621782713 2301 50-51 0.45972750567457865 2301 52-53 0.50515706454987 2301 54-55 0.4511862137163263 2301 56-57 0.42905200323105674 2301 58-59 0.4032287043525713 2301 60-61 0.3729142419771705 2301 62-63 0.4812061545768387 2301 64-65 0.33944778948390564 2301 66-67 0.2847865975210695 2301 68-69 0.2518544828340197 2301 70-71 0.2305064301063382 2301 72-73 0.18640325112007616 2301 74-75 0.12261147998903965 2301 76-77 -0.15174562111919698 2301 78-79 0.06502663382766372 2301 80-81 0.12946415954731094 2301 82-83 0.13761197173169393 2301 84-85 0.1275890093646339 2301 86-87 0.14146169778950934 2301 88-89 0.10874036883416238 2301 90-91 0.09633400817334348 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18-19 -0.4690694432550302 2215 20-21 -0.6426689824720313 2215 22-23 -0.5845998618468187 2215 24-25 -0.6298395583358243 2215 26-27 -0.5051219053104461 2215 28-29 -0.6091248409068655 2215 30-31 -0.5036273561802389 2215 32-33 -0.5135557595499805 2215 34-35 -0.6294720305435533 2215 36-37 -0.6251839871426554 2215 38-39 -0.5855146242307256 2215 40-41 -0.5295330889437366 2215 42-43 -0.4444768639144243 2215 44-45 -0.540623382449084 2215 46-47 -0.476948619625297 2215 48-49 -0.46684422559188476 2215 50-51 -0.4902558146992817 2215 52-53 -0.4789403243213215 2215 54-55 -0.31863900389534905 2215 56-57 -0.37185111780835456 2215 58-59 -0.44035271901241657 2215 60-61 -0.39229457753557284 2215 62-63 -0.4048144159206686 2215 64-65 -0.3287855754091211 2215 66-67 -0.3538221217960924 2215 68-69 -0.2716220124740687 2215 70-71 -0.253796919200191 2215 72-73 -0.2150008815640092 2215 74-75 -0.1969625462963549 2215 76-77 -0.09614224414865902 2215 78-79 -0.13933727747180313 2215 80-81 -0.1373043504378515 2215 82-83 -0.13450205190449793 2215 84-85 -0.1486292588686453 2215 86-87 -0.1443522787906346 2215 88-89 -0.20103681811542629 2215 90-91 -0.18986557425988337 2215 92-93 -0.12611751333903953 2215 94-95 -0.12592695646381458 2215 96-97 -0.10996753647192037 2215 98-99 -0.16314609746093822 2215 100-101 0.02607442016643091 2214 1 -3.3683703355686987 2214 2 -5.526358353704495 2214 3 0.022156806718029998 2214 4 2.575840740492449 2214 5 -4.039114698560926 2214 6 -1.785401837180281 2214 7 -1.2044280434729018 2214 8 -0.9337214758586896 2214 9 -1.2712313319273036 2214 10-11 -1.0551850375937093 2214 12-13 -0.9055307020522818 2214 14-15 -0.8429975197331459 2214 16-17 -1.0145957198587467 2214 18-19 -0.8529500990810774 2214 20-21 -1.3985881043145696 2214 22-23 -1.055247745929428 2214 24-25 -0.9616229538737784 2214 26-27 -0.9742299822856779 2214 28-29 -1.275134407068883 2214 30-31 -1.2628719127360597 2214 32-33 -1.0091223144118047 2214 34-35 -1.0533152389795504 2214 36-37 -1.3590143545179458 2214 38-39 -1.1365217670831882 2214 40-41 -1.1553897781466915 2214 42-43 -1.058701394024304 2214 44-45 -1.101852733455999 2214 46-47 -0.9900141817687782 2214 48-49 -1.0028638228817215 2214 50-51 -0.9672399979399842 2214 52-53 -1.150630370461748 2214 54-55 -0.9593908317425246 2214 56-57 -1.0034065041347873 2214 58-59 -0.9352703531161808 2214 60-61 -0.7553399252362425 2214 62-63 -1.1282162967203675 2214 64-65 -0.8142938188779354 2214 66-67 -0.7839888554421535 2214 68-69 -0.5388971263108857 2214 70-71 -0.32204841975764964 2214 72-73 -0.20362838048455245 2214 74-75 -0.168488749437671 2214 76-77 0.016491152022020117 2214 78-79 -0.21141495195804083 2214 80-81 -0.30956817219925625 2214 82-83 -0.25297838693672503 2214 84-85 -0.27322997626318823 2214 86-87 -0.26392898390083985 2214 88-89 -0.3222723468148345 2214 90-91 -0.342819832582407 2214 92-93 -0.26585779855974323 2214 94-95 -0.4160281352500661 2214 96-97 -0.26075652961898044 2214 98-99 -0.2581988294238329 2214 100-101 -0.1864745386657276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 258.0 15 4237.0 16 4055.0 17 3547.0 18 2419.0 19 1839.0 20 2068.0 21 1620.0 22 1950.0 23 3038.0 24 5785.0 25 12688.0 26 26911.0 27 51784.0 28 87555.0 29 131661.0 30 179922.0 31 233833.0 32 294509.0 33 371074.0 34 481435.0 35 658163.0 36 981327.0 37 1398281.0 38 982287.0 39 131859.0 40 2059.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.24987017361647 27.989742683695045 13.621161246432353 45.13922589625613 2 17.637548789311744 27.096342853255422 24.06672432713764 31.199384030295196 3 12.502615777577564 17.7563028471717 30.855671014046333 38.8854103612044 4 17.24878021381673 19.891910465587863 13.935372601624069 48.92393671897134 5 20.446840774394335 23.41903090881905 18.516313171610836 37.61781514517578 6 16.75539846724145 31.22946725501963 21.086270546812756 30.928863730926164 7 10.207258561948644 36.40329023898528 28.107483348029294 25.28196785103679 8 9.16411028970579 38.74888222355264 23.945115561270384 28.141891925471192 9 9.354130489534592 43.99380625405957 24.94477326051512 21.70728999589072 10-11 9.639334426158861 40.41885936197332 27.361240716093544 22.58056549577428 12-13 9.238603390237419 43.95284857835947 24.132159206761575 22.676388824641535 14-15 9.681909966539099 40.25382188185441 23.50307865177857 26.561189499827925 16-17 9.782987752880944 42.2423990550042 23.685740783138726 24.288872408976133 18-19 13.052811635432818 35.500853899146385 24.63064847792197 26.815685987498828 20-21 15.957398324553035 35.552148459015655 24.564143130485643 23.926310085945666 22-23 9.045147829322667 38.88127140858968 24.585740674908386 27.487840087179265 24-25 9.067692051639805 38.84772881845154 24.521449925566035 27.56312920434262 26-27 9.211190607179157 38.867988053832356 27.585966316815885 24.33485502217261 28-29 9.217905868518933 38.34565733235612 24.19573301291186 28.240703786213082 30-31 9.127500599935857 35.48833524925527 27.825906847660757 27.55825730314811 32-33 9.229061179786301 38.872545721605874 25.544603664531802 26.35378943407602 34-35 9.409953438822713 38.37091329471999 25.42289848789594 26.796234778561356 36-37 9.325181018611318 38.3698959879702 25.71371301132381 26.591209982094664 38-39 9.30491535267052 38.46495348839371 25.75561450337076 26.47451665556501 40-41 9.347410671761974 38.31539985956892 25.672857953720786 26.66433151494832 42-43 9.33074227961285 38.14894610048511 25.850562361882513 26.669749258019525 44-45 9.366893916893456 38.118270256704214 25.818625948263602 26.696209878138728 46-47 9.459001958361243 37.72960248456447 25.79114125658534 27.020254300488943 