##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2112_lane1_TTAGGC_L001_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8034025
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.794426206042427	30.0	25.0	31.0	16.0	34.0
2	29.702762552934285	31.0	30.0	33.0	25.0	34.0
3	26.31094771361847	30.0	23.0	31.0	16.0	34.0
4	30.42813772000903	35.0	28.0	35.0	19.0	37.0
5	32.51525120433775	35.0	32.0	37.0	25.0	37.0
6	34.12686897324748	35.0	33.0	37.0	30.0	37.0
7	34.86068660959379	35.0	35.0	37.0	32.0	37.0
8	35.063035478138815	35.0	35.0	37.0	32.0	37.0
9	36.82393119371191	39.0	37.0	39.0	32.0	39.0
10-11	37.039342908931104	39.0	37.0	39.0	33.0	39.0
12-13	37.01720963577298	39.0	37.0	39.0	33.0	39.0
14-15	38.18376472219617	40.0	37.0	41.0	33.0	41.0
16-17	38.14759007148134	40.0	37.0	41.0	33.0	41.0
18-19	38.25283054922049	40.0	37.5	41.0	33.5	41.0
20-21	38.20327392019864	40.0	37.0	41.0	33.0	41.0
22-23	38.30730042447037	40.0	38.0	41.0	34.0	41.0
24-25	38.202716578503	40.0	37.5	41.0	33.5	41.0
26-27	38.00527180193052	40.0	37.0	41.0	33.0	41.0
28-29	37.96985234217688	40.0	37.0	41.0	33.0	41.0
30-31	38.0165788543361	40.0	37.0	41.0	33.0	41.0
32-33	37.89627079164258	40.0	37.0	41.0	33.0	41.0
34-35	37.87716887089731	40.0	37.0	41.0	33.0	41.0
36-37	37.82984924153954	40.0	37.0	41.0	33.0	41.0
38-39	37.85618562181986	40.0	37.0	41.0	33.0	41.0
40-41	37.88943781132542	40.0	37.0	41.0	33.0	41.0
42-43	37.77331338880427	40.0	37.0	41.0	32.5	41.0
44-45	37.66930948978946	40.0	37.0	41.0	32.0	41.0
46-47	37.66517275998059	40.0	37.0	41.0	32.0	41.0
48-49	37.587566799680694	39.5	36.0	41.0	32.0	41.0
50-51	37.488129292464805	39.0	36.0	41.0	32.0	41.0
52-53	37.31844448916756	39.0	36.0	40.5	31.5	41.0
54-55	37.17672514389931	39.0	35.5	40.0	31.0	41.0
56-57	36.955208962257714	38.5	35.0	40.0	31.0	41.0
58-59	36.70132743948389	38.0	35.0	40.0	31.0	41.0
60-61	36.52875033448427	38.0	35.0	40.0	31.0	41.0
62-63	36.18170007209203	37.0	34.5	40.0	31.0	41.0
64-65	36.12823291915065	37.0	34.5	39.5	31.0	41.0
66-67	35.85988346601278	36.0	34.0	39.0	31.0	41.0
68-69	35.58171328275765	36.0	34.0	39.0	31.0	40.0
70-71	35.321873381923545	35.5	34.0	38.5	31.0	40.0
72-73	35.09578715183795	35.0	34.0	37.0	31.0	39.5
74-75	34.824136796001355	35.0	34.0	37.0	31.0	39.0
76-77	32.684300868558836	33.5	31.0	35.0	27.5	36.5
78-79	34.16498052527219	35.0	33.5	36.0	30.5	37.0
80-81	34.214901759208814	35.0	34.0	36.0	31.0	37.0
82-83	34.08236289839212	35.0	34.0	35.0	31.0	37.0
84-85	33.93739142765366	35.0	34.0	35.0	31.0	36.0
86-87	33.82863944910937	35.0	33.0	35.0	31.0	36.0
88-89	33.725925487084794	35.0	33.0	35.0	31.0	36.0
90-91	33.666473988522654	35.0	33.0	35.0	31.0	36.0
92-93	33.61943052088006	35.0	33.0	35.0	31.0	36.0
94-95	33.58390203145383	35.0	33.0	35.0	31.0	35.0
96-97	33.553807952310834	35.0	33.0	35.0	30.5	35.0
98-99	33.59544580816653	35.0	33.0	35.0	31.0	35.0
100-101	32.88015451912473	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2306	1	-2.4599934755009265
2306	2	-0.9786300183421588
2306	3	-2.3837739550325487
2306	4	-1.4859447488357773
2306	5	0.15204367592732382
2306	6	-0.21952155010696828
2306	7	-0.4889072337633351
2306	8	-0.29788054302011346
2306	9	-0.40989167218344136
2306	10-11	-0.40205548079782005
2306	12-13	-0.33156615110336674
2306	14-15	-0.3914903162748615
2306	16-17	-0.33527513952844146
2306	18-19	-0.3969662618738994
2306	20-21	-0.5006712776560533
2306	22-23	-0.5240858806625255
2306	24-25	-0.46048685559092206
2306	26-27	-0.578464312078971
2306	28-29	-0.580172218143872
2306	30-31	-0.5387721428433068
2306	32-33	-0.57628212898355
2306	34-35	-0.5908540898872943
2306	36-37	-0.5876911273793368
2306	38-39	-0.6878065842196079
2306	40-41	-0.5954730578830265
2306	42-43	-0.49211916791796995
2306	44-45	-0.5315777403072346
2306	46-47	-0.4049436247113434
2306	48-49	-0.4702592750261516
2306	50-51	-0.5202466678329074
2306	52-53	-0.3946348762130256
2306	54-55	-0.44886285570457574
2306	56-57	-0.42897093438047307
2306	58-59	-0.45389311053596515
2306	60-61	-0.39158823161555745
2306	62-63	-0.3952393102232321
2306	64-65	-0.45734132230576563
2306	66-67	-0.4616670206839828
