##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2212_lane2_GCCAAT_L002_R1_002.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15944342
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.229821274531115	31.0	28.0	34.0	16.0	34.0
2	30.125503619752763	31.0	30.0	34.0	25.0	34.0
3	30.831057937742777	31.0	30.0	34.0	27.0	34.0
4	33.64530519341541	35.0	33.0	37.0	28.0	37.0
5	34.31265338065233	35.0	35.0	37.0	30.0	37.0
6	35.116490521789814	37.0	35.0	37.0	32.0	37.0
7	35.334373510789106	37.0	35.0	37.0	33.0	37.0
8	35.43400982182082	37.0	35.0	37.0	33.0	37.0
9	37.100193011272836	39.0	37.0	39.0	33.0	39.0
10-11	37.134233403091734	39.0	37.0	39.0	33.0	39.0
12-13	37.161692080657275	39.0	37.0	39.0	33.5	39.0
14-15	38.421469559892586	40.0	38.0	41.0	34.0	41.0
16-17	38.33208866566697	40.0	38.0	41.0	33.0	41.0
18-19	38.346415987023526	40.0	38.0	41.0	33.0	41.0
20-21	38.32772669419164	40.0	38.0	41.0	33.5	41.0
22-23	38.33908483458773	40.0	38.0	41.0	34.0	41.0
24-25	38.31326177561117	40.0	38.0	41.0	34.0	41.0
26-27	38.22036591381424	40.0	38.0	41.0	34.0	41.0
28-29	37.95903499438704	40.0	37.0	41.0	33.0	41.0
30-31	38.11340802160312	40.0	37.5	41.0	33.0	41.0
32-33	37.90428874389315	40.0	37.0	41.0	33.0	41.0
34-35	37.84851899483242	40.0	37.0	41.0	33.0	41.0
36-37	37.83990824746664	40.0	37.0	41.0	33.0	41.0
38-39	37.86226860743517	40.0	37.0	41.0	33.0	41.0
40-41	37.85276985463567	40.0	37.0	41.0	33.0	41.0
42-43	37.87755917477527	40.0	37.0	41.0	33.0	41.0
44-45	37.82421726079991	40.0	37.0	41.0	33.0	41.0
46-47	37.805831269029426	40.0	37.0	41.0	33.0	41.0
48-49	37.77600656824595	40.0	37.0	41.0	33.0	41.0
50-51	37.74156576583348	40.0	37.0	41.0	33.0	41.0
52-53	37.674230091053445	40.0	37.0	41.0	32.0	41.0
54-55	37.58197576135446	40.0	36.5	41.0	32.0	41.0
56-57	37.47491973670036	39.0	36.0	41.0	32.0	41.0
58-59	37.35676049248582	39.0	36.0	41.0	31.0	41.0
60-61	37.189116732310175	39.0	36.0	40.0	31.0	41.0
62-63	36.95705252760871	39.0	35.0	40.0	31.0	41.0
64-65	36.622640527943275	38.0	35.0	40.0	31.0	41.0
66-67	36.273353002926186	37.0	35.0	39.5	31.0	41.0
68-69	35.89414107581078	37.0	34.5	39.0	31.0	40.5
70-71	35.48407686921325	36.0	34.0	39.0	30.5	40.0
72-73	35.068666643685106	36.0	34.0	37.5	30.0	39.0
74-75	34.69263130951574	35.0	34.0	37.0	30.0	39.0
76-77	32.03595748880289	33.0	30.5	34.5	27.0	36.5
78-79	33.89905663357837	35.0	33.0	36.0	29.5	37.0
80-81	33.99918328175127	35.0	34.0	36.0	30.0	37.0
82-83	33.86842902488183	35.0	34.0	35.0	30.0	36.5
84-85	33.73502385408111	35.0	33.0	35.0	30.0	36.0
86-87	33.632456036242	35.0	33.0	35.0	30.0	36.0
88-89	33.551648537080084	35.0	33.0	35.0	30.0	36.0
90-91	33.509669088046344	35.0	33.0	35.0	30.0	35.0
92-93	33.47103073509338	35.0	33.0	35.0	30.0	35.0
94-95	33.40913287712216	35.0	33.0	35.0	30.0	35.0
96-97	33.488887299216984	35.0	33.0	35.0	30.0	35.0
98-99	33.588506533816485	35.0	33.0	35.0	31.0	35.0
100-101	33.126688381640975	34.5	32.5	35.0	29.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1307	1	1.8660890081373616
1307	2	1.5083465686336197
1307	3	0.6348130762843311
1307	4	0.5666988060308924
1307	5	1.1324151656443675
1307	6	0.472639045998271
1307	7	0.2423415389972874
1307	8	0.1165489762183256
1307	9	0.024006863089290675
1307	10-11	0.094237849851325
1307	12-13	0.12172454830930235
1307	14-15	0.17461105210578864
1307	16-17	0.19484780737942486
1307	18-19	0.1922664319016647
1307	20-21	0.3184224639868063
1307	22-23	0.3314756660466571
1307	24-25	0.2760323374064555
1307	26-27	0.2834412384752696
1307	28-29	0.28648061114151346
1307	30-31	0.25421476977399493
1307	32-33	0.19826753172512923
1307	34-35	0.2694042816719673
1307	36-37	0.3168584492658013
1307	38-39	0.1626180906385386
1307	40-41	0.27029536147350086
1307	42-43	0.25515858835242256
1307	44-45	0.20775892544745744
1307	46-47	0.2366609580631689
1307	48-49	0.3285199190761787
1307	50-51	0.3354428271861636
1307	52-53	0.27214548579382836
1307	54-55	0.2830925087729881
1307	56-57	0.23996885181175287
1307	58-59	0.29275129882998385
1307	60-61	0.321005562620698
1307	62-63	0.21584684305057777
1307	64-65	0.26139989945888686
1307	66-67	0.16292336153293263
1307	68-69	0.20948872595491252
1307	70-71	0.2901526209690388
1307	72-73	0.3466666518660091
1307	74-75	0.17700777844428472
1307	76-77	0.1260078559231701
1307	78-79	0.1583716356025846
1307	80-81	0.1390444173507248
1307	82-83	0.20459480767059546
1307	84-85	0.23846848630698503
1307	86-87	0.2263542686219111
1307	88-89	0.23146704995694733
1307	90-91	0.19706200433087417
1307	92-93	0.1552585776839095
1307	94-95	0.1340260819000676
1307	96-97	0.12628242429165937
