##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 20150414_trimmed_2212_lane2_CTTGTA_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15922161 Sequences flagged as poor quality 0 Sequence length 1-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.978313245293776 31.0 28.0 33.0 16.0 34.0 2 29.798456627643034 31.0 30.0 34.0 25.0 34.0 3 30.574047038884697 31.0 30.0 34.0 26.0 34.0 4 33.505370848809314 35.0 33.0 37.0 28.0 37.0 5 34.15338931272001 35.0 35.0 37.0 28.0 37.0 6 34.89130442433162 35.0 35.0 37.0 32.0 37.0 7 35.00194754397814 36.0 35.0 37.0 32.0 37.0 8 35.016155548551744 36.0 35.0 37.0 32.0 37.0 9 36.653083043160436 39.0 35.0 39.0 32.0 39.0 10-11 36.67635309013272 39.0 35.0 39.0 32.0 39.0 12-13 36.748619858532734 39.0 36.0 39.0 32.0 39.0 14-15 37.785714699674074 39.5 37.0 41.0 32.5 41.0 16-17 37.799087292920404 39.0 37.0 41.0 33.0 41.0 18-19 37.91697715107892 40.0 37.0 41.0 32.5 41.0 20-21 38.133408772997804 40.0 37.0 41.0 33.0 41.0 22-23 38.17545352708014 40.0 37.0 41.0 33.0 41.0 24-25 38.08461467849867 40.0 37.0 41.0 33.0 41.0 26-27 37.97519969047757 40.0 37.0 41.0 33.0 41.0 28-29 37.849202921919584 40.0 37.0 41.0 33.0 41.0 30-31 37.82867107243044 40.0 37.0 41.0 33.0 41.0 32-33 37.74482594808278 40.0 37.0 41.0 32.5 41.0 34-35 37.69488602281412 40.0 37.0 41.0 32.0 41.0 36-37 37.626336588409416 40.0 37.0 41.0 32.0 41.0 38-39 37.57596125751965 40.0 36.5 41.0 32.0 41.0 40-41 37.50194912102507 40.0 36.0 41.0 32.0 41.0 42-43 37.43160781662016 39.0 36.0 41.0 32.0 41.0 44-45 37.34481827662378 39.0 36.0 41.0 31.5 41.0 46-47 37.23470913800436 39.0 35.0 41.0 31.0 41.0 48-49 37.141610110613755 39.0 35.0 41.0 31.0 41.0 50-51 37.047937575636276 39.0 35.0 41.0 31.0 41.0 52-53 36.911000706771496 39.0 35.0 40.0 31.0 41.0 54-55 36.77442954532795 39.0 35.0 40.0 31.0 41.0 56-57 36.639643345429874 38.0 35.0 40.0 31.0 41.0 58-59 36.47824159206219 38.0 35.0 40.0 31.0 41.0 60-61 36.288635612362704 38.0 34.0 40.0 30.5 41.0 62-63 36.03507357335649 37.0 34.0 40.0 30.0 41.0 64-65 35.740600094367984 37.0 34.0 39.0 30.0 41.0 66-67 35.376966822215756 36.0 34.0 39.0 30.0 40.0 68-69 35.03994918283085 35.5 34.0 38.5 30.0 40.0 70-71 34.71522438413119 35.0 33.5 37.0 30.0 39.0 72-73 34.37605352946082 35.0 33.0 37.0 29.0 39.0 74-75 34.027230551995146 35.0 33.0 36.5 29.0 38.5 76-77 31.245874461165016 32.5 29.5 34.5 25.0 35.5 78-79 33.31444984732386 34.5 32.5 35.0 29.0 37.0 80-81 33.49372981826119 35.0 33.0 35.0 29.0 36.0 82-83 33.40235864204985 35.0 33.0 35.0 29.0 36.0 84-85 33.29429255073265 35.0 33.0 35.0 29.0 36.0 86-87 33.23387574976369 35.0 33.0 35.0 29.0 35.5 88-89 33.15418642035781 35.0 33.0 35.0 29.0 35.0 90-91 33.12505647976329 35.0 33.0 35.0 29.0 35.0 92-93 33.05369817269086 34.5 33.0 35.0 29.0 35.0 94-95 33.01689930489344 34.0 33.0 35.0 29.0 35.0 96-97 33.0503527514208 34.0 33.0 35.0 29.0 35.0 98-99 33.15584668383232 34.0 33.0 35.0 29.0 35.0 100-101 32.405744340423766 33.5 31.5 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 2.3623282754569637 1101 2 1.904218092348632 1101 3 1.3763700907385115 1101 4 1.4957148851410764 1101 5 1.5191915883042881 1101 6 0.7345582943234774 1101 7 0.31290054878679996 1101 8 0.13305105247710713 1101 9 0.09207575853663741 1101 10-11 0.06853244517218116 1101 12-13 0.09136504024377956 1101 14-15 0.043637516451084934 1101 16-17 0.02899167615342435 1101 18-19 0.16731911737979033 1101 20-21 0.4001220286874627 1101 22-23 0.3941677845705911 1101 24-25 0.4141707767874152 1101 26-27 0.4038291160169507 1101 28-29 0.34374994091523803 1101 30-31 0.3611264916429562 1101 32-33 0.420133067984537 1101 34-35 0.4388358824829197 1101 36-37 0.3917434606186916 1101 38-39 0.4148167523127526 1101 40-41 0.4314637926025213 1101 42-43 