##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2212_lane2_GCCAAT_L002_R1_005.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15909223
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.619693180490337	31.0	28.0	34.0	16.0	34.0
2	29.27355620444655	31.0	28.0	34.0	16.0	34.0
3	30.576872603786196	31.0	30.0	34.0	25.0	34.0
4	33.63113840324186	35.0	33.0	37.0	28.0	37.0
5	33.81904235905842	35.0	35.0	37.0	28.0	37.0
6	34.98906394377367	37.0	35.0	37.0	32.0	37.0
7	35.35392271032753	37.0	35.0	37.0	33.0	37.0
8	35.494274671754056	37.0	35.0	37.0	33.0	37.0
9	37.25848519693402	39.0	37.0	39.0	34.0	39.0
10-11	37.27815555649828	39.0	37.0	39.0	34.0	39.0
12-13	37.34905286379565	39.0	37.0	39.0	34.0	39.0
14-15	38.67875639124759	40.0	38.0	41.0	34.0	41.0
16-17	38.59677613221139	40.0	38.0	41.0	34.0	41.0
18-19	38.66181028684265	40.0	38.0	41.0	34.0	41.0
20-21	38.64150120452524	40.0	38.0	41.0	34.0	41.0
22-23	38.657393787549076	40.0	38.0	41.0	34.0	41.0
24-25	38.66112898956695	40.0	38.0	41.0	34.0	41.0
26-27	38.550735388252505	40.0	38.0	41.0	34.0	41.0
28-29	38.29164944149785	40.0	38.0	41.0	33.5	41.0
30-31	38.41546997507696	40.0	38.0	41.0	34.0	41.0
32-33	38.25330536080436	40.0	38.0	41.0	34.0	41.0
34-35	38.242579659002054	40.0	38.0	41.0	33.5	41.0
36-37	38.25467006175677	40.0	38.0	41.0	33.0	41.0
38-39	38.258028241779094	40.0	38.0	41.0	33.0	41.0
40-41	38.25738963240605	40.0	38.0	41.0	33.0	41.0
42-43	38.25600852108681	40.0	38.0	41.0	33.0	41.0
44-45	38.23533079996554	40.0	38.0	41.0	33.0	41.0
46-47	38.21815778690691	40.0	38.0	41.0	33.0	41.0
48-49	38.19527987856029	40.0	37.5	41.0	33.0	41.0
50-51	38.15629683862486	40.0	37.0	41.0	33.0	41.0
52-53	38.11280595961104	40.0	37.0	41.0	33.0	41.0
54-55	38.03219166420172	40.0	37.0	41.0	33.0	41.0
56-57	37.917614859539626	40.0	37.0	41.0	33.0	41.0
58-59	37.797344366726236	40.0	36.5	41.0	32.5	41.0
60-61	37.58434326245976	39.0	36.0	41.0	32.0	41.0
62-63	37.35566939673777	39.0	36.0	40.5	32.0	41.0
64-65	37.0430074999873	39.0	35.0	40.0	32.0	41.0
66-67	36.64508249301442	37.5	35.0	39.5	31.5	41.0
68-69	36.23479970292321	37.0	35.0	39.0	31.0	41.0
70-71	35.83097593335347	36.5	35.0	39.0	31.0	40.0
72-73	35.42812927763147	36.0	34.0	37.5	31.0	39.0
74-75	35.033174213797444	35.5	34.0	37.0	31.0	39.0
76-77	33.200701110256944	34.5	32.0	35.5	28.0	37.0
78-79	34.244859761326225	35.0	34.0	36.0	30.5	37.0
80-81	34.210963323355124	35.0	34.0	36.0	31.0	37.0
82-83	34.06282593924597	35.0	34.0	35.5	31.0	36.5
84-85	33.939687589067745	35.0	34.0	35.0	31.0	36.0
86-87	33.835811416183546	35.0	34.0	35.0	31.0	36.0
88-89	33.74511478463886	35.0	34.0	35.0	31.0	36.0
90-91	33.698771378150795	35.0	34.0	35.0	31.0	35.5
92-93	33.65875616538265	35.0	34.0	35.0	31.0	35.0
94-95	33.66608369869364	35.0	34.0	35.0	31.0	35.0
96-97	33.688026514139324	35.0	34.0	35.0	31.0	35.0
98-99	33.75411063374868	35.0	34.0	35.0	31.0	35.0
100-101	33.361647608288024	34.5	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2302	1	-3.392704954358315
2302	2	-5.2439838900614255
2302	3	-2.20824667998021
2302	4	-1.517204418276286
2302	5	-2.2325910909565607
2302	6	-1.0419849847505631
2302	7	-0.3846056722740485
2302	8	-0.34295049268644817
2302	9	-0.3170808122433897
2302	10-11	-0.17045772618856603
2302	12-13	-0.15055933128952148
2302	14-15	-0.20689477804909018
2302	16-17	-0.15865166976086442
2302	18-19	-0.12557088262213512
2302	20-21	-0.13625919815067533
2302	22-23	-0.394182917059517
2302	24-25	-0.28976850294704093
2302	26-27	-0.3996900617310928
2302	28-29	-0.2966747177543567
2302	30-31	-0.3332593822315957
2302	32-33	-0.23036749231348352
2302	34-35	-0.26648863794696354
2302	36-37	-0.30399712002333246
2302	38-39	-0.272080637343997
2302	40-41	-0.3459618347606721
2302	42-43	-0.24853034940177565
2302	44-45	-0.28512540790912055
2302	46-47	-0.2878589497761368
2302	48-49	-0.29577329063220503
2302	50-51	-0.2709746676126059
2302	52-53	-0.32104668468704034
2302	54-55	-0.3665696186588221
2302	56-57	-0.345364886232133
2302	58-59	-0.4452957130544206
2302	60-61	-0.4670409706613725
2302	62-63	-0.3756464662109593
2302	64-65	-0.3429939202991861
2302	66-67	-0.37391837151232465
2302	68-69	-0.28468096208418814
2302	70-71	-0.32731792395418324
2302	72-73	-0.2828733423637928
2302	74-75	-0.3493582516788294
2302	76-77	-0.4445447372068969
2302	78-79	-0.3062099370495588
2302	80-81	-0.24730099967108288
