##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 20150414_trimmed_2112_lane1_GCCAAT_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13494573 Sequences flagged as poor quality 0 Sequence length 1-101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.496639130411907 31.0 28.0 31.0 16.0 34.0 2 30.080352891805816 31.0 30.0 33.0 25.0 34.0 3 27.07698920775007 31.0 25.0 31.0 16.0 34.0 4 31.571436885733707 35.0 30.0 37.0 19.0 37.0 5 32.617266897917446 35.0 32.0 37.0 25.0 37.0 6 34.140220437917925 35.0 33.0 37.0 30.0 37.0 7 34.81292905425919 35.0 35.0 37.0 32.0 37.0 8 34.98704690660739 35.0 35.0 37.0 32.0 37.0 9 36.64265698610593 39.0 35.0 39.0 32.0 39.0 10-11 36.77140233492226 39.0 36.0 39.0 32.5 39.0 12-13 36.769125120026125 39.0 36.0 39.0 32.5 39.0 14-15 37.92535677720278 40.0 37.0 41.0 33.0 41.0 16-17 37.85751667695503 40.0 37.0 41.0 33.0 41.0 18-19 37.95253303116286 40.0 37.0 41.0 33.0 41.0 20-21 37.868973102771825 40.0 37.0 41.0 32.5 41.0 22-23 37.99428032866701 40.0 37.0 41.0 33.0 41.0 24-25 37.88244427068835 40.0 37.0 41.0 32.5 41.0 26-27 37.63229744990938 40.0 37.0 41.0 32.0 41.0 28-29 37.51931302070861 39.5 36.5 41.0 32.0 41.0 30-31 37.614924515525026 40.0 37.0 41.0 32.0 41.0 32-33 37.397861724474524 39.0 36.5 41.0 31.0 41.0 34-35 37.44494044366928 39.0 36.0 41.0 31.0 41.0 36-37 37.33396405841076 39.0 36.0 41.0 31.0 41.0 38-39 37.401260913991294 39.0 36.0 41.0 31.0 41.0 40-41 37.52332109389258 39.5 36.5 41.0 32.0 41.0 42-43 37.403657297364305 39.0 36.0 41.0 31.0 41.0 44-45 37.277412952673345 39.0 36.0 41.0 31.0 41.0 46-47 37.39943575880805 39.0 36.0 40.5 31.5 41.0 48-49 37.32022383946776 39.0 36.0 40.0 31.5 41.0 50-51 37.16141428678622 39.0 36.0 40.0 31.0 41.0 52-53 37.04817727855605 39.0 35.5 40.0 31.0 41.0 54-55 36.96400508715266 39.0 35.0 40.0 31.0 41.0 56-57 36.785794186196625 38.5 35.0 40.0 31.0 41.0 58-59 36.57224005619781 38.0 35.0 40.0 31.0 41.0 60-61 36.421683329125656 38.0 35.0 40.0 30.5 41.0 62-63 36.202082525052575 37.5 34.0 40.0 30.0 41.0 64-65 36.2217975184181 37.0 34.0 40.0 31.0 41.0 66-67 36.00045316814178 37.0 34.0 39.5 31.0 41.0 68-69 35.75803888191439 36.5 34.0 39.0 30.5 41.0 70-71 35.532007692831996 36.0 34.0 39.0 30.5 40.0 72-73 35.27494981122068 36.0 34.0 38.5 30.5 40.0 74-75 34.9536109165076 35.0 34.0 37.0 30.0 39.5 76-77 32.13344086632462 33.0 30.5 35.0 26.5 37.0 78-79 34.18084747060222 35.0 33.0 36.0 29.5 39.0 80-81 34.28532675897175 35.0 34.0 36.0 30.0 38.0 82-83 34.125997251319575 35.0 34.0 36.0 30.0 37.0 84-85 33.96386501216706 35.0 33.0 35.5 30.0 37.0 86-87 33.81882297286562 35.0 33.0 35.0 30.0 36.5 88-89 33.66113232025779 35.0 33.0 35.0 30.0 36.0 90-91 33.58415443781712 35.0 33.0 35.0 30.0 36.0 92-93 33.502457421978924 35.0 33.0 35.0 30.0 36.0 94-95 33.44245817560489 35.0 33.0 35.0 30.0 36.0 96-97 33.44322577043138 35.0 33.0 35.0 30.0 35.5 98-99 33.48494157093829 35.0 33.0 35.0 30.0 35.0 100-101 32.71966221064527 34.5 32.0 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.5961509212952656 1101 2 1.5197087192007892 1101 3 3.1360168425388544 1101 4 2.677507912055784 1101 5 2.9193005411894504 1101 6 1.4267689346174137 1101 7 0.6950328031595845 1101 8 0.5133936712391574 1101 9 0.5952505173261926 1101 10-11 0.5578190556460285 1101 12-13 0.5424814393922759 1101 14-15 0.4949206955904728 1101 16-17 0.491029791394908 1101 18-19 0.3520590792957847 1101 20-21 0.5724543759853233 1101 22-23 0.3777973731316635 1101 24-25 0.48110078424308256 1101 26-27 0.4896123498219396 1101 28-29 0.6714877147521889 1101 30-31 0.635145630488914 1101 32-33 0.6530105068246286 1101 34-35 