0.46282223157196967 1101 44-45 0.34821779414479437 1101 46-47 0.377746127675465 1101 48-49 0.3382317969361779 1101 50-51 0.3789862143800562 1101 52-53 0.3873125050283619 1101 54-55 0.3414351822224475 1101 56-57 0.3420721741665247 1101 58-59 0.32149122532877783 1101 60-61 0.3388916028650044 1101 62-63 0.37413111185632886 1101 64-65 0.32713334539005245 1101 66-67 0.2706652401682206 1101 68-69 0.3231706325320829 1101 70-71 0.28137165232115535 1101 72-73 0.2390674624493201 1101 74-75 0.23699887776847817 1101 76-77 0.15526678216762235 1101 78-79 0.18144208215743163 1101 80-81 0.15285249082685937 1101 82-83 0.17010832488151095 1101 84-85 0.13705739914405513 1101 86-87 0.19498867965172195 1101 88-89 0.13822738540989832 1101 90-91 0.0741481319794488 1101 92-93 0.11339289329954028 1101 94-95 0.10311381161206157 1101 96-97 0.06316363503935918 1101 98-99 0.11128152695728488 1101 100-101 0.10010759637925304 1102 1 2.188845740089139 1102 2 1.8753407572416059 1102 3 1.424074987169739 1102 4 0.5329470877664093 1102 5 1.251309749866806 1102 6 0.6110144780720717 1102 7 0.34160438647657543 1102 8 0.17331910185569654 1102 9 0.16401486238071783 1102 10-11 0.10202990417863589 1102 12-13 0.16814179672230267 1102 14-15 0.13936013566409144 1102 16-17 0.18602557244000906 1102 18-19 0.2235854238147823 1102 20-21 0.37262578924199374 1102 22-23 0.3451477042114277 1102 24-25 0.37183784071374504 1102 26-27 0.2570264115286136 1102 28-29 0.2503482577159346 1102 30-31 0.2139443105954726 1102 32-33 0.27388503774509587 1102 34-35 0.2853668283762403 1102 36-37 0.3751871961294526 1102 38-39 0.39695965052526816 1102 40-41 0.4051082872577112 1102 42-43 0.11359079165085717 1102 44-45 0.3408862603636038 1102 46-47 0.4087531272712752 1102 48-49 0.3472492477973006 1102 50-51 0.27483531781975756 1102 52-53 0.2835197167797716 1102 54-55 0.3280357095358326 1102 56-57 0.2881650144123924 1102 58-59 0.18892443343487741 1102 60-61 0.26641782843437056 1102 62-63 0.2252134514473596 1102 64-65 0.27306873732283066 1102 66-67 0.2586462657576547 1102 68-69 0.2593981618259278 1102 70-71 0.1912627660859343 1102 72-73 0.18837435946760195 1102 74-75 0.17736589735963548 1102 76-77 -0.06721152666559504 1102 78-79 0.14450200271364366 1102 80-81 0.174803509968811 1102 82-83 0.15908774888355737 1102 84-85 0.1287642515722638 1102 86-87 0.17143319433598236 1102 88-89 0.1326693800550558 1102 90-91 0.11810072005657446 1102 92-93 0.13935363448360505 1102 94-95 0.1278396754463813 1102 96-97 0.11168126999119465 1102 98-99 0.07191931822894304 1102 100-101 0.09575885542449925 1103 1 2.1995747723590746 1103 2 1.8064721535106756 1103 3 1.27587294877263 1103 4 0.8113849130628736 1103 5 1.260821031410785 1103 6 0.5922641955578385 1103 7 0.31736935439214875 1103 8 0.20741153637791854 1103 9 0.17705686357383144 1103 10-11 0.10862934493875542 1103 12-13 0.13772736751673165 1103 14-15 0.19489462367735655 1103 16-17 0.17435821925862172 1103 18-19 0.16461626597401846 1103 20-21 0.29361569681914546 1103 22-23 0.11873781273788353 1103 24-25 0.2899646449283182 1103 26-27 0.2681296185954807 1103 28-29 0.24203048218885925 1103 30-31 0.3274119579663832 1103 32-33 0.33663324188002974 1103 34-35 0.20243721854886587 1103 36-37 0.2789498076929391 1103 38-39 0.29088315686272637 1103 40-41 0.30901330653461656 1103 42-43 0.32476782744675603 1103 44-45 0.3612911488475774 1103 46-47 0.31987644908478785 1103 48-49 0.14877175272364696 1103 50-51 0.32267990144011804 1103 52-53 0.19203404894575726 1103 54-55 0.22765222307800315 1103 56-57 0.28666413793893497 1103 58-59 0.26859391114657427 1103 60-61 0.2680659857180032 1103 62-63 0.299523320173094 1103 64-65 0.3538365562254029 1103 66-67 0.2770829813538711 1103 68-69 0.20683722570795737 1103 70-71 