##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2212_lane2_GCCAAT_L002_R1_003.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15952602
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.622372889388203	31.0	30.0	34.0	16.0	34.0
2	30.32269668863113	31.0	30.0	34.0	25.0	34.0
3	31.239939298628094	31.0	31.0	34.0	28.0	34.0
4	34.07223363854798	37.0	35.0	37.0	28.0	37.0
5	34.247606032535245	37.0	35.0	37.0	28.0	37.0
6	35.19596710102167	37.0	35.0	37.0	32.0	37.0
7	35.433468460700404	37.0	35.0	37.0	33.0	37.0
8	35.51911831497049	37.0	35.0	37.0	33.0	37.0
9	37.227749457863986	39.0	37.0	39.0	34.0	39.0
10-11	36.90068715263014	39.0	37.0	39.0	32.5	39.0
12-13	37.2566762055904	39.0	37.0	39.0	34.0	39.0
14-15	38.50010846201647	40.0	38.0	41.0	34.0	41.0
16-17	38.47207355970598	40.0	38.0	41.0	34.0	41.0
18-19	38.53923980771984	40.0	38.0	41.0	34.0	41.0
20-21	38.56207483654068	40.0	38.0	41.0	34.0	41.0
22-23	38.58029423706787	40.0	38.0	41.0	34.0	41.0
24-25	38.56299280369964	40.0	38.0	41.0	34.0	41.0
26-27	38.44766893132255	40.0	38.0	41.0	34.0	41.0
28-29	38.19751764576307	40.0	38.0	41.0	33.5	41.0
30-31	38.37971159590923	40.0	38.0	41.0	34.0	41.0
32-33	38.17156256709637	40.0	38.0	41.0	33.0	41.0
34-35	38.15451182659811	40.0	38.0	41.0	33.0	41.0
36-37	38.14214619861387	40.0	38.0	41.0	33.0	41.0
38-39	38.15379483541439	40.0	38.0	41.0	33.0	41.0
40-41	38.159738507442654	40.0	38.0	41.0	33.0	41.0
42-43	38.15757405193969	40.0	38.0	41.0	33.0	41.0
44-45	38.11212659911175	40.0	37.5	41.0	33.0	41.0
46-47	38.113271408177724	40.0	37.0	41.0	33.0	41.0
48-49	38.09520713601381	40.0	37.0	41.0	33.0	41.0
50-51	38.02383421756831	40.0	37.0	41.0	33.0	41.0
52-53	37.99508497861747	40.0	37.0	41.0	33.0	41.0
54-55	37.91893842009616	40.0	37.0	41.0	33.0	41.0
56-57	37.80425042742189	40.0	37.0	41.0	32.0	41.0
58-59	37.68912733625871	39.5	36.0	41.0	32.0	41.0
60-61	37.50504152535842	39.0	36.0	41.0	32.0	41.0
62-63	37.24738752296737	39.0	36.0	40.5	32.0	41.0
64-65	36.9153839102477	38.5	35.0	40.0	31.5	41.0
66-67	36.54504834698176	37.5	35.0	39.5	31.0	41.0
68-69	36.12834511597137	37.0	35.0	39.0	31.0	41.0
70-71	35.74224982632384	36.5	35.0	39.0	31.0	40.0
72-73	35.319518310442	36.0	34.0	37.5	31.0	39.0
74-75	34.94318778216247	35.5	34.0	37.0	31.0	39.0
76-77	32.62106588597979	33.5	31.0	35.5	27.5	36.5
78-79	34.1422550592249	35.0	33.5	36.0	30.0	37.0
80-81	34.17491996413307	35.0	34.0	36.0	31.0	37.0
82-83	34.02687401266006	35.0	34.0	35.5	31.0	36.5
84-85	33.898201060127164	35.0	34.0	35.0	31.0	36.0
86-87	33.727527682137264	35.0	34.0	35.0	30.5	36.0
88-89	33.64734814421489	35.0	34.0	35.0	30.0	36.0
90-91	33.65626121195247	35.0	34.0	35.0	31.0	35.5
92-93	33.62767201023176	35.0	34.0	35.0	31.0	35.0
94-95	33.62002858669254	35.0	34.0	35.0	31.0	35.0
96-97	33.66401485705039	35.0	34.0	35.0	31.0	35.0
98-99	33.7292891429264	35.0	34.0	35.0	31.0	35.0
100-101	33.32498576856299	34.5	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1311	1	-0.7022783335467722
1311	2	-0.5784048294449491
1311	3	-0.02642092687297648
1311	4	0.2601987596222699
1311	5	1.116354760659739
1311	6	0.34669893237834515
1311	7	0.12515147921676117
1311	8	0.04956295720882764
1311	9	0.011052909085876195
1311	10-11	0.3268863011184351
1311	12-13	0.04787665052894141
1311	14-15	0.07414118142413884
1311	16-17	-0.11615706188526076
1311	18-19	0.004240260549423169
1311	20-21	-0.13789438089132489
1311	22-23	-0.11113683032500177
1311	24-25	-0.2050498553937672
1311	26-27	-0.09512959873231353
1311	28-29	-0.1272219171341007
1311	30-31	-0.1456168313005719
1311	32-33	-0.19594269678949416
1311	34-35	-0.13974211183979435
1311	36-37	-0.19058155634647278
1311	38-39	-0.328590470601398
1311	40-41	-0.18105875110869363
1311	42-43	-0.15127674317675854
1311	44-45	-0.10101018250484373
1311	46-47	-0.07940803003123165
1311	48-49	-0.22215150134969264
1311	50-51	-0.1020809555621014
1311	52-53	-0.168945239825959
1311	54-55	-0.15270475577928977
1311	56-57	-0.16806697894805467
1311	58-59	-0.22620513275201404
1311	60-61	-0.25056862574150784
1311	62-63	-0.15413426957437792
1311	64-65	-0.19497215650286392
1311	66-67	-0.07105797921585122
1311	68-69	-0.08358391711754365
1311	70-71	-0.1452923315920316
1311	72-73	-0.13977164164853662
1311	74-75	-0.044902746482641476
1311	76-77	-0.1349269083562703
1311	78-79	-0.04811251395310734
1311	80-81	-0.06286684098356687