1307	98-99	0.17376793553221148
1307	100-101	0.1549150850483727
1306	1	0.3381292055658811
1306	2	0.9756091840175074
1306	3	0.4471511183540535
1306	4	-1.1871125505755131
1306	5	0.4442573956211291
1306	6	0.21868560170213414
1306	7	0.09259583851900999
1306	8	0.06212550934252903
1306	9	0.11910749423087452
1306	10-11	0.17111296302705625
1306	12-13	0.17213983057557414
1306	14-15	0.13154293020217267
1306	16-17	0.1726059956260002
1306	18-19	0.14027091293286986
1306	20-21	0.17055270581828808
1306	22-23	0.19999034223514656
1306	24-25	0.2058907722022596
1306	26-27	0.22552683493444192
1306	28-29	0.1892813027128568
1306	30-31	0.17425852958813692
1306	32-33	0.1661920849547016
1306	34-35	0.23746821297024212
1306	36-37	0.19168904029863398
1306	38-39	0.17661551931645647
1306	40-41	0.2087591837341165
1306	42-43	0.25527011052258075
1306	44-45	0.2343621909877882
1306	46-47	0.17028508072210258
1306	48-49	0.20447701257395323
1306	50-51	0.20596871135806794
1306	52-53	0.19852510761595
1306	54-55	0.19789756887310972
1306	56-57	0.13771485399853134
1306	58-59	0.19874744755446727
1306	60-61	0.23285883234674287
1306	62-63	0.15944534374737174
1306	64-65	0.15914466311885178
1306	66-67	0.13256770439407717
1306	68-69	0.1821884411027952
1306	70-71	0.1886401917209355
1306	72-73	0.09986346023263337
1306	74-75	0.08820031453831945
1306	76-77	-0.26100067001407723
1306	78-79	0.09745923400296164
1306	80-81	0.10249789332409165
1306	82-83	0.09663635096870848
1306	84-85	0.0912074188690184
1306	86-87	0.11957416803426923
1306	88-89	0.07262455136216772
1306	90-91	0.04824615676015753
1306	92-93	0.01490632120938784
1306	94-95	-0.003109777499311406
1306	96-97	0.07804648005672732
1306	98-99	0.055608685595096574
1306	100-101	0.005140755712119471
1305	1	0.033333061806974484
1305	2	0.9217046200360564
1305	3	0.4476119245888803
1305	4	-1.2506986704755505
1305	5	0.4723294915106777
1305	6	0.1723136303414421
1305	7	0.12611135196110013
1305	8	0.11858065842048404
1305	9	0.17781899785975241
1305	10-11	0.14349206022379235
1305	12-13	0.1485276045839754
1305	14-15	0.11104502055939491
1305	16-17	0.13244081128708984
1305	18-19	0.12121894990391269
1305	20-21	0.12388155529928468
1305	22-23	0.13355539150665408
1305	24-25	0.13178738828435854
1305	26-27	0.13003375876326828
1305	28-29	0.20296009558687444
1305	30-31	0.16673499844780793
1305	32-33	0.19667145491654736
1305	34-35	0.2067435564832678
1305	36-37	0.221181930130264
1305	38-39	0.19106235806370364
1305	40-41	0.22133281419731077
1305	42-43	0.21816540064939005
1305	44-45	0.2812638890128696
1305	46-47	0.23504780597465214
1305	48-49	0.19134801298795168
1305	50-51	0.1726597334273805
1305	52-53	0.20317150772653747
1305	54-55	0.15347861401508567
1305	56-57	0.18273579858878009
1305	58-59	0.19416952114058006
1305	60-61	0.17249492075097805
1305	62-63	0.18451935316243606
1305	64-65	0.20156603959760844
1305	66-67	0.19814658126156814
1305	68-69	0.16515265492961362
1305	70-71	0.18339046470471487
1305	72-73	0.09918759984098102
1305	74-75	0.07337218770918952
1305	76-77	-0.1344436748063984
1305	78-79	0.12634955451851937
1305	80-81	0.1257886908979131
1305	82-83	0.1138946028283172
1305	84-85	0.10962500398176189
1305	86-87	0.12189947336594997
1305	88-89	0.11013525030563187
1305	90-91	0.09985112741354385
1305	92-93	0.08427539014745378
1305	94-95	0.09316685750932407
1305	96-97	0.07410557430765152
1305	98-99	0.0952072743215453
1305	100-101	0.04318445492398837
1304	1	1.4048433385320571
1304	2	1.3856205148838114
1304	3	0.2978368904211166
1304	4	1.420264962290375
1304	5	1.2204402259864437
1304	6	0.43328527014491414
1304	7	0.22418080477989832
1304	8	0.11971296122324304
1304	9	0.1153701720590945
1304	10-11	0.07041107095562893
1304	12-13	0.05079400126587075
1304	14-15	0.029770561689751673
1304	16-17	0.10903596205452715
1304	18-19	0.08960618565524214
1304	20-21	0.002753151770022555
1304	22-23	0.0984970326978214
1304	24-25	0.026774560010920823
1304	26-27	0.0379733807888627
1304	28-29	0.05524736223900106
1304	30-31	0.07818502051576104
1304	32-33	-0.008009842330785943
1304	34-35	0.08190997443473691
1304	36-37	0.030153879591871657
1304	38-39	0.02390951616325765
1304	40-41	0.019350287687657897
1304	42-43	0.059786221668922224
1304	44-45	0.05190319512546182
1304	46-47	0.09280201217458739
1304	48-49	0.10364042142166596
1304	50-51	0.061885064244890486
1304	52-53	0.1494036931668603
1304	54-55	0.006754541923122304
1304	56-57	0.10452652136906693
1304	58-59	0.021616283015418958
1304	60-61	0.05553218243757385
1304	62-63	0.07632123499305266
1304	64-65	0.05484272714257088
1304	66-67	0.07530485260721065
