##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2212_lane2_GCCAAT_L002_R1_004.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15922255
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.741175354872787	31.0	28.0	34.0	16.0	34.0
2	29.61616784973924	31.0	30.0	34.0	16.0	34.0
3	30.67290045111743	31.0	30.0	34.0	25.0	34.0
4	33.817711964285785	35.0	35.0	37.0	28.0	37.0
5	33.851834354142085	35.0	35.0	37.0	28.0	37.0
6	34.97066027195985	37.0	35.0	37.0	32.0	37.0
7	35.367132220142125	37.0	35.0	37.0	33.0	37.0
8	35.50419733634073	37.0	35.0	37.0	33.0	37.0
9	37.25372585664766	39.0	37.0	39.0	34.0	39.0
10-11	37.28007713006157	39.0	37.0	39.0	34.0	39.0
12-13	37.36090092197417	39.0	37.0	39.0	34.0	39.0
14-15	38.706714386347045	40.0	38.0	41.0	34.0	41.0
16-17	38.65027817318131	40.0	38.0	41.0	34.0	41.0
18-19	38.6773173818692	40.0	38.0	41.0	34.0	41.0
20-21	38.704639062733	40.0	38.0	41.0	34.0	41.0
22-23	38.684331981365986	40.0	38.0	41.0	34.0	41.0
24-25	38.66013171444809	40.0	38.0	41.0	34.0	41.0
26-27	38.56963086035863	40.0	38.0	41.0	34.0	41.0
28-29	38.29333124004897	40.0	38.0	41.0	33.5	41.0
30-31	38.45368959556162	40.0	38.0	41.0	34.0	41.0
32-33	38.24613211880033	40.0	38.0	41.0	34.0	41.0
34-35	38.237061621259	40.0	38.0	41.0	33.0	41.0
36-37	38.2560091180698	40.0	38.0	41.0	33.0	41.0
38-39	38.26080798620128	40.0	38.0	41.0	33.0	41.0
40-41	38.25386607036544	40.0	38.0	41.0	33.0	41.0
42-43	38.25857069288925	40.0	38.0	41.0	33.0	41.0
44-45	38.23637238588067	40.0	38.0	41.0	33.0	41.0
46-47	38.215001734211455	40.0	38.0	41.0	33.0	41.0
48-49	38.20030267075043	40.0	37.5	41.0	33.0	41.0
50-51	38.16916018854247	40.0	37.0	41.0	33.0	41.0
52-53	38.111884430475925	40.0	37.0	41.0	33.0	41.0
54-55	37.99828935343254	40.0	37.0	41.0	33.0	41.0
56-57	37.880210143921474	40.0	37.0	41.0	33.0	41.0
58-59	37.80130633503147	40.0	36.5	41.0	32.5	41.0
60-61	37.61761589186946	39.0	36.0	41.0	32.0	41.0
62-63	37.35194390627704	39.0	36.0	41.0	32.0	41.0
64-65	37.05144903923308	39.0	35.0	40.0	32.0	41.0
66-67	36.644552009857094	37.5	35.0	39.5	31.5	41.0
68-69	36.238502422290665	37.0	35.0	39.0	31.0	41.0
70-71	35.820495726274004	36.5	35.0	39.0	31.0	40.0
72-73	35.40361193198418	36.0	34.0	37.5	31.0	39.0
74-75	35.01084071153021	35.5	34.0	37.0	31.0	39.0
76-77	32.98725824284401	34.0	32.0	35.5	27.5	37.0
78-79	34.21965134326781	35.0	33.5	36.0	30.5	37.0
80-81	34.21603815738789	35.0	34.0	36.0	31.0	37.0
82-83	34.05409108358182	35.0	34.0	35.5	31.0	36.5
84-85	33.921229716785106	35.0	34.0	35.0	31.0	36.0
86-87	33.83758715565284	35.0	34.0	35.0	31.0	36.0
88-89	33.73750761884308	35.0	34.0	35.0	31.0	36.0
90-91	33.70808321851129	35.0	34.0	35.0	31.0	35.5
92-93	33.66228065396071	35.0	34.0	35.0	31.0	35.0
94-95	33.67185946860179	35.0	34.0	35.0	31.0	35.0
96-97	33.67742010400078	35.0	34.0	35.0	31.0	35.0
98-99	33.74158292626734	35.0	34.0	35.0	31.0	35.0
100-101	33.36200421515691	34.5	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	-1.9244751812057963
2201	2	-1.6878880482020122
2201	3	-1.4703587615363816
2201	4	-0.8797633944353436
2201	5	-1.293007791607927
2201	6	-0.46126358929220856
2201	7	-0.15241522695298215
2201	8	-0.13161820189493767
2201	9	-0.061554281376047015
2201	10-11	-0.010238795009165358
2201	12-13	-0.019857983254837563
2201	14-15	-0.05848354653794274
2201	16-17	-0.10642627895733625
2201	18-19	-0.10457547495665409
2201	20-21	-0.20309162198012132
2201	22-23	-0.19635114147716592
2201	24-25	-0.17275129677791057
2201	26-27	-0.18724075887171665
2201	28-29	-0.1695123529445084
2201	30-31	-0.14404478638844864
2201	32-33	-0.1587814843777835
2201	34-35	-0.15472555857526515
2201	36-37	-0.23787157089274302
2201	38-39	-0.24956795918592434
2201	40-41	-0.1812980445558594
2201	42-43	-0.16565380762833115
2201	44-45	-0.1842879859301476
2201	46-47	-0.20093118459388393
2201	48-49	-0.2333171211048608
2201	50-51	-0.21091282785345555
2201	52-53	-0.17065061552340666
2201	54-55	-1.4155276858879446
2201	56-57	-1.2135144399245945
2201	58-59	-0.38367100410248867
2201	60-61	-0.1661317100481412
2201	62-63	-0.18701619048896134
2201	64-65	-0.14370930088930578
2201	66-67	-0.16161576751541418
2201	68-69	-0.1668346521035602