0.5840680783604668 1101 36-37 0.6678562284457641 1101 38-39 0.623765330648574 1101 40-41 0.5661003978458794 1101 42-43 0.44325795465576334 1101 44-45 0.46897852675969887 1101 46-47 0.4082388797480334 1101 48-49 0.43656566690941645 1101 50-51 0.3264844912035514 1101 52-53 0.37350730956514155 1101 54-55 0.2645985487452336 1101 56-57 0.23330457874128285 1101 58-59 0.17626140569522875 1101 60-61 0.20863173492302423 1101 62-63 0.2948805070468694 1101 64-65 0.24858910643188636 1101 66-67 0.28931321558491874 1101 68-69 0.25000526483410823 1101 70-71 0.233669230699185 1101 72-73 0.16836652591053536 1101 74-75 0.12872025708425383 1101 76-77 -0.8511807736217278 1101 78-79 -0.06872354981774009 1101 80-81 0.08874979499472602 1101 82-83 0.08359752092220418 1101 84-85 0.0647631095732919 1101 86-87 0.07246570510063322 1101 88-89 0.04268557733253431 1101 90-91 0.03549128687355818 1101 92-93 0.039392555019666986 1101 94-95 0.05569175241109292 1101 96-97 0.06732816972378686 1101 98-99 0.054244622616757 1101 100-101 -0.3137843773859288 1102 1 2.743449329078164 1102 2 1.708904911141996 1102 3 3.915662105988993 1102 4 3.09931693049025 1102 5 3.1154995601620357 1102 6 1.6465847035481929 1102 7 0.799183527969106 1102 8 0.5546155164994815 1102 9 0.644571235774869 1102 10-11 0.6025813355002825 1102 12-13 0.5456586886607511 1102 14-15 0.44659305016313056 1102 16-17 0.5518954008514854 1102 18-19 0.48432091663975996 1102 20-21 0.7075855256715187 1102 22-23 0.5890436986439127 1102 24-25 0.6312142011375599 1102 26-27 0.6637696365599055 1102 28-29 0.7540895304826236 1102 30-31 0.6951097627590386 1102 32-33 0.7910965116189743 1102 34-35 0.8159861205096632 1102 36-37 1.0201780085852974 1102 38-39 0.883264972970494 1102 40-41 0.7529128000468361 1102 42-43 0.6158660265758726 1102 44-45 0.7465275523640216 1102 46-47 0.6179201444766917 1102 48-49 0.6371383295754356 1102 50-51 0.6335404604837436 1102 52-53 0.6265271588826096 1102 54-55 0.5557246280583215 1102 56-57 0.49264451026415657 1102 58-59 0.47677474647270657 1102 60-61 0.44258362910178306 1102 62-63 0.4885493014560609 1102 64-65 0.32807591163694383 1102 66-67 0.5019135624737814 1102 68-69 0.4777146827510279 1102 70-71 0.37233138326735826 1102 72-73 0.27227447251728876 1102 74-75 0.2358988430148159 1102 76-77 0.0555388582589913 1102 78-79 0.22930343857178315 1102 80-81 0.291477803612068 1102 82-83 0.2622290094554245 1102 84-85 0.29491294500358123 1102 86-87 0.3239860170368729 1102 88-89 0.20949668599541127 1102 90-91 0.21922677069523644 1102 92-93 0.1609648254648235 1102 94-95 0.14053805244938644 1102 96-97 0.19832754972199496 1102 98-99 0.20996475795602265 1102 100-101 -0.013803530256829788 1103 1 1.5122204201833966 1103 2 0.7870436364501003 1103 3 1.8988273290866715 1103 4 -0.6744731200135305 1103 5 0.7277501736626562 1103 6 0.3995806120979495 1103 7 0.2604074559615839 1103 8 0.22540236515160217 1103 9 0.30953039866986387 1103 10-11 0.3305653637971986 1103 12-13 0.2726478203336953 1103 14-15 0.3106187512440073 1103 16-17 0.28149349431740944 1103 18-19 0.30840404599113924 1103 20-21 0.4053639946361116 1103 22-23 0.396242452972821 1103 24-25 0.4102359735762917 1103 26-27 0.36339620255201055 1103 28-29 0.5077525685316573 1103 30-31 0.4577218103056211 1103 32-33 0.4344815998868512 1103 34-35 0.4439232952281458 1103 36-37 0.5293031027901662 1103 38-39 0.43645219335601837 1103 40-41 0.404058079993483 1103 42-43 0.28912819566970427 1103 44-45 0.2461160157134401 1103 46-47 0.2862007053174338 1103 48-49 0.2715076187776191 1103 50-51 0.1622450801156532 1103 52-53 0.26261477509505937 1103 54-55 