##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10008_H94MGADXX_V_CF34_CGATGT_R2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9752597	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291749059250577	34.0	31.0	34.0	29.0	34.0
2	31.53716933038451	34.0	31.0	34.0	30.0	34.0
3	31.59274765480415	34.0	31.0	34.0	29.0	34.0
4	34.98214537112525	37.0	35.0	37.0	33.0	37.0
5	34.94068451715989	37.0	35.0	37.0	33.0	37.0
6	34.926403603060805	37.0	35.0	37.0	32.0	37.0
7	34.920295178812374	37.0	35.0	37.0	32.0	37.0
8	34.90873251504189	37.0	35.0	37.0	32.0	37.0
9	36.616863692819464	39.0	38.0	39.0	33.0	39.0
10-14	36.889089398444334	39.4	38.2	39.4	33.0	39.4
15-19	37.96199178536753	41.0	39.0	41.0	33.0	41.0
20-24	37.91977431242161	41.0	39.0	41.0	33.0	41.0
25-29	37.78324255580334	41.0	39.0	41.0	33.0	41.0
30-34	37.6319771646465	41.0	38.4	41.0	32.4	41.0
35-39	37.3478678140807	40.0	38.0	41.0	31.2	41.0
40-44	37.20430718094883	40.0	38.0	41.0	31.2	41.0
45-49	36.81809731295162	40.0	37.2	41.0	30.0	41.0
50-54	36.49618654395337	40.0	36.6	41.0	29.8	41.0
55-59	36.13146537276173	39.6	35.6	41.0	29.0	41.0
60-64	35.57309012153378	39.0	35.0	41.0	28.2	41.0
65-69	34.9170872127701	37.4	35.0	40.2	28.0	41.0
70-74	34.169403349692395	36.4	34.0	39.2	27.2	41.0
75-79	33.25122221291416	35.2	34.0	38.0	26.0	39.8
80-84	32.41491219210637	35.0	33.2	36.4	25.0	38.4
85-89	31.99786955207931	35.0	33.8	35.8	25.0	37.0
90-94	31.5679398830896	35.0	33.2	35.0	24.4	36.0
95-99	31.477777232054187	35.0	34.0	35.0	24.4	36.0
100-101	31.087852548403262	35.0	33.5	35.0	21.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	218718.0
3	33163.0
4	20758.0
5	16443.0
6	15448.0
7	15405.0
8	16299.0
9	17819.0
10	18549.0
11	19322.0
12	19855.0
13	20039.0
14	20667.0
15	20358.0
16	20564.0
17	21018.0
18	20975.0
19	21460.0
20	21964.0
21	22888.0
22	24883.0
23	29159.0
24	34825.0
25	41069.0
26	49772.0
27	60974.0
28	73640.0
29	89414.0
30	110812.0
31	141075.0
32	177545.0
33	228558.0
34	322007.0
35	501052.0
36	958204.0
37	1955536.0
38	3123250.0
39	1225061.0
40	4049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.722878588114014	20.638595686212575	15.769908017323464	36.868617708349944
2	21.26097015706699	22.382179230041245	31.05365856662773	25.30319204626404
3	20.19316699964538	22.42993205797268	29.209354743188992	28.167546199192948
4	23.26828864723093	25.570869714097082	23.72615662293448	27.434685015737507
5	24.79709394948185	29.37253646344632	25.56266694972176	20.26770263735007
6	21.53022625476021	34.9072641642429	25.91983987401188	17.642669706985007
7	17.262908387043506	25.09933083197601	41.20210357188444	16.435657209096036
8	18.21105969980351	27.49905473616716	34.16234837013102	20.12753719389831
9	18.325857724022924	25.613837658756605	35.72965519393673	20.33064942328374
10-14	19.197303392803803	31.7914062461022	30.08486685579989	18.92642350529411
15-19	19.946319320076203	30.639939125569406	29.943423588028818	19.470317966325577
20-24	19.28566493245827	30.48225640446497	30.457429590026976	19.774649073049787
25-29	19.49911901020355	30.223507788886018	30.187093817714157	20.090279383196275
30-34	19.217414383723767	30.715135680214168	30.257450027867012	19.809999908195053
35-39	19.39108609265912	30.901752614706968	30.05204920050597	19.655112092127943