1304	68-69	0.1091429092615428
1304	70-71	0.08373767800512155
1304	72-73	0.10535451014544606
1304	74-75	0.056012442367446624
1304	76-77	-0.24236908318635386
1304	78-79	0.01781538307547237
1304	80-81	0.010050121094614894
1304	82-83	0.06380740323012901
1304	84-85	-0.004182404967458808
1304	86-87	0.04620237095598867
1304	88-89	0.11592719921909378
1304	90-91	0.12006107415803058
1304	92-93	0.13182860691645715
1304	94-95	0.06658464651928142
1304	96-97	0.0747398754392492
1304	98-99	0.10136929508157522
1304	100-101	-0.0021736868889448147
1216	1	0.9433584074378203
1216	2	0.5954388171436449
1216	3	0.3868318261783692
1216	4	0.9395011428009639
1216	5	0.3637432071055855
1216	6	0.20783806220545387
1216	7	0.01738311812650295
1216	8	0.06131552278119301
1216	9	0.02916699755513008
1216	10-11	0.05659159578508621
1216	12-13	0.02568257971799426
1216	14-15	0.022552426230497247
1216	16-17	0.05796468312420444
1216	18-19	5.476650219193857E-4
1216	20-21	-0.005073565997889773
1216	22-23	-0.004115853002325309
1216	24-25	-0.04163748135082557
1216	26-27	0.022188076702910564
1216	28-29	0.06405111581244682
1216	30-31	0.005447940907302495
1216	32-33	-0.0382826817362556
1216	34-35	-0.08741694808963985
1216	36-37	-0.006117659985889645
1216	38-39	-0.056329602159429726
1216	40-41	-0.0761428188846267
1216	42-43	-0.09865559106692956
1216	44-45	-0.09207651206004641
1216	46-47	-0.08637257529700548
1216	48-49	-0.10685700764931738
1216	50-51	-0.013186123310745756
1216	52-53	-0.09218945921703892
1216	54-55	-0.12556370182404208
1216	56-57	-0.12951170874623585
1216	58-59	-0.10011216761431996
1216	60-61	-0.12011732621670745
1216	62-63	-4.4144028105108646E-4
1216	64-65	-0.08270182127913017
1216	66-67	-0.09511135422067696
1216	68-69	-0.10575582710588805
1216	70-71	-0.03990745116004746
1216	72-73	-0.07895837959891594
1216	74-75	-0.12177373527722324
1216	76-77	-0.2232996190764247
1216	78-79	-0.06370880376397992
1216	80-81	-0.06151487926536703
1216	82-83	-0.11672247552686343
1216	84-85	-0.04523015000144426
1216	86-87	-0.07769848745419239
1216	88-89	-0.023318135778907845
1216	90-91	0.013635405251527288
1216	92-93	-0.019249220090920005
1216	94-95	0.008662811808456183
1216	96-97	0.005331342870412925
1216	98-99	-0.011115251857127362
1216	100-101	-0.055912135910524796
1308	1	-0.5756519905087494
1308	2	-0.46550631637966333
1308	3	-0.2845352914904886
1308	4	-0.17369624121958083
1308	5	0.5362202186379363
1308	6	0.1914345490187941
1308	7	0.05607668632514162
1308	8	-0.014876898868678268
1308	9	-0.08153431381964538
1308	10-11	-0.021088747986929945
1308	12-13	-0.0012429815759702478
1308	14-15	-0.032573703024645795
1308	16-17	0.023758621241036337
1308	18-19	-0.03731778266078578
1308	20-21	-0.08924869524867773
1308	22-23	-0.08955483323591551
1308	24-25	-0.03973690277984332
1308	26-27	-0.014526924642609629
1308	28-29	-0.07329025548445145
1308	30-31	-0.0547667872493065
1308	32-33	-0.0432218882604829
1308	34-35	-0.008901915954311335
1308	36-37	-0.08019502766027387
1308	38-39	-0.05218754682722704
1308	40-41	-0.057969185032064274
1308	42-43	-0.12241880070202882
1308	44-45	-0.08247914410225832
1308	46-47	-0.09386925905586452
1308	48-49	-0.04762143100334271
1308	50-51	-0.09229630940532019
1308	52-53	-0.06115401099985718
1308	54-55	-0.08068383012076197
1308	56-57	-0.004816392231205668
1308	58-59	-0.01060148070311584
1308	60-61	-0.0430442973248546
1308	62-63	-0.08976179863400802
1308	64-65	-0.1999134015141948
1308	66-67	-0.08658454106436864
1308	68-69	-0.07392664781134783
1308	70-71	-0.09347125045557902
1308	72-73	-0.08508927144118417
1308	74-75	-0.07456957956920718
1308	76-77	-0.27731659754530824
1308	78-79	-0.07750608768334644
1308	80-81	-0.09316931661722805
1308	82-83	-0.09095164118542698
1308	84-85	-0.036934883129490004
1308	86-87	-0.06690166588573021
1308	88-89	-0.04381428218927397
1308	90-91	-0.06375155779704045
1308	92-93	-0.07973082559433209
1308	94-95	-0.022001929669684728
1308	96-97	-0.029578733965827553
1308	98-99	-0.00814988601248956
1308	100-101	-0.053370104319675704
1303	1	-2.491805904780861
1303	2	-0.2862135498230991
1303	3	-0.6369716100683966
1303	4	-0.16025538523867056
1303	5	-0.6899065486737683
1303	6	-0.27865650008717324
1303	7	-0.11205037940285933
1303	8	-0.10690300873570635
1303	9	-0.0779763172393686
1303	10-11	-0.1214057486901865
1303	12-13	-0.13647124881720885
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1211	20-21	0.5378101096257595
1211	22-23	0.4751756174868049
1211	24-25	0.5191450185962125
1211	26-27	0.5277981420166569
1211	28-29	0.5425686995688466