0.23822174874930369 1103 56-57 0.21046405807798862 1103 58-59 0.1356360937562755 1103 60-61 0.09233472365984596 1103 62-63 0.24030203885671853 1103 64-65 0.17867244064564147 1103 66-67 0.10995987168249144 1103 68-69 0.1797988917074278 1103 70-71 0.014019905688790857 1103 72-73 -0.06223443799141393 1103 74-75 -0.06043216291676856 1103 76-77 -0.5754073968018112 1103 78-79 -0.167389370881736 1103 80-81 -0.07922237581632174 1103 82-83 -0.09392632109676669 1103 84-85 -0.1298244923094174 1103 86-87 -0.03562268492481735 1103 88-89 -0.11711233890896722 1103 90-91 -0.009337439624133026 1103 92-93 -0.07228983222612584 1103 94-95 -0.15700867157965348 1103 96-97 -0.0648094211133099 1103 98-99 -0.06007935385517982 1103 100-101 -0.28952047298806605 1216 1 0.981927713284243 1216 2 0.33658613537993887 1216 3 1.0850707077726724 1216 4 -0.3978771693920926 1216 5 1.171710574239718 1216 6 0.5199934856154158 1216 7 0.2540864224114401 1216 8 0.2059565343486085 1216 9 0.1949702382338998 1216 10-11 0.2341475060451117 1216 12-13 0.2391536203432878 1216 14-15 0.20723413104670385 1216 16-17 0.18151443938322842 1216 18-19 0.1865633953482444 1216 20-21 0.275238417306376 1216 22-23 0.1554699835581559 1216 24-25 0.22098749224777237 1216 26-27 0.18718475491672137 1216 28-29 0.28188359359975124 1216 30-31 0.27611138295601734 1216 32-33 0.2903197859078759 1216 34-35 0.23792302891342842 1216 36-37 0.39637786702898126 1216 38-39 0.36127113966190905 1216 40-41 0.19455230615701424 1216 42-43 0.13857144813229638 1216 44-45 0.13593015035195322 1216 46-47 0.1669606234577401 1216 48-49 0.2893791519132378 1216 50-51 0.19699792395041982 1216 52-53 0.2166253968908336 1216 54-55 0.15620057241223861 1216 56-57 0.10116509373491311 1216 58-59 0.14691994728246982 1216 60-61 0.08467474410789322 1216 62-63 0.16235251263928774 1216 64-65 0.08649986798735654 1216 66-67 0.06851462979425094 1216 68-69 0.01164995763444665 1216 70-71 0.010243829278998362 1216 72-73 -0.06388823167399238 1216 74-75 -0.13251570665553203 1216 76-77 -0.5787270700394629 1216 78-79 -0.14787959074322998 1216 80-81 0.030393940904900774 1216 82-83 -0.004657267351795724 1216 84-85 0.019465932393089247 1216 86-87 -0.0029037358656438528 1216 88-89 -0.07562857572978743 1216 90-91 -0.05838056623561272 1216 92-93 -0.039796727891221906 1216 94-95 -0.10674064550946127 1216 96-97 -0.051665988435551924 1216 98-99 -0.03479604947811765 1216 100-101 -0.2178262942099778 1113 1 2.467516940317889 1113 2 1.3257970520864326 1113 3 2.5620785113496645 1113 4 3.375022992779737 1113 5 2.8928774360734053 1113 6 1.2233931228814043 1113 7 0.5542222914068518 1113 8 0.3398951623721871 1113 9 0.23485052143188767 1113 10-11 0.20305286887036544 1113 12-13 0.14220089402132174 1113 14-15 0.17186581944048385 1113 16-17 0.12410973506545275 1113 18-19 0.15440350167638428 1113 20-21 0.2515699508502891 1113 22-23 0.12155865657128828 1113 24-25 0.08009239426640136 1113 26-27 0.1400572268857161 1113 28-29 0.1658363784703596 1113 30-31 0.2545399213024453 1113 32-33 0.38962577650308106 1113 34-35 0.23061158578147456 1113 36-37 0.12724409099937617 1113 38-39 0.10251837210716275 1113 40-41 0.15323788985686093 1113 42-43 0.018351615664670362 1113 44-45 0.19101667711057502 1113 46-47 0.13904788674733481 1113 48-49 0.07632589065988782 1113 50-51 -0.024828434141639377 1113 52-53 0.10552015334815223 1113 54-55 -0.039180086171249684 1113 56-57 0.05800354867908908 1113 58-59 -0.019431706311230812 1113 60-61 -0.1321725988432405 1113 62-63 0.017783803749338745 1113 64-65 -0.14387662546414504 1113 66-67 0.014132838443835283 1113 68-69 -0.057600522433517654 