FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 106A_Male_Mix_TAGCTT_L004_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 59446949 |
| Filtered Sequences | 7507358 |
| Sequence length | 76 |
| %GC | 42 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
![[WARN]](Icons/warning.png)
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 190866 | 0.3210694631275358 | Illumina Single End PCR Primer 1 (100% over 50bp) |
| CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC | 79317 | 0.13342484573934987 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 7053450 | 3.863451 | 13.636012 | 41 |
| AAAAA | 25622465 | 2.8641813 | 7.889504 | 61 |
| GAAGA | 9115190 | 1.9240794 | 5.228443 | 7 |
| GGTGG | 4474660 | 1.8197528 | 8.853806 | 41 |
| GGAAA | 8574850 | 1.8100219 | 7.624768 | 23 |
| CTGGG | 4290520 | 1.7466462 | 6.2611623 | 1 |
| GGGGA | 4274455 | 1.7037886 | 6.951362 | 21 |
| CTCCA | 5683050 | 1.6870302 | 6.5282483 | 1 |
| CTGGA | 5691575 | 1.6861206 | 6.662705 | 1 |
| GGAAG | 5729770 | 1.6620086 | 6.9499807 | 6 |
| CTCCT | 5410535 | 1.6386983 | 5.436374 | 1 |
| CGGGG | 2859485 | 1.5678489 | 6.6598377 | 40 |
| CTGGT | 5126600 | 1.5495408 | 5.262696 | 1 |
| GGGGC | 2795380 | 1.5327004 | 5.345776 | 42 |
| GGGAA | 5278690 | 1.5311662 | 10.855153 | 22 |
| GAAAG | 6876605 | 1.4515479 | 5.491901 | 24 |
| CCCTG | 3554460 | 1.4499527 | 5.406536 | 1 |
| CTGGC | 3393245 | 1.3827791 | 5.076286 | 1 |
| TAAAA | 11835730 | 1.3498706 | 5.091903 | 58 |
| AGGGG | 3321090 | 1.3237793 | 5.7635236 | 28 |
| AAGAG | 6179570 | 1.3044144 | 5.1718597 | 26 |
| GTGGT | 4255615 | 1.2849711 | 6.334232 | 42 |
| TGGTC | 3989340 | 1.2057981 | 6.2216454 | 43 |
| AGGGA | 4156345 | 1.2056125 | 6.55517 | 21 |
| CTCGG | 2670705 | 1.0883373 | 8.247234 | 38 |
| CGCCG | 1865220 | 1.0247824 | 10.698289 | 47 |
| AGAGC | 3282825 | 0.9532053 | 5.962976 | 9 |
| GAGTG | 3140855 | 0.92952585 | 5.5609155 | 28 |
| CGGAA | 3098845 | 0.8997846 | 6.535628 | 5 |
| CGGTG | 2200100 | 0.895648 | 7.8529134 | 40 |
| GTCGT | 2872790 | 0.86831534 | 6.0478992 | 14 |
| TCGCC | 2117345 | 0.8637178 | 7.8383565 | 46 |
| GTATC | 3876695 | 0.85270023 | 5.2482224 | 51 |
| GCCGT | 2091435 | 0.85227937 | 8.124679 | 48 |
| GGTCG | 2042220 | 0.83137596 | 8.150814 | 44 |
| GTAGG | 2790150 | 0.82573575 | 5.9057145 | 19 |
| TCTCG | 2692035 | 0.8145109 | 6.178571 | 37 |
| TCGGA | 2722590 | 0.80656326 | 6.1686935 | 4 |
| CGTGT | 2566635 | 0.7757784 | 6.0004225 | 16 |
| TCGGT | 2428870 | 0.73413825 | 5.77824 | 39 |
| TCGTG | 2403925 | 0.7265985 | 5.990828 | 15 |
| GATCG | 2437695 | 0.7221635 | 6.0123053 | 2 |
| CGTCG | 1684615 | 0.68649644 | 7.816735 | 13 |
| TAGGG | 2273145 | 0.6727298 | 5.8248463 | 20 |
| ATCGG | 2216480 | 0.65662897 | 5.8999214 | 3 |
| GAGCG | 1605095 | 0.6404398 | 7.662486 | 10 |
| GCGTC | 1513900 | 0.6169284 | 7.7582293 | 12 |
| AGCGT | 2048215 | 0.60678065 | 5.8264623 | 11 |
| GTCGC | 1483660 | 0.6046054 | 7.65814 | 45 |
| CCGTA | 1714765 | 0.5085145 | 5.64516 | 49 |