##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_GCCAAT_L001_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7316692
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.863950949418125	30.0	25.0	31.0	16.0	34.0
2	29.753012396464353	31.0	30.0	33.0	25.0	34.0
3	26.53701993719297	30.0	23.0	31.0	16.0	34.0
4	30.448956846336863	35.0	28.0	35.0	19.0	37.0
5	32.48749031083246	35.0	32.0	37.0	22.0	37.0
6	34.16581820882381	35.0	33.0	37.0	30.0	37.0
7	34.895729742772765	35.0	35.0	37.0	32.0	37.0
8	35.08664015412643	36.0	35.0	37.0	32.0	37.0
9	36.874866281253006	39.0	37.0	39.0	33.0	39.0
10-11	37.07705292486253	39.0	37.0	39.0	33.0	39.0
12-13	37.057953228704655	39.0	37.0	39.0	33.0	39.0
14-15	38.254808997852976	40.0	37.5	41.0	33.0	41.0
16-17	38.20111807220805	40.0	37.0	41.0	33.0	41.0
18-19	38.283077518480106	40.0	38.0	41.0	33.5	41.0
20-21	38.20810764644126	40.0	37.5	41.0	33.0	41.0
22-23	38.29058550459355	40.0	38.0	41.0	34.0	41.0
24-25	38.18924367866819	40.0	38.0	41.0	33.0	41.0
26-27	38.0046700516733	40.0	37.0	41.0	33.0	41.0
28-29	37.92300133900466	40.0	37.0	41.0	33.0	41.0
30-31	38.02655167397076	40.0	37.0	41.0	33.0	41.0
32-33	37.916971745460366	40.0	37.0	41.0	33.0	41.0
34-35	37.878458737870204	40.0	37.0	41.0	33.0	41.0
36-37	37.84302102909625	40.0	37.0	41.0	33.0	41.0
38-39	37.88979853375136	40.0	37.0	41.0	33.0	41.0
40-41	37.94223801145779	40.0	37.0	41.0	33.0	41.0
42-43	37.83337964274586	40.0	37.0	41.0	33.0	41.0
44-45	37.74620087529446	40.0	37.0	41.0	32.5	41.0
46-47	37.76849404689118	40.0	37.0	41.0	33.0	41.0
48-49	37.71654241514398	40.0	37.0	41.0	32.5	41.0
50-51	37.65565650289706	40.0	36.5	41.0	32.0	41.0
52-53	37.50110772473582	39.0	36.0	41.0	32.0	41.0
54-55	37.403424331943505	39.0	36.0	41.0	32.0	41.0
56-57	37.23430857462321	39.0	35.5	40.5	31.5	41.0
58-59	37.0208523217719	39.0	35.0	40.0	31.0	41.0
60-61	36.89212367004919	38.5	35.0	40.0	31.0	41.0
62-63	36.58496450940811	38.0	35.0	40.0	31.0	41.0
64-65	36.58722596346343	37.5	35.0	40.0	31.0	41.0
66-67	36.34400325890963	37.0	35.0	40.0	31.0	41.0
68-69	36.095252313210054	37.0	34.5	39.0	31.0	41.0
70-71	35.84158341492779	36.0	34.0	39.0	31.0	40.5
72-73	35.59759382232546	36.0	34.0	39.0	31.0	40.0
74-75	35.29182509323647	35.5	34.0	37.5	31.0	40.0
76-77	33.094361388100026	33.5	31.5	35.5	27.5	38.0
78-79	34.54798231114172	35.0	34.0	36.0	30.5	39.0
80-81	34.55801149339108	35.0	34.0	36.0	31.0	38.5
82-83	34.372308857158885	35.0	34.0	36.0	31.0	37.0
84-85	34.17685924410999	35.0	34.0	36.0	31.0	37.0
86-87	34.023578530666974	35.0	34.0	35.0	31.0	36.5
88-89	33.881344174697446	35.0	34.0	35.0	31.0	36.0
90-91	33.80329446459693	35.0	34.0	35.0	31.0	36.0
92-93	33.74337161920773	35.0	33.5	35.0	31.0	36.0
94-95	33.69568700643447	35.0	33.0	35.0	31.0	36.0
96-97	33.66231868040865	35.0	33.0	35.0	31.0	36.0
98-99	33.6958767908427	35.0	33.0	35.0	31.0	35.0
100-101	33.048319847585006	34.5	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	-3.978375074088195
2306	2	-2.3207657270671085
2306	3	-2.9020525104010986
2306	4	-0.012696305651978435
2306	5	-5.271226420705748
2306	6	-2.405764236173269
2306	7	-1.1570577811403098
2306	8	-0.7165483558561974
2306	9	-0.9588438873107847
2306	10-11	-0.7960348370606312
2306	12-13	-0.7963929139839223
2306	14-15	-0.8092459086210155
2306	16-17	-0.9967539552818394
2306	18-19	-0.8404163171807824
2306	20-21	-1.130676044198239
2306	22-23	-1.1240771290769942
2306	24-25	-3.607384161286646
2306	26-27	-3.0035773460923707
2306	28-29	-1.4437209986237036
2306	30-31	-1.1369781528707037
2306	32-33	-1.2447967031117173
2306	34-35	-1.1137202250520843
2306	36-37	-1.2252014038656895
2306	38-39	-1.0831643252045424
2306	40-41	-1.2051418828104303