2201	70-71	-0.11635826583781039
2201	72-73	-0.13752885235955858
2201	74-75	-0.13320656784500784
2201	76-77	-0.3170929122265491
2201	78-79	-0.12808623230909433
2201	80-81	-0.07897257850046202
2201	82-83	-0.14991773472097947
2201	84-85	-0.12458795558426061
2201	86-87	-0.10407813336136229
2201	88-89	-0.1552725518446536
2201	90-91	-0.12278915708761673
2201	92-93	-0.12021366971041658
2201	94-95	-0.10270562264479821
2201	96-97	-0.05170508763458059
2201	98-99	-0.06004485165941986
2201	100-101	-0.08111542280696682
2202	1	-1.5872242768684934
2202	2	-2.102629113864566
2202	3	-1.4856429920511331
2202	4	-1.7385464724105617
2202	5	-1.865424058864516
2202	6	-0.8068541875458308
2202	7	-0.41445048983310784
2202	8	-0.19308549335036673
2202	9	-0.1374697951467141
2202	10-11	-0.10319018189501605
2202	12-13	-0.17274959819616242
2202	14-15	-0.1991115952891107
2202	16-17	-0.27634288705264254
2202	18-19	-0.3290168709457646
2202	20-21	-0.4345388880558261
2202	22-23	-0.2664831157562588
2202	24-25	-0.2591215891359937
2202	26-27	-0.3018183117569677
2202	28-29	-0.3306848148652932
2202	30-31	-0.29978005014352505
2202	32-33	-0.3625431211050909
2202	34-35	-0.334253855735021
2202	36-37	-0.3635703921117752
2202	38-39	-0.33517287361690506
2202	40-41	-0.2622429457235995
2202	42-43	-0.2827084681655023
2202	44-45	-0.360665587957115
2202	46-47	-0.372305835842198
2202	48-49	-0.3090514868907803
2202	50-51	-0.28098592159987135
2202	52-53	-0.2596942805330471
2202	54-55	-0.24003054329617157
2202	56-57	-0.2380100811994268
2202	58-59	-0.3303688889949754
2202	60-61	-0.3038590728820125
2202	62-63	-0.26977374582122593
2202	64-65	-0.28778129264910746
2202	66-67	-0.27649774954416273
2202	68-69	-0.2987043781762111
2202	70-71	-0.2937630560802589
2202	72-73	-0.33107085366587086
2202	74-75	-0.27159811207601336
2202	76-77	-0.3461832679172616
2202	78-79	-0.28792600005608904
2202	80-81	-0.21992839715435508
2202	82-83	-0.22923937982758247
2202	84-85	-0.20110010154544256
2202	86-87	-0.20204692762003873
2202	88-89	-0.24776643709162727
2202	90-91	-0.224015358458324
2202	92-93	-0.20376794634596251
2202	94-95	-0.2109161123183796
2202	96-97	-0.16581127089849446
2202	98-99	-0.1426033901309438
2202	100-101	-0.14692820403697482
2203	1	-3.6801452538459394
2203	2	-4.029440502830266
2203	3	-2.6195969744545806
2203	4	-1.934652320743016
2203	5	-2.100495182042053
2203	6	-0.9091174165580043
2203	7	-0.4468732778697486
2203	8	-0.27112775347988105
2203	9	-0.20093274831597085
2203	10-11	-0.19914229126380434
2203	12-13	-0.13064200344325627
2203	14-15	-0.20269468156823223
2203	16-17	-0.17865622298449324
2203	18-19	-0.2669449276798517
2203	20-21	-0.38555222346168705
2203	22-23	-0.4270381545837125
2203	24-25	-0.3877637711645221
2203	26-27	-0.4317312989389279
2203	28-29	-0.5200205605499377
2203	30-31	-0.5403201753187972
2203	32-33	-0.5721346789817687
2203	34-35	-0.599873988387273
2203	36-37	-0.5255876280188474
2203	38-39	-0.5339520503648956
2203	40-41	-0.5259410813085594
2203	42-43	-0.5028627803204486
2203	44-45	-0.4913248277800122
2203	46-47	-0.4591524920052521
2203	48-49	-0.5310737579079472
2203	50-51	-0.5240451419874574
2203	52-53	-0.4841786656498357
2203	54-55	-0.373320056507886
2203	56-57	-0.3667205219792038
2203	58-59	-0.46252435355141586
2203	60-61	-0.4442193082200916
2203	62-63	-0.4299044559797096
2203	64-65	-0.4445146242901856
2203	66-67	-0.41228076284847504
2203	68-69	-0.3971894437175223
2203	70-71	-0.3809480136855754
2203	72-73	-0.4061928218840052
2203	74-75	-0.3723461553312859
2203	76-77	-0.6138058919892799
2203	78-79	-0.42568855456869414
2203	80-81	-0.38831401939336985
2203	82-83	-0.3329921068414805
2203	84-85	-0.3142144289638935
2203	86-87	-0.30622697645208774
2203	88-89	-0.2882841675188601
2203	90-91	-0.30606885801096695
2203	92-93	-0.25663979931159275
2203	94-95	-0.2794278322205983
2203	96-97	-0.23691062671148444
2203	98-99	-0.2839513976458008
2203	100-101	-0.2098592202897862
2204	1	-1.2427731900814472
2204	2	-0.8574210447952453
2204	3	-1.123914619256535
2204	4	-1.1470931716617798
2204	5	-1.6278008807373467
2204	6	-0.6957139552341616
2204	7	-0.38045052052981276
2204	8	-0.22911371110456713
