##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10010_H94MGADXX_HK_CF2_TGACCA_R1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	23172864	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90987484326495	34.0	31.0	34.0	30.0	34.0
2	31.996141521393298	34.0	31.0	34.0	30.0	34.0
3	32.093480892133144	34.0	31.0	34.0	30.0	34.0
4	35.424519601892975	37.0	37.0	37.0	35.0	37.0
5	35.22211837949767	37.0	35.0	37.0	33.0	37.0
6	34.943121704766405	37.0	35.0	37.0	33.0	37.0
7	35.03756415262266	37.0	35.0	37.0	33.0	37.0
8	35.054412307429935	37.0	35.0	37.0	33.0	37.0
9	36.73895345866614	39.0	38.0	39.0	33.0	39.0
10-14	36.971221442459594	39.4	38.2	39.4	33.0	39.4
15-19	38.078821987649	41.0	39.0	41.0	33.0	41.0
20-24	38.01757624780433	41.0	39.0	41.0	33.0	41.0
25-29	37.85994147292281	41.0	38.8	41.0	33.0	41.0
30-34	37.67468401834145	40.0	38.0	41.0	32.8	41.0
35-39	37.50884096156608	40.0	38.0	41.0	32.0	41.0
40-44	37.25398274464477	40.0	38.0	41.0	31.4	41.0
45-49	36.9180809847242	40.0	37.2	41.0	30.8	41.0
50-54	36.56086706416609	40.0	36.4	41.0	30.0	41.0
55-59	36.146401221704835	39.6	35.2	41.0	29.6	41.0
60-64	35.7802276921834	39.0	35.0	41.0	29.4	41.0
65-69	35.176824780916164	37.6	35.0	40.4	29.0	41.0
70-74	34.445109469420785	36.4	34.8	39.4	28.6	41.0
75-79	33.566443871590494	35.4	34.0	38.0	27.2	39.8
80-84	32.81346814101183	35.0	34.0	36.6	26.4	38.6
85-89	32.18337023856869	35.0	34.0	35.8	26.0	37.0
90-94	31.74262827417448	35.0	34.0	35.0	25.2	36.0
95-99	31.392868417127897	35.0	33.4	35.0	24.4	36.0
100-101	30.847110223406137	35.0	32.5	35.0	20.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	402766.0
3	107870.0
4	63148.0
5	44052.0
6	39807.0
7	38602.0
8	38892.0
9	41625.0
10	43968.0
11	45655.0
12	46320.0
13	46259.0
14	46425.0
15	47455.0
16	47048.0
17	47615.0
18	47557.0
19	49868.0
20	51058.0
21	54045.0
22	58321.0
23	67353.0
24	80991.0
25	99672.0
26	120992.0
27	149529.0
28	191534.0
29	204571.0
30	243731.0
31	303130.0
32	387668.0
33	521274.0
34	755316.0
35	1202751.0
36	2444821.0
37	4537685.0
38	7296565.0
39	3139148.0
40	17777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.129536236623768	22.061365104465512	16.865686628148215	34.9434120307625
2	19.15014171657075	22.844045168521298	31.499176861026072	26.506636253881876
3	18.097245652354445	22.869680870808452	31.59343343963434	27.43964003720276
4	21.806988847891738	26.707451154679195	24.732284351328985	26.75327564610008
5	25.33428702876353	29.221609067883747	25.47317500619122	19.970928897161503
6	21.133044328282395	34.95903674722615	26.836141684304458	17.07177724018699
7	17.445588522339655	25.921166067291296	40.45300616743898	16.180239242930067
8	18.635294432667248	27.457207460597736	33.91738821638782	19.99010989034719
9	19.081613886493052	25.642244131432296	35.00034507847525	20.275796903599403
10-14	19.420549385867663	31.264719639440596	29.899179286378168	19.415551688313574
15-19	20.513765335549042	30.442004891587356	29.497184974372182	19.54704479849142
20-24	19.254734758814244	30.220246011427456	30.538186359513052	19.986832870245248
25-29	19.802224111827833	29.620327362130293	30.09478338529365	20.482665140748217
30-34	19.017380124348186	30.652019885992253	30.425273592273967	19.905326397385597
35-39	19.4994380389273	30.658046247001618	29.865668876286072	19.976846837785015
40-44	19.514179748491507	30.60543055093836	30.438195478215075	19.442194222355056
45-49	19.562748239379875	30.459351818752005	30.57520384008428	19.40269610178384
50-54	19.07974954290944	30.75856981465418	30.58255699711135	19.579123645325037