2306	42-43	-0.8706096210348235
2306	44-45	-0.8961215418038293
2306	46-47	-0.831389798004146
2306	48-49	-1.0139516995057747
2306	50-51	-1.0704664261697303
2306	52-53	-0.8665267928178366
2306	54-55	-0.7538779729790548
2306	56-57	-0.7950447931391622
2306	58-59	-0.8383211272820361
2306	60-61	-0.7916198412008058
2306	62-63	-0.806982025648459
2306	64-65	-0.8767220677186458
2306	66-67	-0.7380786415491372
2306	68-69	-0.8385425623029903
2306	70-71	-0.4917710044788848
2306	72-73	-0.4827650216296604
2306	74-75	-0.408048731093146
2306	76-77	-0.3864867920631596
2306	78-79	-0.3921784058030511
2306	80-81	-0.34393864187585166
2306	82-83	-0.36326538158287747
2306	84-85	-0.47176674620254033
2306	86-87	-0.4081697889677187
2306	88-89	-0.3093210202639227
2306	90-91	-0.36242409859244873
2306	92-93	-0.36296289540712223
2306	94-95	-0.42874431676186475
2306	96-97	-0.38618992587356615
2306	98-99	-0.3179876646228763
2306	100-101	-0.11151370934652505
2201	1	3.6701193681566586
2201	2	2.2578018875304338
2201	3	4.525672335951079
2201	4	4.209090207297031
2201	5	3.1722776947150777
2201	6	1.6342966291493966
2201	7	0.8667352393468377
2201	8	0.5747969065988059
2201	9	0.6838136075251882
2201	10-11	0.5397029943448359
2201	12-13	0.5292054027221198
2201	14-15	0.5393931503518345
2201	16-17	0.5747305818823918
2201	18-19	0.5073004338497711
2201	20-21	0.7551045678583463
2201	22-23	0.7119634201172644
2201	24-25	0.7547114092140248
2201	26-27	0.6945676229786031
2201	28-29	0.7616672268899762
2201	30-31	0.6774394045566439
2201	32-33	0.7199361280672605
2201	34-35	0.6952874416172747
2201	36-37	-0.03176570050395355
2201	38-39	0.18052610686140014
2201	40-41	0.5276779137897165
2201	42-43	0.5335153230698566
2201	44-45	0.6420112390220538
2201	46-47	0.6300175232822625
2201	48-49	0.5718741625663455
2201	50-51	0.5532727952341219
2201	52-53	0.5847228392890074
2201	54-55	0.5447925461298126
2201	56-57	0.5475511092797163
2201	58-59	0.5768969704951843
2201	60-61	0.4659837447499129
2201	62-63	0.5677462748783384
2201	64-65	0.4881689118161532
2201	66-67	0.4209000172044455
2201	68-69	0.37034500379099455
2201	70-71	0.3402811716741425
2201	72-73	0.27005165577122625
2201	74-75	0.2040661509762458
2201	76-77	-0.48460954569413417
2201	78-79	0.09879126170318386
2201	80-81	0.1772216100196431
2201	82-83	0.21220558374399445
2201	84-85	0.2090631583266287
2201	86-87	0.21176764876874898
2201	88-89	0.16812967862092165
2201	90-91	0.18326425225527032
2201	92-93	0.2059534291043903
2201	94-95	0.18828813130847522
2201	96-97	0.18794809327810924
2201	98-99	0.21276867367041774
2201	100-101	-0.15042369434736003
2307	1	3.227853321487377
2307	2	1.9843719110446365
2307	3	4.6238643300130065
2307	4	3.3183851763715495
2307	5	2.7930810670966366
2307	6	1.5019773848744293
2307	7	0.7300901156242787
2307	8	0.4622016043472428
2307	9	0.6052599849352589
2307	10-11	0.4629456484931964
2307	12-13	0.4339719420204773
2307	14-15	0.4259710271473338
2307	16-17	0.5534961417558932
2307	18-19	0.47439543492708225
2307	20-21	0.7073986044144931
2307	22-23	0.5950658838567122
2307	24-25	0.6561971962710231
2307	26-27	0.6345344383658045
2307	28-29	0.7331142115644056
2307	30-31	0.6514958558697401
2307	32-33	0.6982324607632648
2307	34-35	0.7194414636924904
2307	36-37	0.5934554861682599
2307	38-39	0.5870598310483572
2307	40-41	0.6478785777021514
2307	42-43	0.604436439740347
2307	44-45	0.6708672460609577
2307	46-47	0.5633863133081363
2307	48-49	0.5804522175108247
2307	50-51	0.6512056668302506
2307	52-53	0.7134880843354949
2307	54-55	0.6087700820995963
2307	56-57	0.5997820552891184
2307	58-59	0.5663107703467674
2307	60-61	0.5387141204766124
2307	62-63	0.6292899554280993
2307	64-65	0.5429433610556416
