FastQCFastQC Report
Wed 19 Dec 2012
106A_Male_Mix_TAGCTT_L004_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 106A_Male_Mix_TAGCTT_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 59446949
Filtered Sequences 7507358
Sequence length 76
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC 397072 0.667943446517331 TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG 198477 0.33387247510381063 TruSeq Adapter, Index 10 (100% over 49bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC 72672 0.12224681202730858 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 21362380 2.4161315 6.894606 65
GAAGA 9506745 2.0603387 9.571571 6
TCTTC 9080420 1.9828024 8.983986 53
CTCCA 6444030 1.8793584 12.157717 24
TCCAG 6302610 1.862511 12.460335 25
CTTCT 8440270 1.8430191 9.197941 54
TTCTG 8287640 1.8337102 9.054817 55
GAAAA 11506845 1.801545 5.8232837 63
GGAAG 5979605 1.7938954 12.274188 5
CTGGG 4251190 1.7621106 6.184793 1
GTCTG 5705775 1.727929 12.583661 17
CTGGA 5661185 1.6951655 6.5364065 1
GGAAA 7770335 1.6840172 5.0227623 1
CCAGT 5594235 1.6531762 11.924765 26
CTGCT 5504125 1.6450275 11.499886 57
TGAAA 10176480 1.6113614 5.775335 62
CTGAA 7254075 1.5691661 9.302034 19
CTGGT 5135925 1.5553564 5.2372303 1
TCTGA 6958750 1.5223854 9.351503 18
TCTGC 4969220 1.4851595 11.250182 56
CCCTG 3513075 1.4182652 5.077168 1
CACAC 4856120 1.4003447 12.018367 12
CGGGG 2454080 1.3922678 5.042073 1
TTGAA 8625625 1.3813139 5.722629 61
AAGAG 6293370 1.3639234 9.122475 7
CAGTC 4547335 1.3438023 11.752366 27
GAACT 6032285 1.3048744 8.966244 21
ATGCC 4393390 1.2983092 11.264564 47
TGAAC 5990710 1.2958812 9.02878 20
CTTGA 5913130 1.2936321 7.4819136 60
TGCTT 5773460 1.2774267 8.64757 58
GCTTG 4141680 1.2542607 10.917259 59
TCACT 5755830 1.2427249 8.68399 30
AGCAC 4216320 1.2319852 12.827653 10
GTCAC 4166950 1.2313931 11.671939 29
CGTCT 3998405 1.1950104 11.7097435 16
GCACA 4084030 1.1933308 12.229305 11
GTCTT 5374160 1.1890782 8.130512 52
AGCTT 5406480 1.1827909 8.838884 35
ACTCC 4001775 1.1670911 11.630546 23
AACTC 5445305 1.1624727 8.694943 22
GAGCA 3829645 1.1338532 12.569927 9
CCGTC 2807105 1.1332575 13.860269 50
ATCTC 5229505 1.1290876 8.577457 40
AGAGC 3781090 1.1194774 12.40664 8
AGTCA 5169765 1.1182983 8.698747 28
CTCGT 3560320 1.0640792 10.918139 42
CGGAA 3497615 1.0355481 11.632756 4
GCCGT 2474970 1.0124329 13.853193 49
TGCCG 2450285 1.0023351 14.762306 48
TCGGA 3183840 0.953358 11.801563 3
TCTCG 3176725 0.94943345 11.13353 41
GTATG 4282765 0.9493883 8.264248 45
CACGT 3068280 0.9067204 11.706487 14
CACTA 4246600 0.9065711 8.376495 31
ACGTC 3027840 0.89476985 11.540093 15
ACACG 2952715 0.86276686 11.479111 13
GATCG 2857765 0.8557192 11.560196 1
TTATC 5303210 0.84765834 6.1837687 38
TATCT 5254870 0.8399317 6.236254 39
CTAGC 2824750 0.8347539 11.153915 33
ACTAG 3707305 0.8019461 8.29643 32
ATCGG 2629285 0.787304 11.448625 2
TATGC 3467910 0.75868446 7.9531713 46
TAGCT 3412105 0.74647576 8.277485 34
TCGTA 3389040 0.74142987 8.022346 43
GCTTA 2893310 0.6329776 8.203044 36
CGTAT 2871415 0.6281876 7.9408565 44
CTTAT 3761335 0.60120696 6.0646515 37
Produced by FastQC (version 0.10.1)