48-49 9.355557410030132 38.32403604035066 25.84048509166827 26.47992145795094 50-51 9.392774573014439 38.16753103638407 25.810378339327688 26.629316051273804 52-53 9.336381125073972 38.1676013857135 25.863153540895745 26.632863948316782 54-55 9.224241389230626 38.271538116600475 26.15823055684543 26.345989937323477 56-57 9.239469703499996 38.072169513582054 25.985746769587546 26.702614013330404 58-59 9.253487518739506 38.12328693539937 26.04391863374792 26.579306912113204 60-61 9.24101004728148 38.39316133401209 26.087926030612763 26.277902588093667 62-63 9.174247125657221 38.33075516903785 26.016913624585076 26.478084080719853 64-65 9.198345696796055 38.16707897811449 26.024040058236697 26.610535266852764 66-67 9.188058100240273 38.38096648193244 26.15377376972398 26.27720164810331 68-69 9.110280335221276 38.37786615010879 26.245843301041344 26.266010213628594 70-71 9.107245291861238 38.28739021935855 26.25034895571952 26.355015533060683 72-73 9.059327648008182 38.47180015067231 26.377040211650403 26.09183198966911 74-75 9.03075852082163 38.55721037050107 26.220063477410736 26.191967631266564 76-77 9.071353884844719 38.19153888913674 26.30822632481234 26.42888090120621 78-79 9.01392241868939 38.591571922387075 26.333790649790075 26.06071500913346 80-81 9.077516829829918 38.561414400682 26.35627811728295 26.004790652205134 82-83 8.94869301018754 38.31149139707763 26.32813395913647 26.41168163359836 84-85 8.948821208238508 38.49099109959956 26.52822544844522 26.031962243716716 86-87 9.03399102565552 38.87792856418754 26.297438267297284 25.790642142859653 88-89 9.29379500400205 38.755508414592114 26.19664704287518 25.754049538530655 90-91 9.39987095848343 38.85201586524649 26.20922439513342 25.538888781136666 92-93 10.135722566382329 38.609392281135534 25.938897859175125 25.315987293307014 94-95 10.48075203379986 39.09063729162179 25.48523479686836 24.943375877709993 96-97 11.011082752034639 39.39197348555934 25.24422481565122 24.352718946754802 98-99 11.42619683056531 39.08737584091403 24.740413706874314 24.74601362164634 100-101 11.696794625588703 39.41908569968419 24.133606963861986 24.750512710865117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1805.0 1 1801.0 2 1749.5 3 1613.5 4 1533.0 5 1572.5 6 1636.5 7 1668.0 8 1669.5 9 1716.0 10 1880.5 11 2050.5 12 2177.5 13 2907.5 14 3809.0 15 4372.0 16 5162.5 17 6441.0 18 8318.0 19 10603.5 20 13641.5 21 18237.5 22 25377.0 23 36780.0 24 54346.5 25 79554.5 26 113268.5 27 155155.0 28 201936.5 29 248498.5 30 290323.0 31 324239.5 32 350781.5 33 367954.5 34 372982.5 35 367772.5 36 355933.0 37 339442.0 38 319661.0 39 297145.5 40 273364.5 41 250933.0 42 229064.5 43 207452.0 44 186468.0 45 167475.5 46 150617.0 47 135416.5 48 121952.5 49 113548.0 50 110990.5 51 102801.5 52 83507.5 53 64878.0 54 53105.0 55 45055.0 56 39283.0 57 34553.0 58 30863.5 59 27961.5 60 25208.0 61 22846.0 62 21182.0 63 19341.5 64 17500.5 65 16253.0 66 15027.5 67 13995.0 68 13234.5 69 12424.0 70 11439.5 71 10529.5 72 9897.0 73 9361.5 74 8909.5 75 8297.0 76 7760.5 77 7471.5 78 7191.5 79 6786.5 80 6421.5 81 6229.0 82 6068.5 83 5786.5 84 5526.0 85 5466.5 86 5417.0 87 5394.5 88 4549.5 89 3702.5 90 3360.5 91 3020.5 92 2735.0 93 2324.0 94 2112.0 95 2007.5 96 1954.5 97 1923.0 98 1909.5 99 1903.0 100 1903.