2307	66-67	0.48912764474660264
2307	68-69	0.4200707471166609
2307	70-71	0.33194126953199543
2307	72-73	0.2663441057882707
2307	74-75	0.26624521815995195
2307	76-77	-0.08414145166096176
2307	78-79	0.17574440452720808
2307	80-81	0.24068551499760815
2307	82-83	0.2373899179217247
2307	84-85	0.22689079289968817
2307	86-87	0.24705596533870988
2307	88-89	0.19959777235348497
2307	90-91	0.22544352225032327
2307	92-93	0.18274004778435682
2307	94-95	0.12495731743606342
2307	96-97	0.19213027497050916
2307	98-99	0.2334987859684503
2307	100-101	7.567911030292862E-4
2202	1	2.608930168986568
2202	2	1.6077015233523788
2202	3	3.3725098776644877
2202	4	1.2184936404101627
2202	5	1.4544653784768542
2202	6	0.767902638492231
2202	7	0.4246215060859839
2202	8	0.3036957553008861
2202	9	0.46159878225140005
2202	10-11	0.38621549937239763
2202	12-13	0.4334555047724251
2202	14-15	0.4292434883140359
2202	16-17	0.5058632099346312
2202	18-19	0.47813669940658343
2202	20-21	0.64053802982761
2202	22-23	0.5539152774895584
2202	24-25	0.5987957952817453
2202	26-27	0.575259975912779
2202	28-29	0.6132473634066713
2202	30-31	0.5442477867497075
2202	32-33	0.5505349736655347
2202	34-35	0.6308015217819403
2202	36-37	0.6266744976912904
2202	38-39	0.5696741940736771
2202	40-41	0.5825791170757952
2202	42-43	0.48603077795365124
2202	44-45	0.5006454827734359
2202	46-47	0.4657271070680764
2202	48-49	0.5219257975115141
2202	50-51	0.4805168172585823
2202	52-53	0.4810131104798714
2202	54-55	0.4640019669174933
2202	56-57	0.46802963569901834
2202	58-59	0.4713605148730764
2202	60-61	0.4176144095562577
2202	62-63	0.4831658186547898
2202	64-65	0.41964028100360196
2202	66-67	0.4168879085728605
2202	68-69	0.2969686296512819
2202	70-71	0.267239112924905
2202	72-73	0.20408006500864673
2202	74-75	0.12075474433871136
2202	76-77	-0.2590598455977897
2202	78-79	0.11080108718933701
2202	80-81	0.17375897639178817
2202	82-83	0.1735561774573
2202	84-85	0.171719531883177
2202	86-87	0.17470615051514216
2202	88-89	0.09735459049851869
2202	90-91	0.09344430158225236
2202	92-93	0.14687954473945553
2202	94-95	0.1278380739507483
2202	96-97	0.14200839986499147
2202	98-99	0.17696738346712237
2202	100-101	-0.07932199993094713
2304	1	2.5035754809053827
2304	2	1.378486262679953
2304	3	3.0009569983680926
2304	4	0.9298027027140812
2304	5	1.6002784359483613
2304	6	0.8664352107900157
2304	7	0.4772923835767813
2304	8	0.347531403990331
2304	9	0.4653065550777882
2304	10-11	0.3753411786367735
2304	12-13	0.3983391572467596
2304	14-15	0.4253357821507322
2304	16-17	0.4805406050759302
2304	18-19	0.47688498761753806
2304	20-21	0.6040832613519598
2304	22-23	0.5309665851396588
2304	24-25	0.5848125633915231
2304	26-27	0.5432415509630744
2304	28-29	0.5931781785666459
2304	30-31	0.547186215481311
2304	32-33	0.4937915303682274
2304	34-35	0.5642392045112956
2304	36-37	0.6830321496128349
2304	38-39	0.5877301117294209
2304	40-41	0.5374289085326396
2304	42-43	0.503991723270083
2304	44-45	0.4708467648581234
2304	46-47	0.4264880086913223
2304	48-49	0.41885972965431506
2304	50-51	0.424529344661309
2304	52-53	0.5027441509933794
2304	54-55	0.43702747197318104
2304	56-57	0.4124412486079265
2304	58-59	0.44932129366785745
2304	60-61	0.39254192735712934
2304	62-63	0.4514882187089668
2304	64-65	0.45304355683302333
2304	66-67	0.36266580893901335
2304	68-69	0.2777709217181368
2304	70-71	0.23450269113964595
2304	72-73	0.17283643520590886
2304	74-75	0.14660639253278163
2304	76-77	-0.08236871107161647
2304	78-79	0.1106400293139842
2304	80-81	0.12644816417552818
2304	82-83	0.12292101702331593
2304	84-85	0.11239591737755461
2304	86-87	0.12528225594528664
2304	88-89	0.09837501759815837