1311	82-83	-0.015276902377614476
1311	84-85	-0.029571601784617485
1311	86-87	-0.030115300180547422
1311	88-89	-0.08540291867913652
1311	90-91	-0.0590232060941247
1311	92-93	-0.09159873887470127
1311	94-95	-0.11663053949327917
1311	96-97	-0.05644436365874839
1311	98-99	-0.06874519951510649
1311	100-101	-0.08011276893371644
2103	1	0.10128703386284954
2103	2	0.6808310673335889
2103	3	0.3488776265687612
2103	4	0.5277852045325773
2103	5	1.1731351014325213
2103	6	0.3661931317031417
2103	7	0.10838893282613071
2103	8	0.031018451607657482
2103	9	0.022823931464884595
2103	10-11	0.3323661526355437
2103	12-13	0.0031651311676270666
2103	14-15	0.008003221036517516
2103	16-17	-0.10159247323518628
2103	18-19	-0.22015645071699907
2103	20-21	-0.22874867652457453
2103	22-23	-0.12274652140250453
2103	24-25	-0.25554765314971206
2103	26-27	-0.17126384428159014
2103	28-29	-0.18385447512400788
2103	30-31	-0.1655858972671851
2103	32-33	-0.1753385094350577
2103	34-35	-0.20951603125466534
2103	36-37	-0.2176286482265084
2103	38-39	-0.22934914271544216
2103	40-41	-0.17370034595924722
2103	42-43	-0.15566241773260714
2103	44-45	-0.21811906996699548
2103	46-47	-0.20940166244078995
2103	48-49	-0.18240795989895275
2103	50-51	-0.14873308746449254
2103	52-53	-0.21812155861040594
2103	54-55	-0.2147104405635787
2103	56-57	-0.23368977795022516
2103	58-59	-0.1881027664197461
2103	60-61	-0.15396790613579014
2103	62-63	-0.20875208613107787
2103	64-65	-0.1565076133973733
2103	66-67	-0.1187074825061245
2103	68-69	-0.07749275144428935
2103	70-71	-0.05723896058859168
2103	72-73	-0.09509332363086287
2103	74-75	-0.06863651179735797
2103	76-77	-0.2565607480776748
2103	78-79	-0.11511731142413595
2103	80-81	-0.059380213413923855
2103	82-83	-0.027271812558176123
2103	84-85	-0.030369800255883206
2103	86-87	-0.008672027392748305
2103	88-89	-0.014364369477291916
2103	90-91	-0.06181063284961397
2103	92-93	-0.0917684517458568
2103	94-95	-0.05849611166498647
2103	96-97	-0.050423410357716136
2103	98-99	-0.0686645302552904
2103	100-101	-0.117441406503211
1310	1	-1.3229580830143135
1310	2	-0.6522181352233396
1310	3	-0.9450004489264465
1310	4	-0.8749943029560114
1310	5	0.6894248515200729
1310	6	0.115953735327885
1310	7	0.028903737735596735
1310	8	-0.004467554790352324
1310	9	-0.09315494369050725
1310	10-11	0.17337367050201635
1310	12-13	-0.07227915346987857
1310	14-15	-0.10053387094350796
1310	16-17	-0.15631885278362745
1310	18-19	-0.23245824067977594
1310	20-21	-0.32145312749025123
1310	22-23	-0.2775346875231861
1310	24-25	-0.2368603708828516
1310	26-27	-0.22846336432959902
1310	28-29	-0.2063094777397012
1310	30-31	-0.22710106383448903
1310	32-33	-0.2951276791561597
1310	34-35	-0.3199247861104624
1310	36-37	-0.29152809065894303
1310	38-39	-0.30153700639419156
1310	40-41	-0.31193636025440696
1310	42-43	-0.31338322385976625
1310	44-45	-0.3357001883777926
1310	46-47	-0.3441740644199669
1310	48-49	-0.3116728239663047
1310	50-51	-0.3313920743348291
1310	52-53	-0.3266617011909432
1310	54-55	-0.31188656955474414
1310	56-57	-0.2970363308471491
1310	58-59	-0.3382309111979822
1310	60-61	-0.3482322304184109
1310	62-63	-0.3044287472186724
1310	64-65	-0.2796237292030739
1310	66-67	-0.27540687536834696
1310	68-69	-0.32807076559736714
1310	70-71	-0.27635529228402334
1310	72-73	-0.27609169937632316
1310	74-75	-0.24247200046471562
1310	76-77	-0.26563029848441744
1310	78-79	-0.19381145902736563
1310	80-81	-0.1846459920852297
1310	82-83	-0.178442430423722
1310	84-85	-0.2334476446785132
1310	86-87	-0.10430070130125557
1310	88-89	-0.09894646869634016
1310	90-91	-0.09662415213526288
1310	92-93	-0.1345474994684892
1310	94-95	-0.17083250090360735
1310	96-97	-0.15906146119032627
1310	98-99	-0.15565478630723817
1310	100-101	-0.18997885519583946
2102	1	-1.3203136163976659
2102	2	-1.0663010674281672
2102	3	-1.0543859827666928
2102	4	-0.6272172352815346
2102	5	-0.43620789855560105
2102	6	-0.2629384541467843
2102	7	-0.20270402797736864
2102	8	-0.12324065412791185
2102	9	-0.15325950921667442
2102	10-11	0.19044783851519753
2102	12-13	-0.1314371591209138
2102	14-15	-0.07528921881139894
2102	16-17	-0.14819251204718853
2102	18-19	-0.2831696822309411
2102	20-21	-0.3406470733596336
2102	22-23	-0.3366980081190718
2102	24-25	-0.33228659246856296
2102	26-27	-0.3778713503299045
2102	28-29	-0.49180037830252843
2102	30-31	-0.46983533894177043