1113 70-71 -0.1005487328425616 1113 72-73 -0.093599905759568 1113 74-75 -0.08373172359078751 1113 76-77 -0.6017248595523412 1113 78-79 -0.13815720766992712 1113 80-81 -0.022224027473569663 1113 82-83 0.03785884856461763 1113 84-85 0.05024983003990968 1113 86-87 0.01960036690597633 1113 88-89 0.018046439812934523 1113 90-91 -0.03275658150426608 1113 92-93 0.06065025916226574 1113 94-95 -0.018671743852898715 1113 96-97 -0.08497013234721607 1113 98-99 -0.07146672868135795 1113 100-101 -0.17688796726076106 1112 1 2.161893957210495 1112 2 1.1510928002352152 1112 3 1.8136125115830986 1112 4 3.1072070244851524 1112 5 2.3777358215947118 1112 6 1.007531821574652 1112 7 0.4598885475020751 1112 8 0.295923818242386 1112 9 0.1695302089237316 1112 10-11 0.1414820173874105 1112 12-13 0.08330329958730687 1112 14-15 2.6614615078557335E-4 1112 16-17 -0.01989869600034666 1112 18-19 0.061496499072134725 1112 20-21 0.19288981634573332 1112 22-23 0.07058601686479449 1112 24-25 0.08469792852718427 1112 26-27 0.0911412641116982 1112 28-29 -0.006747121435715542 1112 30-31 0.05795685280363472 1112 32-33 0.17270256245269877 1112 34-35 0.04830129320677656 1112 36-37 0.17273464225433344 1112 38-39 0.10394926667791538 1112 40-41 0.11543175559861396 1112 42-43 0.008823534012876166 1112 44-45 0.05721276686338683 1112 46-47 -0.04532779460976144 1112 48-49 -0.027736311322627216 1112 50-51 -0.09982118180110433 1112 52-53 -0.10524692741453379 1112 54-55 -0.04694546153540813 1112 56-57 -0.12346758908185507 1112 58-59 -0.15191790875654476 1112 60-61 -0.1561472025814723 1112 62-63 -0.14472194448894982 1112 64-65 -0.059700701927845046 1112 66-67 -0.08030570595747832 1112 68-69 -0.04951175085815862 1112 70-71 -0.04050792486142285 1112 72-73 -0.14989588283528832 1112 74-75 -0.1162811488969453 1112 76-77 -0.6643772880634984 1112 78-79 -0.15051887877898906 1112 80-81 -0.028063674934635685 1112 82-83 -0.057514033142545884 1112 84-85 -0.022821873234398993 1112 86-87 -0.08626756003798164 1112 88-89 -0.0989641607057763 1112 90-91 -0.017482262675443394 1112 92-93 -0.005410071612210743 1112 94-95 -0.02495721573062326 1112 96-97 -0.04529138675201949 1112 98-99 -0.03258989084370256 1112 100-101 -0.11624254178335747 1115 1 1.4671159168354357 1115 2 0.8147460828683855 1115 3 0.1956662015723012 1115 4 0.11709233994623602 1115 5 1.6745836237143408 1115 6 0.7375411182348373 1115 7 0.28138137693957077 1115 8 0.12600594672196763 1115 9 0.08928043847832612 1115 10-11 0.1207144666110267 1115 12-13 -0.04060194036525644 1115 14-15 -0.08173900891694785 1115 16-17 -0.09455559219215814 1115 18-19 0.02356915216517308 1115 20-21 0.055429413360506885 1115 22-23 -0.09027982545005386 1115 24-25 -0.03181629897143523 1115 26-27 -0.09849155531195919 1115 28-29 -0.06048547553604777 1115 30-31 0.08572682518657615 1115 32-33 0.10170613773019 1115 34-35 -0.07047036478671487 1115 36-37 0.16169954290288047 1115 38-39 0.14072737455251882 1115 40-41 0.024753924899620472 1115 42-43 -0.06788385523289264 1115 44-45 -0.04162072691903518 1115 46-47 -0.0010630302874190534 1115 48-49 -0.020201360116892886 1115 50-51 -0.11881412259232604 1115 52-53 -0.011440788386266831 1115 54-55 -0.10211283062123044 1115 56-57 -0.15592475988198373 1115 58-59 -0.07650819394527275 1115 60-61 -0.06031315840824192 1115 62-63 0.037399648611120995 1115 64-65 -0.13574966183284687 1115 66-67 -0.03272860533937916 1115 68-69 -0.049353017829815826 1115 70-71 -0.10198821315817241 1115 72-73 -0.20518985470680917 1115 74-75 -0.21608340206697108 1115 76-77 -0.4032161857229255 1115 78-79 -0.17102646956377754 1115 80-81 -0.11574486226557212 1115 82-83 -0.11061224640315714 1115 84-85 -0.049605833847969905 1115 86-87 -0.09335221418254491 1115 88-89 -0.05244818873122625 1115 90-91 -0.04187769332268232 1115 92-93 -0.06403931198214963 1115 94-95 -0.03987303132777953 1115 96-97 -0.03421669093464885 1115 98-99 -0.013918746672196392 1115 100-101 -0.006599473055601379 1114 1 1.9726542074942763 1114 2 1.0002231350230666 1114 3 3.477651170446382 1114 4 0.49005947806460526 1114 5 1.1394984352189255 1114 6 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92-93 0.050380734423697504 1212 94-95 0.06483533567131161 1212 96-97 0.003837655165746412 1212 98-99 0.08198156952847313 1212 100-101 0.3897984826056913 1213 1 -2.85295118750901 1213 2 -1.6617399496675809 1213 3 -2.314759382928706 1213 4 0.0041249074893841 1213 5 -4.553428807867817 1213 6 -2.0698379254526564 1213 7 -0.9281877189523371 1213 8 -0.5457378975201408 1213 9 -0.49749724304147236 1213 10-11 -0.30966321109933403 1213 12-13 -0.5650214091337915 1213 14-15 -0.4622332821991506 1213 16-17 -0.5924698403191471 1213 18-19 -0.39889146833600364 1213 20-21 -0.6730105257637504 1213 22-23 -0.7185520961315746 1213 24-25 -0.7726899470442135 1213 26-27 -0.9359204028613846 1213 28-29 -0.7990060559832628 1213 30-31 -0.598936103850896 1213 32-33 -0.5105702698410965 1213 34-35 -0.7989781899814687 1213 36-37 -0.639867036628516 1213 38-39 -0.546284467065874 1213 40-41 -0.6639159672272044 1213 42-43 -0.5133823854901323 1213 44-45 -0.6025440656557564 1213 46-47 -0.7324670115731422 1213 48-49 -0.6719580257853295 1213 50-51 -0.5666177842779803 1213 52-53 -0.4493096876098548 1213 54-55 -0.4266311076516729 1213 56-57 -0.36360688659200946 1213 58-59 -0.38890743577897524 1213 60-61 -0.20898466463911092 1213 62-63 -0.36512867108871205 1213 64-65 -0.7061662387178487 1213 66-67 -0.6509583481099739 1213 68-69 -0.5168563809836542 1213 70-71 -0.26181059721373146 1213 72-73 -0.07110176360126985 1213 74-75 -0.08207017066946776 1213 76-77 0.6311127322604904 1213 78-79 0.016358612744419077 1213 80-81 -0.09764864309372001 1213 82-83 -0.1224009754758626 1213 84-85 -0.12121194872484153 1213 86-87 -0.0917638617571157 1213 88-89 -0.12413892282270922 1213 90-91 -0.14579006605549694 1213 92-93 -0.057369663555491 1213 94-95 0.004727368534908294 1213 96-97 -0.017200595853118728 1213 98-99 -0.053041336115597915 1213 100-101 0.1711286988959344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 247.0 15 8138.0 16 7950.0 17 7919.0 18 6008.0 19 4520.0 20 5512.0 21 4130.0 22 5107.0 23 7352.0 24 13441.0 25 28939.0 26 61801.0 27 119744.0 28 206076.0 29 312958.0 30 433631.0 31 563811.0 32 711419.0 33 892930.0 34 1144679.0 35 1521657.0 36 2119820.0 37 2846850.0 38 2141809.0 39 315233.0 40 2891.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.522841589726477 30.990354418772643 14.37440073131621 42.112403260184664 2 14.429389197572585 31.44339912920369 25.021550548634874 29.10566112458885 3 10.79889208075219 17.17056179487128 35.31859868034896 36.711947444027565 4 14.409579895240752 26.234443310771933 14.759946493293866 44.59603030069345 5 16.304474757205945 29.496202755111835 18.62544181914381 35.57388066853841 6 13.539974195890908 35.29788637025591 21.416905427843613 29.745234006009568 7 9.414405572815934 33.764877058235996 33.24923342788782 23.571483941060254 8 8.876122268396882 36.19654389699306 23.54137358298431 31.38596025162575 9 9.140873721043151 46.96982576194151 24.4821238494213 19.40717666759404 10-11 9.26290168743136 41.135440514502704 29.561272161319675 20.040385636746265 12-13 8.441686779891066 47.60337180363441 