1211	30-31	0.5420333274515627
1211	32-33	0.5897460326194093
1211	34-35	0.5957872592365447
1211	36-37	0.6341910135454469
1211	38-39	0.6284223235236652
1211	40-41	0.5997407508236066
1211	42-43	0.5629441533344774
1211	44-45	0.579088092568945
1211	46-47	0.5456109769069357
1211	48-49	0.5598637962019026
1211	50-51	0.5302443051120207
1211	52-53	0.5695392054636699
1211	54-55	0.5840237912863628
1211	56-57	0.5677371402813662
1211	58-59	0.5566717596338506
1211	60-61	0.4839048942444748
1211	62-63	0.49126893228099533
1211	64-65	0.5376465666813459
1211	66-67	0.533555553698946
1211	68-69	0.4672012324340358
1211	70-71	0.44606388535348884
1211	72-73	0.37994807361533844
1211	74-75	0.42491718954004654
1211	76-77	0.3913030397603876
1211	78-79	0.39030884977724156
1211	80-81	0.3865435435466864
1211	82-83	0.3280611211201858
1211	84-85	0.3739127039617216
1211	86-87	0.36171317100946965
1211	88-89	0.33903605559678596
1211	90-91	0.3234867654017748
1211	92-93	0.2973796519276135
1211	94-95	0.27141441379445297
1211	96-97	0.26087787128943773
1211	98-99	0.236184894544472
1211	100-101	0.22990586594895035
1208	1	2.2220009257800086
1208	2	1.7859928345535252
1208	3	1.3819497552191145
1208	4	1.324350185849518
1208	5	1.4483859469927935
1208	6	0.7452773544663529
1208	7	0.41796825993787934
1208	8	0.2820160444594535
1208	9	0.239054570062045
1208	10-11	0.17741208130271957
1208	12-13	0.2825429317872761
1208	14-15	0.3271407183902184
1208	16-17	0.3832646195762095
1208	18-19	0.38122191166007724
1208	20-21	0.4219625645649572
1208	22-23	0.37767355740596287
1208	24-25	0.35053292078131193
1208	26-27	0.399269772035737
1208	28-29	0.4164910501324641
1208	30-31	0.41767992250801456
1208	32-33	0.5218187299375572
1208	34-35	0.4680988798333985
1208	36-37	0.532911388618686
1208	38-39	0.46080227687686204
1208	40-41	0.4867223833073311
1208	42-43	0.4989495385964915
1208	44-45	0.4011691010845695
1208	46-47	0.4786436897757156
1208	48-49	0.4220766697427436
1208	50-51	0.41693704932472286
1208	52-53	0.3929642900127277
1208	54-55	0.445186853736665
1208	56-57	0.49312274621153307
1208	58-59	0.42946381393164756
1208	60-61	0.44118118203799384
1208	62-63	0.4634640503308418
1208	64-65	0.41335636656363306
1208	66-67	0.40255832694313654
1208	68-69	0.43756510573956575
1208	70-71	0.4199688150125027
1208	72-73	0.36472422989062636
1208	74-75	0.3981803190718054
1208	76-77	0.39215713054969115
1208	78-79	0.3211276551021882
1208	80-81	0.3505161267332966
1208	82-83	0.33329748586035635
1208	84-85	0.2797574997593486
1208	86-87	0.3050730527671064
1208	88-89	0.2964970399562148
1208	90-91	0.31265883295671415
1208	92-93	0.3116040210486659
1208	94-95	0.27388442746889297
1208	96-97	0.21433053891642118
1208	98-99	0.24063567318849977
1208	100-101	0.2229048015306887
1209	1	1.3715221788242324
1209	2	1.4680091566009352
1209	3	1.058320651041793
1209	4	0.84929722314007
1209	5	1.2504810970642666
1209	6	0.6041208684175743
1209	7	0.3457240036490248
1209	8	0.24436140788829874
1209	9	0.17789902001901936
1209	10-11	0.245877242351753
1209	12-13	0.2287217559502892
1209	14-15	0.23866481770026837
1209	16-17	0.21860697210878044
1209	18-19	0.2723344297838466
1209	20-21	0.34150398258567094
1209	22-23	0.3882565626239227
1209	24-25	0.41297671466671915
1209	26-27	0.318492857781699
1209	28-29	0.438577462752221
1209	30-31	0.36904786372613785
1209	32-33	0.42644448241281196
1209	34-35	0.4669859020936542
1209	36-37	0.49528485442886705
1209	38-39	0.48451262613947677
1209	40-41	0.4196651944847787
1209	42-43	0.364763742572876
1209	44-45	0.402812814531714
1209	46-47	0.45857624347099346
1209	48-49	0.3746245290231869
1209	50-51	0.33772259808327476
1209	52-53	0.388957007227944
1209	54-55	0.3849234259020662
1209	56-57	0.4563807945703573
1209	58-59	0.4659152048017319
1209	60-61	0.3365975648501376
1209	62-63	0.3952830944541077
1209	64-65	0.3473506240481896
1209	66-67	0.3476632005507412
1209	68-69	0.3648115802770633
1209	70-71	0.36905686124197246
1209	72-73	0.28765963209581713
1209	74-75	0.34421240689408705
1209	76-77	0.29905762988776985
1209	78-79	0.2772191296767659
1209	80-81	0.2908299381186694
1209	82-83	0.264839605242031
1209	84-85	0.26206395853050424
1209	86-87	0.24558072327551628
1209	88-89	0.20047719379312667
1209	90-91	0.25147493783172337
1209	92-93	0.212277348666845
1209	94-95	0.2371058424896546
1209	96-97	0.22657758596092492
1209	98-99	0.2007034453361385
1209	100-101	0.18379498278743966
1214	1	0.9841956476768097
1214	2	1.189601599570782
1214	3	0.50061802637547
1214	4	-1.5093052413406127
1214	5	0.3404076705376866
1214	6	0.1727658446410345