2306	68-69	-0.39299490114833446
2306	70-71	-0.39797653854486015
2306	72-73	-0.298764457643955
2306	74-75	-0.27193283537868496
2306	76-77	-0.24288734000255374
2306	78-79	-0.24507080073449572
2306	80-81	-0.2274371906826005
2306	82-83	-0.17864363655834126
2306	84-85	-0.14965013543142192
2306	86-87	-0.1565321655012113
2306	88-89	-0.11961213320291364
2306	90-91	-0.08073137251924578
2306	92-93	-0.17539952016932148
2306	94-95	-0.15560159540019924
2306	96-97	-0.08883480223186524
2306	98-99	-0.12493968264762856
2306	100-101	-0.017262284117741444
2307	1	-1.991475110431292
2307	2	-2.3322252205303577
2307	3	-3.7641440558057084
2307	4	0.3932682984209528
2307	5	0.4059709941768901
2307	6	-0.2508121996151118
2307	7	-0.16619090610744536
2307	8	-0.25489014295458645
2307	9	-0.6100089716434667
2307	10-11	-0.558334009298747
2307	12-13	-0.6376237776644373
2307	14-15	-0.5450661461816892
2307	16-17	-0.6463853667097013
2307	18-19	-0.5387525590521705
2307	20-21	-0.7041741516368347
2307	22-23	-0.6168077735400459
2307	24-25	-0.6523461756606963
2307	26-27	-0.536637514723644
2307	28-29	-0.7603575550905219
2307	30-31	-0.710826348840925
2307	32-33	-0.744332580907674
2307	34-35	-0.751023138019633
2307	36-37	-0.7983573266253856
2307	38-39	-0.7667008356870326
2307	40-41	-0.714603283916837
2307	42-43	-0.6548219992909026
2307	44-45	-0.788908716840993
2307	46-47	-0.6307038008252945
2307	48-49	-0.7552575006481348
2307	50-51	-0.6645551219857708
2307	52-53	-0.7043886908934951
2307	54-55	-0.5916234697683151
2307	56-57	-0.6876277826790016
2307	58-59	-0.6907210507723605
2307	60-61	-0.5230424241409537
2307	62-63	-0.4892582210997034
2307	64-65	-0.49780948908583866
2307	66-67	-0.2797877720896125
2307	68-69	-0.3836041317402774
2307	70-71	-0.25281478735814034
2307	72-73	-0.22100194506681703
2307	74-75	-0.18005250844280596
2307	76-77	-0.0435860688690326
2307	78-79	-0.2179585375545301
2307	80-81	-0.23084458847144873
2307	82-83	-0.22673552146439135
2307	84-85	-0.2354783108303522
2307	86-87	-0.1721907515046226
2307	88-89	-0.1635106709835341
2307	90-91	-0.1862337537221137
2307	92-93	-0.16172143054185995
2307	94-95	-0.15445117834828181
2307	96-97	-0.13753374959275533
2307	98-99	-0.12702123725616588
2307	100-101	0.04974831264147639
2201	1	-3.5225168235952466
2201	2	-1.9667121981474942
2201	3	-2.4783048406806323
2201	4	0.35304026279035483
2201	5	-5.167246253062032
2201	6	-2.3660337806440452
2201	7	-1.180645049561356
2201	8	-0.7367466368520184
2201	9	-0.8746862103381972
2201	10-11	-0.7756103316337075
2201	12-13	-0.8394076127000929
2201	14-15	-0.8101243269147389
2201	16-17	-0.9644746450318493
2201	18-19	-0.7826571221676346
2201	20-21	-1.0725525043077013
2201	22-23	-1.0021005681718904
2201	24-25	-2.6910871073742157
2201	26-27	-2.3236945891902963
2201	28-29	-1.2677625614757346
2201	30-31	-1.1288961670003914
2201	32-33	-1.118045433155764
2201	34-35	-1.0037137094349404
2201	36-37	-1.1029082868577476
2201	38-39	-0.963941277726164
2201	40-41	-1.0633421637469596
2201	42-43	-0.8122646786953709
2201	44-45	-0.8543034971299264
2201	46-47	-0.801922195756859
2201	48-49	-0.8602880021929877
2201	50-51	-0.9447018874941335
2201	52-53	-0.7957408190062196
2201	54-55	-0.6865518473683494
2201	56-57	-0.6679510138369835
2201	58-59	-0.7333291007436884
2201	60-61	-0.6756173504431402
2201	62-63	-0.7639988372327053
2201	64-65	-0.8532847697377406
2201	66-67	-0.7064148500115763
2201	68-69	-0.7124967541087202
2201	70-71	-0.4141589672161743
2201	72-73	-0.4066898842830966
2201	74-75	-0.33555514432935496
2201	76-77	-0.2866649469227127
2201	78-79	-0.3110060577321647
2201	80-81	-0.3167045339068366
2201	82-83	-0.2918400865512112
2201	84-85	-0.42381754405418803
2201	86-87	-0.3034851525549058
2201	88-89	-0.2533119307272287
2201	90-91	-0.27766484280460446
2201	92-93	-0.3152258967088315
2201	94-95	-0.3389402827125636
2201	96-97	-0.29074360675758015
2201	98-99	-0.2942095116478427
2201	100-101	-0.0908428977982112
2202	1	3.856713292374657
2202	2	2.4004860866740927
2202	3	4.7254579018304845
2202	4	4.268703619137714
2202	5	3.150410373995392
2202	6	1.6585047343001804