23.745505917510915 20.209435498963614 14-15 9.002216177500246 41.89334746271795 23.121928768190305 25.982507591591506 16-17 9.00245559187471 46.53525126767999 23.510001543238317 20.952291597206983 18-19 14.726620308216317 34.27780304910373 24.584061350510833 26.41151529216912 20-21 19.75626880043884 34.55468486812489 24.5116954008425 21.177350930593775 22-23 7.761032535100037 40.98873244289609 24.35834424882045 26.891890773183423 24-25 7.727309853779392 40.74866164033947 23.962476355098488 27.56155215078265 26-27 7.7796313912654345 40.70658266749422 29.582233834681652 21.931552106558694 28-29 7.898120344099036 40.29429964708752 23.596593015910525 28.210986992902914 30-31 7.741250727826037 35.08792814912641 29.78374806500203 27.387073058045523 32-33 7.825198039456955 41.19103121843141 25.98144263964422 25.002328102467413 34-35 7.874866694021952 40.93129360646628 25.721982757398447 25.471856942113323 36-37 7.950718397296313 40.19198966064194 26.325776350000684 25.531515592061066 38-39 7.842514396043654 40.527419641770365 26.3382609529025 25.291805009283486 40-41 7.845239843000816 40.72197173380852 25.961585639068733 25.47120278412192 42-43 7.808600180108282 40.44890677413651 26.22911451741344 25.513378528341768 44-45 7.886961835468111 40.26305111626566 26.34824049629767 25.50174655196856 46-47 7.866678105312744 39.9584451577997 26.321084544118396 25.853792192769166 48-49 7.877506619242823 40.239628046701775 26.36553330614729 25.517332027908108 50-51 7.850158462234452 40.04948330082313 26.252767937667414 25.847590299275 52-53 7.856203168012213 40.47530278629145 26.322537069665685 25.345956976030653 54-55 7.802055059380314 40.18613792365576 26.420854708009873 25.59095230895406 56-57 7.788436943658186 39.84408638773107 26.500312223761473 25.867164444849273 58-59 7.839915689848412 40.11257851568075 26.59820722709558 25.449298567375262 60-61 7.787784766716163 40.28915534930672 26.479390630673677 25.44366925330344 62-63 7.776694532602868 40.27947725512687 26.44565685850032 25.498171353769944 64-65 7.787814855101171 40.15891833914238 26.43415014968864 25.61911665606781 66-67 7.787103389974639 40.05364733659142 26.746594768724396 25.412654504709547 68-69 7.813720118242983 40.128019402690796 26.71649374202295 25.34176673704327 70-71 7.798312791681313 39.98091290286797 26.792997920780394 25.42777638467032 72-73 7.7810557186415625 39.965088916004554 26.85222785757705 25.40162750777683 74-75 7.764935202629411 40.17188634157879 26.80012722957598 25.263051226215815 76-77 7.791865490040191 39.88989121461779 26.877436164673373 25.44080713066864 78-79 7.80391317830353 40.08043569714204 26.85642003171955 25.259231092834877 80-81 7.819434670307918 40.19601551726557 26.929855925929953 25.05469388649656 82-83 7.777886230986727 40.184290043844996 26.65572394216198 25.3820997830063 84-85 7.762295159948277 40.08322878798709 26.96070618855409 25.193769863510546 86-87 7.836649588404282 40.53093981505023 26.867336566718485 24.765074029827 88-89 7.958963918916403 40.337653143222525 26.82618943025733 24.877193507603746 90-91 8.069973991236767 40.1780961537668 26.744090726379977 25.00783912861646 92-93 8.450980731292288 40.17896352250571 26.654363084641687 24.71569266156032 94-95 8.600449430334114 40.67361257982718 26.33189251482794 24.394045475010774 96-97 8.868609175269016 40.55143846519381 26.31624968127783 24.26370267825934 98-99 9.111738396444235 40.5828028989765 25.999880567817215 24.305578136762055 100-101 9.237059269411152 40.97559587781366 25.560396822529448 24.226948030245747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8003.0 