1214	7	0.13054487279478622
1214	8	0.17407510553476868
1214	9	0.20831315351419732
1214	10-11	0.19417677125861132
1214	12-13	0.1624376917646444
1214	14-15	0.23924043470387346
1214	16-17	0.26644359131226736
1214	18-19	0.2654411467596063
1214	20-21	0.33922846160231046
1214	22-23	0.24387314060167142
1214	24-25	0.23758998690775712
1214	26-27	0.23175135353766052
1214	28-29	0.1855844948862142
1214	30-31	0.31744705396678796
1214	32-33	0.3962847352815544
1214	34-35	0.3041005705605926
1214	36-37	0.3587335260368576
1214	38-39	0.3997251528005279
1214	40-41	0.39038668424581147
1214	42-43	0.35250596153836256
1214	44-45	0.32679073549678606
1214	46-47	0.3722022003830361
1214	48-49	0.31853062050763725
1214	50-51	0.33053052326702925
1214	52-53	0.3272648332716983
1214	54-55	0.30299366053947807
1214	56-57	0.2868572695491025
1214	58-59	0.3650486805912152
1214	60-61	0.2904900117113911
1214	62-63	0.3500381907751873
1214	64-65	0.3634167679720193
1214	66-67	0.3049108594022698
1214	68-69	0.267147876120319
1214	70-71	0.2882680264127728
1214	72-73	0.3037425054242462
1214	74-75	0.2590177202318529
1214	76-77	0.402035729443071
1214	78-79	0.2191351927834546
1214	80-81	0.21082836403023464
1214	82-83	0.22222149446551498
1214	84-85	0.1514866073663086
1214	86-87	0.17310304471799753
1214	88-89	0.19829991188722573
1214	90-91	0.1509586598529964
1214	92-93	0.17061256548856818
1214	94-95	0.17776020968495487
1214	96-97	0.2030737541547154
1214	98-99	0.1604121960406033
1214	100-101	0.11252193012128231
1215	1	0.8896811839147176
1215	2	1.2097236506105844
1215	3	0.9423873261604889
1215	4	0.389741781833969
1215	5	1.0489287320579095
1215	6	0.4739083794831913
1215	7	0.29131147434910787
1215	8	0.18802798712503233
1215	9	0.14239388766721817
1215	10-11	0.20761250360367
1215	12-13	0.1857129250702627
1215	14-15	0.1235171107002131
1215	16-17	0.1728607974893137
1215	18-19	0.2084571806083062
1215	20-21	0.22811878417590492
1215	22-23	0.23622849485649766
1215	24-25	0.18876392797380248
1215	26-27	0.24907608055466568
1215	28-29	0.23089940185198543
1215	30-31	0.22857178904948938
1215	32-33	0.2981712764396178
1215	34-35	0.2534997866234292
1215	36-37	0.3511952029543366
1215	38-39	0.29604389438534895
1215	40-41	0.28924694023667996
1215	42-43	0.27414461269393087
1215	44-45	0.2611517644370309
1215	46-47	0.24527928351921702
1215	48-49	0.3093112035851817
1215	50-51	0.26220786117056605
1215	52-53	0.14719714578175314
1215	54-55	0.2510891328363414
1215	56-57	0.23730131703830182
1215	58-59	0.19574494289472
1215	60-61	0.2593744892487564
1215	62-63	0.20459863891546348
1215	64-65	0.24063568077997388
1215	66-67	0.2939608991833822
1215	68-69	0.2486762204634516
1215	70-71	0.13122513327097352
1215	72-73	0.22273869494618026
1215	74-75	0.23591487850439563
1215	76-77	0.33397671603879076
1215	78-79	0.2341429780245292
1215	80-81	0.21092100790045265
1215	82-83	0.24814713827917956
1215	84-85	0.2033558195317724
1215	86-87	0.22708095681719698
1215	88-89	0.19963110106138515
1215	90-91	0.18844337292377134
1215	92-93	0.1525029754116929
1215	94-95	0.16971455492251408
1215	96-97	0.1708505029651235
1215	98-99	0.12386709809657503
1215	100-101	0.028043271318807683
1212	1	1.4273506505379245
1212	2	1.517551679964967
1212	3	1.0549739723726006
1212	4	2.11713922780136
1212	5	1.5171662040184657
1212	6	0.6482859907104768
1212	7	0.315976018257075
1212	8	0.20981897242045733
1212	9	0.1915128992812427
1212	10-11	0.08835036747970548
1212	12-13	0.17220007202421783
1212	14-15	0.1843938794284199
1212	16-17	0.2404027152817605
1212	18-19	0.23333483334804583
1212	20-21	0.20710858215630168
1212	22-23	0.207703818531634
1212	24-25	0.20733737076327685
1212	26-27	0.24820368771926127
1212	28-29	0.30219072302912764
1212	30-31	0.22926387342928223
1212	32-33	0.2206547228035518
1212	34-35	0.2657768921243502
1212	36-37	0.2648368045913472
1212	38-39	0.27985813612244215
1212	40-41	0.29255578935172366
1212	42-43	0.23562692179556421
1212	44-45	0.21808565945342195
1212	46-47	0.2075137208132034
1212	48-49	0.24535621471171254
1212	50-51	0.21758667512285257
1212	52-53	0.28375506294784003
1212	54-55	0.28363365541770946
1212	56-57	0.31538999915192534
1212	58-59	0.26000322305186785
1212	60-61	0.2986109099002334
1212	62-63	0.29534427991890055
1212	64-65	0.2724094212795194
1212	66-67	0.3009534342122251
1212	68-69	0.2424143513189847
1212	70-71	0.26219930937839564
1212	72-73	0.272019066058256
1212	74-75	0.2614613875908418
1212	76-77	0.4389138080506285
1212	78-79	0.2517108500912286
1212	80-81	0.26640144163329893
1212	82-83	0.2019923909031789
1212	84-85	0.22296881663362456
