##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10007_H94MGADXX_V_CF71_ATCACG_R1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7948472	
Filtered Sequences	0	
Sequence length	101	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.750837267842172	34.0	31.0	34.0	30.0	34.0
2	31.84092489726327	34.0	31.0	34.0	30.0	34.0
3	31.940109872689995	34.0	31.0	34.0	30.0	34.0
4	35.26429696173051	37.0	35.0	37.0	33.0	37.0
5	35.0347486913208	37.0	35.0	37.0	33.0	37.0
6	34.755358765810584	37.0	35.0	37.0	32.0	37.0
7	34.847296058915475	37.0	35.0	37.0	32.0	37.0
8	34.86013148187475	37.0	35.0	37.0	32.0	37.0
9	36.53141207517621	39.0	37.0	39.0	33.0	39.0
10-14	36.752902218187344	39.4	37.2	39.4	32.6	39.4
15-19	37.84758310779732	41.0	38.2	41.0	33.0	41.0
20-24	37.793418483451916	41.0	39.0	41.0	33.0	41.0
25-29	37.65416163005921	40.8	38.6	41.0	32.0	41.0
30-34	37.47350907193231	40.0	38.0	41.0	32.0	41.0
35-39	37.34296091122923	40.0	38.0	41.0	31.2	41.0
40-44	37.16602549521468	40.0	38.0	41.0	31.0	41.0
45-49	36.887649273973665	40.0	37.8	41.0	30.4	41.0
50-54	36.555128985797516	40.0	37.0	41.0	30.0	41.0
55-59	36.1714357426182	39.8	36.0	41.0	29.4	41.0
60-64	35.80082632234221	39.0	35.0	41.0	29.0	41.0
65-69	35.160598741493956	37.8	35.0	40.6	28.2	41.0
70-74	34.37961459762329	36.6	34.8	39.4	27.8	41.0
75-79	33.48570152854536	35.6	34.0	38.0	26.6	39.8
80-84	32.70872238085509	35.0	34.0	36.6	26.0	38.6
85-89	32.05931431852563	35.0	34.0	35.8	25.6	37.0
90-94	31.61888116357459	35.0	33.8	35.0	24.8	36.0
95-99	31.275347312036825	35.0	33.0	35.0	23.0	36.0
100-101	30.779242224165852	35.0	32.5	35.0	18.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	162072.0
3	43234.0
4	24080.0
5	16054.0
6	14013.0
7	13723.0
8	13688.0
9	14329.0
10	15212.0
11	16156.0
12	16181.0
13	16347.0
14	16446.0
15	16554.0
16	16547.0
17	16648.0
18	16818.0
19	17855.0
20	17594.0
21	18404.0
22	20005.0
23	23198.0
24	27966.0
25	33612.0
26	40984.0
27	51173.0
28	64127.0
29	69682.0
30	82724.0
31	102604.0
32	130418.0
33	172550.0
34	245776.0
35	378772.0
36	708633.0
37	1603407.0
38	2631027.0
39	1055487.0
40	4372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.89693058602045	20.556443999879686	12.672302229755436	40.87432318434443
2	18.99292223537823	21.183990356183177	35.376163077345986	24.44692433109261
3	17.71941895985983	22.686593383140895	29.842294785406846	29.75169287159242
4	21.184656791045068	26.380677766159614	25.38139117375496	27.05327426904036
5	24.781148222563314	30.503694543766454	25.76050253146297	18.95465470220726
6	20.971528263200476	35.66457927511506	26.211862255990315	17.152030205694142
7	16.485540979052512	25.424495637785192	41.85621348884585	16.23374989431644
8	16.75202747394507	27.570674026562564	35.490083519070474	20.187214980421896
9	17.826205896135363	25.56736876490807	37.218958112181696	19.387467226774863
10-14	18.801462430937306	32.46628745936426	30.36454510552216	18.36770500417627
15-19	19.47373943665641	30.9613763004424	30.3889799154487	19.17590434745249
20-24	19.071598972131998	30.959094077765137	30.78184872948037	19.187458220622496
25-29	19.083882341903248	30.844225755974836	30.466135154158625	19.60575674796329
30-34	18.780720554991873	31.157793661563222	30.743251626957118	19.318234156487787
35-39	19.230587445931352	31.185640786616876	30.28156593524824	19.302205832203533
40-44	18.97440640333807	30.956797475307045	30.88995300804507	19.178843113309814
45-49	19.34920132137914	30.92391425112953	30.753045738349442	18.973838689141886
50-54	19.032276499819908	30.857477420622985	30.989869852527015	19.120376227030096
55-59	18.94447560520284	30.912412086544766	31.06220433645858	19.080907971793813
60-64	19.115500650356065	31.241685581593675	30.66276223180519	18.980051536245064
65-69	18.97965837779011	30.99604443083235	31.103019512666613	18.921277678710933
70-74	18.85046718223566	31.22494096788435	30.887900045150012	19.036691804729973
75-79	18.936281098280165	31.031349487255692	30.603153696462314	19.429215718001828
80-84	18.938643603273214	31.044134298193633	30.893052441623475	19.124169656909675
85-89	19.064258355810367	30.88252077164213	31.175585997705074	18.877634874842432
90-94	19.00753976729762	30.822066343349245	31.064388326350223	19.10600556300291
95-99	19.147974717618595	30.97280708855691	30.774305330034306	19.104912863790187
100-101	19.495047729881897	30.990227562237564	30.723210719172155	18.791513988708385
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.77125377036488
2	43.43984656647084