2102	32-33	-0.4850071236155742
2102	34-35	-0.4402886569118323
2102	36-37	-0.4685576965173226
2102	38-39	-0.4182443390900019
2102	40-41	-0.5072636518344851
2102	42-43	-0.38968488978620286
2102	44-45	-0.4383665394715095
2102	46-47	-0.44574175280949646
2102	48-49	-0.3438238139616132
2102	50-51	-0.42503576924751485
2102	52-53	-0.4029836778739764
2102	54-55	-0.3554621691991926
2102	56-57	-0.4196173127215417
2102	58-59	-0.3742781417784684
2102	60-61	-0.3130248211716804
2102	62-63	-0.38470175190484923
2102	64-65	-0.40825826126076237
2102	66-67	-0.4048497473693402
2102	68-69	-0.39703317056096665
2102	70-71	-0.331351424353322
2102	72-73	-0.3121052310135468
2102	74-75	-0.25483710978409135
2102	76-77	-0.3648756110608389
2102	78-79	-0.24039201993620196
2102	80-81	-0.2638430574979296
2102	82-83	-0.2709379116645607
2102	84-85	-0.23084978267657164
2102	86-87	-0.11727474736785837
2102	88-89	-0.12488868310550316
2102	90-91	-0.17229178769237308
2102	92-93	-0.17738019868114208
2102	94-95	-0.23706743774852868
2102	96-97	-0.22380806821715993
2102	98-99	-0.18724154860571218
2102	100-101	-0.20093848851535512
1309	1	-1.6961731651051366
1309	2	-0.7420416758502952
1309	3	-0.12901316485832126
1309	4	-1.4666455984575393
1309	5	-1.5887136968404363
1309	6	-0.7507636971570477
1309	7	-0.4026745111647685
1309	8	-0.2778926029953084
1309	9	-0.2260620845528436
1309	10-11	0.11983263513149467
1309	12-13	-0.1586005942697497
1309	14-15	-0.18632847281362075
1309	16-17	-0.19314541362880533
1309	18-19	-0.3239548528260201
1309	20-21	-0.37301737888763853
1309	22-23	-0.40854662352591475
1309	24-25	-0.47075470197740543
1309	26-27	-0.38899923447518603
1309	28-29	-0.45691344745617357
1309	30-31	-0.3993260111954058
1309	32-33	-0.4433744592036959
1309	34-35	-0.45541227176691734
1309	36-37	-0.533011385686585
1309	38-39	-0.4632022396110429
1309	40-41	-0.4750010376261429
1309	42-43	-0.480098672621061
1309	44-45	-0.4666362171574079
1309	46-47	-0.4500987779728476
1309	48-49	-0.43241327513072036
1309	50-51	-0.4331032169652502
1309	52-53	-0.48154011291035914
1309	54-55	-0.4639243931284014
1309	56-57	-0.48071483108928703
1309	58-59	-0.4823366913427449
1309	60-61	-0.48013424410783045
1309	62-63	-0.45901829132517946
1309	64-65	-0.4584346385922231
1309	66-67	-0.42483582028270916
1309	68-69	-0.34311783686309383
1309	70-71	-0.36245481611112496
1309	72-73	-0.3765019267499383
1309	74-75	-0.3475388747363226
1309	76-77	-0.6842351882526678
1309	78-79	-0.42497580758986686
1309	80-81	-0.31126165647515336
1309	82-83	-0.3081638659045396
1309	84-85	-0.2922712313674509
1309	86-87	-0.205789466605502
1309	88-89	-0.18422957546502516
1309	90-91	-0.28370051559704734
1309	92-93	-0.29608767607185627
1309	94-95	-0.2981310982594536
1309	96-97	-0.2684349679421345
1309	98-99	-0.23595022012466416
1309	100-101	-0.25040624910499076
2101	1	-1.0432527023628353
2101	2	-0.9008911275056803
2101	3	-0.6809121492895045
2101	4	-1.760529237348237
2101	5	-1.7548161074172626
2101	6	-0.7628732885436023
2101	7	-0.46075812782958536
2101	8	-0.2948521412809981
2101	9	-0.26481083369522906
2101	10-11	0.13773228649706937
2101	12-13	-0.1832818011374684
2101	14-15	-0.2205695113990913
2101	16-17	-0.28420309902217866
2101	18-19	-0.38794502037246303
2101	20-21	-0.5133119088915663
2101	22-23	-0.49279329503769986
2101	24-25	-0.5307656002307937
2101	26-27	-0.45111945487624894
2101	28-29	-0.48633057708357796
2101	30-31	-0.4536469313933864
2101	32-33	-0.5295559075510923
2101	34-35	-0.5086878166990374
2101	36-37	-0.5383559755953442
2101	38-39	-0.501473465951868
2101	40-41	-0.5574617355928098
2101	42-43	-0.5554003777036698
2101	44-45	-0.6084788368380032
2101	46-47	-0.5190290299348277
2101	48-49	-0.4690637163004112
2101	50-51	-0.45972205083241136
2101	52-53	-0.5397675473266474
2101	54-55	-0.533922585400255
2101	56-57	-0.5197635888383232
2101	58-59	-0.4793363672743638
2101	60-61	-0.5401861519484612
2101	62-63	-0.6001010608891519
2101	64-65	-0.49444055236068607
2101	66-67	-0.5618668905470869
2101	68-69	-0.4203467491035795
2101	70-71	-0.3826430516021233
2101	72-73	-0.44569343437052567
2101	74-75	-0.4267343508253987
2101	76-77	-0.4659643054564597
2101	78-79	-0.43326578999667476
2101	80-81	-0.33416797184568736
2101	82-83	-0.3212387704523323
2101	84-85	-0.2912867094680962
2101	86-87	-0.22303643138047846
2101	88-89	-0.24165246479419977
