##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 20150414_trimmed_2112_lane1_TTAGGC_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13428815 Sequences flagged as poor quality 0 Sequence length 1-101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.688228931592253 31.0 28.0 31.0 16.0 34.0 2 30.20599362385511 31.0 30.0 33.0 26.0 34.0 3 27.169922811685016 31.0 25.0 31.0 16.0 34.0 4 31.641558048153005 35.0 30.0 37.0 19.0 37.0 5 32.70737267723238 35.0 32.0 37.0 25.0 37.0 6 34.15932520527613 35.0 33.0 37.0 30.0 37.0 7 34.8283058217154 35.0 35.0 37.0 32.0 37.0 8 35.00478417368011 35.0 35.0 37.0 32.0 37.0 9 36.651546714903255 39.0 35.0 39.0 32.0 39.0 10-11 36.79166522064849 39.0 36.0 39.0 32.5 39.0 12-13 36.79111160879363 39.0 36.5 39.0 32.5 39.0 14-15 37.90923336964799 40.0 37.0 41.0 33.0 41.0 16-17 37.87411171503719 40.0 37.0 41.0 33.0 41.0 18-19 37.98590153433567 40.0 37.0 41.0 33.0 41.0 20-21 37.94910318763034 40.0 37.0 41.0 32.5 41.0 22-23 38.096134277684286 40.0 37.0 41.0 33.0 41.0 24-25 37.97325597504046 40.0 37.0 41.0 33.0 41.0 26-27 37.713966703642086 40.0 37.0 41.0 32.0 41.0 28-29 37.63994552542496 40.0 37.0 41.0 32.0 41.0 30-31 37.67229142672264 40.0 37.0 41.0 32.5 41.0 32-33 37.458151234682546 39.0 37.0 41.0 31.5 41.0 34-35 37.534158015641054 39.5 37.0 41.0 32.0 41.0 36-37 37.402458483351964 39.5 36.5 41.0 31.5 41.0 38-39 37.44956713567471 39.0 36.0 41.0 31.5 41.0 40-41 37.560122905617845 39.5 37.0 41.0 32.0 41.0 42-43 37.41679640811407 39.0 36.0 41.0 31.5 41.0 44-45 37.28347367553069 39.0 36.0 40.5 31.0 41.0 46-47 37.37190615805458 39.0 36.0 40.0 31.5 41.0 48-49 37.268192822265725 39.0 36.0 40.0 31.0 41.0 50-51 37.06331686973975 39.0 35.5 40.0 31.0 41.0 52-53 36.93164756126566 39.0 35.0 40.0 31.0 41.0 54-55 36.80009894743754 38.5 35.0 40.0 31.0 41.0 56-57 36.57372754907 38.0 35.0 40.0 31.0 41.0 58-59 36.32114455104397 38.0 34.5 40.0 30.5 41.0 60-61 36.11688125498078 37.0 34.0 40.0 30.0 41.0 62-63 35.859582895643626 37.0 34.0 39.5 30.0 41.0 64-65 35.83323446544025 36.5 34.0 39.0 30.0 41.0 66-67 35.5751182363187 36.0 34.0 39.0 30.0 40.5 68-69 35.307136350056325 36.0 34.0 39.0 30.0 40.0 70-71 35.070680991995815 35.0 34.0 37.5 30.0 40.0 72-73 34.82677155180785 35.0 34.0 37.0 30.0 39.0 74-75 34.528494735216405 35.0 33.5 37.0 30.0 39.0 76-77 31.733513087406177 32.5 30.0 34.5 26.5 36.5 78-79 33.82541750872668 35.0 33.0 36.0 29.5 37.0 80-81 33.97192953891026 35.0 33.0 35.5 30.0 37.0 82-83 33.86334633428498 35.0 33.0 35.0 30.0 37.0 84-85 33.74624248390313 35.0 33.0 35.0 30.0 36.0 86-87 33.63840214345537 35.0 33.0 35.0 30.0 36.0 88-89 33.51921853047946 35.0 33.0 35.0 30.0 36.0 90-91 33.462279574122086 35.0 33.0 35.0 30.0 36.0 92-93 33.391449025058705 35.0 33.0 35.0 30.0 35.5 94-95 33.337050731606205 35.0 33.0 35.0 30.0 35.0 96-97 33.33782200714349 35.0 33.0 35.0 30.0 35.0 98-99 33.386471110784186 35.0 33.0 35.0 30.0 35.0 100-101 32.522769205809496 34.0 31.5 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.5287733683421862 1101 2 1.4783666972493386 1101 3 3.037368822044268 1101 4 2.6554393581305256 1101 5 2.8675525874226935 1101 6 1.420601383660184 1101 7 0.6896176426079421 1101 8 0.46374974039812145 1101 9 0.5616601285539389 1101 10-11 0.5082377135509333 1101 12-13 0.45345716477027764 1101 14-15 0.4525932350959607 1101 16-17 0.45767036193907273 1101 18-19 0.3070463692266969 1101 20-21 0.5042583648128485 1101 22-23 0.29542039925334507 