55-59	19.043957106687728	30.3232119278661	31.311390831356494	19.32144013408968
60-64	19.471715489891665	30.514566551881916	30.929722965609187	19.08399499261723
65-69	19.008931069856647	30.78464061655558	30.72028171354796	19.486146600039813
70-74	19.026794327231432	30.890087571208447	31.030718496038332	19.05239960552178
75-79	19.18479182397218	30.753099651614285	30.333170102157535	19.728938422255997
80-84	19.027330532596608	30.983087420776638	31.027737272146656	18.961844774480095
85-89	19.56005405840919	30.61911373191354	30.455699137810736	19.36513307186653
90-94	19.06300893072572	30.562126833764676	30.837728943464192	19.537135292045416
95-99	19.68519803010275	30.540251420832696	30.60216223552688	19.17238831353767
100-101	19.68761214720722	30.27656979334447	31.12054468652466	18.915273372923654
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	61.07294826738627
2	45.65677797045263
3	45.53688568955721
4	48.560264493991816
5	45.30521592592503
6	38.20482156846939
7	33.62582776526972
8	38.625404323014436
9	39.35741079009246
10-14	38.83610107418124
15-19	40.06081013404046
20-24	39.241567629059496
25-29	40.28488925257606
30-34	38.92270652173378
35-39	39.47628487671231
40-44	38.95637397084656
45-49	38.96544434116371
50-54	38.65887318823448
55-59	38.365397240777405
60-64	38.5557104825089
65-69	38.49507766989646
70-74	38.07919393275322
75-79	38.91373024622818
80-84	37.98917530707671
85-89	38.925187130275724
90-94	38.600144222771135
95-99	38.85758634364042
100-101	38.60288552013087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3016.0
1	2418.0
2	1603.5
3	1409.0
4	1486.0
5	1609.0
6	1760.0
7	1888.5
8	2185.0
9	2594.0
10	3043.0
11	3761.5
12	4435.5
13	5203.5
14	6105.0
15	7394.0
16	10481.5
17	13338.5
18	19125.0
19	31769.5
20	45547.5
21	59495.0
22	80223.0
23	115479.0
24	171287.5
25	229175.5
26	300731.0
27	383152.0
28	469224.0
29	575331.5
30	704600.5
31	834979.0
32	945628.5
33	1026047.5
34	1063715.0
35	1051117.0
36	1015387.0
37	1017672.5
38	1022851.0
39	1001078.5
40	993951.0
41	929102.0
42	829805.0
43	789144.0
44	885407.0
45	816351.0
46	656063.5
47	624653.5
48	512294.0
49	431491.5
50	393366.0
51	346631.0
52	377561.5
53	371232.0
54	280526.5
55	241596.0
56	215446.0
57	189145.5
58	169611.0
59	155818.0
60	140040.0
61	119223.5
62	98611.5
63	81320.5
64	66892.0
65	52898.5
66	40878.0
67	31619.0
68	24112.5
69	18179.5
70	13740.0
71	10334.0
72	7561.0
73	5382.0
74	4186.5
75	3634.0
76	2828.0
77	1940.5
78	1341.5
79	1046.0
80	815.5
81	516.0
82	301.5
83	168.5
84	108.0
85	50.0
86	28.0
87	20.0
88	12.5
89	14.0
90	10.0
91	3.0
92	2.5
93	2.5
94	1.5
95	1.5
96	1.5
97	0.5
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03243448889183486
2	0.22012384830809004
3	0.020247820899479668
4	0.026811532661651143
5	0.047607408389398906
6	0.7506970221721407
7	0.0029560437587688774
8	0.07948521166826854
9	0.01821527110330428
10-14	0.026738170991725495
15-19	0.02760297561837846
20-24	0.013144685093737226
25-29	0.019326916172295316
30-34	0.023999623007324427
35-39	0.020330676432572165
40-44	0.01743332200974381
45-49	0.01203649233862504
50-54	0.011304601796307958
55-59	0.009587075641578012
60-64	0.013961157326086234
65-69	0.008053385200897049
70-74	0.014768998773738109
75-79	0.010588246666445719
80-84	0.014007763563450768
85-89	0.012785644450336394
90-94	0.006423029971608171
95-99	0.010517474231929209
100-101	8.4581690031927E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.3172864E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	84.391665313976