2202	7	0.8804694255537271
2202	8	0.6413950336308716
2202	9	0.7591855920108657
2202	10-11	0.5969536421652748
2202	12-13	0.6080716976559799
2202	14-15	0.589553948561182
2202	16-17	0.6478728218913474
2202	18-19	0.540151503660276
2202	20-21	0.7709788018773764
2202	22-23	0.7138543069492798
2202	24-25	0.7325053481821442
2202	26-27	0.6780119808188445
2202	28-29	0.7568966781751314
2202	30-31	0.7136083964823854
2202	32-33	0.7368497539242256
2202	34-35	0.7104442626133718
2202	36-37	-0.006549680430246951
2202	38-39	0.18456224965712664
2202	40-41	0.5990680134433788
2202	42-43	0.551062686901318
2202	44-45	0.6558922683300068
2202	46-47	0.6008863231008021
2202	48-49	0.5862831001037279
2202	50-51	0.5552561130731348
2202	52-53	0.5905739171995847
2202	54-55	0.5508404865801353
2202	56-57	0.482740386082142
2202	58-59	0.5465256350000303
2202	60-61	0.44218872346610993
2202	62-63	0.5341784700331544
2202	64-65	0.5309056884358938
2202	66-67	0.41615409616874643
2202	68-69	0.330819587032245
2202	70-71	0.3273046775868522
2202	72-73	0.26258385969065046
2202	74-75	0.192878385343505
2202	76-77	-0.46447868337772746
2202	78-79	0.10764174311868402
2202	80-81	0.18896115622553822
2202	82-83	0.21580139372481
2202	84-85	0.19725027473582202
2202	86-87	0.18703232630830513
2202	88-89	0.15730685083362772
2202	90-91	0.19686147906170248
2202	92-93	0.17724744122385516
2202	94-95	0.18198409161141882
2202	96-97	0.1790052957146031
2202	98-99	0.23735385797373
2202	100-101	-0.2424226096696671
2304	1	3.4085626466819505
2304	2	2.1250076971101635
2304	3	4.837600597467453
2304	4	3.3633806976780427
2304	5	2.771951482336277
2304	6	1.465127821449535
2304	7	0.7128047500839898
2304	8	0.44808574523099054
2304	9	0.5550761653751053
2304	10-11	0.4628983237637172
2304	12-13	0.4427135176063075
2304	14-15	0.4779460022862665
2304	16-17	0.5470138518416476
2304	18-19	0.48740992046523957
2304	20-21	0.7087121335645961
2304	22-23	0.6157058631862569
2304	24-25	0.5790821402688167
2304	26-27	0.5936308156806049
2304	28-29	0.7137353947950658
2304	30-31	0.5821684326547114
2304	32-33	0.6612657833816016
2304	34-35	0.6964164646390856
2304	36-37	0.6103880283506911
2304	38-39	0.6013717998971302
2304	40-41	0.6209935444596582
2304	42-43	0.5537065623550745
2304	44-45	0.6168697243848698
2304	46-47	0.48978983857978164
2304	48-49	0.5801432304839125
2304	50-51	0.5922794758093204
2304	52-53	0.663629632415855
2304	54-55	0.572757683237711
2304	56-57	0.5357004183532865
2304	58-59	0.5188127466491324
2304	60-61	0.49112267101588714
2304	62-63	0.5370023838183897
2304	64-65	0.48785390051008903
2304	66-67	0.44448495004063204
2304	68-69	0.3744826743949119
2304	70-71	0.2670798891912938
2304	72-73	0.23193572949375607
2304	74-75	0.18587231555444106
2304	76-77	-0.16589831207291894
2304	78-79	0.15909082426964716
2304	80-81	0.198632383458353
2304	82-83	0.22875195349124056
2304	84-85	0.18277094646821723
2304	86-87	0.21100318725018496
2304	88-89	0.15622420716716334
2304	90-91	0.19159054435525746
2304	92-93	0.1529572605106324
2304	94-95	0.13777166114607553
2304	96-97	0.18657298752331286
2304	98-99	0.21645122967120045
2304	100-101	-0.049570302132671884
2203	1	2.8116776475047516
2203	2	1.7805674248605747
2203	3	3.6671145072773292
2203	4	1.2341214264169658
2203	5	1.4353313303419313
2203	6	0.820369906496957
2203	7	0.4685259341403025
2203	8	0.33109206081955733
2203	9	0.5022931116907543
2203	10-11	0.46041739030424367
2203	12-13	0.4790179352302957
2203	14-15	0.4790288310366364
2203	16-17	0.561707258979105
2203	18-19	0.5082109516252018
2203	20-21	0.6591091793177668
2203	22-23	0.5943165624886149
2203	24-25	0.6371977492207606
2203	26-27	0.5643421213590116
2203	28-29	0.65582809041193
2203	30-31	0.5690887078659443
2203	32-33	0.5855331478182748
2203	34-35	0.6524709286967791
2203	36-37	0.6356349020613195
2203	38-39	0.6237602700071321
2203	40-41	0.6103223946974126
2203	42-43	0.5285304459763438
2203	44-45	0.5820749993253358
2203	46-47	0.48181781097414955
2203	48-49	0.5274044249592507
2203	50-51	0.49986993277663316
2203	52-53	0.48404831790897873
2203	54-55	0.4729468260417846