40-44	19.456205598877766	30.671150481418962	30.300011210358452	19.572632709344816
45-49	19.597906293381005	30.612776814940222	30.438220413094825	19.351096478583948
50-54	19.276854805268925	30.894860008122116	30.43733723594304	19.390947950665918
55-59	19.11091882883942	30.64308617755462	30.91395132415545	19.33204366945051
60-64	19.40600895189986	30.89060925849697	30.455091176468898	19.248290613134277
65-69	19.180671540051254	30.84066807890671	30.646386193523146	19.332274187518898
70-74	19.064675245516902	30.865512848794204	30.86763339979916	19.202178505889734
75-79	19.28046652325	30.829124297448153	30.20198157744266	19.68842760185919
80-84	19.122149729727912	30.88848762194971	30.777644405385384	19.211718242936993
85-89	19.313680249088243	30.64709355473963	30.675341179010545	19.363885017161575
90-94	19.136587442536378	30.646336035322786	30.668401277517155	19.54867524462368
95-99	19.447213386939683	30.680505176383182	30.506265573184237	19.366015863492894
100-101	19.6984727045013	30.5046824914088	30.914195815143607	18.88264898894629
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.59149629646396
2	46.56416220333103
3	48.360713198838326
4	50.70297366296844
5	45.064796586831925
6	39.17289596174522
7	33.69856559613954
8	38.33859689370182
9	38.65650714730667
10-14	38.12372689809791
15-19	39.41663728640178
20-24	39.060314005508054
25-29	39.58939839339982
30-34	39.027414291918824
35-39	39.04619818478706
40-44	39.02883830822258
45-49	38.94900277196495
50-54	38.667802755934844
55-59	38.44296249828994
60-64	38.65429956503414
65-69	38.512945727570155
70-74	38.266853751406636
75-79	38.96889412510919
80-84	38.333867972664905
85-89	38.67756526624982
90-94	38.68526268716006
95-99	38.81322925043258
100-101	38.58112169344759
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3739.0
1	2289.5
2	761.5
3	716.0
4	713.5
5	699.5
6	720.5
7	721.5
8	716.0
9	747.5
10	823.0
11	896.0
12	1062.0
13	1287.5
14	1635.0
15	2254.0
16	3579.0
17	4605.5
18	6453.0
19	11277.5
20	16568.5
21	22958.0
22	32572.0
23	48045.0
24	71064.0
25	95299.5
26	125361.0
27	160246.0
28	199213.0
29	246583.5
30	300489.5
31	350734.0
32	390974.5
33	424782.5
34	452661.5
35	458595.0
36	442747.0
37	436006.5
38	428803.0
39	425801.0
40	424321.0
41	393632.0
42	356721.0
43	327608.5
44	328540.0
45	307915.5
46	282498.0
47	272249.0
48	231367.0
49	203778.5
50	184680.5
51	163737.0
52	166774.5
53	158050.0
54	124510.5
55	105442.0
56	90896.5
57	77486.0
58	66018.5
59	57955.0
60	50619.5
61	42356.5
62	34805.0
63	28895.0
64	22988.5
65	17406.0
66	13349.5
67	10251.0
68	7862.5
69	5997.0
70	4611.0
71	3582.5
72	2701.0
73	2041.0
74	1587.5
75	1333.0
76	1084.0
77	861.5
78	678.5
79	519.5
80	427.0
81	306.5
82	201.5
83	142.0
84	99.5
85	66.0
86	53.0
87	42.5
88	29.0
89	21.0
90	13.5
91	13.5
92	11.0
93	10.0
94	14.5
95	11.5
96	7.0
97	6.5
98	8.0
99	10.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3366487921114755
2	0.10517198649754522
3	0.09978880497163987
4	0.10529503064670875
5	0.11551794870638045
6	0.13602530690030565
7	0.15056502385979856
8	0.14944737283822965
9	0.11878887233831154
10-14	0.11819210821486831
15-19	0.1165248599937022
20-24	0.12634583383277295
25-29	0.1156717538928349
30-34	0.14928946618013644
35-39	0.2842463397185386