2302	82-83	-0.30970016695989244
2302	84-85	-0.33040664351882043
2302	86-87	-0.2744943836012794
2302	88-89	-0.2563838134442946
2302	90-91	-0.20383917025504417
2302	92-93	-0.32950450132672415
2302	94-95	-0.312596486023061
2302	96-97	-0.28120497772304276
2302	98-99	-0.16720479617560358
2302	100-101	-0.19326993704282103
2306	1	-2.7889967037410344
2306	2	-2.912058114931792
2306	3	-0.9840020268877545
2306	4	-1.381144019244715
2306	5	-2.031949456781007
2306	6	-0.8882613793798697
2306	7	-0.3761275740850536
2306	8	-0.21602392786967783
2306	9	-0.167381775556251
2306	10-11	-0.076583025743588
2306	12-13	-0.16572912102961368
2306	14-15	-0.13961088446841785
2306	16-17	-0.14306500716242
2306	18-19	-0.22962166919419502
2306	20-21	-0.19195113626711446
2306	22-23	-0.1836364289320258
2306	24-25	-0.3291009384534931
2306	26-27	-0.3195018549909747
2306	28-29	-0.4229255099628233
2306	30-31	-0.3581181907188693
2306	32-33	-0.36265202924629136
2306	34-35	-0.35996052583168137
2306	36-37	-0.3402502787548869
2306	38-39	-0.34842514578686234
2306	40-41	-0.3549260726341146
2306	42-43	-0.3217523156163793
2306	44-45	-0.3768739094378688
2306	46-47	-0.40380521571218964
2306	48-49	-0.332274674824653
2306	50-51	-0.3635619078510075
2306	52-53	-0.34063793461516667
2306	54-55	-0.30228959713108594
2306	56-57	-0.33790534025566643
2306	58-59	-0.3111528937185639
2306	60-61	-0.2926695115551894
2306	62-63	-0.3059277780992673
2306	64-65	-0.29050804545944686
2306	66-67	-0.3109595473679079
2306	68-69	-0.3218088357145632
2306	70-71	-0.31040363130692583
2306	72-73	-0.2871684016154745
2306	74-75	-0.30031314329725944
2306	76-77	-0.5197661534054205
2306	78-79	-0.2755292607986064
2306	80-81	-0.2494667172360039
2306	82-83	-0.2089778304507135
2306	84-85	-0.21253151629252187
2306	86-87	-0.19667263693361292
2306	88-89	-0.19943162168072348
2306	90-91	-0.21579044464971275
2306	92-93	-0.20559652916207938
2306	94-95	-0.1850657904153934
2306	96-97	-0.21330366532098566
2306	98-99	-0.19044126255673177
2306	100-101	-0.16407257299278655
2303	1	-2.909663423263911
2303	2	-1.778025900755157
2303	3	-0.6200946725313905
2303	4	-2.859774191122245
2303	5	-3.007569401679177
2303	6	-1.3688885545042595
2303	7	-0.6730115845431186
2303	8	-0.3644956458799413
2303	9	-0.26007290039103026
2303	10-11	-0.20331189148066642
2303	12-13	-0.18975893423331058
2303	14-15	-0.2860502474450044
2303	16-17	-0.3842770712626802
2303	18-19	-0.4063288075294196
2303	20-21	-0.5006140291915031
2303	22-23	-0.38369687775697514
2303	24-25	-0.47800558713200303
2303	26-27	-0.4666778778126073
2303	28-29	-0.4797606429857666
2303	30-31	-0.5456965558339348
2303	32-33	-0.5737505186206917
2303	34-35	-0.5391870025628975
2303	36-37	-0.46512218346584433
2303	38-39	-0.5312943892274831
2303	40-41	-0.4140344589598044
2303	42-43	-0.40542937757657427
2303	44-45	-0.43753099088946357
2303	46-47	-0.4155463998234552
2303	48-49	-0.4937900995456701
2303	50-51	-0.43988089103362427
2303	52-53	-0.43684586925139257
2303	54-55	-0.39709058651840934
2303	56-57	-0.38874093335573434
2303	58-59	-0.4438946333778162
2303	60-61	-0.40097673618420515
2303	62-63	-0.44712844288110887
2303	64-65	-0.4231832604315855
2303	66-67	-0.47301971847854674
2303	68-69	-0.3873815353174379
2303	70-71	-0.44597367787958575
2303	72-73	-0.41106536886558587
2303	74-75	-0.42893039374750686
2303	76-77	-0.594332559713088
2303	78-79	-0.4073007481211235
2303	80-81	-0.37443291384775534
2303	82-83	-0.3739038138327331
2303	84-85	-0.3566892480526036
2303	86-87	-0.3274137625801927
2303	88-89	-0.3119417646990925
2303	90-91	-0.34181914315054485
2303	92-93	-0.30977347243486264
2303	94-95	-0.2517895635333929
2303	96-97	-0.2537577944137084
2303	98-99	-0.2209368094017421
2303	100-101	-0.21155034475505374
2307	1	-2.306601647642754
2307	2	-3.5268469096443553
2307	3	-3.2924611260820846
2307	4	-2.934306348075829
2307	5	-3.3716526972704983
2307	6	-1.861685570064978
2307	7	-0.8008154537665177
2307	8	-0.4440045701206117
2307	9	-0.4099468766305634
2307	10-11	-0.3381850832755475
2307	12-13	-0.35939370141272775
2307	14-15	-0.39757910104558647
2307	16-17	-0.4368122686205851
2307	18-19	-0.5953803415517314
2307	20-21	-0.8039520980110026
2307	22-23	-0.6427249865424756
2307	24-25	-0.6544208008158563
2307	26-27	-0.6486935348052967
2307	28-29	-0.831051382484425
2307	30-31	-0.904135495624125