1101 24-25 0.38705365065273867 1101 26-27 0.3815142010556869 1101 28-29 0.5923286205129585 1101 30-31 0.5293928512608375 1101 32-33 0.6138329021016062 1101 34-35 0.5238769700219592 1101 36-37 0.5871380138084987 1101 38-39 0.5447124388152247 1101 40-41 0.4816878069698589 1101 42-43 0.36920484528202024 1101 44-45 0.3562713469585006 1101 46-47 0.3855741684922762 1101 48-49 0.3436324793615171 1101 50-51 0.25405344559927556 1101 52-53 0.29989004775872274 1101 54-55 0.1913258800000719 1101 56-57 0.16578341472573044 1101 58-59 0.1520636380554592 1101 60-61 0.15829158477981053 1101 62-63 0.28967042226545203 1101 64-65 0.21430970080042755 1101 66-67 0.23865466701521854 1101 68-69 0.22413794993168068 1101 70-71 0.22648823055587286 1101 72-73 0.13865792970975122 1101 74-75 0.13837698755177996 1101 76-77 -0.828622703238878 1101 78-79 -0.07119057414215035 1101 80-81 0.09642396710251688 1101 82-83 0.05026627513390736 1101 84-85 0.025382276241565194 1101 86-87 0.07459828854253914 1101 88-89 0.03909781967874437 1101 90-91 -0.00905782940786537 1101 92-93 0.01330923495540759 1101 94-95 0.01073302907870044 1101 96-97 0.017523376288387738 1101 98-99 0.045705589125851986 1101 100-101 -0.35538478763628234 1102 1 2.800866570359375 1102 2 1.7132415287361908 1102 3 3.8982060492844752 1102 4 3.1487812898279834 1102 5 3.1024341189594082 1102 6 1.6110016693419098 1102 7 0.8137528779393648 1102 8 0.5694817299075225 1102 9 0.6242481407951672 1102 10-11 0.5976648854346323 1102 12-13 0.5332694492665198 1102 14-15 0.4568161317293118 1102 16-17 0.5511434436832943 1102 18-19 0.5018731979842244 1102 20-21 0.7443508955450611 1102 22-23 0.5533028804392686 1102 24-25 0.5264171103508062 1102 26-27 0.5625544575296573 1102 28-29 0.636359614717513 1102 30-31 0.64627924575381 1102 32-33 0.7443458335086035 1102 34-35 0.78115159605359 1102 36-37 0.9508484596716187 1102 38-39 0.8528765163800784 1102 40-41 0.7233090317858455 1102 42-43 0.5637840625250163 1102 44-45 0.6650238460658358 1102 46-47 0.5120602648594215 1102 48-49 0.557690320374256 1102 50-51 0.6205020625198969 1102 52-53 0.640201474713777 1102 54-55 0.5566322712846414 1102 56-57 0.4944384694366377 1102 58-59 0.47163461418841024 1102 60-61 0.4353037554020034 1102 62-63 0.47620433584399535 1102 64-65 0.3001401171179623 1102 66-67 0.380928598817043 1102 68-69 0.40314006630003263 1102 70-71 0.3123538241534334 1102 72-73 0.22897819973657363 1102 74-75 0.19536982190477659 1102 76-77 0.008877500693298401 1102 78-79 0.1665433621180128 1102 80-81 0.2503876871042223 1102 82-83 0.2270240348638879 1102 84-85 0.2681673152658064 1102 86-87 0.2913841843135003 1102 88-89 0.13887443383722342 1102 90-91 0.19036678178193256 1102 92-93 0.1282048257404611 1102 94-95 0.0832076904508483 1102 96-97 0.20875214027788758 1102 98-99 0.21587363380107405 1102 100-101 -0.03429435669045944 1103 1 1.5091400604901537 1103 2 0.7256045036630958 1103 3 1.7854698982091506 1103 4 -0.5502459995092224 1103 5 0.68989921985861 1103 6 0.40021457525671167 1103 7 0.20940889321075673 1103 8 0.1929501534265725 1103 9 0.2864259768774744 1103 10-11 0.3250003185929913 1103 12-13 0.25456154764858496 1103 14-15 0.27157290289528646 1103 16-17 0.23083936118971593 1103 18-19 0.2805059128318135 1103 20-21 0.3152102929881977 1103 22-23 0.30931115841003987 1103 24-25 0.31666739148123924 1103 26-27 0.3341054512102204 1103 28-29 0.4034330766658343 1103 30-31 0.3817943071868726 1103 32-33 