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.632662523670099E-4 3 0.009875804328938271 4 0.007829807084455702 5 2.977209461571669E-4 6 8.275154857110419E-5 7 2.1526429156190472E-4 8 0.0 9 0.0 10-11 8.28735165329765E-6 12-13 0.0 14-15 0.0 16-17 4.1476273290482815E-5 18-19 3.402142353933997E-4 20-21 4.980863108863819E-5 22-23 2.907074531992148E-4 24-25 1.9952103313321097E-4 26-27 5.8278828059390784E-5 28-29 2.0017961115610983E-4 30-31 2.6747329926893695E-4 32-33 5.110850150543861E-4 34-35 7.729330928951318E-4 36-37 0.0 38-39 8.449818303557019E-6 40-41 5.931078495451456E-5 42-43 8.498157939285081E-4 44-45 0.0010144489065988366 46-47 0.007404983417027033 48-49 0.003027485797403738 50-51 0.008861897480235602 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.360050531241637E-5 60-61 1.4877449200074563E-4 62-63 1.493482311415133E-4 64-65 7.938955431057053E-5 66-67 0.0 68-69 0.0 70-71 5.370267887758179E-5 72-73 0.0 74-75 0.0 76-77 0.00176987323602254 78-79 2.2057015178534995E-4 80-81 0.0 82-83 7.295869976372606E-4 84-85 0.0 86-87 0.0 88-89 1.0479051756322416E-4 90-91 1.5286991773209833E-4 92-93 1.7255641660142277E-4 94-95 0.0 96-97 1.9356993580156293E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 10235.0 5-9 12177.0 10-14 4830.0 15-19 5244.0 20-24 10952.0 25-29 27645.0 30-34 37988.0 35-39 41723.0 40-44 44687.0 45-49 44386.0 50-54 47314.0 55-59 50307.0 60-64 56614.0 65-69 68450.0 70-74 82776.0 75-79 103162.0 80-84 135030.0 85-89 35458.0 90-94 48726.0 95-99 104683.0 100-102 5083778.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.58214962585683 #Duplication Level Percentage of deduplicated Percentage of total 1 94.79091256870387 83.9678280033244 2 3.433278386505189 6.082543594812459 3 0.800928509307147 2.128439071531805 4 0.34097247115413304 1.2081629783229426 5 0.19416693428431253 0.8599862212583442 6 0.09855593357124436 0.5238177872474384 7 0.0825199655114158 0.5116857152436945 8 0.052868003458061814 0.37465291141938784 9 0.036052495015319555 0.28742467570492447 >10 0.15703798436498598 2.265676799005615 >50 0.007958907559703231 0.46803649938212183 >100 0.004279687426074945 0.720343834481978 >500 3.0214659653630206E-4 0.16564883223192461 >1k 1.2866834215066806E-4 0.22027408115898084 >5k 3.733819993623515E-5 0.215478994873975 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC 7836 0.12938881288736354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.604839861529533E-5 0.0 0.0 2 6.604839861529533E-5 0.0 0.0 3 6.604839861529533E-5 0.0 0.0 4 6.604839861529533E-5 0.0 0.0 5 9.907259792294299E-5 0.0 0.0 6 9.907259792294299E-5 0.0 0.0 7 9.907259792294299E-5 0.0 0.0 8 9.907259792294299E-5 0.0 0.0 9 9.907259792294299E-5 0.0 1.6512099653823832E-5 10-11 9.907259792294299E-5 0.0 2.476814948073575E-5 12-13 9.907259792294299E-5 8.256049826911916E-6 3.3024199307647664E-5 14-15 1.073286477498549E-4 1.6512099653823832E-5 3.3024199307647664E-5 16-17 1.4035284705750256E-4 1.6512099653823832E-5 3.3024199307647664E-5 18-19 1.4860889688441447E-4 