2204	9	-0.15690758976748498
2204	10-11	-0.15880595997712277
2204	12-13	-0.26850819569200723
2204	14-15	-0.23825849326251358
2204	16-17	-0.2720545763957247
2204	18-19	-0.3686571126035645
2204	20-21	-0.4470029400014681
2204	22-23	-0.4323362287950019
2204	24-25	-0.42788755081856067
2204	26-27	-0.4553045419698165
2204	28-29	-0.5031415137264048
2204	30-31	-0.44790038515384367
2204	32-33	-0.5593856281561997
2204	34-35	-0.514739743539721
2204	36-37	-0.46082362827200285
2204	38-39	-0.5441576431389876
2204	40-41	-0.530323313444832
2204	42-43	-0.5312536238809784
2204	44-45	-0.49037541408706176
2204	46-47	-0.4570482882855913
2204	48-49	-0.48703606459399396
2204	50-51	-0.45103600833201796
2204	52-53	-0.44219037550215745
2204	54-55	-0.4138230732386674
2204	56-57	-0.3713478232661558
2204	58-59	-0.4430817061008909
2204	60-61	-0.450140499671015
2204	62-63	-0.4098667398054232
2204	64-65	-0.37549376992112116
2204	66-67	-0.34456105765915623
2204	68-69	-0.42254344038219216
2204	70-71	-0.3861421895040138
2204	72-73	-0.30426232873534076
2204	74-75	-0.3419485388548935
2204	76-77	-0.4971800227752894
2204	78-79	-0.33504933051009544
2204	80-81	-0.2818171185228451
2204	82-83	-0.28953999748873827
2204	84-85	-0.24697662767087536
2204	86-87	-0.2379037553399641
2204	88-89	-0.25928259738820003
2204	90-91	-0.23543374265963735
2204	92-93	-0.2025291815955228
2204	94-95	-0.2081307775314727
2204	96-97	-0.2008465348142252
2204	98-99	-0.2083176331028227
2204	100-101	-0.211697607826693
2205	1	-3.581545530140353
2205	2	-3.6110868013927124
2205	3	-1.327177106671332
2205	4	-1.5365152122950434
2205	5	-3.7606006903687863
2205	6	-1.694695870495984
2205	7	-0.8152514158846174
2205	8	-0.45575494871896893
2205	9	-0.4110335998426393
2205	10-11	-0.3361867262080622
2205	12-13	-0.3267567333418384
2205	14-15	-0.38422312587967866
2205	16-17	-0.37725131465180795
2205	18-19	-0.45660917548278945
2205	20-21	-0.5819688355981469
2205	22-23	-0.6744190672220469
2205	24-25	-0.7250275464689722
2205	26-27	-0.6880382513693846
2205	28-29	-0.8314747600421342
2205	30-31	-0.7140822239464342
2205	32-33	-0.8409489133447821
2205	34-35	-0.9794328408901336
2205	36-37	-0.8808690026820969
2205	38-39	-0.8659185341446118
2205	40-41	-0.8225474377931405
2205	42-43	-0.8249356864083808
2205	44-45	-0.8380114367041998
2205	46-47	-0.7943792632925621
2205	48-49	-0.7357758562815491
2205	50-51	-0.7084455481555523
2205	52-53	-0.7636739449851717
2205	54-55	-0.6955790251964302
2205	56-57	-0.7078490286311023
2205	58-59	-0.7381880236897516
2205	60-61	-0.7670671652882532
2205	62-63	-0.779610215466775
2205	64-65	-0.696898613184608
2205	66-67	-0.704617392442799
2205	68-69	-0.6315730397960664
2205	70-71	-0.7134944675037005
2205	72-73	-0.6204144480306226
2205	74-75	-0.6196968845079667
2205	76-77	-0.6641570314453276
2205	78-79	-0.5760538201570142
2205	80-81	-0.5669567891295486
2205	82-83	-0.6792684278902001
2205	84-85	-0.5929515498663847
2205	86-87	-0.532058464703681
2205	88-89	-0.46339390658227586
2205	90-91	-0.43277109514244927
2205	92-93	-0.422058117063159
2205	94-95	-0.4424832179501976
2205	96-97	-0.46345238676048695
2205	98-99	-0.4064037849309372
2205	100-101	-0.43443974370264016
2206	1	-1.687838149372336
2206	2	-2.6369616687332673
2206	3	-3.5622423502570513
2206	4	-2.506957773989541
2206	5	-2.245250975925167
2206	6	-1.8939863321398818
2206	7	-0.8925677647145136
2206	8	-0.5710062392012958
2206	9	-0.5629298388430612
2206	10-11	-0.44784790322722046
2206	12-13	-0.6153271795917092
2206	14-15	-0.6496066627109656
2206	16-17	-0.4661472617721074
2206	18-19	-0.6233058318151521
2206	20-21	-0.8556797420047104
2206	22-23	-0.9174574030091946
2206	24-25	-0.8493793985968452
2206	26-27	-0.9529923469821071
2206	28-29	-1.036566820764584
2206	30-31	-1.0289848571807312
2206	32-33	-1.191770533568402
2206	34-35	-1.2384112884261995
2206	36-37	-1.0660962129383122
2206	38-39	-1.0890575362192862
2206	40-41	-1.0690451290373488
2206	42-43	-1.0913856099019625
2206	44-45	-1.052814866996279
2206	46-47	-0.9946179455392681
2206	48-49	-0.9953206847950113
2206	50-51	-0.9898885894851333
2206	52-53	-0.9923358386052428
2206	54-55	-0.927049794038382