2307	32-33	-1.0260531231311987
2307	34-35	-1.0330975291246958
2307	36-37	-0.9129292241189049
2307	38-39	-0.8644034850150177
2307	40-41	-0.8647274591234577
2307	42-43	-0.9035391439435116
2307	44-45	-0.8608283823916665
2307	46-47	-0.8411730477632275
2307	48-49	-0.7974563287887477
2307	50-51	-0.825934869336109
2307	52-53	-0.8779860282746412
2307	54-55	-0.7632284055838525
2307	56-57	-0.8252279921797196
2307	58-59	-0.8319172973399489
2307	60-61	-0.8066010412225779
2307	62-63	-0.7750125940025328
2307	64-65	-0.7970474005905359
2307	66-67	-0.8244220021084772
2307	68-69	-0.7384876144694275
2307	70-71	-0.7030378584462085
2307	72-73	-0.7440983979427926
2307	74-75	-0.6896088831360387
2307	76-77	-1.0883526241217893
2307	78-79	-0.6937485510460348
2307	80-81	-0.5845044841325375
2307	82-83	-0.5686749149170751
2307	84-85	-0.5922899712806924
2307	86-87	-0.5218790374013622
2307	88-89	-0.5646053281578602
2307	90-91	-0.5968439390243745
2307	92-93	-0.5752547745720094
2307	94-95	-0.5032549053195297
2307	96-97	-0.5029825915800359
2307	98-99	-0.5194426245942552
2307	100-101	-0.48959274048463186
2304	1	3.5177476758431503
2304	2	3.3155865785396443
2304	3	2.087184338207024
2304	4	2.291897488167308
2304	5	2.3813684304740903
2304	6	1.2009634320932676
2304	7	0.6211377338632147
2304	8	0.3028396648150533
2304	9	0.26278299011257644
2304	10-11	0.20986944723306777
2304	12-13	0.2261304716515511
2304	14-15	0.22696234984968555
2304	16-17	0.26128360642406534
2304	18-19	0.4139328291196094
2304	20-21	0.6343192877273154
2304	22-23	0.6273191042544113
2304	24-25	0.5559585454039251
2304	26-27	0.6201671204613675
2304	28-29	0.6752224127515092
2304	30-31	0.6842301004091311
2304	32-33	0.763917074600954
2304	34-35	0.7668654174537011
2304	36-37	0.7620723160350806
2304	38-39	0.718787156217104
2304	40-41	0.6931249751770352
2304	42-43	0.7335679629443277
2304	44-45	0.7071433973355212
2304	46-47	0.6581182775616838
2304	48-49	0.673933664717147
2304	50-51	0.6752391582795525
2304	52-53	0.6330318534087453
2304	54-55	0.6345246917983545
2304	56-57	0.6504028668587694
2304	58-59	0.6864879099031214
2304	60-61	0.6967059026828011
2304	62-63	0.6662861951396479
2304	64-65	0.6394881084212685
2304	66-67	0.6008820250232603
2304	68-69	0.6042622998707046
2304	70-71	0.5509384502914401
2304	72-73	0.5005367364472804
2304	74-75	0.5117552309747424
2304	76-77	0.5769267060610161
2304	78-79	0.51781668835293
2304	80-81	0.41257412517391856
2304	82-83	0.423385202281402
2304	84-85	0.41943555729188375
2304	86-87	0.4078840288845811
2304	88-89	0.37680069979051467
2304	90-91	0.39162766434067464
2304	92-93	0.366642614659888
2304	94-95	0.37354122013622515
2304	96-97	0.3646779565882454
2304	98-99	0.3272850166827581
2304	100-101	0.2821747367557421
2301	1	3.2817378112023228
2301	2	3.169683930588029
2301	3	2.0272507396950274
2301	4	1.3462872415469036
2301	5	2.1294117497175407
2301	6	1.0657174685994022
2301	7	0.5353454405216169
2301	8	0.3158994419228236
2301	9	0.33991676242848
2301	10-11	0.22814574305900237
2301	12-13	0.25759404868618674
2301	14-15	0.285060765296528
2301	16-17	0.34356670454939575
2301	18-19	0.3857678120205321
2301	20-21	0.5929594839016161
2301	22-23	0.5491510325810793
2301	24-25	0.5645973370968136
2301	26-27	0.593628596465102
2301	28-29	0.5999989391154372
2301	30-31	0.6662851410277071
2301	32-33	0.7484759987569234
2301	34-35	0.723091803374686
2301	36-37	0.7054738144651864
2301	38-39	0.711831518800409
2301	40-41	0.6686766297154492
2301	42-43	0.6726829264054928
2301	44-45	0.714617281872826
2301	46-47	0.6803968145286916
2301	48-49	0.6329748374132365
2301	50-51	0.6396478446946645
2301	52-53	0.6423474769779673
2301	54-55	0.6132188603623732
2301	56-57	0.5893353696145667
2301	58-59	0.613065708160839
2301	60-61	0.6194956888520551
2301	62-63	0.5961217042920453
2301	64-65	0.557030431318033
2301	66-67	0.5629843894905591
2301	68-69	0.5547199009230397
2301	70-71	0.5121099708708456
2301	72-73	0.5071604893195243
2301	74-75	0.4910712321899098
2301	76-77	0.5738012956418146
2301	78-79	0.5085174396110048
2301	80-81	0.4176417620370074
2301	82-83	0.40338770201012153
2301	84-85	0.37461796703943406
2301	86-87	0.3657675917094281
2301	88-89	0.3165530896654971
2301	90-91	0.31013446786545273
2301	92-93	0.3281832457970708
2301	94-95	0.3180339315535292
2301	96-97	0.3083670377253611
2301	98-99	0.2986085868097419