2101	90-91	-0.3173778764685977
2101	92-93	-0.34977692827675355
2101	94-95	-0.3144718047981101
2101	96-97	-0.2884226203569895
2101	98-99	-0.25097577209398025
2101	100-101	-0.2925536584407311
1308	1	-2.3753561288729372
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2108	28-29	0.41794447555012937
2108	30-31	0.37739611405578444
2108	32-33	0.4197325846882407
2108	34-35	0.4383589596627502
2108	36-37	0.43996229657534514
2108	38-39	0.48453907287669296
2108	40-41	0.4162601617403112
2108	42-43	0.43964131393815364
2108	44-45	0.4074818422085471
2108	46-47	0.42259137415167203
2108	48-49	0.42543406224362457
2108	50-51	0.4761698540013768
2108	52-53	0.43472650934351265
2108	54-55	0.42950237958411464
2108	56-57	0.4450177628475842
2108	58-59	0.45352408747606887
2108	60-61	0.4390691887283964
2108	62-63	0.4342609275441802
2108	64-65	0.4055043323889649
2108	66-67	0.3919136542858723
2108	68-69	0.4171698621263076
2108	70-71	0.41361149092807636
2108	72-73	0.338218874905742
2108	74-75	0.35311213137612185
2108	76-77	0.4831635319287457
2108	78-79	0.3491597517920866
2108	80-81	0.2668464324005413
2108	82-83	0.27014947496714115
2108	84-85	0.2765433325765727
2108	86-87	0.35056486189517244
2108	88-89	0.33379419922040654
2108	90-91	0.24166125623946755
2108	92-93	0.27821554912297586
2108	94-95	0.2697658288380964
2108	96-97	0.25526756810335627
2108	98-99	0.20957001720059765
2108	100-101	0.246583673525258
1312	1	1.0146095598340459
1312	2	0.8830639403608487
1312	3	0.07470888888932237
1312	4	0.3195996701952595
1312	5	0.20486399344524386
1312	6	0.19692661890626084
1312	7	0.1725035564419528
1312	8	0.16902049439550382
1312	9	0.19239167372121813
1312	10-11	0.48771393872360136
1312	12-13	0.22741720735194093
1312	14-15	0.32754459246558554
1312	16-17	0.29595376601133694
1312	18-19	0.30687072743737787
1312	20-21	0.3525930777767172
1312	22-23	0.26130530557243503
1312	24-25	0.32996777634254926
1312	26-27	0.3420333922746579
1312	28-29	0.338654701309558
1312	30-31	0.24992487056754698
1312	32-33	0.24171277809963243
1312	34-35	0.34182261087426014
1312	36-37	0.33091230722091325
1312	38-39	0.34937657798270294
1312	40-41	0.30407361852524417
1312	42-43	0.3240732427485682
1312	44-45	0.3442195264196215
1312	46-47	0.3651148428479303
1312	48-49	0.3574945148107531
1312	50-51	0.31115435070289976
1312	52-53	0.36928561361538925
1312	54-55	0.36365520657725625
1312	56-57	0.31743580300585705
1312	58-59	0.3843390693569546
1312	60-61	0.36634930979606395
1312	62-63	0.35933229355272545
1312	64-65	0.35043301244660796
1312	66-67	0.29795522988453627
1312	68-69	0.3044495403368117
1312	70-71	0.29319051962681897
1312	72-73	0.29664850138373566
1312	74-75	0.24496297429048752
1312	76-77	0.4280500268336809
1312	78-79	0.25366850550425823
1312	80-81	0.22925742929032822
1312	82-83	0.22979295719043336
1312	84-85	0.22033062114531532
1312	86-87	0.26418147727639507
1312	88-89	0.28148716917301897
1312	90-91	0.2382112252533446
1312	92-93	0.20751996913798365
1312	94-95	0.2339805920844853
1312	96-97	0.17427204222531856
1312	98-99	0.16619521112523472
1312	100-101	0.19284275653975413
1313	1	1.3222220671254767
1313	2	1.4632473927562302
1313	3	0.7808304796890262
1313	4	0.9164668138691638
1313	5	1.416757206939586
1313	6	0.6370776741576663
1313	7	0.3106682225441446
1313	8	0.17889406935173469
1313	9	0.2053860158953924
1313	10-11	0.4824227507131553
1313	12-13	0.1903051855848119
1313	14-15	0.24970191021401433
1313	16-17	0.2657565307210987
1313	18-19	0.2801913628622543
1313	20-21	0.2848828783976529
1313	22-23	0.277037760884852
1313	24-25	0.2528295000472838
1313	26-27	0.26082864263963756
1313	28-29	0.2588213771398671
1313	30-31	0.23345217289232068
1313	32-33	0.2807744605206537
1313	34-35	0.29130171476504074
1313	36-37	0.28252809889252717
1313	38-39	0.3328893330040401
1313	40-41	0.32241894584518604
1313	42-43	0.2979900932872539
1313	44-45	0.3012894699725024
1313	46-47	0.28648341692417745
1313	48-49	0.28360359312893735
1313	50-51	0.32018417787332254
1313	52-53	0.26487744654723144
1313	54-55	0.24072013028361283
1313	56-57	0.3203468986512519
1313	58-59	0.32299954523124086
1313	60-61	0.30749894076398476
1313	62-63	0.29372591902490086
1313	64-65	0.2621346417146597
1313	66-67	0.25619871181879716
1313	68-69	0.23370368346003545
1313	70-71	0.2010399727466634
1313	72-73	0.23957592661611216
