FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 106A_Female_Mix_GATCAG_L004_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 39823239 |
| Filtered Sequences | 5779631 |
| Sequence length | 76 |
| %GC | 41 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
![[WARN]](Icons/warning.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 157748 | 0.39612046624334096 | Illumina Single End PCR Primer 1 (100% over 50bp) |
| CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC | 64705 | 0.16248050541544348 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 11572055 | 8.989903 | 45.890198 | 41 |
| AAAAA | 26929590 | 4.10783 | 18.377224 | 61 |
| GGGGA | 4336655 | 2.4328036 | 21.741423 | 21 |
| CGGGG | 2759630 | 2.2310333 | 21.333677 | 40 |
| GGGGC | 2636780 | 2.131715 | 15.283671 | 45 |
| GGAGG | 3495490 | 1.9609216 | 7.126769 | 18 |
| GGAAA | 6686885 | 1.9561011 | 13.936063 | 23 |
| AGGGG | 3465160 | 1.943907 | 19.143902 | 28 |
| GGGAA | 4666390 | 1.890344 | 23.790419 | 22 |
| GAGGG | 3318215 | 1.8614728 | 10.19948 | 27 |
| GGTGG | 3114515 | 1.8244195 | 10.978796 | 41 |
| GAAGA | 6191915 | 1.8113086 | 8.290127 | 7 |
| TGGGG | 3084880 | 1.8070599 | 9.479804 | 20 |
| GGAGA | 4460035 | 1.80675 | 6.7958937 | 6 |
| GGGAG | 3178000 | 1.7828141 | 10.075573 | 30 |
| CTGGG | 2825975 | 1.7227142 | 5.638174 | 1 |
| GAAAA | 8070985 | 1.7049105 | 6.128937 | 24 |
| GGAAG | 4192285 | 1.698285 | 10.376615 | 6 |
| CTCCA | 3536365 | 1.6858966 | 7.3128605 | 1 |
| AAAAG | 7879095 | 1.6643758 | 5.5527344 | 25 |
| AAAGG | 5686595 | 1.6634883 | 8.912706 | 26 |
| CTCTG | 3477530 | 1.6634747 | 5.0019693 | 1 |
| CTCCT | 3268700 | 1.627163 | 6.426093 | 1 |
| GGGCG | 1970650 | 1.5931796 | 13.6699705 | 12 |
| CTGGA | 3580765 | 1.5762619 | 6.192334 | 1 |
| GGCGG | 1860485 | 1.5041163 | 16.339285 | 13 |
| TAAAA | 9382690 | 1.4944869 | 10.495988 | 58 |
| GAAAG | 5107440 | 1.4940692 | 7.2510195 | 24 |
| CTGGT | 3242490 | 1.4904358 | 5.2353754 | 1 |
| TTAAA | 8796855 | 1.4631009 | 10.448849 | 57 |
| GTGGG | 2475295 | 1.4499774 | 7.042526 | 19 |
| CCCCG | 1584400 | 1.4436171 | 5.838868 | 70 |
| CCCTG | 2186680 | 1.4436153 | 6.1298156 | 1 |
| GGGGT | 2406755 | 1.4098281 | 5.043109 | 42 |
| AGAGG | 3477890 | 1.4088854 | 6.676052 | 26 |
| GCCCC | 1503195 | 1.3696277 | 6.833769 | 47 |
| GAGAG | 3349720 | 1.3569639 | 5.74438 | 7 |
| TTTAA | 7699940 | 1.3372613 | 5.9736776 | 56 |
| GGGCC | 1586530 | 1.3347938 | 12.352526 | 45 |
| TCATT | 5212795 | 1.3046759 | 5.6546783 | 54 |
| GGCCG | 1523695 | 1.2819289 | 7.8906255 | 47 |
| AAGAG | 4324635 | 1.2650766 | 6.1827235 | 26 |
| GGGTG | 2146015 | 1.2570919 | 5.274448 | 17 |
| ATCAT | 5220980 | 1.2514167 | 5.5166125 | 53 |