2304	90-91	0.13796311440238895
2304	92-93	0.1163100434642601
2304	94-95	0.08646315002842897
2304	96-97	0.11863057901105378
2304	98-99	0.14749576557615285
2304	100-101	-0.013328828979496166
2203	1	0.529610197549335
2203	2	0.39972126296169463
2203	3	-0.7028262775641423
2203	4	-2.113542249377822
2203	5	-0.1655373348771505
2203	6	-0.012017614553855083
2203	7	0.07549319050925618
2203	8	0.12894876970202063
2203	9	0.23361448378921068
2203	10-11	0.2785769248417225
2203	12-13	0.3067060212639916
2203	14-15	0.3375284798218985
2203	16-17	0.38546790042169476
2203	18-19	0.33571895561230747
2203	20-21	0.4034597540775948
2203	22-23	0.39946343614590774
2203	24-25	0.5092413759890206
2203	26-27	0.48288527840171014
2203	28-29	0.4097683877828757
2203	30-31	0.4120676512750805
2203	32-33	0.42558795430311136
2203	34-35	0.402353951275245
2203	36-37	0.4480578330084768
2203	38-39	0.4276689349115159
2203	40-41	0.4216043075559739
2203	42-43	0.3736209524791505
2203	44-45	0.3126933565350143
2203	46-47	0.3048119575045689
2203	48-49	0.2895481182076054
2203	50-51	0.35386082468811253
2203	52-53	0.34852018137904395
2203	54-55	0.31288727087185464
2203	56-57	0.3236786874169866
2203	58-59	0.3332003188896806
2203	60-61	0.2814969781738341
2203	62-63	0.3011407863960187
2203	64-65	0.32580603493162386
2203	66-67	0.24752350612568108
2203	68-69	0.22036032828184915
2203	70-71	0.21661392596541162
2203	72-73	0.17850248735248186
2203	74-75	0.09037689159537621
2203	76-77	-0.12354535673964762
2203	78-79	0.07065660517707784
2203	80-81	0.06663812414694803
2203	82-83	0.07522458271677124
2203	84-85	0.0915365937635002
2203	86-87	0.13473177926322677
2203	88-89	0.07604648638984202
2203	90-91	0.03885445930042408
2203	92-93	0.017285844968476738
2203	94-95	0.02481613458230214
2203	96-97	-0.009608346978851046
2203	98-99	0.06698031474662969
2203	100-101	-0.0816771957427136
2305	1	2.0473503636872756
2305	2	1.3583259001507173
2305	3	2.9397551664331125
2305	4	1.819042766007371
2305	5	1.0294400235072771
2305	6	0.5977737028912458
2305	7	0.3692225887151679
2305	8	0.24999408440655913
2305	9	0.09501287192084362
2305	10-11	0.16985450769441712
2305	12-13	0.23746621757841524
2305	14-15	0.2568722013404994
2305	16-17	0.20607636975144317
2305	18-19	0.2666995172269182
2305	20-21	0.3982930621338312
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2214	68-69	-0.3138294118909428
2214	70-71	-0.3271759188506209
2214	72-73	-0.2985983100852607
2214	74-75	-0.2963183951366659
2214	76-77	0.14283418899422173
2214	78-79	-0.19770709938515552
2214	80-81	-0.24004567900745144
2214	82-83	-0.26878648474529854
2214	84-85	-0.2903496036819817
2214	86-87	-0.2568156663583423
2214	88-89	-0.21162439285799195
2214	90-91	-0.18685985967846364
2214	92-93	-0.16636526455616973
2214	94-95	-0.128761473251906
2214	96-97	-0.11600445693276384
2214	98-99	-0.1278023382321294
2214	100-101	-0.011110680903186676
2213	1	-3.0956804726014013
2213	2	-1.4686119635782369
2213	3	-2.9842109104966
2213	4	-1.098593994335559
2213	5	0.41235262251691296
2213	6	-0.10776304536744874
2213	7	-0.2022003048841725
2213	8	-0.4173526657406228
2213	9	-0.512738412938873
2213	10-11	-0.41801191970306206
2213	12-13	-0.6522490457605699
2213	14-15	-0.7516199775198089
2213	16-17	-0.5994918492618595
2213	18-19	-0.6382765723961867
2213	20-21	-0.8314568329635748
2213	22-23	-0.6908157335621112
2213	24-25	-0.6446442096169136
2213	26-27	-0.5247229848576325
2213	28-29	-0.75380447249821
2213	30-31	-0.6462922445815522
2213	32-33	-0.6500900514728656
2213	34-35	-0.7704732899145199
2213	36-37	-0.7464123059988523
2213	38-39	-0.7174198631470006
2213	40-41	-0.696819916608554
2213	42-43	-0.6134263788032186
