##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 20150414_trimmed_2212_lane2_GCCAAT_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15956775 Sequences flagged as poor quality 0 Sequence length 1-101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.53059888354633 31.0 30.0 34.0 16.0 34.0 2 30.180416535739905 31.0 30.0 34.0 25.0 34.0 3 31.12548981145177 31.0 31.0 34.0 28.0 34.0 4 34.018538441843255 35.0 35.0 37.0 28.0 37.0 5 34.4457048821602 37.0 35.0 37.0 30.0 37.0 6 35.19920542920968 37.0 35.0 37.0 32.0 37.0 7 35.37897219223999 37.0 35.0 37.0 33.0 37.0 8 35.42734748708831 37.0 35.0 37.0 33.0 37.0 9 37.10510695559324 39.0 37.0 39.0 33.0 39.0 10-11 37.121056008425526 39.0 37.0 39.0 33.0 39.0 12-13 37.17411259563684 39.0 37.0 39.0 33.5 39.0 14-15 38.40435414018464 40.0 38.0 41.0 33.5 41.0 16-17 38.33940235349821 40.0 38.0 41.0 33.0 41.0 18-19 38.3509925235297 40.0 38.0 41.0 33.5 41.0 20-21 38.34250542462445 40.0 38.0 41.0 33.5 41.0 22-23 38.367088277372915 40.0 38.0 41.0 34.0 41.0 24-25 38.32575659577245 40.0 38.0 41.0 34.0 41.0 26-27 38.221394488956 40.0 38.0 41.0 34.0 41.0 28-29 37.996449347994215 40.0 37.5 41.0 33.0 41.0 30-31 38.133929970046104 40.0 38.0 41.0 33.0 41.0 32-33 37.93872711880463 40.0 37.0 41.0 33.0 41.0 34-35 37.89163330113387 40.0 37.0 41.0 33.0 41.0 36-37 37.87345936350522 40.0 37.0 41.0 33.0 41.0 38-39 37.87720513621278 40.0 37.0 41.0 33.0 41.0 40-41 37.87064921810831 40.0 37.0 41.0 33.0 41.0 42-43 37.85665544250907 40.0 37.0 41.0 33.0 41.0 44-45 37.84647497475519 40.0 37.0 41.0 33.0 41.0 46-47 37.7921475758306 40.0 37.0 41.0 33.0 41.0 48-49 37.77167750484922 40.0 37.0 41.0 33.0 41.0 50-51 37.74371887240763 40.0 37.0 41.0 33.0 41.0 52-53 37.658935112137165 40.0 37.0 41.0 32.0 41.0 54-55 37.59246831415394 40.0 36.5 41.0 32.0 41.0 56-57 37.50306032951382 39.0 36.0 41.0 32.0 41.0 58-59 37.359203307611295 39.0 36.0 41.0 31.0 41.0 60-61 37.20233262146276 39.0 36.0 40.5 31.0 41.0 62-63 36.95854810089834 39.0 35.0 40.0 31.0 41.0 64-65 36.67285788904934 38.0 35.0 40.0 31.0 41.0 66-67 36.25990429327227 37.0 35.0 39.5 31.0 41.0 68-69 35.86141398303822 37.0 34.5 39.0 31.0 40.5 70-71 35.481448340436316 36.0 34.0 39.0 30.0 40.0 72-73 35.09100980530242 36.0 34.0 37.5 30.0 39.0 74-75 34.67942640824555 35.0 34.0 37.0 30.0 39.0 76-77 31.924244229815848 33.0 30.5 34.5 27.0 36.5 78-79 33.89143774107649 35.0 33.0 36.0 29.5 37.0 80-81 33.99701488619597 35.0 34.0 36.0 30.0 37.0 82-83 33.861437903301244 35.0 34.0 35.0 30.0 36.5 84-85 33.71599496834616 35.0 33.0 35.0 30.0 36.0 86-87 33.6370969114393 35.0 33.0 35.0 30.0 36.0 88-89 33.5500496790678 35.0 33.0 35.0 30.0 36.0 90-91 33.52034919992765 35.0 33.0 35.0 30.0 35.0 92-93 33.44609754755098 35.0 33.0 35.0 30.0 35.0 94-95 33.43781459529251 35.0 33.0 35.0 30.0 35.0 96-97 33.48471040690882 35.0 33.0 35.0 30.0 35.0 98-99 33.57920464384846 35.0 33.0 35.0 30.5 35.0 100-101 33.12321670763437 34.5 32.5 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.9457106774171287 1101 2 1.720495461308257 1101 3 1.0571189471726115 1101 4 1.2297542188759607 1101 5 1.3767503036805309 1101 6 0.6697746715820969 1101 7 0.33905665975311194 1101 8 0.22953436287446038 1101 9 0.21080105119801829 1101 10-11 0.17562561401557275 1101 12-13 0.20486121513409472 1101 14-15 0.2104312636951775 1101 16-17 0.19268116541847746 1101 18-19 0.2926752482552857 1101 20-21 0.43845434144587614 1101 22-23 0.44035780427768145 1101 24-25 0.4620587195102175 1101 26-27 0.4681427459086862 1101 28-29 0.48981710637299614 1101 30-31 0.4630513135265417 1101 32-33 0.547046023904727 1101 34-35 0.6000823929861951 1101 36-37 0.5356789491156562 1101 38-39 0.5323481529824505 1101 40-41 0.5524972017824794 1101 42-43 0.59898638177755 1101 44-45 0.5003619441332248 1101 46-47 0.4866079187964587 1101 48-49 0.4947872715078603 1101 50-51 0.5168594643369318 1101 52-53 0.5097955212776384 1101 54-55 0.46494169364208204 1101 56-57 0.4601984084857662 1101 58-59 0.44743632754069296 1101 60-61 0.45989002769479015 1101 62-63 0.5188152065415679 1101 64-65 0.4680215566819257 1101 66-67 0.4189202187384069 1101 68-69 0.4605370264581481 1101 70-71 0.40673273055416814 1101 72-73 0.3920727002182858 1101 74-75 0.3629369260776656 1101 76-77 0.33170678176519175 1101 78-79 0.2847774642206389 1101 80-81 0.2565682176495869 1101 82-83 0.2802995797660728 1101 84-85 0.24668577994500396 1101 86-87 0.319738516517063 1101 88-89 0.28531987847685514 1101 90-91 0.24353776310390884 1101 92-93 0.25312526222704435 1101 94-95 0.23846444384538756 1101 96-97 0.21260920063711808 1101 98-99 0.21685776267934642 1101 100-101 0.2116709676084767 1102 1 1.7780998193926045 1102 2 1.6912261607178323 1102 3 1.0919955456402022 1102 4 0.39849412718973554 1102 5 1.1600188749151243 1102 6 0.5404437688177666 1102 7 0.3091594386214638 1102 8 0.20649613977143133 1102 9 0.2119786403198134 1102 10-11 0.18911559223636942 1102 12-13 0.22497749674873546 1102 14-15 0.24882378856472798 1102 16-17 0.2643484619811147 1102 18-19 0.29891223920784427 1102 20-21 0.40532094598915336 1102 22-23 0.3396613072770478 1102 24-25 0.37254969902745216 1102 26-27 0.3105715596893148 1102 28-29 0.30843872333564093 1102 30-31 0.27566241510108114 1102 32-33 0.3722046683501077 1102 34-35 0.402498878993903 1102 36-37 0.42029250197201407 1102 38-39 0.4560886317246826 1102 40-41 0.4759772344432136 1102 42-43 0.2600602283389932 1102 44-45 0.4086111472352201 1102 46-47 0.4474435363333029 1102 48-49 0.40829484583760234 1102 50-51 0.3566799786774979 1102 52-53 0.3815112181522906 1102 54-55 0.3880451287086899 1102 56-57 0.3584501648894971 1102 58-59 0.2819417852347925 1102 60-61 0.3332744074177114 1102 62-63 0.2932202822726282 1102 64-65 0.32792134837393405 1102 66-67 0.36451366758061 1102 68-69 0.36561632903596575 1102 70-71 0.3035263264668089 1102 72-73 0.27083026436218205 1102 74-75 0.24256206542890624 1102 76-77 0.05375350645391208 1102 78-79 0.2412656687557515 1102 80-81 0.22866421164093254 1102 82-83 0.22271605609171274 