| GTGGT | 2832755 | 1.2512181 | 7.7008414 | 42 |
| CCGGG | 1482525 | 1.2472913 | 11.723874 | 39 |
| TGGTC | 2672175 | 1.2282861 | 7.846746 | 43 |
| AAGGG | 3024100 | 1.2250559 | 10.998267 | 27 |
| CTCGG | 1918075 | 1.2168055 | 11.411549 | 38 |
| CGCCG | 1383985 | 1.2117357 | 15.332676 | 47 |
| GCCGG | 1404620 | 1.1817476 | 6.850792 | 47 |
| TGTAG | 3605575 | 1.1500219 | 5.7330284 | 18 |
| GGCCC | 1307835 | 1.1450634 | 9.460259 | 46 |
| AGGGA | 2826575 | 1.1450391 | 8.670657 | 21 |
| GTGTA | 3588050 | 1.1444323 | 5.6860056 | 17 |
| GCGCC | 1306965 | 1.1443014 | 11.458019 | 45 |
| GCGGG | 1409125 | 1.1392125 | 16.107363 | 14 |
| GGCGC | 1345865 | 1.1323154 | 11.079603 | 44 |
| TCGGG | 1835050 | 1.1186464 | 9.43284 | 38 |
| CGCCC | 1214165 | 1.1062796 | 5.988932 | 46 |
| ATCTC | 3155600 | 1.090021 | 6.4413033 | 36 |
| CCCGG | 1243010 | 1.0883062 | 5.3814406 | 71 |
| AGATC | 3331740 | 1.0590862 | 5.8182917 | 34 |
| GATCT | 3034590 | 1.0072616 | 6.10962 | 35 |
| TATCA | 4130580 | 0.9900587 | 5.0645766 | 52 |
| CCGGC | 1125590 | 0.9855003 | 5.2969213 | 72 |
| CGGAA | 2316470 | 0.97655594 | 11.332441 | 5 |
| TCGCC | 1464505 | 0.9668456 | 10.735161 | 46 |
| GCCGT | 1515740 | 0.9615686 | 11.200226 | 48 |
| AGAGC | 2241225 | 0.94483495 | 7.2560234 | 9 |
| CGGCG | 1106885 | 0.93125445 | 7.8515835 | 12 |
| GTAGA | 3039190 | 0.9283407 | 5.886695 | 32 |
| AGTGT | 2885730 | 0.9204226 | 5.0575023 | 29 |
| GGTCG | 1509035 | 0.9199077 | 10.573482 | 44 |
| GTCGT | 1974340 | 0.9075208 | 7.6669545 | 14 |
| GTATC | 2698050 | 0.895555 | 6.6538825 | 51 |
| CGGTG | 1467080 | 0.8943319 | 10.172415 | 40 |
| GTAGG | 2056010 | 0.86969525 | 7.3197556 | 19 |
| TCTCG | 1815415 | 0.86840284 | 8.385261 | 37 |
| GAGTG | 2036570 | 0.86147213 | 6.4151316 | 28 |
| TCGGA | 1956065 | 0.8610648 | 9.335511 | 4 |
| CATTA | 3570260 | 0.8557557 | 5.2293987 | 55 |
| CGTGT | 1808935 | 0.8314911 | 7.6661224 | 16 |
| TCCGG | 1310655 | 0.83146495 | 7.3825197 | 38 |
| GCGGC | 973945 | 0.8194082 | 6.4223595 | 11 |
| TCGTG | 1771435 | 0.8142539 | 7.6844487 | 15 |
| CGGGA | 1384815 | 0.8084526 | 7.3235364 | 4 |
| TCGGT | 1687195 | 0.77553236 | 7.526276 | 39 |
| GATCG | 1761325 | 0.77533966 | 8.368295 | 2 |
| ACGGC | 1275575 | 0.7749604 | 5.0779653 | 11 |
| GCCCG | 853945 | 0.7476639 | 8.467608 | 47 |
| ATCGG | 1688290 | 0.7431895 | 8.534322 | 3 |
| TAGGG | 1719620 | 0.7274018 | 7.3465586 | 20 |
| GAGCG | 1201325 | 0.7013315 | 9.649945 | 10 |
| CGTCG | 1102240 | 0.6992488 | 10.225662 | 13 |
| GCGTC | 1076490 | 0.6829133 | 10.204469 | 12 |
| GTCGC | 1035105 | 0.6566591 | 10.322859 | 45 |
| AGCGT | 1423380 | 0.62657547 | 7.2503195 | 11 |
| CCGTA | 1260555 | 0.57746416 | 7.5787153 | 49 |
| CGTAT | 1673465 | 0.5554678 | 5.4815407 | 50 |