##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10009_H94MGADXX_V_CF26_TTAGGC_R2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	19319381	
Filtered Sequences	0	
Sequence length	101	
%GC	36	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258891058673154	34.0	31.0	34.0	28.0	34.0
2	31.416743269362513	34.0	31.0	34.0	28.0	34.0
3	31.544849599477335	34.0	31.0	34.0	28.0	34.0
4	34.891863667888735	37.0	35.0	37.0	32.0	37.0
5	34.85328965767589	37.0	35.0	37.0	32.0	37.0
6	34.81792967383375	37.0	35.0	37.0	32.0	37.0
7	34.81933691353776	37.0	35.0	37.0	32.0	37.0
8	34.80229728892453	37.0	35.0	37.0	32.0	37.0
9	36.50326369152304	39.0	37.0	39.0	33.0	39.0
10-14	36.769572803600695	39.4	37.6	39.4	32.8	39.4
15-19	37.84241077910312	41.0	38.2	41.0	33.0	41.0
20-24	37.818610472043595	41.0	39.0	41.0	33.0	41.0
25-29	37.69027608079161	41.0	39.0	41.0	32.0	41.0
30-34	37.5508885921345	41.0	38.0	41.0	32.0	41.0
35-39	37.26462055901273	40.0	38.0	41.0	31.0	41.0
40-44	37.1719926844447	40.0	38.0	41.0	30.8	41.0
45-49	36.80474754341249	40.0	37.8	41.0	30.0	41.0
50-54	36.51510554090734	40.0	37.0	41.0	29.6	41.0
55-59	36.20671775146419	39.8	36.0	41.0	29.0	41.0
60-64	35.61417608566238	39.0	35.2	41.0	28.2	41.0
65-69	34.95052989534189	37.4	35.0	40.2	28.0	41.0
70-74	34.22539714911156	36.4	34.4	39.4	27.4	41.0
75-79	33.327487231604366	35.4	34.0	38.0	26.0	40.0
80-84	32.49708319329693	35.0	33.8	36.6	25.2	38.8
85-89	32.088728681317484	35.0	34.0	36.0	25.2	37.0
90-94	31.644001699640377	35.0	33.6	35.2	24.6	36.2
95-99	31.51168439609944	35.0	34.0	35.0	24.4	36.0
100-101	31.156556542883024	35.0	33.5	35.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	460017.0
3	67805.0
4	40755.0
5	32770.0
6	31260.0
7	31389.0
8	33319.0
9	35934.0
10	38058.0
11	40463.0
12	40946.0
13	41726.0
14	42073.0
15	42080.0
16	42547.0
17	41693.0
18	42814.0
19	43809.0
20	43653.0
21	45754.0
22	49583.0
23	59427.0
24	71818.0
25	83782.0
26	100114.0
27	119937.0
28	145117.0
29	175324.0
30	212925.0
31	267373.0
32	334180.0
33	432172.0
34	604086.0
35	912408.0
36	1661171.0
37	3867836.0
38	6377788.0
39	2594291.0
40	11184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.00432957317659	20.962140645686034	14.540224362886375	36.493305418251
2	20.278843627760104	20.949207275335088	35.12580426693501	23.646144829969796
3	18.59097700299254	22.87955995136708	31.03772937226696	27.491733673373425
4	21.978199182093654	26.732117897818398	26.474181313800806	24.81550160628715
5	24.08637865145632	31.5649670802891	26.143576849954254	18.20507741830032
6	19.725306783574514	37.42619653058381	26.36506353795524	16.483433147886437
7	15.712324223292448	26.594576861051234	42.009584554222734	15.683514361433584
8	15.546568279793322	28.935552699314087	34.64735302729798	20.87052599359461
9	16.866338816783212	27.222513687721406	37.27908575043976	18.632061745055626
10-14	18.363100944853123	33.43360761245073	30.916712564930492	17.286578877765656
15-19	18.1622765220364	32.05935632799093	31.64409603407109	18.134271115901573
20-24	18.113180250992254	32.28280288936447	32.17301725641735	17.430999603225924
25-29	17.620562652765493	32.9903967047337	30.598262883939697	18.790777758561113
30-34	17.368826852236076	32.74379529411474	32.34546386267589	17.541913990973285