2206	56-57	-0.8530527993268677
2206	58-59	-0.9598060840780178
2206	60-61	-0.8766388484927958
2206	62-63	-1.0306742715578778
2206	64-65	-0.8563098998368517
2206	66-67	-0.8431187383799283
2206	68-69	-0.7759665055975233
2206	70-71	-0.7682318126500931
2206	72-73	-0.7812826512949798
2206	74-75	-0.8048048922589786
2206	76-77	-0.6119998090619063
2206	78-79	-0.7881150135412369
2206	80-81	-0.6939698099312182
2206	82-83	-0.6540686740916613
2206	84-85	-0.6672290876839924
2206	86-87	-0.6760368457721952
2206	88-89	-0.643657955061876
2206	90-91	-0.6686983984231958
2206	92-93	-0.626650085194278
2206	94-95	-0.572020098079669
2206	96-97	-0.6459988241429002
2206	98-99	-0.606750727331729
2206	100-101	-0.45462679662464467
2207	1	2.6379096665293673
2207	2	2.4790778297970633
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2210	36-37	0.1158059473841817
2210	38-39	0.14838291563097528
2210	40-41	0.08553040309066517
2210	42-43	0.07963985799797513
2210	44-45	0.09701039250466437
2210	46-47	0.0670178042790468
2210	48-49	0.09536662215377589
2210	50-51	0.12044979969874703
2210	52-53	0.10180993012033923
2210	54-55	0.1501583215095863
2210	56-57	0.20288591017327207
2210	58-59	0.12895790034376375
2210	60-61	0.1446536568250636
2210	62-63	0.10101426639857891
2210	64-65	0.09535416288000675
2210	66-67	0.07620260148153335
2210	68-69	0.12062283668810636
2210	70-71	0.09829526923969212
2210	72-73	0.029103436552233575
2210	74-75	0.09909494182387135
2210	76-77	0.21502465327163378
2210	78-79	0.1066257419317651
2210	80-81	0.10331376516959523
2210	82-83	0.10576170405092
2210	84-85	0.10276918767519305
2210	86-87	0.11499349736178743
2210	88-89	0.09645625987558049
2210	90-91	0.12171573366538979
2210	92-93	0.09771637712749737
2210	94-95	0.07410503979855321
2210	96-97	0.10158212021264745
2210	98-99	0.0838280913171019
2210	100-101	0.11286076024128278
2209	1	-0.18603669483209728
2209	2	0.6475309302079992
2209	3	0.8126116832459367
2209	4	0.10238286611929936
2209	5	0.13747899415429288
2209	6	0.18540205328120862
2209	7	0.08140015778977272
2209	8	-0.0018011558765351765
2209	9	0.10136348672525486
2209	10-11	0.07565339186604803
2209	12-13	0.05811082689034208
2209	14-15	0.027491953147794845
2209	16-17	-0.015192887994324167
2209	18-19	-0.03913192679825528
2209	20-21	-0.021995153904200038
2209	22-23	-0.021031289553008037
2209	24-25	-0.008716581408044988
2209	26-27	-0.02333608138768284
2209	28-29	0.04303683147055892
2209	30-31	0.04255467995032802
2209	32-33	0.05581765423426077
2209	34-35	0.08132111765017669
2209	36-37	0.04765368706578954
2209	38-39	0.05117339515457786
2209	40-41	0.057103873012451345
2209	42-43	0.054464990584200734
2209	44-45	0.07488231023608449
2209	46-47	0.025155860897825733
2209	48-49	0.08537563674926929
2209	50-51	0.03290826522589185
2209	52-53	0.06745063098340864
2209	54-55	0.10706968892532842
2209	56-57	0.12679140929326138
2209	58-59	0.07005200314326743
2209	60-61	0.04749944626706082
2209	62-63	0.08481901435659722
2209	64-65	0.03881155361285238
2209	66-67	0.007248838982121697
2209	68-69	0.022945239084890545
2209	70-71	0.052285402244926615
2209	72-73	0.050265342624925324
2209	74-75	0.07077481718702927
2209	76-77	0.12175344991402426
2209	78-79	0.03044190850585693
2209	80-81	0.05826255890477228
2209	82-83	0.0804633792836924
2209	84-85	0.05920407700546093
2209	86-87	0.06417794702986868
2209	88-89	0.054473963970146144
2209	90-91	0.04937781996103041
2209	92-93	0.05099974168426513
2209	94-95	0.03754719285580421
2209	96-97	0.0281410345143982
2209	98-99	0.023433223204094134
2209	100-101	0.038666525080039094
2208	1	-1.4245435145301606
2208	2	-0.5756181194820513
2208	3	-0.6646172223214606
2208	4	-0.6726025694247326
2208	5	-0.13685245923237233
2208	6	-0.006661052541801382
2208	7	-0.0023888156129103777
2208	8	-0.017362468199905834
2208	9	0.020232001039566683
2208	10-11	0.03962301554543757
2208	12-13	0.008062986481689904
2208	14-15	-0.05632040830891327
2208	16-17	0.009290674166841484
2208	18-19	-0.008473746885279354
2208	20-21	-0.015442974946402899
2208	22-23	-0.01307223171815508
2208	24-25	-0.015546919181510077
2208	26-27	-0.0667322335142515
2208	28-29	-0.0986071553019201