2203	56-57	0.47900921674583685
2203	58-59	0.4765549615818827
2203	60-61	0.3977994494449817
2203	62-63	0.5063032944938328
2203	64-65	0.40756858395607054
2203	66-67	0.3397958002305188
2203	68-69	0.27546877646199874
2203	70-71	0.2944279619414516
2203	72-73	0.24866643210828698
2203	74-75	0.18849162505124895
2203	76-77	-0.2685576633734783
2203	78-79	0.1163592362625181
2203	80-81	0.19001928303614335
2203	82-83	0.1710236138712915
2203	84-85	0.17583296078124278
2203	86-87	0.1777914805961487
2203	88-89	0.06884829994043429
2203	90-91	0.07289703242380341
2203	92-93	0.1427272231899508
2203	94-95	0.09499822037564343
2203	96-97	0.13032243549934464
2203	98-99	0.17177787043838322
2203	100-101	-0.08163848925375561
2305	1	2.707387601479631
2305	2	1.4985636024219673
2305	3	3.1861414953143914
2305	4	1.0001800141843447
2305	5	1.610320539485727
2305	6	0.88088930931373
2305	7	0.5089016503892267
2305	8	0.3928322166695182
2305	9	0.5040360508290647
2305	10-11	0.4052949957050842
2305	12-13	0.3937014399331389
2305	14-15	0.3904444847829609
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2208	84-85	-0.02324841509349085
2208	86-87	-0.0015455838994355986
2208	88-89	-0.021823360411211468
2208	90-91	-0.00924150870687157
2208	92-93	-0.04474685834836123
2208	94-95	-0.020815921046015262
2208	96-97	-0.014104612582322318
2208	98-99	-0.017779925303187838
2208	100-101	0.08697566322899775
2215	1	-0.895594572303807
2215	2	-0.21122941198510503
2215	3	0.022529756863832517
2215	4	-0.2805320000290692
2215	5	0.768576258727478
2215	6	0.24738411942126248
2215	7	0.044277533924649504
2215	8	-0.09383767346245264
2215	9	-0.13711424373570935
2215	10-11	-0.1930566793145445
2215	12-13	-0.22747829128396546
2215	14-15	-0.32500452141862013
2215	16-17	-0.22365951713631915
2215	18-19	-0.17533999330840544
2215	20-21	-0.26857635182496153
2215	22-23	-0.2423089000498635
2215	24-25	-0.22072110988495552
2215	26-27	-0.20964092359648134
2215	28-29	-0.22868779332672062
2215	30-31	-0.23497142785791425
2215	32-33	-0.3284432052813955
2215	34-35	-0.3587682236437715
2215	36-37	-0.3085195733598809
2215	38-39	-0.309923994849747
2215	40-41	-0.25650747640133176
2215	42-43	-0.31224989239573375
2215	44-45	-0.22329787478679464
2215	46-47	-0.3173734462216231
2215	48-49	-0.24893085497954814
2215	50-51	-0.2365498258309202
2215	52-53	-0.32055361171993013
2215	54-55	-0.2783510086323773
2215	56-57	-0.2876929301135718
2215	58-59	-0.21362341161999865
2215	60-61	-0.23154491578021208
2215	62-63	-0.2583636496298851
2215	64-65	-0.21118852567879287
2215	66-67	-0.15940837632238214
2215	68-69	-0.13362332049746328
2215	70-71	-0.0962932284402811
2215	72-73	-0.06934672950079346
2215	74-75	-0.03215838900920431
2215	76-77	0.08234749246442163
2215	78-79	-0.05879392962483365
2215	80-81	-0.04418755335181146
2215	82-83	-0.023194533990462674
2215	84-85	0.030365881897530755
2215	86-87	-0.05251849433151534
2215	88-89	0.005850317043396558
2215	90-91	0.004213175073083164
2215	92-93	0.04089022631501393
2215	94-95	1.6499941956737985E-4
2215	96-97	-0.016796053932189636
2215	98-99	-0.016040529063737097
2215	100-101	0.09054378128710283
2214	1	-5.056508801930082
2214	2	-2.584053803505018
2214	3	-4.5244077751162735
2214	4	-3.5304289677980414
2214	5	-0.927691784749392
2214	6	-0.6245913601968951
2214	7	-0.4648462820034993
2214	8	-0.3942416593053295
2214	9	-0.47885262560134834
2214	10-11	-0.46130255839205603
2214	12-13	-0.3694913285172561
2214	14-15	-0.4581410566464754
2214	16-17	-0.4717205076305575
2214	18-19	-0.4932606380693656
2214	20-21	-0.5639267259768133
2214	22-23	-0.6748600412964194
2214	24-25	-0.518505039466298
2214	26-27	-0.5762242724711584
2214	28-29	-0.5593760605627622
2214	30-31	-0.4999848147496664
2214	32-33	-0.5205360934565064
2214	34-35	-0.5942559778230461
2214	36-37	-0.5558755725517344
2214	38-39	-0.5193779194564101
2214	40-41	-0.6077458730245127
2214	42-43	-0.5214343006485933
2214	44-45	-0.5754922483384775
2214	46-47	-0.5518008566138946
2214	48-49	-0.583896361459523
2214	50-51	-0.5687860111268535
2214	52-53	-0.5351164231891445
2214	54-55	-0.5250814603506555