0.41985325705459786 1103 34-35 0.3701525360747553 1103 36-37 0.481130307879738 1103 38-39 0.37348419200206706 1103 40-41 0.35296363922204677 1103 42-43 0.24745147104302845 1103 44-45 0.19976889037423717 1103 46-47 0.21813711858176532 1103 48-49 0.20319669936408502 1103 50-51 0.143228887316873 1103 52-53 0.1969093400173847 1103 54-55 0.18298729104475342 1103 56-57 0.17042612702703508 1103 58-59 0.1128448645004525 1103 60-61 0.06702970554051291 1103 62-63 0.1735182381949727 1103 64-65 0.12541725432121353 1103 66-67 0.10941027228748368 1103 68-69 0.15370829572319877 1103 70-71 -0.013100508602846617 1103 72-73 -0.06694908826634816 1103 74-75 -0.05392465204131014 1103 76-77 -0.5519481961643891 1103 78-79 -0.19303690609091717 1103 80-81 -0.06046682742585574 1103 82-83 -0.06077616517297457 1103 84-85 -0.13224331344923002 1103 86-87 -0.012587286464544434 1103 88-89 -0.12699496830408208 1103 90-91 -0.046670514969960664 1103 92-93 -0.0942840777839109 1103 94-95 -0.21201709948965686 1103 96-97 -0.0584438021633531 1103 98-99 -0.08385102844606962 1103 100-101 -0.27952869420458626 1216 1 0.9533286787585133 1216 2 0.2913332371534594 1216 3 0.976765262325852 1216 4 -0.4034516989742265 1216 5 1.1777618860628252 1216 6 0.5548489202973528 1216 7 0.25563580298369004 1216 8 0.19566191352419082 1216 9 0.20689286179766242 1216 10-11 0.24953583997552187 1216 12-13 0.18734936644678868 1216 14-15 0.18632594792617851 1216 16-17 0.181735863421693 1216 18-19 0.17094979679935562 1216 20-21 0.26206638766018386 1216 22-23 0.1641048558492244 1216 24-25 0.22883631903038548 1216 26-27 0.19623821747263293 1216 28-29 0.31055054426311557 1216 30-31 0.2762897367407433 1216 32-33 0.29357084678465384 1216 34-35 0.24061908057351644 1216 36-37 0.3843879633526939 1216 38-39 0.29022385810243634 1216 40-41 0.19269778377520197 1216 42-43 0.11922745406894109 1216 44-45 0.1300073050945727 1216 46-47 0.1706296901941471 1216 48-49 0.24366637486897957 1216 50-51 0.20120604816937515 1216 52-53 0.2250867058720729 1216 54-55 0.1445955030586603 1216 56-57 0.11325217274146127 1216 58-59 0.14376604618023237 1216 60-61 0.11071440202248795 1216 62-63 0.13862057268495676 1216 64-65 0.02980305564804553 1216 66-67 0.0619378891838096 1216 68-69 -0.02007116362194239 1216 70-71 -0.03587493971225797 1216 72-73 -0.08985073088249607 1216 74-75 -0.15686616917779617 1216 76-77 -0.5570659793593151 1216 78-79 -0.1362165751253741 1216 80-81 0.017858111183514325 1216 82-83 -0.029071798581576047 1216 84-85 0.008317772797418854 1216 86-87 0.01122170396030242 1216 88-89 -0.03960974622989255 1216 90-91 -0.07098301287653896 1216 92-93 -0.042222343337918744 1216 94-95 -0.11600462665131062 1216 96-97 -0.06650450537701857 1216 98-99 -0.05039681739547319 1216 100-101 -0.25047410470340736 1113 1 2.427090737916224 1113 2 1.2809349470228 1113 3 2.4832377204362857 1113 4 3.3811645490521833 1113 5 2.8917270744377817 1113 6 1.1810142410454887 1113 7 0.5277906492843982 1113 8 0.3368300409483993 1113 9 0.1689793965226727 1113 10-11 0.1118293759796174 1113 12-13 0.07996243837421702 1113 14-15 0.11465713347052997 1113 16-17 0.05395252428333919 1113 18-19 0.11099363927263539 1113 20-21 0.1446717695234625 1113 22-23 0.05742992593521734 1113 24-25 0.001867691236654423 1113 26-27 0.056728932427766665 1113 28-29 0.0415454572427123 1113 30-31 0.23139368425777462 1113 32-33 0.3432008883151525 1113 34-35 0.13459297315188223 1113 36-37 0.024562842703794274 