1212	86-87	0.24911647988638919
1212	88-89	0.21710673060417207
1212	90-91	0.17845640788748085
1212	92-93	0.20513213720445833
1212	94-95	0.21303615307196822
1212	96-97	0.23359748850620576
1212	98-99	0.17903046774092246
1212	100-101	0.1672407067428736
1213	1	1.2713935715301545
1213	2	1.0401268292496582
1213	3	0.7425246640479841
1213	4	1.4413144072661055
1213	5	1.3060391621184237
1213	6	0.5349944681026599
1213	7	0.2229169346418658
1213	8	0.21892677679798567
1213	9	0.15765571246747356
1213	10-11	0.09218816544498054
1213	12-13	0.12564371363463067
1213	14-15	0.09964688014559187
1213	16-17	0.19830870380695131
1213	18-19	0.17725065626905234
1213	20-21	0.10966047320471972
1213	22-23	0.16180845885943285
1213	24-25	0.13154124862761307
1213	26-27	0.1324070663438377
1213	28-29	0.15650855476838643
1213	30-31	0.18982200385887182
1213	32-33	0.23677998788508603
1213	34-35	0.23835779640135257
1213	36-37	0.25965323201642576
1213	38-39	0.2119487758610603
1213	40-41	0.22287658745210592
1213	42-43	0.19039642151647485
1213	44-45	0.19677296774589337
1213	46-47	0.18668226623967143
1213	48-49	0.17920693975934654
1213	50-51	0.22009301176409224
1213	52-53	0.1427365567662804
1213	54-55	0.2340217105098219
1213	56-57	0.20466959913014193
1213	58-59	0.19651650462427028
1213	60-61	0.16219078703490197
1213	62-63	0.17507807954331156
1213	64-65	0.1649850940115698
1213	66-67	0.1585388622361208
1213	68-69	0.11337266913812982
1213	70-71	0.12038509988354917
1213	72-73	0.16533608680732215
1213	74-75	0.1930917356560471
1213	76-77	0.48302191715054477
1213	78-79	0.20777966469674425
1213	80-81	0.16277120573315784
1213	82-83	0.16534530480991805
1213	84-85	0.11016062453506947
1213	86-87	0.10926055050510541
1213	88-89	0.15274815624585614
1213	90-91	0.1757115534876874
1213	92-93	0.15893163397658583
1213	94-95	0.13981958805833727
1213	96-97	0.13561220403043706
1213	98-99	0.0964883302943278
1213	100-101	0.13067909475331874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	85.0
15	3373.0
16	2273.0
17	2538.0
18	1531.0
19	1356.0
20	1644.0
21	1526.0
22	2084.0
23	3195.0
24	6913.0
25	17255.0
26	42542.0
27	94250.0
28	177388.0
29	288163.0
30	421718.0
31	566710.0
32	733315.0
33	937331.0
34	1223531.0
35	1660607.0
36	2358830.0
37	3667281.0
38	3474104.0
39	253817.0
40	982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.09290844363474	41.80503027343493	13.429917647275754	36.67214363565458
2	8.445484424578414	45.33792013760664	20.760772822689034	25.45582261512591
3	5.754423610250015	18.088041756113544	43.86493049564468	32.292604137991766
4	8.756842635872285	39.555139908804165	12.419523677019306	39.26849377830424
5	9.559154527457151	42.51402898350657	15.420260845284298	32.50655564375198
6	8.250070948175136	46.98885401344507	18.426116559850072	26.334958478529717
7	6.209098080244922	30.07262894797151	38.71483162245074	25.003441349332828
8	6.421260514298995	32.9267358661442	16.374709005989192	44.27729461356761
9	6.042393666271762	56.410092039021166	17.930534400942697	19.616979893764373
10-11	6.019035278702294	45.34071540039123	28.535344544083134	20.104904776823336
12-13	5.153397252597582	57.225700516140535	16.535565660867714	21.08533657039417
14-15	5.806271966559395	45.7744596691653	16.107720811282686	32.31154755299262
16-17	5.673792442881278	55.569512101332606	16.64131784866762	22.115377607118496
18-19	18.01448083919193	30.954939278953418	17.891221468499147	33.1393584133555
20-21	28.165431196518213	31.945376342247194	17.837735272060787	22.0514571891738
22-23	3.717818799462516	45.359658745787115	17.417205705005788	33.50531674974459
24-25	3.5772364427427874	44.74652968589378	16.941318399444675	34.734915471918754
26-27	3.560315409324894	44.95740947802115	29.08980294592468	22.39247216672927
28-29	3.5739252398939345	43.758111578043774	16.8862418129342	35.781721369128086
30-31	3.5683335354484416	33.216280671125986	29.12814569533279	34.08724009809278
32-33	3.633232992402818	45.561322239198	21.337946115681795	29.46749865271738
34-35	3.619577550847845	45.03396955818467	20.98344456714719	30.363008323820296
36-37	3.620981810157834	44.79864414465916	21.191036417795345	30.389337627387665
38-39	3.627055362405652	45.00351773339945	21.4248343005586	29.9445926036363
40-41	3.6325893632531256	44.86381389534745	21.237458539492486	30.26613820190694
42-43	3.612849518097705	44.53893536582481	21.399679649928597	30.448535466148886
44-45	3.6138382716672557	44.85457483008242	21.412678419309902	30.11890847894042
46-47	3.640067801987644	44.81103280523379	21.157549531976315	30.391349860802247