#Duplication Level	Relative count
1	100.0
2	75.99939123376623
3	62.65219155844156
4	43.28835227272727
5	27.582183441558442
6	18.45829951298701
7	13.220373376623376
8	10.595068993506494
9	8.570921266233766
10++	146.93841314935065
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	155891	0.672730828610568	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	139621	0.6025193950993715	No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA	77254	0.3333813204962494	No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT	74393	0.32103498298699723	No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT	66005	0.2848374719672113	No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC	62344	0.26903882057910494	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG	61587	0.2657720685712392	No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGA	47907	0.206737501242833	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	32998	0.1423993167180371	TruSeq Adapter, Index 4 (100% over 50bp)
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT	25885	0.11170393094267503	No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT	24597	0.10614570559771981	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	2242150	3.5354156	11.133234	10-14
TTTTT	19341225	3.2959123	4.5382895	95-97
AAAAA	18305505	3.2368717	4.285457	65-69
CCCCC	1671895	2.4199388	12.141609	1
GCAGG	1996035	1.9973836	14.556551	4
GGGGA	1845765	1.8789093	6.4011583	15-19
TGCAG	2924735	1.8755256	9.889176	3
GGCCG	1217070	1.8544674	8.533432	25-29
CGGCC	1228200	1.8396561	8.379994	25-29
CTGCA	2891100	1.8224831	10.962679	2
GGCCC	1194775	1.7895906	38.22523	9
GCGGC	1146215	1.7465045	8.01582	20-24
CCCCA	1782990	1.6948606	11.052858	1
GCCGC	1129060	1.6911596	8.128736	25-29
TGGCC	1664190	1.6249865	25.960999	8
CGGGG	1024295	1.5876865	9.173176	10-14
CCTCC	1677275	1.5826283	5.65527	1
CAGGA	2424755	1.5664437	9.276418	5
GCCCA	1618815	1.565375	5.226167	1
TCTGC	2489770	1.5579345	9.073196	1
CGCGG	1020220	1.5545242	7.668636	20-24
CCTGG	1581665	1.5444055	5.0892334	1
CCCAG	1577700	1.5256172	6.661319	1
CTGGA	2317110	1.4858779	16.544687	2
CCGGG	950250	1.4479098	8.408848	10-14
CTCCA	2329345	1.4434379	5.3231626	1
CCCGG	955720	1.4315227	8.397833	10-14
CCCTG	1455890	1.3974597	6.4073467	1
ACTGG	2172555	1.3931801	15.451548	1
GCCCC	940700	1.3851048	7.841817	1
ATGCA	3328605	1.3780098	11.346413	6
ATGGC	2145735	1.3759813	17.166014	7
CCCCT	1441475	1.3601342	8.317033	1
CCCAC	1405140	1.335687	5.272423	1
TGCAT	3245170	1.3335739	10.596764	7
CTCCC	1396125	1.3173431	6.9730744	1
GCCCG	868720	1.3012099	8.227605	10-14
CCAGT	2062725	1.3002946	5.019667	3
CTCCT	2084020	1.2819047	6.480139	1
TCCTT	2924550	1.1727142	5.3146577	3
CCTAC	1874820	1.16178	5.8485928	4
CATCT	2867095	1.1582056	10.079071	9
GATGC	1791655	1.148923	15.727017	5
GCATC	1811860	1.1421549	14.963738	8
GGATG	1724455	1.1249274	16.172564	4
GTTCC	1760955	1.10189	5.316969	9
CCCCG	746005	1.0984322	7.329865	1
GATCC	1697640	1.0701532	11.810511	1
AGGAT	2315555	0.97517216	5.948079	6
TGGAT	2310615	0.96592486	11.128596	3
ATCCT	2329160	0.9408987	7.3463454	2
TCGCG	940280	0.9181296	5.1900835	20-24
TATGG	2058310	0.8604518	10.791726	6
CTACG	1342260	0.8461299	5.190079	5
GGATA	1980915	0.834242	5.8606973	7
AGTAT	2930000	0.7907459	7.214298	4
GTATG	1870730	0.78203624	10.970614	5
CGTTC	1231360	0.77050424	5.098425	8
TAGTA	2429005	0.6555378	6.936389	3
CTAGT	1409685	0.57929766	10.627083	2
ACTAG	1368745	0.56664705	10.599375	1
>>END_MODULE