1113 38-39 0.017254707730280927 1113 40-41 0.046102773324520285 1113 42-43 -0.05953674524802466 1113 44-45 0.12267625354984091 1113 46-47 0.04705231529840148 1113 48-49 -0.03077450284902028 1113 50-51 -0.0983489778302058 1113 52-53 0.027449433194277617 1113 54-55 -0.09059842666818696 1113 56-57 0.018851283110343786 1113 58-59 -0.02047390842144381 1113 60-61 -0.20514129319075636 1113 62-63 -0.020335084682272964 1113 64-65 -0.17806278040986 1113 66-67 -0.03100901187847427 1113 68-69 -0.09694540889336167 1113 70-71 -0.12159494386557412 1113 72-73 -0.07664152655399192 1113 74-75 -0.07248749469841442 1113 76-77 -0.5836617795695247 1113 78-79 -0.15474800545765532 1113 80-81 -0.0672515773458997 1113 82-83 0.0037150391133309313 1113 84-85 0.023699233296362365 1113 86-87 -0.004628191037589602 1113 88-89 0.010766734710237813 1113 90-91 -0.05770094784755031 1113 92-93 -0.020516447908697444 1113 94-95 -0.01056138661521544 1113 96-97 -0.0730282467653538 1113 98-99 -0.06793154407016999 1113 100-101 -0.24271560402689119 1112 1 2.1085831536505424 1112 2 1.08212624675771 1112 3 1.6998269777942596 1112 4 3.127548683166676 1112 5 2.3292226121431483 1112 6 0.9923236064702508 1112 7 0.4643425958605931 1112 8 0.271763641967361 1112 9 0.14348763243901175 1112 10-11 0.11062133009266262 1112 12-13 0.027745581091600968 1112 14-15 -0.05018785430856809 1112 16-17 -0.071083736886699 1112 18-19 -7.310355574006167E-4 1112 20-21 0.11778715430116193 1112 22-23 -0.011078432569391339 1112 24-25 0.05132291888175189 1112 26-27 0.013867730064660577 1112 28-29 -0.0770850620207284 1112 30-31 0.04401492803774687 1112 32-33 0.14308593078968102 1112 34-35 0.02000191955254138 1112 36-37 0.08920944367481809 1112 38-39 0.045860892463551295 1112 40-41 0.049358638810872435 1112 42-43 -0.05738519765653649 1112 44-45 -0.006479972581551863 1112 46-47 -0.0621752520022838 1112 48-49 -0.10399970184164431 1112 50-51 -0.10081922205066718 1112 52-53 -0.09222122013466105 1112 54-55 -0.064018584841385 1112 56-57 -0.1568358121878788 1112 58-59 -0.22906665264418535 1112 60-61 -0.1806340290480719 1112 62-63 -0.207293239685292 1112 64-65 -0.07736107505794365 1112 66-67 -0.0665503987281042 1112 68-69 -0.03273673155623413 1112 70-71 -0.06537960705406931 1112 72-73 -0.1495005967920875 1112 74-75 -0.10123537784480874 1112 76-77 -0.6584584934373616 1112 78-79 -0.16009244180401794 1112 80-81 -0.08295880973923886 1112 82-83 -0.0959083893921786 1112 84-85 -0.04162724750245417 1112 86-87 -0.10856848052372214 1112 88-89 -0.09178258432506681 1112 90-91 -0.027713512562307585 1112 92-93 -0.036116436912926986 1112 94-95 -0.05353459999059851 1112 96-97 -0.0585175108300362 1112 98-99 -0.09293661934440678 1112 100-101 -0.17960635488784504 1115 1 1.4703267927162678 1115 2 0.7826092902255404 1115 3 0.015699456659191924 1115 4 0.1129310528177001 1115 5 1.6668179162450372 1115 6 0.6756544613849229 1115 7 0.24526245255657386 1115 8 0.12035858995394477 1115 9 0.027926214537764338 1115 10-11 0.07550467045859222 1115 12-13 -0.08812225917717598 1115 14-15 -0.15080169458805415 1115 16-17 -0.13819391292133787 1115 18-19 -0.037590301127586656 1115 20-21 -0.04385323115445772 1115 22-23 -0.17912952474376453 1115 24-25 -0.12331937660326986 1115 26-27 -0.19479320780983045 1115 28-29 -0.1881244414898262 1115 30-31 -0.009103478242103336 1115 32-33 0.0378250087770482 1115 34-35 -0.1230096862662009 1115 36-37 0.061216730701630695 1115 38-39 