2301	100-101	0.26238196439224026
2305	1	3.2094242607545382
2305	2	3.15407860476504
2305	3	1.9955856729341122
2305	4	2.038762077009764
2305	5	2.351668501135066
2305	6	1.1531746997912222
2305	7	0.5521930166006541
2305	8	0.2786779639909085
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2313	46-47	-0.10397572023157409
2313	48-49	-0.1200977746880838
2313	50-51	-0.11686906708951028
2313	52-53	-0.11617445910175661
2313	54-55	-0.1480748563834169
2313	56-57	-0.17236230161472577
2313	58-59	-0.12445454398064015
2313	60-61	-0.07509930663588449
2313	62-63	-0.1168988716298145
2313	64-65	-0.12026094742422089
2313	66-67	-0.08015863727748496
2313	68-69	-0.08557232668756143
2313	70-71	-0.10485788765087989
2313	72-73	-0.08923354217321844
2313	74-75	-0.07226085913099212
2313	76-77	-0.08807248031547488
2313	78-79	-0.06497908986337109
2313	80-81	-0.0838521246126902
2313	82-83	-0.053381253799848594
2313	84-85	-0.04834300277517656
2313	86-87	-0.04338708277087733
2313	88-89	-0.06328243601816297
2313	90-91	-0.05520989164642032
2313	92-93	-0.04008032862582667
2313	94-95	-0.03844955814094675
2313	96-97	-0.05622552611975351
2313	98-99	-0.0445592063940623
2313	100-101	-0.04209439936078496
2316	1	-1.2676769970716286
2316	2	-0.969515904294429
2316	3	-1.100085558805926
2316	4	-0.3850443698749615
2316	5	-1.0863034709903587
2316	6	-0.5393747893004033
2316	7	-0.2812999610301645
2316	8	-0.11963909565435671
2316	9	-0.045311087579193554
2316	10-11	0.017700325365233027
2316	12-13	-0.04116135091926054
2316	14-15	-0.01892052703210112
2316	16-17	-0.04783633274325183
2316	18-19	-0.04898018725186404
2316	20-21	-0.1188488033757551
2316	22-23	-0.16310312530280413
2316	24-25	-0.16722075729838082
2316	26-27	-0.16111338086793836
2316	28-29	-0.12866336147195057
2316	30-31	-0.1546800593975135
2316	32-33	-0.14803377682201102
2316	34-35	-0.2276432129206114
2316	36-37	-0.2638250873225658
2316	38-39	-0.20502427987672434
2316	40-41	-0.2727157192938563
2316	42-43	-0.23399169414535237
2316	44-45	-0.2106311912399974
2316	46-47	-0.23972401840465807
2316	48-49	-0.15568603308101103
2316	50-51	-0.21756850101304792
2316	52-53	-0.16430178856671063
2316	54-55	-0.18213673659965224
2316	56-57	-0.15029849840311016
2316	58-59	-0.1672793221954194
2316	60-61	-0.13187040570753084
2316	62-63	-0.14552354428384007
2316	64-65	-0.12741607602632143
2316	66-67	-0.15301925716735099
2316	68-69	-0.17165611606569797
2316	70-71	-0.1808563569859558
2316	72-73	-0.19724859836163233
2316	74-75	-0.10278282878239509
2316	76-77	3.579710587473528E-4
2316	78-79	-0.11803422390604368
2316	80-81	-0.12738024141857807
2316	82-83	-0.11989363715547796
2316	84-85	-0.114340000586445
2316	86-87	-0.11243872621309947
2316	88-89	-0.0852880187766516
2316	90-91	-0.09926836650435433
2316	92-93	-0.10129279121075996
2316	94-95	-0.12880719600194368
2316	96-97	-0.08941910602637648
2316	98-99	-0.06928359469839052
2316	100-101	-0.07410932841154505
2216	1	-2.078808670769206
2216	2	-2.7684451309737916
2216	3	-1.8837266627345386
2216	4	-1.5845556172996176
2216	5	-1.638385226637972
2216	6	-0.7587590570134779
2216	7	-0.3301188664459218
2216	8	-0.15947532405893838
2216	9	-0.026407211713312506
2216	10-11	-0.12379514753471454
2216	12-13	-0.07458322194739253
2216	14-15	-0.08100827818072531
2216	16-17	-0.06740453754864006
2216	18-19	-0.16569320742722482
2216	20-21	-0.4038633340195261
2216	22-23	-0.21931340709854652
2216	24-25	-0.17090029786535865
2216	26-27	-0.23140188599280265
2216	28-29	-0.24976022068193515
2216	30-31	-0.2675880828678956
2216	32-33	-0.293839365836682
2216	34-35	-0.256535679505987
2216	36-37	-0.2780941409895661
2216	38-39	-0.2837464165911001
2216	40-41	-0.21508198430552739
2216	42-43	-0.26262728400423185
2216	44-45	-0.26187267293488503
2216	46-47	-0.3224530665618346
2216	48-49	-0.2448809685417146
2216	50-51	-0.2610503971306031
2216	52-53	-0.22100465268579939
2216	54-55	-0.2931479795095484
2216	56-57	-0.28899552454701194
2216	58-59	-0.24215923378545057
2216	60-61	-0.25274547060729446
2216	62-63	-0.28554699673770756
2216	64-65	-0.28453318899060065
2216	66-67	-0.21820645692272223
2216	68-69	-0.2510427623888418
2216	70-71	-0.17311974428999122
2216	72-73	-0.21163545419606322
2216	74-75	-0.26907761731564506
2216	76-77	-0.276605883997064
2216	78-79	-0.22383741193298334
2216	80-81	-0.19670284980458064
2216	82-83	-0.19039496603657113