2214	56-57	-0.4621173359826969
2214	58-59	-0.5204353136929498
2214	60-61	-0.4979038498145627
2214	62-63	-0.5081996767540744
2214	64-65	-0.40710027123225245
2214	66-67	-0.33294002056833705
2214	68-69	-0.26127792847490383
2214	70-71	-0.30424942057072
2214	72-73	-0.275882001376921
2214	74-75	-0.22044614592331158
2214	76-77	0.18388693771508713
2214	78-79	-0.19494627683294397
2214	80-81	-0.26163901095690534
2214	82-83	-0.25252691835570573
2214	84-85	-0.25777611549764856
2214	86-87	-0.2373200570012628
2214	88-89	-0.21318471870542766
2214	90-91	-0.17316793116954443
2214	92-93	-0.17848915862759895
2214	94-95	-0.17559846518453526
2214	96-97	-0.1676429278332492
2214	98-99	-0.15963267301290784
2214	100-101	-0.029993200239417206
2213	1	-3.0298330912907474
2213	2	-1.4540526934190616
2213	3	-2.751410251388311
2213	4	-1.0371941821296957
2213	5	0.45200816198344995
2213	6	-0.11210182438546923
2213	7	-0.2138369588871214
2213	8	-0.4498483851523716
2213	9	-0.5313688367284755
2213	10-11	-0.4064324896389664
2213	12-13	-0.6047596086618086
2213	14-15	-0.6724339561429602
2213	16-17	-0.5704458052976307
2213	18-19	-0.5328943202649086
2213	20-21	-0.7529044488140357
2213	22-23	-0.6280825962364602
2213	24-25	-0.5995326870763407
2213	26-27	-0.4691121611067146
2213	28-29	-0.6432238469691995
2213	30-31	-0.5799545361800682
2213	32-33	-0.5742991639069572
2213	34-35	-0.6822154093376511
2213	36-37	-0.6579730543703377
2213	38-39	-0.6558563873006875
2213	40-41	-0.5777715884451311
2213	42-43	-0.5232923578357926
2213	44-45	-0.5591225008028147
2213	46-47	-0.5318264551342367
2213	48-49	-0.5624968223933209
2213	50-51	-0.5594016157940445
2213	52-53	-0.5230427987010984
2213	54-55	-0.38849610987293914
2213	56-57	-0.4530664067123098
2213	58-59	-0.528150409572909
2213	60-61	-0.4223324778781645
2213	62-63	-0.4777286004613046
2213	64-65	-0.39733578947046055
2213	66-67	-0.39028990238728056
2213	68-69	-0.25731473131805416
2213	70-71	-0.2807722525130245
2213	72-73	-0.2042183855790256
2213	74-75	-0.16241485226660046
2213	76-77	0.06732302649051292
2213	78-79	-0.10402607130203023
2213	80-81	-0.1041734341778735
2213	82-83	-0.11204568096589185
2213	84-85	-0.09517291691860663
2213	86-87	-0.1509408304208577
2213	88-89	-0.1455263621652776
2213	90-91	-0.1695611445305829
2213	92-93	-0.13700518037566667
2213	94-95	-0.0904856707412165
2213	96-97	-0.11516900380323136
2213	98-99	-0.15960217440540703
2213	100-101	0.05653372988854244
2212	1	-3.7003958055418096
2212	2	-5.8752610211177405
2212	3	-0.27332110080118355
2212	4	2.4890555856175034
2212	5	-4.196216134138776
2212	6	-1.9027784015702522
2212	7	-1.301250283111429
2212	8	-1.0670646450007055
2212	9	-1.3747217629180142
2212	10-11	-1.2174765921164479
2212	12-13	-1.0934159880696583
2212	14-15	-1.088532294580368
2212	16-17	-1.1965485349017513
2212	18-19	-1.0002717142193305
2212	20-21	-1.5420691337962467
2212	22-23	-1.1973438965337309
2212	24-25	-1.024901046017881
2212	26-27	-1.0865899547493356
2212	28-29	-1.3874500157099519
2212	30-31	-1.4346935606335336
2212	32-33	-1.2090209073413192
2212	34-35	-1.2160698132047258
2212	36-37	-1.4673559654686628
2212	38-39	-1.2768483154364674
2212	40-41	-1.3260589038889705
2212	42-43	-1.1934832943751417
2212	44-45	-1.2711215112960446
2212	46-47	-1.1495300111315103
2212	48-49	-1.138010215099719
2212	50-51	-1.0549354046962378
2212	52-53	-1.2829460591606434
2212	54-55	-1.0723749814901495
2212	56-57	-1.1011079459838413
2212	58-59	-1.0683237849379807
2212	60-61	-0.8171969091341964
2212	62-63	-1.238174507901192
2212	64-65	-0.9758641408724174
2212	66-67	-0.8711913102527831
2212	68-69	-0.565691569631646
2212	70-71	-0.37646042277122405
2212	72-73	-0.25808786642713244
2212	74-75	-0.19573314582423507
2212	76-77	0.08351161028330978
2212	78-79	-0.24312812441971943
2212	80-81	-0.3079018456607301
2212	82-83	-0.28091078300601424
2212	84-85	-0.3089449851238868
2212	86-87	-0.3509047422914833
2212	88-89	-0.3778763468296944
2212	90-91	-0.43915750893838634
2212	92-93	-0.3953833033255947
2212	94-95	-0.4771148505376033
2212	96-97	-0.3501922777645703
2212	98-99	-0.32832114851594696