1313	74-75	0.24301588853153078
1313	76-77	0.34675064681605505
1313	78-79	0.2138185969679398
1313	80-81	0.17195615470113523
1313	82-83	0.15229677574983924
1313	84-85	0.1493873270344821
1313	86-87	0.16890185980400219
1313	88-89	0.20060210343750384
1313	90-91	0.16284561646293128
1313	92-93	0.14985468216749354
1313	94-95	0.1762468248879827
1313	96-97	0.1331118566566758
1313	98-99	0.12705387888638597
1313	100-101	0.18411817752730286
1314	1	0.7182043052779008
1314	2	0.7713700214092114
1314	3	0.3960367830823266
1314	4	1.0017614772376362
1314	5	-0.5476726305248931
1314	6	-0.06093535938733652
1314	7	0.05254161463656004
1314	8	0.04879284272752926
1314	9	0.10605274482713867
1314	10-11	0.4705659371744133
1314	12-13	0.18436582577138694
1314	14-15	0.2442448946887481
1314	16-17	0.21790629028582487
1314	18-19	0.181505864448539
1314	20-21	0.2439179016346813
1314	22-23	0.1742540590518189
1314	24-25	0.18048148801564423
1314	26-27	0.20457028509157738
1314	28-29	0.1930984990434581
1314	30-31	0.16275987205789733
1314	32-33	0.2009941981868124
1314	34-35	0.18667636082294337
1314	36-37	0.20572522636819457
1314	38-39	0.20369288536061703
1314	40-41	0.20072902300121598
1314	42-43	0.17393315110498264
1314	44-45	0.251442677497586
1314	46-47	0.20332108226044454
1314	48-49	0.20051823111848677
1314	50-51	0.16627601888536958
1314	52-53	0.21752656918315694
1314	54-55	0.2473429481732552
1314	56-57	0.22440347672009864
1314	58-59	0.22208720738957055
1314	60-61	0.28293226690783513
1314	62-63	0.2234192892499962
1314	64-65	0.22992848851275483
1314	66-67	0.17641728502702847
1314	68-69	0.14887090977436657
1314	70-71	0.1886732091858505
1314	72-73	0.2088133002562671
1314	74-75	0.22349017034807162
1314	76-77	0.30681937391523206
1314	78-79	0.19205321072333703
1314	80-81	0.1587947002309349
1314	82-83	0.14373143830602686
1314	84-85	0.12664082507063057
1314	86-87	0.22282246538685513
1314	88-89	0.2033033521488079
1314	90-91	0.13699402785928783
1314	92-93	0.1375925089298491
1314	94-95	0.17631670650070674
1314	96-97	0.12973147815215924
1314	98-99	0.1142302283691734
1314	100-101	0.14267217439708446
1315	1	-2.245373058395085
1315	2	-0.22163438090796816
1315	3	0.14844201221686149
1315	4	-0.45935073566028706
1315	5	-1.6998132311087915
1315	6	-0.6348094725936448
1315	7	-0.2546590004783198
1315	8	-0.09363375664592155
1315	9	-0.029341019107107513
1315	10-11	0.3567688118339305
1315	12-13	0.1265334250463539
1315	14-15	0.19038615548774374
1315	16-17	0.1567033020004942
1315	18-19	0.16165543757313827
1315	20-21	0.13391839161177188
1315	22-23	0.10157609478881824
1315	24-25	0.128213198972702
1315	26-27	0.1131457992629663
1315	28-29	0.0748969036139755
1315	30-31	0.10279555728635614
1315	32-33	0.02255097623802982
1315	34-35	0.017506651548238494
1315	36-37	0.06709014281129555
1315	38-39	0.07736972520815044
1315	40-41	0.08673394198959272
1315	42-43	0.04400959750561384
1315	44-45	0.09688366618879485
1315	46-47	0.08874135437408626
1315	48-49	0.07202776637098651
1315	50-51	0.1140950698623584
1315	52-53	0.15710900270586592
1315	54-55	0.11689700445600693
1315	56-57	0.12147180881525799
1315	58-59	0.16732847623369196
1315	60-61	0.13600326847233646
1315	62-63	0.08182634893525886
1315	64-65	0.09177772988816457
1315	66-67	0.08496737998235915
1315	68-69	0.052941327377673986
1315	70-71	0.05609126935122788
1315	72-73	0.10971697232685074
1315	74-75	0.07626617506477373
1315	76-77	0.34327438862961657
1315	78-79	0.06211148633338581
1315	80-81	0.04905991103846219
1315	82-83	0.05147011883286012
1315	84-85	0.03130917114463472
1315	86-87	0.09104271537370323
1315	88-89	0.10608010899136389
1315	90-91	0.048656905902319636
1315	92-93	0.052889001117414125
1315	94-95	0.06924746679468541
1315	96-97	0.03892161070579192
1315	98-99	0.04287439537312565
1315	100-101	0.03722523526871413
1316	1	-1.3095545067034848
1316	2	-0.7311587782586528
1316	3	-1.0121390747786982
1316	4	-0.5130671580197799
1316	5	-1.0788189929459904
1316	6	-0.46728717280595333
1316	7	-0.2072984590214304
1316	8	-0.020828963246273702
1316	9	0.038254288742159304
1316	10-11	0.39949049765876055
1316	12-13	0.14133213525305877
1316	14-15	0.18595636395905046
1316	16-17	0.1509904727867024
1316	18-19	0.07404962324806519
1316	20-21	0.026787898334987403
1316	22-23	0.010375892628431416
1316	24-25	0.05906772675466243
1316	26-27	0.006781527752202976
1316	28-29	-0.025580791570462225
