FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_TTAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_TTAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13428815
Sequences flagged as poor quality0
Sequence length1-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA1316750.054.2826045
TCATAAA1416100.051.962737
ATCATAA1437100.051.597566
TAAATCA1303300.051.2348183
AAATCAT1427350.049.6641584
AGAGCAC170350.048.86280494-95
CATAAAC1517150.048.4539958
ATAAATC1449700.046.15822
GAGCACA96950.041.9792894-95
AATAAAT1653400.040.3541871
AACCGGG9650.040.206651
CACGGGC14300.039.0011863
CACACGG13750.038.272361
ATAAACA1944800.038.013249
AGCCCGA8150.037.7569961
CCGGGCA11400.036.398373
AGCCCAC14200.035.803251
AGCCCCC13100.035.7457121
ACCGGGC11200.035.4493032
AGCCGCC11600.034.2166861
Produced by FastQC (version 0.11.2)