2212	100-101	-0.1930934950255292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	265.0
15	5232.0
16	5501.0
17	4740.0
18	3406.0
19	2538.0
20	2904.0
21	1976.0
22	2621.0
23	3585.0
24	6970.0
25	15209.0
26	32012.0
27	63085.0
28	107293.0
29	164491.0
30	228310.0
31	298997.0
32	380147.0
33	485575.0
34	634195.0
35	871977.0
36	1308181.0
37	1886864.0
38	1335379.0
39	179180.0
40	3392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.375590068489954	27.018760832832854	13.44313715727795	47.16251194139924
2	16.532133693371367	26.099936195896273	24.546046124666198	32.821883986066155
3	11.509005029888112	17.8902110102743	30.48095511473222	40.119828845105374
4	16.107687715041603	19.19885682275385	14.118871652972874	50.574583809231676
5	19.651364298486264	23.13077647867942	18.290002093425112	38.927857129409205
6	16.00017616763773	31.428106947729738	21.26574201946253	31.30597486517
7	9.148344920095157	37.759940702675294	27.22083485100898	25.87087952622057
8	8.116341411363138	40.44317088737449	23.69771442792891	27.74277327333346
9	8.207570017547557	45.491249765963886	24.843924922088924	21.457255294399634
10-11	8.494468955995336	41.96653831288775	27.10817817905799	22.43081455205893
12-13	8.092189767867815	45.06616315913526	24.210877292346883	22.630769780650045
14-15	8.540679206770578	41.77734249379842	23.66657872230151	26.01539957712949
16-17	8.61168869632274	43.55440850609465	23.84846751083966	23.98543528674295
18-19	11.676861760926025	37.062630831518554	24.689426902678267	26.571080504877152
20-21	14.388372587859477	37.232840728700914	24.647363682336675	23.73142300110294
22-23	8.012642619591642	40.26842344012379	24.576648823223472	27.142285117061093
24-25	8.021574515951835	40.02626843023171	24.575433849741998	27.376723204074455
26-27	8.171880607249806	40.184499120654635	27.460277344592164	24.18334292750339
28-29	8.181196373885768	39.774198889594416	24.14904984634603	27.89555489017378
30-31	8.02438104496849	36.913161495301914	27.596097723147135	27.46635973658246
32-33	8.151814029757254	40.153801405892544	25.598341135398105	26.096043428952093
34-35	8.337337746908872	39.77814146324087	25.251075940321194	26.63344484952906
36-37	8.316985117477506	39.564966173086304	25.481875874347242	26.636172835088946
38-39	8.255120664250676	39.80920960062369	25.63016465052823	26.305505084597414
40-41	8.293126461836128	39.717532696146264	25.48259088385725	26.506749958160363
42-43	8.221430383421088	39.43471937389527	25.66083495043538	26.683015292248257
44-45	8.309099435643684	39.45578781177687	25.69392346643212	26.541189286147322
46-47	8.383158862745736	39.12334264416513	25.61220847778356	26.881290015305577
48-49	8.264071191646986	39.51753978394703	25.67698532543462	26.54140369897136
50-51	8.292992686413598	39.58218342126445	25.672762886324335	26.452061005997614
52-53	8.25912267491557	39.48075197363799	25.70886257241715	26.551262779029283
54-55	8.146977799384217	39.537763764855946	25.929953975159197	26.38530446060064
56-57	8.189449466913533	39.409581735274166	25.84485707958157	26.556111718230742
58-59	8.19650492755368	39.43827783052956	25.90807315953508	26.457144082381685
60-61	8.129592513534591	39.63189018102913	25.893107971452867	26.345409333983415
62-63	8.113188751543632	39.67565477249778	25.867779415598065	26.343377060360524
64-65	8.178555073316646	39.50562139393165	25.753218232769804	26.562605299981907
66-67	8.122055271825625	39.5480117557887	26.015440168348054	26.314492804037627
68-69	8.063595966460234	39.730422966448714	26.071893014889024	26.134088052202024
70-71	8.051331330753955	39.598405097069666	26.048987343913055	26.30127622826332
72-73	7.985513474962687	39.57940898390527	26.230373618524442	26.204703922607596
74-75	7.974665799355423	39.83851852996075	26.101370567295785	26.085445103388043
76-77	8.009839486207987	39.54600802658626	26.032095585156846	26.412056902048914
78-79	7.927908293254919	39.77358800620829	26.109816362298183	26.1886873382386
80-81	8.03563465419931	39.895885269799315	26.131983543078345	25.93649653292303
82-83	7.907411228879782	39.71618617690677	26.03893458617434	26.33746800803911