2216	84-85	-0.18115237708240528
2216	86-87	-0.1678273673747981
2216	88-89	-0.15517626770134285
2216	90-91	-0.15018486942886256
2216	92-93	-0.14468407344475764
2216	94-95	-0.16798621859059182
2216	96-97	-0.12759560434061967
2216	98-99	-0.14142537585801307
2216	100-101	-0.15625945381886197
2215	1	-2.922824775909884
2215	2	-2.352167105410235
2215	3	-1.8386548163299778
2215	4	-2.309283343081624
2215	5	-1.9704101947629162
2215	6	-0.9558207454372578
2215	7	-0.4648308280995934
2215	8	-0.2592158861497964
2215	9	-0.24759836560877346
2215	10-11	-0.18386499485449548
2215	12-13	-0.18796622366086524
2215	14-15	-0.19379633998364199
2215	16-17	-0.1801170495850073
2215	18-19	-0.2236144897525847
2215	20-21	-0.46051853821635547
2215	22-23	-0.3337799860753705
2215	24-25	-0.25649526222261443
2215	26-27	-0.2700956676307342
2215	28-29	-0.3621850554170791
2215	30-31	-0.3276832035567381
2215	32-33	-0.37946764716595993
2215	34-35	-0.44446162324995697
2215	36-37	-0.47468940572147034
2215	38-39	-0.4166385492305693
2215	40-41	-0.42966872736344897
2215	42-43	-0.4638235304556062
2215	44-45	-0.42932247528477063
2215	46-47	-0.36456081392212525
2215	48-49	-0.36928428260424084
2215	50-51	-0.3495158399258571
2215	52-53	-0.3750483511353835
2215	54-55	-0.38261308714911735
2215	56-57	-0.3115203233694359
2215	58-59	-0.3511853672322829
2215	60-61	-0.3572535973715958
2215	62-63	-0.38228361667893296
2215	64-65	-0.3256120595091829
2215	66-67	-0.3432696833648379
2215	68-69	-0.327789809948662
2215	70-71	-0.2934851588030867
2215	72-73	-0.31241758173713663
2215	74-75	-0.26804996824674276
2215	76-77	-0.24770015349071173
2215	78-79	-0.3494914264689797
2215	80-81	-0.3222448818737149
2215	82-83	-0.310989277864131
2215	84-85	-0.25813405755690866
2215	86-87	-0.238635436480493
2215	88-89	-0.20441720651535178
2215	90-91	-0.22703986521397468
2215	92-93	-0.20185813788961582
2215	94-95	-0.26394416425320344
2215	96-97	-0.2472143586704263
2215	98-99	-0.16189473426550904
2215	100-101	-0.19503780533447213
2214	1	-1.3859184063565309
2214	2	-2.066083041509863
2214	3	-0.6927978552286262
2214	4	-1.3216437900514535
2214	5	-2.7759552501970646
2214	6	-1.134637324107949
2214	7	-0.6019757831134314
2214	8	-0.3406102389816752
2214	9	-0.2627303115636579
2214	10-11	-0.23807455275353817
2214	12-13	-0.20181310311385658
2214	14-15	-0.29451616498169386
2214	16-17	-0.28522215353496705
2214	18-19	-0.28734999038915277
2214	20-21	-0.7911233411743765
2214	22-23	-0.5669455464030122
2214	24-25	-0.4662164764778609
2214	26-27	-0.490541557337302
2214	28-29	-0.46417277032333004
2214	30-31	-0.4328679789624701
2214	32-33	-0.4497999176265779
2214	34-35	-0.48518915739116864
2214	36-37	-0.6015088560418391
2214	38-39	-0.48453683943991166
2214	40-41	-0.4990522446097003
2214	42-43	-0.5644038075210389
2214	44-45	-0.5023410681235632
2214	46-47	-0.5075823972384939
2214	48-49	-0.5271677852981682
2214	50-51	-0.5083917130011315
2214	52-53	-0.5246616969210294
2214	54-55	-0.5216052127637738
2214	56-57	-0.49676614612773307
2214	58-59	-0.46987595409408556
2214	60-61	-0.500459864285034
2214	62-63	-0.475361800510413
2214	64-65	-0.47595808157296915
2214	66-67	-0.45906729221648135
2214	68-69	-0.4229475567675891
2214	70-71	-0.4101695780186745
2214	72-73	-0.3499495792322165
2214	74-75	-0.41563783285067757
2214	76-77	-0.3506050386434225
2214	78-79	-0.37071837758988835
2214	80-81	-0.3136804348243061
2214	82-83	-0.31222880087413785
2214	84-85	-0.29102263330315026
2214	86-87	-0.293095053629969
2214	88-89	-0.2807810946823892
2214	90-91	-0.2812345363388715
2214	92-93	-0.25052836349438223
2214	94-95	-0.29941523718747476
2214	96-97	-0.2927099776406479
2214	98-99	-0.2735823265650552
2214	100-101	-0.24803343184571247
2213	1	-2.352498921320457
2213	2	-2.32309877508105
2213	3	-0.9315183325862222
2213	4	0.1806864118676046
2213	5	-1.4816197776618765
2213	6	-0.7596580247803644
2213	7	-0.5698288214912992
2213	8	-0.3319389714264602
2213	9	-0.3954604355152469
2213	10-11	-0.43247874195150615
2213	12-13	-0.4809867444375939
2213	14-15	-0.49047008280163595
2213	16-17	-0.5871670826386151
2213	18-19	-0.6061408142707236
2213	20-21	-0.603453197507136
2213	22-23	-0.6270624414023018
2213	24-25	-0.7176242911494697
2213	26-27	-0.7027768095010245
2213	28-29	-0.7074297067060584
2213	30-31	-0.702900428275349
2213	32-33	-0.9125426043720992