0.17290888906843094 1103 72-73 0.1875465364623139 1103 74-75 0.14411920399956557 1103 76-77 -0.008518475867454356 1103 78-79 0.18389792803797178 1103 80-81 0.17515455275591307 1103 82-83 0.15176980820849906 1103 84-85 0.15633412282223702 1103 86-87 0.13137675487968892 1103 88-89 0.06890278836309705 1103 90-91 0.09031068904356232 1103 92-93 0.09284713414093204 1103 94-95 0.16227521596334782 1103 96-97 0.02373073128720904 1103 98-99 0.14921093807380004 1103 100-101 0.12035111219032757 1116 1 1.840394183543765 1116 2 1.599059334354454 1116 3 0.9260300208923944 1116 4 0.09513187049991245 1116 5 1.0146966659192174 1116 6 0.4546517527601779 1116 7 0.19295593428321212 1116 8 0.06412426405411509 1116 9 0.09005154808786386 1116 10-11 0.11080876428648168 1116 12-13 0.11584016351034876 1116 14-15 0.08873728284086724 1116 16-17 0.09332270051748992 1116 18-19 0.1733917856651317 1116 20-21 0.2683656116885018 1116 22-23 0.14475366839673143 1116 24-25 0.23008605223586187 1116 26-27 0.2033191471625173 1116 28-29 0.18317973864869685 1116 30-31 0.20787891645941414 1116 32-33 0.1259474304366961 1116 34-35 0.1557348484345198 1116 36-37 0.14503152005401887 1116 38-39 0.10773118534796566 1116 40-41 0.002963007188746758 1116 42-43 0.015209265971542152 1116 44-45 0.10521139008726976 1116 46-47 0.14369865198053589 1116 48-49 0.1805620416662279 1116 50-51 0.2444377570206271 1116 52-53 0.2399210010895132 1116 54-55 0.26807225741449514 1116 56-57 0.21074843877721605 1116 58-59 0.21382492501358996 1116 60-61 0.02754594056141002 1116 62-63 0.16369092637019378 1116 64-65 0.22436472058580392 1116 66-67 0.14705672560990735 1116 68-69 0.09556450502792302 1116 70-71 0.09383826600390677 1116 72-73 0.16774011088459417 1116 74-75 0.15707859523112688 1116 76-77 -0.029110522059561106 1116 78-79 0.0675823431001632 1116 80-81 0.1455972071305638 1116 82-83 0.05645860052172935 1116 84-85 0.12342496340569653 1116 86-87 0.1185612979407793 1116 88-89 0.12712479555478495 1116 90-91 0.12884538996559058 1116 92-93 0.07242972603339837 1116 94-95 -0.009365448837144186 1116 96-97 0.05927292375658055 1116 98-99 0.05244621234730573 1116 100-101 0.059763007403155655 1113 1 0.9050797056561954 1113 2 1.0559253142182463 1113 3 0.6236100247165659 1113 4 1.30687115871973 1113 5 1.2824095678897294 1113 6 0.48843624087088955 1113 7 0.3357295836648717 1113 8 0.17624860309877732 1113 9 0.12582937230307323 1113 10-11 0.10630174710849616 1113 12-13 0.09161743866054906 1113 14-15 0.14116863072401742 1113 16-17 0.08450321278279915 1113 18-19 0.16349718447150963 1113 20-21 0.1916755112676114 1113 22-23 0.1512193642888633 1113 24-25 0.16512122214091107 1113 26-27 0.04832244916931927 1113 28-29 0.0580258633967361 1113 30-31 0.1958420083622059 1113 32-33 0.1715164977561443 1113 34-35 0.17868128037098785 1113 36-37 0.24034748924439242 1113 38-39 0.20899453031567816 1113 40-41 0.188366832181174 1113 42-43 0.16631323639115436 1113 44-45 0.21020845548280676 1113 46-47 0.24744305809387868 1113 48-49 0.22524436150441574 1113 50-51 0.11634004035128243 1113 52-53 0.11098109732266437 1113 54-55 0.22960365579309183 1113 56-57 0.12919389511331048 1113 58-59 0.13000657749888234 1113 60-61 0.1414938492978095 1113 62-63 0.11658737439964284 1113 64-65 0.18548950248626284 1113 66-67 0.22303464810300966 1113 68-69 0.11657812615356988 1113 70-71 0.11404371018313242 1113 72-73 0.13891697956943716 1113 74-75 0.09795398933984956 1113 76-77 -0.025425336619139927 1113 78-79 0.08833115555380289 1113 80-81 0.16828866172433266 1113 82-83 0.1491423241580776 1113 84-85 0.1314678704754897 1113 86-87 0.1016667633475663 1113 88-89 0.07039500982492797 1113 90-91 0.029104188811686527 1113 92-93 0.03510450417251576 1113 94-95 0.050553257002306395 1113 96-97 