2213	44-45	-0.6135418767756917
2213	46-47	-0.5885874602071652
2213	48-49	-0.6655585761881895
2213	50-51	-0.621498961866294
2213	52-53	-0.6262583036162255
2213	54-55	-0.4872798460057197
2213	56-57	-0.5349036761632036
2213	58-59	-0.5749498273485898
2213	60-61	-0.5316951145083806
2213	62-63	-0.5696282399944792
2213	64-65	-0.467602906215852
2213	66-67	-0.4708503159171755
2213	68-69	-0.36524673113874684
2213	70-71	-0.3459941657782508
2213	72-73	-0.2875841701857169
2213	74-75	-0.2530869348331777
2213	76-77	-0.046270503151127684
2213	78-79	-0.2195805765895429
2213	80-81	-0.23080701016112926
2213	82-83	-0.19684281820974547
2213	84-85	-0.1743016988593027
2213	86-87	-0.199242575199726
2213	88-89	-0.21975931543421723
2213	90-91	-0.21218848975496485
2213	92-93	-0.17192194629311786
2213	94-95	-0.15738344247601077
2213	96-97	-0.17552447063297194
2213	98-99	-0.18509938899740064
2213	100-101	0.036308975553907885
2212	1	-3.9508634724989165
2212	2	-5.722115562340839
2212	3	-0.5184697013796367
2212	4	2.378255076655684
2212	5	-4.23880287499756
2212	6	-1.9247990567586015
2212	7	-1.2513760089948107
2212	8	-0.9790565364561488
2212	9	-1.3822388014392644
2212	10-11	-1.1717541761031498
2212	12-13	-1.0334410558982228
2212	14-15	-0.9935836286109847
2212	16-17	-1.1849971163698854
2212	18-19	-1.0118864708934865
2212	20-21	-1.6011591579532336
2212	22-23	-1.2704446717075584
2212	24-25	-1.054412168182182
2212	26-27	-1.113727287525819
2212	28-29	-1.4757800923735118
2212	30-31	-1.4198836901617824
2212	32-33	-1.1856261872762701
2212	34-35	-1.2988179217007385
2212	36-37	-1.4801040133121504
2212	38-39	-1.2430963330637539
2212	40-41	-1.3900544263582617
2212	42-43	-1.252404947512126
2212	44-45	-1.3427032637665022
2212	46-47	-1.2747370773439357
2212	48-49	-1.235342260659067
2212	50-51	-1.1899223118901574
2212	52-53	-1.3613911612029597
2212	54-55	-1.1947393277343679
2212	56-57	-1.1803113480914433
2212	58-59	-1.1008096512205867
2212	60-61	-0.927181700984363
2212	62-63	-1.3197130658134455
2212	64-65	-1.1125721359671488
2212	66-67	-1.025063334378899
2212	68-69	-0.7543600668520298
2212	70-71	-0.44589997257174474
2212	72-73	-0.32030163851172944
2212	74-75	-0.2616855842280046
2212	76-77	0.026750464270314467
2212	78-79	-0.2689373594910478
2212	80-81	-0.3713053092754848
2212	82-83	-0.3284009000273045
2212	84-85	-0.3214908925977653
2212	86-87	-0.38472221027551967
2212	88-89	-0.41874435653353714
2212	90-91	-0.4349790042148953
2212	92-93	-0.39271195530238145
2212	94-95	-0.4753544143110915
2212	96-97	-0.34036964627262023
2212	98-99	-0.3532822870900958
2212	100-101	-0.22894542654064054
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	221.0
15	5562.0
16	5980.0
17	5180.0
18	3425.0
19	2680.0
20	2997.0
21	2229.0
22	2714.0
23	3837.0
24	6669.0
25	13972.0
26	28791.0
27	54508.0
28	92989.0
29	141355.0
30	196303.0
31	258498.0
32	330781.0
33	423273.0
34	554954.0
35	764823.0
36	1125400.0
37	1636059.0
38	1405207.0
39	245185.0
40	3099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.223447426787953	31.69912031284083	14.352086981384485	41.72534527898673
2	14.394386278736535	31.465204539130237	25.003102515306196	29.137306666827033
3	11.247019141340447	17.331614211438048	34.96280787965198	36.458558767569514
4	14.703497671897313	26.099748010524838	14.855331347630196	44.34142296994765
5	16.182711982859097	29.596362268102673	19.087850420197526	35.13307532884071
6	13.518240746031282	35.35410876663215	21.44657699723176	29.681073490104804
7	9.413233454754048	33.72821456985493	33.27907355205148	23.579478423339538
8	8.870897169767584	36.197092764721	23.4675758143526	31.464434251158814
9	9.118824358262852	47.00067159535797	24.48731343334776	19.393190613031418