35-39	18.443852306374573	33.18776180863251	30.972476204651954	17.395909680340964
40-44	17.778682861280927	32.19988889102838	32.53792648452506	17.48350176316564
45-49	18.926936098822516	32.12879800993528	31.978681880454474	16.965584010787733
50-54	17.483561155642253	31.627722423579673	33.53519366762581	17.353522753152262
55-59	17.53248552695554	32.221981461403104	32.84794127573038	17.397591735910975
60-64	18.171467364570123	33.067697939097776	31.03159277092419	17.729241925407916
65-69	17.159721208737743	32.45369996988928	32.886889577218874	17.49968924415411
70-74	17.215658864942956	33.859883064823435	31.769750053023945	17.154708017209668
75-79	17.76743134114295	32.7682413993295	31.2596738524991	18.204653407028452
80-84	18.031680570263948	32.59350710316392	31.97731842566702	17.397493900905115
85-89	17.899441292376043	32.063041953085495	33.001615332203905	17.035901422334558
90-94	17.84187697619105	31.435808450926615	32.89413651693959	17.828178055942743
95-99	18.222858385483566	31.974747941700087	31.872417537403468	17.929976135412875
100-101	18.259384993380763	32.54484570106086	32.36486119501192	16.830908110546456
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.49763499142759
2	43.9249884577299
3	46.08271067636596
4	46.7937007883808
5	42.29145606975664
6	36.20873993146095
7	31.395838584726032
8	36.41709427338793
9	35.498400561838835
10-14	35.64967982261878
15-19	36.29654763793798
20-24	35.544179854218186
25-29	36.411340411326606
30-34	34.91074084320936
35-39	35.83976198671554
40-44	35.26218462444656
45-49	35.892520109610246
50-54	34.83708390879451
55-59	34.93007726286652
60-64	35.90070928997804
65-69	34.65941045289185
70-74	34.370366882152624
75-79	35.972084748171405
80-84	35.42917447116906
85-89	34.9353427147106
90-94	35.67005503213379
95-99	36.152834520896434
100-101	35.09029310392722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7922.0
1	5157.5
2	2209.5
3	2085.5
4	2164.5
5	2231.0
6	2363.0
7	2451.0
8	2529.5
9	2797.5
10	3128.5
11	3602.0
12	4286.0
13	5065.0
14	6161.0
15	8260.5
16	21456.0
17	25929.5
18	41589.0
19	100081.5
20	139667.0
21	150663.5
22	191013.0
23	285826.5
24	422693.0
25	451525.5
26	448240.5
27	419083.5
28	393553.5
29	516486.5
30	721707.5
31	889904.5
32	956452.0
33	902813.5
34	889704.0
35	871066.5
36	743053.0
37	823291.5
38	977045.0
39	999264.5
40	920512.0
41	780009.5
42	760949.5
43	675530.5
44	538153.5
45	498948.5
46	466092.0
47	456796.5
48	395799.0
49	316723.0
50	214774.0
51	130053.5
52	119097.5
53	109286.5
54	86450.5
55	72329.5
56	59508.5
57	49101.0
58	40847.5
59	36013.5
60	31426.0
61	25489.0
62	20530.5
63	16664.5
64	13064.5
65	9912.0
66	7657.0
67	6154.0
68	4715.0
69	3452.0
70	2765.5
71	2233.5
72	1642.5
73	1176.5
74	912.5
75	826.5
76	728.0
77	572.5
78	443.5
79	344.0
80	255.0
81	176.5
82	134.0
83	88.5
84	61.0
85	47.0
86	32.5
87	25.5
88	25.0
89	16.0
90	10.5
91	11.5
92	12.0
93	11.5
94	10.5
95	10.0
96	7.5
97	13.5
98	19.5
99	22.0
100	77.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3403369911282354
2	0.10741027365214237
3	0.10194943616464731
4	0.10786577478854008
5	0.1192533031984824
6	0.14288760079838997
7	0.15966867675522314
8	0.1585402762127834
9	0.1250971757324937
10-14	0.12510959849075912
15-19	0.12330001670343373
20-24	0.13345044543611412