1102 84-85 0.22217445577275896 1102 86-87 0.23173198037473952 1102 88-89 0.2036351036139692 1102 90-91 0.19454299947020814 1102 92-93 0.22267133839827125 1102 94-95 0.2092519425604209 1102 96-97 0.2018838416006048 1102 98-99 0.16949377304560898 1102 100-101 0.1853097030836537 1103 1 1.7940312057905707 1103 2 1.6316030336297729 1103 3 0.9666351178617987 1103 4 0.5866409460907178 1103 5 1.1529479780288128 1103 6 0.5006907415979143 1103 7 0.29055947608812716 1103 8 0.20677183851518777 1103 9 0.18768433239114302 1103 10-11 0.1677095681402676 1103 12-13 0.1756952728093779 1103 14-15 0.24994929930361565 1103 16-17 0.2276150970973987 1103 18-19 0.23364829209942428 1103 20-21 0.34335370910579144 1103 22-23 0.14882756413241793 1103 24-25 0.31567976009853993 1103 26-27 0.3386865353360804 1103 28-29 0.3420702924304351 1103 30-31 0.38870734091125314 1103 32-33 0.4126035041524787 1103 34-35 0.30747497149221914 1103 36-37 0.3559390180384341 1103 38-39 0.3702452933729461 1103 40-41 0.36630444559564523 1103 42-43 0.3782705871649199 1103 44-45 0.422625112467351 1103 46-47 0.3927273493636321 1103 48-49 0.265165167965705 1103 50-51 0.3741099726734518 1103 52-53 0.27189516112778733 1103 54-55 0.30148280560784 1103 56-57 0.3204698700364972 1103 58-59 0.36513325309624634 1103 60-61 0.34895939383082464 1103 62-63 0.3598918801646107 1103 64-65 0.3820061275021942 1103 66-67 0.35313239710099964 1103 68-69 0.29565712596783555 1103 70-71 0.27956071978429975 1103 72-73 0.24078438903335808 1103 74-75 0.21393576335862718 1103 76-77 0.06017571730285454 1103 78-79 0.22735285691843643 1103 80-81 0.2208707940211525 1103 82-83 0.21439004669464623 1103 84-85 0.22294025488578484 1103 86-87 0.20020826764734778 1103 88-89 0.19345882618219434 1103 90-91 0.21838106653475364 1103 92-93 0.20024046645454519 1103 94-95 0.22930796849725255 1103 96-97 0.12501306688319147 1103 98-99 0.21871798493543082 1103 100-101 0.16698841743090753 1202 1 1.4717784371220404 1202 2 1.4329214629743063 1202 3 0.6811006264731283 1202 4 -0.055968239448539236 1202 5 0.9316374073118325 1202 6 0.4203840527056357 1202 7 0.20649245592751697 1202 8 0.1379363338409476 1202 9 0.130971889506732 1202 10-11 0.16344861255639387 1202 12-13 0.12722534308714728 1202 14-15 0.12427610503112163 1202 16-17 0.15203406722177704 1202 18-19 0.23844203626725147 1202 20-21 0.27992431579976795 1202 22-23 0.19195033883487156 1202 24-25 0.294033487662702 1202 26-27 0.2517592919748566 1202 28-29 0.24349221205614668 1202 30-31 0.24194868549842852 1202 32-33 0.20186752014730303 1202 34-35 0.23732682467149857 1202 36-37 0.23150589955386636 1202 38-39 0.20993583247667402 1202 40-41 0.10431127774418059 1202 42-43 0.12442400687302779 1202 44-45 0.17956466312150354 1202 46-47 0.2148025989245781 1202 48-49 0.24003314102911588 1202 50-51 0.2664582675811644 1202 52-53 0.27628722106508263 1202 54-55 0.30306464710368886 1202 56-57 0.2552715131371244 1202 58-59 0.2454245445491523 1202 60-61 0.08733996988525661 1202 62-63 0.20709564642416467 1202 64-65 0.23994175622783587 1202 66-67 0.18107143054658792 1202 68-69 0.18434723589519564 1202 70-71 0.15850484653872599 1202 72-73 0.18515242965879253 1202 74-75 0.20659437025265248 1202 76-77 0.03783169643101303 1202 78-79 0.13320209149058115 1202 80-81 0.1844051515184475 1202 82-83 0.13488903198053492 1202 84-85 0.17692604806331502 1202 86-87 0.13327628756033505 1202 88-89 0.15775985814410376 1202 90-91 0.14093029777186672 1202 92-93 0.13618859347027978 1202 94-95 0.07729554782007142 1202 96-97 0.08074740863848007 1202 98-99 0.0972056778844248 1202 100-101 0.12103101251387471 1203 1 0.6388251861979839 1203 2 0.9585494318586143 1203 3 0.38928886047898104 1203 4 1.034782872081287 1203 5 1.1606864007819766 1203 6 0.4379170088314055 1203 7 0.3045326169991469 1203 8 0.1783545282087715 1203 9 0.1474966238001798 1203 10-11 0.15252469271253233 1203 12-13 0.08423257605679169 1203 14-15 0.1535677412193266 1203 16-17 0.121832576644195 1203 18-19 0.17615693101202368 1203 20-21 0.19084505954835862 1203 22-23 0.15265175552750065 1203 24-25 0.16847714407564496 1203 26-27 0.10218872312701421 1203 28-29 0.16399824358281023 1203 30-31 0.1873176673097845 1203 32-33 0.1628538563626094 1203 34-35 0.2096620694724578 1203 36-37 0.22995278043175205 1203 38-39 0.21359160800730592 1203 40-41 0.1656823286525082 1203 42-43 0.18292907118866708 1203 44-45 0.19085170462154366 1203 46-47 0.2645876900365849 1203 48-49 0.24517936403115215 1203 50-51 0.1388882676411498 1203 52-53 0.07773322606301036 1203 54-55 0.21831812776904513 1203 56-57 0.13831239152111152 1203 58-59 0.13682320062397224 1203 60-61 0.18430390820252285 1203 62-63 0.1226400081349226 1203 64-65 0.15155019066339293 1203 66-67 0.23394766133854716 1203 68-69 0.14720683458889283 1203 70-71 0.12632101294956044 1203 72-73 0.1475948446660098 1203 74-75 0.13209510528383817 1203 76-77 -0.05492625878851598 1203 78-79 0.08874249161504366 1203 80-81 0.1437369617309514 1203 82-83 0.14214388453754623 1203 84-85 0.14271218744997327 1203 86-87 0.1044988672529783 1203 88-89 0.08202673050774223 1203 90-91 0.07317527659210299 1203 92-93 0.04700505590054149 1203 94-95 0.057609864573876735 1203 96-97 0.0357535359879364 1203 98-99 0.0934771760784443 1203 100-101 0.09543317692357078 1116 1 1.5383612272707055 1116 2 1.538884867390923 1116 3 0.9384044134760359 1116 4 1.329204718801904 1116 5 1.2939131896994525 1116 6 0.5238954378647236 1116 7 0.2710780088542677 1116 8 0.16127488363454745 1116 9 0.13596324117878567 1116 10-11 0.05877170755442762 1116 12-13 0.06609494006782057 1116 14-15 -0.002080238760946429 1116 16-17 0.07486195116250371 1116 18-19 0.06737699741446335 1116 20-21 0.056739963841074825 1116 22-23 0.2014523518906728 1116 24-25 0.04126572993472877 1116 26-27 0.1406270480337568 1116 28-29 0.15652196477401503 1116 30-31 0.10750201264572468 1116 32-33 0.08183752215381901 1116 34-35 0.19005384845077344 1116 36-37 0.11858094013666687 1116 38-39 0.024540206066959058 1116 40-41 