FastQCFastQC Report
Mon 16 Jun 2014
3291_5903_10009_H94MGADXX_V_CF26_TTAGGC_R1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename 3291_5903_10009_H94MGADXX_V_CF26_TTAGGC_R1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 19319381
Filtered Sequences 0
Sequence length 101
%GC 36

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC 128262 0.6639032585981921 No Hit
CTTAGAAACTGCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGC 112448 0.5820476339278158 No Hit
GTCCTTTCGTACTAAGAAAAGTATAACTTTCGTGGATAGAAACTGACCTG 103188 0.5341164916205131 No Hit
CCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT 97001 0.502091656042189 No Hit
CTCTCCAAAAAAATTACGCTGTTATCCCTACGGTAACTTATTCCTTTGCT 50347 0.2606035876615302 No Hit
CTTTGCTTAAAAGAAAAGCTTTAACGCTTTTATGCGAGTTAGATGCTATT 49274 0.2550495794870446 No Hit
GCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGCAAACTTTTTT 46900 0.2427614011028614 No Hit
CCCTACGGTAACTTATTCCTTTGCTCGCTATTTAGCGGATCACTTAATTT 41323 0.21389401658365764 No Hit
CCTTTGCTTAAAAGAAAAGCTTTAACGCTTTTATGCGAGTTAGATGCTAT 41070 0.21258445081651423 No Hit
CAAAAACATCGCCCCTTGCAAAATAATTAACGAATAAGGGGTCCTGCCTG 38083 0.19712329292537892 No Hit
CTGCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGCAAACTTTT 37711 0.19519776539424322 No Hit
CTCCGCGATTGCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAA 37387 0.19352069302841535 No Hit
CCCCAGTCTTGCCATTCATACCAGCCTCTAATTAAGAGGCAAATGATTAT 36717 0.19005267301266018 No Hit
CCTCGATGTTGGATCGAGATTTCCTAGAGATGCAGCAGTTTCTAAGGGTT 36568 0.18928142677035045 No Hit
CGAGAAGACCCTATCGAGCTTTAGTAAAATTGTTAGTAGCATGTAAATAT 35094 0.1816517827356891 No Hit
CTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGCAAACTTTTTTA 31808 0.16464295621065705 No Hit
CCCTTAGAAACTGCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTC 30840 0.15963244371028243 No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCCGTTTAACTACGTCACTGGG 30693 0.1588715497665272 No Hit
CCCCAGTCTTGCCATTCATACCAGCCTCTATTTAAGAGGCAAATGATTAT 29922 0.15488073867377014 No Hit
CTCTGGTCCTTTCGTACTAAGAAAAGTATAACTTTCGTGGATAGAAACTG 29852 0.15451840822436289 No Hit
GCCGAGTTCCTTTTTCTTATTTTATTAAAAAATTAATTACCTGAGTTGGA 29712 0.15379374732554837 No Hit
GCTAAATAGCGAGCAAAGGAATAAGTTACCGTAGGGATAACAGCGTAATT 29680 0.15362811054867648 No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA 29613 0.15328130854710098 No Hit
GTCGCAAACTTTTTTATCAATATGAACTCTCCAAAAAAATTACGCTGTTA 29332 0.1518268106001947 No Hit
ATTTTTGTGAAGAAGCAGAAATAAATTCGTAGGACGAGAAGACCCTATCG 28280 0.14638150156053137 No Hit
GGGCGATGTTTTTGGTAAACAGGCGAAGTCAATATTTGCCGAGTTCCTTT 27695 0.14335345423334217 No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT 26467 0.13699714292088344 No Hit
AGCTCATCAAGTTTCGGGACACAAAACACTTTTAGTAAAAGCTTGCTCAA 26349 0.13638635730616835 No Hit
GAATAAGTTACCGTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATAT 25107 0.12995757990382817 No Hit