2213	34-35	-0.806726432614127
2213	36-37	-0.7459649210667223
2213	38-39	-0.8108805709033433
2213	40-41	-0.6697732713675961
2213	42-43	-0.689048070210184
2213	44-45	-0.766968016466727
2213	46-47	-0.7270430037540336
2213	48-49	-0.7038596399339241
2213	50-51	-0.6756224111696696
2213	52-53	-0.6328279604663507
2213	54-55	-0.6708914510472042
2213	56-57	-0.6802089549601646
2213	58-59	-0.7662418934370407
2213	60-61	-0.9389844451279714
2213	62-63	-0.6967910066760368
2213	64-65	-0.6635793458910513
2213	66-67	-0.5623181847813257
2213	68-69	-0.6193306863777721
2213	70-71	-0.5704887682375315
2213	72-73	-0.5189266095060603
2213	74-75	-0.45120040131797623
2213	76-77	-0.2484452927063998
2213	78-79	-0.5071206846864342
2213	80-81	-0.5379000392065265
2213	82-83	-0.5152269738525277
2213	84-85	-0.4577291026325341
2213	86-87	-0.5019788187830301
2213	88-89	-0.3955300787580427
2213	90-91	-0.4803285406817892
2213	92-93	-0.4811052556970239
2213	94-95	-0.4104493525334547
2213	96-97	-0.3784854530930062
2213	98-99	-0.4667434957568375
2213	100-101	-0.3616026342145986
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	126.0
15	6369.0
16	3510.0
17	3797.0
18	2079.0
19	1737.0
20	1691.0
21	1623.0
22	2210.0
23	3295.0
24	6801.0
25	16697.0
26	39155.0
27	82803.0
28	154233.0
29	249190.0
30	360377.0
31	487651.0
32	630725.0
33	810903.0
34	1067888.0
35	1480929.0
36	2184705.0
37	3661552.0
38	4239852.0
39	407386.0
40	1939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.240402438258613	41.92161364511642	13.512287809404647	36.325696107220324
2	8.580338271169104	45.300821262816676	20.5687965389387	25.55004392707552
3	5.805166496728109	18.242217414368035	44.01209376685989	31.940522322043968
4	8.74409597952906	39.926289532378	12.390255556655806	38.93935893143713
5	9.700178164111396	42.62984142871979	15.378985441470261	32.29099496569855
6	8.214127912964528	47.34648101975235	18.258481521049607	26.180909546233504
7	6.17153349114681	30.002352225433054	38.998658010712	24.827456272708133
8	6.405944200833096	32.84403578493792	16.22973968411253	44.52028033011646
9	5.994872235165147	56.75502950743395	17.815306803541343	19.43479145385956
10-11	5.967042255400927	45.41322084599947	28.64092837305557	19.978808525544032
12-13	5.080880778772652	57.52044530079212	16.413637966426133	20.985035954009092
14-15	5.758185040253119	45.87764849069656	15.952137284157214	32.41202918489311
16-17	5.599534197806071	55.793203780208536	16.5834386635095	22.023823358475894
18-19	18.141700674582868	30.84528158176618	17.773043365546553	33.2399743781044
20-21	28.485149365277213	31.818210462548606	17.734641805716592	21.961998366457593
22-23	3.6258638181799787	45.46122432280257	17.27346688259764	33.63944497641981
24-25	3.4807615465524986	44.86364014412609	16.831039597772637	34.824558711548775
26-27	3.4668265612381575	45.04929328136444	29.158228985491686	22.32565117190572
28-29	3.477717425250701	43.81774352483045	16.787098193387877	35.91744085653097
30-31	3.476680114947697	33.10133563369108	29.21802952947464	34.20395472188659
32-33	3.536473861625342	45.66466176328484	21.29842521072542	29.500439164364394
34-35	3.5258688568882377	45.12786270284481	20.93289208846443	30.41337635180252
36-37	3.5267129036544445	44.92212290313628	21.127218326243955	30.423945866965322
38-39	3.5334271803695314	45.10441845460378	21.36541450445301	29.996739860573673
40-41	3.54111473449781	44.98017066467963	21.17099208319191	30.30772251763065
42-43	3.5201042492387336	44.63647464723527	21.34382357725367	30.499597526272325
44-45	3.513519994146291	44.96696080640886	21.35050703129883	30.16901216814602
46-47	3.5409946294481123	44.90376074443376	21.119757979107526	30.435486647010595
48-49	3.5066383407037356	44.70099853212149	21.3082717830197	30.48409134415508
50-51	3.4993433107855854	44.85797080913174	21.454915846280272	30.187770033802398
52-53	3.5138636938911527	44.80644445785663	21.273552019083066	30.406139829169156
54-55	3.490398517004558	44.557860328780905	21.462127180678742	30.48961397353579
56-57	3.4829058943425397	44.85294441914293	21.44341088638541	30.220738800129116
58-59	3.499176910723013	44.78451966994492	21.282986420091042	30.433316999241022
60-61	3.470597034314286	44.60523588392667	21.452072379660866	30.472094702098186
62-63	3.4605464018874357	44.78953047710795	21.53929892041657	30.210624200588043