0.11220473120267371 1116 42-43 0.16475515635980997 1116 44-45 0.16609510874409494 1116 46-47 0.07676768446417981 1116 48-49 0.05207412913411957 1116 50-51 0.07712677432733983 1116 52-53 0.1241595229854866 1116 54-55 0.17316056032163374 1116 56-57 0.15560288175927894 1116 58-59 0.11796636167026975 1116 60-61 0.14313579626348627 1116 62-63 0.13522093694837167 1116 64-65 0.13550451671147812 1116 66-67 0.1638612140405158 1116 68-69 0.17173555359345727 1116 70-71 0.04855638693689457 1116 72-73 0.16225166756564136 1116 74-75 0.18618925419747967 1116 76-77 0.2568855797719962 1116 78-79 0.22072277774734061 1116 80-81 0.2274956952878 1116 82-83 0.13185100676604122 1116 84-85 0.1476824107028989 1116 86-87 0.13340639327949333 1116 88-89 0.11358327701800874 1116 90-91 0.10173908082411032 1116 92-93 0.07753063469869659 1116 94-95 0.12099488599587715 1116 96-97 0.1484192349754494 1116 98-99 0.11190000303692926 1116 100-101 0.1576278782148961 1201 1 0.6925985785529782 1201 2 1.1742392226509857 1201 3 0.765001094346097 1201 4 1.077799511464768 1201 5 1.2115478763605267 1201 6 0.4915159759350658 1201 7 0.15246890870790253 1201 8 0.07105397050042939 1201 9 0.04411874300507179 1201 10-11 0.06991851593704723 1201 12-13 0.06919161293387788 1201 14-15 0.023578462316258708 1201 16-17 0.08436337462876509 1201 18-19 0.03911721350191755 1201 20-21 0.04440384058194269 1201 22-23 0.08940120817464958 1201 24-25 0.04053434249001242 1201 26-27 0.0986524862441982 1201 28-29 0.1313612029974962 1201 30-31 0.084443734368989 1201 32-33 0.16239450590426685 1201 34-35 0.15510021946023755 1201 36-37 0.12931600794698284 1201 38-39 0.1444019898729536 1201 40-41 0.1399063289728275 1201 42-43 0.08191612723016561 1201 44-45 0.07530278088834308 1201 46-47 0.10261428964418684 1201 48-49 0.004490882086756187 1201 50-51 0.0840865349238129 1201 52-53 0.07823841494505501 1201 54-55 0.036537509383904876 1201 56-57 0.07689626927034254 1201 58-59 0.11722397739794133 1201 60-61 0.1283865942986253 1201 62-63 0.1362941354593019 1201 64-65 0.11824416643923996 1201 66-67 0.13537662136481288 1201 68-69 0.183460698193052 1201 70-71 0.17821507073630016 1201 72-73 0.1750311825556068 1201 74-75 0.11719480595420606 1201 76-77 0.2255906508618324 1201 78-79 0.11572897558741602 1201 80-81 0.12261873032213089 1201 82-83 0.14175513373641024 1201 84-85 0.12407536993949009 1201 86-87 0.1300734906094263 1201 88-89 0.08616889839353092 1201 90-91 0.08947142021170151 1201 92-93 0.1355545658128392 1201 94-95 0.14469329985537627 1201 96-97 0.08790954378918059 1201 98-99 0.05751968790544737 1201 100-101 0.037624380668546564 1113 1 -0.22131708546971396 1113 2 0.5475286099912218 1113 3 0.17749908558810645 1113 4 0.07339030819070302 1113 5 0.7939151728448053 1113 6 0.2827955676309415 1113 7 0.10713409119579609 1113 8 0.05702417892968725 1113 9 0.08275979732808736 1113 10-11 -0.02236397732622919 1113 12-13 -0.041767093779014886 1113 14-15 -0.07375888639540307 1113 16-17 -0.028050640474056365 1113 18-19 -0.04803754936422422 1113 20-21 -0.08099049774484257 1113 22-23 -6.938053570166858E-4 1113 24-25 0.04089063896989131 1113 26-27 0.05196378930756396 1113 28-29 0.03128302561542995 1113 30-31 0.05526162499273113 1113 32-33 -0.032202203918309635 1113 34-35 -0.004731886693349452 1113 36-37 -0.01838131971587842 1113 38-39 -0.03499040415941579 1113 40-41 -0.08633900320769072 1113 42-43 -0.10024964343149634 1113 44-45 -0.003873380391958392 1113 46-47 -0.09651426540740005 1113 48-49 -0.023368929354830925 1113 50-51 -0.07755355089646798 1113 52-53 -0.041578523089718544 1113 54-55 -0.07036847175985628 1113 56-57 -0.0808576710822635 1113 58-59 -0.04831162246927789 1113 60-61 -0.015949899401810796 1113 62-63 -0.12161924451277173 1113 64-65 -0.14371464002248047 1113 66-67 -0.13362313152169492 1113 68-69 -0.1215713450035949 1113 70-71 -0.12159875729642522 1113 72-73 -0.02169840499326625 1113 74-75 -0.053855389151102884 1113 76-77 -0.05045069316242845 1113 78-79 -0.052695079227532915 1113 80-81 -0.09516595634156033 1113 82-83 -0.09568905042760889 1113 84-85 -0.05232454111282436 1113 86-87 -0.10801192290951178 1113 88-89 -0.05574545805346531 1113 90-91 -0.10377643743677112 1113 92-93 -0.049578670327960594 1113 94-95 -0.03771161156008418 1113 96-97 -0.01542446767983563 1113 98-99 -0.02947251758792646 1113 100-101 -0.014959492629465387 1112 1 -0.15367123889675227 1112 2 0.3253559910125752 1112 3 -0.20527676429246355 1112 4 -0.12038945262727907 1112 5 -0.09499142512305525 1112 6 -0.030959390367954143 1112 7 -0.027104826332909226 1112 8 0.014426290674656173 1112 9 0.03951715426371294 1112 10-11 0.05806705952262092 1112 12-13 0.07939226383204812 1112 14-15 -0.011192792962106068 1112 16-17 -1.0966536159884299E-4 1112 18-19 -0.01811479699312457 1112 20-21 -0.00882094591973015 1112 22-23 -0.032334648033419455 1112 24-25 -0.045884370968316546 1112 26-27 -0.05308616087028639 1112 28-29 -0.03223642432902807 1112 30-31 -0.07095792199584139 1112 32-33 -0.10206289920542844 1112 34-35 -0.08389610274814174 1112 36-37 -0.10096445008876742 1112 38-39 -0.01885728790168173 1112 40-41 -0.03247015505270667 1112 42-43 -0.10143409917250068 1112 44-45 -0.09647417129558988 1112 46-47 -0.0675434943380182 1112 48-49 -0.03093971019602293 1112 50-51 -0.031279333247233865 1112 52-53 -0.07876608990064682 1112 54-55 -0.02015916451721722 1112 56-57 -0.04510949761774441 1112 58-59 -0.07659861598642692 1112 60-61 -0.026180632831319883 1112 62-63 -0.0012721125096248898 1112 64-65 -0.03594852135815074 1112 66-67 0.004791282575247635 1112 68-69 -0.029904739216696896 1112 70-71 0.003605128395371082 1112 72-73 0.004315160615306013 1112 74-75 0.010559966540242272 1112 76-77 0.10681097824156893 1112 78-79 0.01199838791918495 1112 80-81 0.02737094903098125 1112 82-83 0.010997777336946513 1112 84-85 -0.031033219735732587 1112 86-87 0.0062271640325874955 1112 88-89 0.006458045579933014 1112 90-91 0.03095518058749036 1112 92-93 0.028396009054311833 1112 94-95 0.05704233460727437 