0.1071522531875928 1115 40-41 -0.03484298535175867 1115 42-43 -0.15067701762178132 1115 44-45 -0.1322752280333006 1115 46-47 -0.0646202683622974 1115 48-49 -0.1069672379660247 1115 50-51 -0.12493546874568295 1115 52-53 -0.07227080035244171 1115 54-55 -0.1739094166863211 1115 56-57 -0.1876206635840063 1115 58-59 -0.13103097336414038 1115 60-61 -0.16481108607106165 1115 62-63 -0.026373572192802897 1115 64-65 -0.18161041106487374 1115 66-67 -0.09580316352575124 1115 68-69 -0.08353356461410044 1115 70-71 -0.1445625501936192 1115 72-73 -0.26881498156871686 1115 74-75 -0.20088344132681613 1115 76-77 -0.3816417653399 1115 78-79 -0.14833982653617284 1115 80-81 -0.07516921454903525 1115 82-83 -0.11891906809974273 1115 84-85 -0.081853585071606 1115 86-87 -0.11272748115495546 1115 88-89 -0.05260657304911831 1115 90-91 -0.027861686067687685 1115 92-93 -0.098475590280664 1115 94-95 -0.08040304767781947 1115 96-97 -0.06023008514512185 1115 98-99 -0.06376223799987457 1115 100-101 -0.05081635355882952 1114 1 1.9793954635393263 1114 2 0.9594878914273757 1114 3 3.4855862645618565 1114 4 0.6190747169306796 1114 5 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-0.21919867810531457 1213 66-67 -0.27886394886169086 1213 68-69 -0.1616499459804075 1213 70-71 -0.34117680000753126 1213 72-73 -0.21656256036038712 1213 74-75 -0.09259659650088992 1213 76-77 0.6514686625964643 1213 78-79 0.2587825139706652 1213 80-81 0.093356295630322 1213 82-83 0.10063350212202238 1213 84-85 0.10131303378359036 1213 86-87 0.1749106300598129 1213 88-89 0.09737098450305126 1213 90-91 0.06463157372765238 1213 92-93 -5.98111370301524E-4 1213 94-95 0.03601468372633576 1213 96-97 0.05799742470561853 1213 98-99 0.1272610488685757 1213 100-101 0.39760708192712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 213.0 15 6971.0 16 6653.0 17 6587.0 18 5383.0 19 3909.0 20 4747.0 21 3526.0 22 4340.0 23 5960.0 24 11723.0 25 25969.0 26 57807.0 27 115890.0 28 203542.0 29 314138.0 30 438587.0 31 570903.0 32 721492.0 33 907717.0 34 1166931.0 35 1562171.0 36 2232583.0 37 2974162.0 38 1858265.0 39 215425.0 40 3221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.672748861310549 25.991980677371757 13.418287466168833 47.916982995148864 2 16.44644062371608 26.002977190289883 24.521278033663297 33.029304152330745 3 11.00901783016287 17.750436710023727 30.54610557742737 40.694439882386035 4 15.749494440710599 19.173423601310805 13.99152657297117 51.08555538500743 5 19.811203239959823 23.267206132866338 17.885261766120024 39.03632886105382 6 15.948197500982872 31.398563290532756 21.239705219027442 31.413533989456926 7 9.105684379659488 37.771016555206046 27.147999345945045 25.975299719189422 8 8.090141061351746 40.47888531163739 23.72246287715684 27.70851074985402 9 8.174497978932248 45.41201671178638 24.877566450506905 21.53591885877447 10-11 8.50890287372645 41.77387499533909 27.039121503320473 22.678100627613983 12-13 8.080582406781959 44.94580243468781 24.27247550613284 22.701139652397387 14-15 8.519778334573743 41.737529917792735 23.710018278458637 26.03267346917488 16-17 8.584589229491392 43.471789503838274 23.86933070355241 24.07429056311792 18-19 11.594407348720468 37.08636166608177 24.722197313837384 26.597033671360382 20-21 14.24561767511289 37.2795710912644 24.68842699319993 23.78638424042278 22-23 7.99509133117238 40.2425061230117 24.61130001055527 27.15110253526065 24-25 7.985770494570159 39.97792748604701 