##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10007_H94MGADXX_V_CF71_ATCACG_R2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7948472	
Filtered Sequences	0	
Sequence length	101	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194780959157935	34.0	31.0	34.0	28.0	34.0
2	31.29745440381497	34.0	31.0	34.0	28.0	34.0
3	31.38687913853128	34.0	31.0	34.0	28.0	34.0
4	34.68452640960426	37.0	35.0	37.0	32.0	37.0
5	34.669300464290494	37.0	35.0	37.0	32.0	37.0
6	34.67442761325699	37.0	35.0	37.0	32.0	37.0
7	34.667413812365446	37.0	35.0	37.0	32.0	37.0
8	34.64458590279993	37.0	35.0	37.0	32.0	37.0
9	36.33346094695936	39.0	37.0	39.0	32.0	39.0
10-14	36.592851984633015	39.4	37.6	39.4	32.0	39.4
15-19	37.64610478592615	41.0	38.2	41.0	32.0	41.0
20-24	37.60440519888603	41.0	39.0	41.0	32.0	41.0
25-29	37.47550145487083	41.0	38.6	41.0	32.0	41.0
30-34	37.33006457090118	41.0	38.0	41.0	31.4	41.0
35-39	37.048670084011114	40.0	38.0	41.0	30.2	41.0
40-44	36.93526222398469	40.0	38.0	41.0	30.0	41.0
45-49	36.57955536611314	40.0	37.6	41.0	30.0	41.0
50-54	36.271835190461765	40.0	36.8	41.0	28.8	41.0
55-59	35.93413921568825	39.8	35.8	41.0	28.0	41.0
60-64	35.375679652642674	39.0	35.0	41.0	27.6	41.0
65-69	34.71850310348958	37.6	35.0	40.2	26.4	41.0
70-74	33.94143614017889	36.4	34.0	39.2	26.0	41.0
75-79	33.017776473264306	35.4	34.0	38.0	25.0	39.8
80-84	32.17182288621008	35.0	33.2	36.4	23.8	38.4
85-89	31.74129706942416	35.0	33.4	35.8	23.8	37.0
90-94	31.314358080395827	35.0	33.2	35.0	21.2	36.0
95-99	31.211924556065618	35.0	34.0	35.0	20.2	36.0
100-101	30.852347281339107	35.0	33.5	35.0	9.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	227872.0
3	31940.0
4	19422.0
5	15201.0
6	13940.0
7	13745.0
8	14379.0
9	15635.0
10	16330.0
11	16935.0
12	17144.0
13	17463.0
14	17694.0
15	18019.0
16	17812.0
17	17769.0
18	17882.0
19	18427.0
20	18195.0
21	18908.0
22	20689.0
23	24236.0
24	28774.0
25	33831.0
26	40831.0
27	49335.0
28	59338.0
29	71504.0
30	88426.0
31	110146.0
32	137933.0
33	177612.0
34	248908.0
35	378659.0
36	698404.0
37	1567594.0
38	2622719.0
39	1021486.0
40	3335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.438249591949315	20.116021464791505	12.51615476766042	39.92957417559876
2	20.700157248427075	20.56926802028437	35.177704827851635	23.55286990343692
3	19.560860924558597	22.194652650471575	29.365590194036585	28.878896230933243
4	23.079635639318795	25.712407043393014	24.80052300528043	26.40743431200776
5	24.886225430083744	30.35493685656217	25.68152642572774	19.077311287626344
6	21.049544339426653	35.53688855707842	26.15867245464843	17.2548946488465
7	16.64358286768825	25.323935505478495	41.83285867901701	16.199622947816252
8	16.80518631891789	27.55250195333689	35.455071109526855	20.187240618218368
9	17.714958490170567	25.609301869778207	37.2601434217542	19.415596218297022
10-14	18.80515199442631	32.49845308261675	30.425437675213807	18.270957247743134
15-19	19.486302149765528	30.946793348546336	30.453376704434497	19.11352779725364
20-24	19.113944432450587	30.92537243189803	30.81299858289983	19.14768455275155
25-29	19.046739989760727	30.95641018748933	30.46959759023658	19.527252232513362
30-34	18.785862341378	31.25175502562605	30.747091366719143	19.215291266276804
35-39	19.174616065871554	31.300634458048915	30.30762827396039	19.217121202119138
40-44	19.059497015481913	31.04544473518066	30.81293276721928	19.082125482118144
45-49	19.35947730023398	31.003578425898155	30.686177365273338	18.950766908594527
50-54	19.022656748706925	30.965911414779647	31.02762010472249	18.983811731790937
55-59	18.959512404745684	31.046397466629262	31.020001468963976	18.974088659661078
60-64	19.136551110039115	31.465170625225085	30.511452770109315	18.886825494626486
65-69	18.973081075904602	31.1113328217309	31.059460596869116	18.856125505495385
70-74	18.876853154916574	31.26268144295886	30.858744158898222	19.001721243226342
75-79	19.04222990298978	31.120252285808654	30.483759388223643	19.353758422977926
80-84	19.084247071700517	31.077742838061408	30.79532640966187	19.042683680576207
85-89	19.118225466069475	30.950968991945395	31.119480130441968	18.811325411543166
90-94	19.1092157458909	30.870550792564167	30.97604646374721	19.044186997797723
95-99	19.276154618930427	30.985807418119183	30.722249503155467	19.015788459794923
100-101	19.66412681234175	31.02300933689951	30.712801727362937	18.60006212339581