0.027364853470572825 1113 98-99 0.07073767598718916 1113 100-101 0.09046983658534202 1112 1 1.9216254207969357 1112 2 1.7283081788802122 1112 3 1.2620068709007626 1112 4 1.6541092301291442 1112 5 1.4437370386876012 1112 6 0.5914826440745173 1112 7 0.26172789434465926 1112 8 0.13340174098755142 1112 9 0.1350152299782721 1112 10-11 0.02512055813520675 1112 12-13 -0.029897416677485467 1112 14-15 -0.06963338199497571 1112 16-17 0.02399444779429416 1112 18-19 0.011799954568587623 1112 20-21 0.041720751401634004 1112 22-23 0.16415513069474486 1112 24-25 -0.008371345588145118 1112 26-27 0.09227170713420207 1112 28-29 0.10083246998659234 1112 30-31 0.026856448832283775 1112 32-33 0.008606857385565547 1112 34-35 0.11300626566836058 1112 36-37 0.04998190273778391 1112 38-39 -0.09922509949659997 1112 40-41 -0.005396883733936875 1112 42-43 0.0736173181222668 1112 44-45 0.08773223881025416 1112 46-47 -0.020727140300799363 1112 48-49 -0.07217743931617093 1112 50-51 0.004200339771379902 1112 52-53 0.007015813309614316 1112 54-55 0.09862188919477433 1112 56-57 0.14017615775861714 1112 58-59 0.07941550422324894 1112 60-61 0.13668568338161435 1112 62-63 0.10557558721080795 1112 64-65 0.10917810141836526 1112 66-67 0.09566713482283262 1112 68-69 0.11638561398432756 1112 70-71 0.009587629455772628 1112 72-73 0.14239406609399197 1112 74-75 0.15122517049881878 1112 76-77 0.20325118085113303 1112 78-79 0.1968682185818551 1112 80-81 0.21465476628071656 1112 82-83 0.10750052378086394 1112 84-85 0.10970580427205334 1112 86-87 0.11452757901935229 1112 88-89 0.129602172404212 1112 90-91 0.11025854761142284 1112 92-93 0.04893065719364387 1112 94-95 0.1033708064983685 1112 96-97 0.12080097657264588 1112 98-99 0.06386423365368898 1112 100-101 0.13624744639015063 1115 1 1.0466342517224483 1115 2 1.3328742220108971 1115 3 1.0844563738108342 1115 4 1.3810799927404886 1115 5 1.3280293864108046 1115 6 0.5708258011350722 1115 7 0.1693060830895874 1115 8 0.04382876982384687 1115 9 0.0440730102860698 1115 10-11 0.017854150250357748 1115 12-13 0.05658790406357639 1115 14-15 0.007618240161193057 1115 16-17 0.045931539951389766 1115 18-19 0.022804570193301288 1115 20-21 0.085512565203814 1115 22-23 0.08207527784937696 1115 24-25 0.05719529274453805 1115 26-27 0.0579913067671356 1115 28-29 0.08940254574361717 1115 30-31 0.08208898420409128 1115 32-33 0.14945303206224025 1115 34-35 0.13968471191101628 1115 36-37 0.0871292749274204 1115 38-39 0.10300385304575599 1115 40-41 0.15113294768689656 1115 42-43 0.07883241158085497 1115 44-45 0.10434281980106874 1115 46-47 0.10792522396486248 1115 48-49 0.014566307476378881 1115 50-51 0.0693975355142058 1115 52-53 0.07026729631880357 1115 54-55 0.013557751117893702 1115 56-57 0.06483472722566574 1115 58-59 0.10897757023989385 1115 60-61 0.1257905843771141 1115 62-63 0.14239418603474974 1115 64-65 0.11179043920781595 1115 66-67 0.1117752971092898 1115 68-69 0.1711590879634315 1115 70-71 0.16441786166733863 1115 72-73 0.16476010181342815 1115 74-75 0.08822871795508291 1115 76-77 0.21490570442532686 1115 78-79 0.10342337861215611 1115 80-81 0.09997457496496764 1115 82-83 0.14845937677160492 1115 84-85 0.12347017820603412 1115 86-87 0.1529672815107972 1115 88-89 0.11385724839207256 1115 90-91 0.06872635584872455 1115 92-93 0.12363374213367706 1115 94-95 0.153392860637112 1115 96-97 0.047729852111501714 1115 98-99 0.047021311788846276 1115 100-101 0.025790354184259456 1114 1 0.042346334218756 1114 2 0.5947191191617698 1114 3 0.3553543421327596 1114 4 0.28364038415745085 1114 5 0.8741789372056132 1114 6 0.3224896614867987 1114 7 0.11330423320072924 1114 8 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-0.08943471020961624 1209 1 -2.0725620681920063 