40-44	0.1555913773531296
45-49	0.23251242720272353
50-54	0.33292465586345876
55-59	0.13546955749325026
60-64	0.10294078592604616
65-69	0.10412611122965502
70-74	0.10095977512451298
75-79	0.10807582841780503
80-84	0.11741077786767976
85-89	0.11169127566739404
90-94	0.12705538842628278
95-99	0.11702729026945334
100-101	0.11814289055520287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9752597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	85.74475548441463
#Duplication Level	Relative count
1	100.0
2	86.36723611268243
3	79.62722027378663
4	59.941735490440095
5	41.64781083833013
6	27.703925783459667
7	19.247086774522003
8	14.684919108496436
9	11.599162801221858
10++	124.85575291322547
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA	28726	0.2945471857393472	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	28110	0.2882309194156182	No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	25055	0.2569059297743975	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG	20727	0.21252800664274346	No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	17970	0.18425861337241764	No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT	17860	0.18313070867175174	No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC	15140	0.1552407015280135	No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT	10435	0.10699714137680455	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	786285	2.949213	8.917768	1
CCCCC	720015	2.496202	12.932096	1
GGCCC	487970	1.7458481	20.485144	9
CTGCA	1155640	1.7367042	6.656066	2
GCAGG	720020	1.7094116	8.037061	4
CCCCA	743640	1.6840386	11.262022	1
TGCAG	1100590	1.6802211	5.58915	3
CTGGG	692490	1.644148	5.2276263	1
TGGCC	682215	1.5944504	14.393693	8
GGCCG	438150	1.5924792	5.898386	25-29
CGGCC	435700	1.5588379	5.8218493	25-29
GCCCA	667085	1.5346457	5.66173	1
CCTGG	643800	1.5046681	5.1210027	1
CCCAG	653200	1.5027027	6.488259	1
CCTCC	662120	1.4995165	5.7649336	1
CCCAA	1008690	1.4921006	5.272489	2
CTGGA	960030	1.4656345	11.507526	2
GCGGC	401100	1.4578191	5.3062134	20-24
CCCTG	632960	1.4562248	6.3076825	1
CGGGG	388690	1.4351324	6.157653	1
GCCCC	405830	1.4292887	7.7938137	1
CCCCT	626735	1.4193794	9.380249	1
ACTGG	918435	1.4021333	10.277606	1
GCCGC	388270	1.3891438	5.632214	25-29
ATGGC	905155	1.3818593	9.520418	7
TGCAT	1392435	1.3669562	7.371355	7
CCAGT	902845	1.3568022	5.646839	3
ATGCA	1367165	1.3420706	7.991444	6
CTCCA	903490	1.3365618	5.2596226	1
CTCCC	582610	1.3194486	8.570981	1
GCCCT	571050	1.313791	5.02537	1
CGCGG	341170	1.2400004	5.1124916	20-24
CCTGT	817695	1.2289094	7.442602	3
CTCCT	817420	1.209306	8.639414	1
CCCGG	334660	1.197339	5.596787	1
TCCTG	788390	1.1848673	6.6535397	2
GCATC	786010	1.1812215	9.9077015	8
GATGC	768690	1.1735244	10.5770235	5
CATCT	1179440	1.1397718	6.8998585	9
TCCTT	1167910	1.1286954	5.0365806	3
CCCCG	320235	1.1278323	7.533905	1
GGATG	704250	1.0922079	11.083866	4
CCTCG	430140	0.98960525	5.0410075	1
TGGAT	965685	0.9630587	7.851456	3
CTCGG	405060	0.9466929	5.2514954	1
GATCC	622585	0.93562526	8.744625	1
CCCGC	242980	0.8557488	5.0186133	1
ATCCT	841680	0.8133717	5.080686	2
TATGG	795110	0.7929476	5.7915664	6
GTATG	776675	0.7745627	6.00329	5
CTAGT	538795	0.52893615	5.680487	2
ACTAG	517790	0.50828594	5.5147424	1
>>END_MODULE