24.644088113481697 27.392213905901137 26-27 8.153985462580211 40.168432740744095 27.437853410428815 24.23972838624688 28-29 8.2250150976258 39.77188533526617 24.136967150055117 27.866132417052913 30-31 8.04326368913948 36.920839534217706 27.531628984492475 27.504267792150337 32-33 8.153939160636208 40.13759478909092 25.5750307786465 26.13343527162637 34-35 8.310652677223839 39.78440701988904 25.24740296009452 26.657537342792605 36-37 8.380992206212811 39.52715045079997 25.44117666607607 26.650680676911144 38-39 8.210392214551158 39.82405997712963 25.64872361028196 26.31682419803726 40-41 8.255320845606597 39.721483569953485 25.509253779863382 26.513941804576536 42-43 8.168524319196115 39.43285392792923 25.681945351828855 26.716676401045795 44-45 8.297055991445527 39.43934815638889 25.72027836006523 26.54331749210036 46-47 8.340130486848336 39.12183602845416 25.62850096442243 26.90953252027507 48-49 8.225457959377827 39.52045961945496 25.68200793403981 26.572074487127406 50-51 8.262144045966833 39.53788325428814 25.681211765145346 26.518760934599676 52-53 8.236344515985515 39.47962400061583 25.6946390845365 26.589392398862156 54-55 8.099254720720404 39.533373555374624 25.944766936701054 26.422604787203923 56-57 8.15181800743274 39.390262277665904 25.86979050430115 26.588129210600204 58-59 8.146596431143829 39.46216814464718 25.90624445799098 26.48499096621802 60-61 8.083721293491749 39.64375881816623 25.903171920032914 26.36934796830911 62-63 8.062251846652977 39.70473770562381 25.87703875539122 26.35597169233199 64-65 8.125578155747961 39.52411048321936 25.772239303068556 26.578072057964125 66-67 8.048672277836518 39.565812429465325 26.005787994112794 26.379727298585365 68-69 8.027019922849062 39.75028613340504 26.052786912214813 26.169907031531086 70-71 7.989474527963565 39.61629902491857 26.041544393551852 26.352682053566014 72-73 7.933634892936321 39.610809794532734 26.20786253593794 26.247692776593002 74-75 7.911743367394624 39.883294462695766 26.094184220048707 26.110777949860903 76-77 7.946814569171791 39.56740971715943 26.03570533023496 26.45007038343382 78-79 7.85701643355888 39.80604361663882 26.107943637519636 26.22899631228266 80-81 7.948455184929489 39.91888691488352 26.15337759314607 25.97928030704092 82-83 7.8361045458351 39.74346592815274 26.048859078760543 26.371570447251614 84-85 7.752575988113055 39.74765444054161 26.219460815634438 26.2803087557109 86-87 7.9748582710150115 40.317773500363764 26.004869421016075 25.70249880760515 88-89 8.259697017106543 40.28058067394856 25.80158202027416 25.65814028867073 90-91 8.426208711632752 40.09132425314996 25.83780557796102 25.644661457256262 92-93 9.409485977602952 39.8765619985276 25.55360565353292 25.160346370336534 94-95 9.780472900337463 40.517694085338526 24.914722071220282 24.787110943103727 96-97 10.420521247031605 40.67018433036195 24.592349709292492 24.316944713313955 98-99 10.867059217018362 40.383342168784736 24.121387008835992 24.62821160536091 100-101 11.018588444588351 40.623013686533405 23.462152995924118 24.896244872954128 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4294.0 1 4274.0 2 4042.5 3 3545.5 4 3275.5 5 3332.5 6 3431.5 7 3492.0 8 3517.0 9 3674.5 10 4169.0 11 4746.0 12 5267.0 13 7153.0 14 9742.0 15 12063.5 16 15171.0 17 19811.0 18 26057.5 19 33689.5 20 44117.0 21 58953.5 22 80000.5 23 111321.0 24 155483.5 25 213734.5 26 288974.5 27 380935.0 28 483760.0 