64-65	3.4715169975354248	44.73680212603633	21.361704362485398	30.429976513942847
66-67	3.4417250528456083	44.529476087351675	21.507213419244966	30.521585440557747
68-69	3.4381884703844836	44.78983690060287	21.564202689998098	30.20777193901455
70-71	3.4443746828469526	44.78844845355472	21.318566573584935	30.448610290013388
72-73	3.412817231947203	44.51232657346873	21.549147483480276	30.525708711103793
74-75	3.402986759407335	44.767210440351256	21.622434283909524	30.207368516331883
76-77	3.39095671078675	44.7531968052925	21.418311882356527	30.437534601564227
78-79	3.3658043441500807	44.55299530389386	21.57031502699817	30.510885324957894
80-81	3.3586687670987705	44.81546898593828	21.628282029730283	30.197580217232662
82-83	3.3578606526237174	44.81265202381428	21.41630126452218	30.413186059039827
84-85	3.323577860127686	44.61098322596797	21.58409351296457	30.48134540093977
86-87	3.3347377936093423	44.85588702778033	21.67690334634942	30.132471832260915
88-89	3.3761538364554955	44.849607439442764	21.48359770241686	30.290641021684884
90-91	3.3798598547224197	44.63297831060364	21.62438998430783	30.36277185036611
92-93	3.472182909113025	44.84588828604501	21.630314696850157	30.05161410799181
94-95	3.5315213831817913	44.88268706009276	21.41832400586591	30.167467550859534
96-97	3.6076938830949685	44.71444944285547	21.53122046051918	30.146636213530382
98-99	3.6790816457736706	44.95667786120487	21.573997464527515	29.790243028493947
100-101	3.793832971018791	45.02684933036145	21.230570839852046	29.948746858767716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4152.0
1	4111.0
2	3615.5
3	2944.5
4	2755.0
5	2812.5
6	2911.5
7	2940.0
8	2896.0
9	2991.5
10	3207.0
11	3409.5
12	3596.0
13	4438.5
14	5690.0
15	6644.0
16	7663.5
17	9409.5
18	12482.0
19	16621.0
20	23187.5
21	35428.0
22	57146.5
23	94593.5
24	154488.0
25	241770.0
26	363351.5
27	523446.0
28	714148.0
29	917527.5
30	1109495.0
31	1262625.0
32	1358236.0
33	1379979.5
34	1322735.0
35	1215101.5
36	1077576.0
37	925740.0
38	775296.5
39	632337.0
40	508312.0
41	407053.0
42	326033.5
43	262990.0
44	217114.5
45	183593.5
46	156963.5
47	136879.0
48	120137.5
49	104385.5
50	92911.0
51	84847.5
52	78339.0
53	74416.5
54	71837.5
55	68699.0
56	66253.5
57	64504.0
58	62672.0
59	60604.5
60	58005.5
61	54848.0
62	51127.0
63	47069.5
64	43125.0
65	39037.0
66	34793.0
67	30704.5
68	27135.5
69	23743.0
70	20169.5
71	17351.5
72	14980.5
73	12848.0
74	11290.0
75	9946.5
76	8885.0
77	8208.5
78	7690.0
79	7076.0
80	6598.5
81	6316.0
82	6044.5
83	5585.5
84	5162.5
85	5047.0
86	4947.0
87	4893.0
88	4007.5
89	3133.0
90	2955.5
91	2776.5
92	2494.0
93	2134.5
94	1957.0
95	1835.0
96	1774.5
97	1735.0
98	1720.5
99	1718.0
100	1719.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0063548155642067375
3	0.0
4	0.002847994785340409
5	3.0823057408258946E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.184217416631626E-5
12-13	0.0
14-15	0.0
16-17	2.8973981553525685E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.480617784343137E-5
30-31	0.012356701344806728
32-33	3.180736948124248E-6
34-35	0.001957204338263147
36-37	0.0011607423595096126
38-39	0.0
40-41	1.647676548299845E-4
42-43	4.31214201926263E-4
44-45	1.2037958742206397E-4
46-47	0.0
48-49	0.0025614826254502492
50-51	3.2861570437363187E-6
52-53	0.0
54-55	0.0
56-57	0.0
58-59	9.976023624820108E-5
60-61	0.006029662806115025
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0012212120875979501
74-75	9.191088864893717E-5
76-77	0.0
78-79	0.003348227404888323
80-81	2.406491476379083E-5
82-83	1.2417483236915026E-4
84-85	3.35789686818652E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.008217372113858477
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	12033.0
5-9	12147.0
10-14	6877.0
15-19	8351.0
20-24	20647.0
25-29	71707.0
30-34	134324.0
35-39	152984.0
40-44	135411.0
45-49	127560.0
50-54	114833.0
55-59	109059.0
60-64	104560.0
65-69	105161.0
70-74	119191.0
75-79	118692.0
80-84	125744.0
85-89	108225.0
90-94	141516.0
95-99	261662.0
100-102	1.3918539E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.34782726088542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.41941129798496	89.04123139142578
2	2.504944248987149	4.626523176072272
3	0.5402859245853774	1.4968269370529457
4	0.1891477590736867	0.6986953832688156
5	0.1297340246116176	0.5990327647346559
