##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2212_lane2_GCCAAT_L002_R1_006.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253470
Sequences flagged as poor quality	0
Sequence length	2-101
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.585161952104787	30.0	16.0	31.0	16.0	33.0
2	25.469811023000748	30.0	16.0	31.0	16.0	34.0
3	28.826528840842737	31.0	28.0	31.0	19.0	34.0
4	32.47278496749826	35.0	32.0	37.0	25.0	37.0
5	29.70450720251594	35.0	26.0	35.0	16.0	37.0
6	32.80528298009398	35.0	32.0	37.0	28.0	37.0
7	34.18739125620828	35.0	33.0	37.0	30.0	37.0
8	34.74677695627579	35.0	35.0	37.0	32.0	37.0
9	36.47237906815709	38.0	35.0	39.0	32.0	39.0
10-11	36.63476694229693	39.0	35.0	39.0	32.0	39.0
12-13	36.54790249317919	38.5	35.0	39.0	32.0	39.0
14-15	37.79852708380919	40.0	36.5	41.0	32.5	41.0
16-17	37.80050211756523	40.0	37.0	41.0	32.5	41.0
18-19	37.71997856681575	40.0	36.5	41.0	32.5	41.0
20-21	37.83824376158218	40.0	37.0	41.0	32.5	41.0
22-23	37.67203590422888	39.5	37.0	40.5	32.0	41.0
24-25	37.59069914175318	39.5	37.0	40.0	32.0	41.0
26-27	37.37972137495112	39.5	36.5	40.0	31.5	41.0
28-29	37.28113200123347	39.5	36.0	40.0	30.5	41.0
30-31	37.42798371598657	40.0	36.0	40.0	31.5	41.0
32-33	37.179412109066234	39.0	36.0	40.0	30.5	41.0
34-35	37.064046703499315	39.5	36.0	40.0	30.0	41.0
36-37	37.101688821804714	39.5	36.0	40.0	30.0	41.0
38-39	37.01094980942748	39.0	36.0	40.0	30.0	41.0
40-41	37.15347487208166	39.0	36.0	40.0	31.0	41.0
42-43	37.18028480846705	39.5	36.0	40.0	31.0	41.0
44-45	37.04398014352306	39.0	36.0	40.0	30.5	41.0
46-47	37.010270070321454	39.0	35.5	40.0	30.5	41.0
48-49	37.079978123661505	39.0	36.0	40.0	31.0	41.0
50-51	37.027359781574845	39.0	35.5	40.0	31.0	41.0
52-53	36.94858573336691	39.0	35.0	40.0	31.0	41.0
54-55	36.95050615307462	39.0	35.0	40.0	31.0	41.0
56-57	36.806839979684305	39.0	35.0	40.0	31.0	41.0
58-59	36.70952435367265	39.0	35.0	40.0	31.0	41.0
60-61	36.34658207427496	38.0	35.0	40.0	30.0	41.0
62-63	36.334584331522414	38.0	34.5	40.0	30.0	41.0
64-65	36.123349575000645	37.0	34.0	40.0	30.0	41.0
66-67	35.61458536169925	37.0	34.0	39.0	29.5	40.0
68-69	35.29816753661894	36.0	34.0	39.0	30.0	40.0
70-71	35.00094301563702	36.0	34.0	38.5	30.0	39.5
72-73	34.620366825903226	35.0	33.5	37.0	30.0	39.0
74-75	34.23957903045979	35.0	33.0	37.0	29.0	39.0
76-77	32.33795589681236	33.5	30.5	35.0	27.0	36.5
78-79	33.38340283729502	34.0	32.5	36.0	29.0	37.0
80-81	33.29042251473439	34.0	33.0	35.0	29.0	36.5
82-83	33.26981624941722	34.0	33.0	35.0	29.0	36.0
84-85	33.27312486662055	34.0	33.0	35.0	29.0	36.0
86-87	33.02571395444482	34.0	33.0	35.0	29.0	36.0
88-89	33.040450093588376	34.0	33.0	35.0	29.0	35.0
90-91	32.893739794511745	34.0	32.5	35.0	29.0	35.0
92-93	32.90238130556283	34.0	32.5	35.0	29.0	35.0
94-95	32.906105094364136	34.0	32.5	35.0	29.0	35.0
96-97	33.02680184653353	34.0	33.0	35.0	29.0	35.0
98-99	33.106772858025565	34.0	33.0	35.0	29.5	35.0
100-101	32.800549736878935	34.0	32.5	35.0	28.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2316	1	0.0
2316	2	0.0
2316	3	0.0
2316	4	0.0
2316	5	0.0
2316	6	0.0
2316	7	0.0
2316	8	0.0
2316	9	0.0
2316	10-11	0.0
2316	12-13	0.0
2316	14-15	0.0
2316	16-17	0.0
2316	18-19	0.0
2316	20-21	0.0
2316	22-23	0.0
2316	24-25	0.0
2316	26-27	0.0
2316	28-29	0.0
2316	30-31	0.0
2316	32-33	0.0
2316	34-35	0.0
2316	36-37	0.0
2316	38-39	0.0
2316	40-41	0.0
2316	42-43	0.0
2316	44-45	0.0
2316	46-47	0.0
2316	48-49	0.0
2316	50-51	0.0
2316	52-53	0.0
2316	54-55	0.0
2316	56-57	0.0
2316	58-59	0.0
2316	60-61	0.0