29 586048.5 30 679561.0 31 758294.5 32 817277.0 33 852038.5 34 860475.0 35 850145.5 36 822193.5 37 778455.5 38 728103.0 39 672891.5 40 613026.0 41 554233.0 42 497007.0 43 440274.0 44 388419.5 45 341997.0 46 299740.5 47 262783.5 48 229209.0 49 202068.5 50 185110.0 51 165422.5 52 137182.5 53 111817.5 54 93691.5 55 80105.5 56 69928.5 57 61875.5 58 55538.5 59 50488.5 60 45927.5 61 41826.0 62 38644.5 63 35455.5 64 32294.0 65 29932.0 66 27895.5 67 26209.5 68 24738.0 69 23367.5 70 21669.5 71 20200.0 72 19211.0 73 18259.0 74 17455.5 75 16400.0 76 15475.0 77 15009.0 78 14614.0 79 13956.0 80 13356.0 81 12990.5 82 12670.5 83 12023.5 84 11396.5 85 11264.5 86 11139.0 87 11068.5 88 9425.0 89 7786.0 90 7219.5 91 6651.5 92 6147.0 93 5412.5 94 5027.5 95 4826.5 96 4705.5 97 4631.0 98 4606.5 99 4603.5 100 4604.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.005264802435123783 3 0.0022195318054066453 4 0.013477280724307358 5 3.729496347927996E-4 6 2.9851833410052544E-5 7 1.4932759278245986E-5 8 8.215738112741886E-5 9 7.470356504835338E-6 10-11 0.0 12-13 7.474649352986877E-6 14-15 4.486335016095288E-5 16-17 1.0845216730250179E-4 18-19 3.5912836403675095E-4 20-21 7.48489968269639E-6 22-23 0.0 24-25 0.0 26-27 3.7527723136370454E-5 28-29 2.2557612989485636E-5 30-31 0.0 32-33 2.266190172485022E-5 34-35 1.3634128465606093E-4 36-37 7.216288576189804E-5 38-39 0.0 40-41 1.6804159701692557E-4 42-43 0.0016891875716396514 44-45 0.002678196513994864 46-47 5.81949087779389E-4 48-49 0.003862023769267932 50-51 0.0014468382743571537 52-53 3.89172065618302E-6 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.964348298602801E-6 64-65 8.361029908478972E-5 66-67 3.640486223339989E-4 68-69 4.424482253120125E-5 70-71 4.0462663037219744E-6 72-73 0.0 74-75 7.802044554519887E-5 76-77 2.7778492970320667E-4 78-79 0.0 80-81 0.0 82-83 3.2134781672222917E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 3.092354674350314E-5 94-95 0.0 96-97 3.5748299263491196E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 22179.0 5-9 23023.0 10-14 10687.0 15-19 11280.0 20-24 22236.0 25-29 61248.0 30-34 85296.0 35-39 91757.0 40-44 98540.0 45-49 101118.0 50-54 107893.0 55-59 117991.0 60-64 131862.0 65-69 167756.0 70-74 227359.0 75-79 299085.0 80-84 387321.0 85-89 88010.0 90-94 126967.0 95-99 282286.0 100-102 1.0964921E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.46258081538008 #Duplication Level Percentage of deduplicated Percentage of total 1 84.39766952036578 61.156729482492445 2 10.04134830915529 14.552440266950907 3 2.4652251800606577 5.359097365147659 4 1.083877347709876 3.141621996095467 5 0.5128448962697953 1.8581032370852628 6 0.3615091040607377 1.571752960109811 7 0.23369836104686378 1.1854070461646167 8 0.17009727256305285 0.9860549887660759 9 0.15124288698396796 0.9863504928744457 >10 0.5563965094410259 6.702311118586306 >50 0.017221347346493474 0.8651844864591234 >100 0.00806357153631399 1.020534885965833 >500 5.785265060468726E-4 0.27211341986610427 >1k 2.059609446836617E-4 0.2420849925355756 >5k 2.120600939779665E-5 0.10021326090045998 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.446673440657273E-5 0.0 1.4893346881314547E-5 2 7.446673440657273E-5 0.0 1.4893346881314547E-5 3 7.446673440657273E-5 0.0 1.4893346881314547E-5 4 8.191340784723E-5 0.0 2.234002032197182E-5 5 8.191340784723E-5 0.0 2.234002032197182E-5 6 8.191340784723E-5 0.0 2.234002032197182E-5 7 8.191340784723E-5 