FastQCFastQC Report
Wed 19 Dec 2012
108A_Male_Mix_AGTCAA_L004_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 108A_Male_Mix_AGTCAA_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 55791565
Filtered Sequences 6920427
Sequence length 76
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 89944 0.16121433410229666 Illumina Single End PCR Primer 1 (100% over 50bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC 67512 0.1210075394013414 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 5775810 3.2467446 10.323603 41
AAAAA 20862670 2.583708 5.988569 61
GGAAA 8108100 1.8389813 5.240808 23
CTCCA 5806910 1.8013119 6.9230113 1
CTGGA 5736330 1.7885083 7.0781736 1
CTGGG 4201495 1.7727726 6.583373 1
GGTGG 4167695 1.762997 5.094448 41
CAGAA 7708490 1.7438977 5.0369143 1
CTCCT 5412675 1.7098916 5.73482 1
GGGGA 3900165 1.6200407 5.5544543 21
CTGGT 5019375 1.5937464 5.3880596 1
GGGAA 4742345 1.4556055 7.0966554 22
CCCTG 3420180 1.435771 5.267261 1
CTGGC 3366950 1.417031 5.229928 1
CGCCG 1484070 0.82788515 5.2506213 47
Produced by FastQC (version 0.10.1)