84-85	7.83200333576102	39.71285095859386	26.221947696603365	26.233198009041754
86-87	8.04544457300396	40.28798705248677	26.010279153847783	25.65628922066148
88-89	8.331741728082939	40.26789724552748	25.78756383467404	25.612797191715543
90-91	8.526847925527717	40.01934310822039	25.83836654751193	25.615442418739963
92-93	9.531399175840125	39.82412999943555	25.519470944000773	25.124999880723554
94-95	9.920786205607355	40.44907817327671	24.88338034680581	24.74675527431012
96-97	10.564462143219986	40.65161625033421	24.518058772483357	24.265862833962444
98-99	11.032404774590916	40.363108640401386	24.05583159650477	24.54865498850293
100-101	11.188277773286003	40.686622583903606	23.350290498137447	24.77480914467294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2413.0
1	2408.5
2	2309.0
3	2070.5
4	1933.0
5	1952.0
6	1992.5
7	2012.0
8	2035.5
9	2148.5
10	2475.0
11	2889.5
12	3267.5
13	4501.5
14	6173.0
15	7647.0
16	9579.5
17	12309.0
18	16110.0
19	20924.5
20	27324.0
21	36336.5
22	49042.5
23	67728.5
24	94560.5
25	130229.0
26	175510.5
27	230276.5
28	291763.0
29	352421.5
30	407480.5
31	454537.5
32	489209.0
33	509782.5
34	514857.0
35	506842.5
36	489175.0
37	463625.5
38	433033.0
39	399938.5
40	365141.5
41	328754.5
42	294302.0
43	261356.0
44	229617.0
45	201722.5
46	176596.5
47	154335.0
48	134913.5
49	119666.5
50	110297.0
51	99008.0
52	81420.0
53	65695.0
54	55020.5
55	47224.5
56	41434.0
57	36810.5
58	33143.5
59	30051.0
60	27460.5
61	25268.5
62	23346.5
63	21317.0
64	19498.5
65	18331.0
66	17174.0
67	16132.5
68	15284.0
69	14396.5
70	13300.5
71	12443.0
72	11920.0
73	11401.0
74	10953.5
75	10355.5
76	9833.5
77	9568.0
78	9345.5
79	8901.0
80	8482.5
81	8250.0
82	8054.5
83	7695.5
84	7354.5
85	7306.0
86	7240.5
87	7184.5
88	6131.5
89	5074.5
90	4615.0
91	4162.5
92	3770.0
93	3224.5
94	2982.5
95	2879.0
96	2823.0
97	2787.0
98	2776.5
99	2772.5
100	2772.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.472477407942079E-4
3	0.010432452546346295
4	0.010684387308292766
5	4.2391990507186503E-4
6	3.742937077734316E-5
7	8.737809663293497E-5
8	1.6233705418186494E-4
9	2.4980499597501703E-5
10-11	0.0
12-13	0.0
14-15	5.6254359712877744E-5
16-17	6.2522375195022925E-6
18-19	3.064491536437242E-4
20-21	5.6307412445414656E-5
22-23	9.576891673963047E-4
24-25	5.0113830433715784E-5
26-27	5.645471184197799E-5
28-29	5.341178791875628E-4
30-31	6.800100591117633E-4
32-33	4.101959828055941E-4
34-35	8.794342466627052E-4
36-37	0.0
38-39	8.269286122070566E-5
40-41	4.4645935092846644E-5
42-43	0.003031774532172884
44-45	8.724768578721084E-4
46-47	0.003924847029087041
48-49	0.0031942294792534796
50-51	0.0049192832992597454
52-53	6.492336608176124E-6
54-55	0.0
56-57	0.0
58-59	3.278543193757444E-5
60-61	3.948389547323061E-5
62-63	1.321176222051673E-4
64-65	1.989920521911048E-5
66-67	1.1995144365560821E-4
68-69	0.0
70-71	2.9648454901095114E-4
72-73	6.781953547144818E-6
74-75	0.0
76-77	0.0015932628844445055
78-79	1.1140939429079773E-4
80-81	0.0
82-83	3.7066164600715076E-4
84-85	0.0
86-87	0.0
88-89	0.0012032609684894393
90-91	4.388844289290671E-5
92-93	6.606075739979574E-5
94-95	0.0
96-97	7.412512706899907E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	13642.0
5-9	15768.0
10-14	5819.0
15-19	6143.0
20-24	12815.0
25-29	34653.0
30-34	47593.0
35-39	52814.0
40-44	56667.0
45-49	57392.0
50-54	60013.0
55-59	65601.0
60-64	75463.0
65-69	98012.0
70-74	131937.0
75-79	175986.0
80-84	234735.0
85-89	47966.0
90-94	65842.0
95-99	139667.0
100-102	6635497.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.01377844989534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.65262837328197	77.00083614503502
2	5.594816857847976	9.40083407925959
3	1.26206350865152	3.180921720166391
4	0.5162199874608476	1.7347836663177358
5	0.2942814477004568	1.236184817451032
6	0.15117985637597975	0.7620714575795137
7	0.11477007815403285	0.6749587543097063
8	0.0930316132965164	0.6252749878663892
9	0.07115313208961982	0.5380059127854111
>10	0.23609069001259703	3.296587385545539
>50	0.00907377874542751	0.5158466123755626
>100	0.004336394094607579	0.7004408724593694