0.0 2.234002032197182E-5 8 8.191340784723E-5 0.0 2.234002032197182E-5 9 8.191340784723E-5 0.0 4.468004064394364E-5 10-11 8.936008128788728E-5 0.0 4.468004064394364E-5 12-13 9.308341800821592E-5 0.0 4.468004064394364E-5 14-15 1.0053009144887319E-4 0.0 4.468004064394364E-5 16-17 1.0425342816920183E-4 0.0 4.468004064394364E-5 18-19 1.117001016098591E-4 0.0 4.468004064394364E-5 20-21 1.117001016098591E-4 0.0 4.468004064394364E-5 22-23 1.2659344849117366E-4 0.0 4.468004064394364E-5 24-25 1.6755015241478864E-4 2.234002032197182E-5 4.468004064394364E-5 26-27 1.8616683601643184E-4 2.234002032197182E-5 4.468004064394364E-5 28-29 2.159535297790609E-4 2.9786693762629094E-5 4.468004064394364E-5 30-31 2.4574022354169E-4 3.723336720328637E-5 4.468004064394364E-5 32-33 2.7925025402464776E-4 3.723336720328637E-5 4.468004064394364E-5 34-35 2.941436009059623E-4 3.723336720328637E-5 4.468004064394364E-5 36-37 3.090369477872769E-4 3.723336720328637E-5 4.468004064394364E-5 38-39 3.3137696810924867E-4 4.468004064394364E-5 4.468004064394364E-5 40-41 3.574403251515491E-4 4.468004064394364E-5 4.468004064394364E-5 42-43 3.9467369235483547E-4 4.468004064394364E-5 5.2126714084600914E-5 44-45 4.356303962784505E-4 4.468004064394364E-5 5.2126714084600914E-5 46-47 5.100971306850233E-4 4.468004064394364E-5 5.2126714084600914E-5 48-49 5.473304978883095E-4 4.468004064394364E-5 5.585005080492955E-5 50-51 6.180738955745537E-4 6.329672424558683E-5 5.957338752525819E-5 52-53 7.297739971844127E-4 6.702006096591545E-5 6.329672424558683E-5 54-55 7.930707214299996E-4 6.702006096591545E-5 7.819007112690136E-5 56-57 8.60090782395915E-4 6.702006096591545E-5 8.191340784723E-5 58-59 9.978542410480746E-4 6.702006096591545E-5 8.191340784723E-5 60-61 0.001187744413784835 6.702006096591545E-5 8.191340784723E-5 62-63 0.0013031678521150227 6.702006096591545E-5 8.191340784723E-5 64-65 0.0014521013209281683 6.702006096591545E-5 8.563674456755864E-5 66-67 0.0016122047999022997 6.702006096591545E-5 8.936008128788728E-5 68-69 0.001824434992961032 6.702006096591545E-5 8.936008128788728E-5 70-71 0.002364318817408684 6.702006096591545E-5 9.680675472854455E-5 72-73 0.0038685468524214534 6.702006096591545E-5 9.680675472854455E-5 74-75 0.006471159219931171 6.702006096591545E-5 9.680675472854455E-5 76-77 0.00999343575736206 6.702006096591545E-5 9.680675472854455E-5 78-79 0.014133786190367505 6.702006096591545E-5 9.680675472854455E-5 80-81 0.019182630783133134 7.07433976862441E-5 9.680675472854455E-5 82-83 0.0253410297185567 7.446673440657273E-5 9.680675472854455E-5 84-85 0.032623876343519514 7.446673440657273E-5 9.680675472854455E-5 86-87 0.8881684646039133 7.446673440657273E-5 9.680675472854455E-5 88-89 2.1208200425726322 7.446673440657273E-5 9.680675472854455E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCATA 131675 0.0 54.282604 5 TCATAAA 141610 0.0 51.96273 7 ATCATAA 143710 0.0 51.59756 6 TAAATCA 130330 0.0 51.234818 3 AAATCAT 142735 0.0 49.664158 4 AGAGCAC 17035 0.0 48.862804 94-95 CATAAAC 151715 0.0 48.453995 8 ATAAATC 144970 0.0 46.1582 2 GAGCACA 9695 0.0 41.97928 94-95 AATAAAT 165340 0.0 40.354187 1 AACCGGG 965 0.0 40.20665 1 CACGGGC 1430 0.0 39.001186 3 CACACGG 1375 0.0 38.27236 1 ATAAACA 194480 0.0 38.01324 9 AGCCCGA 815 0.0 37.756996 1 CCGGGCA 1140 0.0 36.39837 3 AGCCCAC 1420 0.0 35.80325 1 AGCCCCC 1310 0.0 35.745712 1 ACCGGGC 1120 0.0 35.449303 2 AGCCGCC 1160 0.0 34.216686 1 >>END_MODULE