3	47.47111183145225
4	48.23793106008543
5	43.735802924770574
6	38.12355846889462
7	32.71929087336895
8	36.93924245436696
9	37.21367312291023
10-14	37.16916743511358
15-19	38.6496437841089
20-24	38.25905719275449
25-29	38.68963908986654
30-34	38.098954711479664
35-39	38.53279327813489
40-44	38.15324951664788
45-49	38.32304001052103
50-54	38.15265272685
55-59	38.02538357699665
60-64	38.095552186601125
65-69	37.900936056501045
70-74	37.88715898696564
75-79	38.365496816282
80-84	38.062813260182885
85-89	37.941893230652795
90-94	38.11354533030053
95-99	38.252887581408785
100-101	38.28656171859028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	234.0
1	172.0
2	96.0
3	88.5
4	100.5
5	114.5
6	135.0
7	175.0
8	227.5
9	280.0
10	358.5
11	472.0
12	606.5
13	771.0
14	1068.0
15	1565.0
16	3158.5
17	4069.5
18	5960.0
19	12236.0
20	17738.5
21	22321.5
22	31220.5
23	46428.0
24	68430.0
25	85796.5
26	105993.5
27	129799.0
28	155028.0
29	194166.5
30	245137.5
31	291869.5
32	331128.5
33	356765.0
34	372159.0
35	377845.0
36	364217.5
37	371806.5
38	383024.5
39	381446.5
40	376648.0
41	350700.0
42	326543.0
43	295634.5
44	264453.0
45	243652.5
46	224845.0
47	211223.5
48	187666.5
49	164328.0
50	141344.5
51	119145.0
52	110396.5
53	99319.0
54	83616.0
55	71393.0
56	59338.0
57	49448.0
58	40843.5
59	34399.5
60	28883.5
61	23118.5
62	17954.0
63	14109.0
64	10779.5
65	7917.5
66	5960.5
67	4578.5
68	3480.0
69	2702.5
70	2089.5
71	1648.5
72	1244.0
73	867.0
74	776.5
75	831.5
76	710.0
77	480.0
78	358.0
79	315.0
80	272.5
81	186.0
82	100.0
83	37.0
84	20.0
85	8.5
86	5.0
87	3.5
88	2.5
89	1.0
90	0.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033000053343586036
2	0.2264837820401204
3	0.020381275797411127
4	0.02645791543330592
5	0.051003513631299194
6	0.7568498700127521
7	0.002717503439654817
8	0.08420486352597077
9	0.018066365459927392
10-14	0.026938510949022654
15-19	0.027620403015824926
20-24	0.01346422306073419
25-29	0.019384857869537694
30-34	0.0241505537164879
35-39	0.02050708614183959
40-44	0.017198274083370994
45-49	0.012045082375581117
50-54	0.01116944237835901
55-59	0.010037149278502836
60-64	0.014256828230633511
65-69	0.00804934583653311
70-74	0.014943752711212921
75-79	0.010643555138648031
80-84	0.014148631334425031
85-89	0.01291065754524895
90-94	0.006723304806257102
95-99	0.010500131345999583
100-101	0.0012203603409560982
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7948472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	69.07146084042701
#Duplication Level	Relative count
1	100.0
2	31.22857865801762
3	15.708600740689624
4	9.960205184827364
5	7.513859176960413
6	5.788650426701631
7	4.7500747590458445
8	3.8564166264117956
9	3.4113127688450304
10++	47.81013502633819
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC	10191	0.1282133220070474	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	9013	0.11339286343337437	TruSeq Adapter, Index 1 (100% over 50bp)
CCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT	7985	0.1004595600261283	No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	7979	0.10038407381947122	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	558540	2.8160534	6.014786	1
CCCCC	501895	2.3003256	14.388274	1
CCCCA	593835	1.7335559	15.432546	1
CTGGG	554220	1.7104023	6.806586	1
CCCCT	571430	1.6654555	10.517435	1
CCCAG	541245	1.6104528	8.246465	2
CCTGC	529065	1.5716687	6.0462127	1
GCCCA	517365	1.5393987	6.385927	1
CTGCA	788765	1.4924341	5.281964	1
GCCCC	315680	1.4747064	11.900356	1
CCCAA	792160	1.4729247	6.4404263	2
CCTCC	504635	1.4707788	6.8122363	1
CCTGG	482825	1.4619205	5.6766796	1
GGCCC	305645	1.4553177	8.948555	9
TGGCC	475730	1.4404379	6.796236	8
CCCTG	483590	1.4365784	7.4779453	1
GCCTG	457845	1.3862848	5.072934	1
CTCCC	475150	1.3848436	9.655629	1
CTCCT	732710	1.3579869	8.516072	1
CCCTC	463145	1.3498545	5.2757273	1
CCAGT	708380	1.3403366	6.568191	3
CTCCA	719375	1.3354293	6.412736	1
CGGGG	257615	1.2743118	6.189853	1
CCCAC	435600	1.2716275	5.196239	1
TCCTG	669230	1.2642149	6.197345	2
CCCGG	256555	1.2215774	6.8126984	1
CTGGA	632785	1.2203538	5.2231474	2
CCGGG	250400	1.2152256	5.4805074	1
CCCCG	255605	1.1940646	10.225946	1
CCTGT	619705	1.1706592	5.8720202	3
CGCCC	243555	1.1377728	5.118653	1
GCCCT	381125	1.1321902	5.0796857	1
CCTCG	373305	1.1089599	6.5663176	1
GTCCA	553570	1.0474182	5.0813594	1
CTCGG	325705	0.9861851	7.751502	1
GTCCT	500935	0.9462957	5.77597	1
CTCCG	299655	0.89017123	6.819146	1
CCCGC	186025	0.86902004	6.041055	1
>>END_MODULE