FastQCFastQC Report
Thu 21 May 2015
20150521_trimmed_2212_lane2_1000ppm_CTTGTA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150521_trimmed_2212_lane2_1000ppm_CTTGTA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57867827
Sequences flagged as poor quality0
Sequence length1-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC49050.029.78896945
CTAGATC54800.028.17927745
TAAGATC53450.024.82249645
ATAGATC64700.018.8824845
CAAGATC174500.016.83070845
CCTAGAT34800.015.58716245
ACTAGAT43900.015.58428345
ACAGATC110850.014.68019645
CTTAGAT29850.014.21485744
CTAAGAT29350.014.12657244
TCTAGAT32800.012.738420545
TCAGATC100400.012.65502445
ACAAGAT125400.012.29490545
ATAAGAT65150.011.81379645
AAAGATC87150.011.41091145
ATTAGAT73600.011.08822345
CCTTAGA25750.010.62757445
CCAAGAT115000.010.07102945
ACTTAGA21650.09.25442145
CCTAAGA21850.09.07305443