2316	62-63	0.0
2316	64-65	0.0
2316	66-67	0.0
2316	68-69	0.0
2316	70-71	0.0
2316	72-73	0.0
2316	74-75	0.0
2316	76-77	0.0
2316	78-79	0.0
2316	80-81	0.0
2316	82-83	0.0
2316	84-85	0.0
2316	86-87	0.0
2316	88-89	0.0
2316	90-91	0.0
2316	92-93	0.0
2316	94-95	0.0
2316	96-97	0.0
2316	98-99	0.0
2316	100-101	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	211.0
16	93.0
17	112.0
18	74.0
19	77.0
20	56.0
21	68.0
22	84.0
23	145.0
24	244.0
25	545.0
26	1249.0
27	2495.0
28	4314.0
29	7005.0
30	9858.0
31	13050.0
32	16041.0
33	19842.0
34	24757.0
35	32123.0
36	40797.0
37	47993.0
38	30525.0
39	1707.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.885588038032115	44.32043239831144	12.727738982917112	33.06624058073934
2	10.326271353611867	47.085256637866415	17.916124196157337	24.672347812364382
3	6.833819932139193	18.814408585181095	45.02643415134538	29.32533733133433
4	9.63527791262615	42.89401707377719	11.573937033046402	35.89676798055026
5	11.47311020666219	43.9815070928992	14.674991109179278	29.870391591259338
6	8.89586190271065	49.65716747220784	17.5917546307091	23.855215994372408
7	6.784821739267977	28.89619119926608	41.44087501186296	22.878112049602986
8	7.022457464525751	31.608574740198346	15.744355964856066	45.62461183041984
9	6.570382827183698	58.29379760609358	17.38213473142744	17.75368483529528
10-11	6.477078200294871	45.32569438260061	29.564520438151217	18.632706978953305
12-13	5.437282981815014	58.98361252875429	16.083794259831887	19.495310229598807
14-15	6.1677323791097916	46.12214668612607	15.427252006503188	32.282868928260946
16-17	5.981620917052453	57.12035942022096	16.231753144233608	20.666266518492986
18-19	19.779525124941046	29.76331548555598	17.394052766537595	33.06310662296537
20-21	31.19740048096653	30.85560893019641	17.265826197806906	20.68116439103015
22-23	3.8710035766216304	45.92893968939134	16.81151025476734	33.38854647921969
24-25	3.67843163733202	45.40022281489484	16.295672585228683	34.625672962544456
26-27	3.727403042127897	45.339253006291315	29.745560245281517	21.187783706299275
28-29	3.7553322362639996	44.00932242654695	16.31784122317905	35.91750411401
30-31	3.7031173281761975	32.23784282092648	29.997695321502647	34.061344529394674
32-33	3.760728532860539	46.074263609622435	21.203006003948907	28.96200185356812
34-35	3.7558875911283485	45.58750147063	20.77178662274385	29.884824315497806
36-37	3.7742135266668164	45.36435008513187	21.03544061106898	29.825995777132334
38-39	3.7587236232629953	45.442099845599586	21.30972722593927	29.489449305198146
40-41	3.8265803007886485	45.32530180398592	21.05707027380967	29.791047621415757
42-43	3.828936367180903	44.90514187539485	21.316024861815098	29.94989689560915
44-45	3.8033066210060036	45.212869760092666	21.243539624684455	29.740283994216878
46-47	3.8213440370943594	45.29637692328807	21.073346241472287	29.808932798145282
48-49	3.8253153426769773	44.89048226881065	21.387231674655986	29.896970713856387
50-51	3.8270901003060223	45.11827653881226	21.426451148596847	29.62818221228487
52-53	3.8241180563310113	45.148558225032	21.15977632517029	29.867547393466705
54-55	3.810814426716269	44.90870013680953	21.325239848257944	29.955245588216258
56-57	3.793119145735288	45.01277885321986	21.3410100388243	29.853091962220553
58-59	3.8273895865731005	44.84207362492322	21.32104073285156	30.009496055652114
60-61	3.789802225510195	44.93591371113893	21.396021747314418	29.87826231603646
62-63	3.7862067146198624	45.09241835352878	21.527716712685322	29.593658219166034