48-49	3.599257363537045	44.60043097495571	21.367769619436483	30.432542042070764
50-51	3.6020907941572387	44.760519159778724	21.51847802660092	30.118912019463117
52-53	3.6008735626967514	44.72738368922192	21.323044582884894	30.348698165196435
54-55	3.579820858061157	44.45720331384691	21.517191829209885	30.44578399888205
56-57	3.5818625915211446	44.75084666012496	21.51466036438779	30.152630383966105
58-59	3.5931759770588907	44.704676642704236	21.313350084955417	30.38879729528146
60-61	3.5590388039432637	44.50208703353118	21.505713057595496	30.433161104930058
62-63	3.546163392010059	44.72468789136109	21.61193266570084	30.117216050928008
64-65	3.5558693211233705	44.65304475865825	21.414130208228276	30.376955711990107
66-67	3.524778789143774	44.46398488334171	21.556541131664673	30.45469519584984
68-69	3.5139047813379793	44.70357076578981	21.619750217495305	30.162774235376904
70-71	3.5237046146829343	44.69780450989016	21.391256454529163	30.38723442089774
72-73	3.490012591087907	44.438767067597595	21.58609232965089	30.48512801166361
74-75	3.4766461483783555	44.67338151907263	21.689045747461847	30.16092658508717
76-77	3.4723714152541136	44.71764876550575	21.471315594980474	30.33866422425966
78-79	3.4370701276732545	44.48437299387173	21.632199484632913	30.446357393822105
80-81	3.4247368112005696	44.75669614259566	21.683320340174348	30.135246706029424
82-83	3.4291268538480364	44.76347643233033	21.468376444336336	30.339020269485296
84-85	3.391076038371178	44.5337063321105	21.634453563138678	30.440764066379643
86-87	3.398116351136746	44.77625518821349	21.74997301275101	30.075655447898754
88-89	3.4286800405367885	44.79470323970223	21.5290953061237	30.247521413637283
90-91	3.437931167965029	44.57638845599595	21.664909253157447	30.320771122881577
92-93	3.5262121158906985	44.776854757880386	21.685417193561033	30.011515932667887
94-95	3.579542961337051	44.79922628483305	21.47129470686269	30.149936046967213
96-97	3.6451465389021775	44.64538160858742	21.597641053586038	30.11183079892436
98-99	3.7132065793460485	44.88711234994846	21.62501668173855	29.774664388966944
100-101	3.8285606876737335	44.91495508786348	21.287340864966783	29.969143359495998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3978.0
1	3937.0
2	3397.0
3	2646.0
4	2418.0
5	2478.5
6	2617.0
7	2673.0
8	2605.5
9	2721.0
10	2955.5
11	3140.5
12	3348.0
13	3977.5
14	5032.0
15	5971.5
16	6975.5
17	8564.0
18	11517.0
19	15686.5
20	21999.5
21	33944.0
22	55422.5
23	92435.5
24	151829.5
25	238546.5
26	359050.0
27	517940.0
28	709412.0
29	914732.0
30	1109778.5
31	1264826.0
32	1361203.5
33	1384852.5
34	1328916.5
35	1221066.0
36	1084026.5
37	934209.5
38	784433.5
39	641364.0
40	517382.5
41	416603.5
42	335976.5
43	273284.5
44	228200.0
45	195047.5
46	167807.0
47	146877.0
48	128554.0
49	110315.5
50	96937.5
51	87084.0
52	79126.5
53	74676.5
54	71621.0
55	68177.5
56	65534.0
57	63426.0
58	61075.0
59	58741.0
60	56328.5
61	53103.0
62	49355.0
63	45383.5
64	41298.5
65	37362.0
66	33124.5
67	29113.0
68	25551.5
69	21969.0
70	18624.0
71	15799.0
72	13391.0
73	11428.0
74	9824.5
75	8279.5
76	7123.0
77	6459.5
78	5940.5
79	5390.5
80	4910.5
81	4581.5
82	4328.0
83	3954.5
84	3608.5
85	3498.0
86	3402.5
87	3352.0
88	2857.0
89	2365.5
90	2184.5
91	2011.5
92	1815.0
93	1542.0
94	1389.0
95	1288.0
96	1241.5
97	1220.0
98	1214.5
99	1216.0
100	1216.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03483999099114977
3	0.0
4	0.0
5	5.019873049920504E-5
6	4.1420257970387276E-4
7	3.138484498648443E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.294451217798492E-5
26-27	0.0
28-29	0.0
30-31	0.002994442713161351
32-33	0.0
34-35	0.0026271499109128365
36-37	0.0
38-39	0.0
40-41	3.22838280523286E-6
42-43	0.0
44-45	0.0
46-47	2.7744814942900353E-4
48-49	0.0
50-51	1.5120076249887135E-4
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.1722345885824475E-4
90-91	0.0
92-93	0.002982581722739203
94-95	0.017513646049213346
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	7684.0
5-9	11322.0
10-14	7774.0
15-19	10583.0
20-24	24216.0
25-29	77587.0
30-34	140814.0
35-39	162114.0
40-44	142877.0
45-49	134909.0
50-54	126926.0
55-59	119940.0
60-64	115780.0
65-69	116108.0
70-74	136714.0
75-79	130001.0
80-84	129944.0
85-89	114846.0
90-94	155219.0
95-99	301316.0
100-102	1.3777668E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.68202176009822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.7229633269939	84.94946858272453
2	3.963426873385193	7.108962702069781
3	0.7001494881864878	1.8837246490458672
4	0.19827092453559475	0.71125349474384