ACCAGCTATAACTTGATGCGATTAGCATTTCACATCTAATGTATCCTCAA 24199 0.12525763636008833 No Hit
GCTACTAACAATTTTACTAAAGCTCGATAGGGTCTTCTCGTCCTACGAAT 24176 0.12513858492671168 No Hit
GCCCCGACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC 23352 0.12087343792226055 No Hit
CACCATTAACTAAAATTAATTTCTTAGAAAACCAGCTATAACTTGATGCG 23020 0.11915495636221471 No Hit
CCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCTT 22836 0.11820254489520135 No Hit
ATAAATTCGTAGGACGAGAAGACCCTATCGAGCTTTAGTAAAATTGTTAG 22425 0.11607514754225304 No Hit
AGAAGACCCTATCGAGCTTTAGTAAAATTGTTAGTAGCATGTAAATATAA 21508 0.11132861865501799 No Hit
AGATAACTTTGGTTGGGGCAATCGCGGAGAATATAAATCCTCCGCTAAAA 21403 0.1107851229809071 No Hit
GCCGTTTAACTACGTCACTGGGCAGGCAGGACCCCTTATTCGTTAATTAT 20662 0.10694959636646742 No Hit
CCCCGACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT 20086 0.10396813438277346 No Hit
TATAAATCCTCCGCTAAAAGTATTAAAAATTACTATTTTTAAATTAAGTG 19952 0.10327453037962242 No Hit
GCTCGCTATTTAGCGGATCACTTAATTTAAAAATAGTAATTTTTAATACT 19899 0.10300019446792834 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 1182780 3.381463 8.513684 1
TTTTT 19203780 3.0831795 4.592081 2
GGGGC 1017210 2.880632 8.770261 2
CGCGG 981420 2.753015 7.512227 20-24
CCCCC 1009455 2.7521381 12.169813 1
GCCCC 960555 2.6438022 48.451576 1
GCAGG 1521125 2.4199624 8.569608 2
CCCCT 1550345 2.3989289 8.418898 1
CCAAC 2702455 2.3698552 15.788256 4
GACCC 1487905 2.322587 6.712867 7
AGGGG 1442580 2.316899 5.068883 35-39
TGGGG 1436810 2.3093104 7.966535 8
CTGGG 1449365 2.3074763 10.060545 1
GTTGG 2528270 2.284487 6.3469048 8
GCCGG 808200 2.2671096 9.093044 55-59
CCGCG 808570 2.2467144 12.036744 3
CCCCA 1446540 2.2366793 33.486427 1
GGCAG 1370075 2.1796565 8.152922 1
TCCGC 1373165 2.145039 9.118861 2
GGCCG 758810 2.1285641 7.322227 75-79
TTCCT 4235675 2.1111698 5.402685 65-69
CCTGC 1345380 2.101636 6.9295897 1
GCTGC 1330510 2.0982351 9.675553 1
GGGGT 1280165 2.0575428 5.061883 3
GGGCA 1288350 2.04964 5.159313 1
CTTCC 2287775 2.0091302 5.6507645 1
CGGCC 719910 2.0003612 7.437034 75-79
CCCTA 2273450 1.9950992 8.403832 1
CCCTT 2218560 1.9483455 6.1372514 1
CCTTT 3900925 1.9443218 9.121477 3
CAGGC 1208740 1.9048164 5.280625 20-24
TCGAG 2125440 1.9009683 5.2897677 30-34
GCGAG 1187395 1.8890305 5.311122 9
TGGGC 1182230 1.8821813 6.5668507 95-97
CCTCC 1212645 1.8763882 6.508146 1
GGGCG 659820 1.8685412 9.829818 1
CCCAG 1177860 1.8386134 15.456562 2
CCAAA 3576910 1.7789403 10.374543 8
GGCGA 1117185 1.777333 6.374829 2
GGTCG 1114535 1.7744064 7.30024 45-49
CGCCG 629995 1.750521 9.170904 55-59
CGAGG 1090215 1.7344264 6.7862406 40-44
GCCTG 1098950 1.7330612 5.8605547 1
CAACC 1965055 1.7232093 14.659995 5
CGCTG 1076830 1.6981776 5.6826134 1
TCTCC 1915815 1.682474 5.575803 2
CTTTC 3370270 1.6798296 7.226525 4
AAGCT 3342670 1.6795236 5.125264 45-49
GCCCA 1056100 1.6485487 5.2323236 1
CGCCC 596890 1.6428618 6.6824217 1