64-65	3.8275875008001274	44.99848803561614	21.345405504433256	29.828518959150475
66-67	3.7362003990246064	44.76258035912215	21.584571048547993	29.916648193305257
68-69	3.7845829121561745	45.01044222488411	21.45509843299832	29.749876429961393
70-71	3.7651104091072702	44.799917726938396	21.33420972029395	30.100762143660393
72-73	3.754675383572285	44.56914628506257	21.596118701983304	30.08005962938184
74-75	3.723920372234082	45.00882295864718	21.603160920837077	29.664095748281667
76-77	3.744816833065144	44.89399898776466	21.44123247595941	29.919951703210785
78-79	3.7147671743279513	44.83054267843583	21.504650584771987	29.950039562464227
80-81	3.6922646882940433	45.0082826787764	21.60942873574016	29.690023897189388
82-83	3.6757884171341884	45.0259333353285	21.366397856269696	29.93188039126761
84-85	3.7003789684100443	44.75922318416754	21.571353875056104	29.96904397236631
86-87	3.7027221796034127	44.952925587558404	21.677011414092103	29.66734081874608
88-89	3.736663116392922	45.07866659192406	21.442719135975487	29.74195115570753
90-91	3.7089570114175947	44.849871076522774	21.699770333040576	29.741401579019055
92-93	3.831974133879375	45.022684814518286	21.614223532218105	29.531117519384235
94-95	3.89434755120764	45.000213909844135	21.511486958629835	29.593951580318397
96-97	3.983953056703191	44.76522363930304	21.64181785521825	29.609005448775523
98-99	4.042913471540192	45.12615205979825	21.675899271874016	29.155035196787537
100-101	4.292013718765311	45.13694267515923	21.282949534541892	29.28809407153356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	78.0
2	77.0
3	74.0
4	73.5
5	78.5
6	88.0
7	89.5
8	86.5
9	96.0
10	107.0
11	112.5
12	120.5
13	148.5
14	180.5
15	187.5
16	198.0
17	233.0
18	298.5
19	378.5
20	521.0
21	770.0
22	1163.5
23	1881.5
24	3003.5
25	4535.0
26	6683.0
27	9485.5
28	12881.0
29	16560.0
30	19866.5
31	22431.5
32	24026.0
33	23995.5
34	22520.5
35	20291.5
36	17523.5
37	14765.0
38	12169.5
39	9822.5
40	7916.0
41	6369.0
42	5090.0
43	4168.5
44	3571.0
45	3042.0
46	2639.5
47	2363.0
48	2071.5
49	1817.5
50	1664.5
51	1535.5
52	1421.0
53	1344.0
54	1266.0
55	1188.5
56	1180.0
57	1183.5
58	1164.0
59	1133.5
60	1086.0
61	1030.0
62	984.0
63	920.0
64	852.5
65	800.5
66	712.0
67	643.0
68	581.5
69	517.5
70	438.0
71	376.0
72	347.5
73	319.0
74	297.5
75	268.5
76	245.5
77	241.0
78	234.5
79	215.5
80	197.5
81	193.5
82	190.5
83	174.0
84	160.0
85	156.5
86	152.5
87	150.0
88	118.0
89	86.5
90	83.0
91	80.0
92	65.5
93	45.5
94	41.0
95	41.5
96	41.0
97	41.0
98	41.0
99	41.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.014223289846941597
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.981826941837454E-4
28-29	0.0013979450208193955
30-31	0.015829633073112866
32-33	6.044207332429388E-4
34-35	0.0064906352303567005
36-37	0.005722823232209685
38-39	0.0
40-41	0.0010360482632722962
42-43	0.0
44-45	0.0014688416714578884
46-47	0.0
48-49	0.00849478738609021
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	8.754880846071685E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.002280205309686084
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.014876946400487397
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
2-3	99.0
4-5	332.0
6-7	250.0
8-9	116.0
10-11	81.0
12-13	82.0
14-15	85.0
16-17	79.0
18-19	112.0
20-21	140.0
22-23	202.0
24-25	598.0
26-27	744.0
28-29	801.0
30-31	1206.0
32-33	1537.0
34-35	1919.0
36-37	1795.0
38-39	1600.0
40-41	1511.0
42-43	1535.0
44-45	1508.0
46-47	1368.0
48-49	1307.0
50-51	1318.0
52-53	1153.0