1209 2 -3.3195134841035454 1209 3 -0.8436398869426434 1209 4 -0.9401271331276888 1209 5 -1.6321265992670604 1209 6 -0.7695824223621699 1209 7 -0.38480861396240584 1209 8 -0.17260914461 1209 9 -0.09221322302363433 1209 10-11 -0.030944029755978875 1209 12-13 -0.08715339129453525 1209 14-15 -0.10018791205121858 1209 16-17 -0.05251874561113823 1209 18-19 -0.11998891648987353 1209 20-21 -0.24180649403837862 1209 22-23 -0.2561052565645099 1209 24-25 -0.23788402246707818 1209 26-27 -0.24423064609257494 1209 28-29 -0.2818626308512009 1209 30-31 -0.31936143483745383 1209 32-33 -0.26884236983688226 1209 34-35 -0.24390760236929054 1209 36-37 -0.24836875650647272 1209 38-39 -0.1900683917818924 1209 40-41 -0.26577910360472856 1209 42-43 -0.23797441700499888 1209 44-45 -0.2739151690387942 1209 46-47 -0.24222919189643477 1209 48-49 -0.23221136757874916 1209 50-51 -0.2519233146543911 1209 52-53 -0.19731182133054403 1209 54-55 -0.2100606126204312 1209 56-57 -0.2594056571222225 1209 58-59 -0.17359539346201558 1209 60-61 -0.2414431086448232 1209 62-63 -0.2377974729059602 1209 64-65 -0.27090438479694967 1209 66-67 -0.16658065640415032 1209 68-69 -0.11178566816417401 1209 70-71 -0.1540101681994983 1209 72-73 -0.1669185560794304 1209 74-75 -0.15806992285924082 1209 76-77 0.11368809166933147 1209 78-79 -0.0691401936924052 1209 80-81 -0.14854694067197727 1209 82-83 -0.13930094783766833 1209 84-85 -0.10293518696625625 1209 86-87 -0.11684069073868386 1209 88-89 -0.10145207939594769 1209 90-91 -0.10341416517923818 1209 92-93 -0.1132089555595357 1209 94-95 -0.09684202054974378 1209 96-97 -0.09721486062656481 1209 98-99 -0.0888318210374095 1209 100-101 -0.06013001632678794 1212 1 -1.338141062156481 1212 2 0.011949738548324262 1212 3 -1.3811875230667745 1212 4 -0.8941730803181187 1212 5 -0.7606394800058354 1212 6 -0.07117097642272796 1212 7 0.06386986284595508 1212 8 0.22383432393157676 1212 9 -0.022751155746341567 1212 10-11 0.17836966486083128 1212 12-13 0.16030619593449558 1212 14-15 0.3474093778175984 1212 16-17 0.11809461221797335 1212 18-19 0.28523022145058974 1212 20-21 -0.08527887500390108 1212 22-23 0.12766725809762391 1212 24-25 0.03621777379505886 1212 26-27 0.23088257967944514 1212 28-29 0.21813927865391491 1212 30-31 0.254141466922313 1212 32-33 0.04154753088083396 1212 34-35 0.24149358234051732 1212 36-37 0.023090520813582316 1212 38-39 0.25002227636254304 1212 40-41 0.23936476362589332 1212 42-43 0.3839668745450595 1212 44-45 0.2078819865681183 1212 46-47 0.06521733431463872 1212 48-49 0.17469606042229202 1212 50-51 0.3835451003729844 1212 52-53 0.20186882389096894 1212 54-55 0.38262483796831503 1212 56-57 0.21118258976553506 1212 58-59 0.2408126539015356 1212 60-61 0.31312075001008566 1212 62-63 0.2836666860462458 1212 64-65 0.20303420409771888 1212 66-67 0.22181852616427022 1212 68-69 0.14021679181077218 1212 70-71 0.3048781356128174 1212 72-73 0.24527375262210427 1212 74-75 0.25935368917744483 1212 76-77 0.3183799440811761 1212 78-79 0.2610084110870048 1212 80-81 0.1792699216996425 1212 82-83 0.08692314973749404 1212 84-85 0.08118877679846292 1212 86-87 0.06073725347540204 1212 88-89 0.19056802996912126 1212 90-91 0.19862636668653266 1212 92-93 0.21858167928775885 1212 94-95 0.2722627395162931 1212 96-97 0.1798010676508568 1212 98-99 0.22762457077298137 1212 100-101 0.3486309618253074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 84.0 15 3100.0 16 2658.0 17 2616.0 18 1729.0 19 1661.0 20 1895.0 21 2005.0 22 2939.0 23 4896.0 24 10609.0 25 26680.0 26 64766.0 27 138284.0 28 247917.0 29 385911.0 30 533948.0 31 692812.0 32 872557.0 33 1104811.0 34 1442180.0 35 1939437.0 36 2467525.0 37 3142115.0 38 2592397.0 39 229857.0 40 