CCTCG 1033080 1.6137878 10.72829 1
CTGGC 1023035 1.6133422 5.848916 45-49
CTCTC 1827435 1.6048584 7.0030184 1
CTGCT 1809940 1.6046578 11.400238 9
CAAAG 3180150 1.5967041 10.306101 9
CCTAC 1814040 1.5919371 5.3221407 2
TTGCC 1793620 1.5901887 12.078604 9
TCCTA 3164270 1.5760077 5.067891 65-69
CCTGG 997820 1.5735776 5.3298306 45-49
AAAGT 5483570 1.5625935 5.00861 9
CCAGC 995875 1.5545388 6.538433 2
ACCAA 3121970 1.5526805 10.593234 7
CTCCG 969725 1.5148201 9.654777 1
TCCTT 3033895 1.5121716 8.590841 2
CCTAG 1698920 1.5051349 7.60628 65-69
CCGGG 536145 1.5039588 8.693247 1
CTCCT 1709885 1.5016257 6.789887 1
CTTTG 2966840 1.4928567 5.752972 1
CTCCA 1685125 1.4788058 5.767444 1
CCCAA 1669470 1.464003 14.282303 3
TCGCC 933300 1.4579202 7.764568 9
CTCGC 930140 1.452984 5.2642393 1
CCCGG 518895 1.4418155 5.897495 1
GCGGC 506325 1.4203099 6.566969 75-79
TCCTG 1600320 1.4188128 5.7626777 2
CGGGG 498035 1.410383 8.839536 1
CTGCA 1591135 1.4096442 7.563629 2
CCCCG 510965 1.4063644 14.287157 1
CTTGC 1580865 1.4015644 8.722654 8
CTCCC 899250 1.391456 7.687409 1
ATCCC 1561400 1.3702294 6.141695 95-97
GCTCG 868165 1.3691096 5.4022036 20-24
TCCCT 1552230 1.3631727 5.755233 95-97
GTCCT 1510580 1.339251 14.12413 1
CGGTC 843690 1.3305123 5.9517026 60-64
CCGAG 826890 1.3030708 5.991338 2
TGCTG 1455470 1.3027017 5.5954986 2
GAAAC 2561020 1.2858486 6.769793 5
CTCGG 811485 1.2797244 8.866381 1
GCATC 1434790 1.2711326 5.557263 4
CCGGT 789340 1.2448014 5.533525 55-59
GGCCC 445515 1.2379199 13.750685 9
GTCGC 782870 1.2345982 6.9338717 45-49
CCCTG 790245 1.2344521 6.134142 1
AACCA 2473720 1.2302798 9.496511 6
ACTGC 1383820 1.2259766 11.21265 8
CACCA 1365020 1.1970226 5.057827 1
GATGC 1336835 1.1956494 5.1036534 5
TGGCC 753125 1.1876899 8.874211 8
GTCTT 2348465 1.1817024 5.6939893 6
CGATG 1315145 1.17625 6.9924254 4
ATGGC 1310090 1.171729 5.7895513 7
ACGCC 729340 1.1384836 5.263567 55-59
CGTGG 710365 1.1309439 5.198633 30-34
GGTCC 706970 1.1149025 5.928175 5
GCCGA 704235 1.1097825 5.9130125 1
GCTAC 1252555 1.1096841 6.7667203 95-97
GATGT 2163910 1.0984265 5.129274 5
TTCGT 2168470 1.0911323 7.3038497 6
AACTG 2148020 1.0792719 6.555134 7
TCGGC 676050 1.0661414 5.023691 2
CTACT 2130720 1.0612341 6.0036607 95-97
CATGC 1197490 1.0609001 7.189637 90-94
CCAGT 1182255 1.0474026 9.577818 3
GCCGT 639600 1.0086591 5.7570114 1
CGGGC 351835 0.9869446 5.2538757 1
GCGAT 1062485 0.950274 5.9829464 5
GCCCT 597265 0.93299556 5.4815254 1
GCTCA 1047360 0.9278943 5.3494925 2
GTACT 1794985 0.9025455 6.650821 9
TCTTG 1769965 0.8906123 5.753365 7
CCCGA 568340 0.8871662 5.5014234 2
CTGGA 942065 0.84257174 5.1268663 2
CTCTG 887810 0.78711516 5.0121775 1
CCCGC 277275 0.7631632 5.7815266 1
TCGTA 1508245 0.7583683 7.3091364 7
CTTAG 1454700 0.7314451 6.9563603 1
TTTCG 1445780 0.72748876 6.120075 5
CAGTC 800850 0.7095021 7.899076 4
CGCGA 436535 0.6879222 6.5915694 4
CGTAC 543855 0.48182097 9.987052 8