1112 96-97 0.03109703346751047 1112 98-99 0.02465216875663856 1112 100-101 -0.051188071441806926 1204 1 -1.3329399928787744 1204 2 -0.9634713173923153 1204 3 -0.7063941079665064 1204 4 0.46333986818468986 1204 5 -0.27655340832976094 1204 6 -0.06671763607378978 1204 7 -0.06851061545287251 1204 8 -0.058077284581820265 1204 9 -0.06331001856427321 1204 10-11 -0.05336946074008608 1204 12-13 -0.055823616224614625 1204 14-15 -0.06936655655043467 1204 16-17 -0.04871235532888818 1204 18-19 -0.0919758213032722 1204 20-21 -0.16415332380311298 1204 22-23 -0.1933840114784573 1204 24-25 -0.1973565171126168 1204 26-27 -0.2506441342034904 1204 28-29 -0.1597149728682723 1204 30-31 -0.1787165118421825 1204 32-33 -0.18082948124601472 1204 34-35 -0.18775499393191097 1204 36-37 -0.2757832494310435 1204 38-39 -0.19179563013838674 1204 40-41 -0.21908870697181015 1204 42-43 -0.15966174937265976 1204 44-45 -0.15737161694287494 1204 46-47 -0.210516774586182 1204 48-49 -0.1531733638428605 1204 50-51 -0.14614704501889975 1204 52-53 -0.1124128698868887 1204 54-55 -0.15541705916353266 1204 56-57 -0.1498711463283513 1204 58-59 -0.10635065883435857 1204 60-61 -0.16913060699902616 1204 62-63 -0.18483796845828238 1204 64-65 -0.13553704212444018 1204 66-67 -0.20932900447909475 1204 68-69 -0.1783629705387213 1204 70-71 -0.1639027999311793 1204 72-73 -0.1565625743979453 1204 74-75 -0.07728015729197324 1204 76-77 -0.1466785633449561 1204 78-79 -0.14411216426758244 1204 80-81 -0.09649412950533076 1204 82-83 -0.08905109121448618 1204 84-85 -0.04615354413937922 1204 86-87 -0.06696548708841021 1204 88-89 -0.0502847842183769 1204 90-91 -0.06490343697662126 1204 92-93 -0.06099696037784241 1204 94-95 -0.03007000307532337 1204 96-97 -0.04241725057792678 1204 98-99 -0.0633308705710931 1204 100-101 -0.03298717122157058 1115 1 -1.9292724288015926 1115 2 -1.8975386491179194 1115 3 -1.2644007643000563 1115 4 -0.6770975780700965 1115 5 -1.372343744585237 1115 6 -0.6264886626019575 1115 7 -0.35188338369493266 1115 8 -0.1786474319029736 1115 9 -0.17327331865407558 1115 10-11 -0.17526260188311937 1115 12-13 -0.16582765620245254 1115 14-15 -0.16828020193591442 1115 16-17 -0.2896050452938752 1115 18-19 -0.22784451186415566 1115 20-21 -0.27840990023587864 1115 22-23 -0.347751912154564 1115 24-25 -0.26728838985266634 1115 26-27 -0.3289142002408596 1115 28-29 -0.3703919010986212 1115 30-31 -0.3433255890080318 1115 32-33 -0.35037668951569145 1115 34-35 -0.41508644145879003 1115 36-37 -0.32882386664633145 1115 38-39 -0.39984150838532173 1115 40-41 -0.31575079322078636 1115 42-43 -0.31653322660670824 1115 44-45 -0.3008289671113218 1115 46-47 -0.32433786316962454 1115 48-49 -0.277155186555035 1115 50-51 -0.3134505515688204 1115 52-53 -0.36513560228664943 1115 54-55 -0.30777526116528264 1115 56-57 -0.3932569128153318 1115 58-59 -0.3078374365637444 1115 60-61 -0.32579103769996465 1115 62-63 -0.24978601190856153 1115 64-65 -0.2625190784472906 1115 66-67 -0.35046343171496375 1115 68-69 -0.2603806184781874 1115 70-71 -0.23840852645186317 1115 72-73 -0.20490837199633205 1115 74-75 -0.2631955715551797 1115 76-77 -0.012063840175656537 1115 78-79 -0.23177885436692947 1115 80-81 -0.26411172779526737 1115 82-83 -0.20739356912885398 1115 84-85 -0.2258807519446293 1115 86-87 -0.23044903745388012 1115 88-89 -0.1781274866258542 1115 90-91 -0.17630578856823576 1115 92-93 -0.1998231736727405 1115 94-95 -0.18271269433893167 1115 96-97 -0.18973776129499242 1115 98-99 -0.15853532915103585 1115 100-101 -0.11575496565436794 1114 1 -2.467604008665795 1114 2 -2.100280225202905 1114 3 -1.4576373439751293 1114 4 -1.2729005498395551 1114 5 -2.073907530235907 1114 6 -0.8895286654463561 1114 7 -0.41934574160557503 1114 8 -0.2342494274803144 1114 9 -0.24746913415699368 1114 10-11 -0.15798756561118665 1114 12-13 -0.24106714153860764 1114 14-15 -0.25691818390740195 1114 16-17 -0.26915064822654244 1114 18-19 -0.20523142600470123 1114 20-21 -0.2977509829761473 1114 22-23 -0.37747447632097675 1114 24-25 -0.3224981344103881 1114 26-27 -0.38528549019945757 1114 28-29 -0.4292648625946711 1114 30-31 -0.41863954429234695 1114 32-33 -0.4316934522450211 1114 34-35 -0.4594767921599754 1114 36-37 -0.4943684195872109 1114 38-39 -0.49579473775112604 1114 40-41 -0.4257337253608853 1114 42-43 -0.37532748766075485 1114 44-45 -0.4573277769474231 1114 46-47 -0.4389210931618166 1114 48-49 -0.4237936825757558 1114 50-51 -0.43076102271663785 1114 52-53 -0.3657684241504242 1114 54-55 -0.5764686405044941 1114 56-57 -0.5548849663680286 1114 58-59 -0.4850045679548032 1114 60-61 -0.4236224208751267 1114 62-63 -0.39717572076256147 1114 64-65 -0.33322218421547944 1114 66-67 -0.42987235016936864 1114 68-69 -0.4771370182021144 1114 70-71 -0.3832686073004936 1114 72-73 -0.37538012763845785 1114 74-75 -0.32048625010892096 1114 76-77 -0.060961803080882504 1114 78-79 -0.2769364300681687 1114 80-81 -0.32727623256830185 1114 82-83 -0.3299300466479025 1114 84-85 -0.3136680462072974 1114 86-87 -0.26041730983141775 1114 88-89 -0.22090703219553376 1114 90-91 -0.24103790249338175 1114 92-93 -0.25311161457734244 1114 94-95 -0.17979545199071367 1114 96-97 -0.14369988936250877 1114 98-99 -0.2160342001058453 1114 100-101 -0.20073397414248717 1109 1 -1.761358412926647 1109 2 -2.4033750509217384 1109 3 -1.786817671227201 1109 4 -1.121803514079403 1109 5 -1.8446336052503298 1109 6 -0.7739781456895187 1109 7 -0.38013396831981083 1109 8 -0.19542593966676236 1109 9 -0.19849903400859148 1109 10-11 -0.24205397218334213 1109 12-13 -0.19172359351446744 1109 14-15 -0.2058163256847152 1109 16-17 -0.24780415793516397 1109 18-19 -0.28887207027068484 1109 20-21 -0.36422043754618016 1109 22-23 -0.3447677381585379 1109 24-25 -0.3816347964620306 1109 26-27 -0.400812793028237 1109 28-29 -0.43954069511951843 1109 30-31 -0.42038236750978086 1109 32-33 -0.464897800341042 1109 34-35 -0.4943952841988306 1109 36-37 -0.4920653148102474 1109 38-39 -0.4370626324976641 1109 40-41 -0.44592674241036434 1109 42-43 -0.5186814429560229 1109 44-45 -0.4400407600093459 1109 46-47 -0.3777229325277176 1109 48-49 -0.4007895016480916 1109 50-51 -0.4571836104396496 1109 52-53 -0.44604839670818563 1109 54-55 -0.39087850445147154 1109 56-57 -0.3620487214374748 1109 58-59 -0.34331758891480035 1109 60-61 -0.3438568873392569 1109 62-63 -0.3679794442399924 1109 64-65 -0.4073583792805664 1109 66-67 -0.3351212851585572 1109 68-69 -0.32320304150149326 1109 70-71 -0.28474048185557876 1109 72-73 -0.3405000088953827 1109 74-75 -0.3836989325296756 1109 76-77 0.08259677690916334 1109 78-79 -0.25731361572083955 1109 80-81 -0.2938599494673255 1109 82-83 -0.2373217791560478 1109 84-85 -0.2748478585759955 1109 86-87 -0.24225618976228702 1109 88-89 -0.22274893809007779 1109 90-91 -0.24512085255512517 1109 92-93 -0.23366909671939595 1109 94-95 -0.19248768111694403 1109 96-97 -0.1985270242401569 1109 98-99 -0.21264313252817857 1109 100-101 -0.1277492764357291 1108 1 -2.3901309718620816 1108 2 -2.8624338736006862 1108 3 -1.0822414675426089 1108 4 -0.612323142507968 1108 5 -1.8217226378026155 1108 6 -0.8195630480101741 1108 7 -0.48260668531088413 1108 8 -0.2686473872240498 1108 9 -0.3544492722276047 1108 10-11 -0.31927214562212924 1108 12-13 -0.2809529963683417 1108 14-15 -0.2767099093524479 1108 16-17 -0.30192091126831144 1108 18-19 -0.4591036646660527 1108 20-21 -0.5491464294110173 1108 22-23 -0.4091485926325902 1108 24-25 -0.467204952841513 1108 26-27 -0.4845922313791604 1108 28-29 -0.5173076590113652 1108 30-31 -0.5151576204491022 1108 32-33 -0.566054836566714 1108 34-35 -0.55820592326954 1108 36-37 -0.5753195477076503 1108 38-39 -0.5232498775873609 1108 40-41 -0.47680973018416495 1108 42-43 -0.5392629843256671 1108 44-45 -0.512285177748808 1108 46-47 -0.5167985434612348 1108 48-49 -0.5368012524029808 1108 50-51 -0.45157219290321393 1108 52-53 -0.4790995309587416 1108 54-55 -0.43017392033794266 1108 56-57 -0.42002719963623747 1108 58-59 -0.48252663688221276 1108 60-61 -0.4687904981380129 1108 62-63 -0.45691074165751644 1108 64-65 -0.46270358465565664 1108 66-67 -0.412334522642702 1108 68-69 -0.478209678419951 1108 70-71 -0.4612452029459888 1108 72-73 -0.4394704204824009 1108 74-75 -0.41390004026013827 1108 76-77 -0.3806178447591009 1108 78-79 -0.3651391662781336 1108 80-81 -0.32459912141644764 1108 82-83 -0.3202205151141442 1108 84-85 -0.34066533609879457 1108 86-87 -0.3064929488221324 1108 88-89 -0.2669196801041167 1108 90-91 -0.22378186677578782 1108 92-93 -0.2906159111098603 1108 94-95 -0.2923580177081533 1108 96-97 -0.2811079762488333 1108 98-99 -0.2692830691472068 1108 100-101 -0.23640062571524822 1111 1 -2.9737176474882254 1111 2 -2.9258037110978243 1111 3 -1.1859347372682905 1111 4 -2.8586137814919823 1111 5 -2.9757884323647126 1111 6 -1.4160030910452264 1111 7 -0.7429969143526591 1111 8 -0.4323504521792856 1111 9 -0.40686188763600484 1111 10-11 -0.34597060629040755 1111 12-13 -0.35408077690557604 1111 14-15 -0.3489463803525652 1111 16-17 -0.3670802976367611 1111 18-19 -0.5208828962484588 1111 20-21 -0.5622659392234013 1111 22-23 -0.4800188357142616 1111 24-25 -0.5629634112052742 1111 26-27 -0.5844142576741405 1111 28-29 -0.5504075727598803 1111 30-31 -0.5069962401515937 1111 32-33 -0.6302205797847193 1111 34-35 -0.7310657001083172 1111 36-37 -0.6305898396404856 1111 38-39 -0.6631795629908837 1111 40-41 -0.7415302934602934 1111 42-43 -0.6110936276461274 1111 44-45 -0.6302495229002218 1111 46-47 -0.6093858168048314 1111 48-49 -0.5957249089828593 1111 50-51 -0.5579686109821509 1111 52-53 -0.6463086005091228 1111 54-55 -0.606772804721821 1111 56-57 -0.5100742876998652 1111 58-59 -0.5469699856263688 1111 60-61 -0.6073741640646872 1111 62-63 -0.5671683936203493 1111 64-65 -0.4926618061376047 1111 66-67 -0.5123975387718147 1111 68-69 -0.5070064486815156 1111 70-71 -0.4350425712521684 1111 72-73 -0.45645435103727294 1111 74-75 -0.45908912701526816 1111 76-77 -0.43425646224195447 1111 78-79 -0.4994125640083169 1111 80-81 -0.46225040036893006 1111 82-83 -0.37202293363347394 1111 84-85 -0.4678364049397814 1111 86-87 -0.42516594608751035 1111 88-89 -0.44700838500459383 1111 90-91 -0.35182945568172386 1111 92-93 -0.32719103825004225 1111 94-95 -0.37134588610783226 1111 96-97 -0.38487259816427866 1111 98-99 -0.33602084103406327 1111 100-101 -0.3210752081196162 1110 1 -3.0591795703461386 1110 2 -4.006770183683116 1110 3 -1.6860468532340853 1110 4 0.5491131901507416 1110 5 -2.4614931173651406 1110 6 -1.1589900788029723 1110 7 -0.6508293292711258 1110 8 -0.6417666676811393 1110 9 -0.5989409696589689 1110 10-11 -0.5410736014397202 1110 12-13 -0.518372164225525 1110 14-15 -0.5780349749559193 1110 16-17 -0.6837874491780411 1110 18-19 -0.7947681467488152 1110 20-21 -0.9422682323180496 1110 22-23 -0.7913227008006274 1110 24-25 -0.7340934552190603 1110 26-27 -0.8242660387173615 1110 28-29 -0.8285929268747836 1110 30-31 -0.7011285759822243 1110 32-33 -0.7834271533864694 1110 34-35 -0.8209488705903851 1110 36-37 -0.761185865924034 1110 38-39 -0.8756608264557997 1110 40-41 -0.7675349911758573 1110 42-43 -0.7228675129867597 1110 44-45 -0.7967363276282029 1110 46-47 -0.8807171578362585 1110 48-49 -0.7829472228145775 1110 50-51 -0.7928024181930766 1110 52-53 -0.7927160448161814 1110 54-55 -0.7539295472401832 1110 56-57 -0.6919450996429362 1110 58-59 -0.837140929198668 1110 60-61 -0.8200867693970011 1110 62-63 -0.7310427215590636 1110 64-65 -0.6613007132516486 1110 66-67 -0.709030554053605 1110 68-69 -0.6651214736707871 1110 70-71 -0.6249062332952278 1110 72-73 -0.5429159932572105 1110 74-75 -0.5804250852865138 1110 76-77 -0.6844981136493047 1110 78-79 -0.5856977873956524 1110 80-81 -0.5136941617448514 1110 82-83 -0.4660778884057777 1110 84-85 -0.4757817774455333 1110 86-87 -0.49200469006906644 1110 88-89 -0.5021200909998953 1110 90-91 -0.45981828263943925 1110 92-93 -0.5528606982997601 1110 94-95 -0.5817414319064653 1110 96-97 -0.4735508822586212 1110 98-99 -0.5058174180907784 1110 100-101 -0.4124291613300457 1105 1 1.920291265651226 1105 2 1.7302045868713591 1105 3 1.0577360214271962 1105 4 0.6555141936922126 1105 5 1.2183839152572702 1105 6 0.6248600325338387 1105 7 0.37210047514231803 1105 8 0.24708268754251606 1105 9 0.22752402644201908 1105 10-11 0.2535230480990833 1105 12-13 0.29321078650325205 1105 14-15 0.3806080425999241 1105 16-17 0.4051324084888748 1105 18-19 0.41137398290464233 1105 20-21 0.5086526582240651 1105 22-23 0.44158544580561454 1105 24-25 0.4042816652524195 1105 26-27 0.5282348772046745 1105 28-29 0.43505636623785904 1105 30-31 0.47667601536080895 1105 32-33 0.5444437163296598 1105 34-35 0.5745016949866226 1105 36-37 0.55751740995278 1105 38-39 0.5811766288991862 1105 40-41 0.5392375447004909 1105 42-43 0.5563500698441501 1105 44-45 0.49696949513793953 1105 46-47 0.5521992120810566 1105 48-49 0.5353544508816412 1105 50-51 0.5036146563471107 1105 52-53 0.5437156731406958 1105 54-55 0.5153390767361046 1105 56-57 0.492828687205801 1105 58-59 0.49833486611166933 1105 60-61 0.48342570032651366 1105 62-63 0.4661389799251836 1105 64-65 0.3582567328473303 1105 66-67 0.39025540933219105 1105 68-69 0.3989784166009329 1105 70-71 0.42871692318743726 1105 72-73 0.3666835006881186 1105 74-75 0.3562621838743283 1105 76-77 0.34958993287582274 1105 78-79 0.37665079359501874 1105 80-81 0.32030283408735727 1105 82-83 0.28412939997960507 1105 84-85 0.2899220121130597 1105 86-87 0.2854725109523102 1105 88-89 0.23348420345730858 1105 90-91 0.2468172739672596 1105 92-93 0.2956897269086767 1105 94-95 0.25693076239257095 1105 96-97 0.2727195724688656 1105 98-99 0.2774623546130144 1105 100-101 0.21627395167156038 1104 1 1.6954389933273966 1104 2 1.5692684596612345 1104 3 1.0290620482869102 1104 4 0.09183562063169148 1104 5 1.0377375961397632 1104 6 0.5160063717479417 1104 7 0.3413718788146056 1104 8 0.1542404358602667 1104 9 0.1742085433481364 1104 10-11 0.1880626260349061 1104 12-13 0.25480703176661024 1104 14-15 0.24164650808943833 1104 16-17 0.19609617590357686 1104 18-19 0.3295449590245738 1104 20-21 0.39580020008571637 1104 22-23 0.3884794982961495 1104 24-25 0.3572028496413111 1104 26-27 0.4081121219056527 1104 28-29 0.4188483645625709 1104 30-31 0.3887266441721593 1104 32-33 0.43543352402215163 1104 34-35 0.4072910614487455 1104 36-37 0.47479333494288767 1104 38-39 0.44496733170444713 1104 40-41 0.3748006947989708 1104 42-43 0.41837637658115057 1104 44-45 0.2980986185801484 1104 46-47 0.34137042205596657 1104 48-49 0.3775134395480535 1104 50-51 0.2703948719803009 1104 52-53 0.3767749406749701 1104 54-55 0.2538083631819248 1104 56-57 0.3040425404969085 1104 58-59 0.3343771137685039 1104 60-61 0.3925952237179686 1104 62-63 0.28470982583769455 1104 64-65 0.3065109488149034 1104 66-67 0.3551192628280333 1104 68-69 0.26595629652710073 1104 70-71 0.23860807213596047 1104 72-73 0.18572454984477815 1104 74-75 0.288920156869203 1104 76-77 -0.03929916215209417 1104 78-79 0.26104172779066914 1104 80-81 0.22161733969440434 1104 82-83 0.17266029708120811 1104 84-85 0.23393452242669355 1104 86-87 0.24167561447179509 1104 88-89 0.2412369530939813 1104 90-91 0.20323561479399643 1104 92-93 0.21518399780751452 1104 94-95 0.1919220830691657 1104 96-97 0.2213828580011068 1104 98-99 0.2121845139230416 1104 100-101 0.11003112554315209 1107 1 1.6025856348946448 1107 2 1.60751248916511 1107 3 1.0494046237131585 1107 4 0.36045941040980267 1107 5 1.07622436445034 1107 6 0.5306617706936976 1107 7 0.2991757277923952 1107 8 0.19998248547844355 1107 9 0.27628139749126746 1107 10-11 0.15189567634029544 1107 12-13 0.18933377348950842 1107 14-15 0.2618418554390729 1107 16-17 0.30026419155299067 1107 18-19 0.2851753724835291 1107 20-21 0.26615556312572863 1107 22-23 0.28342684858157696 1107 24-25 0.3076780227058009 1107 26-27 0.36559248637714603 1107 28-29 0.3883004904617593 1107 30-31 0.3051652136250027 1107 32-33 0.39946186764743885 1107 34-35 0.46932776251978225 1107 36-37 0.3720770566483935 1107 38-39 0.4134617946802095 1107 40-41 0.34184694919712655 1107 42-43 0.40462662631854585 1107 44-45 0.38559262261798466 1107 46-47 0.32474970614128296 1107 48-49 0.2961274817390276 1107 50-51 0.32061050298685245 1107 52-53 0.3671888265669949 1107 54-55 0.38274093146582544 1107 56-57 0.3741510074491998 1107 58-59 0.4130574595726273 1107 60-61 0.3589549074941445 1107 62-63 0.3146668635263694 1107 64-65 0.27513649193282674 1107 66-67 0.31134521347699007 1107 68-69 0.33324047873511375 1107 70-71 0.3087122275589991 1107 72-73 0.2291582528511995 1107 74-75 0.23691876445681004 1107 76-77 0.1332125120530776 1107 78-79 0.267839261700793 1107 80-81 0.2713843698402343 1107 82-83 0.22323609590134197 1107 84-85 0.2318528285427135 1107 86-87 0.20754305728988243 1107 88-89 0.22819638333105274 1107 90-91 0.197595199415737 1107 92-93 0.17512158209788709 1107 94-95 0.15328369813433085 1107 96-97 0.1743786949065509 1107 98-99 0.18290670551236587 1107 100-101 0.17319045797169963 1106 1 1.2114703317184876 1106 2 1.2318832337842451 1106 3 0.17150332534209767 1106 4 -1.1312327276994978 1106 5 0.5076708215863732 1106 6 0.24328331809687143 1106 7 0.1302817264441245 1106 8 0.14498645488514939 1106 9 0.17349819463348837 1106 10-11 0.22869121794683167 1106 12-13 0.08059272632947057 1106 14-15 0.09638138459924761 1106 16-17 0.2169917006036144 1106 18-19 0.28240761129261926 1106 20-21 0.3183760914307996 1106 22-23 0.2991025978523254 1106 24-25 0.17427196870308848 1106 26-27 0.2474836412039636 1106 28-29 0.2182690222289807 1106 30-31 0.18084170371859187 1106 32-33 0.22161838723481964 1106 34-35 0.2022422706768694 1106 36-37 0.25182797481214436 1106 38-39 0.2496749980796693 1106 40-41 0.3384154039543503 1106 42-43 0.2744171424817452 1106 44-45 0.271114503428457 1106 46-47 0.31858753345191104 1106 48-49 0.30567358461195226 1106 50-51 0.3498890444904674 1106 52-53 0.32053435630766103 1106 54-55 0.2745045299410336 1106 56-57 0.2718517683766777 1106 58-59 