2208	30-31	-0.06813761535119056
2208	32-33	-0.056754732644698436
2208	34-35	-0.0720100237610879
2208	36-37	-0.04436553982939273
2208	38-39	-0.05192508581360045
2208	40-41	-0.025547510033973708
2208	42-43	-0.04617740291387662
2208	44-45	-0.05475783763723285
2208	46-47	-0.03205427399506533
2208	48-49	-0.08618293592724058
2208	50-51	-0.029651159808217642
2208	52-53	-0.0818298937451587
2208	54-55	0.013423234615352442
2208	56-57	0.032909407643778366
2208	58-59	0.012007296058264672
2208	60-61	-0.059689850307563574
2208	62-63	-0.0663926334873608
2208	64-65	-0.058691404553378845
2208	66-67	-0.07662342413183865
2208	68-69	-0.044897097851091416
2208	70-71	-0.007846700629649206
2208	72-73	-0.07688762805836546
2208	74-75	-0.0664740531282817
2208	76-77	-0.14050764906864543
2208	78-79	-0.05463970820063224
2208	80-81	-0.04154201119600032
2208	82-83	0.0041598819710984
2208	84-85	-0.01150567442534367
2208	86-87	-0.023773967765372106
2208	88-89	-0.025672391473648304
2208	90-91	-0.011392340332996298
2208	92-93	-0.04381881239339691
2208	94-95	0.0054162689721053425
2208	96-97	-0.030522629082021524
2208	98-99	-0.0024512274766266273
2208	100-101	-0.008515989946609182
2213	1	-1.8853358230981598
2213	2	-1.2562032223608668
2213	3	-1.3964275486965434
2213	4	-1.0485597614231494
2213	5	-1.7923991225793898
2213	6	-1.2089163562102954
2213	7	-0.5387122831324618
2213	8	-0.24548742629363574
2213	9	-0.13306268422623901
2213	10-11	-0.059177549939100516
2213	12-13	-0.025619797831105018
2213	14-15	-0.05363750434818826
2213	16-17	-0.07381758783083825
2213	18-19	-0.07895441240410861
2213	20-21	-0.20861084833683208
2213	22-23	-0.17205523340605566
2213	24-25	-0.20646872906525715
2213	26-27	-0.17230476166479747
2213	28-29	-0.11370776905604885
2213	30-31	-0.1754845854514855
2213	32-33	-0.17256871581788857
2213	34-35	-0.17445407057764584
2213	36-37	-0.15500812379421092
2213	38-39	-0.19600262277912606
2213	40-41	-0.17830455998905137
2213	42-43	-0.14100803760693736
2213	44-45	-0.1598320671709743
2213	46-47	-0.15447715600380008
2213	48-49	-0.18206059337645542
2213	50-51	-0.16598119870933203
2213	52-53	-0.1583951490856137
2213	54-55	-0.06668511583259118
2213	56-57	-0.07517425299926117
2213	58-59	-0.16114649230989642
2213	60-61	-0.1961269067650946
2213	62-63	-0.13058462802491277
2213	64-65	-0.12588771674744237
2213	66-67	-0.1416101355739272
2213	68-69	-0.15847417621388615
2213	70-71	-0.11870549278874165
2213	72-73	-0.10060237292026386
2213	74-75	-0.08905203621994673
2213	76-77	-0.3217442764799543
2213	78-79	-0.13950374864309367
2213	80-81	-0.13001968498419103
2213	82-83	-0.06973676446495602
2213	84-85	-0.13085269483707407
2213	86-87	-0.11769523175339458
2213	88-89	-0.11563811055577133
2213	90-91	-0.115731070587195
2213	92-93	-0.11932288047090367
2213	94-95	-0.10128473206813737
2213	96-97	-0.11473084594603478
2213	98-99	-0.09716366225066508
2213	100-101	-0.09474528756160083
2212	1	-1.854920087545345
2212	2	-2.790921197263792
2212	3	-0.7504209589626178
2212	4	-1.0342468595964363
2212	5	-2.131729790188121
2212	6	-0.9312164520089823
2212	7	-0.3773721761155926
2212	8	-0.18454156291609536
2212	9	-0.21084228621732848
2212	10-11	-0.074229554477661
2212	12-13	-0.13629986970586572
2212	14-15	-0.1844209375034893
2212	16-17	-0.15741406387662948
2212	18-19	-0.11517228019476278
2212	20-21	-0.1741906487473699
2212	22-23	-0.23697163701457669
2212	24-25	-0.24611059033808402
2212	26-27	-0.25178963972505386
2212	28-29	-0.3666480493274733
2212	30-31	-0.30744488543804493
2212	32-33	-0.3438861439466834
2212	34-35	-0.3206431162582746
2212	36-37	-0.3535944572938732
2212	38-39	-0.2872308377844419
2212	40-41	-0.32285452380386204
2212	42-43	-0.27951357299656365
2212	44-45	-0.3026249704603359
2212	46-47	-0.2886158380969803
2212	48-49	-0.29257566048323014
2212	50-51	-0.3393161662880573
2212	52-53	-0.3178521501192151
2212	54-55	-0.22140491820668018
2212	56-57	-0.3257652047106845
2212	58-59	-0.30411535509116305
2212	60-61	-0.32177144564708016
2212	62-63	-0.2828871175529173
2212	64-65	-0.24325634052524236
2212	66-67	-0.26716593101319575
2212	68-69	-0.24708816932431432
2212	70-71	-0.2625985982802419
2212	72-73	-0.21671857166898434