1 7914.5 2 7276.5 3 6175.5 4 5657.0 5 5773.5 6 6000.0 7 6184.5 8 6378.0 9 7005.0 10 8334.5 11 10348.5 12 13088.5 13 18447.0 14 25935.5 15 34122.5 16 44663.0 17 58336.0 18 76258.5 19 99460.5 20 129844.0 21 169650.0 22 220175.0 23 283651.5 24 358130.0 25 438927.0 26 521656.0 27 602025.0 28 672883.0 29 727486.0 30 763311.5 31 776708.0 32 774864.5 33 757465.5 34 714890.0 35 661263.5 36 608652.0 37 559633.0 38 511673.5 39 461424.5 40 412706.0 41 369140.5 42 335942.5 43 308498.0 44 281663.5 45 258754.5 46 240083.0 47 222986.5 48 206569.5 49 192121.5 50 181680.0 51 169837.5 52 153884.5 53 139290.5 54 126987.0 55 116021.0 56 106195.0 57 96838.0 58 88423.0 59 80668.5 60 73563.0 61 67008.0 62 60764.5 63 54434.0 64 48563.5 65 44108.5 66 40007.0 67 36506.5 68 33580.0 69 30846.5 70 28025.0 71 25512.5 72 23545.5 73 21803.5 74 20311.0 75 18559.5 76 17134.0 77 16334.5 78 15651.5 79 14676.0 80 13806.0 81 13283.5 82 12801.5 83 12052.5 84 11365.5 85 11172.5 86 11014.5 87 10942.0 88 9217.5 89 7480.0 90 6833.0 91 6204.5 92 5622.5 93 4812.5 94 4453.5 95 4296.5 96 4203.0 97 4137.0 98 4113.5 99 4107.0 100 4107.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0052243270455407925 3 0.0022754386630678665 4 0.013204387534020203 5 4.7512198200104304E-4 6 4.4569228379634444E-5 7 0.0 8 1.9331310633864012E-4 9 7.436821966349273E-6 10-11 1.4878016076068322E-5 12-13 3.720651823969563E-6 14-15 6.699443819617921E-5 16-17 8.935509412833157E-5 18-19 2.7934327959290074E-4 20-21 7.453097100961367E-6 22-23 1.8645137648288043E-5 24-25 0.0 26-27 2.6180387658236132E-5 28-29 4.498181385265937E-5 30-31 3.757733509505901E-6 32-33 4.8992824360197056E-5 34-35 1.0964282978616094E-4 36-37 3.41428139278098E-5 38-39 0.0 40-41 2.17640304112997E-4 42-43 0.0017393690358367105 44-45 0.0026913966545401304 46-47 8.524907484502317E-4 48-49 0.004257313251406113 50-51 0.0015536604609454232 52-53 1.16935613380303E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.572506651550348E-5 66-67 4.2818442759665445E-4 68-69 1.6075667524008807E-5 70-71 0.0 72-73 0.0 74-75 4.0778693621926864E-5 76-77 3.0366368180313855E-4 78-79 0.0 80-81 0.0 82-83 2.6741815625543714E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.9894150790879653E-5 94-95 0.0 96-97 3.02259710870451E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 24348.0 5-9 26500.0 10-14 10883.0 15-19 13656.0 20-24 29844.0 25-29 74863.0 30-34 100861.0 35-39 107862.0 40-44 112472.0 45-49 108439.0 50-54 114381.0 55-59 118192.0 60-64 119327.0 65-69 133118.0 70-74 157717.0 75-79 177742.0 80-84 210257.0 85-89 90138.0 90-94 126631.0 95-99 280112.0 100-102 1.135723E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.84558341414868 #Duplication Level Percentage of deduplicated Percentage of total 1 90.64102645694825 73.27926665169268 2 6.4214307182592485 10.382886255424093 3 1.2293495968255352 2.9816245612592653 4 0.5994871892671787 1.9386356626245291 5 0.332122976760423 1.3425337910720072 6 0.15637006369434 0.7585097416725904 7 0.12088419500824843 0.6841067289694094 8 0.09426290033597823 0.6096591337577528 9 0.08381429470187997 0.609841399825699 >10 0.29611052210836564 3.9335413701508157 >50 0.014938193409745221 0.7851889092251598 >100 0.008824835167783866 1.3397087589396766 >500 9.379008470628543E-4 0.518263960356161 >1k 4.0263821175382745E-4 0.5996720550963451 >5k 2.8322328331926345E-5 0.1498579961727478 >10k+ 9.196125875559777E-6 0.0867030237610781 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1115579574099899E-4 0.0 0.0 2 1.1115579574099899E-4 0.0 7.4103863827332664E-6 3 1.1115579574099899E-4 0.0 