5	0.1308220939131733	0.5866194936511412
6	0.055682958246365696	0.2996256163870325
7	0.04774023659974981	0.29970086562997006
8	0.02785546474677586	0.19985075164464014
9	0.02012288728223797	0.16241950936095026
>10	0.12101074763813309	2.4228613955852563
>50	0.009399521048596603	0.5580810908767538
>100	0.00220097376886366	0.3552084286409977
>500	2.110241130714302E-4	0.11619843338908376
>1k	1.2909028599232452E-4	0.2257162655436204
>5k	7.394057538248702E-6	0.03820199921303118
>10k+	6.995198367307561E-6	0.08210672149343035
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.202117591306057E-4	0.0	0.0
2	4.202117591306057E-4	0.0	0.0
3	4.264835764310625E-4	0.0	6.271817300456802E-6
4	4.264835764310625E-4	0.0	6.271817300456802E-6
5	4.327553937315193E-4	0.0	6.271817300456802E-6
6	4.390272110319761E-4	0.0	6.271817300456802E-6
7	4.452990283324329E-4	6.271817300456802E-6	6.271817300456802E-6
8	4.452990283324329E-4	6.271817300456802E-6	1.2543634600913604E-5
9	4.5784266293334654E-4	6.271817300456802E-6	1.2543634600913604E-5
10-11	4.641144802338033E-4	6.271817300456802E-6	1.2543634600913604E-5
12-13	4.954735667360873E-4	6.271817300456802E-6	1.2543634600913604E-5
14-15	5.236967445881429E-4	6.271817300456802E-6	1.2543634600913604E-5
16-17	5.425121964895133E-4	6.271817300456802E-6	1.2543634600913604E-5
18-19	5.70735374341569E-4	6.271817300456802E-6	1.2543634600913604E-5
20-21	6.209099127452234E-4	6.271817300456802E-6	1.2543634600913604E-5
22-23	6.616767251981926E-4	6.271817300456802E-6	1.2543634600913604E-5
24-25	6.898999030502482E-4	6.271817300456802E-6	1.2543634600913604E-5
26-27	7.149871722520754E-4	1.2543634600913604E-5	1.2543634600913604E-5
28-29	7.871130712073285E-4	1.2543634600913604E-5	1.2543634600913604E-5
30-31	8.937339653150942E-4	1.2543634600913604E-5	1.2543634600913604E-5
32-33	9.909471334721746E-4	1.2543634600913604E-5	1.2543634600913604E-5
34-35	0.0010411216718758292	1.2543634600913604E-5	1.2543634600913604E-5
36-37	0.001110111662180854	1.5679543251142005E-5	1.2543634600913604E-5
38-39	0.0012324120995397614	1.8815451901370405E-5	1.2543634600913604E-5
40-41	0.001370392080149811	1.8815451901370405E-5	1.2543634600913604E-5
42-43	0.001502100243459404	1.8815451901370405E-5	1.5679543251142005E-5
44-45	0.0016526238586703672	1.8815451901370405E-5	1.8815451901370405E-5
46-47	0.0018250988344329293	1.8815451901370405E-5	1.8815451901370405E-5
48-49	0.002016389262096862	1.8815451901370405E-5	1.8815451901370405E-5
50-51	0.00217632060325851	1.8815451901370405E-5	1.8815451901370405E-5
52-53	0.002364475122272214	1.8815451901370405E-5	1.8815451901370405E-5
54-55	0.0026749300786448257	1.8815451901370405E-5	1.8815451901370405E-5
56-57	0.0029634336744658388	1.8815451901370405E-5	1.8815451901370405E-5
58-59	0.0031860831886320554	1.8815451901370405E-5	1.8815451901370405E-5
60-61	0.003499674053654895	2.1951360551598804E-5	1.8815451901370405E-5
62-63	0.003850895822480476	2.5087269201827207E-5	1.8815451901370405E-5
64-65	0.004311874394064051	2.5087269201827207E-5	1.8815451901370405E-5
66-67	0.004691319340741688	2.5087269201827207E-5	1.8815451901370405E-5
68-69	0.005045677018217497	2.5087269201827207E-5	1.8815451901370405E-5
70-71	0.00541257833029422	2.5087269201827207E-5	1.8815451901370405E-5
72-73	0.005942546892182819	2.5087269201827207E-5	1.8815451901370405E-5
74-75	0.006519554083824845	2.5087269201827207E-5	1.8815451901370405E-5
76-77	0.007265900342579205	2.5087269201827207E-5	1.8815451901370405E-5
78-79	0.008015382509983793	2.5087269201827207E-5	1.8815451901370405E-5
80-81	0.008912252383949115	2.5087269201827207E-5	1.8815451901370405E-5
82-83	0.009906335426071517	2.5087269201827207E-5	1.8815451901370405E-5
84-85	0.010985088001750087	2.5087269201827207E-5	1.8815451901370405E-5
86-87	0.09512151708737808	2.5087269201827207E-5	1.8815451901370405E-5
88-89	0.2290593114472833	2.5087269201827207E-5	1.8815451901370405E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAATC	444960	0.0	75.59035	2
AATAAAT	453345	0.0	73.20849	1
TAAATCA	469320	0.0	72.26285	3
AATCATA	506880	0.0	68.43793	5
TCATAAA	511775	0.0	67.92159	7
AAATCAT	507010	0.0	67.07968	4
ATCATAA	527545	0.0	66.37981	6
CATAAAC	555090	0.0	62.512268	8
ATGAATC	2855	0.0	59.010582	2
ATAAACA	621160	0.0	55.886497	9
AGAAATC	4910	0.0	46.88175	2
GATAAAT	5570	0.0	43.9883	1
GAATCAT	4005	0.0	43.74719	4
GTAAATC	2340	0.0	43.699295	2
GTCCCCA	1790	0.0	43.52979	1
TGCCCGT	20205	0.0	40.949047	1
AGTAAAT	2350	0.0	40.439716	1
TGAATCA	4260	0.0	40.065693	3
AATGAAT	4230	0.0	39.822155	1
AGAGCAC	2570	0.0	39.562515	94-95
>>END_MODULE