2212	74-75	-0.24703603456727308
2212	76-77	-0.3038371901571608
2212	78-79	-0.25902568298277373
2212	80-81	-0.23737511979815196
2212	82-83	-0.24937617359353936
2212	84-85	-0.20384825312122956
2212	86-87	-0.21776706271880641
2212	88-89	-0.18053358142081777
2212	90-91	-0.2027207406460505
2212	92-93	-0.1887368733665653
2212	94-95	-0.18532904730731303
2212	96-97	-0.21881726791057332
2212	98-99	-0.19121759071946087
2212	100-101	-0.1366356672421034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	118.0
15	5660.0
16	3567.0
17	3490.0
18	2160.0
19	1791.0
20	1858.0
21	1684.0
22	2206.0
23	3419.0
24	6941.0
25	16538.0
26	39137.0
27	83347.0
28	154436.0
29	249054.0
30	361367.0
31	486210.0
32	630219.0
33	808230.0
34	1062242.0
35	1465902.0
36	2168434.0
37	3687387.0
38	4271981.0
39	402980.0
40	1896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.133690862255378	42.13601653785849	13.502735636378137	36.22755696350799
2	8.28726915230162	45.680769776948054	20.74430370874361	25.287657362006712
3	5.514656374862325	18.05996607362506	44.60312120639617	31.82225634511645
4	8.562483832227299	40.217371503034045	12.36879369538842	38.85135096935024
5	9.557547641833493	42.87369377884038	15.285974814137251	32.282783765188874
6	8.158047472123892	47.57344298274149	18.186016142536186	26.082493402598427
7	6.077162604194665	29.87767471616139	39.26596656025175	24.779196119392193
8	6.32320530566103	32.713648233702834	16.1216226099318	44.84152385070433
9	5.939981016633967	56.95334962323829	17.71037234919029	19.396297010937445
10-11	5.909220872703413	45.45459463228465	28.706686674038963	19.929497820972976
12-13	5.015186812037792	57.77829387165468	16.308234121572884	20.898285194734633
14-15	5.68404831879798	45.92717761709125	15.852052900812627	32.53672116329814
16-17	5.520285840760426	56.04356597122536	16.47743527696173	21.958712911052476
18-19	18.25170347805737	30.729086216691904	17.660252573108846	33.35895773214189
20-21	28.755573741622424	31.68297294930287	17.64792306210776	21.913530246966943
22-23	3.564659393681962	45.52122920583862	17.18103599816919	33.733075402310234
24-25	3.4071665029469393	44.92898824233645	16.740569792549095	34.92327546216751
26-27	3.3970932500852298	45.09327229998297	29.262405203906816	22.24722924602498
28-29	3.411852389603244	43.84888395779948	16.697689704665525	36.04157394793175
30-31	3.4050255005698813	32.98711534313249	29.312591997566447	34.295267158731185
32-33	3.4652069760607973	45.70021113413756	21.26272571854932	29.57185617125232
34-35	3.4626366877701007	45.182754159599334	20.897720882677078	30.45688826995349
36-37	3.459872570652805	44.979929277112774	21.109171949524843	30.451026202709574
38-39	3.4671890580843643	45.15973712276819	21.329343608120496	30.043730211026947
40-41	3.473408159946001	45.00778589257089	21.148346354157418	30.370459593325695
42-43	3.4523493046750224	44.68911943538734	21.314980117025	30.54355114291264
44-45	3.441366938345202	45.02144193104996	21.323971573326787	30.213219557278055
46-47	3.4742909374292625	44.9484571130202	21.110352302766184	30.466899646784356
48-49	3.4414427691368843	44.75178791053018	21.291246729050712	30.515522591282224
50-51	3.437273569413546	44.89669157603586	21.43156397587832	30.23447087867227
52-53	3.4488143187434908	44.8687222520581	21.252353075547408	30.430110353651006
54-55	3.4500993513346603	44.5974869769911	21.437852805630932	30.51456086604331
56-57	3.419043240145862	44.888075029594646	21.43824733035011	30.254634399909392
58-59	3.435249534868274	44.8598913470373	21.257133222117048	30.447725895977378
60-61	3.4002787420341263	44.654379848317205	21.421331657178172	30.524009752470498
62-63	3.389509078879777	44.83749842353858	21.51995890434226	30.253033593239387
64-65	3.4100852896001044	44.78966177968366	21.316898033663005	30.48335489705323
66-67	3.3719254133772947	44.579047301384385	21.487321034034174	30.561706251204146
68-69	3.3644109474533233	44.82292605018243	21.54908578739931	30.263577214964933
70-71	3.3733512904341243	44.834413380238225	21.308697273209173	30.483538056118476
72-73	3.3410521102069968	44.58428252059815	21.525296205956646	30.549369163238204