6772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.715062861127958 25.532872076849365 15.23676340165132 44.51530166037136 2 16.750148060134247 29.019277438902378 24.071106810049983 30.159467690913388 3 12.199481259337922 18.368627445069087 30.255910887549497 39.17598040804349 4 16.771754754246892 20.945520800205077 14.481471177530347 47.80125326801768 5 19.472026140636867 24.90053498730517 16.824679688729173 38.80275918332878 6 15.862544194926492 31.372622460637768 19.7242538237331 33.04057952070264 7 12.634200476404345 32.66215992389097 23.73206451416217 30.97157508554252 8 11.472788327194309 35.56917212715833 20.54374620692222 32.41429333872514 9 11.487048725466307 40.33444895970068 21.907133144164185 26.271369170668823 10-11 11.900048717189998 37.09366660491189 23.186511172105526 27.819773505792583 12-13 11.15468291292943 40.0241874409651 20.286895237690178 28.534234408415298 14-15 11.558205640176656 37.051697261134834 20.136944674128866 31.253152424559644 16-17 11.389276491656345 39.01641195680123 20.04056081842315 29.553750733119276 18-19 14.290271310148167 32.24496737128223 20.954080874080738 32.51068044448886 20-21 16.908143049758664 33.08343256800968 21.097069074107004 28.911355308124655 22-23 10.360649618593856 36.365755329642155 20.516434818952547 32.75716023281145 24-25 10.321415752125414 35.960446524490905 20.336558751114197 33.38157897226948 26-27 10.36385385158782 36.543261451984385 23.729470081384456 29.363414615043336 28-29 10.44091253112549 36.00467035368476 19.97144081126167 33.58297630392808 30-31 10.327062230856383 32.98374153881154 23.61958299504537 33.0696132352867 32-33 10.360074275693295 36.541216981258295 21.662507665548112 31.436201077500296 34-35 10.431128860408446 36.07725341306498 21.19997336330917 32.29164436321741 36-37 10.36043868545177 35.84729147775164 21.50301273534898 32.28925710144761 38-39 10.379693667751827 36.28490342314412 21.745817472389174 31.589585436714877 40-41 10.41023305767648 36.0367115411128 21.360151736066143 32.19290366514457 42-43 10.300041585595384 35.78774405268291 21.690049942037376 32.22216441968433 44-45 10.318778899576921 36.29261466051729 21.81846240137843 31.57014403852736 46-47 10.391553715994748 35.98574463987931 21.418845297796306 32.20385634632964 48-49 10.269856608048723 35.839628299781154 21.748296662518037 32.14221842965209 50-51 10.26049627332881 36.27377738450134 21.96366951428252 31.502056827887337 52-53 10.291341511800441 35.994604381591216 21.584727378314902 32.12932672829344 54-55 10.198107755171701 35.778490423560235 21.910763479520973 32.112638341747086 56-57 10.21549366944297 36.28254287385722 21.961352748796497 31.540610707903316 58-59 10.254384141076509 36.02930309306748 21.60327727954147 32.11303548631454 60-61 10.154956733550646 35.8301086474045 21.911930723700245 32.103003895344614 62-63 10.132865791304319 36.22894768011184 22.097122848225116 31.541063680358732 64-65 10.182205069322828 35.997894566850015 21.722786422167488 32.097113941659664 66-67 10.062678219941246 35.80474970448138 21.966935572902223 32.16563650267515 68-69 10.058888900991052 36.236124014760826 22.12180809296013 31.583178991287998 70-71 10.085898238946827 36.11015908967001 21.728801328084366 32.075141343298796 72-73 9.965985399436734 35.839812977012386 22.053307505436845 32.14089411811404 74-75 9.936620726660417 36.26781663959422 22.19156505780165 31.603997575943716 76-77 9.90945490114422 36.12578446992647 21.83989672664447 32.12486390228484 78-79 9.840453753435687 35.91124679029477 22.083003601944057 32.16529585432549 80-81 9.846624292305794 36.328179174334416 22.239319977341175 31.585876556018615 82-83 9.87598936595255 36.15059245720243 21.900065486693077 32.07335269015194 84-85 9.796308027657712 35.93539150015446 