1316	30-31	-0.022173766029645492
1316	32-33	-0.012229074351886027
1316	34-35	-0.006936493111524555
1316	36-37	-0.02331324889944142
1316	38-39	-0.0192034041760607
1316	40-41	0.04831842332460212
1316	42-43	0.05734046891804212
1316	44-45	0.016011707831715682
1316	46-47	0.008445418429097629
1316	48-49	-0.03599909607591201
1316	50-51	0.03695162553056264
1316	52-53	0.039677763576094094
1316	54-55	0.02683373881603046
1316	56-57	0.02817714396481108
1316	58-59	0.0013470528451193786
1316	60-61	-0.029045641668076883
1316	62-63	0.022591758214417723
1316	64-65	0.0777301471504046
1316	66-67	0.06987563451074408
1316	68-69	0.035663541433315515
1316	70-71	0.06303328016024068
1316	72-73	0.03749821778903595
1316	74-75	0.015495028546759215
1316	76-77	0.18364097830947657
1316	78-79	-0.010293825510295562
1316	80-81	0.011789612054045051
1316	82-83	0.006354606446521416
1316	84-85	0.02033267669452954
1316	86-87	0.09306774510021398
1316	88-89	0.06181068324128347
1316	90-91	-0.01893463955561714
1316	92-93	-0.0011957139125442495
1316	94-95	0.007200514882448772
1316	96-97	0.0013334177023693883
1316	98-99	0.019244783555549816
1316	100-101	0.054108978539353814
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	51.0
15	2707.0
16	2043.0
17	2559.0
18	1597.0
19	1357.0
20	1450.0
21	1494.0
22	1937.0
23	2951.0
24	6078.0
25	15275.0
26	37746.0
27	83310.0
28	157007.0
29	256622.0
30	374511.0
31	506374.0
32	655838.0
33	844478.0
34	1103132.0
35	1513321.0
36	2208777.0
37	3722450.0
38	4080418.0
39	367314.0
40	1804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.165595806878402	41.63837347662783	13.248164782146512	36.94786593434726
2	8.745423260550622	44.86475445756022	20.876865725445413	25.512956556443743
3	5.90451732874596	18.069879169591292	43.54973012174565	32.47587337991711
4	8.579859230404958	39.36002443696269	12.444894417160947	39.61522191547141
5	9.52321583865042	42.158423505015584	15.44299898134915	32.875361674984845
6	8.19828901880283	46.814566515449314	18.444180864474703	26.542963601273158
7	6.18021232735221	30.208629285812734	38.44984709953971	25.161311287295344
8	6.357841461401575	33.105676609206434	16.414228411411102	44.12225351798089
9	5.992021062988173	56.32332013131739	17.986466678714134	19.698192126980302
10-11	6.172836445711225	45.3198792620465	28.270726794984213	20.23655749725807
12-13	5.084279067291589	57.13298038402971	16.58862982484074	21.194110723837962
14-15	5.811451602189368	45.721370398074576	16.139134170182697	32.32804382955335
16-17	5.616825971982103	55.43236269636106	16.7186545165378	22.23215681511904
18-19	17.810416053748995	31.119275809560083	17.9191207450533	33.15118739163762
20-21	27.84872129360494	32.07794364000349	17.91861799338558	22.154717073005994
22-23	3.640932661148063	45.37507964480288	17.45438836342682	33.52959933062224
24-25	3.505631456021985	44.788355034421116	16.969741588982178	34.73627192057473
26-27	3.5176334252511046	44.956933130569375	29.00422834345972	22.521205100719797
28-29	3.505481819785947	43.773371035806576	16.920301754419622	35.80084538998785
30-31	3.4917695473901667	33.338679388084586	29.051989865979827	34.117561198545424
32-33	3.5573323106342034	45.57244520219357	21.331453282146782	29.538769205025446
34-35	3.547024478847318	45.0739168495489	20.96721241702096	30.411846254582827
36-37	3.5399603217313467	44.83665455480958	21.183885149047956	30.43949997441112
38-39	3.5549228013887504	45.04517225079528	21.399250300768603	30.000654647047366
40-41	3.562090013953717	44.896496916574804	21.209639900705575	30.331773168765896
42-43	3.53789567122002	44.589331253241056	21.37057913731437	30.502193938224554
44-45	3.5333656978006935	44.89106537355387	21.401574295992567	30.17399463265286
46-47	3.564786078536157	44.8277757352331	21.16149937574932	30.445938810481422
48-49	3.527392242240556	44.64313465531125	21.346907675459047	30.48256542698915
50-51	3.5198139053782085	44.79352507582121	21.50003414595241	30.186626872848173
52-53	3.5285125569142086	44.778556378460586	21.3014836636725	30.39144740095271
54-55	3.505070824125087	44.515416135812536	21.47436839618009	30.505144643882286
56-57	3.5109227779504772	44.80047278299924	21.490056402443308	30.19854803660697
58-59	3.5141405302201627	44.76435764337755	21.293725926957165	30.42777589944512
60-61	3.4869313539435045	44.53993444044626	21.481010723028287	30.492123482581945