7.4103863827332664E-6 4 1.1856618212373226E-4 0.0 7.4103863827332664E-6 5 1.2597656850646554E-4 0.0 7.4103863827332664E-6 6 1.2597656850646554E-4 0.0 7.4103863827332664E-6 7 1.3338695488919879E-4 0.0 7.4103863827332664E-6 8 1.3338695488919879E-4 0.0 7.4103863827332664E-6 9 1.3338695488919879E-4 0.0 1.4820772765466533E-5 10-11 1.4079734127193206E-4 0.0 1.4820772765466533E-5 12-13 1.445025344632987E-4 0.0 1.4820772765466533E-5 14-15 1.5932330722876522E-4 0.0 1.4820772765466533E-5 16-17 1.7414407999423177E-4 0.0 1.4820772765466533E-5 18-19 1.7784927318559838E-4 0.0 2.2231159148199798E-5 20-21 2.111960119078981E-4 0.0 2.2231159148199798E-5 22-23 2.2231159148199798E-4 0.0 2.2231159148199798E-5 24-25 2.482479438215644E-4 0.0 2.593635233956643E-5 26-27 2.815946825438641E-4 0.0 2.9641545530933066E-5 28-29 3.1864661445753044E-4 0.0 2.9641545530933066E-5 30-31 3.6310893275393004E-4 0.0 2.9641545530933066E-5 32-33 4.0386605785896297E-4 0.0 2.9641545530933066E-5 34-35 4.0757125105032963E-4 0.0 2.9641545530933066E-5 36-37 4.2609721700716285E-4 0.0 2.9641545530933066E-5 38-39 4.6314914892082917E-4 7.4103863827332664E-6 2.9641545530933066E-5 40-41 4.964958876431289E-4 7.4103863827332664E-6 2.9641545530933066E-5 42-43 5.298426263654285E-4 7.4103863827332664E-6 3.3346738722299696E-5 44-45 5.743049446618281E-4 1.4820772765466533E-5 4.0757125105032965E-5 46-47 6.150620697668611E-4 1.4820772765466533E-5 4.4462318296399595E-5 48-49 6.632295812546273E-4 1.8525965956833167E-5 5.187270467913286E-5 50-51 7.151022859337602E-4 2.2231159148199798E-5 5.187270467913286E-5 52-53 7.632697974215264E-4 2.2231159148199798E-5 5.187270467913286E-5 54-55 8.299632748661258E-4 2.2231159148199798E-5 5.5577897870499494E-5 56-57 8.633100135884255E-4 2.9641545530933066E-5 5.928309106186613E-5 58-59 9.151827182675583E-4 2.9641545530933066E-5 5.928309106186613E-5 60-61 0.0010115177412430908 2.9641545530933066E-5 5.928309106186613E-5 62-63 0.0011449046961322896 2.9641545530933066E-5 5.928309106186613E-5 64-65 0.0013264591625092546 2.9641545530933066E-5 6.298828425323276E-5 66-67 0.00146355131058982 3.3346738722299696E-5 6.669347744459939E-5 68-69 0.001667336936114985 3.7051931913666334E-5 6.669347744459939E-5 70-71 0.002074908187165315 3.7051931913666334E-5 6.669347744459939E-5 72-73 0.0028307675982041074 3.7051931913666334E-5 7.410386382733267E-5 74-75 0.004183163113052928 3.7051931913666334E-5 7.78090570186993E-5 76-77 0.005680061162365049 3.7051931913666334E-5 8.151425021006593E-5 78-79 0.007677160292511663 3.7051931913666334E-5 8.151425021006593E-5 80-81 0.01004848393498631 4.0757125105032965E-5 8.151425021006593E-5 82-83 0.012671760714473885 4.4462318296399595E-5 8.521944340143255E-5 84-85 0.01583599569990099 4.4462318296399595E-5 9.633502297553247E-5 86-87 0.38629232655231105 4.4462318296399595E-5 9.633502297553247E-5 88-89 0.9308890322057616 4.4462318296399595E-5 1.000402161668991E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCATA 218340 0.0 63.2607 5 ATAAATC 206150 0.0 62.22726 2 TAAATCA 206820 0.0 61.894836 3 TCATAAA 232885 0.0 61.487553 7 ATCATAA 235115 0.0 61.35157 6 AAATCAT 225275 0.0 60.51418 4 CATAAAC 236300 0.0 60.443184 8 AATAAAT 249695 0.0 51.22219 1 ATAAACA 288605 0.0 49.593784 9 CACACGG 1650 0.0 40.692978 1 ACACGGG 1890 0.0 36.01361 2 AGAGCAC 10100 0.0 35.67113 94-95 AACCGGG 1230 0.0 35.300327 1 AAACGGG 213380 0.0 34.247677 14-15 ACAAACG 219240 0.0 33.80481 12-13 ACGGGCA 218655 0.0 33.241413 16-17 CAAACGG 212920 0.0 33.23413 14-15 CCAACGG 1665 0.0 33.08078 2 AGAAATC 5205 0.0 32.606438 2 GGGCAAC 221275 0.0 32.454685 18-19 >>END_MODULE