22.16424190394125 32.104058568246586 86-87 9.992211130304291 36.60373214177189 22.149600936388243 31.254455791535573 88-89 10.292635431614084 36.65830619922279 21.78416688249993 31.264891486663192 90-91 10.483796201787781 36.10820911966533 22.041843064593365 31.366151613953512 92-93 11.416503670715992 36.31307569042091 21.895205973696296 30.375214665166805 94-95 11.925567427825792 36.61182138179318 21.23276895810523 30.229842232275807 96-97 12.635458186678806 36.715503530964924 21.098833964273755 29.550204318082518 98-99 13.247525460836487 36.9911036186865 20.72519412352819 29.03617679694882 100-101 13.851067473818802 36.9286143253447 19.769155571237164 29.451162629599338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3954.0 1 3928.5 2 3131.0 3 1830.5 4 1349.5 5 1412.5 6 1443.0 7 1406.0 8 1336.5 9 1345.5 10 1447.5 11 1538.5 12 1559.5 13 1845.0 14 2251.5 15 2416.5 16 2542.5 17 2963.5 18 3627.0 19 4181.0 20 4788.5 21 5780.0 22 7031.0 23 8986.5 24 11978.5 25 16759.5 26 25375.0 27 41571.0 28 71874.0 29 120698.0 30 196265.0 31 301111.5 32 431312.5 33 578562.0 34 727274.5 35 860337.0 36 961885.5 37 1023923.5 38 1040001.5 39 1008552.0 40 941943.0 41 857029.5 42 760668.5 43 664464.0 44 580574.5 45 507813.5 46 444916.0 47 396161.5 48 356770.5 49 326181.0 50 308828.5 51 292681.0 52 275018.5 53 264631.5 54 259236.0 55 256063.0 56 255062.0 57 253242.5 58 249982.0 59 244796.5 60 236006.5 61 224719.5 62 212658.5 63 197792.0 64 180522.0 65 162981.0 66 144854.5 67 127399.5 68 110597.5 69 93989.0 70 78621.0 71 65304.5 72 53803.0 73 43854.5 74 35798.0 75 29141.0 76 23966.0 77 19983.5 78 16832.5 79 14200.0 80 12195.5 81 10737.0 82 9527.5 83 8297.0 84 7269.5 85 6733.5 86 6293.0 87 6004.5 88 5232.0 89 4481.0 90 4136.5 91 3799.5 92 3420.5 93 2935.0 94 2577.0 95 2279.0 96 2080.0 97 1963.5 98 1920.5 99 1910.0 100 1910.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.029060153072330827 3 0.0 4 6.909621522340377E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.16344000066615E-4 26-27 0.0 28-29 9.491044629928984E-6 30-31 0.001195202046845326 32-33 0.0 34-35 4.237637401808286E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.7514928151656444E-4 48-49 0.0 50-51 7.508869770798607E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.701775798445847E-5 90-91 0.0 92-93 0.0018230412403794742 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 7017.0 5-9 15018.0 10-14 11673.0 15-19 13256.0 20-24 26651.0 25-29 68414.0 30-34 98352.0 35-39 111827.0 40-44 117543.0 45-49 124294.0 50-54 130962.0 55-59 136808.0 60-64 153090.0 65-69 185242.0 70-74 251626.0 75-79 320194.0 80-84 421348.0 85-89 142574.0 90-94 209796.0 95-99 438951.0 100-102 1.2937525E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.69017235589055 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66933643882327 63.399256600404605 2 12.064066672100921 18.50390704792749 3 3.48496617086017 8.017879688931432 4 1.0547118599959784 3.23544137315574 5 0.3388961585966111 1.2995002406761662 6 0.12222887646187316 0.5624252161636744 7 0.04424634010309898 0.2375281614036809 8 0.03057259415762657 0.1875694012252059 9 0.019933814502289583 0.13758549029018446 >10 0.14807295313900048 2.892680856581232 >50 0.0205663011776119 1.0022850994118135 >100 0.0021808949137134075 0.2895536239720558 >500 4.853301049846238E-5 0.03057741280913417 >1k 1.7239215729050587E-4 0.20380978704760178 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.08724914915758E-4 0.0 0.0 2 5.150054694208907E-4 0.0 0.0 3 5.212860239260236E-4 1.256110901026563E-5 6.280554505132815E-6 4 5.338471329362892E-4 1.256110901026563E-5 6.280554505132815E-6 5 5.338471329362892E-4 1.256110901026563E-5 6.280554505132815E-6 6 5.338471329362892E-4 1.256110901026563E-5 