62-63	3.47976494017808	44.74813025975655	21.578606466488726	30.193498333576642
64-65	3.487890815201685	44.693193910242904	21.39369999027772	30.425215284277684
66-67	3.452226726742887	44.4669787832416	21.547038129851508	30.533756360164006
68-69	3.448155725266312	44.72137425694561	21.5982430850732	30.232226932714873
70-71	3.450593631948192	44.71780221262817	21.363461279007016	30.46814287641662
72-73	3.4174196138751176	44.469532252618905	21.576174790329024	30.536873343176957
74-75	3.4135680444493888	44.71637488741019	21.65175136150296	30.218305706637455
76-77	3.401568238860423	44.730844803894456	21.430843058285344	30.43674389895978
78-79	3.369733423572635	44.5124461496739	21.59821714681248	30.519603279940988
80-81	3.3655771236866716	44.76978271256764	21.673425137477054	30.191215026268637
82-83	3.365267490731763	44.75711557253112	21.4794518944858	30.398165042251318
84-85	3.331924636151619	44.56001081083546	21.625173660444013	30.48289089256891
86-87	3.4658072775558697	44.74874980960578	21.67879615749375	30.106646755344595
88-89	3.37161531405789	44.82152269053033	21.51435634254117	30.29250565287061
90-91	3.3777893166513038	44.59505566091639	21.641197611341546	30.385957411090764
92-93	3.474965721109602	44.80637648816012	21.672014968451727	30.046642822278557
94-95	3.525139757138893	44.84830763485241	21.454328770119595	30.172223837889106
96-97	3.602564794733369	44.68053436087479	21.55394997488459	30.16295086950725
98-99	3.6752776108741605	44.93495366178896	21.58999167740017	29.799777049936704
100-101	3.7890545750558813	44.973995147490555	21.25318416653484	29.983766110918726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3730.0
1	3691.0
2	3117.5
3	2339.0
4	2116.0
5	2167.0
6	2274.0
7	2324.5
8	2290.0
9	2397.0
10	2626.5
11	2834.5
12	3031.0
13	3613.0
14	4632.0
15	5628.0
16	6627.5
17	8215.5
18	11145.5
19	15242.5
20	21604.0
21	33443.0
22	54509.0
23	91326.5
24	150084.0
25	236337.0
26	357430.0
27	515836.5
28	705148.5
29	907526.0
30	1100142.5
31	1253511.0
32	1351463.5
33	1376510.0
34	1322516.5
35	1218976.5
36	1083419.5
37	934276.0
38	785057.5
39	641369.5
40	516363.5
41	414483.0
42	332334.0
43	268345.0
44	222435.0
45	188744.0
46	161205.0
47	140123.5
48	122291.5
49	105585.0
50	93743.0
51	84910.0
52	77508.5
53	73360.5
54	70660.0
55	67771.0
56	65358.5
57	63191.0
58	61160.0
59	58761.0
60	56260.5
61	53376.5
62	49780.5
63	45851.0
64	41423.0
65	37061.0
66	33092.5
67	29268.5
68	25490.5
69	21898.0
70	18595.0
71	15851.0
72	13582.0
73	11579.0
74	9963.5
75	8510.0
76	7351.0
77	6653.0
78	6140.5
79	5548.5
80	5048.5
81	4729.0
82	4469.5
83	4018.0
84	3603.5
85	3498.5
86	3401.5
87	3351.5
88	2903.5
89	2447.0
90	2294.0
91	2143.5
92	1909.5
93	1622.0
94	1477.0
95	1356.0
96	1288.0
97	1256.0
98	1242.0
99	1238.5
100	1238.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011415076438699252
3	0.0
4	0.0016238378900690588
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	6.280966341745997E-6
20-21	2.199046229669268E-5
22-23	0.0
24-25	0.0016070117792076515
26-27	0.0010015267614092087
28-29	2.4297109088939505E-4
30-31	0.004401447557685128
32-33	0.0
34-35	0.003049580092239293
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0011150152805572363
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.9917868470213287E-4
62-63	1.1001352432925755E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.003982012214413357
78-79	0.0
80-81	0.0
82-83	0.0
84-85	4.0070676382654235E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.005038673835052657
94-95	0.001969238386504528
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	7001.0
5-9	10758.0
10-14	8082.0
15-19	9370.0
20-24	22315.0
25-29	73241.0
30-34	131720.0
35-39	152089.0
40-44	134096.0
45-49	126441.0
50-54	116581.0
55-59	108862.0
60-64	107274.0
65-69	109370.0
70-74	126463.0
75-79	122800.0
80-84	125419.0
85-89	116197.0
90-94	142176.0
95-99	270212.0
100-102	1.3932135E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.99293380380226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.20707524226736	85.67964019930989
2	3.4629463771702023	6.2328140817358975
3	0.6835266409126328	1.8453770324635763
4	0.25292227093890474	0.9104486874444878
5	0.10812547184445771	0.48652642151015846
6	0.041597177916822506	0.2246071247216156
7	0.05547685019503166	0.34947671550714565
8	0.02427722449142505	0.17478229252774893