25-29	0.12230930173176874
30-34	0.15719137171113298
35-39	0.29222571882608456
40-44	0.16195964042533248
45-49	0.23621874841642182
50-54	0.3407862808855004
55-59	0.1436857630169414
60-64	0.10934304779226622
65-69	0.11011015311515415
70-74	0.10637918471611486
75-79	0.11388460116812231
80-84	0.1236840869798054
85-89	0.11733916319575663
90-94	0.1340084343282013
95-99	0.12342527951594308
100-101	0.12479178292513617
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.9319381E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	83.90962546953628
#Duplication Level	Relative count
1	100.0
2	37.24531963060117
3	17.38713811731168
4	9.821888437720842
5	6.7172134583827425
6	5.025932181058129
7	4.11081964856804
8	3.3526177728269073
9	2.79923820475044
10++	53.09808712733719
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC	144086	0.745810644761341	No Hit
CTTAGAAACTGCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGC	110876	0.5739107272639843	No Hit
GTCCTTTCGTACTAAGAAAAGTATAACTTTCGTGGATAGAAACTGACCTG	107226	0.5550177824020345	No Hit
CCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT	106475	0.5511304942948224	No Hit
GCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGCAAACTTTTTT	52790	0.2732489203458434	No Hit
CTCTCCAAAAAAATTACGCTGTTATCCCTACGGTAACTTATTCCTTTGCT	50188	0.259780579926448	No Hit
CTTTGCTTAAAAGAAAAGCTTTAACGCTTTTATGCGAGTTAGATGCTATT	47713	0.24696961046526286	No Hit
CCCTACGGTAACTTATTCCTTTGCTCGCTATTTAGCGGATCACTTAATTT	41325	0.2139043688822121	No Hit
CTGCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGCAAACTTTT	40795	0.21116100976527147	No Hit
CCCCAGTCTTGCCATTCATACCAGCCTCTAATTAAGAGGCAAATGATTAT	40277	0.20847976443965777	No Hit
CTCCGCGATTGCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAA	39872	0.20638342398237294	No Hit
CCTTTGCTTAAAAGAAAAGCTTTAACGCTTTTATGCGAGTTAGATGCTAT	39658	0.2052757280370422	No Hit
GCCGAGTTCCTTTTTCTTATTTTATTAAAAAATTAATTACCTGAGTTGGA	35079	0.1815741404965304	No Hit
CGAGAAGACCCTATCGAGCTTTAGTAAAATTGTTAGTAGCATGTAAATAT	33784	0.17487102718249617	No Hit
CTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTCGCAAACTTTTTTA	33701	0.1744414067924847	No Hit
CCTCGATGTTGGATCGAGATTTCCTAGAGATGCAGCAGTTTCTAAGGGTT	33497	0.17338547233992643	No Hit
CCCCAGTCTTGCCATTCATACCAGCCTCTATTTAAGAGGCAAATGATTAT	33351	0.17262975454544843	No Hit
GCTACCTTTGCACGGTCAAAATACCGCGGCCGTTTAACTACGTCACTGGG	32833	0.16994850921983473	No Hit
CAAAAACATCGCCCCTTGCAAAATAATTAACGAATAAGGGGTCCTGCCTG	32242	0.16688940499698204	No Hit
GGGCGATGTTTTTGGTAAACAGGCGAAGTCAATATTTGCCGAGTTCCTTT	31822	0.1647154223005385	No Hit
CTCTGGTCCTTTCGTACTAAGAAAAGTATAACTTTCGTGGATAGAAACTG	31604	0.16358702175809878	No Hit
GTCGCAAACTTTTTTATCAATATGAACTCTCCAAAAAAATTACGCTGTTA	30272	0.15669239092080642	No Hit
CCCTTAGAAACTGCTGCATCTCTAGGAAATCTCGATCCAACATCGAGGTC	30037	0.1554759958406535	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	29748	0.15398008869952923	No Hit
GCTAAATAGCGAGCAAAGGAATAAGTTACCGTAGGGATAACAGCGTAATT	27557	0.14263914563308214	No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	26141	0.13530971825650107	No Hit
GCCCCGACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC	25587	0.1324421315569065	No Hit
CCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCTT	24892	0.1288447078092202	No Hit
GAATAAGTTACCGTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATAT	24293	0.1257441943921495	No Hit
AGCTCATCAAGTTTCGGGACACAAAACACTTTTAGTAAAAGCTTGCTCAA	23874	0.12357538784498322	No Hit
ATTTTTGTGAAGAAGCAGAAATAAATTCGTAGGACGAGAAGACCCTATCG	23472	0.12149457583553014	No Hit
GCCGTTTAACTACGTCACTGGGCAGGCAGGACCCCTTATTCGTTAATTAT	23254	0.1203661752930904	No Hit
GCTACTAACAATTTTACTAAAGCTCGATAGGGTCTTCTCGTCCTACGAAT	22783	0.11792820898350728	No Hit
ACCAGCTATAACTTGATGCGATTAGCATTTCACATCTAATGTATCCTCAA	22421	0.11605444294514404	No Hit
CCCCGACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT	22248	0.11515896912018041	No Hit
CACCATTAACTAAAATTAATTTCTTAGAAAACCAGCTATAACTTGATGCG	21647	0.11204810340455523	No Hit
AGATAACTTTGGTTGGGGCAATCGCGGAGAATATAAATCCTCCGCTAAAA	21627	0.11194458041901031	No Hit
AGAAGACCCTATCGAGCTTTAGTAAAATTGTTAGTAGCATGTAAATATAA	20964	0.10851279344819588	No Hit
CTGGGGTTAAGCTGTCTCTTTCTTAATACTTGAATTTTATATTTTTGTGA	20608	0.10667008430549613	No Hit
CTTTCGTACTAAGAAAAGTATAACTTTCGTGGATAGAAACTGACCTGGCT	20137	0.10423211799591302	No Hit
GGGCAGGCAGGACCCCTTATTCGTTAATTATTTTGCAAGGGGCGATGTTT	20055	0.10380767375517881	No Hit
GCTCGCTATTTAGCGGATCACTTAATTTAAAAATAGTAATTTTTAATACT	19810	0.1025395171822534	No Hit
CTGGTCCTTTCGTACTAAGAAAAGTATAACTTTCGTGGATAGAAACTGAC	19694	0.10193908386609281	No Hit
TGCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAG	19585	0.10137488359487294	No Hit
AAAGTATTAAAAATTACTATTTTTAAATTAAGTGATCCGCTAAATAGCGA	19503	0.10095043935413872	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	1339350	3.6911366	10.8413	1
CCCCC	1151080	3.2541585	12.599269	1
AAAAA	19823395	3.148251	4.906727	65-69
GGGGC	1099465	3.0455158	10.662783	2
TTTTT	18446580	3.0002127	4.3543878	2
CGCGG	990695	2.758245	7.741347	20-24
GCCCC	973485	2.738098	54.117176	1
GCAGG	1599015	2.502978	8.669151	2
CCCCT	1565725	2.5005138	8.051741	1
CCAAC	2673225	2.388867	17.259506	4
CTGGG	1517240	2.3863149	11.913057	1
TGGGG	1505915	2.3564622	8.316163	8
CCCCA	1478805	2.3504753	35.9445	1
GCCGG	837035	2.3304322	9.355244	55-59
GACCC	1462300	2.3124259	6.7409425	7
CCGCG	818730	2.2911158	12.782531	3
GGCAG	1458050	2.282322	8.43903	1
GTTGG	2522500	2.241224	5.8112087	8
TCCGC	1375755	2.185956	9.438307	2
GGCCG	782900	2.1797123	7.607628	75-79
TTCCT	4254760	2.168453	5.8272495	65-69
GGGCA	1338755	2.0955865	6.2078733	1
GCTGC	1311825	2.0737808	10.861877	1
CGGCC	739645	2.0698059	7.8504066	75-79
GGGGT	1314385	2.0567555	5.883766	3
CCTGC	1287755	2.0461316	6.9694414	1
GGGCG	738360	2.0452557	11.457464	1
CAGGC	1294840	2.037202	5.8875804	20-24
CCCTA	2223180	1.9961816	8.296202	1
CCTTT	3904560	1.9899724	9.327375	3
CCCTT	2196240	1.9814091	5.849352	1
GGGGA	1252585	1.9507351	5.7347717	1
CTTCC	2160270	1.9489576	5.255876	1
GGCGA	1239865	1.9407917	7.3989415	2
TGGGC	1219065	1.9173452	6.8860865	95-97
CCTCC	1197070	1.9117597	6.166152	1
CCGGC	668835	1.8716531	5.383936	1
TCGAG	2081650	1.850147	5.2568865	30-34
CCCAG	1166250	1.8442639	16.149927	2
CGCCG	648950	1.8160069	9.783821	55-59
CCAAA	3589310	1.8033459	11.386059	8
GGTCG	1138590	1.7907742	6.9733996	45-49
AGCTT	3491950	1.7622218	5.0510764	45-49
CGAGG	1124320	1.7599261	6.975988	30-34
CAACC	1945725	1.738753	16.324749	5
CTTTC	3396215	1.7308927	7.466169	4
TCTCC	1882345	1.6982186	5.4683867	2