>500	2.458101883214222E-4	0.13777167039915877
>1k	9.351180051349171E-5	0.13072790924351452
>5k	1.4960299606704127E-5	0.06475400920607143
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.734118327986283E-5	0.0	0.0
2	3.734118327986283E-5	0.0	1.2447061093287611E-5
3	3.734118327986283E-5	0.0	1.2447061093287611E-5
4	3.734118327986283E-5	0.0	1.2447061093287611E-5
5	7.468236655972566E-5	0.0	1.2447061093287611E-5
6	7.468236655972566E-5	0.0	1.2447061093287611E-5
7	7.468236655972566E-5	0.0	1.2447061093287611E-5
8	7.468236655972566E-5	0.0	1.2447061093287611E-5
9	7.468236655972566E-5	0.0	1.2447061093287611E-5
10-11	7.468236655972566E-5	0.0	1.2447061093287611E-5
12-13	8.712942765301327E-5	0.0	1.2447061093287611E-5
14-15	8.712942765301327E-5	6.2235305466438054E-6	1.2447061093287611E-5
16-17	1.120235498395885E-4	1.2447061093287611E-5	1.2447061093287611E-5
18-19	1.120235498395885E-4	1.2447061093287611E-5	1.2447061093287611E-5
20-21	1.244706109328761E-4	1.2447061093287611E-5	1.2447061093287611E-5
22-23	1.369176720261637E-4	1.2447061093287611E-5	1.2447061093287611E-5
24-25	1.7425885530602655E-4	2.4894122186575222E-5	1.2447061093287611E-5
26-27	1.9292944694595797E-4	3.734118327986283E-5	1.2447061093287611E-5
28-29	1.9915297749260177E-4	5.601177491979424E-5	1.2447061093287611E-5
30-31	2.3649416077246459E-4	6.223530546643805E-5	1.2447061093287611E-5
32-33	2.676118135056836E-4	6.223530546643805E-5	1.2447061093287611E-5
34-35	2.738353440523274E-4	6.223530546643805E-5	1.2447061093287611E-5
36-37	2.9250593569225884E-4	6.223530546643805E-5	1.2447061093287611E-5
38-39	3.422941800654093E-4	6.223530546643805E-5	1.2447061093287611E-5
40-41	3.671883022519845E-4	6.223530546643805E-5	1.2447061093287611E-5
42-43	3.9830595498520355E-4	6.223530546643805E-5	1.2447061093287611E-5
44-45	4.605412604516416E-4	6.223530546643805E-5	1.2447061093287611E-5
46-47	5.41447157558011E-4	6.223530546643805E-5	1.2447061093287611E-5
48-49	5.725648102912301E-4	6.223530546643805E-5	1.2447061093287611E-5
50-51	6.72141299037531E-4	8.712942765301327E-5	1.2447061093287611E-5
52-53	7.0325895177075E-4	8.712942765301327E-5	1.8670591639931416E-5
54-55	7.094824823173938E-4	8.712942765301327E-5	2.4894122186575222E-5
56-57	7.841648488771195E-4	8.712942765301327E-5	2.4894122186575222E-5
58-59	8.96188398716708E-4	8.712942765301327E-5	2.4894122186575222E-5
60-61	0.001020659009649584	8.712942765301327E-5	4.9788244373150444E-5
62-63	0.0011326825594891725	8.712942765301327E-5	4.9788244373150444E-5
64-65	0.001213588456595542	8.712942765301327E-5	4.9788244373150444E-5
66-67	0.001344282598075062	8.712942765301327E-5	4.9788244373150444E-5
68-69	0.0015434355755676637	9.957648874630089E-5	4.9788244373150444E-5
70-71	0.0021782356913253318	9.957648874630089E-5	4.9788244373150444E-5
72-73	0.003864812469465803	9.957648874630089E-5	4.9788244373150444E-5
74-75	0.0069392365595078425	9.957648874630089E-5	4.9788244373150444E-5
76-77	0.01089117845662666	9.957648874630089E-5	4.9788244373150444E-5
78-79	0.015540155774969583	9.957648874630089E-5	4.9788244373150444E-5
80-81	0.020581215517751064	9.957648874630089E-5	4.9788244373150444E-5
82-83	0.0265246871897959	9.957648874630089E-5	4.9788244373150444E-5
84-85	0.033607064951876556	9.957648874630089E-5	4.9788244373150444E-5
86-87	0.9182943792183869	9.957648874630089E-5	4.9788244373150444E-5
88-89	2.190060399363955	9.957648874630089E-5	4.9788244373150444E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATA	81595	0.0	55.708027	5
TCATAAA	86770	0.0	52.825	7
CACGGGC	875	0.0	52.673	3
ATCATAA	88335	0.0	52.34521	6
TAAATCA	78970	0.0	51.308014	3
AAATCAT	86915	0.0	50.443287	4
CATAAAC	91300	0.0	50.251392	8
CACACGG	925	0.0	49.79191	1
AGAGCAC	10460	0.0	48.229042	94-95
ATAAATC	86840	0.0	46.27217	2
ACACGGG	1010	0.0	45.62286	2
GAGCACA	6000	0.0	41.722885	94-95
CCAACGG	790	0.0	41.339233	2
AATAAAT	98965	0.0	40.561394	1
ACCGGGC	585	0.0	39.766205	2
AACCGGG	665	0.0	39.67287	1
ATAAACA	118370	0.0	38.981358	9
ATACGGC	800	0.0	38.598904	5
TACGGCG	865	0.0	35.18522	6
CCGGGCA	680	0.0	34.217896	3
>>END_MODULE