9	0.018501583272427458	0.14985105828309894
>10	0.13622002059388352	2.59605276110481
>50	0.006484474045430836	0.3802287847614316
>100	0.002499993474609948	0.4213386287920369
>500	1.724673985268061E-4	0.1030892096072325
>1k	1.522879460351534E-4	0.29838001072344994
>5k	1.4934152206781483E-5	0.07221750588568442
>10k+	6.983380048943145E-6	0.07516948562175625
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.7611419127738535E-4	0.0	0.0
2	3.8238276113200845E-4	0.0	0.0
3	3.8238276113200845E-4	0.0	6.268569854623089E-6
4	3.8238276113200845E-4	0.0	6.268569854623089E-6
5	3.8238276113200845E-4	0.0	6.268569854623089E-6
6	3.886513309866315E-4	0.0	1.2537139709246178E-5
7	4.011884706958777E-4	0.0	1.2537139709246178E-5
8	4.074570405505008E-4	0.0	1.2537139709246178E-5
9	4.137256104051239E-4	0.0	1.2537139709246178E-5
10-11	4.3253131996899314E-4	0.0	1.2537139709246178E-5
12-13	4.5760559938748553E-4	3.1342849273115445E-6	2.5074279418492356E-5
14-15	4.6700845416942016E-4	6.268569854623089E-6	2.5074279418492356E-5
16-17	4.764113089513548E-4	6.268569854623089E-6	2.5074279418492356E-5
18-19	4.826798788059779E-4	1.2537139709246178E-5	2.5074279418492356E-5
20-21	5.108884431517817E-4	1.2537139709246178E-5	2.5074279418492356E-5
22-23	5.390970074975857E-4	1.2537139709246178E-5	2.5074279418492356E-5
24-25	5.892455663345703E-4	1.2537139709246178E-5	2.5074279418492356E-5
26-27	6.237227005349974E-4	1.2537139709246178E-5	2.5074279418492356E-5
28-29	6.832741141539168E-4	1.2537139709246178E-5	2.5074279418492356E-5
30-31	7.77302661973263E-4	1.2537139709246178E-5	2.5074279418492356E-5
32-33	8.80734064574544E-4	1.2537139709246178E-5	2.5074279418492356E-5
34-35	9.528226179027096E-4	1.2537139709246178E-5	2.5074279418492356E-5
36-37	0.0010280454561581867	1.2537139709246178E-5	2.5074279418492356E-5
38-39	0.0011126711491955983	1.2537139709246178E-5	2.5074279418492356E-5
40-41	0.0012004311271603214	1.2537139709246178E-5	2.5074279418492356E-5
42-43	0.001369682513235145	1.2537139709246178E-5	2.5074279418492356E-5
44-45	0.0015013224801822297	1.2537139709246178E-5	2.5074279418492356E-5
46-47	0.0016705738662570534	1.2537139709246178E-5	2.8208564345803902E-5
48-49	0.0019025109508781076	1.2537139709246178E-5	3.134284927311545E-5
50-51	0.002078030906807554	1.2537139709246178E-5	3.134284927311545E-5
52-53	0.0022410137230277545	1.2537139709246178E-5	3.134284927311545E-5
54-55	0.0024133993940298895	1.2537139709246178E-5	3.134284927311545E-5
56-57	0.0026390679087963205	1.2537139709246178E-5	3.134284927311545E-5
58-59	0.0029086164125451137	1.2537139709246178E-5	3.447713420042699E-5
60-61	0.0032032391957123986	1.2537139709246178E-5	3.7611419127738534E-5
62-63	0.003460250559751945	1.2537139709246178E-5	3.7611419127738534E-5
64-65	0.003758007627846542	1.2537139709246178E-5	4.074570405505008E-5
66-67	0.004024421846668023	1.2537139709246178E-5	5.014855883698471E-5
68-69	0.004325313199689932	1.2537139709246178E-5	5.014855883698471E-5
70-71	0.004713964530676563	1.2537139709246178E-5	5.014855883698471E-5
72-73	0.005265598677883395	1.2537139709246178E-5	5.014855883698471E-5
74-75	0.00606797561927515	1.2537139709246178E-5	5.014855883698471E-5
76-77	0.006791995437484117	1.2537139709246178E-5	5.014855883698471E-5
78-79	0.007594372378875873	1.2537139709246178E-5	5.014855883698471E-5
80-81	0.008443763594177301	1.2537139709246178E-5	5.014855883698471E-5
82-83	0.009308826234115289	1.2537139709246178E-5	5.014855883698471E-5
84-85	0.010418363098383576	1.2537139709246178E-5	5.014855883698471E-5
86-87	0.09630717296150183	1.2537139709246178E-5	5.014855883698471E-5
88-89	0.23355124135861977	1.2537139709246178E-5	5.014855883698471E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAATC	438260	0.0	75.676605	2
AATAAAT	449195	0.0	73.1179	1
TAAATCA	464060	0.0	72.359985	3
AATCATA	496735	0.0	67.9986	5
TCATAAA	502045	0.0	67.6303	7
AAATCAT	496195	0.0	66.85352	4
ATCATAA	518220	0.0	65.97085	6
GTAAATC	4605	0.0	65.929214	2
AGTAAAT	4680	0.0	64.86431	1
CATAAAC	545000	0.0	62.216255	8
ATAAACA	610700	0.0	55.34885	9
AAATCAG	4870	0.0	46.57584	4
GTCCCCA	2070	0.0	44.518673	1
AGAAATC	4635	0.0	44.05822	2
GTCCCCC	1030	0.0	41.226173	1
ATGAATC	2050	0.0	41.212223	2
TGCCCGT	20785	0.0	40.055088	1
AGAGCAC	2595	0.0	38.89196	94-95
TTGCCCG	11855	0.0	37.60954	1
GATAAAT	4965	0.0	37.303276	1
>>END_MODULE