6	0.05632236727924968	0.31207489466569766
7	0.0251100125148574	0.16231985687685055
8	0.03211993786581107	0.23729651789298345
9	0.013527593332067172	0.112431946705672
>10	0.08256337171845873	1.5677973180320972
>50	0.004290930162780304	0.25350079171773976
>100	0.0021598146586807292	0.3681016283064118
>500	2.4440829632997965E-4	0.14727602082746705
>1k	1.2423559437789704E-4	0.26274503246703906
>5k	7.249967512708774E-6	0.03715159529936658
>10k+	6.823367086976159E-6	0.07699474465421988
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.714246572569886E-4	0.0	0.0
2	4.777103193537485E-4	0.0	0.0
3	4.839959814505083E-4	0.0	0.0
4	4.839959814505083E-4	0.0	0.0
5	5.091386298375477E-4	0.0	0.0
6	5.154242919343076E-4	0.0	6.285662096759848E-6
7	5.154242919343076E-4	0.0	6.285662096759848E-6
8	5.154242919343076E-4	0.0	6.285662096759848E-6
9	5.279956161278272E-4	0.0	6.285662096759848E-6
10-11	5.468526024181067E-4	0.0	6.285662096759848E-6
12-13	5.657095887083863E-4	0.0	6.285662096759848E-6
14-15	5.78280912901906E-4	6.285662096759848E-6	6.285662096759848E-6
16-17	5.845665749986659E-4	6.285662096759848E-6	6.285662096759848E-6
18-19	6.002807302405654E-4	6.285662096759848E-6	6.285662096759848E-6
20-21	6.568516891114042E-4	6.285662096759848E-6	6.285662096759848E-6
22-23	6.788515064500636E-4	6.285662096759848E-6	6.285662096759848E-6
24-25	7.039941548371029E-4	6.285662096759848E-6	6.285662096759848E-6
26-27	7.385652963692821E-4	6.285662096759848E-6	6.285662096759848E-6
28-29	8.485643830625795E-4	6.285662096759848E-6	6.285662096759848E-6
30-31	9.491349766107371E-4	6.285662096759848E-6	6.285662096759848E-6
32-33	0.0010434199080621349	1.2571324193519696E-5	6.285662096759848E-6
34-35	0.0010968480358845936	1.2571324193519696E-5	6.285662096759848E-6
36-37	0.0011439905016102925	1.571415524189962E-5	6.285662096759848E-6
38-39	0.0012697037435454893	1.8856986290279544E-5	6.285662096759848E-6
40-41	0.0013702743370936467	1.8856986290279544E-5	6.285662096759848E-6
42-43	0.0014959875790288438	1.8856986290279544E-5	6.285662096759848E-6
44-45	0.0016405578072543202	1.8856986290279544E-5	6.285662096759848E-6
46-47	0.0017662710491895173	1.8856986290279544E-5	6.285662096759848E-6
48-49	0.001948555249995553	1.8856986290279544E-5	6.285662096759848E-6
50-51	0.002118268126608069	1.8856986290279544E-5	6.285662096759848E-6
52-53	0.0022879810032205845	1.8856986290279544E-5	6.285662096759848E-6
54-55	0.0025079791766071794	1.8856986290279544E-5	6.285662096759848E-6
56-57	0.0026714063911229356	1.8856986290279544E-5	6.285662096759848E-6
58-59	0.002932261368138469	1.8856986290279544E-5	6.285662096759848E-6
60-61	0.0032088305003959024	1.8856986290279544E-5	6.285662096759848E-6
62-63	0.003519970774185515	1.8856986290279544E-5	6.285662096759848E-6
64-65	0.0038185397237816077	1.8856986290279544E-5	6.285662096759848E-6
66-67	0.004129679997571221	1.8856986290279544E-5	6.285662096759848E-6
68-69	0.004450248764505973	2.199981733865947E-5	6.285662096759848E-6
70-71	0.004792817348779383	2.5142648387039393E-5	6.285662096759848E-6
72-73	0.0052202423713590546	2.5142648387039393E-5	6.285662096759848E-6
74-75	0.0058079517774061	2.5142648387039393E-5	6.285662096759848E-6
76-77	0.006502517439098063	2.5142648387039393E-5	1.2571324193519696E-5
78-79	0.007131083648774047	3.142831048379924E-5	1.8856986290279544E-5
80-81	0.007954505383449589	3.142831048379924E-5	1.8856986290279544E-5
82-83	0.008862783556431386	3.142831048379924E-5	1.8856986290279544E-5
84-85	0.009947060268122459	3.142831048379924E-5	1.8856986290279544E-5
86-87	0.09495121163365428	3.142831048379924E-5	1.8856986290279544E-5
88-89	0.23215464388172824	3.142831048379924E-5	1.8856986290279544E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAATC	439900	0.0	75.9189	2
AATAAAT	446720	0.0	73.1714	1
TAAATCA	466670	0.0	72.894905	3
AATCATA	507545	0.0	68.5836	5
TCATAAA	514370	0.0	68.23748	7
AAATCAT	506225	0.0	67.38743	4
ATCATAA	529415	0.0	66.79169	6
CATAAAC	558975	0.0	62.69951	8
ATAAACA	624505	0.0	56.13782	9
AGTAAAT	3110	0.0	49.413876	1
GTAAATC	3180	0.0	48.90192	2
GATAAAT	5785	0.0	46.488335	1
GTCCCCA	1830	0.0	43.964214	1
GGCCCGT	1205	0.0	43.136127	1
AGAAATC	4565	0.0	43.076763	2
GTCCCCC	1080	0.0	41.43256	1
AGAGCAC	2555	0.0	40.692657	94-95
CGCTTGT	1855	0.0	40.2122	3
TGCCCGT	19495	0.0	39.970943	1
ATGAATC	1935	0.0	39.949352	2
>>END_MODULE