{ "metadata": { "name": "IntersectBed_IDTargetLoci", "signature": "sha256:aa3e2c1e465974cecd5bf14401def60e6c6b089d0387b00d08b61f58bbf50d91" }, "nbformat": 3, "nbformat_minor": 0, "worksheets": [ { "cells": [ { "cell_type": "heading", "level": 3, "metadata": {}, "source": "Using the intersectBed function to classify our target loci with respect to oyster genomic regions" }, { "cell_type": "code", "collapsed": false, "input": "cd /Users/Shared/Apps/bedtools-2.17.0 ", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": "/Users/Shared/Apps/bedtools-2.17.0\n" } ], "prompt_number": 5 }, { "cell_type": "code", "collapsed": false, "input": "cd bin", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": "/Users/Shared/Apps/bedtools-2.17.0/bin\n" } ], "prompt_number": 7 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_exon.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_exon.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 8 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_exon.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 45 180 1685 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_exon.txt\r\n" } ], "prompt_number": 9 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_gene.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_gene.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 10 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_gene.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 197 788 7398 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_gene.txt\r\n" } ], "prompt_number": 11 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_intron.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_intron.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 12 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_intron.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 152 608 5713 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_intron.txt\r\n" } ], "prompt_number": 13 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_TE.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_TE.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 59 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_TE.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 80 320 2988 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_TE.txt\r\n" } ], "prompt_number": 60 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_1k5p_gene_promoter.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_promoter.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 16 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_promoter.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 24 96 910 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_promoter.txt\r\n" } ], "prompt_number": 17 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_COMP_gene_prom_TE.bed > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_COMP.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 19 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_COMP.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 214 856 7808 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_COMP.txt\r\n" } ], "prompt_number": 20 }, { "cell_type": "raw", "metadata": {}, "source": "Lineage specific counts\n45 exon\n197 gene\n152 intron\n80 TE\n24 promoter\n214 complement (other)" }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_exon.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_exon.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 21 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_exon.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 30 120 1061 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_exon.txt\r\n" } ], "prompt_number": 22 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_gene.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_gene.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 23 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_gene.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 167 668 5976 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_gene.txt\r\n" } ], "prompt_number": 24 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_intron.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_intron.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 25 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_intron.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 137 548 4915 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_intron.txt\r\n" } ], "prompt_number": 26 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_TE.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_TE.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 27 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_TE.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 57 228 1983 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_TE.txt\r\n" } ], "prompt_number": 28 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_1k5p_gene_promoter.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_1k5p_gene_promoter.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 29 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_promoter.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 18 72 634 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_1k5p_gene_promoter.txt\r\n" } ], "prompt_number": 30 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_COMP_gene_prom_TE.bed > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_COMP.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 32 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_COMP.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 176 704 6052 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_COMP.txt\r\n" } ], "prompt_number": 33 }, { "cell_type": "raw", "metadata": {}, "source": "Developmentally different counts\n30 exon\n167 gene\n137 intron\n57 TE\n18 promoter\n176 complement (other)" }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_exon.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_exon.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 34 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_exon.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 1129658 11296580 110957829 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_exon.txt\r\n" } ], "prompt_number": 35 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_gene.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_gene.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 36 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_gene.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 3938356 39383560 387141916 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_gene.txt\r\n" } ], "prompt_number": 37 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_intron.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_intron.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 38 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_intron.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 2815997 28159970 276901690 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_intron.txt\r\n" } ], "prompt_number": 39 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_TE.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_TE.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 72 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_TE.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 589509 5895090 57596188 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_TE.txt\r\n" } ], "prompt_number": 73 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_1k5p_gene_promoter.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_promoter.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 42 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_promoter.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 593081 5930810 58106466 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_promoter.txt\r\n" } ], "prompt_number": 43 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_COMP_gene_prom_TE.bed > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_COMP.txt -u", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 46 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_COMP.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 5121236 51212360 499097421 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_COMP.txt\r\n" } ], "prompt_number": 47 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Lineage_specific_loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_TE.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_TE_names.txt -wb", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 57 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_TE_names.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 112 1456 10347 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/lineage_TE_names.txt\r\n" } ], "prompt_number": 58 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Developmentally_Different_Loci.bed -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_TE.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_TE_names.txt -wb", "language": "python", "metadata": {}, "outputs": [], "prompt_number": 69 }, { "cell_type": "code", "collapsed": false, "input": "!wc /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_TE_names.txt", "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": " 88 1144 7777 /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/develop_TE_names.txt\r\n" } ], "prompt_number": 70 }, { "cell_type": "code", "collapsed": false, "input": "!intersectBed -a /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_CG.gff -b /Volumes/web-1/trilobite/Crassostrea_gigas_v9_tracks/Cgigas_v9_TE.gff > /Volumes/web-1/Mollusk/bs_larvae_exp/ID_Target_Loci_Check/Genomic_Features/CG_TE_names.txt -wb", "language": "python", "metadata": 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