74-75	3.3297856279293887	44.795288093479684	21.6110066127572	30.263919665833726
76-77	3.3197005200813456	44.82004583987892	21.399548162457844	30.460705477581897
78-79	3.2923052526499132	44.61135749243388	21.550024545958053	30.546312708958155
80-81	3.283501459812725	44.86282952815751	21.616067104103276	30.237601907926486
82-83	3.277763808207336	44.866906259367354	21.419641265111288	30.43568866731402
84-85	3.250278607083016	44.6429490332052	21.569358325173024	30.537414034538756
86-87	3.2657288785258842	44.90678193768438	21.649698107600006	30.177791076189735
88-89	3.285451078476984	44.930361873392656	21.44964328801959	30.334543760110773
90-91	3.2971650483726473	44.684818307246246	21.595829840892193	30.422186803488916
92-93	3.390939464264035	44.90728024628893	21.600425405598482	30.10135488384855
94-95	3.4418866565412496	44.95533374660896	21.40185421555582	30.200925381293974
96-97	3.520085278542833	44.773987805335594	21.51411502945391	30.191811886667658
98-99	3.5987261430670903	45.006489746058456	21.54145255025694	29.853331560617512
100-101	3.7134821321238363	45.09626624657906	21.202017774567604	29.988233846729496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4276.0
1	4241.5
2	3693.0
3	2959.5
4	2756.0
5	2813.5
6	2939.5
7	2983.5
8	2912.0
9	2993.5
10	3230.0
11	3445.0
12	3642.5
13	4375.0
14	5497.5
15	6434.0
16	7449.0
17	9114.0
18	12019.5
19	16080.0
20	22536.0
21	34631.0
22	56160.0
23	93551.0
24	153219.0
25	240951.5
26	363190.5
27	523640.0
28	715426.5
29	919535.0
30	1113064.5
31	1268229.0
32	1364134.0
33	1386132.5
34	1329903.5
35	1220943.0
36	1081308.5
37	928295.0
38	777313.0
39	634306.0
40	509319.0
41	406903.5
42	324830.0
43	261865.5
44	215908.0
45	181980.0
46	155383.5
47	134794.0
48	117976.5
49	102779.5
50	91688.5
51	83760.0
52	77381.5
53	73213.5
54	70599.0
55	67930.5
56	65660.5
57	63977.0
58	62313.5
59	60060.0
60	57233.0
61	54075.5
62	50428.5
63	46477.0
64	42564.0
65	38639.0
66	34393.0
67	30199.0
68	26542.5
69	23180.5
70	19864.5
71	17006.5
72	14672.5
73	12753.0
74	11227.5
75	9828.5
76	8749.0
77	8122.5
78	7590.0
79	6968.0
80	6453.5
81	6119.0
82	5874.5
83	5450.5
84	5052.0
85	4924.0
86	4807.5
87	4761.0
88	3952.0
89	3139.5
90	2919.5
91	2698.5
92	2411.5
93	2047.5
94	1903.0
95	1814.5
96	1761.5
97	1732.5
98	1719.0
99	1715.0
100	1715.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0027257469598657626
3	2.4495064087566717E-4
4	0.001557861162156889
5	5.0279437400749176E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.8879470690125205E-4
18-19	0.0
20-21	6.61094854298944E-5
22-23	0.0
24-25	0.0013549904503109087
26-27	2.3350679943400478E-4
28-29	9.167805134192167E-5
30-31	0.0040073954027033225
32-33	0.0
34-35	7.336880023669414E-5
36-37	0.002710303101909863
38-39	1.2865514493371959E-5
40-41	3.874058740932201E-5
42-43	0.0010400242140964687
44-45	0.0
46-47	1.6312999212441025E-5
48-49	8.511163454766358E-5
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.878447839824919E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0029441901952293194
74-75	3.404162030623501E-5
76-77	0.0014079144337952498
78-79	9.598298523332623E-5
80-81	0.0
82-83	1.725111310219625E-5
84-85	3.532138722462823E-4
86-87	0.0
88-89	6.968289301351998E-6
90-91	0.0
92-93	0.009139560385391229
94-95	2.4311653410532112E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	11178.0
5-9	12493.0
10-14	6598.0
15-19	8046.0
20-24	20405.0
25-29	71417.0
30-34	135072.0
35-39	155331.0
40-44	136957.0
45-49	127955.0
50-54	118900.0
55-59	110080.0
60-64	105575.0
65-69	106267.0
70-74	122518.0
75-79	121591.0
80-84	126840.0
85-89	108768.0
90-94	142633.0
95-99	263956.0
100-102	1.3909675E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.02764342442975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.61715685437112	87.03804459397463
2	3.128259787762474	5.695162329988496
3	0.5751422232812554	1.5706152365753963
4	0.27052188574847513	0.9849987901766607
5	0.14522805028982708	0.6609883588503765
6	0.07308911376329229	0.399187787151153
7	0.0489559242092016	0.3119439688700028
8	0.020566663958102274	0.14977079625665637
9	0.015238400456550873	0.12484041148058224
>10	0.10019871345820203	1.8422548036903672