10-11	9.20726858600933	41.359513191443575	29.57981909645947	19.85339912608762
12-13	8.432920496612168	47.68682160113287	23.70517925552106	20.175078646733905
14-15	8.989161745477446	41.93657249856342	23.080375727621576	25.993890028337564
16-17	9.003464125589028	46.58956674357016	23.46070933495204	20.94625979588878
18-19	14.76268296842135	34.298543013269686	24.524319549092763	26.41445446921621
20-21	19.824165391185367	34.52856362097519	24.48313020604817	21.164140781791275
22-23	7.735411813555521	41.03999301750377	24.318489590874005	26.906105578066708
24-25	7.729684116548076	40.79772676436309	23.930116207013754	27.542472912075077
26-27	7.7550790442882755	40.751540357129215	29.593979887259085	21.899400711323427
28-29	7.814376274748212	40.3466313131889	23.56763304803851	28.271359364024377
30-31	7.707124277163409	35.10589044859448	29.792783092544205	27.394202181697903
32-33	7.764637203316446	41.25343472781292	25.976345855081284	25.005582213789346
34-35	7.843151862551963	40.972842950645585	25.69036566205325	25.493639524749202
36-37	7.859799210549307	40.26523967851844	26.328459283399226	25.546501827533035
38-39	7.827666936413934	40.56694693393732	26.305639576280598	25.299746553368145
40-41	7.832633053713173	40.728634049449965	25.93579661120763	25.502936285629236
42-43	7.7984266010917045	40.49810536325015	26.17517713736881	25.52829089828934
44-45	7.839779643577501	40.32022274250508	26.32411680665819	25.515880807259233
46-47	7.84901160069516	39.962180800811936	26.30563295228667	25.883174646206236
48-49	7.8485536428749905	40.29775753172193	26.32273828616483	25.53095053923825
50-51	7.8310225331726215	40.08117557283054	26.225619045594616	25.862182848402217
52-53	7.8252061605175784	40.49029341276578	26.302330027400302	25.38217039931634
54-55	7.787010800454448	40.2119751047185	26.392829959094538	25.608184135732515
56-57	7.776727225069437	39.88478325209353	26.428338423273207	25.910151099563834
58-59	7.824762362218641	40.14830536793585	26.527949104717845	25.49898316512767
60-61	7.790447268004484	40.358608960294276	26.4117318907586	25.439211880942636
62-63	7.78493673052009	40.28395023646757	26.403084088869438	25.5280289441429
64-65	7.793435771799885	40.1836535974306	26.391228129223364	25.631682501546155
66-67	7.785405479486189	40.066443332251104	26.71397193491104	25.43417925335166
68-69	7.793232525291844	40.128551776306665	26.71527400402727	25.362941694374225
70-71	7.798198661539832	39.99311298582731	26.76414156361085	25.44454678902201
72-73	7.798001795715072	39.973770804237816	26.850504568030185	25.377722832016925
74-75	7.7961180640461025	40.198320947194105	26.750152111899457	25.255408876860336
76-77	7.817606582994969	39.85477425565036	26.869208163451464	25.458410997903208
78-79	7.835399689827819	40.057483816414994	26.813430316691168	25.29368617706602
80-81	7.829106638625662	40.19195824949794	26.90068136606975	25.07825374580665
82-83	7.809047192949114	40.17865289686753	26.614727807755262	25.39757210242809
84-85	7.7865572029840475	40.07094904403628	26.92003860130126	25.222455151678407
86-87	7.876919374995028	40.52493387243908	26.843978803040375	24.754167949525517
88-89	8.002850378220286	40.33960450614998	26.764167134011608	24.89337798161813
90-91	8.101937720778071	40.17283990890817	26.706364000708355	25.0188583696054
92-93	8.50526940890504	40.16815504835325	26.6023047770311	24.724270765710614
94-95	8.66009408518532	40.652296823382706	26.26742870736753	24.420180384064444
96-97	8.956608489790394	40.54823372611838	26.239051021668615	24.25610676242261
98-99	9.184236734615551	40.58705322679679	25.90349842341812	24.32521161516954