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	67.36782376754807
2	44.253027151863996
3	48.43975715549184
4	49.48706995132655
5	43.96353671771009
6	38.30443898827315
7	32.8432058155045
8	36.99242693713626
9	37.13055470846759
10-14	37.076109242169444
15-19	38.59982994701917
20-24	38.26162898520214
25-29	38.573992222274086
30-34	38.00115360765481
35-39	38.39173726799069
40-44	38.14162249760006
45-49	38.3102442088285
50-54	38.006468480497865
55-59	37.933601064406766
60-64	38.0233766046656
65-69	37.82920658139999
70-74	37.878574398142916
75-79	38.395988325967714
80-84	38.12693075227672
85-89	37.92955087761264
90-94	38.15340274368862
95-99	38.291943078725346
100-101	38.26418893573755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2852.0
1	1803.0
2	676.0
3	605.5
4	628.5
5	630.0
6	629.0
7	677.0
8	701.5
9	714.0
10	820.5
11	974.5
12	1134.5
13	1354.0
14	1678.5
15	2171.5
16	3794.0
17	4795.0
18	6739.0
19	12888.5
20	18605.0
21	23792.5
22	33202.5
23	48882.0
24	71373.5
25	88218.5
26	107245.5
27	130803.5
28	156229.0
29	194121.0
30	243862.5
31	291087.5
32	330550.5
33	355297.5
34	369882.0
35	376340.5
36	362259.5
37	367894.0
38	379573.5
39	379035.5
40	373255.0
41	346863.0
42	323472.5
43	292756.0
44	261779.5
45	241021.5
46	223908.0
47	211091.5
48	187518.5
49	164251.5
50	140223.5
51	118281.5
52	110086.5
53	99660.0
54	84121.5
55	71607.5
56	59739.5
57	50286.5
58	42002.5
59	35396.5
60	29808.5
61	23948.0
62	18761.5
63	15184.5
64	11666.5
65	8610.5
66	6369.5
67	4878.5
68	3873.5
69	2990.5
70	2354.0
71	1918.0
72	1474.0
73	1043.0
74	872.0
75	888.5
76	792.5
77	579.5
78	468.5
79	420.0
80	324.5
81	216.0
82	147.5
83	95.5
84	76.5
85	48.0
86	25.5
87	19.5
88	16.0
89	13.5
90	11.0
91	9.0
92	10.0
93	10.0
94	10.5
95	8.0
96	7.0
97	10.5
98	8.5
99	12.0
100	40.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33543554031517003
2	0.10278705139805486
3	0.09754076003538793
4	0.10326513070688303
5	0.11433644101658785
6	0.1368187495659543
7	0.15311118916944036
8	0.15163920813962734
9	0.11963305651702617
10-14	0.11898135893288672
15-19	0.116993555490917
20-24	0.12767485373289358
25-29	0.11620095032101767
30-34	0.15069814676330243
35-39	0.28737095632971976
40-44	0.1566389112272145
45-49	0.23280952615798356
50-54	0.33635647203638636
55-59	0.13719618059923971
60-64	0.10262601415718645
65-69	0.10306131794890892
70-74	0.09979779761443457
75-79	0.10725080241837676
80-84	0.11667399721606869
85-89	0.11094962654457359
90-94	0.12753142994024513
95-99	0.11660354342318877
100-101	0.1186894789338127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7948472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	69.14532891650198
#Duplication Level	Relative count
1	100.0
2	31.47715098664885
3	15.691921344561047
4	10.110702807312606
5	7.38748286467993
6	5.720605006393346
7	4.786370076835351
8	3.969634484903639
9	3.3441233051872503
10++	47.90286721422894
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC	11609	0.14605322884700356	No Hit
CCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT	8572	0.10784462724407912	No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	7990	0.10052246519834253	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	626790	3.0825377	8.206619	1
CCCCC	585620	2.7630394	14.415421	1
CCCCA	605790	1.808612	15.337283	1
CCCCT	580185	1.7328681	10.063681	1
CTGGG	565845	1.7326288	7.904476	1
CCCAG	533480	1.6059961	8.16166	2
GCCCC	328125	1.5610378	12.932515	1
GCCCA	512145	1.5417688	6.2249	1
CCTGC	511635	1.5408566	6.0753517	1
CCTCC	509190	1.5208237	6.577645	1
GGCCC	314140	1.5069556	8.290693	9
CCCAA	794295	1.5005739	6.2748036	2
CTGCA	778240	1.4830906	5.267369	1
CTGGC	487850	1.4814647	5.4757714	1
TGGCC	483040	1.4668584	6.411478	8
CCTGG	481475	1.4621059	5.7441053	1
CCCTG	477640	1.4384762	6.600274	1
CTCCC	481610	1.4384489	9.444488	1
CGGGG	281895	1.3748986	7.3834233	1
CTCCT	721430	1.3640207	8.082664	1
CTCCA	694665	1.3128846	6.1432767	1
CCAGT	686670	1.308586	6.6544814	3
TCCTG	659140	1.2566301	6.0346246	2
CCGGG	258880	1.2522147	5.814727	1
CCCGG	259670	1.2456586	6.977783	1
CCCCG	261335	1.2432882	9.964437	1
CTGGA	634435	1.2191141	5.5151777	2
CCTGT	618025	1.1782457	5.867778	3
CGCCC	244625	1.1637908	5.4118385	1
CCTCG	364410	1.097469	6.344393	1
GTCCA	550995	1.0500304	5.2497897	1
CTCGG	331950	1.0080398	7.759554	1
GGATG	519845	1.0072427	5.021434	4
GTCCT	497525	0.9485161	5.7184205	1
CTCCG	299725	0.90266156	6.75234	1
CCCGC	189215	0.9001809	6.409669	1
>>END_MODULE