0.2763391528647219 1106 60-61 0.28051698761446175 1106 62-63 0.23909859399391564 1106 64-65 0.17187211329806473 1106 66-67 0.17983743958887288 1106 68-69 0.23416133811729622 1106 70-71 0.23205373508432103 1106 72-73 0.17829131063884063 1106 74-75 0.1977611909048349 1106 76-77 0.2255986086884718 1106 78-79 0.18376316399237425 1106 80-81 0.15241642438421366 1106 82-83 0.15863856385634278 1106 84-85 0.18928561035830427 1106 86-87 0.13791138203630027 1106 88-89 0.11253369749317699 1106 90-91 0.12619284985400014 1106 92-93 0.18113993050440058 1106 94-95 0.13142594645290728 1106 96-97 0.13742385847110938 1106 98-99 0.12875956984532166 1106 100-101 0.0380968750599493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 49.0 15 2349.0 16 1874.0 17 1940.0 18 1306.0 19 1225.0 20 1461.0 21 1384.0 22 1909.0 23 3056.0 24 6590.0 25 16370.0 26 41904.0 27 92961.0 28 176426.0 29 288254.0 30 420775.0 31 565750.0 32 729343.0 33 934458.0 34 1213501.0 35 1641130.0 36 2332957.0 37 3678613.0 38 3541242.0 39 258929.0 40 1019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.066786678385826 41.75769226551104 13.27233729873361 36.90318375736952 2 8.510325489854822 45.20252239527992 20.545332370003806 25.741819744861456 3 5.908540118240993 18.034092287136286 43.65907211497334 32.39829547964938 4 8.648251577751216 39.40731459817094 12.548858735404744 39.3955750886731 5 9.512897403065725 42.35041259351877 15.42850995385312 32.70818004956238 6 8.262348683189177 46.86578860050557 18.49459664776223 26.377266068543033 7 6.222268181500072 30.102510371565295 38.580178552212516 25.09504289472212 8 6.446383601183949 32.97131829089981 16.4380715257447 44.14422658217154 9 6.07876368091907 56.27786293423179 17.98910977295917 19.654263611889967 10-11 6.05134007648546 45.253501202601775 28.50916212605214 20.185996594860622 12-13 5.170607768724538 57.08243830332304 16.61906175798054 21.127892169971894 14-15 5.8350313439661985 45.716210608232196 16.16802656568818 32.280731482113424 16-17 5.7010351994425 55.41097108410305 16.731675644161655 22.156318072292784 18-19 17.913180968772295 31.02700006975529 17.94068439422175 33.11913456725066 20-21 27.9849870570335 32.008067484630786 17.92466212728812 22.0822833310476 22-23 3.740592609111784 45.288401997228945 17.470317845135234 33.500687548524034 24-25 3.609257008188348 44.69808590593847 16.995886049423373 34.6967710364498 26-27 3.5997199599570324 44.88399004600168 29.047198486425952 22.469091507615342 28-29 3.605149337079504 43.71480451922019 16.94976396647265 35.730282177227664 30-31 3.597466397398742 33.24818552609332 29.09043782978451 34.063910246723424 32-33 3.665611019653001 45.500715652926 21.367199387864726 29.46647393955627 34-35 3.6502940023645007 44.98065509973983 20.99546554646358 30.37358535143209 36-37 3.649025534614951 44.7578983062945 21.216181100576957 30.376895058513586 38-39 3.6617128790366085 44.968389977388526 21.41880915300756 29.951087990567306 40-41 3.660300860182014 44.81111566285026 21.250476941584516 30.27810653538321 42-43 3.639972054608023 44.502690607647956 21.41513301588655 30.442204321857474 44-45 3.6395700168299703 44.812967735679244 21.432326886524912 30.115135360965876 46-47 3.6666135476444235 44.76563514868868 21.190697365877536 30.377053937789363 48-49 3.6247521096123845 44.5555039880702 21.40092419323541 30.41881970908201 50-51 3.6224787057170116 44.73673626320272 21.525830021035116 30.11495501004515 52-53 3.6285987840540637 44.68326144838892 21.3459905606657 30.342149206891317 54-55 3.6039447228398087 44.448358777267224 21.511555064260556 30.43614143563242 56-57 3.60110729910973 44.74371653338907 21.536823910150062 30.118352257351138 58-59 3.61379608465491 44.673171483224856 21.336368341335582 30.376664090784654 60-61 3.5850517900408287 44.47346336256122 21.530447941218707 30.411036906179245 62-63 3.570846752507472 44.669405138972465 21.61873927731135 30.141008831208715 64-65 3.5826275963158545 44.64239799361773 21.4168709691117 30.35810344095472 66-67 3.543904448884245 44.400984059751515 21.573434383942992 30.481677107421252 68-69 3.5364578045034163 44.67422816936095 21.63446452527906 30.154849500856567 70-71 3.5437139648733726 44.66599272239069 21.3936240166458 30.39666929609014 72-73 3.5090261087993264 44.399576172535184 21.60642080125191 30.48497691741358 74-75 3.4949822009819735 44.65156200683029 21.687978166183054 30.165477626004687 76-77 3.47896248537818 44.67826409931045 21.481269488406195 30.361503926905176 78-79 3.451388947392219 44.45388138986033 21.638997982079243 30.455731680668208 80-81 3.44180490172819 44.71943554163642 21.699048018309853 30.139711538325535 82-83 3.4414870656117964 44.726744364978714 21.495105540859193 30.336663028550298 84-85 3.4051878661308295 44.50184313854683 21.666184318858868 30.42678467646347 86-87 3.414345954550204 44.77922181878045 21.735659416138343 30.070772810531 88-89 3.4414560019614258 44.77525707427519 21.53403929042411 30.249247633339273 90-91 3.4442852753898223 44.56360147268249 21.67149877521571 30.32061447671197 92-93 3.538205680924854 44.75313037353316 21.693450612277722 30.015213333264263 94-95 3.5821814094954787 44.805400211492795 21.490422313573728 30.121996065437994 96-97 3.6433433195433054 44.639238516280095 21.604894641767903 30.112523522408697 98-99 3.711391214315602 44.86384867887397 21.63955413849101 29.785205968319424 100-101 3.821139529330465 44.90036481506341 21.289218438961644 29.98927721664448 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3798.0 1 3748.5 2 3168.5 3 2355.5 4 2104.0 5 2189.0 6 2345.0 7 2408.0 8 2332.0 9 2419.0 10 2634.0 11 2809.0 12 2996.0 13 3515.5 14 4492.5 15 5501.5 16 6552.5 17 8146.0 18 10977.0 19 14864.0 20 21060.5 21 33053.5 22 54489.0 23 91556.0 24 150145.5 25 236391.0 26 357183.0 27 515259.0 28 705401.5 29 909380.0 30 1103954.5 31 1259133.0 32 1354668.0 33 1377059.0 34 1321802.0 35 1217351.0 36 1081243.0 37 930855.0 38 781868.0 39 640606.5 40 517918.0 41 417676.5 42 337595.0 43 275019.0 44 229956.0 45 196646.0 46 169676.0 47 149129.0 48 130676.5 49 111601.5 50 98170.0 51 87976.5 52 79504.0 53 74662.5 54 71124.0 55 67691.5 56 64978.5 57 62914.0 58 60753.0 59 58282.5 60 55592.0 61 52643.0 62 49409.5 63 45286.5 64 41010.5 65 37037.5 66 32842.5 67 28924.0 68 25447.0 69 21881.0 70 18325.5 71 15336.5 72 12925.5 73 10919.0 74 9271.5 75 7855.0 76 6775.5 77 6033.0 78 5451.5 79 4877.0 80 4403.0 81 4083.0 82 3803.0 83 3414.5 84 3078.5 85 2958.5 86 2849.5 87 2788.0 88 2376.5 89 1964.0 90 1804.0 91 1651.5 92 1470.5 93 1217.5 94 1069.0 95 976.5 96 924.5 97 893.5 98 881.5 99 880.0 100 882.