100-101	9.335445367882897	41.074421135730326	25.439306485357417	24.15082701102936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4052.0
1	4012.0
2	3717.0
3	3239.5
4	3034.5
5	3100.0
6	3209.0
7	3294.0
8	3398.5
9	3701.0
10	4481.0
11	5612.5
12	7070.5
13	10080.0
14	14241.5
15	18771.0
16	24548.5
17	32127.0
18	42141.0
19	54982.5
20	71488.5
21	93069.0
22	120555.5
23	154670.5
24	195142.5
25	239010.5
26	282680.5
27	325261.0
28	363653.5
29	392920.5
30	411634.0
31	417815.5
32	416450.0
33	407460.5
34	383409.0
35	354016.0
36	326501.5
37	300798.0
38	274665.0
39	247479.5
40	220849.0
41	197010.5
42	179354.0
43	164548.0
44	150437.5
45	137924.0
46	128082.5
47	119192.0
48	110184.0
49	102553.0
50	97132.0
51	91058.5
52	82867.0
53	75278.0
54	68459.0
55	62360.0
56	57343.0
57	52416.5
58	47754.0
59	43648.5
60	39899.5
61	36144.0
62	32795.0
63	29520.0
64	26359.5
65	23696.0
66	21430.0
67	19608.0
68	18059.5
69	16666.0
70	15113.0
71	13850.0
72	12915.0
73	12022.5
74	11298.0
75	10441.5
76	9733.5
77	9295.0
78	8927.5
79	8423.0
80	7964.0
81	7670.0
82	7417.5
83	7004.0
84	6623.0
85	6548.5
86	6485.0
87	6453.5
88	5450.0
89	4438.0
90	3995.5
91	3564.0
92	3184.0
93	2621.5
94	2371.5
95	2293.5
96	2252.0
97	2220.0
98	2203.5
99	2194.5
100	2194.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.330284228522679E-4
3	0.011427910593823488
4	0.010843109907840404
5	3.696979663462572E-4
6	5.480846291995101E-5
7	2.3307019251597903E-4
8	0.0
9	5.488008084933511E-5
10-11	0.0
12-13	0.0
14-15	3.4331072105825394E-5
16-17	6.868222438098085E-6
18-19	3.710093818653912E-4
20-21	6.873743694457144E-5
22-23	0.0011210935049757295
24-25	5.5076667754201366E-5
26-27	4.1376844855589295E-5
28-29	5.597372303206755E-4
30-31	7.618134234433953E-4
32-33	2.638352790259424E-4
34-35	9.747439660911639E-4
36-37	0.0
38-39	6.303593237253032E-5
40-41	6.321688385295388E-5
42-43	0.0035438195399727698
44-45	0.001038952888151209
46-47	0.003962826560125472
48-49	0.0034344300115426714
50-51	0.005463620277534792
52-53	7.154872729481632E-6
54-55	0.0
56-57	0.0
58-59	7.225168846777073E-5
60-61	5.0747209157232394E-5
62-63	2.037004218635737E-4
64-65	1.4601870864704541E-5
66-67	5.863074634448289E-5
68-69	7.358429699328676E-6
70-71	3.0296858969451607E-4
72-73	1.4843071625242128E-5
74-75	0.0
76-77	0.0013427338706719243
78-79	8.29578672829481E-5
80-81	0.0
82-83	4.5812923993839385E-4
84-85	0.0
86-87	0.0
88-89	0.0013474221027404707
90-91	3.883517768064668E-5
92-93	1.0131220345236141E-4
94-95	0.0
96-97	1.5735527327418244E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	13433.0
5-9	16417.0
10-14	5378.0
15-19	6523.0
20-24	14685.0
25-29	36532.0
30-34	47553.0
35-39	52605.0
40-44	54636.0
45-49	53165.0
50-54	55378.0
55-59	57510.0
60-64	60870.0
65-69	68571.0
70-74	79636.0
75-79	92130.0
80-84	114089.0
85-89	45100.0
90-94	60238.0
95-99	127762.0
100-102	6254481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.56356671741538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.21230175504628	84.32341038801727
2	3.28959006146229	5.826756577625243
3	0.687756288040259	1.827304497035263
4	0.277360812918534	0.9825625143882861
5	0.1461338440747508	0.6471067224693284
6	0.08904083812206177	0.47314645245586806
7	0.06028641896387558	0.3737426201642876
8	0.04750172547283955	0.33655377864849406
9	0.03598820836736049	0.286851968350468
>10	0.1360007311787303	2.3516350818440634
>50	0.011582905503613086	0.7100415043836419
>100	0.005564849999030856	0.9095786472402134
>500	5.491498327542524E-4	0.34187775978852875
>1k	3.114223510092106E-4	0.44149982540801863
>5k	3.098866659676037E-5	0.16793166218098907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.833689322989133E-5	0.0	1.3667378645978265E-5