AAGCT	3365450	1.6903114	5.3934536	45-49
GCCTG	1065535	1.6844364	5.615513	1
CGCTG	1050540	1.6607318	5.904078	1
CTGGC	1048490	1.6574912	6.0565724	45-49
TTGCC	1836315	1.6482692	12.79931	9
GCCCA	1031745	1.6315624	5.1352544	1
CTCTC	1804295	1.6278032	6.9644737	1
CCTGG	1021435	1.6147215	5.5870285	45-49
CGCCC	572800	1.6111008	7.2316456	1
CAAAG	3186020	1.5925868	11.152719	9
CCTAC	1758185	1.578665	5.2918	2
ACCAA	3126435	1.5707875	11.470088	7
TCCTA	3094935	1.5698478	5.4278626	65-69
CCTCG	987280	1.5687028	9.938493	1
CTGCT	1741450	1.5631187	11.318887	9
TCCTT	3056220	1.557613	8.831912	2
CCAGC	982990	1.5544634	5.979183	2
AAAGT	5489085	1.5500124	5.242407	9
CCGGG	552590	1.5384946	9.19794	1
CTCCG	966455	1.5356137	10.162965	1
CCCAA	1692165	1.5121648	15.827765	3
CGGGG	543950	1.5067405	10.088486	1
CCTAG	1683000	1.5034746	8.215201	65-69
CTCCT	1659610	1.497271	6.61014	1
CTTTG	2951545	1.4966178	5.6670256	1
GCGGC	536370	1.4933355	6.9723105	75-79
CTCCA	1632240	1.4655796	5.7182417	1
CCCCG	512975	1.4428326	14.434618	1
CCCGG	513710	1.4375545	6.2669935	1
CTCGC	900960	1.4315478	5.668119	1
TCCTG	1590050	1.4272226	5.6703243	2
CTGCA	1592215	1.4223735	7.825463	2
CAGCC	894030	1.4137852	5.1673617	20-24
CTCCC	882365	1.4091656	7.643144	1
TCGCC	875320	1.390808	6.7825274	9
TCCCT	1536140	1.3858786	5.8145847	95-97
ATCCC	1542565	1.3850608	6.120023	95-97
CTTGC	1541685	1.3838103	9.134736	8
CGGTC	873225	1.3804259	6.404762	60-64
GTCCT	1512995	1.3580583	14.66735	1
GCTCG	854600	1.3509827	5.161943	1
CCGAG	850100	1.3374821	6.9417768	2
TGCTG	1448370	1.2934422	5.8350024	2
GAAAC	2581180	1.2902473	6.601098	5
CTCGG	815230	1.2887452	8.849594	1
CCGGT	805040	1.2726367	5.7033396	55-59
ACTGG	1428815	1.2699146	5.3314567	1
GCATC	1418220	1.2669386	6.1732907	4
GTCGC	793710	1.2547257	6.8119917	45-49
GGCCC	448200	1.2542328	12.007685	9
AACCA	2472030	1.2420006	10.355564	6
CCCTG	765355	1.216083	5.5724673	1
ACTGC	1353760	1.2093545	11.054088	8
TGGCC	763315	1.2066761	7.8582296	8
GTGGG	769665	1.2043751	5.2455764	1
GCCGA	752935	1.18461	6.9484124	1
CGATG	1329290	1.181458	7.0236325	4
ATGGC	1317875	1.1713125	5.223432	7
ACGCC	731705	1.1570905	5.6169586	55-59
GATGC	1298420	1.154021	5.479631	5
GTCTT	2272100	1.1520969	5.9635644	6
GCTAC	1282520	1.1457137	7.43333	95-97
CGTGG	720455	1.1331315	5.468548	30-34
GGTCC	707775	1.1188765	6.170765	5
TTCGT	2196580	1.1138034	7.2659736	6
GATGT	2185305	1.0972135	5.1191754	5
CATGC	1217965	1.0880448	7.8110113	90-94
AACTG	2141360	1.075507	6.401281	7
CTACT	2106370	1.0684166	6.608842	95-97
CGGGC	372375	1.0367485	6.682529	1
TCGGC	655140	1.0356692	5.076784	2
GCCGT	647105	1.0229671	6.181899	1
CCAGT	1141455	1.0196961	10.000706	3
GCGAT	1101300	0.97882307	6.522468	5
GCCCT	591925	0.9405177	5.5878944	1
GTACT	1820405	0.9186723	6.838634	9
GCTCA	1017680	0.9091242	5.0605035	2
CCCGA	570505	0.9021751	5.6357636	2
TCTTG	1749045	0.88687515	5.938412	7
CTGGA	948430	0.84295386	5.651307	2
CGGGA	523440	0.8193537	5.878824	1
CTCTG	880435	0.790275	5.0003815	1
CCCGC	280655	0.78939164	6.5079355	1
GGATG	872470	0.77149945	5.396371	4
TCGTA	1502780	0.7583819	7.253103	7
TTTCG	1464340	0.7425119	6.324889	5
CTTAG	1407560	0.7103289	6.779087	1
CAGTC	780910	0.6976104	8.37154	4
CGCGA	435655	0.6854261	6.878741	4
CGTAC	554175	0.4950612	10.405604	8
>>END_MODULE