6.280554505132815E-6 7 5.338471329362892E-4 1.256110901026563E-5 6.280554505132815E-6 8 5.526887964516877E-4 1.256110901026563E-5 6.280554505132815E-6 9 5.589693509568204E-4 1.8841663515398442E-5 6.280554505132815E-6 10-11 5.715304599670861E-4 1.8841663515398442E-5 6.280554505132815E-6 12-13 5.93512400735051E-4 1.8841663515398442E-5 6.280554505132815E-6 14-15 6.217748960081486E-4 1.8841663515398442E-5 6.280554505132815E-6 16-17 6.374762822709807E-4 3.7683327030796884E-5 6.280554505132815E-6 18-19 6.751596093017775E-4 5.338471329362892E-5 6.280554505132815E-6 20-21 7.191234908377073E-4 6.908609955646096E-5 6.280554505132815E-6 22-23 7.787887586364691E-4 6.908609955646096E-5 6.280554505132815E-6 24-25 8.415943036877971E-4 6.908609955646096E-5 6.280554505132815E-6 26-27 9.138206804968246E-4 6.908609955646096E-5 6.280554505132815E-6 28-29 0.0010111692753263831 6.908609955646096E-5 6.280554505132815E-6 30-31 0.0011210789791662075 6.908609955646096E-5 6.280554505132815E-6 32-33 0.001234128960258598 7.222637680902736E-5 6.280554505132815E-6 34-35 0.0013094956143201919 8.164720856672659E-5 6.280554505132815E-6 36-37 0.0014099844864023168 8.164720856672659E-5 6.280554505132815E-6 38-39 0.0015387358537575398 8.164720856672659E-5 6.280554505132815E-6 40-41 0.0016800483301230278 8.4787485819293E-5 6.280554505132815E-6 42-43 0.0018370621927513483 9.106804032442581E-5 9.420831757699223E-6 44-45 0.0019752343918642704 9.420831757699221E-5 1.256110901026563E-5 46-47 0.0021636510270182548 9.420831757699221E-5 1.256110901026563E-5 48-49 0.0023395065531619736 1.0048887208212504E-4 1.256110901026563E-5 50-51 0.002559325960841622 1.0048887208212504E-4 1.256110901026563E-5 52-53 0.0027948467547841025 1.0048887208212504E-4 1.256110901026563E-5 54-55 0.0030900328165253447 1.0048887208212504E-4 1.5701386262832037E-5 56-57 0.003363236937498622 1.0048887208212504E-4 2.512221802053126E-5 58-59 0.0037651924258271224 1.0048887208212504E-4 2.512221802053126E-5 60-61 0.004094921537346596 1.0048887208212504E-4 2.512221802053126E-5 62-63 0.004525139520948193 1.0048887208212504E-4 2.512221802053126E-5 64-65 0.004883131127740763 1.0048887208212504E-4 3.1402772525664073E-5 66-67 0.005347892161120592 1.0048887208212504E-4 3.1402772525664073E-5 68-69 0.00581265319450042 1.0048887208212504E-4 3.1402772525664073E-5 70-71 0.006757876647522908 1.0362914933469144E-4 3.1402772525664073E-5 72-73 0.00839082081885744 1.0676942658725785E-4 3.454304977823048E-5 74-75 0.011113441196832516 1.0676942658725785E-4 3.7683327030796884E-5 76-77 0.014385610094006712 1.0676942658725785E-4 3.7683327030796884E-5 78-79 0.018656387157497026 1.0676942658725785E-4 3.7683327030796884E-5 80-81 0.023768758524675138 1.0676942658725785E-4 4.39638815359297E-5 82-83 0.02962223532345892 1.0676942658725785E-4 5.024443604106252E-5 84-85 0.0367381035777744 1.0676942658725785E-4 5.024443604106252E-5 86-87 0.7918366106208824 1.0676942658725785E-4 5.024443604106252E-5 88-89 1.9334530030188741 1.1619025834495706E-4 5.024443604106252E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATAAAT 128925 0.0 68.69341 1 ATAAATC 135955 0.0 66.113014 2 TAAATCA 147055 0.0 61.516075 3 TCATAAA 164930 0.0 55.71651 7 AATCATA 169385 0.0 54.34372 5 AAATCAT 177150 0.0 51.10928 4 ATCATAA 182420 0.0 50.91544 6 AGAGCAC 18305 0.0 48.496613 94-95 CATAAAC 208185 0.0 44.358974 8 ATAAACA 222265 0.0 41.56072 9 GAGCACA 10510 0.0 39.87396 94-95 AAGAGCA 28550 0.0 33.781258 94-95 GTCCCCA 3085 0.0 28.082272 1 ACAAACG 172975 0.0 27.683413 12-13 GAAGAGC 39005 0.0 26.757391 94-95 AAACGGG 182270 0.0 25.926674 14-15 CCCCCGA 4765 0.0 25.491282 1 CAAACGG 179560 0.0 25.317484 14-15 TGATACG 2315 0.0 25.080057 3 ACGGGCA 197845 0.0 23.886263 16-17 >>END_MODULE