>50	0.0035181012922682183	0.22843194431728425
>100	0.0016700981232196101	0.31915419461566363
>500	2.1231574947675844E-4	0.1336270131536746
>1k	2.276615684502522E-4	0.42381964452530824
>5k	7.300569356712499E-6	0.038065631594069685
>10k+	6.9053987224556785E-6	0.07909449477966833
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.8262328420189224E-4	0.0	0.0
2	2.8262328420189224E-4	0.0	0.0
3	2.88903801628601E-4	0.0	0.0
4	2.951843190553097E-4	0.0	0.0
5	2.951843190553097E-4	0.0	0.0
6	2.951843190553097E-4	0.0	0.0
7	3.014648364820184E-4	0.0	0.0
8	3.2030638876214457E-4	0.0	1.2561034853417433E-5
9	3.32867423615562E-4	0.0	1.2561034853417433E-5
10-11	3.3914794104227073E-4	6.2805174267087164E-6	1.2561034853417433E-5
12-13	3.6112975203575124E-4	9.420776140063075E-6	1.2561034853417433E-5
14-15	3.799713043158774E-4	1.2561034853417433E-5	1.2561034853417433E-5
16-17	4.082336327360666E-4	1.2561034853417433E-5	1.2561034853417433E-5
18-19	4.302154437295471E-4	1.2561034853417433E-5	1.2561034853417433E-5
20-21	4.616180308630907E-4	1.2561034853417433E-5	1.2561034853417433E-5
22-23	4.898803592832799E-4	1.2561034853417433E-5	1.570129356677179E-5
24-25	5.464050161236584E-4	1.570129356677179E-5	1.884155228012615E-5
26-27	5.903686381106194E-4	2.5122069706834866E-5	1.884155228012615E-5
28-29	6.406127775242891E-4	2.5122069706834866E-5	1.884155228012615E-5
30-31	7.316802802115655E-4	2.5122069706834866E-5	1.884155228012615E-5
32-33	8.667114048858029E-4	2.5122069706834866E-5	1.884155228012615E-5
34-35	9.263763204395357E-4	2.5122069706834866E-5	1.884155228012615E-5
36-37	0.0010111633057001035	2.5122069706834866E-5	1.884155228012615E-5
38-39	0.0011147918432407972	3.140258713354358E-5	1.884155228012615E-5
40-41	0.0012466827092016803	3.140258713354358E-5	1.884155228012615E-5
42-43	0.0013628722815957916	3.140258713354358E-5	1.884155228012615E-5
44-45	0.001482202112703257	3.140258713354358E-5	2.5122069706834866E-5
46-47	0.001629794272230912	3.140258713354358E-5	2.5122069706834866E-5
48-49	0.0017993682427520474	3.140258713354358E-5	2.5122069706834866E-5
50-51	0.0019501006609930565	3.140258713354358E-5	2.5122069706834866E-5
52-53	0.002119674631514192	3.140258713354358E-5	2.5122069706834866E-5
54-55	0.0022798278258952644	3.76831045602523E-5	2.5122069706834866E-5
56-57	0.002556170592670448	3.76831045602523E-5	2.5122069706834866E-5
58-59	0.00283879387687234	3.76831045602523E-5	2.5122069706834866E-5
60-61	0.0031245574197875867	3.76831045602523E-5	2.5122069706834866E-5
62-63	0.0033537963058624548	3.76831045602523E-5	2.5122069706834866E-5
64-65	0.003664681918484536	4.396362198696102E-5	2.5122069706834866E-5
66-67	0.003997549342100098	4.396362198696102E-5	2.5122069706834866E-5
68-69	0.004292733661155408	4.396362198696102E-5	2.5122069706834866E-5
70-71	0.004660143930617868	4.396362198696102E-5	2.5122069706834866E-5
72-73	0.005112341185340895	4.396362198696102E-5	2.5122069706834866E-5
74-75	0.00567758775374468	4.396362198696102E-5	2.5122069706834866E-5
76-77	0.006277377167995363	4.396362198696102E-5	2.5122069706834866E-5
78-79	0.007027899000487054	4.396362198696102E-5	2.5122069706834866E-5
80-81	0.007699914365144887	5.024413941366973E-5	2.5122069706834866E-5
82-83	0.008525802406757084	5.024413941366973E-5	2.5122069706834866E-5
84-85	0.009493002090470225	5.024413941366973E-5	3.140258713354358E-5
86-87	0.09487035598914853	5.652465684037845E-5	3.140258713354358E-5
88-89	0.23036623895296238	5.652465684037845E-5	3.140258713354358E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAATC	446005	0.0	75.945076	2
AATAAAT	452320	0.0	73.36046	1
TAAATCA	471690	0.0	72.7405	3
AATCATA	510435	0.0	68.753716	5
TCATAAA	517995	0.0	68.52653	7
AAATCAT	509800	0.0	67.53348	4
ATCATAA	534595	0.0	66.754654	6
CATAAAC	562675	0.0	62.971016	8
ATAAACA	630055	0.0	56.29903	9
AGAAATC	5155	0.0	51.32732	2
AGTAAAT	2900	0.0	49.348038	1
GTAAATC	3015	0.0	49.27064	2
GATAAAT	6215	0.0	48.232037	1
GTGCAAC	2025	0.0	44.59484	2
GTCCCCA	1740	0.0	44.107082	1
CGCTTGT	1950	0.0	43.766693	3
AGTCATA	1440	0.0	43.28114	5
CGTGCAA	2090	0.0	43.20086	1
CCGCTTG	1915	0.0	41.733433	2
AAGAAAT	6165	0.0	41.59335	1
>>END_MODULE