54-55	1083.0
56-57	1072.0
58-59	1138.0
60-61	1000.0
62-63	956.0
64-65	953.0
66-67	976.0
68-69	930.0
70-71	940.0
72-73	1047.0
74-75	1360.0
76-77	985.0
78-79	1075.0
80-81	982.0
82-83	1094.0
84-85	1098.0
86-87	960.0
88-89	1150.0
90-91	1093.0
92-93	1386.0
94-95	1542.0
96-97	1910.0
98-99	2355.0
100-101	204907.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.57662167587702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.1069933202906	96.70525592647665
2	0.5948280787348549	1.1608262880179945
3	0.16642307128873768	0.48717003195835995
4	0.043382483665688656	0.16932464784027268
5	0.016272507102602297	0.07939081346343228
6	0.013774559716843143	0.08064450013453074
7	0.009993475308434788	0.06825906915788518
8	0.010657002605748252	0.08318994491679461
9	0.008016650264352816	0.07040138849573169
>10	0.02348127069295488	0.4742826011630693
>50	0.004541795535273238	0.31283281461045437
>100	0.0016357847938956792	0.3084219737648293
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATAAATCATAAACAAACGGGCAACATACCT	362	0.14281769045646428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	7.890480135716258E-4	0.0	0.0
2	7.890480135716258E-4	0.0	0.0
3	7.890480135716258E-4	0.0	0.0
4	7.890480135716258E-4	0.0	0.0
5	7.890480135716258E-4	0.0	0.0
6	7.890480135716258E-4	0.0	0.0
7	7.890480135716258E-4	0.0	0.0
8	7.890480135716258E-4	0.0	0.0
9	7.890480135716258E-4	0.0	0.0
10-11	7.890480135716258E-4	0.0	0.0
12-13	7.890480135716258E-4	0.0	0.0
14-15	7.890480135716258E-4	0.0	0.0
16-17	7.890480135716258E-4	0.0	0.0
18-19	7.890480135716258E-4	0.0	0.0
20-21	7.890480135716258E-4	0.0	0.0
22-23	7.890480135716258E-4	0.0	0.0
24-25	7.890480135716258E-4	0.0	0.0
26-27	7.890480135716258E-4	0.0	0.0
28-29	7.890480135716258E-4	0.0	0.0
30-31	9.863100169645322E-4	0.0	0.0
32-33	0.0011835720203574387	0.0	0.0
34-35	0.0011835720203574387	0.0	0.0
36-37	0.0011835720203574387	0.0	0.0
38-39	0.001380834023750345	0.0	0.0
40-41	0.0023671440407148775	0.0	0.0
42-43	0.002958930050893597	0.0	0.0
44-45	0.003156192054286503	0.0	0.0
46-47	0.0035507160610723162	0.0	0.0
48-49	0.0035507160610723162	0.0	0.0
50-51	0.0035507160610723162	0.0	0.0
52-53	0.0035507160610723162	0.0	0.0
54-55	0.003945240067858129	0.0	0.0
56-57	0.004142502071251036	0.0	0.0
58-59	0.004734288081429755	0.0	0.0
60-61	0.004734288081429755	0.0	0.0
62-63	0.004734288081429755	0.0	0.0
64-65	0.005128812088215568	0.0	0.0
66-67	0.005523336095001381	0.0	0.0
68-69	0.005720598098394288	0.0	0.0
70-71	0.00670690811535882	0.0	0.0
72-73	0.007298694125537538	0.0	0.0
74-75	0.008482266145894979	0.0	0.0
76-77	0.00946857616285951	0.0	0.0
78-79	0.010257624176431135	0.0	0.0
80-81	0.010849410186609856	0.0	0.0
82-83	0.0122302442103602	0.0	0.0
84-85	0.013019292223931826	0.0	0.0
86-87	0.09330492760484475	0.0	0.0
88-89	0.2286266619323786	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTG	20	2.6541427E-5	87.26463	2
CGCTTGT	60	0.0	79.99258	3
CGGTTGT	45	5.4569682E-11	77.568565	3
CCCGGTT	45	5.4569682E-11	77.568565	1
ATAAATC	7040	0.0	75.61282	2
TAAATCA	7580	0.0	73.39209	3
CGTCCAC	30	2.2600998E-6	72.72053	1
CCGCTTG	55	3.6379788E-12	71.39834	2
AATAAAT	7290	0.0	70.087036	1
CGATTGT	25	8.022989E-5	69.81171	3
GTCCGTT	50	1.382432E-10	69.81171	1
CCGGTTG	50	1.382432E-10	69.81171	2
GTCCCCA	50	1.382432E-10	69.81171	1
TCATAAA	8780	0.0	68.63357	7
AATCATA	8285	0.0	68.33254	5
AAATCAT	8255	0.0	67.35139	4
ATCATAA	9005	0.0	67.08609	6
AACGTGC	20	0.0029018782	65.44847	1
CGTGCAA	20	0.0029018782	65.44847	3
GCTGCCC	20	0.0029018782	65.44847	1
>>END_MODULE