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04087296429512812 3 0.0 4 9.401627170953233E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.848022349758018E-4 26-27 0.0 28-29 1.8931521940072077E-5 30-31 4.7746797027859914E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.098529382086974E-5 90-91 0.0 92-93 0.0016527914414018827 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 5967.0 5-9 9960.0 10-14 7528.0 15-19 10444.0 20-24 24269.0 25-29 76886.0 30-34 135936.0 35-39 157161.0 40-44 141188.0 45-49 133520.0 50-54 125370.0 55-59 117112.0 60-64 115705.0 65-69 119469.0 70-74 138409.0 75-79 132400.0 80-84 131720.0 85-89 115435.0 90-94 155447.0 95-99 307380.0 100-102 1.3795469E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.58887661113519 #Duplication Level Percentage of deduplicated Percentage of total 1 94.90109045673117 85.02082083190268 2 3.6761301628387373 6.58680743130064 3 0.7335535467644211 1.9715471456621496 4 0.2647028495027866 0.9485772369088417 5 0.10869476691120791 0.4868921030542154 6 0.11847916010451355 0.6368648913356517 7 0.03385968562944396 0.2123415838563658 8 0.020918584284029526 0.14992579730412425 9 0.015499136447597718 0.12496952005046824 >10 0.11267149615530501 2.55034217151006 >50 0.012942738576711502 0.7480645395763816 >100 0.00119967596091686 0.18331220621882796 >500 1.3293394829757082E-4 0.07249834627179753 >1k 1.0284580314397915E-4 0.15331113771181804 >5k 1.4958153816349418E-5 0.07354125115410559 >10k+ 7.002187946505973E-6 0.08018380618192969 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.452229538863586E-4 0.0 6.266930504440903E-6 2 5.514898843907995E-4 0.0 6.266930504440903E-6 3 5.640237453996813E-4 0.0 6.266930504440903E-6 4 5.640237453996813E-4 0.0 6.266930504440903E-6 5 5.640237453996813E-4 0.0 6.266930504440903E-6 6 5.702906759041223E-4 0.0 6.266930504440903E-6 7 5.765576064085631E-4 0.0 6.266930504440903E-6 8 5.765576064085631E-4 0.0 6.266930504440903E-6 9 5.828245369130041E-4 0.0 6.266930504440903E-6 10-11 6.078922589307677E-4 0.0 6.266930504440903E-6 12-13 6.329599809485313E-4 0.0 1.2533861008881807E-5 14-15 6.51760772461854E-4 0.0 1.2533861008881807E-5 16-17 6.548942377140744E-4 0.0 1.2533861008881807E-5 18-19 6.893623554884993E-4 0.0 1.2533861008881807E-5 20-21 7.457647300284675E-4 0.0 1.2533861008881807E-5 22-23 8.084340350728766E-4 6.266930504440903E-6 1.2533861008881807E-5 24-25 8.585694791084038E-4 6.266930504440903E-6 1.2533861008881807E-5 26-27 8.773702706217265E-4 9.400395756661354E-6 1.2533861008881807E-5 28-29 9.43173040918356E-4 1.2533861008881807E-5 1.2533861008881807E-5 30-31 0.001018376206971647 1.2533861008881807E-5 1.2533861008881807E-5 32-33 0.0010935793730249377 1.880079151332271E-5 1.2533861008881807E-5 34-35 0.001137447886556024 1.880079151332271E-5 1.2533861008881807E-5 36-37 0.0012533861008881806 1.880079151332271E-5 1.2533861008881807E-5 38-39 0.0013849916414814397 1.880079151332271E-5 1.2533861008881807E-5 40-41 0.00145706134228251 1.880079151332271E-5 1.2533861008881807E-5 42-43 0.0015792664871191078 2.1934256765543164E-5 1.2533861008881807E-5 44-45 0.0017046050972079258 2.5067722017763613E-5 1.2533861008881807E-5 46-47 0.0019302145953677984 2.5067722017763613E-5 1.2533861008881807E-5 48-49 0.0021746248850409936 2.5067722017763613E-5 1.2533861008881807E-5 50-51 0.0023877005221919845 2.5067722017763613E-5 1.2533861008881807E-5 52-53 0.002669712394891825 2.5067722017763613E-5 1.2533861008881807E-5 54-55 0.0028671207057817134 2.5067722017763613E-5 1.2533861008881807E-5 56-57 0.003180467231003759 2.5067722017763613E-5 1.566732626110226E-5 58-59 0.0034154771249202925 2.5067722017763613E-5 1.880079151332271E-5 60-61 0.0037319571153945578 2.8201187269984066E-5 1.880079151332271E-5 62-63 0.0041205068066698936 3.133465252220452E-5 1.880079151332271E-5 64-65 0.004546658080971875 3.133465252220452E-5 1.880079151332271E-5 66-67 0.004863138071446141 3.133465252220452E-5 1.880079151332271E-5 68-69 0.005229753505955934 3.133465252220452E-5 1.880079151332271E-5 70-71 0.005706040224293442 3.133465252220452E-5 1.880079151332271E-5 72-73 0.006201127734144274 3.133465252220452E-5 1.880079151332271E-5 74-75 0.006796486132066159 3.133465252220452E-5 1.880079151332271E-5 76-77 0.007582985910373494 3.133465252220452E-5 2.1934256765543164E-5 78-79 0.008410220736959692 3.133465252220452E-5 2.5067722017763613E-5 80-81 0.009450531200696883 3.133465252220452E-5 2.5067722017763613E-5 82-83 0.010500242060190734 3.133465252220452E-5 2.5067722017763613E-5 84-85 0.011618889155233434 3.133465252220452E-5 2.5067722017763613E-5 86-87 0.09467451913058873 3.133465252220452E-5 2.5067722017763613E-5 88-89 0.2264461333822154 3.133465252220452E-5 2.5067722017763613E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAATC 440755 0.0 75.63913 2 AATAAAT 451065 0.0 73.18253 1 TAAATCA 465040 0.0 72.27677 3 AATCATA 501520 0.0 68.15261 5 TCATAAA 506195 0.0 67.65261 7 AAATCAT 502445 0.0 66.937515 4 ATCATAA 522300 0.0 66.03006 6 CATAAAC 549425 0.0 62.206203 8 ATAAACA 615035 0.0 55.587967 9 ATGAATC 2615 0.0 47.556313 2 GTCCCCA 1765 0.0 42.882977 1 TGCCCGT 21380 0.0 42.250027 1 AGAAATC 4160 0.0 42.02514 2 AGAGCAC 2600 0.0 39.53142 94-95 GTAAATC 2335 0.0 39.172375 2 GATAAAT 4660 0.0 38.188408 1 AGTAAAT 2400 0.0 37.543873 1 GTCCCCC 925 0.0 36.52918 1 TTGCCCG 11990 0.0 36.22248 1 GAATCAT 3650 0.0 36.164757 4 >>END_MODULE