1.6512099653823832E-5 3.3024199307647664E-5 20-21 1.6512099653823831E-4 3.3024199307647664E-5 3.3024199307647664E-5 22-23 1.8988914601897404E-4 3.3024199307647664E-5 3.3024199307647664E-5 24-25 2.724496442880932E-4 3.3024199307647664E-5 3.3024199307647664E-5 26-27 3.1372989342265276E-4 3.3024199307647664E-5 3.3024199307647664E-5 28-29 3.1372989342265276E-4 3.3024199307647664E-5 3.3024199307647664E-5 30-31 3.219859432495647E-4 3.3024199307647664E-5 3.3024199307647664E-5 32-33 3.3024199307647663E-4 3.3024199307647664E-5 3.3024199307647664E-5 34-35 3.3024199307647663E-4 3.3024199307647664E-5 3.3024199307647664E-5 36-37 3.4675409273030044E-4 3.3024199307647664E-5 3.3024199307647664E-5 38-39 3.6326619238412426E-4 3.3024199307647664E-5 3.3024199307647664E-5 40-41 3.8803434186486003E-4 3.3024199307647664E-5 3.3024199307647664E-5 42-43 4.210585411725077E-4 3.3024199307647664E-5 3.3024199307647664E-5 44-45 4.4582669065324344E-4 4.128024913455958E-5 4.953629896147149E-5 46-47 4.5408274048015535E-4 4.953629896147149E-5 4.953629896147149E-5 48-49 5.448992885761863E-4 4.953629896147149E-5 4.953629896147149E-5 50-51 6.439718864991294E-4 6.604839861529533E-5 4.953629896147149E-5 52-53 7.017642352875128E-4 6.604839861529533E-5 4.953629896147149E-5 54-55 7.678126339028082E-4 6.604839861529533E-5 4.953629896147149E-5 56-57 8.916533813064869E-4 6.604839861529533E-5 4.953629896147149E-5 58-59 9.989820290563418E-4 6.604839861529533E-5 4.953629896147149E-5 60-61 0.0011228227764600205 6.604839861529533E-5 6.604839861529533E-5 62-63 0.0013787603210942898 6.604839861529533E-5 6.604839861529533E-5 64-65 0.0015851615667670878 6.604839861529533E-5 6.604839861529533E-5 66-67 0.001733770463651502 6.604839861529533E-5 6.604839861529533E-5 68-69 0.0020392443072472432 6.604839861529533E-5 8.256049826911916E-5 70-71 0.002741008542534756 6.604839861529533E-5 8.256049826911916E-5 72-73 0.003929879717610072 6.604839861529533E-5 8.256049826911916E-5 74-75 0.006043428473299523 6.604839861529533E-5 8.256049826911916E-5 76-77 0.009048630610295458 6.604839861529533E-5 8.256049826911916E-5 78-79 0.012301514242098754 6.604839861529533E-5 8.256049826911916E-5 80-81 0.016272674208843388 6.604839861529533E-5 8.256049826911916E-5 82-83 0.020458491471087727 6.604839861529533E-5 8.256049826911916E-5 84-85 0.025709339161003705 6.604839861529533E-5 8.256049826911916E-5 86-87 0.6732643512850128 6.604839861529533E-5 8.256049826911916E-5 88-89 1.6420209819250302 6.604839861529533E-5 8.256049826911916E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCATA 63060 0.0 57.935234 5 TCATAAA 66990 0.0 55.557278 7 ATCATAA 68860 0.0 54.521835 6 TAAATCA 61600 0.0 53.44431 3 AAATCAT 66340 0.0 53.359985 4 CATAAAC 71375 0.0 52.027596 8 ATAAATC 67170 0.0 48.00137 2 CACGGGC 775 0.0 47.46971 3 CACACGG 785 0.0 44.548264 1 CCGGGCA 705 0.0 43.910103 3 AGAGCAC 6615 0.0 43.877514 94-95 AACCGGG 625 0.0 43.757816 1 ACCGGGC 670 0.0 43.510998 2 AATAAAT 74420 0.0 43.30965 1 ATAAACA 89335 0.0 41.675316 9 CCAACGG 830 0.0 36.204056 2 TACGGCG 770 0.0 34.38594 6 GAGCACA 4080 0.0 34.191658 94-95 ACACGGG 1055 0.0 34.009327 2 ATACGGC 785 0.0 33.724693 5 >>END_MODULE