2	6.833689322989133E-5	0.0	1.3667378645978265E-5
3	6.833689322989133E-5	0.0	1.3667378645978265E-5
4	8.200427187586959E-5	0.0	1.3667378645978265E-5
5	8.200427187586959E-5	0.0	1.3667378645978265E-5
6	9.567165052184785E-5	0.0	1.3667378645978265E-5
7	9.567165052184785E-5	0.0	1.3667378645978265E-5
8	9.567165052184785E-5	0.0	1.3667378645978265E-5
9	9.567165052184785E-5	0.0	1.3667378645978265E-5
10-11	9.567165052184785E-5	0.0	1.3667378645978265E-5
12-13	1.0250533984483699E-4	0.0	1.3667378645978265E-5
14-15	1.1617271849081524E-4	0.0	1.3667378645978265E-5
16-17	1.2300640781380437E-4	0.0	1.3667378645978265E-5
18-19	1.3667378645978266E-4	0.0	1.3667378645978265E-5
20-21	1.6400854375173918E-4	0.0	1.3667378645978265E-5
22-23	1.913433010436957E-4	0.0	1.3667378645978265E-5
24-25	2.391791263046196E-4	0.0	1.3667378645978265E-5
26-27	2.4601281562760874E-4	0.0	1.3667378645978265E-5
28-29	2.8018126224255445E-4	0.0	2.733475729195653E-5
30-31	3.143497088575001E-4	0.0	4.1002135937934796E-5
32-33	3.4851815547244576E-4	2.733475729195653E-5	4.1002135937934796E-5
34-35	3.553518447954349E-4	2.733475729195653E-5	4.1002135937934796E-5
36-37	3.8952029141038055E-4	2.733475729195653E-5	4.1002135937934796E-5
38-39	4.5102349531728274E-4	2.733475729195653E-5	4.1002135937934796E-5
40-41	4.7152456328625013E-4	2.733475729195653E-5	4.1002135937934796E-5
42-43	4.7835825260923926E-4	2.733475729195653E-5	4.1002135937934796E-5
44-45	5.056930099011957E-4	2.733475729195653E-5	4.783582526092393E-5
46-47	5.945309711000546E-4	2.733475729195653E-5	5.466951458391306E-5
48-49	6.560341750069567E-4	2.733475729195653E-5	5.466951458391306E-5
50-51	7.24371068236848E-4	2.733475729195653E-5	6.15032039069022E-5
52-53	8.337100974046742E-4	2.733475729195653E-5	6.833689322989133E-5
54-55	8.883796119885872E-4	2.733475729195653E-5	6.833689322989133E-5
56-57	9.635501945414677E-4	2.733475729195653E-5	6.833689322989133E-5
58-59	0.001031887087771359	2.733475729195653E-5	7.517058255288046E-5
60-61	0.0011275587382932068	2.733475729195653E-5	8.200427187586959E-5
62-63	0.0012573988354300004	2.733475729195653E-5	8.200427187586959E-5
64-65	0.001441908447150707	3.4168446614945664E-5	8.200427187586959E-5
66-67	0.0015854159229334787	4.783582526092393E-5	9.567165052184785E-5
68-69	0.001797260291946142	5.466951458391306E-5	9.567165052184785E-5
70-71	0.002159445826064566	5.466951458391306E-5	9.567165052184785E-5
72-73	0.002890650583624403	5.466951458391306E-5	9.567165052184785E-5
74-75	0.004168550487023371	5.466951458391306E-5	9.567165052184785E-5
76-77	0.0059043075750626105	5.466951458391306E-5	9.567165052184785E-5
78-79	0.007749403692269676	5.466951458391306E-5	9.567165052184785E-5
80-81	0.01001135485817908	5.466951458391306E-5	9.567165052184785E-5
82-83	0.013175353014723046	5.466951458391306E-5	9.567165052184785E-5
84-85	0.016332517481944028	5.466951458391306E-5	9.567165052184785E-5
86-87	0.39764554801541463	5.466951458391306E-5	9.567165052184785E-5
88-89	0.9603861963849236	5.466951458391306E-5	9.567165052184785E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	121800	0.0	64.10584	5
TAAATCA	111340	0.0	62.674004	3
ATAAATC	110520	0.0	62.462135	2
ATCATAA	128605	0.0	61.85884	6
TCATAAA	127780	0.0	61.819992	7
AAATCAT	123090	0.0	61.26718	4
CATAAAC	129480	0.0	60.726707	8
AATAAAT	136150	0.0	50.719063	1
ATAAACA	158155	0.0	49.744286	9
AACCGGG	715	0.0	44.67173	1
ACACGGG	980	0.0	39.49675	2
CACACGG	860	0.0	38.70916	1
CACGGGC	990	0.0	38.201378	3
CCAACGG	1065	0.0	37.612255	2
ACCGGGC	840	0.0	37.5066	2
GACCGTT	1135	0.0	34.896008	2
AGAGCAC	5515	0.0	34.44323	94-95
AAACGGG	116105	0.0	34.25892	14-15
AGAAATC	2735	0.0	33.899937	2
ACAAACG	119585	0.0	33.741352	12-13
>>END_MODULE